CN111493095A - Plant growth regulator composition containing hypersensitive protein and application thereof - Google Patents
Plant growth regulator composition containing hypersensitive protein and application thereof Download PDFInfo
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- CN111493095A CN111493095A CN202010516560.8A CN202010516560A CN111493095A CN 111493095 A CN111493095 A CN 111493095A CN 202010516560 A CN202010516560 A CN 202010516560A CN 111493095 A CN111493095 A CN 111493095A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/12—Quaternary ammonium compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group, wherein Cn means a carbon skeleton not containing a ring; Thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof
- A01N37/30—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof containing the groups —CO—N< and, both being directly attached by their carbon atoms to the same carbon skeleton, e.g. H2N—NH—CO—C6H4—COOCH3; Thio-analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
- A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
- A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/24—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing the groups, or; Thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/36—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< directly attached to at least one heterocyclic ring; Thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
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- Dentistry (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
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Abstract
The invention relates to the technical field of pesticides, and particularly provides a plant growth regulating composition containing a hypersensitive protein and application thereof. In a first aspect, the present invention provides a plant growth regulating composition comprising a hypersensitive protein, said plant growth regulating composition comprising an active component a and an active component B; the active component A comprises Harpin protein, the active component B is selected from any one of s-abscisic acid, salicylic acid, pyraclostrobin, gibberellic acid, diethyl aminoethyl hexanoate, jasmonic acid, methyl jasmonate, phenmediphamine acid, forchlorfenuron, chlormequat chloride and choline chloride, the content of the Harpin protein is 0.01-30% by weight percent, and the weight ratio of the active component A to the active component B is 1: (0.1 to 50).
Description
Technical Field
The invention relates to the technical field of pesticides, and particularly provides a plant growth regulating composition containing a hypersensitive protein and application thereof.
Background
The plants are harmed by various diseases and insects in the growth process, and if the plants are not controlled, supplemented with nutrients and regulated to grow in time, the yield is reduced to different degrees, even the grains are not harvested, so that the nutrients are properly supplemented and the growth is regulated while the diseases and the insects are controlled, and the resistance of the plants is enhanced.
The Harpin protein is obtained by fermenting engineering bacteria containing natural Harpin protein genes, is easy to be identified by a plant surface receptor, can activate the conduction of endogenous signals in a plant body, and is a broad-spectrum plant function activator. The s-abscisic acid is a plant growth balance factor, is a natural abscisic acid, is a pure natural product contained in all green plants, is sensitive to light and belongs to a strong light decomposition compound. The s-abscisic acid is compounded by selecting plant growth regulators with different action mechanisms, so that the application range of the plant growth regulators can be enlarged, the action effect is improved, the dosage of the regulators is reduced, and the like.
The invention patent with application publication number CN104823975BA discloses a plant growth regulator, the effective components of which comprise 2- (acetoxyl) benzoic acid and s-abscisic acid, and the plant growth regulator is mainly used for regulating the growth and increasing the yield of rice and wheat in seedling stage, and has a small application range. In addition, the plant growth regulator widely used at present is a bactericide and a plant growth regulator which are prepared at present, the mixing proportion of the bactericide and the plant growth regulator has no scientific basis, the bactericide and the plant growth regulator are mixed together randomly, the synergist effect cannot be achieved, the antagonism effect can be achieved, the pesticide is wasted, and the deformity of leaves, fruits and the like of the plants and even the phytotoxicity are easily caused due to improper dosage. In addition, many plant growth regulators have problems such as inability to store for a long period of time and poor stability.
Disclosure of Invention
In order to solve the above technical problems, the present invention provides in a first aspect a plant growth regulating composition containing a hypersensitive protein, comprising an active component a and an active component B; the active component A comprises Harpin protein, the active component B is selected from any one of s-abscisic acid, salicylic acid, pyraclostrobin, gibberellic acid, diethyl aminoethyl hexanoate, jasmonic acid, methyl jasmonate, phenmediphamine acid, forchlorfenuron, chlormequat chloride and choline chloride, the content of the Harpin protein is 0.01-30% by weight percent, and the weight ratio of the active component A to the active component B is 1: (0.1 to 50).
According to a preferable technical scheme, the content of the Harpin protein is 1-10% by weight, and the weight ratio of the active component A to the active component B is 1: (0.1 to 20).
As a preferred technical scheme of the invention, the Harpin protein comprises a Harpin protein with higher homology with HarpinEccs protein.
As a preferred technical scheme of the invention, the Harpin protein also comprises Harpineccs fusion protein with Harpin functional activity and other Harpin fusion proteins which are obtained by a gene recombination mode.
In a preferred embodiment of the present invention, the plant growth regulating composition is prepared from an active ingredient and an adjuvant in a formulation acceptable to agricultural chemicals.
As a preferable technical scheme of the invention, the auxiliary agent is selected from one or a combination of a plurality of dispersing agents, wetting agents, antifreezing agents, thickening agents, emulsifying agents, defoaming agents, disintegrating agents, preservatives and fillers.
As a preferred technical scheme of the invention, the filler is selected from one or a combination of more of ammonium sulfate, sodium chloride, potassium chloride, starch, trehalose, glucose and urea; the weight ratio of the starch to the trehalose is (0.3-0.7): 1.
as a preferred embodiment of the present invention, the starch includes amylose and amylopectin; the weight ratio of the amylose to the amylopectin is 1: (0.3-0.5).
As a preferable technical scheme, the preparation form is any one of a suspending agent, an oil suspending agent, a microcapsule suspending agent, a suspoemulsion, an emulsion in water, a microemulsion, a wettable powder, a water dispersible granule, a water aqua, a soluble powder, a soluble tablet, a soluble granule, a soluble solution, a micro granule, a powder, a wettable granule and a seed coating agent.
The second aspect of the invention provides an application of the plant growth regulating composition containing the hypersensitive protein in regulating the growth of rape, apples, pears, broad beans, wheat, rice, cotton and corns.
Has the advantages that:
(1) the invention provides a plant growth regulating composition containing hypersensitive protein, which can get through the growth and development related factors of plants better by compounding specific active components, can obviously regulate and increase the growth of crops and promote fertilization, has obvious promoting effect on cell division, and has promoting effect on both transverse growth and longitudinal growth of organs, thereby playing the role of expanding fruits.
(2) The composition can effectively control the occurrence and spread of fruit diseases, can promote the differentiation of buds, promote the generation of lateral branches, improve the germination rate, promote the growth of roots and crowns of plants, promote the growth of roots and strong seedlings, protect flowers and fruits, greatly improve the yield of crops and improve the quality of the crops.
(3) The composition has excellent storage stability and durability through compounding of the active components and the auxiliary agent, can effectively play a role, is widely applied to vegetables, fruit trees and crops such as rape, apples, pears, broad beans, wheat, rice, cotton, corn and the like, has the advantages of remarkable apparent character of promoting plant growth, more lasting improvement on physiological function, quick response after use and remarkable yield increase effect, can better regulate the growth and development of crops, increases the resistance to various stresses, improves the yield and quality of the crops, and is a pesticide variety which is worthy of popularization and use.
Detailed Description
The disclosure may be understood more readily by reference to the following detailed description of preferred embodiments of the invention and the examples included therein. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In case of conflict, the present specification, including definitions, will control.
The terms "comprises," "comprising," "includes," "including," "has," "having," "contains," "containing," or any other variation thereof, as used herein, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.
When an amount, concentration, or other value or parameter is expressed as a range, preferred range, or as a range of upper preferable values and lower preferable values, this is to be understood as specifically disclosing all ranges formed from any pair of any upper range limit or preferred value and any lower range limit or preferred value, regardless of whether ranges are separately disclosed. For example, when a range of "1 to 5" is disclosed, the described range should be interpreted to include the ranges "1 to 4", "1 to 3", "1 to 2 and 4 to 5", "1 to 3 and 5", and the like. When a range of values is described herein, unless otherwise stated, the range is intended to include the endpoints thereof and all integers and fractions within the range.
In addition, the indefinite articles "a" and "an" preceding an element or component of the invention are not intended to limit the number requirement (i.e., the number of occurrences) of the element or component. Thus, "a" or "an" should be read to include one or at least one, and the singular form of an element or component also includes the plural unless the number clearly indicates the singular.
In order to solve the above technical problems, the present invention provides in a first aspect a plant growth regulating composition containing a hypersensitive protein, which comprises an active component a and an active component B; the active component A comprises Harpin protein, the active component B is selected from any one of s-abscisic acid, salicylic acid, pyraclostrobin, gibberellic acid, diethyl aminoethyl hexanoate, jasmonic acid, methyl jasmonate, phenmediphamine acid, forchlorfenuron, chlormequat chloride and choline chloride, the content of the Harpin protein is 0.01-30% by weight percent, and the weight ratio of the active component A to the active component B is 1: (0.1 to 50).
In a preferred embodiment, the content of the Harpin protein is 1-10% by weight, and the weight ratio of the active component A to the active component B is 1: (0.1 to 20).
Active component A
The active component A comprises Harpin protein.
The Harpin protein is a multifunctional protein encoded by a gram-negative plant pathogenic bacterium hrp (hypersensitive infection and pathophysiology gene), is rich in glycine (Gly), lacks cysteine (Cys), is stable under heat and is sensitive to protease.
In a preferred embodiment, the Harpin protein comprises a Harpin class protein with high homology to HarpinEccs proteins, such as HarpinEcc, HarpinEa, HarpinEch, Harpinpst, Harpinpsg, Harpinpss, and the like.
In a preferred embodiment, the Harpin protein further comprises HarpinEccs fusion protein and other Harpin fusion proteins with Harpin functional activity obtained by gene recombination, such as HarpinEccs + HarpinEcc, HarpinEccs + HarpinEa, HarpinEccs + HarpinEch, HarpinEcc + HarpinEca, HarpinEcc + HarpinEch, HarpinEa + HarpinEch, and the like.
The Harpin protein of the present invention is commercially available, including but not limited to, from the science and technology development ltd, agrogan, Hunan.
Active component B
The active component B is selected from any one of s-abscisic acid, salicylic acid, pyraclostrobin, gibberellic acid, diethyl aminoethyl hexanoate, jasmonic acid, methyl jasmonate, phenmediphamine acid, forchlorfenuron, chlormequat chloride and choline chloride.
In a more preferred embodiment, the active component B comprises s-abscisic acid.
The s-abscisic acid of the present invention can be obtained from commercially available sources, including but not limited to from Tetrapython python Tech technologies, Inc.
The applicant found that the system was added with active ingredient a comprising Harpin protein, active ingredient B comprising s-abscisic acid and the weight ratio of active ingredient a to active ingredient B was controlled to be 1: (0.1-50), a better plant growth promoting effect can be brought, probably because the Harpin protein can be used as an exciton and can be contacted with a cell surface receptor on a plant leaf or root system to send out a pathogen attack alarm, the activity of intracellular related enzyme and protein phosphorylation are activated through configuration change to form a second messenger, a signal is amplified, finally a plant is stimulated to generate a defense reaction through the regulation of a special gene, the damage of crops to diseases and various types of adverse environments can be stimulated from the inside to generate strong resistance and restoration capacity, the regeneration and recovery can be promoted quickly, and the damage of the crops caused by disasters can be greatly reduced or eliminated; the s-abscisic acid induces plants to enhance photosynthesis and absorb nutrient substances, promotes the transportation and accumulation of substances, can effectively control the occurrence and spread of diseases through the interaction of the s-abscisic acid and the nutrient substances, can better get through factors related to the growth and development of the plants, can obviously adjust and increase the growth of crops, and can greatly improve the yield of the crops and improve the quality of the crops.
Auxiliary agent
The anti-disease composition is prepared into a pesticide allowable dosage form by active ingredients and auxiliary agents; the formulation allowed on the pesticide is prepared by uniformly mixing and grinding the active ingredient and the auxiliary agent together to prepare the required formulation.
In a preferred embodiment, the auxiliary agent is selected from one or more of a dispersing agent, a wetting agent, an antifreeze agent, a thickening agent, an emulsifier, a defoaming agent, a disintegrating agent, a preservative and a filler.
In a more preferred embodiment, the dispersing agent is selected from one or more of sodium alginate, calcium lignosulfonate, sodium lignosulfonate, polyvinylpyrrolidone, sodium methylene bis-naphthalene sulfonate, naphthalene formaldehyde condensate sulfonate, polycarboxylic acid, polyoxyethylene polyoxypropylene ether block copolymer and fatty alcohol polyoxyethylene ether phosphate;
the wetting agent is selected from one or a combination of more of sodium dodecyl sulfate, sodium lauryl sulfate, nekal, sodium dodecyl benzene sulfonate, sodium diisobutyl sulfonate and fatty alcohol-polyoxyethylene ether;
the antifreeze is one or a combination of more of ethylene glycol, 1, 2-propylene glycol, glycerol, polyethylene glycol and sorbitol;
the thickening agent is selected from one or a combination of more of gum arabic, magnesium aluminum silicate, xanthan gum, polyvinyl alcohol, organic bentonite and white carbon black;
the emulsifier is selected from one or a combination of more of calcium dodecyl benzene sulfonate, styryl phenol polyoxyethylene ether phosphate, benzyl phenol polyoxyethylene ether phosphate, sorbitan trioleate, sorbitan tristearate, polyethylene glycol monostearate, glycerol monostearate, sorbitan monooleate, sorbitan monolaurate, polyoxyethylene lauryl ether, polyoxyethylene sorbitan monolaurate, polyoxyethylene monopalmitate, polyoxyethylene lauryl ether, sorbitan polyoxyethylene ether, polyoxyethylene sorbitan monooleate, styryl phenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, fatty amine polyoxyethylene ether and castor oil polyoxyethylene ether;
the defoaming agent is selected from one or a combination of a polyether defoaming agent, an organic silicon defoaming agent and a polyether modified polysiloxane defoaming agent;
the disintegrating agent is selected from one or more of sodium carboxymethyl starch, carboxypropyl cellulose and croscarmellose sodium;
the preservative is selected from one or a combination of more of kasong, formaldehyde, sodium benzoate, sorbic acid and potassium sorbate;
the filler is selected from one or a combination of more of ammonium sulfate, sodium chloride, potassium chloride, starch, trehalose, glucose and urea.
In a preferred embodiment, the formulation is any one of a suspension, an oil suspension, a microcapsule suspension, a suspoemulsion, an aqueous emulsion, a microemulsion, a wettable powder, a water dispersible granule, an aqueous solution, a soluble powder, a soluble tablet, a soluble granule, a soluble solution, a fine granule, a powder, a wettable granule and a seed coating.
Compositions for various dosage forms can be prepared using methods commonly used in the art. The compositions may be provided as a finished product in which the ingredients have been mixed together, or they may be provided as separate formulations in which the ingredients are mixed together immediately prior to use.
The contents of active ingredients and other ingredients in the above dosage forms have no special requirements, and the conventional proportion in the art can be adopted.
In a more preferred embodiment, the dosage form is any one of an aqueous agent, a soluble powder, a soluble tablet, a soluble granule, and a soluble solution.
In a more preferred embodiment, the dosage form is an aqueous or soluble powder.
In a more preferred embodiment, the dosage form is a soluble powder; the soluble powder comprises, by weight, 0.5-50% of active ingredients (an active component A and an active component B), 3-12% of a dispersing agent, 1-8% of a wetting agent, and the balance of a filler.
In a more preferred embodiment, the soluble powder comprises, in weight percent, 3% active ingredient a, 10% active ingredient B, 7% dispersing agent, 4% wetting agent, and the balance filler.
The soluble powder is prepared by uniformly mixing an active ingredient A, an active ingredient B, a dispersing agent, a wetting agent and a filler in a mixing cylinder, crushing the mixture by a jet mill and then uniformly mixing the crushed mixture.
In a more preferred embodiment, the filler comprises starch and trehalose.
In a more preferred embodiment, the weight ratio of starch to trehalose is (0.3-0.7): 1; more preferably, the weight ratio of starch to trehalose is 0.5: 1.
the applicant finds that the effect of promoting plant growth can be better brought by adding active components such as Harpin protein and s-abscisic acid in the system, but the effect of the obtained composition in various adverse environments such as high temperature and frost damage is weakened, and the effect of promoting plant growth can be more obvious and lasting by adding trehalose in the system. The trehalose can form a unique protective film under severe environmental conditions of high temperature, high cold, high osmotic pressure, drying and dehydration and the like, and has nonspecific protective action on biomacromolecules and organisms, so that active components are ensured to effectively break through relevant factors for plant growth and development, crop growth and fertilization can be obviously regulated and increased, and crop yield is greatly improved; the active ingredients in the prepared composition are easy to volatilize when the composition is diluted and placed at room temperature for a long time, the viscosity of the system can be increased when starch is added into the system, the diffusion and loss speed of small molecules can be slowed down, the storage time of the diluent is prolonged, and the trehalose carrying the active ingredients can quickly permeate into the inner layer of the plant, so that the composition can better act on plant cells and promote the growth and development of the plant. In addition, when the starch is excessively added, the growth promoting effect is weakened, and when the weight ratio of the starch to the trehalose is controlled to be (0.3-0.7): 1, the effect of promoting plant growth can be effectively improved, probably because when the starch is added too much, the viscosity of the system is easily increased, the uniformity of the system is reduced, and when the starch acts on the plant, the effective components are slowly released, so that the action effect is not ideal.
In a more preferred embodiment, the starch comprises amylose and amylopectin; the weight ratio of the amylose to the amylopectin is 1: (0.3 to 0.5); the weight ratio of the amylose to the amylopectin is 1: 0.4.
in a more preferred embodiment, the dispersing agent comprises a polycarboxylic acid and sodium alginate.
In a more preferred embodiment, the mesh number of the sodium alginate is 160-200; more preferably, the mesh number of the sodium alginate is 180.
In a more preferred embodiment, the weight ratio of the polycarboxylic acid to the sodium alginate is (1.1-1.3): 1; more preferably, the weight ratio of the polycarboxylic acid to the sodium alginate is 1.2: 1.
in a more preferred embodiment, the wetting agent is sodium lauryl sulfate.
The amylose and amylopectin of the present invention are purchased from Shanghai monogroup biology, Inc.; the trehalose is purchased from Shanghai Merlin Biotech, Inc.; the polycarboxylic acid is purchased from Japan bamboo oil and fat Co., Ltd, and the mark is YUS-WG 5; the sodium alginate was purchased from bio-technology development ltd of the forest of Qingdao sea.
The applicant finds that the added polycarboxylic acid and sodium alginate can improve the uniformity of a system, and particularly, the weight ratio of the polycarboxylic acid to the sodium alginate is 1: the amylose and the amylopectin of (0.3-0.5) can obviously improve the stability of effective components and the effect of promoting plant growth, probably because the polycarboxylic acid and the sodium alginate are easy to react with the coiled amylose and the branched amylopectin with shorter branches, so that the coiled spiral open carbon chain and the branched structure form a more stable space network structure in a system, the improvement of the dispersion effect is facilitated, the uniformity of the system is improved, and the effect is more obvious.
The applicant finds that when the sodium alginate is added excessively, the hydration rate is reduced and the dissolution effect is reduced when the sodium alginate is diluted for use, and when the mesh number of the sodium alginate is controlled to be 160-200 and the weight ratio of the polycarboxylic acid to the sodium alginate is controlled to be (1.1-1.3): 1, the dispersion of the system is better, and the plant growth is promoted. The reason is probably that after the sodium alginate with the mesh number of 160-200 becomes wet with water, particles with proper size are formed, and hydration and dissolution are facilitated; in addition, sodium alginate is also favorably adsorbed on the surface of plant cells to promote the growth of plant bodies; when the polycarboxylic acid is added too much, the hydrophilicity of the sodium alginate is reduced, the molecular chain is contracted, and when the weight ratio of the polycarboxylic acid to the sodium alginate is controlled to be (1.1-1.3): 1, they cooperate with each other to achieve an ideal overall dispersibility.
The disease or pest resistant composition of the present invention may contain, in addition to the above-mentioned essential components, a protein protecting agent and a stabilizer as appropriate within a range not to impair the effects of the present invention.
Examples of such components include polyhydroxy compounds, sugars, amino acids, and the like, but are not limited to these.
Examples of the polyhydric compound include glycerin, mannitol, sorbitol, inositol, thiol, polyethylene glycol, etc., examples of the sugar include sucrose, lactose, cellobiose, mannose, maltose, inositol, raffinose, inulin, dextran, maltodextrin, maltopolysaccharide, sucrose octasulfate, heparin, 2-hydroxypropyl- β cyclodextrin, etc., and examples of the amino acid include proline, L-tryptophan, sodium glutamate, alanine, glycine, lysine hydrochloride, sarcosine, L-tyrosine, phenylalanine, arginine, etc.
In a second aspect, the invention provides the use of a plant growth regulating composition containing a hypersensitive protein for regulating the growth of rape, apples, pears, broad beans, wheat, rice, cotton and corn.
The plant growth regulating composition can be applied by adding water according to a common method, the amount of the added water can be automatically regulated and controlled according to requirements, and the plant growth regulating composition can be used for spray stem leaf treatment, soil treatment, such as solid root broadcasting or liquid root irrigation, and seed dressing, seed soaking or seed coating. The dosage of the plant growth regulating composition in each acre of land is not particularly limited, and can be specifically regulated according to the acre dosage of the active ingredients and the content of the active ingredients in the preparation.
Examples
In order to better understand the above technical solutions, the following detailed descriptions will be provided with reference to specific embodiments. It should be noted that the following examples are only for illustrating the present invention and should not be construed as limiting the scope of the present invention, and that the insubstantial modifications and adaptations of the present invention by those skilled in the art based on the above disclosure are still within the scope of the present invention. In addition, the starting materials used are all commercially available, unless otherwise specified.
Example 1
Example 1 of the present invention provides a plant growth regulating composition containing a hypersensitive protein, which comprises an active component a and an active component B; the active component A comprises Harpin protein, the active component B comprises s-abscisic acid, and the content of the Harpin protein is 3% and the content of the s-abscisic acid is 10% in percentage by weight.
The plant growth regulating composition is prepared into a pesticide allowable dosage form by active ingredients and auxiliary agents.
The dosage form is soluble powder; the soluble powder also contains 7 percent of dispersing agent, 4 percent of wetting agent and the balance of filling material according to weight percentage.
Mixing the active component A, the active component B, the dispersing agent, the wetting agent and the filler together in a mixing cylinder uniformly, crushing the mixture by a jet mill, and then mixing the mixture uniformly to prepare the soluble powder.
The filler comprises starch and trehalose.
The weight ratio of the starch to the trehalose is 0.5: 1.
the starch comprises amylose and amylopectin; the weight ratio of the amylose to the amylopectin is 1: 0.4.
the dispersing agent comprises polycarboxylic acid and sodium alginate; the mesh number of the sodium alginate is 180.
The weight ratio of the polycarboxylic acid to the sodium alginate is 1.2: 1.
the wetting agent is sodium dodecyl sulfate.
The Harpin protein is purchased from science and technology development Co., Ltd of agricultural Dage of Hunan; the s-abscisic acid is purchased from the Tech technologies, Inc. of Szechwan python; the amylose and amylopectin are purchased from Shanghai monogroup biology, Inc.; the trehalose is purchased from Shanghai Merlin Biotech, Inc.; the polycarboxylic acid is purchased from Japan bamboo oil and fat Co., Ltd, and the mark is YUS-WG 5; the sodium alginate was purchased from bio-technology development ltd of the forest of Qingdao sea.
Example 2
Example 2 of the present invention provides a plant growth regulating composition containing a hypersensitive protein, which is specifically illustrated in example 1, except that the weight ratio of starch to trehalose is replaced with 2: 1.
example 3
Example 3 of the present invention provides a plant growth regulating composition containing a hypersensitive protein, which is embodied in the same manner as example 1, except that starch is not present.
Example 4
Example 4 of the present invention provides a plant growth regulating composition containing a hypersensitive protein, which is specifically illustrated in example 1, except that the weight ratio of amylose to amylopectin is replaced with 1: 2.
example 5
Example 5 of the present invention provides a plant growth regulating composition containing a hypersensitive protein, which is embodied in the same manner as example 1 except that amylopectin is not included.
Example 6
Example 6 of the present invention provides a plant growth regulating composition containing a hypersensitive protein, which is embodied in the same manner as example 1 except that amylose is not included.
Example 7
Example 7 of the present invention provides a plant growth regulating composition containing a hypersensitive protein, which is embodied in the same manner as example 1 except that the mesh number of sodium alginate is 60 instead, which is purchased from bio-technology development ltd of forest, Qingdao Hai.
Example 8
Example 8 of the present invention provides a plant growth regulating composition containing a hypersensitive protein, which is embodied in the same manner as example 1 except that the mesh number of sodium alginate is 240, which is purchased from bioscience development ltd of forest, Qingdao Hai.
Example 9
Example 9 of the present invention provides a plant growth regulating composition containing a hypersensitive protein, which is the same as example 1 in specific embodiment except that the weight ratio of polycarboxylic acid to sodium alginate is replaced with 1.2: 2.
example 10
Example 10 of the present invention provides a plant growth regulating composition containing a hypersensitive protein, which is embodied in the same manner as example 1, except that sodium alginate is not included.
Example 11
Example 11 of the present invention provides a plant growth regulating composition containing a hypersensitive protein, which is embodied in the same manner as example 1, except that polycarboxylic acid is not included.
Performance testing
1. Storage durability test
After the composition prepared in examples 1 to 11 was prepared into a 500-fold diluted solution and placed in a dust-free open air at room temperature for one week, the mass loss of the composition was calculated and recorded as w, and the calculation formula is as follows:
wherein m is0Mass of the original composition, m1Is the mass of the composition after one week of standing.
2. System stability test
The compositions prepared in the embodiments 1-11 are prepared into 500 times of diluent, whether layering and flocculation phenomena exist during dilution is observed, 100 parallel samples are respectively arranged in each embodiment, and the layering and flocculation probabilities, namely the percentage of the number of layering and flocculation in the total number of the tested compositions, are recorded.
TABLE 1
| Storage durability (%) | Stability of System (%) | |
| Example 1 | 2 | 0 |
| Example 2 | 5 | 11 |
| Example 3 | 19 | 3 |
| Example 4 | 5 | 9 |
| Example 5 | 8 | 4 |
| Example 6 | 5 | 10 |
| Example 7 | 5 | 8 |
| Example 8 | 4 | 6 |
| Example 9 | 4 | 7 |
| Example 10 | 9 | 3 |
| Example 11 | 14 | 16 |
3. Test of field efficacy of broad beans
Test site: shanxi province Hanzhong city Hantai Wuxiang town Wanjiawan village
The basic conditions of the test field are that the soil quality sandy soil, the soil species gray alluvial soil, the pH value is 7.1, the sweet potatoes are planted before, the large area is 10.7 meters long, 9.5 meters wide, the area is 101.65 square meters, the area is 0.1525 mu, the sowing period is 10 months and 29 days, the pits are dug and the dibbling is carried out, the specification is 60 × 48cm, the pit is 2083 pits per mu, 2-3 seeds are sowed in each pit, and the variety is broad beans with large white color.
The test method comprises the following steps: the plant growth regulating composition containing the hypersensitive protein prepared in the example 1 is prepared into 500-fold diluent as a test group, a control group is arranged, and the specific treatment process comprises the following steps:
CK: no treatment is carried out; treatment with the composition of the invention: spraying 3 times in 12 months, 16 days, 1 month, 12 days, 2 months and 4 days. Other field management measures are the same.
The growth stimulating effect for the different treatments is shown in table 2.
TABLE 2
4. Test of field efficacy of bergamot pear
Test site: korla awatt farm
Basic conditions of the test field: the planting area is 5 mu, and the test area is 1.5 mu. The soil is pure black soil. The bergamot pear is Korla bergamot pear, and the tree age is five years.
The test method comprises the following steps: the plant growth regulating composition containing the hypersensitive protein prepared in the example 1 is prepared into 500-fold diluent as a test group, a control group is arranged, and the specific treatment process comprises the following steps: CK: no treatment is carried out; treatment with the composition of the invention: spraying 4 times in 21 days 4 months, 21 days 5 months, 15 days 6 months and 17 days 7 months.
During the test period, the growth conditions of the bergamot pears in the test group and the control group are carefully observed, and the yield is measured before harvesting. Survey of production and sampling: taking three points of a test group and a control group respectively, selecting 3 plants at each point, and measuring the fruit bearing number and the fruit weight of a single plant of the bergamot pear; and calculating the number of plants per mu of the bergamot pears, and respectively calculating the yields of the bergamot pears treated and compared according to the average data. The growth stimulating effect for the different treatments is shown in table 3.
TABLE 3
The foregoing examples are merely illustrative and serve to explain some of the features of the method of the present invention. The appended claims are intended to claim as broad a scope as is contemplated, and the examples presented herein are merely illustrative of selected implementations in accordance with all possible combinations of examples. Accordingly, it is applicants' intention that the appended claims are not to be limited by the choice of examples illustrating features of the invention. Also, where numerical ranges are used in the claims, subranges therein are included, and variations in these ranges are also to be construed as possible being covered by the appended claims.
Claims (10)
1. A plant growth regulating composition containing a hypersensitive protein, wherein the plant growth regulating composition comprises an active component a and an active component B; the active component A comprises Harpin protein, the active component B is selected from any one of s-abscisic acid, salicylic acid, pyraclostrobin, gibberellic acid, diethyl aminoethyl hexanoate, jasmonic acid, methyl jasmonate, phenmediphamine acid, forchlorfenuron, chlormequat chloride and choline chloride, the content of the Harpin protein is 0.01-30% by weight percent, and the weight ratio of the active component A to the active component B is 1: (0.1 to 50).
2. The plant growth regulating composition containing the hypersensitive protein according to claim 1, wherein the Harpin protein is contained in an amount of 1-10% by weight, and the weight ratio of the active component a to the active component B is 1: (0.1 to 20).
3. The hypersensitive protein-containing plant growth regulating composition according to claim 1, wherein said Harpin protein comprises a Harpin-like protein having higher homology to HarpinEccs protein.
4. The plant growth regulating composition containing the hypersensitive protein according to claim 1 or 3, wherein the Harpin protein further comprises HarpinEccs fusion protein and other Harpin fusion proteins having Harpin functional activity obtained by means of gene recombination.
5. The plant growth regulating composition containing the hypersensitive protein according to claim 1, wherein the plant growth regulating composition is prepared into a pesticide-allowed formulation from an active ingredient and an adjuvant.
6. The plant growth regulating composition containing hypersensitive protein according to claim 5, wherein said adjuvant is selected from one or more of dispersing agent, wetting agent, antifreezing agent, thickening agent, emulsifying agent, antifoaming agent, disintegrating agent, preservative, and filler.
7. The plant growth regulating composition containing hypersensitive protein according to claim 6, wherein said filler is selected from the group consisting of ammonium sulfate, sodium chloride, potassium chloride, starch, trehalose, glucose and urea; the weight ratio of the starch to the trehalose is (0.3-0.7): 1.
8. the plant growth regulating composition containing the hypersensitive protein according to claim 7, wherein said starch comprises amylose and amylopectin; the weight ratio of the amylose to the amylopectin is 1: (0.3-0.5).
9. The hypersensitive protein-containing plant growth regulating composition according to claim 5, wherein the formulation is any one of a suspension, an oil suspension, a microcapsule suspension, a suspoemulsion, an aqueous emulsion, a microemulsion, a wettable powder, a water dispersible granule, an aqueous solution, a soluble powder, a soluble tablet, a soluble granule, a soluble solution, a microgranule, a powder, a wettable granule, and a seed coating.
10. Use of a plant growth regulating composition containing a hypersensitive protein according to any one of claims 1 to 9 for regulating the growth of rape, apples, pears, broad beans, wheat, rice, cotton and corn.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115486454A (en) * | 2021-11-04 | 2022-12-20 | 芮城华农生物化学有限公司 | Protein plant function regulating composition and application thereof |
| CN116035016A (en) * | 2022-10-18 | 2023-05-02 | 山东科赛基农生物工程有限公司 | High-efficiency weeding composition containing fenpyrazamine, and preparation method and application thereof |
-
2020
- 2020-06-09 CN CN202010516560.8A patent/CN111493095A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115486454A (en) * | 2021-11-04 | 2022-12-20 | 芮城华农生物化学有限公司 | Protein plant function regulating composition and application thereof |
| CN116035016A (en) * | 2022-10-18 | 2023-05-02 | 山东科赛基农生物工程有限公司 | High-efficiency weeding composition containing fenpyrazamine, and preparation method and application thereof |
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Inventor after: Tian Minghan Inventor after: Yang Jinhui Inventor after: Li Yuanjian Inventor after: Tian Bao Inventor after: Liu Xingzhi Inventor after: Yang Zhenjun Inventor before: Tian Minghan Inventor before: Yang Jinhui Inventor before: Li Yuanjian Inventor before: Tian Bao Inventor before: Liu Xingzhi Inventor before: Yang Zhenjun |
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Application publication date: 20200807 |