CN103120189A - Cephalotaxus sinensis Li. plant-virus-resisting agent and preparation method thereof - Google Patents
Cephalotaxus sinensis Li. plant-virus-resisting agent and preparation method thereof Download PDFInfo
- Publication number
- CN103120189A CN103120189A CN2013100484043A CN201310048404A CN103120189A CN 103120189 A CN103120189 A CN 103120189A CN 2013100484043 A CN2013100484043 A CN 2013100484043A CN 201310048404 A CN201310048404 A CN 201310048404A CN 103120189 A CN103120189 A CN 103120189A
- Authority
- CN
- China
- Prior art keywords
- virus
- caephalotaxus sinensis
- plant
- sinensis
- surfactant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a plant-derived virus-resisting agent which takes Cephalotaxus sinensis Li. extract as an active component and a preparation method thereof. The preparation method comprises the following steps: pulverizing stems and leaves of Cephalotaxus sinensis Li.; extracting; and through a certain processing course, preparing Cephalotaxus sinensis Li. virus-resisting soluble liquid, water and microemulsion which can be used for controlling tobacco mosaic virus (TMV) diseases, cucumber mosaic virus (CMV) diseases, potato virus X and Y (PVX and PVY) diseases and other multiple plant virus diseases on tobaccos, chilies, tomatoes, vegetable marrows, potatoes and other crops. The preparation contains 10-30% of Cephalotaxus sinensis Li. extract and the balance of assistant. The plant-virus-resisting agent disclosed by the invention has favorable virus-resisting effect, and is safe to the environment, humans, livestock, natural enemies of pests and other beneficial organisms. The preparation process of the plant-virus-resisting agent is simple, low in cost and suitable for popularization.
Description
Technical field
The invention belongs to plant and application thereof, be specifically related to caephalotaxus sinensis anti-plant viral disease preparation and preparation method thereof.
Background technology
Plant virus is a kind ofly to infect active cytozoicus pathogen by having of forming of nucleic acid and protein, and according to the 8th report of ICTV (ICTV), the plant virus kind found of the whole world reaches 1122 at present.The caused plant disease of plant virus have the title of " plant cancer ", and its extent of injury to crops is only second to the mycosis original, and almost every kind of crops all are subject to the harm of 2 ~ 3 kinds of viruses.Approximately 400 hundred million dollars of the losses that is caused by plant virus in whole world every year, only the harm that causes of tobacco mosaic virus (Tobacco mosaic virus, TMV) is just over 100,000,000 dollars.
Because plant virus has strict parasitism, it colonizes in and carries out copying of nucleic acid (RNA or DNA) and synthesizing of protein coat in plant cell or even cell nucleus, forms new virion.This difficulty of prevention and cure that has just determined the viroses of plant is large, and general medicament does not have effect.Have the inhibiting substances of TMV in 20 beginning of the century Allard report Phytolacca acinosa juice, the emphasis of preventing and treating afterwards plant virus turns to natural plant extract direction gradually.Particularly since the nineties in last century, people have found the inhibiting substances of many Antiphytovirals successively from natural products, provide the foundation for developing efficient, single-minded, safe plant virus natural inhibitor.
Cephalotaxus sinensis (Cephalotaxus sinensis Li.) is Cephalotaxaceae, and cephalotaxus plant has another name called caephalotaxus sinensis (Zhejiang), west place in Hubei caephalotaxus sinensis (Chinese tree systematics), Chinese caephalotaxus sinensis China fir, caephalotaxus sinensis China fir (Chinese gymnosperm will).Cephalotaxus sinensis is China's endemic tree, distribute very wide, originate in South of Jiangsu Province, Zhejiang, Southern Anhui Province, Fujian, Jiangxi, Henan, Hunan, Hubei, Southern Shaanxi, SOUTH OF GANSU, Sichuan, southeastern Yunnan, Northeastern Guizhou, Guangxi, Surface in Southwest Coastal Area of Guangdong, majority is born in granite, sandstone and the Limestone Mountain of height above sea level 600-2200 rice.Timber is solid, can be used as farm implements and technique etc.Leaf, branch, seed, root can extract various plants alkali, to treatment leukemia and lymphosarcoma etc. have certain curative effect (Chinese Plants will editorial board of the Chinese Academy of Sciences: " Chinese Plants will ", the 7th volume, Science Press, 1978 years, P.428-432).
About cephalotaxus sinensis and metabolite thereof, the effect of disease, worm, crop smothering in agricultural production only had fragmentary report at present.report cephalotaxus sinensis alcaloid-derivatives is arranged, and (suffering is kindly helped secure the success of to the better passivation of tobacco mosaic virus tool, Wang Yuanqing. the action effect preliminary study [J] of harringtonine synthetic to tobacco mosaic virus. the Laiyang Agricultural College journal, 1998, 15(3): 205-207.), and has an activity of weeding (Hao Shuanhong, Wei Yan, open green, separate and to the activity of weeding [J] of Amaranthus retroflexus Deng. cephalotaxus sinensis branches and leaves extract. the Pesticide Science journal, 2006, 8(1): 91-94.), cephalotaxus sinensis is to the stronger antifeedant activity (Zhang Xing of mythimna separata tool, Yang Chongzhen. the screening of Resources of Botanical Insecticides in Northwestern China [J]. the journal .1999 of Northwest Agricultural University, 27(2): 21-27), also has stronger eelworm-killing activity (Wen Yanhua, Feng Zhixin, Xu Hanhong etc., plant extract kills line screening active ingredients [J] to the several plant pathogenic nematode. the journal .2001 of Hua Zhong Agriculture University, 20(3): 235-238).But these are all measurement results in the laboratory, and so far there are no to the Antiphytoviral effect of its development system research, do not form product, can not be applied to agricultural production.
Summary of the invention
The object of the invention is to, take caephalotaxus sinensis (Cephalotaxus sinensis Li.) as raw material, provide high, nuisanceless, cheap plant resource anti-plant virus agent of a kind of Antiphytoviral effect and preparation method thereof.
In order to realize above-mentioned task, the present invention takes following technical solution:
A kind of caephalotaxus sinensis anti-plant virus agent is characterized in that, this caephalotaxus sinensis anti-plant virus agent that makes is take the caephalotaxus sinensis extract as principal component and add auxiliary agent to process, and the preparation form is soluble liquid, aqua and microemulsion; Wherein:
Described microemulsion is prepared by following raw materials by weight:
Caephalotaxus sinensis extract 10%～30%, organic solvent: 30%～60%, surfactant: 5%～20%, antifreezing agent: 5%～10%, surplus is water, the percentage by weight sum of raw material is 100%;
Described soluble liquid is prepared by following raw materials by weight:
Caephalotaxus sinensis extract 10%～30%, organic solvent: 30%～60%, surfactant: 5%～30%, the percentage by weight sum of raw material is 100%;
Described aqua is prepared by following raw materials by weight:
Caephalotaxus sinensis extract 10%～30%, organic solvent: 30%～60%, surfactant: 5%～30%, antifreezing agent: 5%～10%, surplus is water, the percentage by weight sum of raw material is 100%;
Above-mentioned organic solvent is ethyl acetate, cyclohexanone, methyl oleate, ethanol, methyl alcohol, acetone, the mixture of one or more in dimethyl formamide;
Described emulsifier is one or more the mixture in alkyl benzene sulfonate, alkylphenol polyoxyethylene class, multi-styrene phenol APEO, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers and similar substance thereof, this Pan's series, tween, agricultural anion-phosphate;
Described surfactant is one or more mixture in the addition product, aralkyl phenol polyethenoxy ether formaldehyde condensation products of alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers and similar substance, castor oil and oxirane;
Described antifreezing agent is ethylene glycol or glycerine.
The preparation method of above-mentioned caephalotaxus sinensis anti-plant virus agent is characterized in that, adopts following steps:
A, caephalotaxus sinensis is minced with the meal that the plant tissue cracker is crushed to 1～5mm.
B, the meal of steps A gained is minced adopt the solvent method lixiviate, extraction solvent is any one or a few in ethanol, ethyl acetate, chloroform, benzinum, water, extracting temperature is 50～80 ℃, extraction time is 18～20 hours, extract through Vacuum Concentration to being equivalent to 1kg dry powder/kg concentrate;
C, with the concentrate of step B gained, be placed according to formula ratio and organic solvent and surfactant the still that is mixed, namely get caephalotaxus sinensis Antiphytoviral microemulsion after stirring;
D, with the concentrate of step B gained, be placed according to formula ratio and organic solvent and surfactant the still that is mixed, namely get caephalotaxus sinensis Antiphytoviral soluble liquid after stirring;
E, with the concentrate of step B gained, be placed according to formula ratio and organic solvent, surfactant and water the still that is mixed, namely get caephalotaxus sinensis Antiphytoviral aqua after stirring.
The made caephalotaxus sinensis anti-plant virus agent of the present invention has the following advantages:
1. anti-phytoviral activity is remarkable, can be used for preventing and treating on tobacco, capsicum, tomato, custard squash, potatoes and other crops tobacco mosaic virus disease (Tobacco mosaic virus, TMV), Cucumber Mosaic Virus (Cucumber mosaic virus, CMV) and potato virus disease (Potato Virus X, PVX; Potato Virus Y, PVY) etc. the various plants virus disease;
2. to people, animal, pest natural enemy and other beneficial organism safety, environment compatibility is good;
3. biogenic pesticide, use rear noresidue to poison;
4. simple, with low cost, suitable the promoting the use of of aboundresources, preparation method.
Embodiment
In order better to understand essence of an invention, the below describes the technology contents of invention in detail with embodiment, but invention is not limited to these embodiment.
Plant antiviral agent of the present invention take the caephalotaxus sinensis extract as active substance, adds the certain proportion auxiliary agent to carry out formulation, can make environment-friendly type preparation microemulsion, aqua or soluble liquid.
Microemulsion is prepared by following raw materials by weight:
The caephalotaxus sinensis extract: 10%～30%, organic solvent: 30%～60%, surfactant: 5%～20%, antifreezing agent: 5%～10%, surplus is water, the percentage by weight sum of raw material is 100%.
Soluble liquid is prepared by following raw materials by weight:
The caephalotaxus sinensis extract: 10%～30%, organic solvent: 30%～60%, surfactant: 5%～30%, the percentage by weight sum of raw material is 100%;
Aqua is prepared by following raw materials by weight:
The caephalotaxus sinensis extract: 10%～30%, organic solvent: 30%～60%, surfactant: 5%～30%, antifreezing agent: 5%～10%, surplus is water, the percentage by weight sum of raw material is 100%.
Described organic solvent is one or more the mixture in ethyl acetate, cyclohexanone, methyl oleate, ethanol, methyl alcohol, acetone, dimethyl formamide;
Described emulsifier is one or more the mixture in alkyl benzene sulfonate, alkylphenol polyoxyethylene class, multi-styrene phenol APEO, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers, this Pan's series, tween, agricultural anion-phosphate;
Described surfactant is one or more mixture in the addition product, aralkyl phenol polyethenoxy ether formaldehyde condensation products of alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers, castor oil and oxirane;
Described antifreezing agent is ethylene glycol or glycerine.
The preparation method of caephalotaxus sinensis anti-plant virus agent, adopt following steps:
1, the acquisition of caephalotaxus sinensis extract:
A, caephalotaxus sinensis is minced with the meal that the plant tissue cracker is crushed to 1～5mm.
B, the meal of steps A gained is minced adopt conventional solvent method lixiviate, extraction solvent is any one or a few in ethanol, ethyl acetate, chloroform, benzinum, water, extracting temperature is 50～80 ℃, extraction time is 18～20 hours, extract to being equivalent to 1kg dry powder/kg concentrate, is the caephalotaxus sinensis extract through Vacuum Concentration.
2, caephalotaxus sinensis anti-plant virus agent preparation
1) with the concentrate of step B gained, be placed according to formula ratio and organic solvent, antifreezing agent and surfactant the still that is mixed, namely get caephalotaxus sinensis Antiphytoviral microemulsion after stirring.
2) with the concentrate of step B gained, be placed according to formula ratio and organic solvent and surfactant the still that is mixed, namely get caephalotaxus sinensis Antiphytoviral soluble liquid after stirring.
3) with the concentrate of step B gained, be placed according to formula ratio and organic solvent, surfactant, antifreezing agent and water the still that is mixed, namely get caephalotaxus sinensis Antiphytoviral aqua after stirring.
Through inventor's evidence, 20% caephalotaxus sinensis microemulsion, 20% caephalotaxus sinensis soluble liquid and 10% caephalotaxus sinensis aqua have good control efficiency to tobacco mosaic virus (TMV).
Below the embodiment that the inventor provides.
The preparation of embodiment 1:20% caephalotaxus sinensis microemulsion
Take caephalotaxus sinensis concentrate 20kg, be dissolved in 30kg ethyl acetate, then add the 15kg calcium dodecyl benzene sulfonate, 5kg styrene acid APEO, 5kg ethylene glycol, mixed under high-speed stirred, be heated to 40 ℃ of left and right; Drip deionized water 30kg under high-speed stirred, control the rate of addition of water, make temperature remain on 40 ℃ of left and right; Water dropwises, and is warming up to 50 ℃, stirs 1 hour, makes 20% caephalotaxus sinensis microemulsion 100kg.The stability of preparation, outward appearance etc. meet the requirement of commodity preparation.
The preparation of embodiment 2:20% caephalotaxus sinensis soluble liquid
Take caephalotaxus sinensis concentrate 20kg, be dissolved in 30kg ethyl acetate, then add the 5kg alkylphenol polyoxyethylene, 1kg APG A, the ethanol polishing is to 100kg, after mixing, stirred 10～30 minutes with 800～1000 rev/mins of mixing speeds, can make caephalotaxus sinensis soluble liquid 100kg.The stability of preparation, outward appearance etc. meet the requirement of commodity preparation.
The preparation of embodiment 3:10% caephalotaxus sinensis aqua
Take caephalotaxus sinensis concentrate 10kg, be dissolved in 30kg ethyl acetate, add again the 5kg alkylphenol polyoxyethylene, 1kg APG A, 5kg ethylene glycol, the water polishing is to 100kg, after mixing, stirred 10～30 minutes with 800～1000 rev/mins of mixing speeds, can make caephalotaxus sinensis soluble liquid 100kg.The stability of preparation, outward appearance etc. meet the requirement of commodity preparation.
The indoor bioassay of embodiment 4:3 kind caephalotaxus sinensis antivirotic to TMV
(1) to the external passivation effect of TMV
Adopt the half withered spot method of leaf to measure, choose 5～6 leaf phase Nicotiana glutinosas (Nicotiana glutinosa) that growth is vigorous, growing way is consistent and be withered spot host.Take vein as the boundary, Zuo Banye inoculation liquid and viral equal-volume mixed liquor, right half leaf inoculation distilled water and viral equal-volume mixed liquor compare, and virus inoculation concentration is 10 μ g/mL, and passivation time is 5min, rinses the inoculation blade surface with clear water immediately after inoculation.4 leaves of every processing inoculation, test repeats 3 times, adds up withered spot number after 3d, calculates inhibiting rate.
Inhibiting rate (%)=(contrast withered spot number-processings withered spot number)/contrast withered spot number * 100
Measurement result sees Table 1.
Table 1:3 kind caephalotaxus sinensis antivirotic is to the TMV inactivation in vitro
(2) inhibition that TMV is just infected
Adopt the half withered spot method of leaf, choosing 5～6 consistent, healthy leaf phase Nicotiana glutinosas of growing way is withered spot host, virus inoculation after smearing liquid 6h, 12h, 24h, blank is that clear water is processed, and each processes 4 leaves of inoculation, repeats 3 times, add up withered spot number after 3d, calculate inhibiting rate.The results are shown in Table 2.
The inhibition that table 2:3 kind caephalotaxus sinensis antivirotic just infects TMV
The inhibition of (3) TMV being bred
Adopt the half withered spot method of leaf, choosing 5～6 consistent, healthy leaf phase Nicotiana glutinosas of growing way is withered spot host, smears liquid after virus inoculation 6h, 12h, 24h, blank is that clear water is processed, and each processes 4 leaves of inoculation, repeats 3 times, add up withered spot number after 3d, calculate inhibiting rate.The results are shown in Table 3.
The inhibition of table 3:3 kind caephalotaxus sinensis antivirotic to TMV propagation
By table 1,2,3 as can be known, the passivation to TMV of 3 kinds of caephalotaxus sinensis antivirotics is just infected, proliferation inhibiting effect is good, apparently higher than contrast medicament moroxydine. second copper.
The pot experiment of embodiment 4:3 kind caephalotaxus sinensis antivirotic to TMV
(1) to the protective effect of common cigarette
Select the 5-6 leaf phase, consistent, the healthy and strong common cigarette (Nicotiana tabacum) of growing way supplies examination.2 groups of prevention group (dispenser 3 times then inoculate TMV) and control groups (only inoculating not dispenser) are established in test.With 200 times of liquid sprayings of 3 kinds of caephalotaxus sinensis antivirotics, contrast medicament moroxydine. common cigarette is processed in 500 times of liquid sprayings of second copper, and every 5d dispenser once is total to dispenser three times.Inoculate TMV after last dispenser 24h, inoculum density is 1:20 (W/V).After inoculation, 14d, 21d check incidence, statistics disease index and control efficiency.Every processing 60 strain cigarette seedlings, test repeats 3 times.Calculate preventive effect.
The incidence of disease=(the total strain number of diseased plant number/processing of getting a haircut everywhere) * 100%
Disease index=∑ (sick progression * diseased plant number)/(the highest sick level * respectively process total strain number) * 100
Before disease index growth rate=(after the spray medicine, disease refers to-spray that disease refers to before medicine)/spray medicine disease refer to * 100%
Control efficiency=(the contrast disease refers to-process that disease refers to)/contrast disease to refer to * 100%
The protective effect measurement result is as shown in table 4.
The protective effect measurement result of table 43 kind of caephalotaxus sinensis antivirotic to common cigarette
(2) to the therapeutic action of common cigarette
Select the 5-6 leaf phase, consistent, the healthy and strong common cigarette (Nicotiana tabacum) of growing way supplies examination.2 groups for the treatment of group (inoculation TMV after dispenser 3 times) and control groups (only inoculating not dispenser) are established in test.Inoculate malicious source TMV, inoculum density is 1:20 (W/V), and after inoculation, every 5d dispenser once, is total to dispenser three times.Medicament is 200 times of liquid sprayings of 3 kinds of caephalotaxus sinensis antivirotics, contrast medicament moroxydine. 500 times of liquid sprayings of second copper.Check incidence during respectively at 14d, the 21d of last dispenser, statistics disease index and control efficiency.Every processing 60 strain cigarette seedlings, test repeats 3 times.Calculate preventive effect.The therapeutic action measurement result is as shown in table 5.
The therapeutic action measurement result of table 53 kind of caephalotaxus sinensis antivirotic to common cigarette
Results from pot experiment test shows (table 4, table 5); 3 kinds of caephalotaxus sinensis antivirotics show good prevention and result for the treatment of to tobacco mosaic virus disease; its protection and result for the treatment of all are better than the contrast medicament, and wherein, 20% caephalotaxus sinensis microemulsion protective effect relative control effect is higher than contrast medicament 20% left and right.
The drug effect test of field zone of embodiment 5:3 kind caephalotaxus sinensis antivirotic to TMV
Small plot experiment is randomized arrangement, repeats 3 times, and residential quarter area depending on actual conditions, but area can not be less than 30m
2, experimental field select to require fertility evenly, crop-planting is consistent with managerial skills, the state of an illness occur and the extent of injury more even; To establish guard row between each is processed and around the test region.Carrying out the blade face conventional spray, the moroxydine take 20% with 3 kinds of caephalotaxus sinensis antivirotics 100,200,400 times of liquid. 500 times of liquid of second copper wetting powder carry out the blade face conventional spray as contrasting medicament, and establish the clear water contrast; All reagent agents must be carried out secondary dilution.The idiopathy initial stage begins to spray medicine, afterwards every 7d spray 1 time, totally 3 times.Spray for the first time before medicine and the 10d Investigate incidence after last spray medicine and statistics disease index, calculate preventive effect, the results are shown in Table 6.
Table 63 kind of caephalotaxus sinensis antivirotic control tobacco virus drug effect test of field zone
Annotate: in table, data are the mean value of 3 repetitions; In " preventive effect " hurdle, refer to be remarkable difference on α=0.05 level with different letters after the ordered series of numbers mark.
As can be known from the above table, 3 kinds of caephalotaxus sinensis antivirotics have good control efficiency to tobacco mosaic virus, obviously are better than the viral A of medicament contrast, preventive effect significant difference in the situation that dilute 100,200,400 times.
Claims (4)
1. a caephalotaxus sinensis anti-plant virus agent, is characterized in that, this caephalotaxus sinensis anti-plant virus agent that makes is take the caephalotaxus sinensis extract as principal component and add auxiliary agent to process, and the preparation form is soluble liquid, aqua and microemulsion; Wherein:
Described microemulsion is prepared by following raw materials by weight:
Caephalotaxus sinensis extract 10%～30%, organic solvent: 30%～60%, surfactant: 5%～20%, antifreezing agent: 5%～10%, surplus is water, the percentage by weight sum of raw material is 100%;
Described soluble liquid is prepared by following raw materials by weight:
Caephalotaxus sinensis extract 10%～30%, organic solvent: 30%～60%, surfactant: 5%～30%, the percentage by weight sum of raw material is 100%;
Described aqua is prepared by following raw materials by weight:
Caephalotaxus sinensis extract 10%～30%, organic solvent: 30%～60%, surfactant: 5%～30%, antifreezing agent: 5%～10%, surplus is water, the percentage by weight sum of raw material is 100%.
2. caephalotaxus sinensis anti-plant virus agent as claimed in claim 1 is characterized in that:
Described organic solvent is one or more the mixture in ethyl acetate, cyclohexanone, methyl oleate, ethanol, methyl alcohol, acetone, dimethyl formamide;
Described emulsifier is one or more the mixture in alkyl benzene sulfonate, alkylphenol polyoxyethylene class, multi-styrene phenol APEO, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers, this Pan's series, tween, agricultural anion-phosphate;
Described surfactant is one or more mixture in the addition product, aralkyl phenol polyethenoxy ether formaldehyde condensation products of alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers, castor oil and oxirane;
Described antifreezing agent is ethylene glycol or glycerine.
3. the preparation method of caephalotaxus sinensis anti-plant virus agent claimed in claim 1, is characterized in that, adopts following steps:
A, caephalotaxus sinensis is minced with the meal that the plant tissue cracker is crushed to 1～5mm.
B, the meal of steps A gained is minced adopt the solvent method lixiviate, extraction solvent is any one or a few in ethanol, ethyl acetate, chloroform, benzinum, water, extracting temperature is 50～80 ℃, extraction time is 18～20 hours, extract through Vacuum Concentration to being equivalent to 1kg dry powder/kg concentrate;
C, with the concentrate of step B gained, be placed according to formula ratio and organic solvent and surfactant the still that is mixed, namely get caephalotaxus sinensis Antiphytoviral microemulsion after stirring;
D, with the concentrate of step B gained, be placed according to formula ratio and organic solvent and surfactant the still that is mixed, namely get caephalotaxus sinensis Antiphytoviral soluble liquid after stirring;
E, with the concentrate of step B gained, be placed according to formula ratio and organic solvent, surfactant and water the still that is mixed, namely get caephalotaxus sinensis Antiphytoviral aqua after stirring.
4. method as claimed in claim 3 is characterized in that:
Described organic solvent is ethyl acetate, the mixture of one or more in cyclohexanone, methyl oleate, ethanol, methyl alcohol, acetone, dimethyl formamide;
Described emulsifier is one or more the mixture in alkyl benzene sulfonate, alkylphenol polyoxyethylene class, multi-styrene phenol APEO, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers, this Pan's series, tween, agricultural anion-phosphate;
Described surfactant is one or more mixture in the addition product, aralkyl phenol polyethenoxy ether formaldehyde condensation products of alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers, castor oil and oxirane;
Described antifreezing agent is ethylene glycol or glycerine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013100484043A CN103120189A (en) | 2013-02-06 | 2013-02-06 | Cephalotaxus sinensis Li. plant-virus-resisting agent and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013100484043A CN103120189A (en) | 2013-02-06 | 2013-02-06 | Cephalotaxus sinensis Li. plant-virus-resisting agent and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103120189A true CN103120189A (en) | 2013-05-29 |
Family
ID=48451487
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013100484043A Pending CN103120189A (en) | 2013-02-06 | 2013-02-06 | Cephalotaxus sinensis Li. plant-virus-resisting agent and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103120189A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108477225A (en) * | 2018-05-03 | 2018-09-04 | 赖进九 | Composition pesticide containing metaflumizone and cephalotaxus alkaloids |
| CN114982790A (en) * | 2022-07-19 | 2022-09-02 | 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) | Microemulsion containing tobacco cis-abienol and application thereof |
| CN115251053A (en) * | 2022-07-19 | 2022-11-01 | 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) | Sclareol plant antiviral preparation and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1739583A (en) * | 2005-06-21 | 2006-03-01 | 北京理工大学 | Prepn process and application of Chinese medicine prepn containing Chinese torreya nut |
| CN101629193A (en) * | 2009-08-14 | 2010-01-20 | 江苏大学 | Method for producing anti-cancer alkaloid by culturing cephalotaxus hainanensis li cells |
-
2013
- 2013-02-06 CN CN2013100484043A patent/CN103120189A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1739583A (en) * | 2005-06-21 | 2006-03-01 | 北京理工大学 | Prepn process and application of Chinese medicine prepn containing Chinese torreya nut |
| CN101629193A (en) * | 2009-08-14 | 2010-01-20 | 江苏大学 | Method for producing anti-cancer alkaloid by culturing cephalotaxus hainanensis li cells |
Non-Patent Citations (1)
| Title |
|---|
| 景炳年: "植物源病毒抑制剂VFB活性成分研究及抗TMV植物样品筛选", 《中国博士学位论文全文数据库（电子期刊）》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108477225A (en) * | 2018-05-03 | 2018-09-04 | 赖进九 | Composition pesticide containing metaflumizone and cephalotaxus alkaloids |
| CN114982790A (en) * | 2022-07-19 | 2022-09-02 | 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) | Microemulsion containing tobacco cis-abienol and application thereof |
| CN115251053A (en) * | 2022-07-19 | 2022-11-01 | 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) | Sclareol plant antiviral preparation and application thereof |
| CN115251053B (en) * | 2022-07-19 | 2023-09-26 | 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) | Sclareol plant antiviral preparation and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104738102B (en) | Chinese gooseberry root bark extract is used for the application for preparing anti-plant virus agent | |
| CN103766414B (en) | A kind of containing emblic plant resource resisting tobacco mosaic virus agent and preparation method thereof | |
| CN104430604A (en) | Oxymatrine emulsifiable concentrate pesticide | |
| CN103155953A (en) | Pomegranate peel polyphenol plant fungicide and application and preparation method | |
| CN106259575A (en) | A kind of plant liquid extract and plant protecting liquid | |
| CN103120189A (en) | Cephalotaxus sinensis Li. plant-virus-resisting agent and preparation method thereof | |
| CN103238637B (en) | Botanical pesticide for preventing and treating potato ring rot and preparation method thereof | |
| CN104146012B (en) | Preparation of control vegetable seedling virus disease and its preparation method and application | |
| CN103749490B (en) | Plant virus resistant agent and preparation method thereof | |
| CN102265906A (en) | Artemisia ordosica Kraschen bactericide and preparation method thereof | |
| CN101467531A (en) | Pepper element pesticide and preparation method thereof | |
| CN103141525A (en) | Rhodiola eurycarpa anti-plant virus agent and preparation method thereof | |
| CN102318649B (en) | Plant-derived preparation against plant viruses | |
| CN103704284A (en) | Pesticide composition containing black false hellebore and acorus calamus extracts as well as preparation method and application | |
| CN103734204A (en) | Pesticide composite containing schizonepeta and tobacco extract, preparation method and application | |
| CN103141524B (en) | The agent of a kind of Chinese Abelia anti cucumber mosaic virus | |
| CN103689002B (en) | Containing composition pesticide and the preparation method and application of lantana and Azolla imbricata extract | |
| CN103120188A (en) | Omphalia lapidescens Schroet plant-virus-resisting agent and preparation method thereof | |
| CN103651592B (en) | Containing composition pesticide and the preparation method and application of Dalmatian chrysanthemum and extract from Lantana camara | |
| CN106259515A (en) | A kind of mango insecticide and preparation method thereof | |
| CN112586514A (en) | Insect-resistant plant preparation and method for preventing and treating red spiders by using same | |
| CN105594763A (en) | Traditional Chinese medicine composition for preventing and treating radix scutellariae leaf blight and preparation method thereof | |
| Kwak et al. | First report of tomato aspermy virus in Chrysanthemum zawadskii var. latilobum in Korea | |
| CN105284915A (en) | Pesticide composition containing extracts of folium isatidis and thyme and preparation method and application thereof | |
| CN105248481A (en) | Aphid control liquid for facility crops and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20130529 |