CN103169742A - Application of elaeagnus conferta roxb - Google Patents

Application of elaeagnus conferta roxb Download PDF

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CN103169742A
CN103169742A CN2013100999707A CN201310099970A CN103169742A CN 103169742 A CN103169742 A CN 103169742A CN 2013100999707 A CN2013100999707 A CN 2013100999707A CN 201310099970 A CN201310099970 A CN 201310099970A CN 103169742 A CN103169742 A CN 103169742A
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elaeagnus conferta
conferta fruits
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cholelithiasis
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盛艳
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Abstract

The invention discloses an application of elaeagnus conferta roxb, namely the application of the elaeagnus conferta roxb in preparation of drugs for treating kidney stones, gall-stone and hyperuricemia. In the treatment of the kidney stones, the drug is used for reducing the rate of the kidney stones, improving kidney coefficient, reducing calcium content of the kidney, reducing serum urea nitrogen and reducing serum creatinine. In the treatment of the gall-stone, the elaeagnus conferta roxb is used for treating bile pigment gallstones and cholesterol gallstones; preferably in the treatment of bilirubin gallstones, the elaeagnus conferta roxb is used for reducing the rate of the gallbladder stones, calcium ion content in bile, total bilirubin concentration and free bilirubin concentration in the treatment of the bile pigment gallstones; in the treatment of the cholesterol gallstones, the elaeagnus conferta roxb is used for reducing the rate of the gallbladder stones and the cholesterol content in the bile; and in the treatment of hyperuricemia, the elaeagnus conferta roxb is used for reducing uric acid content in serum. The application of the elaeagnus conferta roxb can be spread to related products of the elaeagnus conferta roxb; and the related product includes a fruit aqueous extract of the elaeagnus conferta roxb, dewatered elaeagnus conferta roxb, capsules, troches, granulas, fruit vinegar, jam and fruit juice.

Description

A kind of purposes of elaeagnus conferta fruits
Technical field
The present invention relates to a kind of purposes of elaeagnus conferta fruits, specifically, the present invention relates to a kind of elaeagnus conferta fruits for the preparation of the application in the medicine for the treatment of renal calculus, cholelithiasis and hyperuricemia, belong to the field of Chinese medicines.
Background technology
Elaeagnus conferta fruits (Elaeagnus conferta Roxb), have another name called close colored Fructus Elaeagni, southern Fructus Elaeagni, large fruit Fructus Elaeagni, rattan Fructus Elaeagni, confused fruit, Ma Luanguo, be a kind of wild medicine food dual purpose plant, be distributed widely in Geothermal Belt rainforest and Yunnan Province of China south and the south Guangxi such as Vietnam, Malaysia, India.Its nutritional labeling is very abundant.It is reported, contain vitamin C 12～30mg in every 100g fresh fruit, carotene 3.15mg, vitamin B 0.4～0.7mg; Sarcocarp is sugary 5.11%, total acid 1.45%, crude protein 2.46%, crude fat 2.88%; Contain various trace elements, the content of potassium, sodium, calcium, copper, ferrum, zinc etc. is apparently higher than the fruit of great majority cultivation; Contain 17 seed amino acids, have 7 kinds more than in the aminoacid of 8 kinds of needed by human, and content is all higher.
Meanwhile, elaeagnus conferta fruits has higher medical value.Modern pharmacology studies have shown that, elaeagnus conferta fruits plant root is bitter flat, expelling wind and removing dampness, the blood stasis expelling hemostasis, to infectious hepatitis, rheumatic arthritis, infantile malnutrition, spitting of blood, spit blood, have blood in stool, metrorrhagia, leucorrhea, traumatic injury etc. are effective in cure; The little hardship of leaf, flat, relieving cough and asthma, effective in cure to bronchitis, cough, asthma etc.; Fruit is sweet, sour, flat, and the dysentery relieving that helps digestion has therapeutical effect to enteritis, dysentery, inappetence etc.
Lithiasis is one of urinary system common disease, refers to betide the calculus of renal calices, renal pelvis and renal pelvis and ureter connecting portion.The calculus at any position of urinary system all can be primary in kidney.According to the difference of stone composition, renal calculus can be divided calcium oxalate stone, calcium phosphate stone, uric acid (urate) calculus, struvite calculus, cystine stone and purine calculus six classes.Its formation is subject to the impact of factors, comprises natural environment, social life condition, dietary structure custom, the local illness of urinary system, general metabolism disorder.Clinical manifestation for morbidity suddenly, violent lumbago, pain is persistence or intermittence more, and along ureter to iliac fossa, perineum and scrotum etc. locate radiation and hematuria or pyuria occur, dysuria or urine stream interrupt etc.Be more common in the male, directly affect renal function, even cause the kidney merit to decline, endanger very large.
Along with global warming in recent years, eating condition improves, and the sickness rate of renal calculus is increasing year by year.In the U.S., the annual morbidity of renal calculus is 13%, and Canada is 12%, and the sickness rate of Saudi Arabia's renal calculus has surpassed 20%.The incidence rate of China's urinary system calculus is 1-5%, and is southern up to 5-10%, year neopathy rate 150-200/10 ten thousand people.At present clinical take Extracorporeal shock wave lithotripsy (ESWL) or other calculus technology more.Though this method can make calculus discharge, side effect is very many, and the residual rate of calculus and relapse rate very high.According to statistics, the risk of relapse of renal calculus reaches to reach in 67%, 25 year in 50%, 9 year in 5 years and reaches 75%.Therefore seek a kind of efficiently and effectively therapeutic modality very necessary.
Cholelithiasis is also one of China's commonly encountered diseases, frequently-occurring disease, has a strong impact on people's healthy, working and learning, and is very high at surgery mortality rate clinically.It mainly comprises cholelithiasis, choledocholithiasis, calculus of intrahepatic duct.The sickness rate of this disease in population of China is about 15%.The composition of cholelithiasis comprises the trace element such as cholesterol, bilirubin, bile acid, phospholipid, protein and calcium, ferrum, zinc, copper, magnesium, potassium, phosphorus.
Mankind's cholelithiasis can be divided into cholesterol calculus, bile pigment stone and mixed type calculus three classes by its chief component composition.90% bile pigment calculus refers to bilirubin calculus.The mixed type cholelithiasis refers to be by cholesteric layer, bile pigments layer and layers of calcium salts and periodically is bundled together the cholelithiasis that consists of.
Modern medicine treatment cholelithiasis adopts operative treatment mostly, but remnant gallstone rate and the relapse rate of cutting gallbladder or bile duct lithotomy are higher, and cholecystectomy has considerable influence to patient's digestive function.Extracorporeal shock-wave lithotripsy (ESWL) technology curative effect still can, but risk is larger.Non-operative treatment to be adopted oral and the direct litholytic therapy of perfusion, but the course for the treatment of length, side effect is many and expensive.New approach has been opened up in developing rapidly as the interventional therapy of cholelithiasis of therapeutic endoscopy technology, and the restriction that still is subject to again the factors such as technology, appointed condition is difficult to promote.Therefore, try to explore that therapeutic modality is imperative safely and effectively.
In recent years, along with improving constantly of living standard, the sickness rate of hyperuricemia and gout is also raising year by year.Hyperuricemia refers to continue the disease of 〉=416 μ mol/L (7.0mg/dl) because a variety of causes causes serum Uric Acid Concentration.Serum Uric Acid Concentration depends on the balance between uricopoiesis speed and drainage rate, and uricopoiesis increases or/and drain minimizing all can cause hyperuricemia.It is the biochemical basis of goat.Over the supersaturated concentration in its blood, can form urate deposition when hyperuricemia in tissue, cause gout to occur.The gout clinical manifestation is gouty arthritis (GA), gouty nephropathy, gout calculus etc.Gout must be with hyperuricemia.Approximately the 5%-12% Patients with Hyperuricemia can develop into gout.
Sex, age, diet and drink, occupation all can affect the morbidity of hyperuricemia and gout with environment, schooling, individual's intelligence and social status etc.These two kinds of diseases in the west and Japan become already commonly encountered diseases, they also became the disease prevailing of China (especially coastal open area) in recent years, and age of onset becomes younger gradually.Be mainly the high purines food of restriction to the prevention of hyperuricemia and gout at present, control is drunk.Main chemotherapy, for example benzbromarone (promotion urate excretion) and the allopurinol (inhibition uricopoiesis) of adopting in treatment.But these adverse effectes are more, and benzbromarone can cause serious hepatic injury and nervous system untoward reaction, and allopurinol can cause skin allergy, hepatic injury, super quick allergy syndrome, but life-threatening when serious.
Summary of the invention
For the defective of prior art, the object of the present invention is to provide a kind of purposes of elaeagnus conferta fruits, specifically described purposes is that elaeagnus conferta fruits is for the preparation of the application of the medicine for the treatment of renal calculus, cholelithiasis and hyperuricemia.
The objective of the invention is to be achieved through the following technical solutions.
A kind of purposes of elaeagnus conferta fruits, described purposes are that elaeagnus conferta fruits is for the preparation of the application of the medicine for the treatment of renal calculus, cholelithiasis and hyperuricemia.
Wherein, described treatment renal calculus refers to reduce the kidney stone rate, improves Kidney coefficients, reduces the kidney calcium content, reduces serum urea nitrogen and reduces serum creatinine.
The treatment cholelithiasis refers to treat bilins cholelithiasis and cholesterol cholelithiasis.
Preferred therapeutic bilirubinoid cholelithiasis in treatment bilins cholelithiasis, described treatment bilirubinoid cholelithiasis refers to reduce calcium ion content, total bilirubin concentration and the unconjugated bilirubin concentration in cholelithiasis rate, bile.
Treatment cholesterol cholelithiasis refers to reduce the cholesterol level in cholelithiasis rate and bile.
The treatment hyperuricemia refers to reduce serum uric acid.
The purposes of elaeagnus conferta fruits of the present invention, wherein verify by following experiment for the preparation of the application of the medicine for the treatment of renal calculus:
Cause the stone feedstuff to the rat renal calculus of feeding, the elaeagnus conferta fruits of feeding simultaneously, abdominal aortic bloods after continuous eight weeks detect blood urea nitrogen, creatinine content, get kidney and detect Kidney coefficients, get left kidney and survey the kidney calcium content, and right kidney is done pathological examination, surveys gallstone formation rate.Result: elaeagnus conferta fruits and elaeagnus conferta fruits product can significantly reduce the kidney stone rate, improve Kidney coefficients, reduce kidney calcium content, serum urea nitrogen and serum creatinine.Conclusion: elaeagnus conferta fruits can be used in the medicine of preparation treatment renal calculus.
The purposes of elaeagnus conferta fruits of the present invention, wherein verify by following experiment for the preparation of the application of the medicine for the treatment of the bilirubinoid cholelithiasis:
Cause the stone feedstuff to the Cavia porcellus bilirubinoid cholelithiasis of feeding, the elaeagnus conferta fruits of feeding is simultaneously put to death Cavia porcellus after continuous eight weeks, takes off gallbladder, extracts bile and measures calcium ion, total bilirubin, conjugated bilirubin, and calculate unconjugated bilirubin concentration.Cut gallbladder open and calculate the gallbladder gallstone formation rate.Result: elaeagnus conferta fruits can significantly reduce the cholelithiasis rate, the calcium ion content in bile, total bilirubin concentration, unconjugated bilirubin concentration.Conclusion: elaeagnus conferta fruits can be used in the medicine of preparation treatment bilirubinoid cholelithiasis.
The purposes of elaeagnus conferta fruits of the present invention, wherein verify by following experiment for the preparation of the application of the medicine for the treatment of the cholesterol cholelithiasis:
Cause the stone feedstuff to the Cavia porcellus cholesterol cholelithiasis of feeding, the elaeagnus conferta fruits of feeding is simultaneously put to death Cavia porcellus after continuous eight weeks, takes off gallbladder, extracts bile and measures cholesterol, bile acid and calcium ion concentration.Cut gallbladder open and calculate the gallbladder gallstone formation rate.Result: elaeagnus conferta fruits can significantly reduce calcium ion and the cholesterol concentration in calculus rate, bile.Conclusion: elaeagnus conferta fruits can be used in the medicine of preparation treatment cholesterol cholelithiasis.
The purposes of elaeagnus conferta fruits of the present invention, wherein verify by following experiment for the preparation of the application of the medicine for the treatment of hyperuricemia:
Give the rats by intraperitoneal injection hypoxanthine, the elaeagnus conferta fruits of feeding simultaneously, after continuous eight weeks, the eye socket rear vein beard is taken a blood sample, and detects assay kit with uric acid and measures Level of Serum Uric Acid.Result: elaeagnus conferta fruits can significantly reduce serum uric acid concentration.Conclusion: elaeagnus conferta fruits can be used in the medicine of preparation treatment hyperuricemia.
The purposes of elaeagnus conferta fruits of the present invention can also extend to the Related product of elaeagnus conferta fruits, comprises elaeagnus conferta fruits fruit water extract, dehydration elaeagnus conferta fruits, capsule, tablet, granule, fruit vinegar, fruit jam and fruit juice.
The specific embodiment
In order to prove absolutely characteristic of the present invention and to implement mode of the present invention, the below provides embodiment.
Embodiment 1
Elaeagnus conferta fruits treatment renal calculus
Experimental technique:
Get 50 of Wistar rats (male, body weight 180-220g, the SPF level, Chinese pharmaceutical biological product identifies that institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.Each is organized rat and all uses normal diet and distilled water to raise.Model group, positive controls, high dose group, four groups of rats of low dose group are only used 1% ethylene glycol (EG)+2% ammonium chloride (AC) gavage every day 2mL/.It is administration that modeling begins, every day 1 time, continuous 8 weeks.Positive control group selection Chinese medicine granules for treating nephrolith is as positive control drug, every day gavage 3g/kg.The fragmentation of elaeagnus conferta fruits fruit is made suspension with 0.5% sodium carboxymethyl cellulose solution afterwards.High dose group gavage every day elaeagnus conferta fruits 10g/kg; Low dose group gavage every day elaeagnus conferta fruits 2g/kg.After last administration 1h, use the pentobarbital sodium anesthetized animal, abdominal aortic blood detects blood urea nitrogen, creatinine content, gets kidney and detects Kidney coefficients, gets left kidney and surveys the kidney calcium content, and right kidney is done pathological examination, surveys gallstone formation rate.Experimental result is used the SPSS statistical software.Measurement data with
Figure BSA00000869439000041
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the gallstone formation rate of model group is significantly higher than matched group, and Kidney coefficients is significantly lower than matched group, and kidney calcium content, serum urea nitrogen and serum creatinine are significantly higher than matched group, prompting stone model modeling success.High dose group can significantly reduce the calculus rate, improves Kidney coefficients, reduces kidney calcium content, serum urea nitrogen and serum creatinine.Experimental result sees Table 1,2.
The impact of table 1 elaeagnus conferta fruits on the kidney of rats calculus
Figure BSA00000869439000051
The impact of table 2 elaeagnus conferta fruits on large mouse with renal calculs Kidney coefficients, kidney calcium content, serum creatinine and serum urea nitrogen
Figure BSA00000869439000052
Figure BSA00000869439000053
Annotate: compare with model group, *P＜0.05, *P＜0.01
Elaeagnus conferta fruits treatment bilirubinoid cholelithiasis
Experimental technique:
Get 50 of Cavia porcelluss (female, body weight 250-300g cleans level, and Chinese pharmaceutical biological product identifies that institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.The matched group Cavia porcellus is raised and uses normal diet, all the other are respectively organized the Cavia porcellus raising and use the bilirubinoid cholelithiasis to cause the stone feedstuff, and filling a prescription is: normal diet 95.43%, casein 1%, sucrose 1.5%, Adeps Sus domestica 1%, cellulose 1%, cholic acid 0.02%, cholesterol 0.05%.It is administration that modeling begins, every day 1 time, continuous 8 weeks.Positive control group selection Chinese medicine Fangshi capsule is as positive control drug, every day gavage 3g/kg.The fragmentation of elaeagnus conferta fruits fruit is made suspension with 0.5% sodium carboxymethyl cellulose solution afterwards.High dose group gavage every day elaeagnus conferta fruits 10g/kg; Low dose group gavage every day elaeagnus conferta fruits 2g/kg.Fasting 24h after the last administration, Cavia porcellus eye socket place's injection air is lethal, cuts the abdominal cavity open, blocks cystic duct with vascular forceps, takes off gallbladder, extracts bile out with syringe and measures calcium ion (Ca 2+) content, total bilirubin, conjugated bilirubin and calculate unconjugated bilirubin concentration, cut at last gallbladder open, calculate every composition stone number of elements and gallstone formation rate, the calculus censorship is qualitative.Calcium ion content is measured and is used the methyl thymol blue colorimetry.Total bilirubin, calculus determination of bilirubin vanadate oxidizing process.The calculus way of qualitative analysis: get a little calculus powder and put in vitro, add dehydrated alcohol 3mL, add freshly prepared diazo reagent 1mL after note, shaking up rear appearance red is that bilirubin is positive.Experimental result is used the SPSS statistical software.Measurement data with
Figure BSA00000869439000061
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the gallstone formation rate of model group, calcium ion content, total bilirubin concentration, unconjugated bilirubin concentration all are significantly higher than matched group, prompting stone model modeling success.High dose group can significantly reduce calculus rate, calcium ion content, total bilirubin concentration and unconjugated bilirubin concentration.Experimental result sees Table 3,4.
The impact of table 3 elaeagnus conferta fruits on Cavia porcellus bilirubinoid cholelithiasis
Figure BSA00000869439000062
The impact of table 4 elaeagnus conferta fruits on bilirubinoid cholelithiasis Cavia porcellus conjugated bilirubin, unconjugated bilirubin, total bilirubin
Figure BSA00000869439000063
Figure BSA00000869439000064
Annotate: compare with model group, *P＜0.05, *P＜0.01
Elaeagnus conferta fruits treatment cholesterol cholelithiasis
Experimental technique:
Get 50 of Cavia porcelluss (female, body weight 250-300g cleans level, and Chinese pharmaceutical biological product identifies that institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.The matched group Cavia porcellus is raised and uses normal diet, and all the other are respectively organized the Cavia porcellus raising and use the cholesterol cholelithiasis to cause the stone feedstuff, and filling a prescription is: Cavia porcellus feedstuff 95.45%, casein 1%, sucrose 1.5%, Adeps Sus domestica 1%, cellulose 1%, cholesterol 0.05%.It is administration that modeling begins, every day 1 time, continuous 8 weeks.Positive control group selection Chinese medicine Fangshi capsule is as positive control drug, every day gavage 3g/kg.The fragmentation of elaeagnus conferta fruits fruit is made suspension with 0.5% sodium carboxymethyl cellulose solution afterwards.High dose group gavage every day elaeagnus conferta fruits 10g/kg; Low dose group gavage every day elaeagnus conferta fruits 2g/kg.Fasting 24h after the last administration, Cavia porcellus eye socket place's injection air is lethal, cuts the abdominal cavity open, block cystic duct with vascular forceps, take off gallbladder, extract the bile censorship and measure calcium ion and cholesterol concentration, and cut gallbladder open, and calculate every composition stone number of elements and gallstone formation rate, the calculus censorship is qualitative.Cholesterol determination enzymic colorimetric, calcium are measured and are stretched the III method with azo.The calculus way of qualitative analysis: get 10mg calculus powder and put into and in vitro add chloroform 10mL, fully mix approximately filter 23 mL after 15min, after adding acetic anhydride 1mL and concentrated sulphuric acid 0.2mL and mixing, put dark place 10min, aobvious aeruginous person is the cholesterol radiopaque calculus.Experimental result is used the SPSS statistical software.Measurement data with
Figure BSA00000869439000072
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the gallstone formation rate of model group, the calcium ion in bile and cholesterol concentration are significantly higher than matched group, prompting stone model modeling success.High dose group can significantly reduce calcium ion and the cholesterol concentration in calculus rate, bile.Experimental result sees Table 5,6.
The impact of table 5 elaeagnus conferta fruits on Cavia porcellus cholesterol cholelithiasis
Figure BSA00000869439000073
Figure BSA00000869439000081
The impact of table 6 elaeagnus conferta fruits on calcium ion and cholesterol concentration in cholesterol cholelithiasis guinea pig bile
Figure BSA00000869439000082
Figure BSA00000869439000083
Annotate: compare with model group, *P＜0.05, *P＜0.01
Elaeagnus conferta fruits treatment hyperuricemia
Experimental technique:
Get 50 of SD rats (male, body weight 180-220g, the SPF level, laboratory animal resource center of Nat'l Pharmaceutical ﹠ Biological Products Control Institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.Each is organized rat feeding and all uses normal diet and distilled water.Model group, positive controls, high dose group, four groups of Cavia porcellus Intraperitoneal injection of hypoxanthine every day 1000mg/kg of low dose group.It is administration that modeling begins, every day 1 time, continuous 8 weeks.Positive control group selection Allopurinol Tablets is as positive control drug, every day gavage 3g/kg.The fragmentation of elaeagnus conferta fruits fruit is made suspension with 0.5% sodium carboxymethyl cellulose solution afterwards.High dose group gavage every day elaeagnus conferta fruits 10g/kg; Low dose group gavage every day elaeagnus conferta fruits 2g/kg.After last administration 1h, rat eye socket rear vein beard is taken a blood sample, and blood sample is placed in respectively the centrifuge tube of 1.5mL, natural blood coagulation 1h under room temperature, 2500rmin -1Centrifugal 5min gets upper serum, detects assay kit (BioVision company produce) with uric acid and measures the serum uric acid value.Experimental result is used the SPSS statistical software.Measurement data with X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the serum uric acid concentration of model group is significantly higher than matched group, prompting hyperuricemia model modeling success.High dose group can significantly reduce serum uric acid concentration.Experimental result sees Table 7.
The impact of table 7 elaeagnus conferta fruits on the guinea pig serum uric acid concentration
Annotate: compare with model group, *P＜0.05, *P＜0.01
Embodiment 2
The preparation of elaeagnus conferta fruits water extract
Elaeagnus conferta fruits is selected, cleaning, gets 2500g, smashs to pieces on bruisher, adds water 10L, decocts and extracts 30 minutes, filters, and filtrate is concentrated into 500mL, namely gets the elaeagnus conferta fruits water extract.
Elaeagnus conferta fruits water extract treatment renal calculus
Experimental technique:
Get 50 of Wistar rats (male, body weight 180-220g, the SPF level, Chinese pharmaceutical biological product identifies that institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.Each is organized rat and all uses normal diet and distilled water to raise.Model group, positive controls, high dose group, four groups of rats of low dose group are only used 1% ethylene glycol (EG)+2% ammonium chloride (AC) gavage every day 2mL/.It is administration that modeling begins, every day 1 time, continuous 8 weeks.Positive control group selection Chinese medicine granules for treating nephrolith is as positive control drug, every day gavage 3g/kg.High dose group gavage every day elaeagnus conferta fruits water extract 50g/kg (crude drug dosage); Low dose group gavage every day 10g/kg elaeagnus conferta fruits water extract (crude drug dosage).After last administration 1h, use the pentobarbital sodium anesthetized animal, abdominal aortic blood detects blood urea nitrogen, creatinine content, gets kidney and detects Kidney coefficients, gets left kidney and surveys the kidney calcium content, and right kidney is done pathological examination, surveys gallstone formation rate.Experimental result is used the SPSS statistical software.Measurement data with
Figure BSA00000869439000092
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the gallstone formation rate of model group is significantly higher than matched group, and Kidney coefficients is significantly lower than matched group, and kidney calcium content, serum urea nitrogen and serum creatinine are significantly higher than matched group, prompting stone model modeling success.High dose group can significantly reduce the calculus rate, improves Kidney coefficients, reduces kidney calcium content, serum urea nitrogen and serum creatinine.Experimental result sees Table 8,9.
The impact of table 8 elaeagnus conferta fruits water extract on the kidney of rats calculus
Figure BSA00000869439000101
The impact of table 9 elaeagnus conferta fruits water extract on large mouse with renal calculs Kidney coefficients, kidney calcium content, serum creatinine and serum urea nitrogen
Figure BSA00000869439000102
Annotate: compare with model group, *P＜0.05, *P＜0.01
Elaeagnus conferta fruits water extract treatment bilirubinoid cholelithiasis
Experimental technique:
Get 50 of Cavia porcelluss (female, body weight 250-300g cleans level, and Chinese pharmaceutical biological product identifies that institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.The matched group Cavia porcellus is raised and uses normal diet, all the other are respectively organized the Cavia porcellus raising and use the bilirubinoid cholelithiasis to cause the stone feedstuff, and filling a prescription is: normal diet 95.43%, casein 1%, sucrose 1.5%, Adeps Sus domestica 1%, cellulose 1%, cholic acid 0.02%, cholesterol 0.05%.It is administration that modeling begins, every day 1 time, continuous 8 weeks.Positive control group selection Chinese medicine Fangshi capsule is as positive control drug, every day gavage 3g/kg.High dose group gavage every day elaeagnus conferta fruits water extract 50g/kg (crude drug dosage); Low dose group gavage every day elaeagnus conferta fruits water extract 10g/kg (crude drug dosage).Fasting 24h after the last administration, Cavia porcellus eye socket place's injection air is lethal, cuts the abdominal cavity open, blocks cystic duct with vascular forceps, takes off gallbladder, extracts bile out with syringe and measures calcium ion (Ca 2+) content, total bilirubin, conjugated bilirubin and calculate unconjugated bilirubin concentration, cut at last gallbladder open, calculate every composition stone number of elements and gallstone formation rate, the calculus censorship is qualitative.Calcium ion content is measured and is used the methyl thymol blue colorimetry.Total bilirubin, calculus determination of bilirubin vanadate oxidizing process.The calculus way of qualitative analysis: get a little calculus powder and put in vitro, add dehydrated alcohol 3mL, add the actual 1mL of freshly prepared diazonium after note, shaking up rear appearance red is that bilirubin is positive.Measurement data with
Figure BSA00000869439000111
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the gallstone formation rate of model group, calcium ion content, total bilirubin concentration, unconjugated bilirubin concentration all are significantly higher than matched group, prompting stone model modeling success.High dose group can significantly reduce calculus rate, calcium ion content, total bilirubin concentration and unconjugated bilirubin concentration.Experimental result sees Table 10,11.
The impact of table 10 elaeagnus conferta fruits water extract on Cavia porcellus bilirubinoid cholelithiasis
Figure BSA00000869439000112
The impact of table 11 elaeagnus conferta fruits water extract on bilirubinoid cholelithiasis Cavia porcellus conjugated bilirubin, unconjugated bilirubin, total bilirubin
Figure BSA00000869439000113
Figure BSA00000869439000114
Annotate: compare with model group, *P＜0.05, *P＜0.01
Elaeagnus conferta fruits water extract treatment cholesterol cholelithiasis
Experimental technique:
Get 50 of Cavia porcelluss (female, body weight 250-300g cleans level, and Chinese pharmaceutical biological product identifies that institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.The matched group Cavia porcellus is raised and uses normal diet, and all the other are respectively organized the Cavia porcellus raising and use the cholesterol cholelithiasis to cause the stone feedstuff, and filling a prescription is: Cavia porcellus feedstuff 95.45%, casein 1%, sucrose 1.5%, Adeps Sus domestica 1%, cellulose 1%, cholesterol 0.05%.It is administration that modeling begins, every day 1 time, continuous 8 weeks.Positive control group selection Chinese medicine Fangshi capsule is as positive control drug, every day gavage 3g/kg.High dose group gavage every day elaeagnus conferta fruits water extract 50g/kg (crude drug dosage); Low dose group gavage every day elaeagnus conferta fruits water extract 10g/kg (crude drug dosage).Fasting 24h after the last administration, Cavia porcellus eye socket place's injection air is lethal, cuts the abdominal cavity open, block cystic duct with vascular forceps, take off gallbladder, extract the bile censorship and measure calcium ion and cholesterol concentration, and cut gallbladder open, and calculate every composition stone number of elements and gallstone formation rate, the calculus censorship is qualitative.Cholesterol determination enzymic colorimetric, calcium are measured and are stretched the III method with azo.The calculus way of qualitative analysis: get 10mg calculus powder and put into and in vitro add chloroform 10mL, fully mix approximately filter 23 mL after 15min, after adding acetic anhydride 1mL and concentrated sulphuric acid 0.2mL and mixing, put dark place 10min, aobvious aeruginous person is the cholesterol radiopaque calculus.Measurement data with
Figure BSA00000869439000122
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the gallstone formation rate of model group, the calcium ion in bile and cholesterol concentration are significantly higher than matched group, prompting stone model modeling success.High dose group can significantly reduce calcium ion and the cholesterol concentration in calculus rate, bile.Experimental result sees Table 12,13.
The impact of table 12 elaeagnus conferta fruits water extract on Cavia porcellus cholesterol cholelithiasis
Figure BSA00000869439000123
The impact of table 13 elaeagnus conferta fruits water extract on calcium ion and cholesterol concentration in cholesterol cholelithiasis guinea pig bile
Figure BSA00000869439000131
Figure BSA00000869439000132
Figure BSA00000869439000133
Annotate: compare with model group, *P＜0.05, *P＜0.01
Elaeagnus conferta fruits water extract treatment hyperuricemia
Experimental technique:
Get 50 of SD rats (male, body weight 180-220g, the SPF level, laboratory animal resource center of Nat'l Pharmaceutical ﹠ Biological Products Control Institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.Each is organized rat feeding and all uses normal diet and distilled water.Model group, positive controls, high dose group, four groups of Cavia porcellus Intraperitoneal injection of hypoxanthine every day 1000mg/kg of low dose group.It is administration that modeling begins.Fed for eight weeks continuously.Positive control group selection Allopurinol Tablets is as positive control drug, every day gavage 3g/kg.High dose group gavage every day elaeagnus conferta fruits water extract 50g/kg (crude drug dosage); Low dose group gavage every day elaeagnus conferta fruits water extract 10g/kg (crude drug dosage).After last administration 1h, rat eye socket rear vein beard is taken a blood sample, and blood sample is placed in respectively the centrifuge tube of 1.5mL, and natural blood coagulation 1h under room temperature is at 2500rmin -1Centrifugal 5min gets upper serum, detects assay kit (BioVision company produce) with uric acid and measures Level of Serum Uric Acid.Measurement data with
Figure BSA00000869439000134
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the serum uric acid concentration of model group is significantly higher than matched group, prompting hyperuricemia model modeling success.High dose group can significantly reduce serum uric acid concentration.Experimental result sees Table 14.
The impact of table 14 elaeagnus conferta fruits water extract on the guinea pig serum uric acid concentration
Figure BSA00000869439000135
Figure BSA00000869439000141
Annotate: compare with model group, *P＜0.05, *P＜0.01
Embodiment 3
The preparation of dehydration elaeagnus conferta fruits
Elaeagnus conferta fruits is selected, clean, and the acupuncture peel is used sugared salt mixed liquor permeating and dewatering 24 hours at normal temperatures and pressures, after being filtered dry water, carries out 65 ℃ of hot air convective drying 3-5 hours, ventilates to namely getting the dehydration elaeagnus conferta fruits after cooling.
Dehydration elaeagnus conferta fruits treatment renal calculus
Experimental technique:
Get 50 of Wistar rats (male, body weight 180-220g, the SPF level, Chinese pharmaceutical biological product identifies that institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.Each is organized rat and all uses normal diet and distilled water to raise.Model group, positive controls, high dose group, four groups of rats of low dose group are only used 1% ethylene glycol (EG)+2% ammonium chloride (AC) gavage every day 2mL/.It is administration that modeling begins, once a day, and continuous eight weeks.Positive control group selection Chinese medicine granules for treating nephrolith is as positive control drug, every day gavage 3g/kg.After pulverizing, the dehydration elaeagnus conferta fruits makes suspension with 0.5% sodium carboxymethyl cellulose solution.High dose group gavage every day dehydration elaeagnus conferta fruits 10g/kg; Low dose group gavage every day dehydration elaeagnus conferta fruits 2g/kg.After last administration 1h, use the pentobarbital sodium anesthetized animal, abdominal aortic blood detects blood urea nitrogen, creatinine content, gets kidney and detects Kidney coefficients, gets left kidney and surveys the kidney calcium content, and right kidney is done pathological examination, surveys gallstone formation rate.Experimental result is used the SPSS statistical software.Measurement data with
Figure BSA00000869439000142
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the gallstone formation rate of model group is significantly higher than matched group, and Kidney coefficients is significantly lower than matched group, and kidney calcium content, serum urea nitrogen and serum creatinine are significantly higher than matched group, prompting stone model modeling success.High dose group can significantly reduce the calculus rate, improves Kidney coefficients, reduces kidney calcium content, serum urea nitrogen and serum creatinine.Experimental result sees Table 15,16.
The impact of table 15 dehydration elaeagnus conferta fruits on the kidney of rats calculus
Figure BSA00000869439000151
The impact of table 16 dehydration elaeagnus conferta fruits on large mouse with renal calculs Kidney coefficients, kidney calcium content, serum creatinine and serum urea nitrogen
Figure BSA00000869439000152
Figure BSA00000869439000153
Annotate: compare with model group, *P＜0.05, *P＜0.01
Dehydration elaeagnus conferta fruits treatment bilirubinoid cholelithiasis
Experimental technique:
Get 50 of Cavia porcelluss (female, body weight 250-300g cleans level, and Chinese pharmaceutical biological product identifies that institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.The matched group Cavia porcellus is raised and uses normal diet, all the other are respectively organized the Cavia porcellus raising and use the bilirubinoid cholelithiasis to cause the stone feedstuff, and filling a prescription is: normal diet 95.43%, casein 1%, sucrose 1.5%, Adeps Sus domestica 1%, cellulose 1%, cholic acid 0.02%, cholesterol 0.05%.It is administration that modeling begins, every day 1 time, continuous 8 weeks.Positive control group selection Chinese medicine Fangshi capsule is as positive control drug, every day gavage 3g/kg.After pulverizing, the dehydration elaeagnus conferta fruits makes suspension with 0.5% sodium carboxymethyl cellulose solution.High dose group gavage every day dehydration elaeagnus conferta fruits 10g/kg; Low dose group gavage every day dehydration elaeagnus conferta fruits 2g/kg.Fasting 24h after the last administration, Cavia porcellus eye socket place's injection air is lethal, cuts the abdominal cavity open, blocks cystic duct with vascular forceps, takes off gallbladder, extracts bile out with syringe and measures calcium ion (Ca 2+) content, total bilirubin, conjugated bilirubin and calculate unconjugated bilirubin concentration, cut at last gallbladder open, calculate every composition stone number of elements and gallstone formation rate, the calculus censorship is qualitative.Calcium ion content is measured and is used the methyl thymol blue colorimetry.Total bilirubin, calculus determination of bilirubin vanadate oxidizing process.The calculus way of qualitative analysis: get a little calculus powder and put in vitro, add dehydrated alcohol 3mL, add the actual 1mL of freshly prepared diazonium after note, shaking up rear appearance red is that bilirubin is positive.Experimental result is used the SPSS statistical software.Measurement data with
Figure BSA00000869439000161
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the gallstone formation rate of model group, calcium ion content, total bilirubin concentration, unconjugated bilirubin concentration all are significantly higher than matched group, prompting stone model modeling success.High dose group can significantly reduce calculus rate, calcium ion content, total bilirubin concentration and unconjugated bilirubin concentration.Experimental result sees Table 17,18.
The impact of table 17 dehydration elaeagnus conferta fruits on Cavia porcellus bilirubinoid cholelithiasis
Figure BSA00000869439000162
The impact of table 18 dehydration elaeagnus conferta fruits on bilirubinoid cholelithiasis Cavia porcellus conjugated bilirubin, unconjugated bilirubin, total bilirubin
Figure BSA00000869439000163
Annotate: compare with model group, *P＜0.05, *P＜0.01
Dehydration elaeagnus conferta fruits treatment cholesterol cholelithiasis
Experimental technique:
Get 50 of Cavia porcelluss (female, body weight 250-300g cleans level, and Chinese pharmaceutical biological product identifies that institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.The matched group Cavia porcellus is raised and uses normal diet, and all the other are respectively organized the Cavia porcellus raising and use the cholesterol cholelithiasis to cause the stone feedstuff, and filling a prescription is: Cavia porcellus feedstuff 95.45%, casein 1%, sucrose 1.5%, Adeps Sus domestica 1%, cellulose 1%, cholesterol 0.05%.It is administration that modeling begins, every day 1 time, continuous 8 weeks.Positive control group selection Chinese medicine Fangshi capsule is as positive control drug, every day gavage 3g/kg.After pulverizing, the dehydration elaeagnus conferta fruits makes suspension with 0.5% sodium carboxymethyl cellulose solution.High dose group gavage every day dehydration elaeagnus conferta fruits 10g/kg; Low dose group gavage every day dehydration elaeagnus conferta fruits 2g/kg.Fasting 24h after the last administration, Cavia porcellus eye socket place's injection air is lethal, cuts the abdominal cavity open, block cystic duct with vascular forceps, take off gallbladder, extract the bile censorship and measure calcium ion and cholesterol concentration, and cut gallbladder open, and calculate every composition stone number of elements and gallstone formation rate, the calculus censorship is qualitative.Cholesterol determination enzymic colorimetric, calcium are measured and are stretched the III method with azo.The calculus way of qualitative analysis: get 10mg calculus powder and put into and in vitro add chloroform 10mL, fully mix approximately filter 23 mL after 15min, after adding acetic anhydride 1mL and concentrated sulphuric acid 0.2mL and mixing, put dark place 10min, aobvious aeruginous person is the cholesterol radiopaque calculus.Experimental result is used the SPSS statistical software.Measurement data with
Figure BSA00000869439000171
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the gallstone formation rate of model group, the calcium ion in bile and cholesterol concentration are significantly higher than matched group, prompting stone model modeling success.High dose group can significantly reduce calcium ion and the cholesterol concentration in calculus rate, bile.Experimental result sees Table 19,20.
The impact of table 19 dehydration elaeagnus conferta fruits on Cavia porcellus cholesterol cholelithiasis
Figure BSA00000869439000172
The impact of table 20 dehydration elaeagnus conferta fruits on calcium ion and cholesterol concentration in cholesterol cholelithiasis guinea pig bile
Figure BSA00000869439000181
Figure BSA00000869439000182
Figure BSA00000869439000183
Annotate: compare with model group, *P＜0.05, *P＜0.01
Dehydration elaeagnus conferta fruits treatment hyperuricemia
Experimental technique:
Get 50 of SD rats (male, body weight 180-220g, the SPF level, laboratory animal resource center of Nat'l Pharmaceutical ﹠ Biological Products Control Institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.Each is organized rat feeding and all uses normal diet and distilled water.Model group, positive controls, high dose group, four groups of Cavia porcellus Intraperitoneal injection of hypoxanthine every day 1000mg/kg of low dose group.It is administration that modeling begins, every day 1 time, continuous 8 weeks.Positive control group selection Allopurinol Tablets is as positive control drug, every day gavage 3g/kg.After pulverizing, the dehydration elaeagnus conferta fruits makes suspension with 0.5% sodium carboxymethyl cellulose solution.High dose group gavage every day dehydration elaeagnus conferta fruits 10g/kg; Low dose group gavage every day dehydration elaeagnus conferta fruits 2g/kg.After last administration 1h, rat eye socket rear vein beard is taken a blood sample, and blood sample is placed in respectively the centrifuge tube of 1.5mL, and natural blood coagulation 1h under room temperature is at 2500rmin -1Centrifugal 5min gets upper serum, detects assay kit (BioVision company produce) with uric acid and measures Level of Serum Uric Acid.Experimental result is used the SPSS statistical software.Measurement data with
Figure BSA00000869439000184
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the serum uric acid concentration of model group is significantly higher than matched group, prompting hyperuricemia model modeling success.High dose group can significantly reduce serum uric acid concentration.Experimental result sees Table 21.
The impact of table 21 dehydration elaeagnus conferta fruits on the guinea pig serum uric acid concentration
Figure BSA00000869439000185
Figure BSA00000869439000191
Annotate: compare with model group, *P＜0.05, *P＜0.01
Embodiment 4
The preparation of elaeagnus conferta fruits capsule
Elaeagnus conferta fruits is selected, cleaning, gets 1000g, smashs to pieces on bruisher, add 10 liters, water, decoct and extracted 2 hours, filter, filtrate is concentrated, and drying under reduced pressure is pulverized, with ethanol soft material processed, the granulation of sieving, drying, granulate, encapsulated, namely get the elaeagnus conferta fruits extract capsule, every capsules dress elaeagnus conferta fruits extract 0.3g.
Elaeagnus conferta fruits capsule for treating renal calculus
Experimental technique:
Get 50 of Wistar rats (male, body weight 180-220g, the SPF level, Chinese pharmaceutical biological product identifies that institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.Each is organized rat and all uses normal diet and distilled water to raise.Model group, positive controls, high dose group, four groups of rats of low dose group are only used 1% ethylene glycol (EG)+2% ammonium chloride (AC) gavage every day 2mL/.It is administration that modeling begins, once a day, and continuous eight weeks.Positive control group selection Chinese medicine granules for treating nephrolith is as positive control drug, every day gavage 3g/kg.In the elaeagnus conferta fruits capsule, fruit extract is made suspension with 0.5% sodium carboxymethyl cellulose solution.Fruit extract 10g/kg in high dose group gavage every day elaeagnus conferta fruits capsule; Fruit extract 2g/kg in low dose group gavage every day elaeagnus conferta fruits capsule.After last administration 1h, use the pentobarbital sodium anesthetized animal, abdominal aortic blood detects blood urea nitrogen, creatinine content, gets kidney and detects Kidney coefficients, gets left kidney and surveys the kidney calcium content, and right kidney is done pathological examination, surveys gallstone formation rate.Experimental result is used the SPSS statistical software.Measurement data with
Figure BSA00000869439000192
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the gallstone formation rate of model group is significantly higher than matched group, and Kidney coefficients is significantly lower than matched group, and kidney calcium content, serum urea nitrogen and serum creatinine are significantly higher than matched group, prompting stone model modeling success.High dose group can significantly reduce the calculus rate, improves Kidney coefficients, reduces kidney calcium content, serum urea nitrogen and serum creatinine.Experimental result sees Table 22,23.
The impact of table 22 elaeagnus conferta fruits Capsule in Rats renal calculus
Figure BSA00000869439000201
The impact of table 23 elaeagnus conferta fruits capsule on large mouse with renal calculs Kidney coefficients, kidney calcium content, serum creatinine and serum urea nitrogen
Figure BSA00000869439000203
Annotate: compare with model group, *P＜0.05, *P＜0.01
Elaeagnus conferta fruits capsule for treating bilirubinoid cholelithiasis
Experimental technique:
Get 50 of Cavia porcelluss (female, body weight 250-300g cleans level, and Chinese pharmaceutical biological product identifies that institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.The matched group Cavia porcellus is raised and uses normal diet, all the other are respectively organized the Cavia porcellus raising and use the bilirubinoid cholelithiasis to cause the stone feedstuff, and filling a prescription is: normal diet 95.43%, casein 1%, sucrose 1.5%, Adeps Sus domestica 1%, cellulose 1%, cholic acid 0.02%, cholesterol 0.05%.It is administration that modeling begins, every day 1 time, continuous 8 weeks.Positive control group selection Chinese medicine Fangshi capsule is as positive control drug, every day gavage 3g/kg.In the elaeagnus conferta fruits capsule, fruit extract is made suspension with 0.5% sodium carboxymethyl cellulose solution.Fruit extract 10g/kg in high dose group gavage every day elaeagnus conferta fruits capsule; Fruit extract 2g/kg in low dose group gavage every day elaeagnus conferta fruits capsule.Fasting 24h after the last administration, Cavia porcellus eye socket place's injection air is lethal, cuts the abdominal cavity open, blocks cystic duct with vascular forceps, takes off gallbladder, extracts bile out with syringe and measures calcium ion (Ca 2+) content, total bilirubin, conjugated bilirubin and calculate unconjugated bilirubin concentration, cut at last gallbladder open, calculate every composition stone number of elements and gallstone formation rate, the calculus censorship is qualitative.Calcium ion content is measured and is used the methyl thymol blue colorimetry.Total bilirubin, calculus determination of bilirubin vanadate oxidizing process.The calculus way of qualitative analysis: get a little calculus powder and put in vitro, add dehydrated alcohol 3mL, add the actual 1mL of freshly prepared diazonium after note, shaking up rear appearance red is that bilirubin is positive.Experimental result is used the SPSS statistical software.Measurement data with X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the gallstone formation rate of model group, calcium ion content, total bilirubin concentration, unconjugated bilirubin concentration all are significantly higher than matched group, prompting stone model modeling success.High dose group can significantly reduce calculus rate, calcium ion content, total bilirubin concentration and unconjugated bilirubin concentration.Experimental result sees Table 24,25.
The impact of table 24 elaeagnus conferta fruits capsule on Cavia porcellus bilirubinoid cholelithiasis
The impact of table 25 elaeagnus conferta fruits capsule on bilirubinoid cholelithiasis Cavia porcellus conjugated bilirubin, unconjugated bilirubin, total bilirubin
Figure BSA00000869439000214
Annotate: compare with model group, *P＜0.05, *P＜0.01
Elaeagnus conferta fruits capsule for treating cholesterol cholelithiasis
Experimental technique:
Get 50 of Cavia porcelluss (female, body weight 250-300g cleans level, and Chinese pharmaceutical biological product identifies that institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.The matched group Cavia porcellus is raised and uses normal diet, and all the other are respectively organized the Cavia porcellus raising and use the cholesterol cholelithiasis to cause the stone feedstuff, and filling a prescription is: Cavia porcellus feedstuff 95.45%, casein 1%, sucrose 1.5%, Adeps Sus domestica 1%, cellulose 1%, cholesterol 0.05%.It is administration that modeling begins, every day 1 time, continuous 8 weeks.Positive control group selection Chinese medicine Fangshi capsule is as positive control drug, every day gavage 3g/kg.In the elaeagnus conferta fruits capsule, fruit extract is made suspension with 0.5% sodium carboxymethyl cellulose solution.Fruit extract 10g/kg in high dose group gavage every day elaeagnus conferta fruits capsule; Fruit extract 2g/kg in low dose group gavage every day elaeagnus conferta fruits capsule.Fasting 24h after the last administration, Cavia porcellus eye socket place's injection air is lethal, cuts the abdominal cavity open, block cystic duct with vascular forceps, take off gallbladder, extract the bile censorship and measure calcium ion and cholesterol concentration, and cut gallbladder open, and calculate every composition stone number of elements and gallstone formation rate, the calculus censorship is qualitative.Cholesterol determination enzymic colorimetric, calcium are measured and are stretched the III method with azo.The calculus way of qualitative analysis: get 10mg calculus powder and put into and in vitro add chloroform 10mL, fully mix approximately filter 23 mL after 15min, after adding acetic anhydride 1mL and concentrated sulphuric acid 0.2mL and mixing, put dark place 10min, aobvious aeruginous person is the cholesterol radiopaque calculus.Experimental result is used the SPSS statistical software.Measurement data with
Figure BSA00000869439000221
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the gallstone formation rate of model group, the calcium ion in bile and cholesterol concentration are significantly higher than matched group, prompting stone model modeling success.High dose group can significantly reduce calcium ion and the cholesterol concentration in calculus rate, bile.Experimental result sees Table 26,27.
The impact of table 26 elaeagnus conferta fruits capsule on Cavia porcellus cholesterol cholelithiasis
Figure BSA00000869439000222
The impact of table 27 elaeagnus conferta fruits capsule on calcium ion and cholesterol concentration in cholesterol cholelithiasis guinea pig bile
Figure BSA00000869439000231
Annotate: compare with model group, *P＜0.05, *P＜0.01
Elaeagnus conferta fruits capsule for treating hyperuricemia
Experimental technique:
Get 50 of SD rats (male, body weight 180-220g, the SPF level, laboratory animal resource center of Nat'l Pharmaceutical ﹠ Biological Products Control Institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.Each is organized rat feeding and all uses normal diet and distilled water.Model group, positive controls, high dose group, four groups of Cavia porcellus Intraperitoneal injection of hypoxanthine every day 1000mg/kg of low dose group.It is administration that modeling begins, once a day, and continuous eight weeks.Positive control group selection Allopurinol Tablets is as positive control drug, every day gavage 3g/kg.In the elaeagnus conferta fruits capsule, fruit extract is made suspension with 0.5% sodium carboxymethyl cellulose solution.Fruit extract 10g/kg in high dose group gavage every day elaeagnus conferta fruits capsule; Fruit extract 2g/kg in low dose group gavage every day elaeagnus conferta fruits capsule.After last administration 1h, rat eye socket rear vein beard is taken a blood sample, and blood sample is placed in respectively the centrifuge tube of 1.5mL, and natural blood coagulation 1h under room temperature is at 2500rmin -1Centrifugal 5min gets upper serum, detects assay kit (BioVision company produce) with uric acid and measures Level of Serum Uric Acid.Experimental result is used the SPSS statistical software.Measurement data with
Figure BSA00000869439000234
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the serum uric acid concentration of model group is significantly higher than matched group, prompting hyperuricemia model modeling success.High dose group can significantly reduce serum uric acid concentration.Experimental result sees Table 28.
The impact of table 28 dehydration elaeagnus conferta fruits on the guinea pig serum uric acid concentration
Figure BSA00000869439000241
Annotate: compare with model group, *P＜0.05, *P＜0.01
Embodiment 5
The preparation of elaeagnus conferta fruits tablet
Elaeagnus conferta fruits is selected, cleaning, gets 1000g, smashs to pieces on bruisher, add 10 liters, water, decoct and extracted 2 hours, filter, filtrate is concentrated, and drying under reduced pressure is pulverized, add adjuvant mannitol, aspartame, magnesium stearate (elaeagnus conferta fruits powder: mannitol: aspartame: magnesium stearate=3: 2: 0.05: 0.1) after mix homogeneously, with ethanol soft material processed, the granulation of sieving, drying, granulate, tabletting, namely get the elaeagnus conferta fruits tablet, every contains elaeagnus conferta fruits extract 0.3g.
Elaeagnus conferta fruits tablet in treatment renal calculus
Experimental technique:
Get 50 of Wistar rats (male, body weight 180-220g, the SPF level, Chinese pharmaceutical biological product identifies that institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.Each is organized rat and all uses normal diet and distilled water to raise.Model group, positive controls, high dose group, four groups of rats of low dose group are only used 1% ethylene glycol (EG)+2% ammonium chloride (AC) gavage every day 2mL/.It is administration that modeling begins, once a day, and continuous eight weeks.Positive control group selection Chinese medicine granules for treating nephrolith is as positive control drug, every day gavage 3g/kg.The elaeagnus conferta fruits tablet is made suspension with 0.5% sodium carboxymethyl cellulose solution after grinding.High dose group gavage every day elaeagnus conferta fruits tablet 10g/kg; Low dose group gavage every day elaeagnus conferta fruits tablet 2g/kg.After last administration 1h, use the pentobarbital sodium anesthetized animal, abdominal aortic blood detects blood urea nitrogen, creatinine content, gets kidney and detects Kidney coefficients, gets left kidney and surveys the kidney calcium content, and right kidney is done pathological examination, surveys gallstone formation rate.Experimental result is used the SPSS statistical software.Measurement data with
Figure BSA00000869439000242
Figure BSA00000869439000243
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the gallstone formation rate of model group is significantly higher than matched group, and Kidney coefficients is significantly lower than matched group, and kidney calcium content, serum urea nitrogen and serum creatinine are significantly higher than matched group, prompting stone model modeling success.High dose group can significantly reduce the calculus rate, improves Kidney coefficients, reduces kidney calcium content, serum urea nitrogen and serum creatinine.Experimental result sees Table 29,30.
The impact of table 29 elaeagnus conferta fruits tablet on the kidney of rats calculus
Figure BSA00000869439000251
The impact of table 30 elaeagnus conferta fruits tablet on large mouse with renal calculs Kidney coefficients, kidney calcium content, serum creatinine and serum urea nitrogen
Figure BSA00000869439000252
Figure BSA00000869439000253
Annotate: compare with model group, *P＜0.05, *P＜0.01
Elaeagnus conferta fruits tablet in treatment bilirubinoid cholelithiasis
Experimental technique:
Get 50 of Cavia porcelluss (female, body weight 250-300g cleans level, and Chinese pharmaceutical biological product identifies that institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.The matched group Cavia porcellus is raised and uses normal diet, all the other are respectively organized the Cavia porcellus raising and use the bilirubinoid cholelithiasis to cause the stone feedstuff, and filling a prescription is: normal diet 95.43%, casein 1%, sucrose 1.5%, Adeps Sus domestica 1%, cellulose 1%, cholic acid 0.02%, cholesterol 0.05%.It is administration that modeling begins, every day 1 time, continuous 8 weeks.Positive control group selection Chinese medicine Fangshi capsule is as positive control drug, every day gavage 3g/kg.The elaeagnus conferta fruits tablet is made suspension with 0.5% sodium carboxymethyl cellulose solution after grinding.High dose group gavage every day elaeagnus conferta fruits tablet 10g/kg; Low dose group gavage every day elaeagnus conferta fruits tablet 2g/kg.Fasting 24h after the last administration, Cavia porcellus eye socket place's injection air is lethal, cuts the abdominal cavity open, blocks cystic duct with vascular forceps, takes off gallbladder, extracts bile out with syringe and measures calcium ion (Ca 2+) content, total bilirubin, conjugated bilirubin and calculate unconjugated bilirubin concentration, cut at last gallbladder open, calculate every composition stone number of elements and gallstone formation rate, the calculus censorship is qualitative.Calcium ion content is measured and is used the methyl thymol blue colorimetry.Total bilirubin, calculus determination of bilirubin vanadate oxidizing process.The calculus way of qualitative analysis: get a little calculus powder and put in vitro, add dehydrated alcohol 3mL, add the actual 1mL of freshly prepared diazonium after note, shaking up rear appearance red is that bilirubin is positive.Experimental result is used the SPSS statistical software.Measurement data with
Figure BSA00000869439000261
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the gallstone formation rate of model group, calcium ion content, total bilirubin concentration, unconjugated bilirubin concentration all are significantly higher than matched group, prompting stone model modeling success.High dose group can significantly reduce calculus rate, calcium ion content, total bilirubin concentration and unconjugated bilirubin concentration.Experimental result sees Table 31,32.
The impact of table 31 elaeagnus conferta fruits tablet on Cavia porcellus bilirubinoid cholelithiasis
Figure BSA00000869439000262
The impact of table 32 elaeagnus conferta fruits tablet on bilirubinoid cholelithiasis Cavia porcellus conjugated bilirubin, unconjugated bilirubin, total bilirubin
Figure BSA00000869439000271
Annotate: compare with model group, *P＜0.05, *P＜0.01
Elaeagnus conferta fruits tablet in treatment cholesterol cholelithiasis
Experimental technique:
Get 50 of Cavia porcelluss (female, body weight 250-300g cleans level, and Chinese pharmaceutical biological product identifies that institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.The matched group Cavia porcellus is raised and uses normal diet, and all the other are respectively organized the Cavia porcellus raising and use the cholesterol cholelithiasis to cause the stone feedstuff, and filling a prescription is: Cavia porcellus feedstuff 95.45%, casein 1%, sucrose 1.5%, Adeps Sus domestica 1%, cellulose 1%, cholesterol 0.05%.It is administration that modeling begins, every day 1 time, continuous 8 weeks.Positive control group selection Chinese medicine Fangshi capsule is as positive control drug, every day gavage 3g/kg.The elaeagnus conferta fruits tablet is made suspension with 0.5% sodium carboxymethyl cellulose solution after grinding.High dose group gavage every day elaeagnus conferta fruits tablet 10g/kg; Low dose group gavage every day elaeagnus conferta fruits tablet 2g/kg.Fasting 24h after the last administration, Cavia porcellus eye socket place's injection air is lethal, cuts the abdominal cavity open, block cystic duct with vascular forceps, take off gallbladder, extract the bile censorship and measure calcium ion and cholesterol concentration, and cut gallbladder open, and calculate every composition stone number of elements and gallstone formation rate, the calculus censorship is qualitative.Cholesterol determination enzymic colorimetric, calcium are measured and are stretched the III method with azo.The calculus way of qualitative analysis: get 10mg calculus powder and put into and in vitro add chloroform 10mL, fully mix approximately filter 23 mL after 15min, after adding acetic anhydride 1mL and concentrated sulphuric acid 0.2mL and mixing, put dark place 10min, aobvious aeruginous person is the cholesterol radiopaque calculus.Experimental result is used the SPSS statistical software.Measurement data with
Figure BSA00000869439000272
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the gallstone formation rate of model group, the calcium ion in bile and cholesterol concentration are significantly higher than matched group, prompting stone model modeling success.High dose group can significantly reduce calcium ion and the cholesterol concentration in calculus rate, bile.Experimental result sees Table 33,34.
The impact of table 33 elaeagnus conferta fruits tablet on Cavia porcellus cholesterol cholelithiasis
Figure BSA00000869439000273
The impact of table 34 elaeagnus conferta fruits tablet on calcium ion and cholesterol concentration in cholesterol cholelithiasis guinea pig bile
Figure BSA00000869439000282
Figure BSA00000869439000284
Annotate: compare with model group, *P＜0.05, *P＜0.01
Elaeagnus conferta fruits tablet in treatment hyperuricemia
Experimental technique:
Get 50 of SD rats (male, body weight 180-220g, the SPF level, laboratory animal resource center of Nat'l Pharmaceutical ﹠ Biological Products Control Institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.Each is organized rat feeding and all uses normal diet and distilled water.Model group, positive controls, high dose group, four groups of Cavia porcellus Intraperitoneal injection of hypoxanthine every day 1000mg/kg of low dose group.It is administration that modeling begins, once a day, and continuous eight weeks.Positive control group selection Allopurinol Tablets is as positive control drug, every day gavage 3g/kg.The elaeagnus conferta fruits tablet is made suspension with 0.5% sodium carboxymethyl cellulose solution after grinding.High dose group gavage every day elaeagnus conferta fruits tablet 10g/kg; Low dose group gavage every day elaeagnus conferta fruits tablet 2g/kg.After last administration 1h, rat eye socket rear vein beard is taken a blood sample, and blood sample is placed in respectively the centrifuge tube of 1.5mL, and natural blood coagulation 1h under room temperature is at 2500rmin -1Centrifugal 5min gets upper serum, detects assay kit (BioVision company produce) with uric acid and measures Level of Serum Uric Acid.Experimental result is used the SPSS statistical software.Measurement data with
Figure BSA00000869439000285
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the serum uric acid concentration of model group is significantly higher than matched group, prompting hyperuricemia model modeling success.High dose group can significantly reduce serum uric acid concentration.Experimental result sees Table 35.
The impact of table 35 elaeagnus conferta fruits tablet on the guinea pig serum uric acid concentration
Figure BSA00000869439000291
Annotate: compare with model group, *P＜0.05, *P＜0.01
Embodiment 6
The preparation of elaeagnus conferta fruits granule
Elaeagnus conferta fruits is selected, cleaning, get 1000g, smash to pieces on bruisher, add 10 liters, water, decoct and extracted 2 hours, filter, filtrate is condensed into extractum, pulverizes after the extractum vacuum drying, with extract powder and starch, sucrose (elaeagnus conferta fruits: starch: sucrose=9: 1: 1) abundant mixing, add entry soft material processed, then adopt the granulation of boiling granulating method, granulate, classification, packing.
Elaeagnus conferta fruits Granules in Treating renal calculus
Experimental technique:
Get 50 of Wistar rats (male, body weight 180-220g, the SPF level, Chinese pharmaceutical biological product identifies that institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.Each is organized rat and all uses normal diet and distilled water to raise.Model group, positive controls, high dose group, four groups of rats of low dose group are only used 1% ethylene glycol (EG)+2% ammonium chloride (AC) gavage every day 2mL/.It is administration that modeling begins, once a day, and continuous eight weeks.Positive control group selection Chinese medicine granules for treating nephrolith is as positive control drug, every day gavage 3g/kg.0.5g adding water 50mL, the elaeagnus conferta fruits granule makes aqueous solution.High dose group gavage every day elaeagnus conferta fruits granule 10g/kg; Low dose group gavage every day elaeagnus conferta fruits granule 2g/kg.After last administration 1h, use the pentobarbital sodium anesthetized animal, abdominal aortic blood detects blood urea nitrogen, creatinine content, gets kidney and detects Kidney coefficients, gets left kidney and surveys the kidney calcium content, and right kidney is done pathological examination, surveys gallstone formation rate.Experimental result is used the SPSS statistical software.Measurement data with
Figure BSA00000869439000292
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the gallstone formation rate of model group is significantly higher than matched group, and Kidney coefficients is significantly lower than matched group, and kidney calcium content, serum urea nitrogen and serum creatinine are significantly higher than matched group, prompting stone model modeling success.High dose group can significantly reduce the calculus rate, improves Kidney coefficients, reduces kidney calcium content, serum urea nitrogen and serum creatinine.Experimental result sees Table 36,37.
The impact of table 36 elaeagnus conferta fruits granules of rats renal calculus
The impact of table 37 elaeagnus conferta fruits granule on large mouse with renal calculs Kidney coefficients, kidney calcium content, serum creatinine and serum urea nitrogen
Figure BSA00000869439000302
Figure BSA00000869439000303
Annotate: compare with model group, *P＜0.05, *P＜0.01
Elaeagnus conferta fruits Granules in Treating bilirubinoid cholelithiasis
Experimental technique:
Get 50 of Cavia porcelluss (female, body weight 250-300g cleans level, and Chinese pharmaceutical biological product identifies that institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.The matched group Cavia porcellus is raised and uses normal diet, all the other are respectively organized the Cavia porcellus raising and use the bilirubinoid cholelithiasis to cause the stone feedstuff, and filling a prescription is: normal diet 95.43%, casein 1%, sucrose 1.5%, Adeps Sus domestica 1%, cellulose 1%, cholic acid 0.02%, cholesterol 0.05%.It is administration that modeling begins, every day 1 time, continuous 8 weeks.Positive control group selection Chinese medicine Fangshi capsule is as positive control drug, every day gavage 3g/kg.0.5g adding water 50mL, the elaeagnus conferta fruits granule makes aqueous solution.High dose group gavage every day elaeagnus conferta fruits granule 10g/kg; Low dose group gavage every day elaeagnus conferta fruits granule 2g/kg.Fasting 24h after the last administration, Cavia porcellus eye socket place's injection air is lethal, cuts the abdominal cavity open, blocks cystic duct with vascular forceps, takes off gallbladder, extracts bile out with syringe and measures calcium ion (Ca 2+) content, total bilirubin, conjugated bilirubin and calculate unconjugated bilirubin concentration, cut at last gallbladder open, calculate every composition stone number of elements and gallstone formation rate, the calculus censorship is qualitative.Calcium ion content is measured and is used the methyl thymol blue colorimetry.Total bilirubin, calculus determination of bilirubin vanadate oxidizing process.The calculus way of qualitative analysis: get a little calculus powder and put in vitro, add dehydrated alcohol 3mL, add the actual 1mL of freshly prepared diazonium after note, shaking up rear appearance red is that bilirubin is positive.Experimental result is used the SPSS statistical software.Measurement data with
Figure BSA00000869439000311
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the gallstone formation rate of model group, calcium ion content, total bilirubin concentration, unconjugated bilirubin concentration all are significantly higher than matched group, prompting stone model modeling success.High dose group can significantly reduce calculus rate, calcium ion content, total bilirubin concentration and unconjugated bilirubin concentration.Experimental result sees Table 38,39.
The impact of table 38 elaeagnus conferta fruits granule on Cavia porcellus bilirubinoid cholelithiasis
Figure BSA00000869439000312
The impact of table 39 elaeagnus conferta fruits granule on bilirubinoid cholelithiasis Cavia porcellus conjugated bilirubin, unconjugated bilirubin, total bilirubin
Figure BSA00000869439000313
Figure BSA00000869439000314
Figure BSA00000869439000321
Annotate: compare with model group, *P＜0.05, *P＜0.01
Elaeagnus conferta fruits Granules in Treating cholesterol cholelithiasis
Experimental technique:
Get 50 of Cavia porcelluss (female, body weight 250-300g cleans level, and Chinese pharmaceutical biological product identifies that institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.The matched group Cavia porcellus is raised and uses normal diet, and all the other are respectively organized the Cavia porcellus raising and use the cholesterol cholelithiasis to cause the stone feedstuff, and filling a prescription is: Cavia porcellus feedstuff 95.45%, casein 1%, sucrose 1.5%, Adeps Sus domestica 1%, cellulose 1%, cholesterol 0.05%.It is administration that modeling begins, every day 1 time, continuous 8 weeks.Positive control group selection Chinese medicine Fangshi capsule is as positive control drug, every day gavage 3g/kg.0.5g adding water 50mL, the elaeagnus conferta fruits granule makes aqueous solution.High dose group gavage every day elaeagnus conferta fruits granule 10g/kg; Low dose group gavage every day elaeagnus conferta fruits granule 2g/kg.Fasting 24h after the last administration, Cavia porcellus eye socket place's injection air is lethal, cuts the abdominal cavity open, block cystic duct with vascular forceps, take off gallbladder, extract the bile censorship and measure calcium ion and cholesterol concentration, and cut gallbladder open, and calculate every composition stone number of elements and gallstone formation rate, the calculus censorship is qualitative.Cholesterol determination enzymic colorimetric, calcium are measured and are stretched the III method with azo.The calculus way of qualitative analysis: get 10mg calculus powder and put into and in vitro add chloroform 10mL, fully mix approximately filter 23 mL after 15min, after adding acetic anhydride 1mL and concentrated sulphuric acid 0.2mL and mixing, put dark place 10min, aobvious aeruginous person is the cholesterol radiopaque calculus.Experimental result is used the SPSS statistical software.Measurement data with
Figure BSA00000869439000322
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the gallstone formation rate of model group, the calcium ion in bile and cholesterol concentration are significantly higher than matched group, prompting stone model modeling success.High dose group can significantly reduce calcium ion and the cholesterol concentration in calculus rate, bile.Experimental result sees Table 40,41.
The impact of table 40 elaeagnus conferta fruits granule on Cavia porcellus cholesterol cholelithiasis
Figure BSA00000869439000323
Figure BSA00000869439000331
The impact of table 41 elaeagnus conferta fruits granule on calcium ion and cholesterol concentration in cholesterol cholelithiasis guinea pig bile
Figure BSA00000869439000332
Figure BSA00000869439000333
Figure BSA00000869439000334
Annotate: compare with model group, *P＜0.05, *P＜0.01
Elaeagnus conferta fruits Granules in Treating hyperuricemia
Experimental technique:
Get 50 of SD rats (male, body weight 180-220g, the SPF level, laboratory animal resource center of Nat'l Pharmaceutical ﹠ Biological Products Control Institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.Each is organized rat feeding and all uses normal diet and distilled water.Model group, positive controls, high dose group, four groups of Cavia porcellus Intraperitoneal injection of hypoxanthine every day 1000mg/kg of low dose group.It is administration that modeling begins.Fed for eight weeks continuously.Positive control group selection Allopurinol Tablets is as positive control drug, every day gavage 3g/kg.0.5g adding water 50mL, the elaeagnus conferta fruits granule makes aqueous solution.High dose group gavage every day elaeagnus conferta fruits granule 10g/kg; Low dose group gavage every day elaeagnus conferta fruits granule 2g/kg.After last administration 1h, rat eye socket rear vein beard is taken a blood sample, and blood sample is placed in respectively the centrifuge tube of 1.5mL, and natural blood coagulation 1h under room temperature is at 2500rmin -1Centrifugal 5min gets upper serum, detects assay kit (BioVision company produce) with uric acid and measures Level of Serum Uric Acid.Experimental result is used the SPSS statistical software.Measurement data with
Figure BSA00000869439000335
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the serum uric acid concentration of model group is significantly higher than matched group, prompting hyperuricemia model modeling success.High dose group can significantly reduce serum uric acid concentration.Experimental result sees Table 42.
The impact of table 42 elaeagnus conferta fruits granule on the guinea pig serum uric acid concentration
Figure BSA00000869439000341
Annotate: compare with model group, *P＜0.05, *P＜0.01
Embodiment 7
The preparation of elaeagnus conferta fruits fruit vinegar
Elaeagnus conferta fruits is selected, cleaning, get 1000g, smash homogenate to pieces, add 5L water in the homogenate process, the centrifugal supernatant that obtains, add 6 degree Chinese liquor (the 56 common strong, colourless liquor distilled from sorghum of degree that Beijing Red Star joint-stock company produces of converting, add the distilled water configuration to form) 30L, acetic acid bacteria bacterium liquid 2.5L, Mel 2L, by after above proportioning mixing and stirring, made fruit vinegar in 30 days in 30 ℃ of fermentations.
Elaeagnus conferta fruits fruit vinegar treatment renal calculus
Experimental technique:
Get 50 of Wistar rats (male, body weight 180-220g, the SPF level, Chinese pharmaceutical biological product identifies that institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.Each is organized rat and all uses normal diet and distilled water to raise.Model group, positive controls, high dose group, four groups of rats of low dose group are only used 1% ethylene glycol (EG)+2% ammonium chloride (AC) gavage every day 2mL/.It is administration that modeling begins, once a day, and continuous eight weeks.Positive control group selection Chinese medicine granules for treating nephrolith is as positive control drug, every day gavage 3g/kg.High dose group gavage every day elaeagnus conferta fruits fruit vinegar 20g/kg (crude drug dosage); Low dose group gavage every day elaeagnus conferta fruits fruit vinegar 4g/kg (crude drug dosage).After last administration 1h, use the pentobarbital sodium anesthetized animal, abdominal aortic blood detects blood urea nitrogen, creatinine content, gets kidney and detects Kidney coefficients, gets left kidney and surveys the kidney calcium content, and right kidney is done pathological examination, surveys gallstone formation rate.Experimental result is used the SPSS statistical software.Measurement data with
Figure BSA00000869439000342
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the gallstone formation rate of model group is significantly higher than matched group, and Kidney coefficients is significantly lower than matched group, and kidney calcium content, serum urea nitrogen and serum creatinine are significantly higher than matched group, prompting stone model modeling success.High dose group can significantly reduce the calculus rate, improves Kidney coefficients, reduces kidney calcium content, serum urea nitrogen and serum creatinine.Experimental result sees Table 43,44.
The impact of table 43 elaeagnus conferta fruits fruit vinegar on the kidney of rats calculus
The impact of table 44 elaeagnus conferta fruits fruit vinegar on large mouse with renal calculs Kidney coefficients, kidney calcium content, serum creatinine and serum urea nitrogen
Figure BSA00000869439000353
Annotate: compare with model group, *P＜0.05, *P＜0.01
Elaeagnus conferta fruits fruit vinegar treatment bilirubinoid cholelithiasis
Experimental technique:
Get 50 of Cavia porcelluss (female, body weight 250-300g cleans level, and Chinese pharmaceutical biological product identifies that institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.The matched group Cavia porcellus is raised and uses normal diet, all the other are respectively organized the Cavia porcellus raising and use the bilirubinoid cholelithiasis to cause the stone feedstuff, and filling a prescription is: normal diet 95.43%, casein 1%, sucrose 1.5%, Adeps Sus domestica 1%, cellulose 1%, cholic acid 0.02%, cholesterol 0.05%.It is administration that modeling begins, every day 1 time, continuous 8 weeks.Positive control group selection Chinese medicine Fangshi capsule is as positive control drug, every day gavage 3g/kg.High dose group gavage every day elaeagnus conferta fruits fruit vinegar 20/kg (crude drug dosage); Low dose group gavage every day elaeagnus conferta fruits fruit vinegar 4g/kg (crude drug dosage).Fasting 24h after the last administration, Cavia porcellus eye socket place's injection air is lethal, cuts the abdominal cavity open, blocks cystic duct with vascular forceps, takes off gallbladder, extracts bile out with syringe and measures calcium ion (Ca 2+) content, total bilirubin, conjugated bilirubin and calculate unconjugated bilirubin concentration, cut at last gallbladder open, calculate every composition stone number of elements and gallstone formation rate, the calculus censorship is qualitative.Calcium ion content is measured and is used the methyl thymol blue colorimetry.Total bilirubin, calculus determination of bilirubin vanadate oxidizing process.The calculus way of qualitative analysis: get a little calculus powder and put in vitro, add dehydrated alcohol 3mL, add the actual 1mL of freshly prepared diazonium after note, shaking up rear appearance red is that bilirubin is positive.Experimental result is used the SPSS statistical software.Measurement data with
Figure BSA00000869439000361
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the gallstone formation rate of model group, calcium ion content, total bilirubin concentration, unconjugated bilirubin concentration all are significantly higher than matched group, prompting stone model modeling success.High dose group can significantly reduce calculus rate, calcium ion content, total bilirubin concentration and unconjugated bilirubin concentration.Experimental result sees Table 45,46.
The impact of table 45 elaeagnus conferta fruits fruit vinegar on Cavia porcellus bilirubinoid cholelithiasis
The impact of table 46 elaeagnus conferta fruits fruit vinegar on bilirubinoid cholelithiasis Cavia porcellus conjugated bilirubin, unconjugated bilirubin, total bilirubin
Figure BSA00000869439000364
Annotate: compare with model group, *P＜0.05, *P＜0.01
Elaeagnus conferta fruits fruit vinegar treatment cholesterol cholelithiasis
Experimental technique:
Get 50 of Cavia porcelluss (female, body weight 250-300g cleans level, and Chinese pharmaceutical biological product identifies that institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.The matched group Cavia porcellus is raised and uses normal diet, and all the other are respectively organized the Cavia porcellus raising and use the cholesterol cholelithiasis to cause the stone feedstuff, and filling a prescription is: Cavia porcellus feedstuff 95.45%, casein 1%, sucrose 1.5%, Adeps Sus domestica 1%, cellulose 1%, cholesterol 0.05%.It is administration that modeling begins, and each group gives the equal-volume distilled water, every day 1 time, continuous 8 weeks.Positive control group selection Chinese medicine Fangshi capsule is as positive control drug, every day gavage 3g/kg.High dose group gavage every day elaeagnus conferta fruits fruit vinegar 20g/kg (crude drug dosage); Low dose group gavage every day elaeagnus conferta fruits fruit vinegar 4g/kg (crude drug dosage).Fasting 24h after the last administration, Cavia porcellus eye socket place's injection air is lethal, cuts the abdominal cavity open, block cystic duct with vascular forceps, take off gallbladder, extract the bile censorship and measure calcium ion and cholesterol concentration, and cut gallbladder open, and calculate every composition stone number of elements and gallstone formation rate, the calculus censorship is qualitative.Cholesterol determination enzymic colorimetric, calcium are measured and are stretched the III method with azo.The calculus way of qualitative analysis: get 10mg calculus powder and put into and in vitro add chloroform 10mL, fully mix approximately filter 23 mL after 15min, after adding acetic anhydride 1mL and concentrated sulphuric acid 0.2mL and mixing, put dark place 10min, aobvious aeruginous person is the cholesterol radiopaque calculus.Experimental result is used the SPSS statistical software.Measurement data with
Figure BSA00000869439000372
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the gallstone formation rate of model group, the calcium ion in bile and cholesterol concentration are significantly higher than matched group, prompting stone model modeling success.High dose group can significantly reduce calcium ion and the cholesterol concentration in calculus rate, bile.Experimental result sees Table 47,48.
The impact of table 47 elaeagnus conferta fruits fruit vinegar on Cavia porcellus cholesterol cholelithiasis
Figure BSA00000869439000373
Figure BSA00000869439000381
The impact of table 48 elaeagnus conferta fruits fruit vinegar on calcium ion and cholesterol concentration in cholesterol cholelithiasis guinea pig bile
Figure BSA00000869439000382
Figure BSA00000869439000383
Figure BSA00000869439000384
Annotate: compare with model group, *P＜0.05, *P＜0.01
Elaeagnus conferta fruits fruit vinegar treatment hyperuricemia
Experimental technique:
Get 50 of SD rats (male, body weight 180-220g, the SPF level, laboratory animal resource center of Nat'l Pharmaceutical ﹠ Biological Products Control Institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.Each is organized rat feeding and all uses normal diet and distilled water.Model group, positive controls, high dose group, four groups of Cavia porcellus Intraperitoneal injection of hypoxanthine every day 1000mg/kg of low dose group.It is administration that modeling begins, once a day, and continuous eight weeks.Positive control group selection Allopurinol Tablets is as positive control drug, every day gavage 3g/kg.High dose group gavage every day elaeagnus conferta fruits fruit vinegar 20g/kg (crude drug dosage); Low dose group gavage every day elaeagnus conferta fruits fruit vinegar 4g/kg (crude drug dosage).After last administration 1h, rat eye socket rear vein beard is taken a blood sample, and blood sample is placed in respectively the centrifuge tube of 1.5mL, and natural blood coagulation 1h under room temperature is at 2500rmin -1Centrifugal 5min gets upper serum, detects assay kit (BioVision company produce) with uric acid and measures Level of Serum Uric Acid.Experimental result is used the SPSS statistical software.Measurement data with X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the serum uric acid concentration of model group is significantly higher than matched group, prompting hyperuricemia model modeling success.High dose group can significantly reduce serum uric acid concentration.Experimental result sees Table 49.
The impact of table 49 elaeagnus conferta fruits fruit vinegar on the guinea pig serum uric acid concentration
Figure BSA00000869439000391
Annotate: compare with model group, *P＜0.05, *P＜0.01
Embodiment 8
The preparation of elaeagnus conferta fruits fruit jam
Elaeagnus conferta fruits is selected, cleaning, gets 1000g, is heated to rapidly 100 ℃ and keeps 15min, makes pulp softening, and the peeling enucleation adds distilled water 500mL, uses the beater making beating, uses colloid mills further to make elaeagnus conferta fruits particle sice in serosity uniformity.The elaeagnus conferta fruits serosity that obtains is heated to 80 ℃ concentrates, in concentration process, one side rapid stirring adds Sucrose for five times on one side, adds the sucrose gross weight to account for 45% of concentrated front elaeagnus conferta fruits serosity.When being concentrated into solid content and being 60%, add compound adhesive (agar: xanthan gum: carrageenan: guar gum: distilled water=0.3: 0.3: 0.2: 0.3: 99, need through abundant imbibition after allotment), stir.Tinning, packing and seal process are no more than 30min.Pasteurize 15min namely gets elaeagnus conferta fruits fruit jam.
Elaeagnus conferta fruits fruit jam treatment renal calculus
Experimental technique:
Get 50 of Wistar rats (male, body weight 180-220g, the SPF level, Chinese pharmaceutical biological product identifies that institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.Each is organized rat and all uses normal diet and distilled water to raise.Model group, positive controls, high dose group, four groups of rats of low dose group are only used 1% ethylene glycol (EG)+2% ammonium chloride (AC) gavage every day 2mL/.It is administration that modeling begins, once a day, and continuous eight weeks.Positive control group selection Chinese medicine granules for treating nephrolith is as positive control drug, every day gavage 3g/kg.Elaeagnus conferta fruits fruit jam is made suspension with 0.5% sodium carboxymethyl cellulose solution.High dose group gavage every day elaeagnus conferta fruits fruit jam suspension 35g/kg (crude drug dosage); Low dose group gavage every day elaeagnus conferta fruits fruit jam suspension 7g/kg (crude drug dosage).After last administration 1h, use the pentobarbital sodium anesthetized animal, abdominal aortic blood detects blood urea nitrogen, creatinine content, gets kidney and detects Kidney coefficients, gets left kidney and surveys the kidney calcium content, and right kidney is done pathological examination, surveys gallstone formation rate.Experimental result is used the SPSS statistical software.Measurement data with
Figure BSA00000869439000401
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the gallstone formation rate of model group is significantly higher than matched group, and Kidney coefficients is significantly lower than matched group, and kidney calcium content, serum urea nitrogen and serum creatinine are significantly higher than matched group, prompting stone model modeling success.High dose group can significantly reduce the calculus rate, improves Kidney coefficients, reduces kidney calcium content, serum urea nitrogen and serum creatinine.Experimental result sees Table 50,51.
The impact of table 50 elaeagnus conferta fruits fruit jam on the kidney of rats calculus
Figure BSA00000869439000402
The impact of table 51 elaeagnus conferta fruits fruit jam on large mouse with renal calculs Kidney coefficients, kidney calcium content, serum creatinine and serum urea nitrogen
Figure BSA00000869439000404
Annotate: compare with model group, *P＜0.05, *P＜0.01
Elaeagnus conferta fruits fruit jam treatment bilirubinoid cholelithiasis
Experimental technique:
Get 50 of Cavia porcelluss (female, body weight 250-300g cleans level, and Chinese pharmaceutical biological product identifies that institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.The matched group Cavia porcellus is raised and uses normal diet, all the other are respectively organized the Cavia porcellus raising and use the bilirubinoid cholelithiasis to cause the stone feedstuff, and filling a prescription is: normal diet 95.43%, casein 1%, sucrose 1.5%, Adeps Sus domestica 1%, cellulose 1%, cholic acid 0.02%, cholesterol 0.05%.It is administration that modeling begins, every day 1 time, continuous 8 weeks.Positive control group selection Chinese medicine Fangshi capsule is as positive control drug, every day gavage 3g/kg.Elaeagnus conferta fruits fruit jam is made suspension with 0.5% sodium carboxymethyl cellulose solution.High dose group gavage every day elaeagnus conferta fruits fruit jam suspension 35g/kg (crude drug dosage); Low dose group gavage every day elaeagnus conferta fruits fruit jam suspension 7g/kg (crude drug dosage).Fasting 24h after the last administration, Cavia porcellus eye socket place's injection air is lethal, cuts the abdominal cavity open, blocks cystic duct with vascular forceps, takes off gallbladder, extracts bile out with syringe and measures calcium ion (Ca 2+) content, total bilirubin, conjugated bilirubin and calculate unconjugated bilirubin concentration, cut at last gallbladder open, calculate every composition stone number of elements and gallstone formation rate, the calculus censorship is qualitative.Calcium ion content is measured and is used the methyl thymol blue colorimetry.Total bilirubin, calculus determination of bilirubin vanadate oxidizing process.The calculus way of qualitative analysis: get a little calculus powder and put in vitro, add dehydrated alcohol 3mL, add freshly prepared diazo reagent 1mL after note, shaking up rear appearance red is that bilirubin is positive.Experimental result is used the SPSS statistical software.Measurement data with
Figure BSA00000869439000411
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the gallstone formation rate of model group, calcium ion content, total bilirubin concentration, unconjugated bilirubin concentration all are significantly higher than matched group, prompting stone model modeling success.High dose group can significantly reduce calculus rate, calcium ion content, total bilirubin concentration and unconjugated bilirubin concentration.Experimental result sees Table 52,53.
The impact of table 52 elaeagnus conferta fruits fruit jam on Cavia porcellus bilirubinoid cholelithiasis
Figure BSA00000869439000412
The impact of table 53 elaeagnus conferta fruits fruit jam on bilirubinoid cholelithiasis Cavia porcellus conjugated bilirubin, unconjugated bilirubin, total bilirubin
Figure BSA00000869439000413
Figure BSA00000869439000414
Figure BSA00000869439000421
Annotate: compare with model group, *P＜0.05, *P＜0.01
Elaeagnus conferta fruits fruit jam treatment cholesterol cholelithiasis
Experimental technique:
Get 50 of Cavia porcelluss (female, body weight 250-300g cleans level, and Chinese pharmaceutical biological product identifies that institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.The matched group Cavia porcellus is raised and uses normal diet, and all the other are respectively organized the Cavia porcellus raising and use the cholesterol cholelithiasis to cause the stone feedstuff, and filling a prescription is: Cavia porcellus feedstuff 95.45%, casein 1%, sucrose 1.5%, Adeps Sus domestica 1%, cellulose 1%, cholesterol 0.05%.It is administration that modeling begins, every day 1 time, continuous 8 weeks.Positive control group selection Chinese medicine Fangshi capsule is as positive control drug, every day gavage 3g/kg.Elaeagnus conferta fruits fruit jam is made suspension with 0.5% sodium carboxymethyl cellulose solution.High dose group gavage every day elaeagnus conferta fruits fruit jam suspension 35g/kg (crude drug dosage); Low dose group gavage every day elaeagnus conferta fruits fruit jam suspension 7g/kg (crude drug dosage).Fasting 24h after the last administration, Cavia porcellus eye socket place's injection air is lethal, cuts the abdominal cavity open, block cystic duct with vascular forceps, take off gallbladder, extract the bile censorship and measure calcium ion and cholesterol concentration, and cut gallbladder open, and calculate every composition stone number of elements and gallstone formation rate, the calculus censorship is qualitative.Cholesterol determination enzymic colorimetric, calcium are measured and are stretched the III method with azo.The calculus way of qualitative analysis: get 10mg calculus powder and put into and in vitro add chloroform 10mL, fully mix approximately filter 23 mL after 15min, after adding acetic anhydride 1mL and concentrated sulphuric acid 0.2mL and mixing, put dark place 10min, aobvious aeruginous person is the cholesterol radiopaque calculus.Experimental result is used the SPSS statistical software.Measurement data with
Figure BSA00000869439000422
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the gallstone formation rate of model group, the calcium ion in bile and cholesterol concentration are significantly higher than matched group, prompting stone model modeling success.High dose group can significantly reduce calcium ion and the cholesterol concentration in calculus rate, bile.Experimental result sees Table 54,55.
The impact of table 54 elaeagnus conferta fruits fruit jam on Cavia porcellus cholesterol cholelithiasis
Figure BSA00000869439000431
The impact of table 55 elaeagnus conferta fruits fruit jam on calcium ion and cholesterol concentration in cholesterol cholelithiasis guinea pig bile
Figure BSA00000869439000432
Annotate: compare with model group, *P＜0.05, *P＜0.01
Elaeagnus conferta fruits fruit jam treatment hyperuricemia
Experimental technique:
Get 50 of SD rats (male, body weight 180-220g, the SPF level, laboratory animal resource center of Nat'l Pharmaceutical ﹠ Biological Products Control Institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, low dose group, high dose group.Each is organized rat feeding and all uses normal diet and distilled water.Model group, positive controls, high dose group, four groups of Cavia porcellus Intraperitoneal injection of hypoxanthine every day 1000mg/kg of low dose group.It is administration that modeling begins, once a day, and continuous eight weeks.Positive control group selection Allopurinol Tablets is as positive control drug, every day gavage 3g/kg.Elaeagnus conferta fruits fruit jam is made suspension with 0.5% sodium carboxymethyl cellulose solution.High dose group gavage every day elaeagnus conferta fruits fruit jam suspension 35g/kg (crude drug dosage); Low dose group gavage every day elaeagnus conferta fruits fruit jam suspension 7g/kg (crude drug dosage).After last administration 1h, rat eye socket rear vein beard is taken a blood sample, and blood sample is placed in respectively the centrifuge tube of 1.5mL, and natural blood coagulation 1h under room temperature is at 2500rmin -1Centrifugal 5min gets upper serum, detects assay kit (BioVision company produce) with uric acid and measures Level of Serum Uric Acid.Experimental result is used the SPSS statistical software.Measurement data with X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the serum uric acid concentration of model group is significantly higher than matched group, prompting hyperuricemia model modeling success.High dose group can significantly reduce serum uric acid concentration.Experimental result sees Table 56.
The impact of table 56 elaeagnus conferta fruits fruit jam on the guinea pig serum uric acid concentration
Figure BSA00000869439000442
Annotate: compare with model group, *P＜0.05, *P＜0.01
Embodiment 9
The preparation of elaeagnus conferta fruits fruit juice
Elaeagnus conferta fruits is selected, cleaning, gets 1000g, smashs homogenate to pieces, and is centrifugal through common dewatering centrifuge, isolates upper strata centrifugal liquid and adds adjuvant, mass ratio is as follows: elaeagnus conferta fruits 60%, drinking pure water 37%, xylitol 2.95%, citric acid 0.05%, mix homogeneously, sterilization, canned, make fruit juice.
Elaeagnus conferta fruits fruit juice treatment renal calculus
Experimental technique:
Get 50 of Wistar rats (male, body weight 180-220g, the SPF level, Chinese pharmaceutical biological product identifies that institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.Each is organized rat and all uses normal diet and distilled water to raise.Model group, positive controls, high dose group, four groups of rats of low dose group are only used 1% ethylene glycol (EG)+2% ammonium chloride (AC) gavage every day 2mL/.It is administration that modeling begins, once a day, and continuous eight weeks.Positive control group selection Chinese medicine granules for treating nephrolith is as positive control drug, every day gavage 3g/kg.High dose group gavage every day elaeagnus conferta fruits fruit juice 10g/kg (crude drug dosage); Low dose group gavage every day elaeagnus conferta fruits fruit juice 2g/kg (crude drug dosage).After last administration 1h, use the pentobarbital sodium anesthetized animal, abdominal aortic blood detects blood urea nitrogen, creatinine content, gets kidney and detects Kidney coefficients, gets left kidney and surveys the kidney calcium content, and right kidney is done pathological examination, surveys gallstone formation rate.Experimental result is used the SPSS statistical software.Measurement data with
Figure BSA00000869439000451
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the gallstone formation rate of model group is significantly higher than matched group, and Kidney coefficients is significantly lower than matched group, and kidney calcium content, serum urea nitrogen and serum creatinine are significantly higher than matched group, prompting stone model modeling success.High dose group can significantly reduce the calculus rate, improves Kidney coefficients, reduces kidney calcium content, serum urea nitrogen and serum creatinine.Experimental result sees Table 57,58.
The impact of table 57 elaeagnus conferta fruits fruit juice on the kidney of rats calculus
Figure BSA00000869439000452
The impact of table 58 elaeagnus conferta fruits fruit juice on large mouse with renal calculs Kidney coefficients, kidney calcium content, serum creatinine and serum urea nitrogen
Figure BSA00000869439000453
Figure BSA00000869439000454
Annotate: compare with model group, *P＜0.05, *P＜0.01
Elaeagnus conferta fruits fruit juice treatment bilirubinoid cholelithiasis
Experimental technique:
Get 50 of Cavia porcelluss (female, body weight 250-300g cleans level, and Chinese pharmaceutical biological product identifies that institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.The matched group Cavia porcellus is raised and uses normal diet, all the other are respectively organized the Cavia porcellus raising and use the bilirubinoid cholelithiasis to cause the stone feedstuff, and filling a prescription is: normal diet 95.43%, casein 1%, sucrose 1.5%, Adeps Sus domestica 1%, cellulose 1%, cholic acid 0.02%, cholesterol 0.05%.It is administration that modeling begins, every day 1 time, continuous 8 weeks.Positive control group selection Chinese medicine Fangshi capsule is as positive control drug, every day gavage 3g/kg.High dose group gavage every day elaeagnus conferta fruits fruit juice 10g/kg (crude drug dosage); Low dose group gavage every day elaeagnus conferta fruits fruit juice 2g/kg (crude drug dosage).Fasting 24h after the last administration, Cavia porcellus eye socket place's injection air is lethal, cuts the abdominal cavity open, blocks cystic duct with vascular forceps, takes off gallbladder, extracts bile out with syringe and measures calcium ion (Ca 2+) content, total bilirubin, conjugated bilirubin and calculate unconjugated bilirubin concentration, cut at last gallbladder open, calculate every composition stone number of elements and gallstone formation rate, the calculus censorship is qualitative.Calcium ion content is measured and is used the methyl thymol blue colorimetry.Total bilirubin, calculus determination of bilirubin vanadate oxidizing process.The calculus way of qualitative analysis: get a little calculus powder and put in vitro, add dehydrated alcohol 3mL, add the actual 1mL of freshly prepared diazonium after note, shaking up rear appearance red is that bilirubin is positive.Experimental result is used the SPSS statistical software.Measurement data with
Figure BSA00000869439000461
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the gallstone formation rate of model group, calcium ion content, total bilirubin concentration, unconjugated bilirubin concentration all are significantly higher than matched group, prompting stone model modeling success.High dose group can significantly reduce calculus rate, calcium ion content, total bilirubin concentration and unconjugated bilirubin concentration.Experimental result sees Table 59,60.
The impact of table 59 elaeagnus conferta fruits fruit juice on Cavia porcellus bilirubinoid cholelithiasis
Figure BSA00000869439000462
The impact of table 60 elaeagnus conferta fruits fruit juice on bilirubinoid cholelithiasis Cavia porcellus conjugated bilirubin, unconjugated bilirubin, total bilirubin
Figure BSA00000869439000463
Figure BSA00000869439000464
Figure BSA00000869439000471
Annotate: compare with model group, *P＜0.05, *P＜0.01
Elaeagnus conferta fruits fruit juice treatment cholesterol cholelithiasis
Experimental technique:
Get 50 of Cavia porcelluss (female, body weight 250-300g cleans level, and Chinese pharmaceutical biological product identifies that institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, low dose group, high dose group.The matched group Cavia porcellus is raised and uses normal diet, and all the other are respectively organized the Cavia porcellus raising and use the cholesterol cholelithiasis to cause the stone feedstuff, and filling a prescription is: Cavia porcellus feedstuff 95.45%, casein 1%, sucrose 1.5%, Adeps Sus domestica 1%, cellulose 1%, cholesterol 0.05%.It is administration that modeling begins, every day 1 time, continuous 8 weeks.Positive control group selection Chinese medicine Fangshi capsule is as positive control drug, every day gavage 3g/kg.High dose group gavage every day elaeagnus conferta fruits fruit juice 10g/kg (crude drug dosage); Low dose group gavage every day elaeagnus conferta fruits fruit juice 2g/kg (crude drug dosage).Fasting 24h after the last administration, Cavia porcellus eye socket place's injection air is lethal, cuts the abdominal cavity open, block cystic duct with vascular forceps, take off gallbladder, extract the bile censorship and measure calcium ion and cholesterol concentration, and cut gallbladder open, and calculate every composition stone number of elements and gallstone formation rate, the calculus censorship is qualitative.Cholesterol determination enzymic colorimetric, calcium are measured and are stretched the III method with azo.The calculus way of qualitative analysis: get 10mg calculus powder and put into and in vitro add chloroform 10mL, fully mix approximately filter 23 mL after 15min, after adding acetic anhydride 1mL and concentrated sulphuric acid 0.2mL and mixing, put dark place 10min, aobvious aeruginous person is the cholesterol radiopaque calculus.Experimental result is used the SPSS statistical software.Measurement data with
Figure BSA00000869439000472
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the gallstone formation rate of model group, the calcium ion in bile and cholesterol concentration are significantly higher than matched group, prompting stone model modeling success.High dose group can significantly reduce calcium ion and the cholesterol concentration in calculus rate, bile.Experimental result sees Table 61,62.
The impact of table 61 elaeagnus conferta fruits fruit juice on Cavia porcellus cholesterol cholelithiasis
Figure BSA00000869439000473
Figure BSA00000869439000481
The impact of table 62 elaeagnus conferta fruits fruit juice on calcium ion and cholesterol concentration in cholesterol cholelithiasis guinea pig bile
Figure BSA00000869439000482
Figure BSA00000869439000483
Figure BSA00000869439000484
Annotate: compare with model group, *P＜0.05, *P＜0.01
Elaeagnus conferta fruits fruit juice treatment hyperuricemia
Experimental technique:
Get 50 of SD rats (male, body weight 180-220g, the SPF level, laboratory animal resource center of Nat'l Pharmaceutical ﹠ Biological Products Control Institute provides), be divided at random five groups, i.e. matched group, model group, positive controls, high dose group, low dose group.Each is organized rat feeding and all uses normal diet and distilled water.Model group, positive controls, high dose group, four groups of Cavia porcellus Intraperitoneal injection of hypoxanthine every day 1000mg/kg of low dose group.It is administration that modeling begins.Fed for eight weeks continuously.Positive control group selection Allopurinol Tablets is as positive control drug, every day gavage 3g/kg.High dose group gavage every day elaeagnus conferta fruits fruit juice 10g/kg (crude drug dosage); Low dose group gavage every day elaeagnus conferta fruits fruit juice 2g/kg (crude drug dosage).After last administration 1h, rat eye socket rear vein beard is taken a blood sample, and blood sample is placed in respectively the centrifuge tube of 1.5mL, and natural blood coagulation 1h under room temperature is at 2500rmin -1Centrifugal 5min gets upper serum, detects assay kit (BioVision company produce) with uric acid and measures Level of Serum Uric Acid.Experimental result is used the SPSS statistical software.Measurement data with
Figure BSA00000869439000485
X2 check and variance analysis are adopted in expression.
Experimental result:
Compare with matched group, the serum uric acid concentration of model group is significantly higher than matched group, prompting hyperuricemia model modeling success.High dose group can significantly reduce serum uric acid concentration.Experimental result sees Table 63.
The impact of table 63 elaeagnus conferta fruits fruit juice on the guinea pig serum uric acid concentration
Figure BSA00000869439000491
Annotate: compare with model group, *P＜0.05, *P＜0.01
Content of the present invention includes but not limited to specific embodiment.Although herein disclosed is some specific preferred embodiment, those skilled in the art should be obvious from foregoing disclose, can modify and change these embodiment and method under the prerequisite that does not deviate from spirit and scope of the invention.

Claims (8)

1. the purposes of an elaeagnus conferta fruits is characterized in that: described purposes is that elaeagnus conferta fruits is for the preparation of the application of the medicine for the treatment of renal calculus, cholelithiasis and hyperuricemia.
2. the purposes of a kind of elaeagnus conferta fruits according to claim 1, is characterized in that: the application that described treatment renal calculus refers to reduce kidney stone rate, raising Kidney coefficients, reduction kidney calcium content, reduction serum urea nitrogen and reduces the medicine of serum creatinine.
3. the purposes of a kind of elaeagnus conferta fruits according to claim 1, it is characterized in that: described treatment cholelithiasis refers to treat bilins cholelithiasis and cholesterol cholelithiasis.
4. the purposes of a kind of elaeagnus conferta fruits according to claim 3, is characterized in that: preferred therapeutic bilirubinoid cholelithiasis in described treatment bilins cholelithiasis.
5. the purposes of a kind of elaeagnus conferta fruits according to claim 4, it is characterized in that: described treatment bilirubinoid cholelithiasis refers to reduce calcium ion content, total bilirubin concentration and the unconjugated bilirubin concentration in cholelithiasis rate, bile.
6. the purposes of a kind of elaeagnus conferta fruits according to claim 3, it is characterized in that: described treatment cholesterol cholelithiasis refers to reduce the cholesterol level in cholelithiasis rate and bile.
7. the purposes of a kind of elaeagnus conferta fruits according to claim 1, it is characterized in that: described treatment hyperuricemia refers to reduce serum uric acid.
8. the purposes of a kind of elaeagnus conferta fruits product according to claim 1, it is characterized in that: the purposes of described elaeagnus conferta fruits can also extend to the Related product that product is elaeagnus conferta fruits, comprise elaeagnus conferta fruits fruit water extract, dehydration elaeagnus conferta fruits, capsule, tablet, granule, fruit vinegar, fruit jam and fruit juice, described purposes is the described purposes of claim 1-7.
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106666586A (en) * 2016-09-13 2017-05-17 安徽宝恒农业有限公司 Elaeagnus conferta flavor indochina dragonplum fruit jelly
CN108936180A (en) * 2018-08-15 2018-12-07 黄清华 Sour mew-open country banana compound solid beverage and wild banana extract production method
CN112826060A (en) * 2021-01-20 2021-05-25 腾冲市通三泰农业发展有限公司 Elaeagnus conferta jam and preparation method thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1919056A (en) * 2006-09-26 2007-02-28 北京理工亘元医药技术开发中心有限公司 Processing and use of foodstuff or health food comprising elaeagnus conferta fruits

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1919056A (en) * 2006-09-26 2007-02-28 北京理工亘元医药技术开发中心有限公司 Processing and use of foodstuff or health food comprising elaeagnus conferta fruits

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106666586A (en) * 2016-09-13 2017-05-17 安徽宝恒农业有限公司 Elaeagnus conferta flavor indochina dragonplum fruit jelly
CN108936180A (en) * 2018-08-15 2018-12-07 黄清华 Sour mew-open country banana compound solid beverage and wild banana extract production method
CN112826060A (en) * 2021-01-20 2021-05-25 腾冲市通三泰农业发展有限公司 Elaeagnus conferta jam and preparation method thereof

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