CN104721246B - A kind of natural plant extracts preparation for being used to prevent transient cerebral ischemia - Google Patents
A kind of natural plant extracts preparation for being used to prevent transient cerebral ischemia Download PDFInfo
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Abstract
A kind of natural plant extracts preparation for being used to prevent transient cerebral ischemia, belongs to health product technology field.By myrica ruba marc extract, plumage awns chrysanthemum general flavone, yeast selenium according to 50 80g of weight ratio:20‑50 g:300 800 μ g are uniformly mixed.A kind of above-mentioned natural plant extracts preparation for being used to prevent transient cerebral ischemia, its functional component content is high, easy to use and carry, oral absorption is rapid, bioavilability is high, prevention effect is obvious, preparation process is simple, easy to operate, and working condition is easy to control, the degree of automation and production efficiency are high, it is green safe, can industrialized production, opened up a new way for prevention transient cerebral ischemia and using natural resources.
Description
Technical field
The invention belongs to health product technology field, is specially a kind of natural plant extracts for being used to prevent transient cerebral ischemia
Preparation.
Background technology
As population is gradually aging, the incidence of ischemic cerebrovascular disease raises year by year, becomes threaten the mankind at present
One of most important disease of life, the serious prestige caused by its high lethality and disability rate give society and family
The side of body, becomes the hot subject of modern medicine study at present.Brain is the human body organ most sensitive to anoxic, brain tissue ischemia meeting
Cause the infringement of local brain tissue and its function, how much related its extent of damage and Ischemia Time length and remaining blood flow is, short
Phase imperfection ischemic only causes invertibity to be damaged, and prolonged ischemic or severe ischemic completely can cause cerebral infarction.Brain is most
Obvious histological change is encephaledema and brain cell necrosis.The generation of encephaledema is that membrane lipid peroxidatio destroys the structure of film
With the result of dysfunction of sodium pump.The research of the damage mechanisms of ishemic stroke is just progressively goed deep into, except excitatory toxicity, calcium overload
Outside, the damage of Apoptosis, inflammation, free radical, nitric oxide and mitochondria is further included, meanwhile, body also has the guarantor of inherence
Protection mechanism.The principle for clinically handling cerebral ischemic injury is to recover blood reperfusion as early as possible, retrieves ischemic tissue of brain
The supply of oxygen, there is provided nutriment necessary to metabolism simultaneously removes metabolic waste.Recover blood reperfusion in time to be beneficial to
Mitigate cerebral ischemia, at least make the recovery of some reversible lesions acquisitions functionally.
Transient ischemic attack is transient as caused by entocranial artery lesion or transience, focal brain or retina
Dysfunction, with the characteristics of the transience aphasia of recurrent exerbation, paralysis or sensory disturbance, break out last for several minutes every time, usually
Clinical symptoms continue 10-15 min, recover completely in 30 minutes, be no longer than 24 it is small when.Transient ischemic attack disease
Though shape is light, such as treat not in time, cerebral infarction will be developed into, threaten patient vitals.Therefore, it is usually clinically regarded as brain
The danger signal of angiosis.Ishemic stroke be due to the blood flow in certain artery blood supply area because of embolism or bleeding and temporarily or permanently
Caused by reduction, its pathologic process is complicated, and effective treatment means still lack at present, and treating transient ischemic attack in time is
Prevent the important measures of Ischemic Stroke.The diagnosis of transient ischemic attack there is no specific short at present.Western medical treatment is main
Using expansion blood vessel, improve microcirculation.Clinic is still based on thrombolysis, but there are the dangerous and other of bleeding in applying at present
Limitation.
The active principle extracted from natural plants often has extensive pharmacological action, such as improves blood circulation, protection
Cell caused by brain tissue confrontation cerebral ischemic injury, the effect of protection brain tissue anti-cerebral ischemia and encephaledema, prevention cerebral ischemia
The ion transport of film is disorderly, removes a variety of free radicals, mitigates the effect etc. of neonatal hypoxia ischemia infringement.But clinically Chinese medicine is independent
Herbal species for treating transient ischemic attack are less, and people not merely meet the therapeutic effect to these medicines.
The content of the invention
It is a kind of of short duration for preventing it is an object of the invention to design offer for the above-mentioned problems in the prior art
The technical solution of the natural plant extracts preparation of cerebral ischemia, its functional component content is high, easy to use and carry, oral absorption
Rapidly, bioavilability is high, prevention effect is obvious, and preparation process is simple, easy to operate, and working condition is easy to control, automation
Degree and production efficiency are high, green safe, can industrialized production, opened newly for prevention transient cerebral ischemia and using natural resources
Approach.
A kind of natural plant extracts preparation for being used to prevent transient cerebral ischemia, it is characterised in that by myrica ruba marc
Extract, plumage awns chrysanthemum general flavone, yeast selenium are according to weight ratio 50-80g:20-50 g:300-800 μ g are uniformly mixed；
The myrica ruba marc extract is prepared using following methods：By new arbutus, after stoning is squeezed the juice, centrifugal treating or
Using filter cloth parcel pomace extruding separation fruit juice and pomace；It is that then hydrous ethanol dosage is 3-6 to 1 kg according to myrica ruba marc weight
The ratio risen adds the ethanol solution of 55%-70%, and adds salt acid for adjusting pH 3.0-pH 4.0, soak extraction 2-3 times, every time
6-12 h, are extruded with filter cloth parcel pomace, obtain leaching liquor, with flash concentration device at 60-65 DEG C, vacuum 0.093-
The 1/3-1/6 of original volume is concentrated in vacuo under the conditions of 0.095 Mpa, obtains pomace concentrate；Pomace concentrate is inhaled by macropore
Attached resin Diaion HP 2MGL columns, the flow velocity of control 0.5-1.5 mL/min are used instead with after distillation water wash to water white transparency
The ethanol solution elution of the 50-60% of pH 3.0-4.0, eluent flow rate is 0.5-1.5 mL/min, collects coloured anthocyanidin
Eluent, dry or freeze-drying is concentrated in vacuo in Rotary Evaporators, obtains myrica ruba marc extract dry powder；Weigh and calculate
Rate, sample lucifuge kept dry, the yield of obtained myrica ruba marc extract are 0.57-1.56%, total anthocyanidin in extract
Content reaches 23.2-47.3%.
The plumage awns chrysanthemum general flavone is prepared using following methods：Dry plumage awns chrysanthemum herb is put to the appearance of smashing extractor
In device, it is that then hydrous ethanol dosage is that the ratio that 5-8 rises adds 50%-80% ethanol to 1 kg according to plumage awns chrysanthemum dry weight, opens
Dynamic switch, carries out room temperature texture with the rotating speed of 10000-12000 r/min and crushes extraction 2-3 times, raw material is broken into homogenate shape,
And the granularity of 40-60 mesh sieves can be passed through；Broken extraction time is 2-4 minutes every time, and extracting solution is filtered, discards filter residue, is merged
At 50 DEG C -70 DEG C, vacuum is carried out obtained extracting solution under the conditions of being 0.092-0.095 Mpa using Reduced Pressure Concentration Device
Flash concentration, is concentrated into the 1/5-1/8 of original volume, and concentrate then is continued vacuum drying into dry powder, finely ground with mortar
After obtain plumage awns chrysanthemum extractive of general flavone dry powder；The yield of extract reaches 15.2%-24.5%%, total in extract after measured
The content of flavones reaches 18.5-23.5%.
A kind of natural plant extracts preparation for being used to prevent transient cerebral ischemia, it is characterised in that myrica ruba marc carries
It is 55-75g to take thing, plumage awns chrysanthemum general flavone, the weight ratio of yeast selenium: 25-45 g: 400-700 μg.
A kind of natural plant extracts preparation for being used to prevent transient cerebral ischemia, it is characterised in that myrica ruba marc carries
It is 60-70g to take thing, plumage awns chrysanthemum general flavone, the weight ratio of yeast selenium: 30-40 g: 500-600 μg.
A kind of natural plant extracts preparation for being used to prevent transient cerebral ischemia, it is characterised in that the red bayberry
In pomace extract preparation method：It is that then hydrous ethanol dosage is that the ratio that 4-5 rises adds to 1 kg according to myrica ruba marc weight
The alcohol solution dipping extraction of 60%-65%, leaching liquor are concentrated in vacuo to the 1/4-1/5 of original volume；Pomace concentrate passes through macropore
The flow velocity for adsorbing resin Diaion HP 2MGL columns is 0.8-1.2 mL/min, uses the ethanol of the 52-55% of pH 3.2-3.5 instead
Solution elutes, and eluent flow rate is 0.8-1.2 mL/min.
A kind of natural plant extracts preparation for being used to prevent transient cerebral ischemia, it is characterised in that the plumage awns
In chrysanthemum general flavone preparation method：It is that then hydrous ethanol dosage is that the ratio that 6-7 rises adds to 1 kg according to plumage awns chrysanthemum dry weight
60%-70% ethanol, the extracting solution merged are concentrated into the 1/6-1/7 of original volume.
A kind of natural plant extracts preparation for being used to prevent transient cerebral ischemia, the preparation of corresponding buccal tablet,
Need to add the auxiliary materials such as appropriate Icing Sugar, cornstarch, sodium carboxymethylcellulose, magnesium stearate, ethanol as excipient, pass through
The processes such as the processing of auxiliary material, crushing, sieving, mixing, granulation, drying, tabletting, drying are completed；The preparation of corresponding granule formulation,
Need to add the auxiliary materials such as a small amount of starch and dextrin as excipient, add a small amount of wetting agent, pass through the processing of auxiliary material, system
The processes such as grain, drying, whole grain are completed；The preparation of capsule preparations is corresponded to, it is necessary to add a small amount of starch, will be upper using conventional method
The dry powder and auxiliary material for stating prescription are uniformly mixed and made into capsule；The preparation method of other formulations belongs to common process, no longer superfluous herein
State.
Red bayberry is the perennial green magaphanerophytes of Myruca ceas Myrica(Myrica).Red bayberry is quite big in the cultivated area of China, Zhejiang,
There are plantation in Jiangsu, Fujian each province, and using Zhejiang Province as most, its yield is huge for its area and quality etc., and only Zhejiang Province's annual output is just
Up to few hundred thousand tonnes of.Red bayberry contains a variety of materials beneficial to human body, and bayberry cyanidin is a kind of important function in Waxberry fruit
Property component, also known as anthocyanin, be widely present in the flower of chromophyte, fruit, anthocyan component is natural in food industry
The good source of food coloring, it was reported that there is bayberry cyanidin significant anti-oxidant, antifatigue, anti-aging to remove free radical
And other effects.
Plumage awns chrysanthemum plumage awns chrysanthemum is composite family long handle Chrysanthemum herbaceos perennial.Originate in american torrid zone area, it is rear propagate in
The states such as India, South East Asia Mainland and Indonesia.China introduces the southern various regions such as Hainan, Guangdong.Gan Wei, bitter cold.Plumage awns chrysanthemum is complete
Grass has the function that anti-oxidant, antibacterial anti-inflammatory etc..Research finds to contain substantial amounts of flavone compound in plumage awns chrysanthemum, it is known that
Flavone compound has the function that good anti-oxidant, suppression tumour, prevention cardiovascular and cerebrovascular disease etc..
With the development of science and technology effect of the trace elements of selenium to human health is also increasingly taken seriously.Organic selenium element can
Be widely used in produce selenium-enriched food, it to mankind's anti-aging, promote longevity, for anti-curing cancers, myopia, cataract, the heart
The diseases such as cerebrovascular disease, endemic disease have all played certain effect.In food and production, it is widely used organic selenium product and makees
For the replenishers of trace elements of selenium.Some developed countries produce and are had reached using selenium and other trace elements preparation suitable at present
Scale.Organic selenium product, including the healthy nutritive value of selenium enriched Spirulina, selenium yeast etc. is to be difficult to substitute in product of the same trade
's.
A kind of above-mentioned natural plant extracts preparation for being used to prevent transient cerebral ischemia, has the following advantages：
1）Myrica ruba marc is as discarded object, and resource reutilization cost is low, and drug effect value is high；Plumage awns chrysanthemum aboundresources, source
Easily；
2）Natural plant extracts preparation prepared by the present invention, functional component content is high, easy to use and carry, takes orally and inhales
It is obvious to receive rapid, bioavilability height, prevention effect, and is proven by animal experiment；
3）Preparation process of the present invention is simple, easy to operate, working condition is easy to control, the degree of automation and production efficiency
Height, it is green safe, prevention effect significantly, can industrialized production, opened newly for prevention transient cerebral ischemia and using natural resources
Approach.
Percentage composition involved in present patent application unless otherwise indicated outer, remaining is the pure material of active ingredient
Weight percentage.
Embodiment
Beneficial effects of the present invention are further illustrated below in conjunction with specific embodiment and corresponding test data.
Embodiment 1
This is used for the natural plant extracts preparation for preventing transient cerebral ischemia, always yellow by myrica ruba marc extract, plumage awns chrysanthemum
Ketone, yeast selenium are according to weight ratio 50g: 50 g:600 μ g are uniformly mixed；
The myrica ruba marc extract is prepared using following methods：By new arbutus, after stoning is squeezed the juice, centrifugal treating or
Using filter cloth parcel pomace extruding separation fruit juice and pomace；It is that then hydrous ethanol dosage is 3 liters to 1 kg according to myrica ruba marc weight
Ratio add 55% ethanol solution, and add salt acid for adjusting pH 4.0, soak extraction 3 times, 12 h, is wrapped up with filter cloth every time
Pomace extrudes, and obtains leaching liquor, and with flash concentration device at 60 DEG C, vacuum is concentrated in vacuo to original under the conditions of being 0.095 Mpa
The 1/6 of volume, obtains pomace concentrate；Pomace concentrate is passed through into macroporous absorbent resin Diaion HP 2MGL columns, control
1.0mL/min flow velocity with distillation water wash to water white transparency after, use instead pH 3.5 60% ethanol solution elution, eluent
Flow velocity is 1.0 mL/min, collects coloured anthocyanidin eluent, and dry or freeze-drying is concentrated in vacuo in Rotary Evaporators,
Myrica ruba marc extract dry powder, weigh and calculate yield, sample lucifuge kept dry, the receipts of obtained myrica ruba marc extract
Rate is 1.35%, and the content of total anthocyanidin reaches 32.3% in extract.
The plumage awns chrysanthemum general flavone is prepared using following methods：Dry plumage awns chrysanthemum herb is put to the appearance of smashing extractor
In device, according to plumage awns chrysanthemum dry weight be the 1 kg ratios that then hydrous ethanol dosage is 6 liters add 75% ethanol, starting switch,
Room temperature texture is carried out with the rotating speed of 10000 r/min and crushes extraction 2 times, raw material is broken into homogenate shape, and 60 mesh sieves can be passed through
Granularity；Broken extraction time is 3 minutes every time, and extracting solution is filtered, discards filter residue, the extracting solution merged at 50 DEG C,
Vacuum uses Reduced Pressure Concentration Device to carry out flash concentration under the conditions of being 0.095 Mpa, is concentrated into the 1/6 of original volume, so
Concentrate is continued into vacuum drying into dry powder afterwards, with mortar it is finely ground after obtain plumage awns chrysanthemum extractive of general flavone dry powder, extract
The yield of thing reaches 22.1%, and the content of general flavone reaches 19.9% in extract after measured.
Embodiment 2
This is used for the natural plant extracts preparation for preventing transient cerebral ischemia, always yellow by myrica ruba marc extract, plumage awns chrysanthemum
Ketone, yeast selenium are according to weight ratio 60g: 40 g:500 μ g are uniformly mixed, the other the same as in Example 1；
The myrica ruba marc extract is prepared using following methods：By new arbutus, after stoning is squeezed the juice, centrifugal treating or
Using filter cloth parcel pomace extruding separation fruit juice and pomace；It is that then hydrous ethanol dosage is 6 liters to 1 kg according to myrica ruba marc weight
Ratio add 70% ethanol solution, and add salt acid for adjusting pH 3.0, soak extraction 2 times, each 10h, fruit is wrapped up with filter cloth
Slag extrudes, and obtains leaching liquor, and with flash concentration device at 60 DEG C, vacuum is concentrated in vacuo to substance under the conditions of being 0.095 Mpa
Long-pending 1/5, obtains pomace concentrate；By pomace concentrate by macroporous absorbent resin Diaion HP 2MGL columns, 1.5mL/ is controlled
The flow velocity of min uses the 50% ethanol solution elution of pH 3.0 instead with after distillation water wash to water white transparency, and eluent flow rate is
1.5 mL/min, collect coloured anthocyanidin eluent, and dry or freeze-drying is concentrated in vacuo in Rotary Evaporators, obtains red bayberry
Pomace extract dry powder, weighs and calculates yield, sample lucifuge kept dry, the yield of obtained myrica ruba marc extract is
1.41%, the content of total anthocyanidin reaches 36.7% in extract.
The plumage awns chrysanthemum general flavone is prepared using following methods：Dry plumage awns chrysanthemum herb is put to the appearance of smashing extractor
In device, according to plumage awns chrysanthemum dry weight be the 1 kg ratios that then hydrous ethanol dosage is 7 liters add 60% ethanol, starting switch,
Room temperature texture is carried out with the rotating speed of 10000 r/min and crushes extraction 2 times, raw material is broken into homogenate shape, and 40 mesh sieves can be passed through
Granularity；Broken extraction time is 2.5 minutes every time, and extracting solution is filtered, discards filter residue, the extracting solution merged is 60
DEG C, vacuum uses Reduced Pressure Concentration Device to carry out flash concentration under the conditions of being 0.095 Mpa, is concentrated into the 1/7 of original volume,
Then concentrate is continued into vacuum drying into dry powder, with mortar it is finely ground after obtain plumage awns chrysanthemum extractive of general flavone dry powder, carry
The yield of thing is taken to reach 18.3%%, the content of general flavone reaches 19.6% in extract after measured.
Embodiment 3
This is used for the natural plant extracts preparation for preventing transient cerebral ischemia, always yellow by myrica ruba marc extract, plumage awns chrysanthemum
Ketone, yeast selenium are according to weight ratio 70g: 30 g:800 μ g are uniformly mixed, the other the same as in Example 1；
The myrica ruba marc extract is prepared using following methods：By new arbutus, after stoning is squeezed the juice, centrifugal treating or
Using filter cloth parcel pomace extruding separation fruit juice and pomace；It is that then hydrous ethanol dosage is 5 liters to 1 kg according to myrica ruba marc weight
Ratio add 60% ethanol solution, and add salt acid for adjusting pH 3.5, soak extraction 3 times, each 8h, fruit is wrapped up with filter cloth
Slag extrudes, and obtains leaching liquor, and with flash concentration device at 60 DEG C, vacuum is concentrated in vacuo to substance under the conditions of being 0.095 Mpa
Long-pending 1/6, obtains pomace concentrate；By pomace concentrate by macroporous absorbent resin Diaion HP 2MGL columns, 1.0mL/ is controlled
The flow velocity of min uses the 50% ethanol solution elution of pH4.0 instead with after distillation water wash to water white transparency, and eluent flow rate is
1.0 mL/min, collect coloured anthocyanidin eluent, and dry or freeze-drying is concentrated in vacuo in Rotary Evaporators, obtains red bayberry
Pomace extract dry powder, weighs and calculates yield, sample lucifuge kept dry, the yield of obtained myrica ruba marc extract is
1.54%, the content of total anthocyanidin reaches 35.7.3% in extract.
The plumage awns chrysanthemum general flavone is prepared using following methods：Dry plumage awns chrysanthemum herb is put to the appearance of smashing extractor
In device, according to plumage awns chrysanthemum dry weight be the 1 kg ratios that then hydrous ethanol dosage is 5 liters add 65% ethanol, starting switch,
Room temperature texture is carried out with the rotating speed of 10000 r/min and crushes extraction 3 times, raw material is broken into homogenate shape, and 60 mesh sieves can be passed through
Granularity；Broken extraction time is 4 minutes every time, and extracting solution is filtered, discards filter residue, the extracting solution merged at 60 DEG C,
Vacuum uses Reduced Pressure Concentration Device to carry out flash concentration under the conditions of being 0.095 Mpa, is concentrated into the 1/5 of original volume, so
Concentrate is continued into vacuum drying into dry powder afterwards, with mortar it is finely ground after obtain plumage awns chrysanthemum extractive of general flavone dry powder, extract
The yield of thing reaches 17.6%, and the content of general flavone reaches 21.9% in extract after measured.
Below in conjunction with corresponding experiment, the invention will be further described.
Influences of the 1-3 of the embodiment of the present invention to mouse transient cerebral ischemia re-perfusion model
Cleaning grade male Kunming strain mice, weight 18-22g, is provided by Zhejiang Academy of Medical Sciences, experimental situation temperature
23-24 degree, humidity 56%-59%.Nitric oxide kit, Malondialdehyde Kit, superoxide dismutase kit, coomassie are bright
Blue kit is purchased from builds up bio tech ltd in Nanjing.
Mouse freely ingests and drinks water, and is adapted to 3 days before testing, and formal experiment is entered after check is qualified.Mouse is randomly divided into 6
Group, is respectively control group（Sham-operation group）, model group, positive group, the high, medium and low dosage group of medicine（6g/kg、3g/kg、1.5g/
kg）, every group 10.Medicine and Nimodipine are made into suspension with physiological saline before use, respectively at fill again 0 it is small when and 6 it is small when
Gastric infusion is once.Each group is taken orally by 0.2ml/10g give test medicine respectively, and control group and model group are injected during administration
Deng dosage physiological saline, positive group injects Nimodipine when being administered（30mg/kg/24h）, one time a day.Continuous gavage is administered 7 days,
Mouse is weighed after last dose 1h, afterwards with 3.5% chloraldurate intraperitoneal injection of anesthesia mouse, is faced upward and is fixed on mouse fixing device,
75% alcohol disinfecting of neck, using the separation total artery of bilateral strength, blocks bilateral common carotid arteries blood flow 30min blocking blood flow methods,
Mouse transient cerebral ischemia model is caused, is sutured after rear recovery perfusion, after each mouse recovers Reperfu- sion 24h, cervical dislocation is put to death, fast
Speed takes out brain tissue and weighs.Control group only rehearses surgical procedure, not blocking blood flow, and after 24h, cervical dislocation is put to death, and broken end takes out
Brain tissue is weighed.Brain tissue is placed in homogenizer, with ice physiological saline by quality and volume ratio 1:9 ratio, is made volume
Fraction is 10% brain tissue homogenate, measures protein content in brain tissue using Coomassie Brilliant Blue, while build up life according to Nanjing
The kit specification that thing Graduate School of Engineering provides, using an oxygen in microplate reader and ultraviolet-uisible spectrophotometer measure brain tissue
Change the content of nitrogen, superoxide dismutase and malonaldehyde.
Brain tissue determining the protein quantity：Brain tissue homogenate is taken, with physiological saline 1:4 are diluted to the homogenate of 2% tissue, according to egg
White quantitative test box specification operation, measures brain tissue protein content using ultraviolet specrophotometer at 595nm, the results are shown in Table
1.After transient cerebral ischemia, brain albumen tissue content obvious reduction of the model group mouse than sham-operation group mouse.By one week
Prevention administration, compared with model group mouse, after transient cerebral ischemia, the brain tissue protein content of administration group significantly improves, medicine
The high, medium and low dosage group of thing has significant difference (P<0.01).The wherein brain tissue content highest of high dose group.Experimental result is said
Bright, embodiment 1-3 has mouse transient cerebral ischemia re-perfusion model certain prevention effect, and with the linear pass of concentration
System.The preventive effect of wherein high dose group is best, can significantly improve the content of albumen in Mice brain tissues.
Content assay of nitric oxide：10% brain tissue homogenate's liquid is taken, adds color developing agent according to nitric oxide testing cassete specification
（Standard items sodium nitrite titer）, mix, stand 15min, with microplate reader in 550nm colorimetric estimations, calculate an oxidation
The content of nitrogen.The mouse of model group passes through transient cerebral ischemia, and content of nitric oxide substantially increases compared with sham-operation group, by
After the mouse brain Temporary ischemia of medicine prevention, content of nitric oxide substantially reduces, and with the increase of drug dose, nitric oxide
Content reduces more very, with drug dose inversely.Wherein, the brain tissue content of nitric oxide of medicine high dose group is minimum,
Model group mouse than no administration reduces clearly.The experiment results show that embodiment 1-3 is to mouse transient cerebral ischemia
Re-perfusion model has certain prevention effect, and linear with concentration.The preventive effect of wherein high dose group is best, can
To significantly reduce content of nitric oxide in Mice brain tissues.
Superoxide dismutase（SOD）Assay：In many factors of cerebral ischemia re-pouring injured mechanism, oxygen is certainly
Played an important role wherein by the generation of base.Free radical produces the excessive peroxidating that can cause lipid, protein and nucleic acid, makes
Membrane structure is destroyed, and cell occurs irreversible finally death, the microcirculation disorder and blood-brain barrier for causing brain tissue of changing and leads to
Permeability increase.SOD is the enzyme of main removing ultra-oxygen anion free radical in vivo, has Scavenger of ROS ROS, including super oxygen the moon
The functions such as ion radical, its activity reduction can cause a large amount of free radical accumulations of intracerebral, cause the 26S Proteasome Structure and Function of cell membrane to meet with
To destruction, neuronal damage.
10% brain tissue homogenate's liquid is taken, matches somebody with somebody solution to be measured, 450nm according to superoxide dismutase reagent examination box specification
Locate microplate reader measure, be shown in Table 3.After experiment is not as can be seen that do the mouse transient cerebral ischemia of any precautionary measures, mould
The brain tissue SOD contents of type group mouse substantially reduce, about 60% is reduced than normal sham-operation group, and have particularly significant property meaning
(P<0.01).And have by the SOD contents being administered in the Mice brain tissues for the medicine group prevented significantly increase before, and with
The increase of drug dose, SOD contents are consequently increased.Wherein, SOD contents are obvious in high and middle dose group Mice brain tissues
Increase, and there is significant difference (P<0.01).Illustrate that medicine group can effectively improve superoxide dismutase in Mice brain tissues
Enzyme content, has obvious prevention effect to mouse transient cerebral ischemia re-perfusion model,.The wherein high and prevention of middle dose group
Effect is preferable, can significantly improve the situation of mouse transient cerebral ischemia Reperfu- sion.
Malonaldehyde（MDA）Assay：10% brain tissue homogenate's liquid is taken, is configured according to malonaldehyde testing cassete specification each
Solution, mixes, and 95 DEG C of water-baths 40 minutes, take out postcooling, be then centrifuged for 10 minutes.Supernatant is taken, uses ultraviolet specrophotometer
Absorbance is surveyed at 532nm, MDA contents is calculated, is shown in Table 4.Experiment is found, is lacked without the mouse of precautionary measures in transience brain
After blood, the mda content in brain tissue is all increased significantly, i.e. the MDA contents of model group apparently higher than normal sham-operation group,
And there is significant difference (P<0.01).And the medicine group after prevention administration is after transient cerebral ischemia, MDA in brain tissue
Content substantially reduces, and as drug concentration increases, MDA contents gradually reduce.Illustrate that embodiment 1-3 lacks mouse transience brain
Blood re-perfusion model has obvious prevention effect, can significantly improve the situation of mouse transient cerebral ischemia Reperfu- sion.
Claims (5)
1. a kind of be used to prevent the natural plant extracts preparation of transient cerebral ischemia, it is characterised in that by myrica ruba marc extract,
Plumage awns chrysanthemum general flavone, yeast selenium are according to weight ratio 50-80g:20-50 g:300-800 μ g are uniformly mixed；
The myrica ruba marc extract is prepared using following methods：By new arbutus, after stoning is squeezed the juice, centrifugal treating or use
Filter cloth parcel pomace extruding separation fruit juice and pomace；It is that then hydrous ethanol dosage is what 3-6 rose to 1 kg according to myrica ruba marc weight
Ratio adds the ethanol solution of 55%-70%, and adds salt acid for adjusting pH 3.0-pH 4.0, soak extraction 2-3 times, each 6-12
H, is extruded with filter cloth parcel pomace, obtains leaching liquor, with flash concentration device at 60-65 DEG C, vacuum 0.093-0.095
The 1/3-1/6 of original volume is concentrated in vacuo under the conditions of Mpa, obtains pomace concentrate；Pomace concentrate is passed through into macroporous absorbent resin
Diaion HP 2MGL columns, the flow velocity of control 0.5-1.5 mL/min use pH 3.0- instead with after distillation water wash to water white transparency
The ethanol solution elution of 4.0 50-60%, eluent flow rate is 0.5-1.5 mL/min, collects coloured anthocyanidin eluent,
Dry or freeze-drying is concentrated in vacuo in Rotary Evaporators, obtains myrica ruba marc extract dry powder；
The plumage awns chrysanthemum general flavone is prepared using following methods：Dry plumage awns chrysanthemum herb is put to the container of smashing extractor
In, it is that then hydrous ethanol dosage is that the ratio that 5-8 rises adds 50%-80% ethanol to 1 kg according to plumage awns chrysanthemum dry weight, starts
Switch, carries out room temperature texture with the rotating speed of 10000-12000 r/min and crushes extraction 2-3 times, raw material is broken into homogenate shape, and
The granularity of 40-60 mesh sieves can be passed through；Broken extraction time is 2-4 minutes every time, and extracting solution is filtered, discards filter residue, is merged
The extracting solution arrived at 50 DEG C -70 DEG C, under the conditions of being 0.092-0.095 Mpa using Reduced Pressure Concentration Device dodged by vacuum
Inspissation contracts, and is concentrated into the 1/5-1/8 of original volume, and concentrate then is continued vacuum drying into dry powder, with mortar it is finely ground after
Obtain plumage awns chrysanthemum extractive of general flavone dry powder.
A kind of 2. natural plant extracts preparation for being used to prevent transient cerebral ischemia as claimed in claim 1, it is characterised in that
Myrica ruba marc extract, plumage awns chrysanthemum general flavone, the weight ratio of yeast selenium are 55-75g: 25-45 g: 400-700 μg.
A kind of 3. natural plant extracts preparation for being used to prevent transient cerebral ischemia as claimed in claim 1, it is characterised in that
Myrica ruba marc extract, plumage awns chrysanthemum general flavone, the weight ratio of yeast selenium are 60-70g: 30-40 g: 500-600 μg.
A kind of 4. natural plant extracts preparation for being used to prevent transient cerebral ischemia as claimed in claim 1, it is characterised in that
In the myrica ruba marc method for preparing extractive：It is that then hydrous ethanol dosage is what 4-5 rose to 1 kg according to myrica ruba marc weight
Ratio adds the alcohol solution dipping extraction of 60%-65%, and leaching liquor is concentrated in vacuo to the 1/4-1/5 of original volume；Pomace concentrate
Flow velocity by macroporous absorbent resin Diaion HP 2MGL columns is 0.8-1.2 mL/min, uses the 52- of pH 3.2-3.5 instead
55% ethanol solution elution, eluent flow rate is 0.8-1.2 mL/min.
A kind of 5. natural plant extracts preparation for being used to prevent transient cerebral ischemia as claimed in claim 1, it is characterised in that
In the plumage awns chrysanthemum general flavone preparation method：It is that then hydrous ethanol dosage is what 6-7 rose to 1 kg according to plumage awns chrysanthemum dry weight
Ratio adds 60%-70% ethanol, and the extracting solution merged is concentrated into the 1/6-1/7 of original volume.
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| CN105504080B (en) * | 2015-12-15 | 2018-01-26 | 武汉华纳联合药业有限公司 | Myricanol soluble polysaccharide and its production and use |
| CN105535766A (en) * | 2016-01-07 | 2016-05-04 | 陈彬 | Traditional Chinese medicine for treating kidney essence deficiency type transient ischemic attack and preparation method |
| CN114657115A (en) * | 2020-12-23 | 2022-06-24 | 科索瑞生物科技(天津)有限公司 | Animal umbilical cord tissue extract and preparation method and application thereof |
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Effective date of registration: 20230531 Address after: No. 196, Lujia Village, Ciwu Town, Zhuji City, Shaoxing City, Zhejiang Province, 311800 Patentee after: Zhejiang Yutai Construction Leasing Co.,Ltd. Address before: 311800 No.77 Puyang Road, Zhuji City, Shaoxing City, Zhejiang Province Patentee before: JIYANG COLLEGE OF ZHEJIANG A&F University |
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Denomination of invention: A natural plant extract preparation for preventing and treating transient cerebral ischemia Effective date of registration: 20230719 Granted publication date: 20180417 Pledgee: Shaoxing Bank Co.,Ltd. Zhuji sub branch Pledgor: Zhejiang Yutai Construction Leasing Co.,Ltd. Registration number: Y2023980048971 |