CN104412972B - Trans-ferulaic acid microemulsion and preparation method thereof and the application preventing and treating Nicotiana tabacum L., Oryza sativa L., tomato virus disease - Google Patents
Trans-ferulaic acid microemulsion and preparation method thereof and the application preventing and treating Nicotiana tabacum L., Oryza sativa L., tomato virus disease Download PDFInfo
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Abstract
The present invention relates to trans-ferulaic acid microemulsion and preparation method thereof and prevent and treat the application of Nicotiana tabacum L., Oryza sativa L., tomato virus disease.Trans-ferulaic acid, dimethylformamide, Ketohexamethylene, methanol are added together, after fully dissolving, add emulsifying agent, mix homogeneously, drip water while stirring, obtain trans-ferulaic acid microemulsion.Field test results shows: trans-ferulaic acid microemulsion can well prevent and treat Nicotiana tabacum L., Oryza sativa L., tomato virus disease, to trial crops safety.Trans-ferulaic acid microemulsion has dosage province, lasting period length, cause environmental pollution little owing to drastically reduce the area the use of volatile organic solvent and manufacturing cost is low, to advantages such as crop safeties.
Description
Technical field
The present invention relates to trans-ferulaic acid microemulsion and preparation method thereof and prevent and treat the application of Nicotiana tabacum L., Oryza sativa L., tomato virus disease.
Background technology
The viroses of plant are very serious plant diseases, prevent and treat extremely difficult, have the title of " plant cancer ".The eruption and prevalence of viral diseases of plants is very extensive, and disaster is very serious.Nearly all vegetable, cereal crops, fruit tree etc. all infect virosis, make agricultural production be subject to huge loss, reduce product quality, seriously promoted the generation of other physiological disturbance of plant.Such as the most serious popular tobacco mosaic virus (TMV) (Tobaccomosaicvirus, TMV) it is a kind of very strong, virus that harm is very wide stable, infectious, its host is very extensive, the object of infringement has tens kinds, such as Nicotiana tabacum L., Fructus Lycopersici esculenti, Fructus Capsici and brassicaceous vegetable, Chenopodiaceae, Amaranthaceae, Cucurbitaceae, Aizoaceae, pulse family and Phytolaccaceae etc..According to public goods center, whole nation document announcement, China is nearly ten years, annual Virus Diseases of Rice hazard area is at about 30,000,000 mu, within 2010, China's Virus Diseases of Rice is in seriously breaking out trend, particularly sick (the southernriceblackstripeddwarfvirus of southern rice black-streaked dwarf, SRBSDV), late rice population outbreak in 9 provinces (autonomous region) such as Guangdong, Hunan, Jiangxi, Guangxi, Oryza sativa L. injured area more than 20,000,000 mu, the production loss that China causes because of Virus Diseases of Rice is 30%, and economic loss is up to 10,000,000,000 yuan.
The practical kind of effective and gratifying plant virus inhibitor is little at present, and especially the therapeutic medicament of specially good effect is less, and wherein the preventive effect of Ningnanmycin is best.Conventional has three below commercial varieties: Ningnanmycin, field using dosage is 100gai/hm2, it being 50%～60% to tobacco mosaic disease prevention effect, have good preventive effect, therapeutic effect is not good；Virazole, field using dosage is 600gai/hm2, the preventive effect to plant virus (tobacco mosaic virus (TMV), cucumber mosaic virus, potato virus X, marmor upsilon etc.) is 30～60%；Virus A, field using dosage is 600gai/hm2, it is 50% to tobacco mosaic disease prevention effect.Guizhou University professor Song Baoan has formulated viral star, and field using dosage is 300gai/hm2.Therefore, the needs of efficient, safe Control of Plant Virus Disease medicament are extremely urgent.
Botanical pesticide, as real public nuisance-free agricultural chemicals, has the advantage that (1) has its degradation pathway smoothly at nature, and environment is excellent good；(2) model of action is unique, it is more difficult to develop immunity to drugs；(3) they generally have selectivity by force, and people, animal and natural enemy toxicity are low；(4) botanical pesticide is generally water preparation, easily decomposes after the effect by sunlight or microorganism, and the half-life is short, and residue degrading is fast, is taken food rear enrichment mechanism by animal poor, will not produce poisoning.Therefore, botanical pesticide is listed in and is exempted from residue limits list by European Union.
Trans-ferulaic acid is a kind of phenolic acid being widely present in plant, plant cell wall cross-links, with polysaccharide and lignin, the part constituting cell wall, it is one of the effective elements of the medicine in Resina Ferulae, Radix Angelicae Sinensis, Rhizoma Chuanxiong, Rhizoma Cimicifugae etc., is applied to medicine, health product, cosmetic material and food additive aspect because it has stronger antioxidant activity and antisepsis.In recent years the research extensively and profoundly of its pharmacological effect aspect being found, ferulic acid and its derivatives also has good antipyretic-antalgic, antithrombotic, blood fat reducing, anti-inflammation, anti-cancer, mutation, enhancing immunologic function and strengthens the biological activity such as human sperm vigor and mobility, its toxicity is extremely low, almost without side effect.At present, except ferulic acid as food, grain preservative except related application agriculturally, agriculturally can belong to blank for the research of anti-plant viral disease activity is real about ferulic acid and its derivatives.We find trans-ferulaic acid (test code number NK-11 first, structural formula is as follows) tobacco mosaic virus (TMV) can be suppressed well, can effectively prevent and treat the tobacco mosaic virus disease (CN102090412A) of Nicotiana tabacum L., Fructus Capsici, Fructus Lycopersici esculenti, melon dish, grain, vegetable, beans various crop.
Trans-ferulaic acid microemulsion and preparation method thereof and preventing and treating Nicotiana tabacum L., Oryza sativa L., tomato virus disease the patent of application but without application.
Summary of the invention
An object of the present invention is in that to provide a kind of trans-ferulaic acid microemulsion, and its trans-ferulaic acid microemulsion is the one of the agricultural dosage form of trans-ferulaic acid.There is dosage province, lasting period length, cause environmental pollution little owing to drastically reduce the area the use of volatile organic solvent and manufacturing cost is low, to advantages such as crop safeties.
The two of the purpose of the present invention are in that the preparation method providing above-mentioned trans-ferulaic acid microemulsion.
The three of the purpose of the present invention are in that to provide above-mentioned trans-ferulaic acid microemulsion for preventing and treating Nicotiana tabacum L., Oryza sativa L., tomato virus disease.
Trans-ferulaic acid microemulsion as first aspect present invention, it is characterised in that effective ingredient is trans-ferulaic acid, dosage form is microemulsion.The weight of trans-ferulaic acid shared mass percent in described trans-ferulaic acid microemulsion is 2%-20%.
Trans-ferulaic acid microemulsion of the present invention, is made up of trans-ferulaic acid, solvent, emulsifying agent, water.
Described solvent is preferably dimethylformamide, Ketohexamethylene, methanol three kinds mixing.
Described emulsifying agent is preferably EL-40 (i.e. Oleum Ricini ethylene oxide adduct or condensation substance), OP-10 (i.e. OPEO), agriculture breast 2201.
Described water is preferably distilled water.
Trans-ferulaic acid microemulsion preparation method of the present invention, is trans-ferulaic acid, dimethylformamide, Ketohexamethylene, methanol are added together, and after fully dissolving, adds emulsifying agent, mix homogeneously, drips water while stirring, obtain trans-ferulaic acid microemulsion.
Trans-ferulaic acid microemulsion of the present invention is used for preventing and treating Nicotiana tabacum L., Oryza sativa L., tomato virus disease, tobacco virus can effectively be prevented and treated by 5% trans-ferulaic acid microemulsion, when active ingredient makes consumption be 600 grams/ha, the prevention effect of tobacco virus is better than comparison medicament 20% virus A wettable powder (active ingredient makes consumption be 600 grams/ha) and 2% Ningnanmycin water preparation (active ingredient makes consumption be 100 grams/ha), to trial crops safety；At rice seedling bed seeding stage, 5% trans-ferulaic acid microemulsion is 77.18% in 600 grams/ha of preventive effects to black streaked dwarf virus of rice of active ingredient amount, suitable in the preventive effect (77.22%) of active ingredient amount 100 grams/ha with 2% Ningnanmycin water preparation, at Oryza sativa L. Adult plant, Seedling Quality in Rice, growth to the disease index preventive effect of black streaked dwarf virus of rice, clump preventive effect, strain preventive effect respectively 65.29%, 70.96%, 54.89%, are had no adverse effects by 5% trans-ferulaic acid microemulsion active ingredient amount 600 grams/ha；5% trans-ferulaic acid microemulsion is in active ingredient amount 600 grams/ha, 300 grams/ha and 100 grams/ha of preventive effects to tomato virus disease respectively 57.22%, 52.22%, 48.33%, and compareing medicament 2% Ningnanmycin water preparation in preventive effect respectively 53.89% and 47.78% active ingredient amount 600 grams/ha of active ingredient amount 100 grams/ha and 20% virus A wettable powder, 5% trans-ferulaic acid microemulsion uses Fructus Lycopersici esculenti without poisoning at tomato growth stage.
Detailed description of the invention
In order to make the purpose of the present invention, technical scheme and advantage definitely, below in conjunction with embodiment, the present invention is described in further detail, is described herein as being only limitted to better explain and is not intended to limit the present invention the present invention.Embodiment is the formula that technical staff provides part trans-ferulaic acid microemulsion, and technical staff is referred to these formula and prepares.
The compound method of embodiment 1:5% trans-ferulaic acid microemulsion:
Trans-ferulaic acid 5% following for percentage by weight, dimethylformamide 27%, Ketohexamethylene 3%, methanol 30% are added together, after fully dissolving, add the Emulsifier EL-40 of percentage by weight 10%, mix homogeneously, drip the water of percentage by weight 25% while stirring, obtain 5% trans-ferulaic acid microemulsion.
The heat storage stability test of embodiment 2:5% trans-ferulaic acid microemulsion:
By 5% trans-ferulaic acid microemulsion in 54 ± 1 DEG C of heat storages two weeks, resolution ratio is less than 2%, and this heat storage stability describing 5% trans-ferulaic acid microemulsion is qualified.
The field control effectiveness test of embodiment 3:5% trans-ferulaic acid microemulsion preventing and treating tobacco virus:
Test arrangement is in Fu Jiaying village, Yuxi Jiangchuan County, Yunnan Province.Trial crops is Nicotiana tabacum L., and kind is Yun yan85.Controlling object: tobacco mosaic virus disease.Time: 2012.Result is in Table 1.
The field control effectiveness test result of table 15% trans-ferulaic acid microemulsion preventing and treating tobacco virus
Conclusion: tobacco virus can effectively be prevented and treated by 5% trans-ferulaic acid microemulsion, is better than comparison medicament 20% virus A wettable powder (active ingredient makes consumption be 600 grams/ha) and 2% Ningnanmycin water preparation (active ingredient makes consumption be 100 grams/ha) when active ingredient makes consumption be 600 grams/ha to the prevention effect of tobacco virus.Therefore, it is suggested that 5% trans-ferulaic acid microemulsion can research and develop the new medicament into preventing and treating tobacco virus, adopting nebulization dispenser at virosis their early stage, dispenser interval is 5～7 days, spraying times 2～3 times.Test medicine in above experimental concentration to trial crops safety.
The field control effectiveness test of black streaked dwarf virus of rice prevented and treated by embodiment 4:5% trans-ferulaic acid microemulsion:
Test arrangement is in China Paddy Rice Inst of Zhejiang Hangzhou Fuyang City proving ground.Trial crops is Oryza sativa L., and kind is elegant water 09.Controlling object: black streaked dwarf virus of rice.Time: 2012.Result is in Table 2-5.
Table 25% trans-ferulaic acid microemulsion is to Rice Seedlings black-streaked dwarf diseased plant preventive effect
Table 35% trans-ferulaic acid microemulsion is to land for growing field crops Adult plant black streaked dwarf virus of rice disease index preventive effect
Table 45% trans-ferulaic acid microemulsion is to land for growing field crops Adult plant black streaked dwarf virus of rice clump preventive effect
Table 55% trans-ferulaic acid microemulsion is to land for growing field crops Adult plant black streaked dwarf virus of rice strain preventive effect
Conclusion: at rice seedling bed seeding stage, 5% trans-ferulaic acid microemulsion is 77.18% in 600 grams/ha of preventive effects to black streaked dwarf virus of rice of active ingredient amount, suitable in the preventive effect (77.22%) of active ingredient amount 100 grams/ha with 2% Ningnanmycin water preparation.At Oryza sativa L. Adult plant, 5% trans-ferulaic acid microemulsion is in the active ingredient amount 600 grams/ha disease index preventive effect to black streaked dwarf virus of rice, clump preventive effect, strain preventive effect respectively 65.29%, 70.96%, 54.89%, 2% Ningnanmycin water preparation is in the active ingredient amount 100 grams/ha disease index preventive effect to black streaked dwarf virus of rice, clump preventive effect, strain preventive effect respectively 70.38%, 55.81%, 63.93%, 20% virus A wettable powder is in the active ingredient amount 600 grams/ha disease index preventive effect to black streaked dwarf virus of rice, clump preventive effect, strain preventive effect respectively 65.01%, 53.22%, 60.35%.Process on request and Seedling Quality in Rice, growth are had no adverse effects.
The field control effectiveness test of embodiment 5:5% trans-ferulaic acid microemulsion preventing and treating tomato virus disease:
Test arrangement is in proving ground, Tianjin Plant Protection Institute Wuqing.Trial crops is Fructus Lycopersici esculenti, and kind is that Semen Ginkgo is rich by force.Controlling object: tomato virus disease.Time: 2012.Result is in Table 6.
The field control effectiveness test effect of table 65% trans-ferulaic acid microemulsion preventing and treating tomato virus disease
Conclusion: 5% trans-ferulaic acid microemulsion is in active ingredient amount 600 grams/ha, 300 grams/ha and 100 grams/ha of preventive effects to tomato virus disease respectively 57.22%, 52.22%, 48.33%；Comparison medicament 2% Ningnanmycin water preparation is in active ingredient amount 100 grams/ha and the 20% viral A wettable powder preventive effect respectively 53.89% and 47.78% active ingredient amount 600 grams/ha.Test medicine 5% trans-ferulaic acid microemulsion uses Fructus Lycopersici esculenti without poisoning at tomato growth stage.
Claims (7)
1. trans-ferulaic acid microemulsion, it is characterized in that described microemulsion is made up of trans-ferulaic acid, solvent, emulsifying agent, water, trans-ferulaic acid mass percent in microemulsion is 2%-20%, described solvent is the mixing of dimethylformamide, Ketohexamethylene, methanol three kinds, and described emulsifying agent is Oleum Ricini ethylene oxide condensate EL-40, OPEO OP-10 or agriculture breast 2201.
2. trans-ferulaic acid microemulsion according to claim 1, it is characterised in that described water is distilled water.
3. the method for the trans-ferulaic acid microemulsion that a kind is prepared described in claim 1, it is characterised in that be trans-ferulaic acid, dimethylformamide, Ketohexamethylene, methanol are added together, after fully dissolving, add emulsifying agent, mix homogeneously, drips water while stirring, obtains trans-ferulaic acid microemulsion.
4. the application in preventing and treating tobacco virus of the trans-ferulaic acid microemulsion described in claim 1.
5. the application in preventing and treating Virus Diseases of Rice of the trans-ferulaic acid microemulsion described in claim 1.
6. application according to claim 5, it is characterised in that prevent and treat black streaked dwarf virus of rice at rice seedling bed seeding stage and Oryza sativa L. Adult plant.
7. the application in preventing and treating tomato virus disease of the trans-ferulaic acid microemulsion described in claim 1.
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| CN108990987A (en) * | 2018-08-03 | 2018-12-14 | 北京清源保生物科技有限公司 | The application of trans-ferulaic acid and phosphatide analog assistant in the prevention and treatment viroses of plant |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1988002602A1 (en) * | 1986-10-08 | 1988-04-21 | Dumbroff Erwin B | Control of senescence in fruits, vegetables and flowers |
| CN102090412A (en) * | 2009-12-11 | 2011-06-15 | 南开大学 | Ferulic acid and ferulic acid derivative anti-phytoviral agents |
| CN102106346A (en) * | 2009-12-24 | 2011-06-29 | 中国科学院上海生命科学研究院 | Water hyacinth inhibitor, preparation method and method for inhibiting water hyacinth by inhibitor |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1988002602A1 (en) * | 1986-10-08 | 1988-04-21 | Dumbroff Erwin B | Control of senescence in fruits, vegetables and flowers |
| CN102090412A (en) * | 2009-12-11 | 2011-06-15 | 南开大学 | Ferulic acid and ferulic acid derivative anti-phytoviral agents |
| CN102106346A (en) * | 2009-12-24 | 2011-06-29 | 中国科学院上海生命科学研究院 | Water hyacinth inhibitor, preparation method and method for inhibiting water hyacinth by inhibitor |
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