CN103120188A - Omphalia lapidescens Schroet plant-virus-resisting agent and preparation method thereof - Google Patents
Omphalia lapidescens Schroet plant-virus-resisting agent and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a microorganism-derived virus-resisting agent which takes Omphalia lapidescens Schroet extract as an active component and a preparation method thereof. The preparation method comprises the following steps: pulverizing kernels of Omphalia lapidescens Schroet; and through a certain processing course, preparing Omphalia lapidescens Schroet virus-resisting soluble liquid, water and microemulsion which can be used for controlling tobacco mosaic virus (TMV) diseases, cucumber mosaic virus (CMV) diseases, potato virus X and Y (PVX and PVY) diseases and other multiple plant virus diseases on tobaccos, chilies, tomatoes, vegetable marrows, potatoes and other crops. The preparation contains 10-30% of Omphalia lapidescens Schroet extract and the balance of assistant. The plant-virus-resisting agent disclosed by the invention has favorable virus-resisting effect, and is safe to the environment, humans, livestock, natural enemies of pests and other beneficial organisms. The preparation process of the plant-virus-resisting agent is simple, low in cost and suitable for popularization.
Description
Technical field
The invention belongs to microorganism and application thereof, be specifically related to stone-like omphalia anti-plant viral disease preparation and preparation method thereof.
Background technology
Plant virus is a kind ofly to infect active cytozoicus pathogen by having of forming of nucleic acid and protein, and according to the 8th report of ICTV (ICTV), the plant virus kind found of the whole world reaches 1122 at present.The caused plant disease of plant virus have the title of " plant cancer ", and its extent of injury to crops is only second to the mycosis original, and almost every kind of crops all are subject to the harm of 2～3 kinds of viruses.Approximately 400 hundred million dollars of the losses that is caused by plant virus in whole world every year, only the harm that causes of tobacco mosaic virus (Tobacco mosaic virus, TMV) is just over 100,000,000 dollars.
Because plant virus has obligate parasitism to plant cell, the needed matter and energy of virus replication place is provided by host plant fully, causes virus and host's metabolism to combine together; Plant lacks complete immune system in addition, in a single day virus infects plant, just can survive indefinite duration in plant cell, until the host is dead.Therefore, the medicine of control of plant virosis only is difficult to optionally challenge virus and does not injure host cell, causes efficient, single-minded, the safe anti-virus formulation research of screening at present to face great difficulty.
Be cross protection except taking traditional Anti-virus Disease Breeding, attenuated strain at present, improve the methods such as culture technique, insecticide control biography virus mediator and transgenosis, the scientific research personnel has also worked out the chemical prevention and control method of some viral diseases of plants.But up to the present, only have the sub-fraction anti-plant virus agent to obtain practical application, these preparations are greatly mainly with putting prevention first, and how undesirable drug effect is.Because people study early antivirotic in medicine or veterinary drug, some anti-plant virus agents (as agricultural virazole) are exactly to develop from anti-human or zoosis toxic agent.Therefore, further investigated Antiphytoviral natural materials is conducive to research, exploitation and the application of anti-plant virus agent.
Stone-like omphalia (Omphalia lapidescens Schroet) is the dry sclerotia of the white mushroom of Eumycota section fungi stone-like omphalia, claims again bamboo Siberian cocklebur, bamboo grove, bamboo tinkling of pieces of jade sesame, cotton rose.Stone-like omphalia is the anthelmintic drug that Shennong's Herbal is put down in writing, on NORTHWEST CHINA, southwest, south China and other places, distribution is arranged all, mainly economize output with Henan, Gansu, Shaanxi, Anhui etc. more, natural stone-like omphalia sclerotium often is born near the bamboo root, and so far stone-like omphalia medicine source still take wild as main.
Application on the traditional Chinese medical science and veterinary drug only has fragmentary report at present about stone-like omphalia.The traditional Chinese medical science thinks, the stone-like omphalia bitter cold is slightly poisonous, and the merit of killing insect and eliminating accumulation is arranged.report, stone-like omphalia is made the capsulae enterosolubilis formulation, at its treatment infantile ascariasis of clinical middle application, the efficient 95%(medium cloud China of reaching, Wang Xu, Li Kelei. the application [J] of stone-like omphalia capsulae enterosolubilis in the treatment infantile ascariasis. medical science selected works .2001, 20 (2): 205-206.), stone-like omphalia protease has antitumor action (YAO Y H, MA H F.The extraction of protease of Omphalia lapideacens schroet and180cases of its effect on the inhibition of mouse sarcoma[J] .Journal of Ning xia Medical College, 1979, 1:50-52.), stone-like omphalia is slightly carried polysaccharide and is had hypoglycemic activity (ZHANG G Q, HUANG Y D, BIAN Y, et a.l Hypoglycemic activity of the fungi Cordyceps militaris, Cordyceps sinensis, Tricholoma mongolicum, and Omphalia lapidescens in streptozotoc in-induced diabetic rats[J] .Applied Microbiology﹠amp, Biotechnology, 2006,72 (6): 1152-1156.), simultaneously, on veterinary drug, stone-like omphalia to kill the chicken tapeworm have preferably effect (Liu Jinxin. the Chinese drugs omphalia various dose is killed chicken tapeworm effect observation [J]. Guizhou animal and veterinary .1996,20 (2): 25-26.).And the research of stone-like omphalia aspect the Antiphytoviral effect so far there are no the report, the application on agricultural also awaits further exploitation.
Summary of the invention
The object of the invention is to provides high, nuisanceless, cheap microbial source anti-plant virus agent of a kind of Antiphytoviral effect and preparation method thereof take stone-like omphalia (Omphalia lapidescens Schroet) as raw material.
In order to realize above-mentioned task, the present invention takes following technical solution:
A kind of stone-like omphalia anti-plant virus agent is characterized in that, this stone-like omphalia anti-plant virus agent that makes is take Omphalia extract as principal component and adds auxiliary agent to process, and the preparation form is soluble liquid, aqua and microemulsion; Wherein:
Described microemulsion is prepared by following raw materials by weight:
Omphalia extract: 10%～30%, organic solvent: 30%～60%, surfactant: 5%～30%, antifreezing agent: 4%～10%, surplus is water, the percentage by weight sum of raw material is 100%;
Described soluble liquid is prepared by following raw materials by weight:
Omphalia extract: 10%～30%, organic solvent: 30%～60%, surfactant: 5%～30%, the percentage by weight sum of raw material is 100%;
Described aqua is prepared by following raw materials by weight:
Omphalia extract: 10%～30%, organic solvent: 30%～60%, surfactant: 5%～30%, antifreezing agent: 4%～10%, surplus is water, the percentage by weight sum of raw material is 100%.
Above-mentioned organic solvent is ethyl acetate, cyclohexanone, methyl oleate, ethanol, methyl alcohol, acetone, the mixture of one or more in dimethyl formamide;
Described emulsifier is one or more the mixture in alkyl benzene sulfonate, alkylphenol polyoxyethylene class, multi-styrene phenol APEO, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers, this Pan's series, tween, agricultural anion-phosphate;
Described surfactant is one or more mixture in the addition product, aralkyl phenol polyethenoxy ether formaldehyde condensation products of alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers, castor oil and oxirane;
Described antifreezing agent is ethylene glycol or glycerine.
The preparation method of above-mentioned stone-like omphalia anti-plant virus agent is characterized in that:
The preparation method of described microemulsion is:
A, the stone-like omphalia sclerotium is minced with the meal that the plant tissue cracker is crushed to 1～5mm.
B, the meal of steps A gained is minced with conventional solvent method lixiviate, extraction solvent is any one or a few in ethanol, ethyl acetate, chloroform, benzinum, water, extracting temperature is 50～80 ℃, extraction time is 6～12 hours, extract through Vacuum Concentration to being equivalent to 1kg dry powder/kg concentrate.
C, with the concentrate of step B gained, (the still temperature that is mixed is 35-60 ℃ in the still that is mixed in proportion according to formula ratio and organic solvent, surfactant and antifreezing agent, 800～1000 rev/mins of mixing speeds, mixing time are 30 minutes) namely get stone-like omphalia Antiphytoviral microemulsion after stirring.
The preparation method of described soluble liquid is:
A, the stone-like omphalia sclerotium is minced with the meal that cracker is crushed to 1～5mm.
B, the meal of steps A gained is minced with conventional solvent method lixiviate, extraction solvent is any one or a few in ethanol, ethyl acetate, chloroform, benzinum, water, extracting temperature is 50～80 ℃, extraction time is 6～12 hours, extract through Vacuum Concentration to being equivalent to 1kg dry powder/kg concentrate.
C, with the concentrate of step B gained, (the still temperature that is mixed is 35-60 ℃ in the still that is mixed in proportion according to formula ratio and organic solvent and surfactant, 800～1000 rev/mins of mixing speeds, the time is 30 minutes) namely get stone-like omphalia Antiphytoviral soluble liquid after stirring.
The preparation method of described aqua is:
A, the stone-like omphalia sclerotium is minced with the meal that cracker is crushed to 1～5mm.
B, the meal of steps A gained is minced with conventional solvent method lixiviate, extraction solvent is any one or a few in ethanol, ethyl acetate, chloroform, benzinum, water, extracting temperature is 50～80 ℃, extraction time is 6～12 hours, extract through Vacuum Concentration to being equivalent to 1kg dry powder/kg concentrate.
C, with the concentrate of step B gained, (the still temperature that is mixed is 35 ℃～60 ℃ in the still that is mixed in proportion according to formula ratio and organic solvent, surfactant, antifreezing agent and water, 800～1000 rev/mins of mixing speeds, mixing time are 30 minutes) namely get stone-like omphalia Antiphytoviral aqua after stirring.
The made stone-like omphalia anti-plant virus agent of the present invention has the following advantages:
1. anti-phytoviral activity is remarkable, can be used for preventing and treating on tobacco, capsicum, tomato, custard squash, potatoes and other crops tobacco mosaic virus disease (Tobacco mosaic virus, TMV), Cucumber Mosaic Virus (Cucumber mosaic virus, CMV) and potato virus disease (Potato Virus X, PVX; Potato Virus Y, PVY) etc. the various plants virus disease;
2. to people, animal, pest natural enemy and other beneficial organism safety, environment compatibility is good;
3. biogenic pesticide, use rear noresidue to poison;
4. simple, with low cost, suitable the promoting the use of of aboundresources, preparation method.
Embodiment
In order better to understand essence of an invention, the below describes the technology contents of invention in detail with embodiment, but invention is not limited to these embodiment.
Anti-plant virus agent of the present invention is take the stone-like omphalia sclerotium as raw material, obtains Omphalia extract through traditional solvent method lixiviate, adds auxiliary agent in Omphalia extract, through certain process, makes stone-like omphalia soluble liquid, aqua or microemulsion.
Microemulsion is prepared by following raw materials by weight:
Omphalia extract: 10%～30%, organic solvent: 30%～60%, surfactant: 5%～30%, antifreezing agent: 4%～10%, surplus is water, the percentage by weight sum of raw material is 100%;
The following raw materials by weight preparation of soluble liquid:
Omphalia extract: 10%～30%, organic solvent: 30%～60%, surfactant: 5%～30%, the percentage by weight sum of raw material is 100%;
The following raw materials by weight preparation of aqua:
Omphalia extract: 10%～30%, organic solvent: 30%～60%, surfactant: 5%～30%, antifreezing agent: 4%～10%, surplus is water, the percentage by weight sum of raw material is 100%.
Above-mentioned organic solvent is ethyl acetate, cyclohexanone, methyl oleate, ethanol, methyl alcohol, acetone, the mixture of one or more in dimethyl formamide;
Described emulsifier is one or more the mixture in alkyl benzene sulfonate, alkylphenol polyoxyethylene class, multi-styrene phenol APEO, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers and similar substance thereof, this Pan's series, tween, agricultural anion-phosphate;
Described surfactant is one or more mixture in the addition product, aralkyl phenol polyethenoxy ether formaldehyde condensation products of alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers and similar substance, castor oil and oxirane;
Described antifreezing agent is ethylene glycol or glycerine.
The preparation method of above-mentioned stone-like omphalia anti-plant virus agent, adopt following steps:
1, the acquisition of Omphalia extract:
A, the stone-like omphalia sclerotium is minced with the meal that the plant tissue cracker is crushed to 1～5mm.
B, the meal of steps A gained is minced adopt the solvent method lixiviate, extraction solvent is any one or a few in ethanol, ethyl acetate, chloroform, benzinum, water, extracting temperature is 50～80 ℃, extraction time is 6～12 hours, extract through Vacuum Concentration to being equivalent to 1kg dry powder/kg concentrate; Be Omphalia extract.
2, the preparation of stone-like omphalia anti-plant virus agent
1) with the concentrate of step B gained, be placed according to formula ratio and organic solvent and surfactant the still that is mixed, the still temperature that is mixed is 35 ℃～60 ℃, mixing time is 30 minutes, namely gets stone-like omphalia Antiphytoviral microemulsion after stirring;
2) with the concentrate of step B gained, be placed according to formula ratio and organic solvent, antifreezing agent and surfactant the still that is mixed, the still temperature that is mixed is 35 ℃～60 ℃, and mixing time is 30 minutes, namely gets stone-like omphalia Antiphytoviral soluble liquid after stirring;
3) with the concentrate of step B gained, be placed according to formula ratio and organic solvent, surfactant, antifreezing agent and water the still that is mixed, the still temperature that is mixed is 35 ℃～60 ℃, and mixing time is 30 minutes, namely gets stone-like omphalia Antiphytoviral aqua after stirring.
Through inventor's evidence, 20% stone-like omphalia microemulsion, 20% stone-like omphalia soluble liquid and 10% stone-like omphalia aqua have good control efficiency to tobacco mosaic virus (TMV).
Below the embodiment that the inventor provides.
The preparation of embodiment 1:20% stone-like omphalia microemulsion
Take stone-like omphalia concentrate 20kg, be dissolved in 30kg ethyl acetate, then add the 15kg calcium dodecyl benzene sulfonate, 5kg styrene acid APEO, mixed under high-speed stirred, be heated to 40 ℃ of left and right; Drip deionized water 30kg under high-speed stirred, control the rate of addition of water, make temperature remain on 40 ℃ of left and right; Water dropwises, and is warming up to 50 ℃, stirs 1 hour, makes 20% stone-like omphalia microemulsion 100kg.The stability of preparation, outward appearance etc. meet the requirement of commodity preparation.
The preparation of embodiment 2:20% stone-like omphalia soluble liquid
Take stone-like omphalia concentrate 20kg, be dissolved in 30kg ethyl acetate, add again the 5kg alkylphenol polyoxyethylene, 1kg APG A, 5kg ethylene glycol, the ethanol polishing is to 100kg, after mixing, stirred 10～30 minutes with 800～1000 rev/mins of mixing speeds, can make stone-like omphalia soluble liquid 100kg.The stability of preparation, outward appearance etc. meet the requirement of commodity preparation.
The preparation of embodiment 3:10% stone-like omphalia aqua
Take stone-like omphalia concentrate 20kg, be dissolved in 30kg ethyl acetate, add again the 5kg alkylphenol polyoxyethylene, 1kg APG A, 5kg ethylene glycol, the water polishing is to 100kg, after mixing, stirred 10～30 minutes with 800～1000 rev/mins of mixing speeds, can make stone-like omphalia soluble liquid 100kg.The stability of preparation, outward appearance etc. meet the requirement of commodity preparation.
The indoor bioassay of embodiment 4:3 kind stone-like omphalia antivirotic to TMV
(1) to the external passivation effect of TMV
Adopt the half withered spot method of leaf to measure, choose 5～6 leaf phase Nicotiana glutinosas (Nicotiana glutinosa) that growth is vigorous, growing way is consistent and be withered spot host.Take vein as the boundary, Zuo Banye inoculation liquid and viral equal-volume mixed liquor, right half leaf inoculation distilled water and viral equal-volume mixed liquor compare, and virus inoculation concentration is 10 μ g/mL, and passivation time is 5min, rinses the inoculation blade surface with clear water immediately after inoculation.4 leaves of every processing inoculation, test repeats 3 times, adds up withered spot number after 3d, calculates inhibiting rate.
Inhibiting rate (%)=(contrast withered spot number-processings withered spot number)/contrast withered spot number * 100
Measurement result sees Table 1.
Table 1:3 kind stone-like omphalia antivirotic is to the TMV inactivation in vitro
(2) inhibition that TMV is just infected
Adopt the half withered spot method of leaf, choosing 5～6 consistent, healthy leaf phase Nicotiana glutinosas of growing way is withered spot host, virus inoculation after smearing liquid 6h, 12h, 24h, blank is that clear water is processed, and each processes 4 leaves of inoculation, repeats 3 times, add up withered spot number after 3d, calculate inhibiting rate.The results are shown in Table 2.
The inhibition that table 2:3 kind stone-like omphalia antivirotic just infects TMV
The inhibition of (3) TMV being bred
Adopt the half withered spot method of leaf, choosing 5～6 consistent, healthy leaf phase Nicotiana glutinosas of growing way is withered spot host, smears liquid after virus inoculation 6h, 12h, 24h, blank is that clear water is processed, and each processes 4 leaves of inoculation, repeats 3 times, add up withered spot number after 3d, calculate inhibiting rate.The results are shown in Table 3.
The inhibition of table 3:3 kind stone-like omphalia antivirotic to TMV propagation
By table 1,2,3 as can be known, the passivation to TMV of 3 kinds of stone-like omphalia antivirotics is just infected, proliferation inhibiting effect is good, apparently higher than contrast medicament moroxydine. second copper.
The pot experiment of 43 kinds of stone-like omphalia antivirotics of embodiment to TMV
(1) to the protective effect of common cigarette
Select the 5-6 leaf phase, consistent, the healthy and strong common cigarette (Nicotiana tabacum) of growing way supplies examination.2 groups of prevention group (dispenser 3 times then inoculate TMV) and control groups (only inoculating not dispenser) are established in test.With 200 times of liquid sprayings of 3 kinds of stone-like omphalia antivirotics, contrast medicament moroxydine. common cigarette is processed in 500 times of liquid sprayings of second copper, and every 5d dispenser once is total to dispenser three times.Inoculate TMV after last dispenser 24h, inoculum density is 1:20 (W/V).After inoculation, 14d, 21d check incidence, statistics disease index and control efficiency.Every processing 60 strain cigarette seedlings, test repeats 3 times.Calculate preventive effect.
The incidence of disease=(the total strain number of diseased plant number/processing of getting a haircut everywhere) * 100%
Disease index=∑ (sick progression * diseased plant number)/(the highest sick level * respectively process total strain number) * 100
Before disease index growth rate=(after the spray medicine, disease refers to-spray that disease refers to before medicine)/spray medicine disease refer to * 100%
Control efficiency=(the contrast disease refers to-process that disease refers to)/contrast disease to refer to * 100%
The protective effect measurement result is as shown in table 4.
The protective effect measurement result of table 4:3 kind stone-like omphalia antivirotic to common cigarette
(2) to the therapeutic action of common cigarette
Select the 5-6 leaf phase, consistent, the healthy and strong common cigarette (Nicotiana tabacum) of growing way supplies examination.2 groups for the treatment of group (inoculation TMV after dispenser 3 times) and control groups (only inoculating not dispenser) are established in test.Inoculate malicious source TMV, inoculum density is 1:20 (W/V), and after inoculation, every 5d dispenser once, is total to dispenser three times.Medicament is 200 times of liquid sprayings of 3 kinds of stone-like omphalia antivirotics, contrast medicament moroxydine. 500 times of liquid sprayings of second copper.Check incidence during respectively at 14d, the 21d of last dispenser, statistics disease index and control efficiency.Every processing 60 strain cigarette seedlings, test repeats 3 times.Calculate preventive effect.The therapeutic action measurement result is as shown in table 5.
The therapeutic action measurement result of table 5:3 kind stone-like omphalia antivirotic to common cigarette
Results from pot experiment test shows (table 4, table 5); 3 kinds of stone-like omphalia antivirotics show good prevention and result for the treatment of to tobacco mosaic virus disease; its protection and result for the treatment of all are better than the contrast medicament, and wherein, 20% stone-like omphalia microemulsion relative control effect is higher than contrast medicament 10% left and right.
The drug effect test of field zone of embodiment 5:3 kind stone-like omphalia antivirotic to TMV
Small plot experiment is randomized arrangement, repeat 3 times, residential quarter area depending on actual conditions, but area can not be less than 30 ㎡, experimental field select to require fertility evenly, crop-planting is consistent with managerial skills, the state of an illness occur and the extent of injury more even; To establish guard row between each is processed and around the test region.Carrying out the blade face conventional spray, the moroxydine take 20% with 3 kinds of stone-like omphalia antivirotics 100,200,400 times of liquid. 500 times of liquid of second copper wetting powder carry out the blade face conventional spray as contrasting medicament, and establish the clear water contrast; All reagent agents must be carried out secondary dilution.The idiopathy initial stage begins to spray medicine, afterwards every 7d spray 1 time, totally 3 times.Spray for the first time before medicine and the 10d Investigate incidence after last spray medicine and statistics disease index, calculate preventive effect, the results are shown in Table 6.
Table 6:3 kind stone-like omphalia antivirotic control tobacco virus drug effect test of field zone
Annotate: in table, data are the mean value of 3 repetitions; In " preventive effect " hurdle, refer to be remarkable difference on α=0.05 level with different letters after the ordered series of numbers mark.As can be known from the above table, 3 kinds of stone-like omphalia antivirotics have good control efficiency to tobacco mosaic virus, obviously are better than the viral A of medicament contrast in the situation that dilute 100,200,400 times,
Claims (4)
1. a stone-like omphalia anti-plant virus agent, is characterized in that, this stone-like omphalia anti-plant virus agent that makes is take Omphalia extract as principal component and adds auxiliary agent to process, and the preparation form is soluble liquid, aqua and microemulsion; Wherein:
Described microemulsion is prepared by following raw materials by weight:
Omphalia extract: 10%～30%, organic solvent: 30%～60%, surfactant: 5%～30%, antifreezing agent: 4%～10%, surplus is water, the percentage by weight sum of raw material is 100%;
Described soluble liquid is prepared by following raw materials by weight:
Omphalia extract: 10%～30%, organic solvent: 30%～60%, surfactant: 5%～30%, the percentage by weight sum of raw material is 100%;
Described aqua is prepared by following raw materials by weight:
Omphalia extract: 10%～30%, organic solvent: 30%～60%, surfactant: 5%～30%, antifreezing agent: 4%～10%, surplus is water, the percentage by weight sum of raw material is 100%.
2. stone-like omphalia anti-plant virus agent as claimed in claim 1 is characterized in that:
Described organic solvent is one or more the mixture in ethyl acetate, cyclohexanone, methyl oleate, ethanol, methyl alcohol, acetone, dimethyl formamide;
Described emulsifier is one or more the mixture in alkyl benzene sulfonate, alkylphenol polyoxyethylene class, multi-styrene phenol APEO, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers and similar substance thereof, this Pan's series, tween, agricultural anion-phosphate;
Described surfactant is one or more mixture in the addition product, aralkyl phenol polyethenoxy ether formaldehyde condensation products of alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers and similar substance, castor oil and oxirane;
Described antifreezing agent is ethylene glycol or glycerine.
3. the preparation method of stone-like omphalia anti-plant virus agent claimed in claim 1, is characterized in that, adopts following steps:
A, the stone-like omphalia sclerotium is minced with the meal that the plant tissue cracker is crushed to 1～5mm.
B, the meal of steps A gained is minced adopt the solvent method lixiviate, extraction solvent is any one or a few in ethanol, ethyl acetate, chloroform, benzinum, water, extracting temperature is 50～80 ℃, extraction time is 6～12 hours, extract through Vacuum Concentration to being equivalent to 1kg dry powder/kg concentrate;
C, with the concentrate of step B gained, in the still that is mixed, the still temperature that is mixed is 35-60 ℃ according to formula ratio and organic solvent and surfactant, mixing time is 30 minutes, namely gets stone-like omphalia Antiphytoviral microemulsion after stirring;
D, with the concentrate of step B gained, in the still that is mixed, the still temperature that is mixed is 35 ℃～60 ℃ according to formula ratio and organic solvent, antifreezing agent and surfactant, mixing time is 30 minutes, namely gets stone-like omphalia Antiphytoviral soluble liquid after stirring;
E, with the concentrate of step B gained, be placed according to formula ratio and organic solvent, surfactant, antifreezing agent and water the still that is mixed, the still temperature that is mixed is 35-60 ℃, mixing time is 30 minutes, namely gets stone-like omphalia Antiphytoviral aqua after stirring.
4. method as claimed in claim 3 is characterized in that:
Described organic solvent is ethyl acetate, cyclohexanone, methyl oleate, ethanol, methyl alcohol, acetone, the mixture of one or more in dimethyl formamide;
Described emulsifier is one or more the mixture in alkyl benzene sulfonate, alkylphenol polyoxyethylene class, multi-styrene phenol APEO, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers, this Pan's series, tween, agricultural anion-phosphate;
Described surfactant is one or more mixture in the addition product, aralkyl phenol polyethenoxy ether formaldehyde condensation products of alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers, castor oil and oxirane;
Described antifreezing agent is ethylene glycol or glycerine.
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| CN111657305A (en) * | 2020-05-06 | 2020-09-15 | 西北农林科技大学 | Application of fennel extract in preparation of anti-plant virus agent and anti-plant virus agent |
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| CN111657305A (en) * | 2020-05-06 | 2020-09-15 | 西北农林科技大学 | Application of fennel extract in preparation of anti-plant virus agent and anti-plant virus agent |
| CN111657305B (en) * | 2020-05-06 | 2021-09-07 | 西北农林科技大学 | Application of fennel extract in preparation of anti-plant virus agent and anti-plant virus agent |
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Application publication date: 20130529 |