CN110972826A - Seedling growing method of epimedium - Google Patents
Seedling growing method of epimedium Download PDFInfo
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- CN110972826A CN110972826A CN201911190805.6A CN201911190805A CN110972826A CN 110972826 A CN110972826 A CN 110972826A CN 201911190805 A CN201911190805 A CN 201911190805A CN 110972826 A CN110972826 A CN 110972826A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
The invention discloses a seedling raising method of epimedium herb, and belongs to the field of crop seedling raising. The method comprises the following steps: s1, soaking seeds and drying; s2, seed temperature changing lamination treatment; s3, seed soaking agent treatment: soaking seeds of herba Epimedii in a mixture of sodium carboxymethylcellulose, ascorbic acid and gibberellin for 1.5-2 h; laminating the soaked seeds at constant temperature of 10 deg.C for 15-30 days; and S4, sowing seeds. The method for culturing the epimedium seedlings has the advantages that the germination rate is 89.5-92.8%, the incidence rate of seedling diseases is greatly reduced, and particularly, no polluting reagent is used in the method, so that the method has the advantages of safety and environmental protection.
Description
Technical Field
The invention belongs to the field of crop seedling culture, and particularly relates to a seedling culture method of epimedium.
Background
Herba Epimedii is perennial herb with height of 20-60cm, thick and short rhizome, dark brown color, two-time and three-time double-emergence compound leaf basal growth and stem growth, long stem, small leaf paper or thick paper, leaf margin with thorn teeth, white or light yellow flower, flowering period of 5-6 months, and fruit period of 6-8 months. The epimedium herb is used for medicine. It can be used for treating sexual impotence, premature ejaculation, soreness of waist, skelalgia, numbness of limbs, hemiplegia, neurasthenia, amnesia, tinnitus, and blurred vision. The epimedium fruits belong to capsules, and the seeds are scattered immediately when the capsules crack while being mature, so that the epimedium cannot be collected intensively. The mass of each thousand mature seeds of epimedium is only 2.3-2.4g, the seeds are fine and difficult to collect after falling with wind. In addition, the seeds obtained by collecting the fruits when the epimedium capsule is not cracked are immature seeds which cannot germinate.
The existing literature search shows that Chinese patent publication No. CN 104770099A, published as 2018, 10, month and 12, discloses a seedling raising method of epimedium wushanense, which comprises the following steps: step (1): collecting epimedium wushanense seeds and carrying out pretreatment; step (2): carrying out temperature-changing stratification treatment on epimedium wushanense seeds; and (3): soaking seeds of epimedium wushanense subjected to temperature-variable stratification by using seed soaking liquid containing fluazinone. The method adopts gibberellin and fluazinone aqueous solution to soak seeds, and combines low-temperature stratification technology, so that the germination rate, the germination potential and the germination index of epimedium wushanense seeds are obviously improved, and the average germination time is obviously reduced. However, the method uses the fluazinone, so that the property is unstable, the pollution to the water body is great, and the long-term adverse effect on the water body environment is possibly generated. .
Disclosure of Invention
Aiming at solving the problem of large pollution of the existing seedling raising method, the epimedium seed seedling raising method which has high germination rate and no pollution is provided.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for raising seedlings of epimedium comprises the following steps:
s1, seed soaking and drying: soaking herba Epimedii seed in 18-22 deg.C sterile water for 12-18 hr, taking out, draining, and oven drying at 30-35 deg.C until water content is 12-15%;
s2, seed temperature change lamination treatment: mixing dried and sterilized turfy soil and vermiculite according to the mass ratio of 1:5-9 to prepare a mixed matrix, adding sterile water accounting for 18-20% of the mass of the mixed matrix, and fully and uniformly mixing to obtain a water-containing matrix; mixing and seed-dressing the seeds for standby use and a water-containing matrix according to a mass ratio of 1:5, then placing for 12h at 8 ℃, placing for 12h at 20 ℃, alternately performing temperature-changing lamination for 90-120 days, smashing seeds once every 15 days in the temperature-changing lamination process, and keeping the water content of the matrix at 18-20%;
s3, seed soaking agent treatment: soaking seeds of herba Epimedii in a mixture of sodium carboxymethylcellulose, ascorbic acid and gibberellin for 1.5-2 h; laminating the soaked seeds at constant temperature of 10 deg.C for 15-30 days;
s4, sowing: watering the substrate to enable the water content of the substrate to be 20-30%, sowing the seeds treated by the seed soaking agent in the substrate, covering a mulching film, and transplanting the seeds at a proper time when the seeds germinate and grow to 8-10 cm.
Preferably, the mass concentration of the sodium carboxymethyl cellulose is 200-300 g/L; the mass concentration of the ascorbic acid is 30-50 mg/L; the mass concentration of the gibberellin is 200-300 mg/L.
Preferably, the matrix comprises, by mass: 5% of bamboo shoot processing waste, 25% of perlite, 10% of bone meal, 12% of decomposed pig manure, 10% of turfy soil and 38% of vermiculite.
Preferably, the sowing amount of the epimedium seeds is 1.0-1.2g/m2。
Compared with the prior art, the invention has the beneficial technical effects that:
1) the invention adopts the technical means of combining low-temperature water seed soaking, variable-temperature lamination and constant-temperature lamination. Wherein the sodium carboxymethylcellulose is a cellulose derivative, has the functions of water retention, stability and the like, and can be used as an emulsion stabilizer, a film-forming agent and a carrier; the ascorbic acid is a water-soluble vitamin, and can improve the activity of related enzymes, promote the generation of interferon and inhibit the proliferation of viruses; gibberellin is a regulator widely used in production for promoting dormancy breaking of seeds, and can break the dormancy period of epimedium seeds, but the action effect is not obvious. The invention combines the sodium carboxymethylcellulose, the ascorbic acid and the gibberellin and then soaks the epimedium seeds, and the interaction of the three is generated, thereby obviously enhancing the effect of relieving the dormant period of the epimedium seeds. By adopting the method, the germination rate of the epimedium seeds is obviously improved, and the average germination time is obviously reduced.
2) The method of the invention is adopted to carry out epimedium seedling raising, the germination rate is as high as 89.5-92.8%, the incidence rate of seedling diseases is greatly reduced, and particularly, the method of the invention does not use polluting reagents and has the advantages of safety and environmental protection.
Detailed Description
The technical solution of the present invention will be further specifically described below by way of specific examples. It is to be understood that the embodiments of the present invention are not limited to the following embodiments, and that any changes and/or modifications may be made to the present invention within the scope of the present invention.
Example 1
A method for raising seedlings of epimedium comprises the following steps:
s1, seed soaking and drying: soaking herba Epimedii seed in 20 deg.C sterile water at constant temperature for 18 hr, taking out, draining, and oven drying at 30 deg.C to water content of 12%;
s2, seed temperature change lamination treatment: mixing dried and sterilized turfy soil and vermiculite according to a mass ratio of 1:5 to prepare a mixed matrix, adding sterile water accounting for 18% of the mass of the mixed matrix, and fully and uniformly mixing to obtain a water-containing matrix; mixing and seed-dressing the seeds for later use and a water-containing matrix according to the mass ratio of 1:5, then placing for 12h at 8 ℃, placing for 12h at 20 ℃, alternately carrying out temperature-changing lamination for 90 days, smashing seeds once every 15 days in the temperature-changing lamination process, and keeping the water content of the matrix at 18%;
s3, seed soaking agent treatment: soaking seeds of herba Epimedii in a combination solution of sodium carboxymethylcellulose, ascorbic acid and gibberellin for 1.5 h; laminating the soaked seeds at a constant temperature of 10 ℃ for 30 days, wherein the mass concentration of the sodium carboxymethyl cellulose is 200 g/L; the mass concentration of the ascorbic acid is 30 mg/L; the mass concentration of the gibberellin is 200 mg/L;
s4, sowing: watering the matrix to make the water content of the matrix be 25%, and sowing the seeds treated by the seed soaking agent according to the sowing quantity of 1.0g/m2Sowing in the substrate, covering with mulching film, and transplanting when the seeds germinate to 10cm, wherein the substrate comprises massAnd (3) ratio measurement: 5% of bamboo shoot processing waste, 25% of perlite, 10% of bone meal, 12% of decomposed pig manure, 10% of turfy soil and 38% of vermiculite.
Example 2
A method for raising seedlings of epimedium comprises the following steps:
s1, seed soaking and drying: soaking herba Epimedii seed in 25 deg.C sterile water for 12 hr, taking out, draining, and oven drying at 35 deg.C to water content of 15%;
s2, seed temperature change lamination treatment: mixing dried and sterilized turfy soil and vermiculite according to a mass ratio of 1:9 to prepare a mixed matrix, adding sterile water accounting for 20% of the mass of the mixed matrix, and fully and uniformly mixing to obtain a water-containing matrix; mixing and seed-dressing the seeds for later use and a water-containing matrix according to the mass ratio of 1:5, then placing for 12h at 8 ℃, placing for 12h at 20 ℃, alternately performing temperature-changing lamination for 120 days, smashing seeds once every 15 days in the temperature-changing lamination process, and keeping the water content of the matrix at 20%;
s3, seed soaking agent treatment: soaking seeds of herba Epimedii in a combination solution of sodium carboxymethylcellulose, ascorbic acid and gibberellin for 1.5 h; laminating the soaked seeds at a constant temperature of 10 ℃ for 22 days, wherein the mass concentration of the sodium carboxymethyl cellulose is 300 g/L; the mass concentration of the ascorbic acid is 50 mg/L; the mass concentration of the gibberellin is 300 mg/L;
s4, sowing: watering the matrix to make the water content of the matrix be 30%, and sowing the seeds treated by the seed soaking agent according to the sowing quantity of 1.0g/m2Sowing the seeds in the substrate, covering with a mulching film, and transplanting the seeds at a proper time when the seeds germinate and grow to 10cm, wherein the substrate comprises the following components in percentage by mass: 5% of bamboo shoot processing waste, 25% of perlite, 10% of bone meal, 12% of decomposed pig manure, 10% of turfy soil and 38% of vermiculite.
Example 3
A method for raising seedlings of epimedium comprises the following steps:
s1, seed soaking and drying: soaking herba Epimedii seed in 22 deg.C sterile water, soaking at constant temperature for 15 hr, taking out, draining, and oven drying at 32 deg.C until water content is 12%;
s2, seed temperature change lamination treatment: mixing dried and sterilized turfy soil and vermiculite according to a mass ratio of 1:7 to prepare a mixed matrix, adding sterile water accounting for 20% of the mass of the mixed matrix, and fully and uniformly mixing to obtain a water-containing matrix; mixing and seed-dressing the seeds for later use and a water-containing matrix according to the mass ratio of 1:5, then placing for 12h at 8 ℃, placing for 12h at 20 ℃, alternately carrying out temperature-changing lamination for 90 days, smashing seeds once every 15 days in the temperature-changing lamination process, and keeping the water content of the matrix at 20%;
s3, seed soaking agent treatment: soaking seeds of herba Epimedii in a combination solution of sodium carboxymethylcellulose, ascorbic acid and gibberellin for 1.5 h; laminating the soaked seeds at a constant temperature of 10 ℃ for 15 days, wherein the mass concentration of the sodium carboxymethyl cellulose is 240 g/L; the mass concentration of the ascorbic acid is 40 mg/L; the mass concentration of the gibberellin is 280 mg/L;
s4, sowing: watering the matrix to make the water content of the matrix be 20%, and sowing the seeds treated by the seed soaking agent according to the sowing quantity of 1.2g/m2Sowing the seeds in the substrate, covering with a mulching film, and transplanting the seeds at a proper time when the seeds germinate and grow to 10cm, wherein the substrate comprises the following components in percentage by mass: 5% of bamboo shoot processing waste, 25% of perlite, 10% of bone meal, 12% of decomposed pig manure, 10% of turfy soil and 38% of vermiculite.
The invention researches the influence of the seed soaking agent on the germination rate of epimedium seeds, observes the influence of the component change of the seed soaking agent on the germination rate (90 days) under the condition of the same other influences and steps, and records the sodium carboxymethylcellulose as A for convenient recording; ascorbic acid is marked as B; gibberellins are designated as C, and the results are shown in Table 1 below.
TABLE 1 Effect of different components of seed-soaking Agents on germination percentage
As can be seen from the above table 1, when the seed is soaked by the combined solution of sodium carboxymethylcellulose, ascorbic acid and gibberellin, the germination rate of the epimedium seeds is high and reaches about 90%. Meanwhile, the comparison shows that the mixing of the three components, namely the sodium methylcellulose, the ascorbic acid and the gibberellin has an obvious synergistic effect compared with the mixing of any two components, so that certain interaction can be generated when the three components are mixed, and the dormancy period of the epimedium seeds is effectively relieved.
The present inventors also studied the incidence of diseases in the epimedium obtained by the method of the present invention during growth, and the results are shown in table 2.
TABLE 2 incidence of disease in Epimedium during growth
As can be seen from Table 2, how large amount of epimedium obtained by the method has low disease incidence rate during growth and obvious disease resistance effect.
The present invention may be embodied in other specific forms without departing from its spirit or essential characteristics. The above-described embodiments of the invention are to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Claims (4)
1. A method for raising seedlings of epimedium herb is characterized by comprising the following steps: the method comprises the following steps:
s1, seed soaking and drying: soaking herba Epimedii seed in 20-25 deg.C sterile water for 12-18 hr, taking out, draining, and oven drying at 30-35 deg.C until water content is 12-15%;
s2, seed temperature change lamination treatment: mixing dried and sterilized turfy soil and vermiculite according to the mass ratio of 1:5-9 to prepare a mixed matrix, adding sterile water accounting for 18-20% of the mass of the mixed matrix, and fully and uniformly mixing to obtain a water-containing matrix; mixing and seed-dressing the seeds for standby use and a water-containing matrix according to a mass ratio of 1:5, then placing for 12h at 8 ℃, placing for 12h at 20 ℃, alternately performing temperature-changing lamination for 90-120 days, smashing seeds once every 15 days in the temperature-changing lamination process, and keeping the water content of the matrix at 18-20%;
s3, seed soaking agent treatment: soaking seeds of herba Epimedii in a mixture of sodium carboxymethylcellulose, ascorbic acid and gibberellin for 1.5-2 h; laminating the soaked seeds at constant temperature of 10 deg.C for 15-30 days;
s4, sowing: watering the substrate to enable the water content of the substrate to be 20-30%, sowing the seeds treated by the seed soaking agent in the substrate, covering a mulching film, and transplanting the seeds at a proper time when the seeds germinate and grow to 8-10 cm.
2. The method for raising seedlings of epimedium herb according to claim 1, which is characterized in that: the mass concentration of the sodium carboxymethylcellulose is 200-300 g/L; the mass concentration of the ascorbic acid is 30-50 mg/L; the mass concentration of the gibberellin is 200-300 mg/L.
3. A method for raising seedlings of epimedium herb according to claim 1, wherein the substrate comprises the following components in percentage by mass: 5% of bamboo shoot processing waste, 25% of perlite, 10% of bone meal, 12% of decomposed pig manure, 10% of turfy soil and 38% of vermiculite.
4. A method for growing seedlings of herba Epimedii according to claim 1, wherein the seeding amount of the herba Epimedii seed is 1.0-1.2g/m2。
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111657068A (en) * | 2020-06-17 | 2020-09-15 | 劲牌有限公司 | Method for breeding epimedium seedlings |
| CN114946318A (en) * | 2022-06-13 | 2022-08-30 | 广东省农业科学院农业生物基因研究中心 | Method for promoting germination of seeds of Epimedium plants |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111657068A (en) * | 2020-06-17 | 2020-09-15 | 劲牌有限公司 | Method for breeding epimedium seedlings |
| CN114946318A (en) * | 2022-06-13 | 2022-08-30 | 广东省农业科学院农业生物基因研究中心 | Method for promoting germination of seeds of Epimedium plants |
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