CN104718954A - Radix pseudostellariae cutting propagation method - Google Patents
Radix pseudostellariae cutting propagation method Download PDFInfo
- Publication number
- CN104718954A CN104718954A CN201510153737.1A CN201510153737A CN104718954A CN 104718954 A CN104718954 A CN 104718954A CN 201510153737 A CN201510153737 A CN 201510153737A CN 104718954 A CN104718954 A CN 104718954A
- Authority
- CN
- China
- Prior art keywords
- cuttings
- bactericide
- root
- cuttage
- branch
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
A radix pseudostellariae cutting propagation method is characterized by comprising the following steps that 1, ear picking is carried out, wherein semi-lignified stem vine branches on a mother seedling are selected, and are cut off from the rootstock of a stem vine; 2, sterilization is carried out, wherein the cut semi-lignified stem vine branches are placed in bactericide to be soaked for eight to ten minutes for sterilization; 3, ear cutting is carried out; 4, rooting agent and bactericide treatment is carried out; 5, seedbed treatment is carried out, wherein sandy soil is selected as seedbed media, impurities in the seedbed media are removed, soil loosening and leveling are carried out, and the seedbed media are watered through the bactericide for sterilization; 6, cuttage is carried out; 7, cutting management is carried out.
Description
Technical field
The present invention relates to technical field of cultivation, be specifically related to a kind of radix pseudostellariae cuttage breeding method.
Background technology
Radix pseudostellariae, has another name called caryophyllaceous ginseng, Tong Can.For the block root of Caryophyllaceae herbaceos perennial pseudostellaria heterophylla, be distributed widely in the ground such as East China, Central China, North China, northeast and northwest, medicinal material main product in Jiangsu, Shandong, the ground such as Anhui also produce, and are determined to list in " the traditional Chinese medicine list that can be used for health food " by the Ministry of Public Health at present.
Summary of the invention
The technical problem to be solved in the present invention is: the invention provides a kind of radix pseudostellariae cuttage breeding method, solves the survival rate existing for method of forestation in the past and the lower problem of storage rate.The technical scheme of technical solution problem of the present invention is:
(1) fringe is adopted: select the climing branch of semi-lignified stem on female seedling, from the rhizome position clip that stem is climing;
(2) sterilization processing: the climing branch of semi-lignified stem of clip is put into bactericide and soaks 8-10 minute sterilization processing;
(3) fringe is cut: on the climing branch of semi-lignified stem, choose the leaf that will gather, respectively respectively partial application is cut to branch in the petiole base attachment region both sides of leaf, namely respectively respectively partial application is cut to branch at leaf from 0.5-1cm and the above 4-6cm of attachment region below petiole base attachment region, otch is concordant, is just cut from the climing branch of stem together with the branch of described shearing by the leaf with blade face and petiole; And 2/3-1/2 area is wiped out by end of blade to the direction of phyllopodium in blade face, make the cuttings that half leaf one saves;
(4) root-growing agent, bactericide process: cuttings petiole base attachment region is immersed in root-growing agent with lower part and soaks, after having soaked, cuttings is immersed in bactericide completely and soaks;
(5) seedbed process: select sand as medium of seedling bed, the impurity removed in medium of seedling bed also loosens the soil smooth, then adopts bactericide to water medium of seedling bed to carry out disinfection sterilization;
(6) cuttage: every bar cuttings petiole base comprises petiole base with lower part and inserts medium of seedling bed, cuttage seeding goes out from nursery behind garden takes following measures to transplanting into the process of land for growing field crops: for in-plant transplanting situation: lonicera japonica nursery stock enters land for growing field crops being unearthed from nursery lifting to transplanting, if the time is in 48 hours, then in transportation, seedling root is dipped in thin mud, for transplanting situation at a distance: lonicera japonica nursery stock enters land for growing field crops being unearthed from nursery lifting to transplanting, if the time was more than 48 hours, or carry out temporary reservoir, then seedling root is dipped clay, the moisturizing mud of water-loss reducer and water mixing, the configuration proportion of moisturizing mud is: particulate water-loss reducer: clay: water=1: 120-160: 180-220, aforementioned proportion is volume ratio,
(7) cuttings management: after cuttings cuttage completes, bactericide is directly watered and be filled in cuttings, build transparent membrane shed sealing cuttings surrounding space again, therebetween without the need to Water spray, bear after sprouting until cuttings and remove transparent membrane shed, allow the cuttings growing sprouting grow in the sun, Routine Management is moved to field production after several moon.
The climing branch of semi-lignified stem described in described step (1) is that branch is not with spot, toughness is large, diameter is 1-2.5 millimeter the climing branch of stem.
In described step (4), first configure root-growing agent solution, then the cuttings saved by half leaf one soaks 40-50 minute in petiole base attachment region is with lower part immersion root-growing agent solution, whole immersion process middle period face portion does not contact with root-growing agent solution; After immersion completes, cuttings is taken out from root-growing agent solution and cuttings is immersed in bactericide completely soak after 15-25 minute and take out, prepare to be used for cuttage, described root-growing agent for adding indolebutyric acid 50-100 milligram in every premium on currency, 5% aqua jinggangmycin 15-25 gram, alcohol-soluble CPPU 0.1-0.4 milligram make; The bactericide adopted is carbendazim.
In described step (5), described medium of seedling bed silt content is 50-90%, and cuttage prefinishing seedbed is removed various impurity and loosened the soil smooth; Directly water with the microbicide solution that 50% wettable powder dilution agent 200 of carbendazim is doubly made again and be filled on medium of seedling bed.
In described step (7), the bactericide of employing is carbendazim, and described transparent membrane shed adopts bamboo chip and film paper to make, and builds sealing cuttings surrounding space along seedbed.
Radix pseudostellariae cuttage breeding method of the present invention, cutting medium, without the need to selecting thin river sand, dragon's blood sand or the perlite etc. through being exposed to the sun, only need select to carry out in general sand, and cutting medium need not make especially, low to the requirement of cutting medium, cost of manufacture is corresponding reduction also.On the other hand, play during cuttings sends adventive root when from cuttings cuttage to matrix, the encapsulation process of transparent membrane shed is adopted to keep the humidity of cuttings surrounding environment, instead of in the past loaded down with trivial details water mist sprinkling work, thus also making management work become easy, management cost also reduces greatly.
Embodiment
Below in conjunction with specific embodiment, the present invention is described further.
Embodiment 1
In nursery, select the climing branch of stem that leaf color is dark green, be in semi-lignified degree, by it from the climing rhizome position clip of stem, concentrate to put well to be ready for use on and cut fringe.The climing branch of semi-lignified stem is that branch is not with spot, toughness is large, diameter is 1-2.5 millimeter the climing branch of stem.When adopting ear selection and selecting, can in above-mentioned diameter range.
Sterilization processing
The climing branch of semi-lignified stem of clip is put into the process of bactericide soaking sterilization.
First configuring bactericide: bactericide is to kill suppression thalli growth, avoiding cuttings to be subject to bacteriological infection and withered rotten, effectively improving the survival rate of cuttage.The kind of bactericide is a lot, can select: jinggangmeisu, topsin, tpn, benomyl, streptomycin etc., the preferred jinggangmeisu of the present embodiment (producer: Tonglu, Zhejiang Province biochemical industry Co., Ltd), jinggangmeisu is the disinfectant use in agriculture that systemic action is very strong, uses the microbicide solution that jinggangmeisu 5% aqua compound concentration is 50g/L.
Sterilization: climing for the stem gathered in nursery branch is immersed in the above-mentioned microbicide solution prepared completely soak 8-10 minute again, to disturb and to suppress somatic cells g and D.Soak time any numerical value within the scope of 8-10 minute can realize the effect of bactericidal completely.
Cut fringe
On the climing branch of stem after sterilization processing, choose the leaf that will gather, respectively respectively partial application is cut to branch in the petiole base attachment region both sides of leaf, namely below petiole base attachment region, 0.5-1cm and above 5cm cuts partial application respectively, otch is concordant, is just cut from the climing branch of stem by the leaf with blade face and petiole.And then a part is wiped out by end of blade to the direction of phyllopodium in blade face, be generally wipe out blade face 2/3-1/2 area, make half leaf one and save cuttings.The cuttings finally saved by half good for clip leaf one is bundled into a bundle by the quantity rubber band of about 30 at the above 2-4cm place of cuttings petiole base attachment region, and keeps bottom concordant to facilitate immersion treatment.
The supply mainly carrying out moisture in perfect aspect by root system due to plant corpus supplements, and cuttings is not before root system grows, cuttings could not obtain supplementing of moisture, therefore cuttings blade face part must be wiped out 2/3-1/2 area to reduce the moisture transpiration of cuttings itself, makes cuttings not easily withered and inactivation.
First configure root-growing agent solution, then the cuttings saved by half leaf one soaks 40-50 minute in petiole base attachment region is with lower part immersion root-growing agent solution, whole immersion process middle period face portion does not contact with root-growing agent solution; After immersion completes, cuttings is taken out from root-growing agent solution and cuttings is immersed in completely in 50% wetting powder, 200 times of carbendazim germicidal solutions to soak after 15-25 minute and take out, prepare to be used for cuttage.Root-growing agent for adding alcohol-soluble CPPU 0.1-0.4 milligram in every premium on currency, indolebutyric acid 50-100 milligram, 5% aqua jinggangmycin 15-25 restrain into; The bactericide adopted is carbendazim, namely adopts 50% wettable powder dilution agent 200 of carbendazim doubly to make Bavistin solution.
Following pulp furnish can be adopted in the present embodiment:
| Raw material | Proportioning 1 | Proportioning 2 | Proportioning 3 | Proportioning 4 | Proportioning 5 |
| Alcohol-soluble CPPU | 0.1mg | 0.2mg | 0.3mg | 0.4mg | 0.25mg |
| Indolebutyric acid | 60mg | 80mg | 75mg | 70mg | 100mg |
| 5% aqua jinggangmycin | 16g | 20g | 18g | 25g | 20g |
CPPU is called the basic element of cell division, has acceleration cell mitogen, promotes the effect that cell increases and breaks up.Indolebutyric acid can promote the rooting of cuttings of plant, especially fairly obvious to rooting effect.Jinggangmycin has stronger interior absorption, is easily absorbed by somatic cells and within it conducts rapidly, and interference and suppression somatic cells g and D, effectively ensure that cuttings improves cuttage survival rate in period of growing from bacteriological infection.If do not carry out root-growing agent process, the survival rate of whole cuttage will be very low.
Seedbed processes
Cuttage seedbed is selected at sand as medium of seedling bed, and the partially middle high level of its silt content, any number can within the scope of 50-90% for silt content.Before cuttage, seedbed is organized into width 85cm, length is not limit, and medium of seedling bed is removed the impurity such as plastic sack, leaf branch and loosened the soil smooth.Directly watering with bactericide is filled in disinfection on medium of seedling bed again, through the seedbed of above process, just can be used for cottage propagation.Adopt the object in bactericide pouring seedbed be due in soil containing a large amount of bacterium, cuttings is vulnerable to bacteriological infection and affects and survive, can effective bacteria growing inhibiting through the seedbed of sterilization processing, makes cuttings from bacteriological infection.The kind of bactericide is a lot, can select: jinggangmeisu, topsin, tpn, benomyl, streptomycin etc., and the present embodiment adopts carbendazim, and the 50% wettable powder dilution agent 200 by carbendazim doubly makes microbicide solution.
Cuttage
Untie the cuttings bundled, every bar cuttings petiole base is comprised petiole base itself with lower part and inserts in medium of seedling bed, the distance between cuttings is not overlapping between leaf being advisable.
Cuttings manages
When cuttings cuttage completes, and then bactericide directly waters and is filled in cuttings, and pouring bactericide is that the space of cuttings petiole base covers by soil after pouring on the one hand, and bactericide can suppress seedbed bacterial growth to improve the survival rate of cuttage on the other hand.Then build transparent membrane shed by bamboo chip and film paper along seedbed, sealing cuttings surrounding space, need not spray therebetween.With film paper 13-15 consecutive days of sealing, the petiole joint eye place spot-check just visible cuttings grows adventive root, continue shed to be sealed to 20-25 consecutive days visible cuttings grown sprouting, now just can raise the film paper of sealing, allow the cuttings growing sprouting grow in the sun, and the seedling fostering management by methods time of 5 months is just used for field production in extensible nursery routinely.The kind of bactericide is a lot, can adopt jinggangmeisu, topsin, tpn, benomyl, streptomycin etc., and the present embodiment adopts carbendazim, and the 50% wettable powder dilution agent 400 by carbendazim doubly makes Bavistin solution.
Experiment: the present invention selects 2m respectively
2, 4m
2, 10m
2seedbed carry out cuttage experiment.Wherein the silt content in three seedbeds is respectively 50%, 70%, 90%, and cuttage survival rate is respectively 78%, 82%, 75%, average survival nearly 80%, and every mu can output seedling about 200,000 strain.
Claims (5)
1. a radix pseudostellariae cuttage breeding method, is characterized in that, comprises the following steps:
(1) fringe is adopted: select the climing branch of semi-lignified stem on female seedling, from the rhizome position clip that stem is climing;
(2) sterilization processing: the climing branch of semi-lignified stem of clip is put into bactericide and soaks 8-10 minute sterilization processing;
(3) fringe is cut: on the climing branch of semi-lignified stem, choose the leaf that will gather, respectively respectively partial application is cut to branch in the petiole base attachment region both sides of leaf, namely respectively respectively partial application is cut to branch at leaf from 0.5-1cm and the above 4-6cm of attachment region below petiole base attachment region, otch is concordant, is just cut from the climing branch of stem together with the branch of described shearing by the leaf with blade face and petiole; And 2/3-1/2 area is wiped out by end of blade to the direction of phyllopodium in blade face, make the cuttings that half leaf one saves;
(4) root-growing agent, bactericide process: cuttings petiole base attachment region is immersed in root-growing agent with lower part and soaks, after having soaked, cuttings is immersed in bactericide completely and soaks;
(5) seedbed process: select sand as medium of seedling bed, the impurity removed in medium of seedling bed also loosens the soil smooth, then adopts bactericide to water medium of seedling bed to carry out disinfection sterilization;
(6) cuttage: every bar cuttings petiole base comprises petiole base with lower part and inserts medium of seedling bed, cuttage seeding goes out from nursery behind garden takes following measures to transplanting into the process of land for growing field crops: for in-plant transplanting situation: lonicera japonica nursery stock enters land for growing field crops being unearthed from nursery lifting to transplanting, if the time is in 48 hours, then in transportation, seedling root is dipped in thin mud, for transplanting situation at a distance: lonicera japonica nursery stock enters land for growing field crops being unearthed from nursery lifting to transplanting, if the time was more than 48 hours, or carry out temporary reservoir, then seedling root is dipped clay, the moisturizing mud of water-loss reducer and water mixing, the configuration proportion of moisturizing mud is: particulate water-loss reducer: clay: water=1: 120-160: 180-220, aforementioned proportion is volume ratio,
(7) cuttings management: after cuttings cuttage completes, bactericide is directly watered and be filled in cuttings, build transparent membrane shed sealing cuttings surrounding space again, therebetween without the need to Water spray, bear after sprouting until cuttings and remove transparent membrane shed, allow the cuttings growing sprouting grow in the sun, Routine Management is moved to field production after several moon.
2. radix pseudostellariae cuttage breeding method according to claim 1, is characterized in that, the climing branch of semi-lignified stem described in described step (1) is that branch is not with spot, toughness is large, diameter is 1-2.5 millimeter the climing branch of stem.
3. radix pseudostellariae cuttage breeding method according to claim 1, it is characterized in that, in described step (4), first configure root-growing agent solution, the cuttings saved by half leaf one again soaks 40-50 minute in petiole base attachment region is with lower part immersion root-growing agent solution, and whole immersion process middle period face portion does not contact with root-growing agent solution; After immersion completes, cuttings is taken out from root-growing agent solution and cuttings is immersed in bactericide completely soak after 15-25 minute and take out, prepare to be used for cuttage, described root-growing agent for adding indolebutyric acid 50-100 milligram in every premium on currency, 5% aqua jinggangmycin 15-25 gram, alcohol-soluble CPPU 0.1-0.4 milligram make; The bactericide adopted is carbendazim.
4. a kind of radix pseudostellariae cuttage breeding method according to claim 1, is characterized in that, in described step (5), described medium of seedling bed silt content is 50-90%, and cuttage prefinishing seedbed is removed various impurity and loosened the soil smooth; Directly water with the microbicide solution that 50% wettable powder dilution agent 200 of carbendazim is doubly made again and be filled on medium of seedling bed.
5. radix pseudostellariae cuttage breeding method according to claim 1, it is characterized in that, in described step (7), the bactericide of employing is carbendazim, described transparent membrane shed adopts bamboo chip and film paper to make, and build sealing cuttings surrounding space along seedbed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510153737.1A CN104718954A (en) | 2015-04-02 | 2015-04-02 | Radix pseudostellariae cutting propagation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510153737.1A CN104718954A (en) | 2015-04-02 | 2015-04-02 | Radix pseudostellariae cutting propagation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104718954A true CN104718954A (en) | 2015-06-24 |
Family
ID=53443829
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510153737.1A Pending CN104718954A (en) | 2015-04-02 | 2015-04-02 | Radix pseudostellariae cutting propagation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104718954A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102640630A (en) * | 2011-02-21 | 2012-08-22 | 北京灏銮科技发展有限公司 | Transplanting survival keeping method for honeysuckle |
| CN102640629A (en) * | 2011-02-21 | 2012-08-22 | 深圳市中科时代生物科技有限公司 | Dioscorea composita cutting propagation method |
-
2015
- 2015-04-02 CN CN201510153737.1A patent/CN104718954A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102640630A (en) * | 2011-02-21 | 2012-08-22 | 北京灏銮科技发展有限公司 | Transplanting survival keeping method for honeysuckle |
| CN102640629A (en) * | 2011-02-21 | 2012-08-22 | 深圳市中科时代生物科技有限公司 | Dioscorea composita cutting propagation method |
Non-Patent Citations (1)
| Title |
|---|
| 陈润生: "中药之清补佳品太子参", 《首都医药》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103125244A (en) | Cutting propagation method of golden camellia plants | |
| CN114946512B (en) | Cultivation method for improving growth performance of garlic fruit plants | |
| CN102640629B (en) | Dioscorea composita cutting propagation method | |
| CN110526772B (en) | Antibacterial fertilizer for preventing and treating bacterial wilt of crops and preparation method thereof | |
| CN102668832B (en) | Pot culture method for persimmons | |
| CN103864519A (en) | Compound substrate for strawberry soilless potted plants | |
| CN112753393B (en) | Broussonetia papyrifera root propagation method | |
| CN108605617B (en) | Sowing and seedling raising method for European mountain ash facility | |
| CN104663191A (en) | Method for cultivating callicarpa nudiflora | |
| CN112042477A (en) | Jujun grass seedling cultivation method | |
| CN115152455B (en) | Method for producing cut lotus flowers | |
| JP3495976B2 (en) | Cultivation method of Japanese cabbage radish using deep sea water | |
| CN104160856B (en) | A kind of cultivation method improving kale cut-flower amount and production of hybrid seeds amount | |
| CN104429733A (en) | Method for preventing and controlling black shank of Honghua Dajinyuan | |
| CN110521413B (en) | Persimmon plant twig cutting seedling method | |
| CN107494089A (en) | A kind of the Northeast's rice specular removal planting technology | |
| CN104718954A (en) | Radix pseudostellariae cutting propagation method | |
| CN107333481A (en) | A kind of cultural method of Siberian iris | |
| CN110338064A (en) | A kind of culture of rootage and method for culturing seedlings of hemiplelea davidii tissue-cultured seedling | |
| CN104782618A (en) | Strawberry seedling tissue preservation method | |
| CN110291892A (en) | A kind of synthetic method for planting for preventing and treating Fructus Forsythiae pest and disease damage | |
| CN114052018B (en) | Agent for improving salt and alkali resistance of holboellia latifolia and treatment method | |
| CN113973821B (en) | Drought-resistant agent and method for improving drought resistance of plants | |
| Vamsi et al. | Studies on effect of different growing media combinations on growth, yield and quality of parthenocarpic cucumber (Cucumis sativus L.) in soilless cultivation under naturally ventilated polyhouse | |
| CN114041463B (en) | Agents and method for reduplicating and reducing damage and improving mutagenesis rate of gynura bicolor chromosome |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150624 |