CN1047191A - Phytohygiene disinfectant and uses thereof - Google Patents
Phytohygiene disinfectant and uses thereof Download PDFInfo
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- CN1047191A CN1047191A CN90104130A CN90104130A CN1047191A CN 1047191 A CN1047191 A CN 1047191A CN 90104130 A CN90104130 A CN 90104130A CN 90104130 A CN90104130 A CN 90104130A CN 1047191 A CN1047191 A CN 1047191A
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- acid
- disinfectant
- vitamin
- phytohygiene
- plant
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/141—Feedstock
- Y02P20/145—Feedstock the feedstock being materials of biological origin
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
A kind of phytohygiene disinfectant has been described, it contains I) at least two seed amino acids and/or natural carboxylic acid or their derivative or salt, II) purine and/or pyrimidine or derivatives thereof, and III) vitamin or its derivative or parent, wherein: I) a kind of particular compound of group is 0.1 to 10 weight portion, II) a kind of particular compound of group is 0.001 to 1 weight portion, and III) a kind of particular compound of group is 0.001 to 1 weight portion.This medicament possibility that is widely used.Be preferred for preventing and prevent and treat plant disease that biology causes and the output of raising and stable cultivated plant.Especially with the extract of tunning (for example mixed fertilizer) when shared, its effect is stronger.In described range of application,, also can reach the effect of being pursued if in described composition, only contain a seed amino acid.
Description
The outstanding characteristics of the mixed fertilizer that microbial material is formed are that various microorganisms are arranged, its metabolism product and other organic and inorganic compound.For a long time, except as organic manure, it may have some favorable influence to the health of plant.So, the extract of bark mixed fertilizer should have the effect of a kind of bactericide and bacteriostatic agent to the Phytophthora kind of various soil existence.The mixed fertilizer extract generally is the tunning that is obtained by plant and vegetable remains thing, animal residue, brown seaweed or its mixture.
The extract of known fermented organic or press water are used for the fruit of the green portion of plant such as leaf and stem, plant and plant the period of the day from 11 p.m. to 1 a.m, can give play to a kind of phytohygiene effect.People such as H.C.Welt-zien (Braunschwieg), have reported this effect later on for 39,1987,25 pages at " German plant protection bulletin ".After this result of the test of carrying out proved repeatedly estimate, mixed fertilizer and mixed fertilizer extract be to the positive role that health had of plant.The fact shows that extract has the resistant effect of bringing out and/or directly influences pathogene handled plant.
In addition, the required extra-nutrition thing that various mineral matters and carbon, nitrogen and energy source are helped out of many microorganisms is also open.These extra-nutrition things belong to the solvent of cell, can not be synthetic by microorganism by simple constituent.What belong to this class extra-nutrition thing has amino acid, carboxylic acid, purine, pyrimidine, carbohydrate and vitamin.Can distinguish extra-nutrition thing and nutrients significantly from its function and concentration.
By the above-mentioned possibility that mixed fertilizer and mixed fertilizer extract reduce, get rid of or avoid plant and plant part to fall ill, so far can not be satisfactory.
Known various compounding substances has the phytohygiene effect.SU1446155A has described a kind of preparation that contains amino acid, purine and a kind of vitamin.Said preparation is as the additive of tissue culture nutrient solution, with the regeneration of realization plant tissue and the reinforcement of seedling.Do not mention and use it for plant protection and be used for ground plant part.EP-A-0253714 includes the two-component mixture of bactericide effect, and it is made up of a kind of pyrimidine derivatives and alanine or its a kind of derivative.EP-A-0044224 has proposed to make chlorocholine chloride and " metabolism additive " (also having vitamin, amino acid, purine, pyrimidine, carboxylic acid) to combine.Itself does not have bactericidal action chlorocholine chloride and metabolism additive.DD-A-63669 relates to a kind of system bactericide.It contains a glycine derivative and 0.02 to 2 part of N
6A kind of bond that the adenine that replaces forms.An a kind of purine (adenine) and a seed amino acid (glycine) have only been considered.WO79/00838 relates to the chemicals that can be used for agricultural, for example bactericide, weed killer herbicide and plant growth regulator have wherein been considered in the following additive one or more: carbohydrate, organic acid (especially fatty acid), vitamin and coenzyme, purine and pyrimidine nucleoside and nucleotide, naturally occurring fat and oil, some amino acid and conditioning agent (when agricultural chemicals itself is not plant growth regulator).DE-A-2625398 relates to a kind of preparation and method that stimulates potato sprout, and wherein said preparation must comprise a kind of amino acid and a kind of N of cysteine form
6The purine of furfuryladenine form.
In order to develop application possibility the most widely, above-mentioned preparation does not propose clearly design, just as a kind of phytohygiene disinfectant constitutes very prevailingly.The possibility that this is equally applicable to discuss promptly reduces, gets rid of or avoids plant and plant part disease by mixed fertilizer or mixed fertilizer extract.Thereby task of the present invention is this is remedied.
Solution of the present invention is a kind of phytohygiene disinfectant, it is characterized in that: contain I) natural carboxylic acid or its esters and/or at least two seed amino acids or its esters,
II) purine and/or its derivative, particularly nucleosides, nucleotide and its esters, and/or pyrimidine and/or its derivative, particularly nucleosides, nucleotide and its esters, and the salt of purine and pyrimidine and (III) vitamin or its derivative or parent,
Wherein, I) a kind of particular compound of group accounts for about 0.1 to 10 weight portion,
II) Zu a kind of particular compound accounts for about 0.001 to 1 weight portion,
III) Zu a kind of particular compound accounts for about 0.001 to 1 weight portion,
The core concept of phytohygiene disinfectant of the present invention is, at least adds two seed amino acids or its esters in the composition of qualitative and quantitative description in the above.Wherein preferentially select three, four or more kinds of amino acid or its esters for use.Thereby for this disinfectant with phytohygiene effect of looking for has been opened up application possibility the most widely.The fact shows, in some application-specific, may not request two or more amino acid at least, also will explain in detail this below.
At least two seed amino acid merger are gone in the phytohygiene disinfectant of the present invention, and the effect of being brought can be made description below technically:
Such disinfectant is because the promotion of non-pathogenic microorganism is caused for the phytohygiene effect of blade surface.The microorganism of this tool facilitation or suppress or directly suppress pathogene fungi or bacterium by striving food indirectly by antagonism, and play the phytohygiene effect thus.As the microorganism that has a positive effect as far as possible, known have for example pseudomonas and Bacillus class and a various saccharomycete.Also can consider countless other microorganisms.These microorganisms have different nutritional requirements, thereby also will consider different amino acid by different amino alcohols is provided in corresponding preparation, and particularly two or more amino acid can adapt to its nutritional requirement.It is not the nutritional need of only in a plant is caused a disease system, considering the microorganism of determining under the situation of disease worm biological nutrition requirement that all have a positive effect to blade surface (leaf-head).The determining of nutrient combination promoting antagonism and competition as well as possiblely and avoiding promoting pathogene fungi and bacterium a kind of the trading off between the two as far as possible.The amino acid whose number of proteinogen (21) is far longer than the number (11) of vitamin (11), purine (4), pyrimidine (3) or the carboxylic acid of particular importance, if do not consider parent and derivative.Two or more amino acid probability preferably are far longer than vitamin, purine, pyrimidine or carboxylic acid.Learn also that in addition the microorganism of blade surface and plant live in the narrower biocoenosis.But plant drains amino acid consumingly, drains not only by root, and by leaf.In the progressively procedure of adaptation of microorganism and blade surface specific condition, amino acid plays an important role.The microorganism that can get the leafing table thus needs the conclusion of specific amino acids.
Content of the present invention is also particularly including the fruit of handling ground plant part and results with the phytohygiene disinfectant of foregoing invention, particularly is used for preventing and preventing and treating the plant disease that biology causes, to guarantee and to improve output.As previously mentioned, in this application, advantageously mix the tunning that uses plant and plant residue, brown seaweed, animal residue or its mixture with bactericide of the present invention, wherein enrichment microorganism, particularly various bacteriums, saccharomycete, fungi and unicellular alga.In this favourable application possible range (being with or without tunning), if corresponding medicament is used to prevent and treat contagiosity Phytophthora, grey matter Botrytis, viticolous Plasmopara, grass family Erysiphe, Uncinula Necator, Tilletia kind (sp,) and the false Peziza of tracheiphila, the present invention will be valuable especially.
If tunning is mixed use with phytohygiene disinfectant of the present invention, the ratio of Hun Heing will not play a decisive role at this so.As rough principle, to do matter, mixed proportion can be about 1 weight portion phytohygiene disinfectant of the present invention to 100 to 10000, particularly the tunning of 300 to 3000 weight portions.
In aforesaid useful range of application, promptly be used to handle the fruit of ground plant part and results, in particular for preventing and preventing and treating the biological plant disease that causes to guarantee and raising output, and be used from tunning and similar substance one and prevent and treat the contagiosity Phytophthora, the grey matter Botrytis, viticolous Plasmopara, the grass family Erysiphe, the Uncinula Necator, the false Peziza of Tilletia kind (sp.) and tracheiphila, experiment shows, if abandon foregoing two or more the amino acid whose requirements at least that in phytohygiene disinfectant of the present invention, add, and make phytohygiene disinfectant of the present invention only contain a seed amino acid or its esters, also can reach gratifying effect a bit.Therefore, content of the present invention also comprises aforementioned by components I), II) and III) a kind of phytohygiene Application of disinfectants of forming, wherein only add a seed amino acid or its esters.
According to above-mentioned range of condition, implement preferred following mode when of the present invention:
If in phytohygiene disinfectant of the present invention, have only a kind of I) particular compound of group, at this moment, will be scheduled to its consumption, consider above-mentioned range of condition on this basis, determine II) with III) application quantity mutually of group particular compound.If comprise I) the multiple compound of family, then advise based in I) weight particular compound placed in the middle in the group.For this situation, promptly contain two or more I in the invention phytohygiene disinfectant) when organizing compound, be that the basis is favourable with its mean value.II) and III) every kind of particular compound of family in above-mentioned given quantitative range with I as benchmark) the group consumption is associated.That is to say, for example, for a kind of I of 0.1 to 10 weight portion scheduled volume) the group compound, II) a kind of particular compound A of group) consumption should be 0.001 to 1 weight portion, a kind of particular compound B) consumption should be 0.001 to 1 weight portion, the rest may be inferred.
I as benchmark) preferred 0.1 to 5 weight portion of group particular compound, preferred especially about 0.5 to 5 weight portion.Preferred following amino acid: L-aspartic acid, L-glutamic acid, L-histidine, L-arginine, L-alanine, L-serine, D-and/or L-tryptophan, L-tyrosine, D-and/or L-phenyl alanine, altheine, L-lysine, L-methionine, L-threonine, D-and/or L-glutamine, L-proline, L-hydroxy-proline, D-and/or L-valine, L-leucine, L-glycine, L-cystine and L-isoleucine.Adding under the situation of carboxylic acid, disinfectant of the present invention preferably contains at least a in following natural carboxylic acid or its esters: pyruvic acid, lactic acid, oxalic acid, malic acid, succinic acid, malonic acid, propionic acid, fumaric acid, α-Tong Wuersuan, glutaric acid and maleic acid.
Advantageously select a kind of purine or pyrimidine, make this phytohygiene disinfectant contain following a kind of compound at least, its form is adenine, guanine and hypoxanthine (purine), cytimidine, thymidine and uracil (pyrimidine) or derivatives thereof, particularly nucleosides, nucleotide and its esters.
The best mode that adds vitamin is to select at least a vitamin as described below: thiamine, vitamin h, nicotinic acid, calcium pantothenate, Cobastab
6Hydrochloride, vitamin b3, p-aminobenzoic acid, retinol palmitate (vitamin A), choline, vitamin b23 (vitamin 12) and folic acid and derivative, salt or parent.Preferred vitamin B
6Hydrochloride, vitamin b3, retinol palmitate, choline and the each consumption of these vitamins of folic acid are 0.0001 to 1mg/l, 0.06mg/l particularly, the each consumption of these things of supporting one's family of thiamine hydrochloride, nicotinic acid and calcium pantothenate is about 0.05mg/l, vitamin p-aminobenzoic acid consumption is about 0.012mg/l, vitamin vitamin b23 consumption is that about 0.012mg/l and vitamins biotin consumption are about 0.0006mg/l.
When using phytohygiene disinfectant of the present invention, preferentially select moisture form for use.Wherein preferred following concentration range: in every liter of moisture medicament, the liquor-saturated each consumption of at least a concrete amino acid or carboxylic is about 0.01 to 10mg/l, particularly about 0.1 to 1mg/l, each consumption of at least a concrete purine or pyrimidine is about 0.0001 to 1mg/l, particularly 0.006mg/l and the each consumption of at least a concrete vitamin are about 0.0001 to 1mg/l.Phytohygiene disinfectant of the present invention also can advantageously constitute in the following manner, the consumption of wherein concrete purine or pyrimidine is about 0.001 to 0.1mg/l, preferred especially 0.006mg/l, concrete amino acid or natural carboxylic acid's consumption is about 0.1 to 1mg/l, preferred especially 0.3mg/l, concrete vitamin, Cobastab
6Hydrochloride, vitamin b3, retinol palmitate, choline and folic acid consumption are about 0.0002 to 1mg/l, 0.06mg/l particularly, concrete vitamin thiamine hydrochloride, nicotinic acid and calcium pantothenate consumption are about 0.05mg/l, vitamin p-aminobenzoic acid consumption is about 0.012mg/l, vitamin vitamin b23 consumption is about 0.0012mg/l and the vitamins biotin consumption is about 0.0006mg/l.
Under concrete condition, be preferably the phytohygiene disinfectant is incapsulated use.To capsule wall without any restriction.As long as environment can be accepted, and under water function, the phytohygiene disinfectant that entrains into can flow out by metering and get final product.Can make it be diffused into active position exactly thus, so do not make its loss function to the place diffusion of plant species not.By incapsulating, maintenance effect performance for a long time.As examples of suitable, can exemplify the capsule of making by liposome material such as phosphatide.
The present invention can be particularly advantageous for handling plant and plant part, especially for prevention and the biological plant disease that causes of control.As the plant disease of particular importance, can exemplify contagiosity Phytophthora (potato cauline leaf blight), grey matter Botrytis (grey matter mould), viticolous Plasmopara (the false rust staining disease of radish), Uncinula Necator (the true rust staining disease of radish), grass family Erysiphe (the true rust staining disease of cereal), (raw meat is deceived powder to stinking smut of wheat
The Pseudomonas kind) and trachejphila
Blue (the red charcoal of grape that belongs to of dish
Sick).If not for the phytohygiene purpose, and just to the results that guarantee and improve economic crops, it also is favourable adding phytohygiene disinfectant of the present invention under specific circumstances.
Under concrete condition, phytohygiene disinfectant of the present invention is used in combination with the mix extract and can shows and strengthen the effect cure the disease and to suppress disease and raising and stable harvest.Wherein, can after obtaining watery mixed fertilizer extract, make itself and watery directly mixing mutually of phytohygiene disinfectant of the present invention, directly use then.Also can make the freeze drying of mixed fertilizer extract, it is mixed mutually with the phytohygiene disinfectant of the present invention of powder-form.Here, the ratio of mixing is not strict.Also have other may utilize the acting in conjunction of mixed fertilizer extract and phytohygiene disinfectant of the present invention.As in leaching process, phytohygiene disinfectant of the present invention being added in the organic substance of fermentation, can accomplish this point.Passing along with extraction time, some decomposition or conversion have taken place in the component in the phytohygiene disinfectant of the present invention, so, obtain in this way and the final extract that adds on forming with different originally, only had phytohygiene disinfectant of the present invention originally.Although form to change, still can obtain biochemistry effect identical when adding phytohygiene of the present invention disappears female agent separately.It is more effective whether independent adding phytohygiene disinfectant of the present invention combines than phytohygiene disinfectant/mixed fertilizer extract, decide according to concrete condition.In general, phytohygiene disinfectant of the present invention has diversified adding possibility.
Under concrete condition, which kind of medicament has optimum efficiency, an available simple preliminary test explanation.Wherein, the host of employing/parasite system plays an important role.Under concrete condition, any advantageous particularly of aforementioned three kinds of situations (control is fermented, used simultaneously and use separately with the mixed fertilizer extract), available a kind of screening technique commonly used is determined, wherein corresponding harmful pathogenic microbes or artificial cultivation plant is tested.Subjects is the plant to be protected that has harmful pathogenic microbes.
Can prepare mixed fertilizer extract/phytohygiene antiseptic composition by following method: the aqueous solution (total about 0.3mg active principle in every premium on currency) the pouring one weight portion mixed fertilizer with 10 weight portions phytohygiene disinfectant of the present invention can be prepared the mixed fertilizer of any type in principle.Commercially available mixed fertilizer is particularly suitable also, as the product " Biokuh-dung " of Backhus company.After being poured into together, to shake once in the mixture of phytohygiene disinfectant and mixed fertilizer.Temperature is preferably about 18 to 25 ℃ in the leaching process.According to host/parasite system, sustainable 1 to 30 day of extraction time.After extract finishing, other has the filter filtration (not being the aseptic filtration method) in similar aperture with a filter cloth or one.Can directly use behind this extracting liquid filtering, perhaps by freeze drying or other method preservation and preservation.The professional when obtaining mixed fertilizer extract/phytohygiene disinfectant, not significantly restriction basically.Above-mentioned process can be carried out multiple modification.
By adding specific invention phytohygiene disinfectant, and interrelate, on purpose promote the microorganism that plays an important role might the multiple microorganism in growing in mixed fertilizer with the mixed fertilizer extract.Can in leaching process, control fermentation process in this way, or carry out reissuing ferment after a kind of.Phytohygiene disinfectant of the present invention combined separately or with the mixed fertilizer extract be directly used in blade (or other plant part), produce the phytohygiene effect in the following manner: a) on purpose promote antagonism and/or competition, and suppress harmful pathogenic microbes thus; B) bring out resistance, wherein directly make inducing agent, or make inducing agent with the metabolite of promotion microorganism with phytohygiene disinfectant of the present invention; And c) disturbs the recognition mechanism that is harmful between pathogenic microbes and the useful plant by biochemistry or physical reactions.Can be with moisture form itself, combine with moisture mixed fertilizer extract or mixed fertilizer press water, combine its adding with the mixed fertilizer lyophilized products and the analog that suspend again.The disinfectant particularly suitable imposes on ground plant part or the seed or imposes on underground plant part.Handle kind of a period of the day from 11 p.m. to 1 a.m and can adopt following insecticide-applying way: wash seed with treatment media, with the mixed fertilizer extract, have the invention phytohygiene Slerilant mixture coating of carrier, perhaps incapsulate in described mode.
In host/parasite system, make the grey matter Botrytis reduce by 60% with a kind of pure mixed fertilizer extract to fabaceous invasion and attack, with mixed fertilizer extract and phytohygiene disinfectant of the present invention combine (obtaining adding the phytohygiene disinfectant behind the mixed fertilizer extract) then will attack reduction by 80%.The analog value of the host of contagiosity Phytophthora-tomato-parasite system be 80% or the 90%(laboratory test).Handle following host-parasite system with mixed fertilizer extract of the present invention/phytohygiene antiseptic composition: Fragaria-grey matter Botrytis, potato-contagiosity Phytophthora, Hordeum-grass family Erysiphe, Vitis-grape overgrow Plasmopara and Vitis-Uncinula Necator, compare with untreated system, invasion and attack reduction value is respectively 50%, 85%, 50 to 60%, 76 to 96% or 70 to 80%, harvest improves 20 to 40%, is 95% for host-parasite system Fragaria-grey matter Botrytis and potato-contagiosity Phytophthora.Wherein, host-parasite system Fragaria-grey matter Botrytis, potato-contagiosity Phytophthora and the Vitis-grape Plasmopara of overgrowing is to test garden test in the open, and other host-parasite system is then tested in the greenhouse.
The result proves, the advantage that the present invention has is, protected the plant of being handled or plant part such as seed and fruit not to be subjected to affect, suppresses under the situation that does not add suspicious chemical insecticide in other words or killed harmful pathogenic microbes.Under specific circumstances, can confirm that also it is to animal pest spider Acarina) the expulsion effect arranged.Also can be quantitatively and improve the harvest of cultivated plant qualitatively with preparation of the present invention." plant " this notion should interpreted in its broadest sense, ie at this, comprises that live and dead plant or plant part, as blade, stem, fruits and seeds and underground organization and available timber etc. for example.
Further explain the present invention by means of various embodiment below.
Embodiment
The purpose of test, be to see phytohygiene mixture of the present invention itself or extract liquid phase with mixed fertilizer and mix, tomato) and grey matter Botrytis (grey matter mould) (plant: affect Phaseolus) when plant is subjected to attacking, whether can resist contagiosity Phytophthora (potato cauline leaf blight) (plant: effectively.Wherein, in both cases, starting point is a following plants health mixture:
1) the phytohygiene disinfectant solution of the present invention that, has the effect of infectivity resistant Phytophthora:
L-aspartic acid 300 μ g/l
L-glutamic acid 300 μ g/l
L-histidine 300 μ g/l
L-arginine 300 μ g/l
Altheine 300 μ g/l
L-lysine 300 μ g/l
L-methionine 300 μ g/l
L-threonine 300 μ g/l
D-, L-glutamine 300 μ g/l
L-proline 3 00 μ g/l
L-hydroxy-proline 300 μ g/l
Uracil 6 μ g/l
Hypoxanthine 6 μ g/l
Vitamin b3 60 μ g/l
Retinol palmitate 60 μ g/l
Choline 60 μ g/l
Folic acid 60 μ g/l
P-aminobenzoic acid 12 μ g/l
Vitamin b23 12 μ g/l
2) have the phytohygiene disinfectant in moisture mixed fertilizer extract of infectivity resistant Phytophthora effect:
L-aspartic acid 300 μ g/l
L-glutamic acid 300 μ g/l
L-histidine 300 μ g/l
L-arginine 300 μ g/l
D-, L valine 300 μ g/l
L-leucine 300 μ g/l
L-cystine 300 μ g/l
L-isoleucine 300 μ g/l
Adenine sulfate 6 μ g/l
Guanine hydrochloride 6 μ g/l
Cytimidine 6 μ g/l
Thymidine 6 μ g/l
Thiamine hydrochloride 50 μ g/l
Vitamin h 0.6 μ g/l
Nicotinic acid 50 μ g/l
Calcium pantothenate 50 μ g/l
Cobastab
6Hydrochloride 60 μ g/l
3) have the phytohygiene disinfectant in moisture mixed fertilizer extract of anti-grey matter Botrytis effect:
L-aspartic acid 300 μ g/l
L-glutamic acid 300 μ g/l
L-histidine 300 μ g/l
L-arginine 300 μ g/l
D-, L valine 300 μ g/l
L-leucine 300 μ g/l
L-cystine 300 μ g/l
L-isoleucine 300 μ g/l
Adenine sulfate 6 μ g/l
Guanine hydrochloride 6 μ g/l
Born of the same parents' purine 6 μ g/l
Thymus gland purine 6 μ g/l
Thiamine hydrochloride 50 μ g/l
Vitamin h 0.6 μ g/l
Nicotinic acid 50 μ g/l
Calcium pantothenate 50 μ g/l
Cobastab
6Hydrochloride 60 μ g/l
4) the phytohygiene disinfectant in moisture mixed fertilizer extract of Plasmopara and the effect of Uncinula plate mouth line mouth genus of overgrowing of the grape with anti-Vitis:
L-aspartic acid 300 μ g/l
L-glutamic acid 300 μ g/l
L-histidine 300 μ g/l
L-arginine 300 μ g/l
D-, L valine 300 μ g/l
L-leucine 300 μ g/l
L-cystine 300 μ g/l
L-isoleucine 300 μ g/l
Adenine sulfate 6 μ g/l
Guanine hydrochloride 6 μ g/l
Born of the same parents' purine 6 μ g/l
Thymus gland purine 6 μ g/l
Vitamin b3 60 μ g/l
Retinol palmitate 60 μ g/l
Choline 60 μ g/l
Folic acid 60 μ g/l
P-aminobenzoic acid 12 μ g/l
Vitamin b23 12 μ g/l
Obtain blade as follows: in the greenhouse, cultivate bush bean C.V.Marona.Estimate to want about 14 days that for use initial blade in test secondary vane is then wanted about 3 to 4 weeks.After this single cutting-out is old blade equally, and puts into the bowl of the blotting paper of a useful water-soaked.The blade surface adiabatic drying of beans leaf is a particular importance.The used time of the cultivation of tomato plants is much longer, is about for 5 to 6 weeks.
With a pump spray sporangia suspension of a kind of 70000/ml is sprayed onto the blade downside, tomato leaf is inoculated.Make after the inoculation temperature drop to 12 to 14 ℃ about 12 to 24 hours, thereby to guarantee that zoospore digs into and reaches the infection effect from wound better.Then before evaluation, make temperature be 20 ℃ by day with a light/blackout parallel operation, be 17 ℃ night, so keeps more than 7 days.Carry out the Botrytis inoculation when being coated with extract.Here, in the mixed fertilizer extract/phytohygiene disinfectant of 0.015ml, add a kind of spore suspension of same quantity (in the 0.5-15 malt extracted solution 10
6/ ml).
Obtain extract as follows: the phytohygiene disinfectant that will be referred to (moisture form) was by dilution in 1: 10.Solution with the dilution of 10 weight portions waters on a mixed fertilizer.After pouring into, mixture is shaken once, after this acutely shake once weekly.The temperature and the lighting condition that extract are corresponding with experimental condition.Filter the mixture that stirring just finishes with a filter cloth or coffee filter, make the extract of handling institute's necessary amounts.
In order to comprise the partial action of inductive resistance, intact plant pure phytohygiene disinfectant and extract/phytohygiene Slerilant mixture have also been used.For this reason, with extract phytohygiene disinfectant 2) spray tomato plants adrip, on leaf-head, survive well and settle down in order to ensure the microorganism that occurs thus, make it in the very high phytotron of air humidity, preserve three days (20 ℃ of daytimes, 18 ℃ of nights; 7 to 20 hour interlunation).Thing in contrast, once use water treatment (handling Botrytis) with the 0.5-1% malt extracted solution, when microorganism is Phytophthora, use fresh phytohygiene disinfectant 1 again) and 2) handle in contrast, of the direct influence of phytohygiene disinfectant can be estimated thus for disease progression.
With phytohygiene disinfectant 1) and 2) evaluate with a score of representing with percentage on the tomato leaf handled, wherein, with the digital supplementary means of the score of a C.James (Canadian plant disease diagnosis, 51,1971) as evaluation.(plant: beans), blade pathology size and amplification thereof exceeded the drop boundary and evaluate when microorganism was Botrytis.In order to convert, carried out a variable analysis and Duncan test.
The result
Phytophthora test (plant: tomato)
Mixed fertilizer/phytohygiene Slerilant mixture (mixture 2)) and pure phytohygiene disinfectant 1) shown good anti-Phytophthora effect.The performance that has surpassed pure mixed fertilizer extract significantly.Umerical performance situation is as follows:
Untreated tomato leaf has 80% invasion and attack that are subjected to the Phytophthora blight.Pure mixed fertilizer extract itself makes invasion and attack reduce by 80%, pure phytohygiene disinfectant 1) and disinfectant 2) invasion and attack are reduced to 5%.
Botrytis test (plant: beans)
Here, the phytohygiene disinfectant 3) have a good especially performance.Agent 3) mixture with the mixed fertilizer extract makes invasion and attack reduce by 85%, and mixed fertilizer extract itself can only reduce by 60% with the Botrytis invasion and attack.
Phytophthora test (plant: potato, site test)
The nursery is with the dispenser of spraying processing mode in the open, and during plant growing, per 7 to 10 days repeat once.
Phytohygiene disinfectant 1) performance and pure mixed fertilizer extract are similar, approximately reduce by 45% Phytophthora invasion and attack.Mixed fertilizer extract and phytohygiene disinfectant 2) mixture make invasion and attack reduced by 80%.
The field test that grape is carried out
In grape experiment, phytohygiene disinfectant 4) shown excellent function.Spraying grape with this medicament makes the invasion and attack of true powder mildew fungi reduce more than 50%.Draw the obviously reduction about 30% of invasion and attack of this disease by the scoring of red anthracnose invasion and attack.
Claims (14)
1, the phytohygiene disinfectant is characterized in that, contains
I) natural carboxylic acid or its esters and/or at least two seed amino acids and its esters,
II) purine and/or its derivative and/or pyrimidine and/or its derivative and
III) vitamin or derivatives thereof or parent,
Wherein, its content is:
I) a kind of particular compound 0.1-10 weight portion of group
II) a kind of particular compound 0.001-1 weight portion of group
III) a kind of particular compound 0.001-1 weight portion of group.
2, according to the disinfectant of claim 1, it is characterized in that, contain at least three or four seed amino acid or its esters.
3, according to the disinfectant of claim 1, it is characterized in that 1) group a kind of particular compound amount be 0.5 to 5 weight portion.
4, according to claim 1,2 or 3 disinfectant, it is characterized in that, contain in following amino acid or its esters at least a: L-aspartic acid, L-glutamic acid, L-histidine, L-arginine, L-alanine, L-serine, D-and/or L-tryptophan, L-tyrosine, D-and/or L-phenyl alanine, altheine, L-lysine, L-methionine, L-threonine, D-and/or L-glutamine, L-proline, L-hydroxy-proline, D-and/or L-valine, L-leucine, L-glycine, L-cystine and L-isoleucine.
5, according to the disinfectant one of in the claim 1 to 4, it is characterized in that, contain in following natural carboxylic acid or its esters at least a: pyruvic acid, lactic acid, oxalic acid, malic acid, succinic acid, malonic acid, propionic acid, fumaric acid, α-Tong Wuersuan, glutaric acid and maleic acid.
6, according at least one disinfectant in the claim 1 to 5, it is characterized in that, contain at least a compound of following form: adenine, guanine and hypoxanthine (purine) or cytimidine, thymidine and uracil (pyrimidine) or its esters.
7, according at least one disinfectant in the claim 1 to 6, it is characterized in that, contain at least a vitamin of following form: thiamine, vitamin h, nicotinic acid, calcium pantothenate, pyridoxine, vitamin b3, p-aminobenzoic acid, retinol palmitate, choline, vitamin b23 (Cobastab
12) and folic acid, with and derivative and parent.
8, according to the disinfectant of claim 7, it is characterized in that, exist with moisture form.
9, disinfectant according to Claim 8, it is characterized in that, contain 0.01 to the 10mg/l(preferred 0.1-1mg/l that has an appointment in every liter of moisture medicament) concrete amino acid (at least two seed amino acids), about 0.0001 to 1mg/l at least a concrete purine or pyrimidine and about 0.0001 to 1mg/l at least a concrete vitamin.
According to the disinfectant of claim 9, it is characterized in that 10, the amount of concrete purine or pyrimidine is about 0.001 to 0.1mg/l, preferred 0.006mg/l, concrete amino acid or natural carboxylic acid's amount is about 0.1 to 1mg/l, preferred 0.3mg/l, concrete vitamin, Cobastab
6The amount of hydrochloride, vitamin b3, retinol palmitate, choline and folic acid is about 0.0002 to 1mg/l, preferred 0.06mg/l, the amount of concrete vitamin thiamine hydrochloride, nicotinic acid and calcium pantothenate is about 0.05mg/l, the amount of vitamin p-aminobenzoic acid is about 0.012mg/l, the amount of vitamin vitamin b23 is about 0.0012mg/l, and vitamins biotin is about 0.0006mg/l.
11, aforesaid right is required at least one described disinfectant be used to handle the product of plant part and results on the ground, especially for prevention and the biological plant disease that causes of control, to improve and stable harvest.
12, the purposes of the described disinfectant of claim 11, the tunning comprising plant and vegetable remains thing, brown seaweed, animal residue or its mixture is rich in microorganism, particularly bacterium, saccharomycete, fungi and unicellular alga in the tunning.
13,, comprise in the phytohygiene disinfectant, adding a kind of unique amino acid or the possibility of its a kind of salt according to the purposes of claim 11 or 12.
14,, be used to prevent and treat contagiosity Phytophthora, grey matter Botrytis, the grape false Peziza of Plasmopara, grass family Erysiphe, Uncinula Necator, Tilletia kind (sp.) and tracheiphila of overgrowing according to the purposes one of in the claim 11 to 13.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE3910061A DE3910061A1 (en) | 1989-03-28 | 1989-03-28 | PHYTOSANITANE MEDICINE AND ITS USE |
| DEP3910061.8 | 1989-03-28 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1047191A true CN1047191A (en) | 1990-11-28 |
Family
ID=6377335
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN90104130A Pending CN1047191A (en) | 1989-03-28 | 1990-03-28 | Phytohygiene disinfectant and uses thereof |
Country Status (13)
| Country | Link |
|---|---|
| EP (1) | EP0422147A1 (en) |
| JP (1) | JPH03505585A (en) |
| KR (1) | KR920700002A (en) |
| CN (1) | CN1047191A (en) |
| AU (1) | AU5334390A (en) |
| BR (1) | BR9006157A (en) |
| CA (1) | CA2029877A1 (en) |
| DD (1) | DD298338A5 (en) |
| DE (1) | DE3910061A1 (en) |
| HU (1) | HUT56694A (en) |
| PT (1) | PT93592A (en) |
| WO (1) | WO1990011013A1 (en) |
| ZA (1) | ZA902401B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1294811C (en) * | 2001-04-27 | 2007-01-17 | 味之素株式会社 | Growth promoters for gramineous plants |
| CN107683861A (en) * | 2016-08-05 | 2018-02-13 | 中国科学院微生物研究所 | A kind of composition for preventing and treating potato scab and preparation method thereof |
| CN109890205A (en) * | 2016-08-28 | 2019-06-14 | 以色列农业和农村发展部农业研究组织(范卡尼中心) | The method for controlling the fungal infection in plant |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3933964C1 (en) * | 1989-10-11 | 1991-04-11 | Btc Biotechnik International Gmbh, 2050 Hamburg, De | |
| GR1000953B (en) * | 1990-05-22 | 1993-03-16 | Btc Biotech Int | Fertilizing means and uses therefor |
| JP2613136B2 (en) * | 1991-05-14 | 1997-05-21 | 株式会社コスモ総合研究所 | Plant growth promoter |
| US6020288A (en) * | 1992-06-19 | 2000-02-01 | Nonomura; Arthur M. | Methods and compositions for enhancing cytochrome P450 in plants |
| EP0813510A4 (en) * | 1995-03-06 | 2000-07-26 | Arthur M Nonomura | Methods and compositions for enhancing formyltetrahydropteroylpolyglutamate in plants |
| JP2927269B2 (en) * | 1996-11-11 | 1999-07-28 | 味の素株式会社 | Plant root growth promoter |
| AU2001234685A1 (en) * | 2000-02-02 | 2001-08-14 | Andrew A. Benson | Methods and compositions for enhancing formyltetrahydropteroylpolyglutamate in plants |
| JP2001302427A (en) * | 2000-04-25 | 2001-10-31 | Nok Corp | Plant growth accelerator and method for accelerating growth of plant by using the accelerator |
| CN113711751B (en) * | 2021-09-07 | 2022-05-13 | 新洋丰农业科技股份有限公司 | Facility cultivation water and fertilizer integrated system and fertilizer application method |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DD63669A (en) * | ||||
| DE2625398A1 (en) * | 1976-06-05 | 1977-12-15 | Philips Patentverwaltung | Seed potato germination stimulation - with agents contg. cysteine, kinetin and calcium glycerophosphate and/or calcium pantothenate |
| JPS55500166A (en) * | 1978-03-28 | 1980-03-27 | Michael James Sampson | |
| ZA814783B (en) * | 1980-07-16 | 1982-07-28 | M Sampson | Disease control in plants |
| IT1196465B (en) * | 1986-07-07 | 1988-11-16 | Montedison Spa | FUNGICIDE MIXTURES |
| SU1446155A1 (en) * | 1987-04-02 | 1988-12-23 | Институт молекулярной биологии и биохимии АН КазССР | Method of producing regenerant plants of corn from tissue culture |
-
1989
- 1989-03-28 DE DE3910061A patent/DE3910061A1/en active Granted
-
1990
- 1990-03-28 DD DD90339159A patent/DD298338A5/en not_active IP Right Cessation
- 1990-03-28 EP EP90904830A patent/EP0422147A1/en not_active Withdrawn
- 1990-03-28 CN CN90104130A patent/CN1047191A/en active Pending
- 1990-03-28 JP JP2504931A patent/JPH03505585A/en active Pending
- 1990-03-28 ZA ZA902401A patent/ZA902401B/en unknown
- 1990-03-28 AU AU53343/90A patent/AU5334390A/en not_active Abandoned
- 1990-03-28 KR KR1019900702527A patent/KR920700002A/en not_active Application Discontinuation
- 1990-03-28 CA CA 2029877 patent/CA2029877A1/en not_active Abandoned
- 1990-03-28 PT PT93592A patent/PT93592A/en not_active Application Discontinuation
- 1990-03-28 HU HU902756A patent/HUT56694A/en unknown
- 1990-03-28 WO PCT/EP1990/000491 patent/WO1990011013A1/en not_active Application Discontinuation
- 1990-03-28 BR BR9006157A patent/BR9006157A/en not_active Application Discontinuation
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1294811C (en) * | 2001-04-27 | 2007-01-17 | 味之素株式会社 | Growth promoters for gramineous plants |
| CN107683861A (en) * | 2016-08-05 | 2018-02-13 | 中国科学院微生物研究所 | A kind of composition for preventing and treating potato scab and preparation method thereof |
| CN109890205A (en) * | 2016-08-28 | 2019-06-14 | 以色列农业和农村发展部农业研究组织(范卡尼中心) | The method for controlling the fungal infection in plant |
Also Published As
| Publication number | Publication date |
|---|---|
| EP0422147A1 (en) | 1991-04-17 |
| DE3910061A1 (en) | 1990-10-04 |
| DD298338A5 (en) | 1992-02-20 |
| JPH03505585A (en) | 1991-12-05 |
| ZA902401B (en) | 1991-01-30 |
| AU5334390A (en) | 1990-10-22 |
| KR920700002A (en) | 1992-02-19 |
| PT93592A (en) | 1990-11-07 |
| HUT56694A (en) | 1991-10-28 |
| BR9006157A (en) | 1991-08-06 |
| DE3910061C2 (en) | 1991-04-11 |
| CA2029877A1 (en) | 1990-09-29 |
| WO1990011013A1 (en) | 1990-10-04 |
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