JP2001302427A - Plant growth accelerator and method for accelerating growth of plant by using the accelerator - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a plant growth accelerator obtained by using a metabolic product of an industrially mass-culturable microbial strain as an active component. SOLUTION: The objective plant growth accelerator contains a spray-dried product of the metabolic product of Pseudomonas solanacearum (FERM P-15545) belonging to the genus Pseudomonas or Enterobacter cloacae (FERM P-8884) as an active component.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

[0001] The present invention relates to a plant growth promoting agent and a method for promoting plant growth using the same. More specifically, the present invention relates to a plant growth promoter containing a metabolite of a microorganism as an active ingredient and a method for promoting plant growth using the same.

[0002]

2. Description of the Related Art In order to promote the growth of plants, a method of spraying plant hormones or the like to plants is often used. The present applicant has previously clarified that a culture solution using a microorganism exhibits a plant growth promoting effect (Japanese Patent Publication No. 6-40).
No. 829). However, these microbial cultures have a problem that they are susceptible to other germs and do not lend themselves to long-term storage because a considerable amount of the medium component remains inside. As a countermeasure for this, it is conceivable to prepare immediately before use and use up all at once, but this is not a drastic solution. Furthermore, when these microbial cultures are sprayed, a considerable amount must be added to the tank at the time of watering, or a diluent must be separately prepared and sprayed on the plant, which is inconvenient to handle. .

As a solution to this problem, the present applicant has previously proposed to use a freeze-dried product of a microorganism culture solution as a plant growth promoter (Japanese Patent Application Laid-Open No. 2-240008). In addition, there is a problem that the operation is complicated, such as a long processing time and crushing after drying.

[0004]

SUMMARY OF THE INVENTION It is an object of the present invention to provide a plant growth promoting agent which uses a microorganism which can be industrially mass-cultured and has a metabolite thereof as an active ingredient.

[0005]

SUMMARY OF THE INVENTION The object of the present invention is as follows.
Psedomonas solanacea, a microorganism belonging to the genus Pseudomonas
rum (FERM P-15545) or Enterobacter cloacae (FERM
This is achieved by a plant growth promoter containing a spray-dried product of the metabolite of P-8884) as an active ingredient.

[0006]

BEST MODE FOR CARRYING OUT THE INVENTION The microorganism Psedom used in the present invention
onas solanacearum (FERM P-15545) and Enterobacter
Cloacae (FERM P-8888) has been discovered by the present inventors, and details thereof are described in JP-A-10-28593 and JP-B-6-40829, respectively. .

[0007] Psedomonas solanacearum and Enterobac
The culture of ter cloacae is carried out at 37 to 40 ° C. for about 72 to 90 hours using an arbitrary medium under shaking conditions. On this occasion,
For Psedomonas solanacearum, about 4
00-600 mg, preferably about 480-520 mg of adenine, Ent
When about 1 to 4 mg of tryptophan is added to 1 L of the medium for erobacter cloacae, the plant growth promoting effect of a spray-dried product of a metabolite obtained therefrom is further enhanced. Further, prior to the main culture, it is preferable to use a product pre-cultured in a similar medium.

The spray-dried product is cultured for a predetermined time, then centrifuged to collect the cells, and a culture solution is obtained as a metabolite.
The temperature in the chamber is about 160 to 190 ° C, preferably 180
° C, suction volume 40-50 m ³ / min, preferably 45 m ³ / min
It is obtained by spray drying under the conditions of 10 to 15 ml, preferably 12 ml / min, and outlet temperature of 70 to 90 ° C, preferably 80 ° C.

[0009] Plant cultivation using spray-dried materials
For example, it can be performed by the following method. (1) Spray-dried material is applied to seeds and then seeded on soil (2) Spray-dried material is dissolved in water and sprayed on plant leaves or drenched in soil (3) Spray-dried material Method of sowing seeds or planting seedlings in mixed soil
In the case of seeds, for example, seeds such as komatsuna, carnations, seedlings such as Phalaenopsis,
In the case of cloacae, for example, lettuce, cucumber, eggplant,
Seeds such as tomato, melon, cucumber, eggplant, tomato,
Seedlings such as rice are used.

[0010] If the culture solution itself is used instead of the spray-dried material, the seed treatment itself is difficult,
In addition, when seeds are treated with an aqueous solution, there are problems that the germination rate of the seeds is reduced and the seeds are easily rotted. Therefore, also in the case of nutrient cultivation, it is used after it is once spray-dried.

[0011]

Industrial Applicability The plant growth promoter of the present invention comprises a spray-dried product of a metabolite of a microorganism which can be industrially mass-cultured as an active ingredient. It has an effect that its preparation and handling are easy and long-term storage is possible.

[0012]

Next, the present invention will be described by way of examples. The present invention is not limited to these.

Example 1 Tryptone 1% Yeast extract 0.5% NaCl 0.5% Adenine 0.05% (final concentration) Distilled water Remainder 100 ml of a medium having the above composition was added to a 1 L Erlenmeyer flask, and the mixture was heated at 121 ° C, 1 atm. After sterilization under the condition of minutes, the mixture was cooled. And Psedomonas solanacearum (FERMP-15545)
Was added at 37 ° C. for 72 hours while shaking at 100 rpm, and then centrifuged (7000 rpm, 10 minutes, 4 ° C.) to collect the cells.

[0014] 200 ml of this culture solution was placed in a chamber at a temperature of 180
° C, suction amount 45m ³ / min, liquid sending amount 12ml / min, outlet temperature 80 ° C
Was spray-dried under the conditions described above to obtain about 2.5 g of a spray-dried product.

[0015] Komatsuna seeds were mixed into the spray-dried material, and the seeds were dusted with the spray-dried material. The soil was placed in a 5 L capacity planter, and 10 Komatsuna seeds or 10 Komatsuna seeds coated with the spray-dried material were sown and cultivated in a greenhouse for 30 days. When the maximum leaf length, the number of leaves and the dry weight (average of eight strains) of the grown Komatsuna were measured, the spray-dried ones were about 1.1 times larger than those from the Komatsuna seeds as shown in Table 1. Had the effect of promoting growth. Table 1 Number of leaves on untreated spray-dried material (sheets) 5.9 6.3 Maximum leaf length (cm) 26.8 29.0 Dry weight (g) 1.68 1.89

Example 2 (NH ₄ ) ₂ SO ₄ 0.1% KH ₂ PO ₄ 1.0% MgSO ₄ 0.01% Trisodium citrate 0.05% Glucose 0.4% Tryptophan 3 mg / ml (final concentration) 100 ml of a medium having the above composition was prepared. The solution was added to a 1-L Erlenmeyer flask, sterilized under the conditions of 121 ° C., 1 atm, and 15 minutes, and cooled. In addition, Enterobacter cloacae (FERM P-8844)
Was added at 37 ° C. for 72 hours while shaking at 100 rpm, and then centrifuged (7000 rpm, 10 minutes, 4 ° C.) to collect the cells.

Spray-drying was performed on 200 ml of the culture in the same manner as in Example 1 to obtain a spray-dried product.

Effect on Soil Cultivation Vermukilite is put into a 300 ml capacity death cup with a drain hole at the bottom, and lettuce seeds or lettuce seeds sprayed with spray-dried material are sown and grown in a greenhouse for 30 days. I let it. When the average weight of the 10 individuals on the aerial part of the grown lettuce was measured, the weight from the lettuce seeds was 41 g, while the weight of the spray-dried one was 62 g, about 1.5 g.
The growth promotion effect was doubled.

Effects on hydroponics (1) Lettuce seeds germinate and produce seedlings to a certain extent hydroponically cultivated (2) Lettuce seeds sprinkled with the above spray-dried material to germinate (3) Kumiai Hydroponic solution No. 1 and 1 liter of mixed solution of aqueous solution
Lettuce seeds grown in a solution to which 0.1 g of the above-mentioned spray-dried material has been added. (4) Dissolve the spray-dried material in water and spray 2 ml of the seedling on the first week of germination with 2 ml per seedling. The above-ground and root 16 after 30 days of growth under the above conditions
The average weight of the solid is shown in Table 2 below. Table 2 Conditions Aboveground part (g) Root (g) (1) 43.8 8.3 (2) 55.0 9.2 (3) 66.0 10.0 (4) 60.0 9.0

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Claims (6)

[Claims] 1. A microorganism Psedom belonging to the genus Pseudomonas
A plant growth promoter comprising a spray-dried product of a metabolite of onas solanacearum (FERM P-15546) as an active ingredient. 2. A microorganism Ente belonging to the genus Enterobacter.
A plant growth promoter comprising a spray-dried product of a metabolite of robacter cloacae (FERM P-8844) as an active ingredient. 3. A method for promoting plant growth, comprising applying the plant growth promoting agent according to claim 1 or 2 to a seed and then sowing the seed on soil. 4. A method for promoting plant growth, comprising dissolving the plant growth promoter according to claim 1 or 2 in water and spraying the solution on a leaf surface of a plant or irrigating the soil. 5. A method for promoting plant growth, comprising sowing seeds or planting seedlings in soil mixed with the plant growth promoting agent according to claim 1. 6. A method of promoting plant growth, comprising cultivating a plant using a fertilizer solution for hydroponics to which the plant growth promoter according to claim 1 or 2 is added.