CN103518752B - Application of daucosterol compounds as antiviral agent - Google Patents
Application of daucosterol compounds as antiviral agent Download PDFInfo
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- CN103518752B CN103518752B CN201310463181.7A CN201310463181A CN103518752B CN 103518752 B CN103518752 B CN 103518752B CN 201310463181 A CN201310463181 A CN 201310463181A CN 103518752 B CN103518752 B CN 103518752B
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Abstract
The invention discloses new uses of daucosterol compounds represented by formula (I) as an antiviral agent. The formula (I) is shown in the specification. The compounds can be used for controlling the squash mosaic virus, the watermelon mosaic virus, the tobacco mosaic virus and the wheat yellow dwarf virus disease or the rice stripe virus disease.
Description
Technical field
The present invention relates to a kind of pesticide field, relate in particular to Antiphytoviral field, further relate to the new purposes of a kind of daucosterol compounds as antivirotic.
Background technology
The viroses of plant worldwide constitute a serious threat to Crop yield and quality, and the underproduction causing every year reaches 10-30%, approximately 20,000,000,000 dollars of economic losses.China is a large agricultural country, the approximately tens billion of units of economic loss that because of virus disease, grain-production, horticultural crop, economic crops etc. caused every year.Therefore, in the urgent need to developing antiviral drugs.The research that has abroad strengthened this respect from the beginning of the sixties, the alginic acid junket liquid eggs white (Mosanon) of Japan's registration in 1985 was once used to prevent and treat tomato virus disease for [common extraction albumen and mushroom bacterium juice extract cartinellin (Lentemin).Artificial synthetic antiviral drugs has virazole (Virazole, Germany, 1972), morpholine methyl tetrahydropyrimidine (1982; 1996), benzoyl polyamine class (France, 1991), triazine derivative (Germany, 1992) etc., but less in field application.Domestic virus drugs research starts from the beginning of the eighties, and so far, the product of developing and registering has high fat film, NS-83 to increase the several prods such as anti-agent, viral A, ZhiBingLing(SIC), is taking chemicals as main mixture preparation mostly.The present situation of current domestic viral pesticide is: (1) is due to the obligate parasitism (specialization parasitic) of virus to host, it copies institute's energy requirement and material is provided by host cell, although mixture preparation is more, but, lack and directly act on viral single compound pesticide species, cause control efficiency poor.(2) the medicine overwhelming majority of current domestic registration is all taking Moroxydine Hydrochloride etc. as main component, and Moroxydine Hydrochloride is for a long time for the treatment of clinical disease viral disease, if can cause medicament residue in the long-term use such as cereal crops and fruit and vegetable, long-term edible band residual food product can cause the human body problem that develops immunity to drugs conventionally.(3) chemical virus formulation for a long time, use in a large number, can cause serious " pesticide hazards ".Therefore, high activity antiviral drugs from biogenic, particularly plant resource searching with development of new, not only there is very high theory value, and guarantee Food Production at control crop virosis, in green ecological agricultural sustainable development, there is important practical significance.
Daucosterol (Daucosterol), another name: sitogluside, Daucosterol.Molecular formula C
35h
60o
6.Molecular weight 576.85.Belong to a kind of daucosterol compounds as shown in the formula (I) compound, be present in Malvaceae plant, in the plants such as fig, also contain daucosterol (chemical constitution study of root of common fig, Xu Xike, Hu Jiang etc., medicine service and research, 2005 Jun; 5 (2) 138-140 pages).Daucosterol structural formula is as follows:
Formula (I)
Prior art thinks that this compound has antitumor, anti-inflammatory and acaricidal activity, but yet there are no bibliographical information about the antivirus action of daucosterol.
Summary of the invention
The object of this invention is to provide a kind of new purposes as antivirotic as shown in the formula the daucosterol compounds shown in (I):
Formula (I).
Especially, the daucosterol compounds shown in above-mentioned formula (I) is used for preventing and treating custard squash mosaic virus (zucchini mosaic virus), watermelon mosaic virus disease (watermelon mosaic virus), tobacco mosaic virus disease (tobacco mosaic virus), yellow stunt of wheat viral disease (Wheat yellow dwarf virus) and stripe disease (rice stipe virus).
Shown in formula (I) compound can be mixed and made into the regular dosage form on the agricultural chemicals such as aqua, soluble liquid, missible oil, microemulsion, suspending agent, aqueous emulsion, wetting powder, water dispersible granules with acceptable insecticides adjuvant on agricultural chemicals, its concrete preparation is prepared with reference to method conventional on agricultural chemicals.
The invention has the beneficial effects as follows:
(1) the invention provides the new purposes of the daucosterol compounds shown in above-mentioned formula (I) for antivirotic.
(2) the daucosterol compounds shown in above-mentioned formula (I), in antiviral activity, is compared existing antivirotic, and for example the antivirotic taking Moroxydine Hydrochloride etc. as main component, has better effect, and its preventive effect is at least 75%.
Embodiment
Further describe the present invention below in conjunction with specific embodiment.
Embodiment 1: the control efficiency of formula of the present invention (I) compound to custard squash mosaic virus and watermelon mosaic virus.
(1), the preparation of inoculation seedling: by custard squash and watermelon seed first with hoting water treatment of seeds 30 minutes, then, vernalization in culture dish, treat that bud grows to 1cm left and right, selecting consistent bud is sowed in flowerpot, be put in the greenhouse of fly net and cultivate, while growing to two cotyledon one lobus cardiacuses, inoculate and spray medicine processing.
(2), processing method: test point 4 processing, each processing 6 strains, be provided with morbidity contrast (only inoculation, do not spray medicine), blank (is not inoculated, do not spray medicine), (first inoculation, sprays formula of the present invention (I) compound and control drug (20% Moroxydine Hydrochloride copper acetate wetting powder) after 24h, the 7th day Sampling Survey after spray medicine in spray medicine processing;
(3), inoculum density: inoculum density is 8%, and method is to get the obvious fresh blade of 2.5g morbidity on the malicious source plant of breeding, adds a small amount of 1%Na in mortar
2hPO
4; (pH=7.0) buffer solution and a small amount of 400 order diamond dust, fully grind to form homogenate, then add appropriate amount of buffer solution, mixes, and then uses four layers of filtered through gauze, and filtrate is settled to 50ml with buffer solution;
(4), inoculation method: adopt juice frictional inoculation, dip appropriate inoculation liquid with pointing, rub gently 3 times on each inoculation blade to same direction, the about 2-3cm of area, finally rinses inoculation blade surface with clear water;
(5), spraying concentration and method: by 100 times of formula of the present invention (I) diluted chemical compounds, inoculate and spray medicine after 24 hours.Spray and supplying on examination custard squash and water melon leaf, until leaf surface is evenly covered with liquid with miniaturised nebuliser;
(6), state of an illness investigation: process the incidence of plant at 7 days " Invest, Then Investigate " custard squashs of spray medicine and watermelon.
(7), result of the test: at custard squash and watermelon their early stage, investigation statistics morbidity and growth growth pattern, show from the result of table 1 and table 2, it is 83.3% and 78.6% to the average relative curative effect of custard squash and watermelon mosaic virus disease that formula of the present invention (I) compounds for treating is processed.
The control efficiency of table 1 formula of the present invention (I) compound to custard squash mosaic virus
The control efficiency of table 2 formula of the present invention (I) compound to watermelon mosaic virus disease
Embodiment 2: the control in field effect of formula of the present invention (I) compound to tobacco mosaic virus and wheat yellow dwarf virus
1, experiment condition: crop choice tobacco and wheat; Target is tobacco mosaic virus and wheat yellow dwarf virus.
2, experimental scheme and arrangement: test medicine is formula of the present invention (I) compound, sets up 20% Moroxydine Hydrochloride copper acetate wetting powder contrast medicament and clear water contrast.Totally 3 processing, repeat 3 times, 9 communities totally.Arrange by random district group experimental plot.
3. application method: transplant and carry out spray pesticide in latter 20 days, sprayed for the second time medicine after 7 days.
4. experimental result:
The control efficiency of table 3 formula of the present invention (I) compound to tobacco mosaic virus disease
The control efficiency of table 4 formula of the present invention (I) compound to yellow stunt of wheat viral disease
Embodiment 3: formula of the present invention (I) compound is to stripe disease field controling test
1, experiment condition: crop is paddy rice, kind is Shengdao301, rice transplanting on June 5; Subjects is: target is stripe disease (rice stipe virus).Experimental field is rice cultivation year after year, and irrigation condition is better.
2. experimental scheme and arrangement: test medicine is 100 times of formula of the present invention (I) compounds, sets up 100 times of contrast medicament 20% Moroxydine Hydrochloride copper acetate wetting powders and clear water contrast.Totally 3 processing, repeat 4 times, Gong12Ge community, community area 20m
2, minizone is provided with protection row.Arrange by random district group experimental plot.
3. application method: transplant and within latter 30 days, carry out spray pesticide, after medication 1 time after 7 days, altogether dispenser 3 times.
4. experimental result:
The control efficiency of table 5 formula of the present invention (I) compound to stripe disease
As can be seen from the above embodiments, daucosterol compounds shown in above-mentioned formula (I) has good preventive effect to custard squash mosaic virus, watermelon mosaic virus disease, tobacco mosaic virus disease, yellow stunt of wheat viral disease and stripe disease, is obviously better than contrasting the preventive effect of medicament 20% Moroxydine Hydrochloride copper acetate wetting powder to above-mentioned disease.Formula (I) compound is medicine based on herbs, and safety and environmental protection has broad application prospects in agricultural production.
A kind of daucosterol compounds of the present invention is described by concrete example as the application of antivirotic, those skilled in the art can use for reference content of the present invention, the links such as appropriate change raw material, process conditions realize corresponding other object, its relevant change does not all depart from content of the present invention, within all similar replacements and change will become apparent to those skilled in the art that and be all deemed to be included in scope of the present invention.
Claims (3)
- One kind as shown in the formula the daucosterol compounds shown in (I) the new purposes for the preparation of anti-crop disease toxic agent:Formula (I).
- 2. new purposes according to claim 1, daucosterol compounds shown in formula (I) is used for preventing and treating crop disease viral disease, and described crop disease viral disease is custard squash mosaic virus, watermelon mosaic virus disease, tobacco mosaic virus disease, yellow stunt of wheat viral disease or stripe disease.
- 3. new purposes according to claim 1, on described formula (I) compound and agricultural chemicals, acceptable insecticides adjuvant is mixed and made into aqua, soluble liquid, missible oil, microemulsion, suspending agent, aqueous emulsion, wetting powder or water dispersible granules.
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| CN104738102B (en) * | 2015-03-26 | 2017-12-15 | 杨凌农科大无公害农药研究服务中心 | Chinese gooseberry root bark extract is used for the application for preparing anti-plant virus agent |
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| CN102273470A (en) * | 2011-05-27 | 2011-12-14 | 北京农学院 | Daucosterol plant miticide and purpose thereof |
| CN103083368A (en) * | 2013-02-05 | 2013-05-08 | 中国林业科学研究院林产化学工业研究所 | Preparation method of ginkgo leaf lipoid components having antibacterial activities |
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2013
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|---|---|---|---|---|
| EP0509656A1 (en) * | 1991-03-28 | 1992-10-21 | Rooperol (Na) Nv | Compositions of phytosterols and phytosterolins as immunmodulators |
| EP0587374A2 (en) * | 1992-09-02 | 1994-03-16 | Virostat (Na) Nv | Sulfated sterol glycosides and their use against viral infections. |
| CN102273470A (en) * | 2011-05-27 | 2011-12-14 | 北京农学院 | Daucosterol plant miticide and purpose thereof |
| CN103083368A (en) * | 2013-02-05 | 2013-05-08 | 中国林业科学研究院林产化学工业研究所 | Preparation method of ginkgo leaf lipoid components having antibacterial activities |
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| atilde * |
| Chemical Constituents from Lobelia chinensis and Their Anti-virus and Anti-inflammatory Bioactivities;Ping-Chung Kuo等;《Arch Pharm Res》;20111231;第34卷(第5期);715-722 * |
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