CN103518752A - Application of daucosterol compounds as antiviral agent - Google Patents
Application of daucosterol compounds as antiviral agent Download PDFInfo
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- CN103518752A CN103518752A CN201310463181.7A CN201310463181A CN103518752A CN 103518752 A CN103518752 A CN 103518752A CN 201310463181 A CN201310463181 A CN 201310463181A CN 103518752 A CN103518752 A CN 103518752A
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- mosaic virus
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Abstract
The invention discloses new uses of daucosterol compounds represented by formula (I) as an antiviral agent. The formula (I) is shown in the specification. The compounds can be used for controlling the squash mosaic virus, the watermelon mosaic virus, the tobacco mosaic virus and the wheat yellow dwarf virus disease or the rice stripe virus disease.
Description
Technical field
The present invention relates to a kind of pesticide field, relate in particular to Antiphytoviral field, further relate to a kind of daucosterol compounds as the new purposes of antivirotic.
Background technology
The viroses of plant worldwide constitute a serious threat to Crop yield and quality, and the underproduction causing every year reaches 10-30%, approximately 20,000,000,000 dollars of economic losses.China Shi Yige large agricultural country, the approximately tens billion of units of economic loss that because of virus disease, grain-production, horticultural crop, economic crops etc. caused every year.Therefore, in the urgent need to developing antiviral drugs.The research that has abroad strengthened this respect from the beginning of the sixties, the alginic acid junket liquid eggs white (Mosanon) of Japan's registration in 1985 was once used to prevent and treat tomato virus disease for [common extraction albumen and mushroom bacterium juice extract cartinellin (Lentemin).Artificial synthetic antiviral drugs has virazole (Virazole, Germany, 1972), morpholine methyl tetrahydropyrimidine (1982; 1996), benzoyl polyamine class (France, 1991), triazine derivative (Germany, 1992) etc., but less in field application.Domestic virus drugs research starts from the beginning of the eighties, and so far, the product of developing and registering has high fat film, NS-83 to increase the several prods such as anti-agent, viral A, ZhiBingLing(SIC), is to take chemicals as main mixture preparation mostly.The present situation of current domestic viral pesticide is: (1) is because virus is to host's obligate parasitism (specialization is parasitic), it copies institute's energy requirement and material is provided by host cell, although mixture preparation is more, but, lack and directly act on viral single compound pesticide species, cause control efficiency poor.(2) all to take Moroxydine Hydrochloride etc. be main component to the medicine overwhelming majority of current domestic registration, and Moroxydine Hydrochloride is for a long time for the treatment of clinical disease viral disease, if can cause medicament residue in the long-term use such as cereal crops and fruit and vegetable, long-term edible band residual food product can cause the human body problem that develops immunity to drugs conventionally.(3) chemical virus formulation for a long time, use in a large number, can cause serious " pesticide hazards ".Therefore, high activity antiviral drugs from biogenic, particularly plant resource searching with development of new, not only there is very high theory value, and guarantee Food Production at control crop virosis, in green ecological agricultural sustainable development, there is important practical significance.
Daucosterol (Daucosterol), another name: sitogluside, Daucosterol.Molecular formula C
35h
60o
6.Molecular weight 576.85.As shown in the formula (I) compound, belong to a kind of daucosterol compounds, be present in Malvaceae plant, in the plants such as fig, also contain daucosterol (the chemical constitution study ，Xu Xi section of root of common fig, Hu Jiang etc., medicine service and research, 2005 Jun; 5 (2) 138-140 pages).Daucosterol structural formula is as follows:
Formula (I)
Prior art thinks that this compound has antitumor, anti-inflammatory and acaricidal activity, yet the antivirus action of relevant daucosterol yet there are no bibliographical information.
Summary of the invention
The object of this invention is to provide a kind of new purposes as antivirotic as shown in the formula the daucosterol compounds shown in (I):
Formula (I).
Especially, the daucosterol compounds shown in above-mentioned formula (I) is used for preventing and treating custard squash mosaic virus (cucumber mosaic virus), watermelon mosaic virus sick (watermelon mosaic virus), tobacco mosaic virus disease (tobacco mosaic virus), yellow stunt of wheat viral disease (barley yellow dwarf virus) and stripe disease (rice stipe virus).
Formula shown (I) compound can be mixed and made into the regular dosage form on the agricultural chemicals such as aqua, soluble liquid, missible oil, microemulsion, suspending agent, aqueous emulsion, wetting powder, water dispersible granules with acceptable insecticides adjuvant on agricultural chemicals, and its concrete preparation is prepared with reference to method conventional on agricultural chemicals.
The invention has the beneficial effects as follows:
(1) the invention provides the daucosterol compounds shown in above-mentioned formula (I) for the new purposes of antivirotic.(2) the daucosterol compounds shown in above-mentioned formula (I), in antiviral activity, is compared existing antivirotic, such as take the antivirotic that Moroxydine Hydrochloride etc. is main component, has better effect, and its preventive effect is at least 75%.
embodiment
Below in conjunction with specific embodiment, further describe the present invention.
Embodiment 1: the control efficiency of formula of the present invention (I) compound to custard squash mosaic virus and watermelon mosaic virus.
(1), the preparation of inoculation seedling: by custard squash and watermelon seed first with hoting water treatment of seeds 30 minutes, then, vernalization in culture dish, treat that bud grows to 1cm left and right, selecting consistent bud is sowed in flowerpot, be put in the greenhouse of fly net and cultivate, while growing to two cotyledon one lobus cardiacuses, inoculate and spray medicine and process;
(2), processing method: test minute 4 processing, each processes 6 strains, be provided with morbidity contrast (only inoculation, do not spray medicine), blank (is not inoculated, do not spray medicine), spray medicine processes that (first inoculation, sprays formula of the present invention (I) compound and control drug (20% Moroxydine Hydrochloride copper acetate wetting powder) after 24h, the 7th day Sampling Survey after spray medicine;
(3), inoculum density: inoculum density is 8%, and method is to get the obvious fresh blade of 2.5g morbidity on the malicious source plant of breeding, adds a small amount of 1%Na in mortar
2hPO
4; (pH=7.0) buffer solution and a small amount of 400 order diamond dust, fully grind to form homogenate, then add appropriate amount of buffer solution, mixes, and then uses four layers of filtered through gauze, and filtrate is settled to 50ml with buffer solution;
(4), inoculation method: adopt juice frictional inoculation, dip appropriate inoculation liquid with finger, rub gently 3 times on each inoculation blade to same direction, the about 2-3cm of area, finally rinses inoculation blade surface with clear water;
(5), spraying concentration and method: by 100 times of formula of the present invention (I) diluted chemical compounds, spray medicine after inoculating 24 hours.With miniaturised nebuliser, spray and supplying on examination custard squash and water melon leaf, until leaf surface is evenly covered with liquid;
(6), state of an illness investigation: process the incidence of plant at 7 days " Invest, Then Investigate " custard squashs of spray medicine and watermelon;
(7), result of the test: at custard squash and watermelon their early stage, investigation statistics morbidity and growth growth pattern, from the result of table 1 and table 2, show, it is 83.3% and 78.6% to the average relative curative effect of custard squash and watermelon mosaic virus disease that formula of the present invention (I) compounds for treating is processed.
The control efficiency of table 1 formula of the present invention (I) compound to custard squash mosaic virus
The control efficiency of table 2 formula of the present invention (I) compound to watermelon mosaic virus disease
Embodiment 2: the control in field effect of formula of the present invention (I) compound to tobacco mosaic virus and wheat yellow dwarf virus
1, experiment condition: crop choice tobacco and wheat; Target is tobacco mosaic virus and wheat yellow dwarf virus;
2, experimental scheme and arrangement: test medicine is formula of the present invention (I) compound, set up 20% Moroxydine Hydrochloride copper acetate wetting powder contrast medicament and clear water contrast.Totally 3 processing, repeat 3 times, totally 9 communities.Arrange by random district group experimental plot;
3. application method: transplant and carry out spray pesticide in latter 20 days, sprayed for the second time medicine after 7 days;
4. experimental result:
The control efficiency of table 3 formula of the present invention (I) compound to tobacco mosaic virus disease
The control efficiency of table 4 formula of the present invention (I) compound to yellow stunt of wheat viral disease
Embodiment 3: formula of the present invention (I) compound is to stripe disease field controling test
1, experiment condition: crop is paddy rice, kind is Shengdao301, rice transplanting on June 5; Subjects is: target is stripe disease (rice stipe virus).Experimental field is rice cultivation year after year, and irrigation condition is better.
2. experimental scheme and arrangement: test medicine is 100 times of formula of the present invention (I) compounds, sets up 100 times of contrast medicament 20% Moroxydine Hydrochloride copper acetate wetting powders and clear water contrast.Totally 3 processing, repeat 4 times, Gong12Ge community, community area 20m
2, minizone is provided with protection row.Arrange by random district group experimental plot.
3. application method: transplant and within latter 30 days, carry out spray pesticide, after medication 1 time after 7 days, dispenser is 3 times altogether.
4. experimental result:
The control efficiency of table 5 formula of the present invention (I) compound to stripe disease
As can be seen from the above embodiments, daucosterol compounds shown in above-mentioned formula (I) has good preventive effect to custard squash mosaic virus, watermelon mosaic virus disease, tobacco mosaic virus disease, yellow stunt of wheat viral disease and stripe disease, is obviously better than contrasting the preventive effect of medicament 20% Moroxydine Hydrochloride copper acetate wetting powder to above-mentioned disease.Formula (I) compound is medicine based on herbs, and safety and environmental protection has broad application prospects in agricultural production.
A kind of daucosterol compounds of the present invention is described by concrete example as the application of antivirotic, those skilled in the art can use for reference content of the present invention, the links such as appropriate change raw material, process conditions realize corresponding other object, its relevant change does not all depart from content of the present invention, within all similar replacements and change will become apparent to those skilled in the art that and be all deemed to be included in scope of the present invention.
Claims (4)
- One kind as shown in the formula the daucosterol compounds shown in (I) as the new purposes of antivirotic:
Formula (I). - 2. new purposes according to claim 1, is used for preventing and treating crop disease viral disease by the daucosterol compounds shown in formula (I).
- 3. crop disease viral disease according to claim 2 is custard squash mosaic virus (cucumber mosaic virus), watermelon mosaic virus sick (watermelon mosaic virus), tobacco mosaic virus disease (tobacco mosaic virus), yellow stunt of wheat viral disease (barley yellow dwarf virus) or stripe disease (rice stipe virus).
- 4. new purposes according to claim 1, on described formula (I) compound and agricultural chemicals, acceptable insecticides adjuvant is mixed and made into aqua, soluble liquid, missible oil, microemulsion, suspending agent, aqueous emulsion, wetting powder or water dispersible granules.
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| CN103518752B CN103518752B (en) | 2014-12-10 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104738102A (en) * | 2015-03-26 | 2015-07-01 | 杨凌农科大无公害农药研究服务中心 | Application of actinidia chinensis root-bark extract for preparing virucide for plants |
Citations (4)
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|---|---|---|---|---|
| EP0509656A1 (en) * | 1991-03-28 | 1992-10-21 | Rooperol (Na) Nv | Compositions of phytosterols and phytosterolins as immunmodulators |
| EP0587374A2 (en) * | 1992-09-02 | 1994-03-16 | Virostat (Na) Nv | Sulfated sterol glycosides and their use against viral infections. |
| CN102273470A (en) * | 2011-05-27 | 2011-12-14 | 北京农学院 | Daucosterol plant miticide and purpose thereof |
| CN103083368A (en) * | 2013-02-05 | 2013-05-08 | 中国林业科学研究院林产化学工业研究所 | Preparation method of ginkgo leaf lipoid components having antibacterial activities |
-
2013
- 2013-10-08 CN CN201310463181.7A patent/CN103518752B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0509656A1 (en) * | 1991-03-28 | 1992-10-21 | Rooperol (Na) Nv | Compositions of phytosterols and phytosterolins as immunmodulators |
| EP0587374A2 (en) * | 1992-09-02 | 1994-03-16 | Virostat (Na) Nv | Sulfated sterol glycosides and their use against viral infections. |
| CN102273470A (en) * | 2011-05-27 | 2011-12-14 | 北京农学院 | Daucosterol plant miticide and purpose thereof |
| CN103083368A (en) * | 2013-02-05 | 2013-05-08 | 中国林业科学研究院林产化学工业研究所 | Preparation method of ginkgo leaf lipoid components having antibacterial activities |
Non-Patent Citations (4)
| Title |
|---|
| GERALDO CÉLIO BRANDÃO等: "Antimicrobial, antiviral and cytotoxic activity of extracts and constituents from Polygonum spectabile Mart.", 《PHYTOMEDICINE》 * |
| PING-CHUNG KUO等: "Chemical Constituents from Lobelia chinensis and Their Anti-virus and Anti-inflammatory Bioactivities", 《ARCH PHARM RES》 * |
| 徐希科等: "无花果根化学成分研究", 《药学服务与研究》 * |
| 梁红艳等: "木瓜抗烟草花叶病毒活性及其有效成分分离", 《木瓜抗烟草花叶病毒活性及其有效成分分离》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104738102A (en) * | 2015-03-26 | 2015-07-01 | 杨凌农科大无公害农药研究服务中心 | Application of actinidia chinensis root-bark extract for preparing virucide for plants |
| CN104738102B (en) * | 2015-03-26 | 2017-12-15 | 杨凌农科大无公害农药研究服务中心 | Chinese gooseberry root bark extract is used for the application for preparing anti-plant virus agent |
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| CN103518752B (en) | 2014-12-10 |
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