CN104686657A - Formula donkey milk powder with anti-tumor efficacy - Google Patents
Formula donkey milk powder with anti-tumor efficacy Download PDFInfo
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Abstract
The invention relates to a formula donkey milk formula with an anti-tumor efficacy. The formula donkey milk powder is characterized in that donkey milk powder is taken as the main material, and components, including lactobacillus acidophilus, lactobacillus rhamnosus, lactobacillus plantarum, lentinan, pachymaran, grifolan, fructo-oligosaccharide, galactooligosaccharide, isomaltose hypgather, synanthrin, polysaccharides in reed rhizome, apricot kernel meal, walnut powder, Chinese yam powder, lotus seed powder, lily root flour, wolfberry powder and the like are added. The formula donkey milk powder has the effects of inhibiting the growth of tumor cells and prolonging the living time of tumor-bearing mice.
Description
Technical field
The invention belongs to healthcare field, specifically a kind of formula donkey milk powder with antitumor efficacy.
Background technology
Cancer is the disease that a kind of pathogenesis is very complicated, is one of principal disease of serious threat human life safety.Except the early-stage cancer of particular type, most cancer still cannot accomplish thorough healing.The mode of current Therapeutic cancer relates generally to chemicals, protein antagonist medicine, antibody drug, radiotherapy etc., though part treat mode Be very effective, often there is the problems such as side effect is large, price is high, is difficult to as long-term prevention and anticancer means.Therefore, prevention and auxiliary for treating cancer, improving patients ' life quality and extending life span becomes current antitumor research field major issue urgently to be resolved hurrily.The traditional Chinese medical science as the prevention of cancer and auxiliary treatment means known by people.By using " monarch, the minister, help, make " of the traditional Chinese medical science theoretical, in prior art, there is the pharmaceutical composition in a large number for cancer prevention and treatment.As patent CN 101530589 B discloses a kind of Chinese medicine preparation with pre-anti-cancer that is used for the treatment of, it is formulated that it comprises two Ten Herbs such as selected pearl, calculus bovis factitius, dragon's blood, pseudo-ginseng, muscone, borneol, amber, agalloch eaglewood, Tabasheer.Patent CN 101732535 B discloses a kind of Chinese medicine preparation being used for the treatment of and preventing cervical carcinoma, its with the red sage root, airpotato yam, nidus vespae, turtle shell, myrrh for monarch drug in a prescription, with cattail pollen, excrementum pteropi, monkshood, tarragon, snake fruit for ministerial drug, assistant is with setose thistle, subprostrate sophora, dried human placenta, selfheal, scorpio, make medicine with centipede, Toad Skin, the larva of a silkworm with batrytis, gecko, leech and Moschus, obtain described Chinese medicine preparation by scientific compatibility and preparation technology.Patent application CN1537565 A discloses the capsule of a kind for the treatment of or pre-anti-cancer, and it is made up of acer truncatum, radix cynanchi bungei, the bighead atractylodes rhizome, the root of herbaceous peony, Radix Glycyrrhizae traditional Chinese medicine of the five flavours material formula.Although the side effect of Chinese patent medicine preparation is less than radiotherapy or chemotherapy usually, price is also more cheap compared with radiotherapy, chemotherapeutics, but as cancer prevention and the therapeutic products of Long-Time Service, the preparation cost of Chinese medicine composition is still higher, and Long-Time Service still there will be some toxic and side effects, therefore Chinese patent medicine preparation is still not suitable as daily cancer prevention and therapeutic products Long-Time Service.
Dietotherapy is the marrow of Chinese medical, and it reaches the object for the treatment of, prevent disease (as cancer) by taking in beneficiating ingredient in food.Compared to Chinese medicine, dietotherapy not only side effect is little, and more easily approve by people acceptance, there are wide market prospects, thus become at present popular one for the treatment of, avoidance mode.
Donkey milk is the milk products closest to human milk, and its nutritive value is better than goat milk and people's milk, is considered to the substitute that can be used as breast milk.First, donkey milk is rich in essential fatty acid-linoleic acid plus linolenic acid, 3 times of in donkey milk, linolenic and linoleic accounts for the ratio of TFA to be 30.7% be about breast milk.In addition, donkey milk is also rich in selenium, and its Se content is 5.2 times of milk.Research shows, selenium is the indispensable material of human body, is called as " kindling material of life ".The generation of selenium energy exciting human immune protein antibiont, eliminates the material being unfavorable for human body, Cell protection film and chromosome, the effects such as the division of blocking-up cancer cell and growth.3rd, be rich in donkey milk and there is very high nutrition value and the whey egg being described as " king of protein " by nutrition educational circles of biological value.The content of the lactalbumin in donkey milk is far above people's milk, goat milk and milk.Donkey milk, except aforementioned important nutritive value, also has medical value widely.Just on the books in Compendium of Material Medica: " donkey breast, smell is sweet, cold profit, nontoxic, heat frequently drink can control the obstruction of the circulation of vital energy, separate children's's heat poison, do not give birth to acne rash ".Modern study shows, is rich in " epithelial cell growth factor " (EGF) in donkey milk, and the EGF factor has repairs the effect of nasal cavity tunica mucosa tracheae, and can strengthen resistance of human body, immunity.Medical expert's research is thought: suffer from bronchitis, pneumonia, pulmonary tuberculosis patient, treatments period drinks donkey milk adjuvant treatment effect.Can say that donkey milk is a kind of integration of drinking and medicinal herbs health care dairy products having commercial application prospect.In security, closely, available donkey milk replaces milk feeding infant for donkey milk and people's milk.Because in donkey milk, cholesterol level is only 20% of milk, therefore or diabetic, hyperpietic preferred drink.Visible, donkey milk a kind ofly has anti-cancer and cancer-preventing effect, nutritious, safety and the desirable anticancer health-care product that can drink for a long time.
At present about the research that donkey milk is anticancer is less, (the experimental study of fresh donkey milk in vivo antitumor effect such as Zhai Baoju, Zhai Baoju etc., Xinjiang Medicine University's journal, 34th volume the 3rd phase in 2011,274-278) have studied the effect of Tumor suppression growth in fresh donkey milk body, and show that fresh donkey milk has the effect suppressing S180 implanted solid tumor growth and extend the S180 ascites tumor mouse survival time by zoopery.Patent field there is not yet that donkey milk composition is used for the treatment of, the relevant report of pre-anti-cancer.In order to improve the antitumor efficacy of donkey milk further, the present inventor is by a large amount of basic theories and experimental study, creatively filter out the multiple component for the treatment of with donkey milk powder compatibility to strengthen donkey milk, preventing tumor function, and successfully have developed a kind of antitumor formula donkey milk powder newly, on the basis overcoming fresh donkey milk inconvenience storage and transport, significantly improve the antitumor efficacy of formula donkey milk powder.There is high market application foreground.
Summary of the invention
The object of the invention is to the antitumous effect improving existing donkey milk further, thus provide a kind of formula donkey milk powder with antitumor efficacy newly.
Concrete, a kind of formula donkey milk powder with antitumor efficacy newly of the present invention comprises following technical proposals:
A kind of formula donkey milk powder with antitumor efficacy, described formula donkey milk powder for add one or more (preferably two kinds in donkey milk powder, more preferably three kinds) probio, one or more (preferably three or more, preferably four kinds or more again, more preferably five kinds or more, more more preferably six kinds or more, optimum seven kinds or more and the most preferably or more than eight kinds) prebiotics, and be selected from almond powder, walnut powder, yam flour, lotus nut starch, lily root flour, one or more (preferably two kinds or more in wolfberry fruit powder, again preferably three or more, more preferably four kinds or more, more more preferably five kinds or more, most preferably six kinds) component.
Described probio is selected from: lactobacillus acidophilus (L.acidophilus), Lactobacillus rhamnosus (L.rhamnosus) or Lactobacillus plantarum (L.plantarum).
The viable count of described probiotics bacterial powder is about 4.5 × 10
10cfu/g ~ about 6.5 × 10
10cfu/g, and add in described donkey milk powder according to the dosage of about 0.25mg/g to about 0.65mg/g.
Described prebiotics is selected from: lentinan, pachymaran, grifolan, FOS, galactooligosaccharide, oligoisomaltose, synanthrin or reed rhizome polysaccharide.
The addition of described prebiotics all adds in described donkey milk powder by the dosage of about 0.6mg/g to about 1.5mg/g.
Described almond powder, walnut powder, yam flour, lotus nut starch, lily root flour, wolfberry fruit powder is respectively to the addition of described donkey milk powder: almond powder is about 0.2mg/g to about 0.4mg/g (preferred 0.3mg/g), walnut powder is about 0.3mg/g to about 0.5mg/g (preferred 0.4mg/g), yam flour is about 1.3mg/g to about 2mg/g (preferred 1.6mg/g), lotus nut starch is about 0.6mg/g to about 1.2mg/g (preferred 0.85mg/g), lily root flour is about 0.4mg/g to about 0.8mg/g (preferred 0.7mg/g), wolfberry fruit powder is about 0.6mg/g to about 1.0mg/g (preferred 0.8mg/g).
More specifically, the invention provides a kind of formula donkey milk powder with antitumor efficacy, described formula donkey milk powder for add following component in donkey milk powder: lactobacillus acidophilus 0.55mg/g, Lactobacillus rhamnosus 0.35mg/g, Lactobacillus plantarum 0.7mg/g, lentinan 0.9mg/g, pachymaran 0.6mg/g, grifolan 0.6mg/g, FOS 1.0mg/g, galactooligosaccharide 0.6mg/g, oligoisomaltose 1.1mg/g, synanthrin 0.65mg/g, reed rhizome polysaccharide 0.55mg/g, almond powder 0.2mg/g, walnut powder 0.3mg/g, yam flour 1.4mg/g, lotus nut starch 0.75mg/g, lily root flour 0.5mg/g and wolfberry fruit powder 0.6mg/g.
More specifically, the invention provides a kind of formula donkey milk powder with antitumor efficacy, described formula donkey milk powder for add following component in donkey milk powder: lactobacillus acidophilus 0.45mg/g, Lactobacillus rhamnosus 0.65mg/g, Lactobacillus plantarum 0.8mg/g, lentinan 0.75mg/g, pachymaran 0.7mg/g, grifolan 0.65mg/g, FOS 0.45mg/g, galactooligosaccharide 0.12mg/g, oligoisomaltose 0.7mg/g, synanthrin 0.95mg/g, reed rhizome polysaccharide 0.45mg/g, almond powder 0.35mg/g, walnut powder 0.5mg/g, yam flour 1.8mg/g, lotus nut starch 1.1mg/g, lily root flour 0.6mg/g and wolfberry fruit powder 0.7mg/g.
More specifically, the invention provides a kind of formula donkey milk powder with antitumor efficacy, described formula donkey milk powder for add following component in donkey milk powder: lactobacillus acidophilus 0.45mg/g, Lactobacillus rhamnosus 0.35mg/g, Lactobacillus plantarum 0.6mg/g, lentinan 0.85mg/g, pachymaran 0.8mg/g, grifolan 0.77mg/g, FOS 0.65mg/g, galactooligosaccharide 0.9mg/g, oligoisomaltose 0.9mg/g, synanthrin 0.85mg/g, reed rhizome polysaccharide 0.65mg/g, almond powder 0.3mg/g, walnut powder 0.4mg/g, yam flour 1.6mg/g, lotus nut starch 0.85mg/g, lily root flour 0.7mg/g and wolfberry fruit powder 0.8mg/g.
In another aspect of this invention, the application of a kind of aforementioned formula donkey milk powder in the product of preparation treatment, pre-anti-cancer/tumour is additionally provided.
Described cancer/tumour is selected from sarcoma, lung cancer, liver cancer, breast cancer, prostate cancer, colorectal cancer, the carcinoma of the rectum, neurogliocytoma etc.
In another aspect of this invention, additionally provide the preparation method of aforementioned formula donkey milk powder, described method comprises: add in donkey milk powder and be selected from probio described in one or more, one or more prebioticses described and one or more almond powder, walnut powder, yam flour, lotus nut starch, lily root flour, wolfberry fruit powder mixing.
Detailed description of the invention
Be described in detail for the preferred embodiments of the present invention below.Should be appreciated that preferred embodiment only for illustration of the present invention instead of restriction protection scope of the present invention.
embodiment 1 prepares the donkey milk powder of different formulations
Prepare the donkey milk powder of following different formulations, concrete formula sees the following form 1:
Embodiment 2 zoopery-formula donkey milk powder of the present invention comparatively donkey milk powder, has higher tumor inhibitory effect
2.1 material
Buy male mouse of kunming body weight 20 ± 2g and S180 tumor line.
2.2 method
Experiment grouping at random kunming mice is divided into following 11 groups: control group (physiological saline), donkey milk powder group, fill a prescription 1 group, fill a prescription 2 groups, fill a prescription 3 groups, fill a prescription 4 groups, fill a prescription 5 groups, fill a prescription 6 groups, fill a prescription 7 groups, fill a prescription 8 groups, fill a prescription 9 groups.Often organize 8 mouse.Wherein said donkey milk powder group, formula 1-9 group are referred to as treatment group.
Compound method by milk powder group and formula 1 to formula 9 groups formula milk composite be liquid milk.In all 11 groups, the gavage volume of every mouse is 0.4ml/ pcs/day.
Medication prepares S180 solid tumor mouse model.Within 2nd day, start to give the corresponding formulation of different group (the donkey milk that physiological saline (control group) is composite or composite formula 1-9 donkey milk) in inoculation, carry out gavage every day 11 up to 13 time, continue 3 weeks.
Within 1st day, put to death mouse after the gavage that detects tumor-inhibiting action terminates, peel off tumour and to weigh record, and with following formulae discovery tumour inhibiting rate (%):
2.3 experimental result
Table 2 illustrated gavage after 3 weeks, and the tumor weight of each group tumor-bearing mice and inhibiting rate (%), as can be seen from Table 2, the knurl inhibiting rate of donkey milk powder group and formula 1 group to 9 groups is all significantly higher than control group (P<0.05).In addition, 3 groups of knurl inhibiting rates to formula 9 groups of filling a prescription all are significantly higher than donkey milk powder group (P<0.05), the inhibition of 7-9 of wherein filling a prescription is the most obvious, formula donkey milk powder of the present invention comparatively donkey milk powder is described, having higher tumor inhibitory effect, is a kind of outstanding oncotherapy, prophylactic function formula donkey milk powder of having application prospect.
Table 2 gavage, after 3 weeks, respectively organizes tumor weight and the inhibiting rate (%) of tumor-bearing mice
*: compare P<0.05 with control group;
△: compare P<0.05 with donkey milk powder group.
Embodiment 3--formula of the present invention donkey milk powder comparatively donkey milk powder, extends the time-to-live of tumor-bearing mice further
3.1 material
Buy male mouse of kunming body weight 20 ± 2g and S180 tumor line.
3.2 method
Experiment grouping at random kunming mice is divided into following 11 groups: control group (physiological saline), donkey milk powder group, fill a prescription 1 group, fill a prescription 2 groups, fill a prescription 3 groups, fill a prescription 4 groups, fill a prescription 5 groups, fill a prescription 6 groups, fill a prescription 7 groups, fill a prescription 8 groups, fill a prescription 9 groups.Often organize 8 mouse.Wherein said donkey milk group, formula 1-9 group are referred to as treatment group.
Compound method by donkey milk powder group and formula 1 to formula 9 groups formula milk composite be liquid milk.In all 11 groups, the gavage volume of every mouse is 0.4ml/ pcs/day.
The S180 ascites tumor mouse model that medication preparation is transplanted.Each group of given low and method are with embodiment 2.The gavage duration, till dead mouse, records mouse diing time, according to formulae discovery increase in life span:
3.3 experimental result
Table 3 illustrates control group, donkey milk powder group and formula 1-9 group to the impact of S180 ascites tumor mouse survival time.As can be seen from Table 3, donkey milk powder group and formula 1-9 group are compared with control group equal significant prolongation the mean survival time of tumor-bearing mice (P<0.05), the mouse that 5-9 group of wherein filling a prescription is treated compared with donkey milk powder group, there is longer mean survival time (P<0.05) and higher rate elongation, the prolongation effect of 6-9 of wherein filling a prescription is the most obvious, formula donkey milk powder of the present invention comparatively donkey milk powder is described, extend the time-to-live of tumor-bearing mice further, it is a kind of outstanding oncotherapy having application prospect, prophylactic function formula donkey milk powder.
Table 3 control group, donkey milk powder group and formula 1-9 group are on the impact of S180 ascites tumor mouse survival time
*: compare P<0.05 with control group;
△: compare P<0.05 with donkey milk powder group.
Claims (10)
1. one kind has the formula donkey milk powder of antitumor efficacy, it is characterized in that described formula donkey milk powder for add one or more (preferably two kinds in donkey milk powder, more preferably three kinds) probio, one or more (preferably three or more, preferably four kinds or more again, more preferably five kinds or more, more more preferably six kinds or more, optimum seven kinds or more and the most preferably or more than eight kinds) prebiotics, and be selected from almond powder, walnut powder, yam flour, lotus nut starch, lily root flour, one or more (preferably two kinds or more in wolfberry fruit powder, again preferably three or more, more preferably four kinds or more, more more preferably five kinds or more, optimum excellent six kinds) component.
2. formula donkey milk powder according to claim 1, is characterized in that described probio is selected from: lactobacillus acidophilus (
l.acidophilus), Lactobacillus rhamnosus (
l.rhamnosus) or Lactobacillus plantarum (
l.plantarum).
3. formula donkey milk powder according to claim 2, is characterized in that the viable count of described probiotics bacterial powder is about 4.5 × 10
10cfu/g ~ about 6.5 × 10
10cfu/g, and add in described donkey milk powder according to the dosage of about 0.25mg/g to about 0.65mg/g.
4. the formula donkey milk powder according to claim 1-3, is characterized in that described prebiotics is selected from: lentinan, pachymaran, grifolan, FOS, galactooligosaccharide, oligoisomaltose, synanthrin or reed rhizome polysaccharide; Preferably, the addition of described prebiotics all adds in described donkey milk powder by the dosage of about 0.6 mg/g to about 1.5mg/g.
5. according to the arbitrary described formula donkey milk powder of claim 1-4, it is characterized in that described almond powder, walnut powder, yam flour, lotus nut starch, lily root flour, wolfberry fruit powder is respectively to the addition of described donkey milk powder: almond powder is about 0.2mg/g to the preferred 0.3mg/g of about 0.4mg/g(), walnut powder is about 0.3mg/g to the preferred 0.4mg/g of about 0.5mg/g(), yam flour is about 1.3mg/g to the preferred 1.6mg/g of about 2mg/g(), lotus nut starch is about 0.6mg/g to the preferred 0.85mg/g of about 1.2mg/g(), lily root flour is about 0.4mg/g to the preferred 0.7mg/g of about 0.8mg/g(), wolfberry fruit powder is about 0.6mg/g to the preferred 0.8mg/g of about 1.0mg/g().
6. have a formula donkey milk powder for antitumor efficacy, described formula donkey milk powder for add following component in donkey milk powder: lactobacillus acidophilus (
l.acidophilus) 0.55mg/g, Lactobacillus rhamnosus (
l.rhamnosus) 0.35 mg/g, Lactobacillus plantarum (
l.plantarum) 0.7 mg/g, lentinan 0.9 mg/g, pachymaran 0.6 mg/g, grifolan 0.6 mg/g, FOS 1.0 mg/g, galactooligosaccharide 0.6 mg/g, oligoisomaltose 1.1 mg/g, synanthrin 0.65 mg/g, reed rhizome polysaccharide 0.55 mg/g, almond powder 0.2 mg/g, walnut powder 0.3 mg/g, yam flour 1.4 mg/g, lotus nut starch 0.75 mg/g, lily root flour 0.5 mg/g and wolfberry fruit powder 0.6 mg/g.
7. one kind has the formula donkey milk powder of antitumor efficacy, described formula donkey milk powder for add following component in donkey milk powder: lactobacillus acidophilus 0.45 mg/g, Lactobacillus rhamnosus 0.65 mg/g, Lactobacillus plantarum 0.8 mg/g, lentinan 0.75 mg/g, pachymaran 0.7 mg/g, grifolan 0.65 mg/g, FOS 0.45 mg/g, galactooligosaccharide 0.12 mg/g, oligoisomaltose 0.7 mg/g, synanthrin 0.95 mg/g, reed rhizome polysaccharide 0.45 mg/g, almond powder 0.35 mg/g, walnut powder 0.5 mg/g, yam flour 1.8 mg/g, lotus nut starch 1.1 mg/g, lily root flour 0.6 mg/g and wolfberry fruit powder 0.7 mg/g.
8. one kind has the formula donkey milk powder of antitumor efficacy, described formula donkey milk powder for add following component in donkey milk powder: lactobacillus acidophilus 0.45 mg/g, Lactobacillus rhamnosus 0.35 mg/g, Lactobacillus plantarum 0.6 mg/g, lentinan 0.85 mg/g, pachymaran 0.8 mg/g, grifolan 0.77 mg/g, FOS 0.65 mg/g, galactooligosaccharide 0.9 mg/g, oligoisomaltose 0.9 mg/g, synanthrin 0.85 mg/g, reed rhizome polysaccharide 0.65 mg/g, almond powder 0.3 mg/g, walnut powder 0.4 mg/g, yam flour 1.6 mg/g, lotus nut starch 0.85 mg/g, lily root flour 0.7 mg/g and wolfberry fruit powder 0.8 mg/g.
9. the formula donkey milk powder described in an any one of claim 1-8 prevents and/or treats the application in the product of cancer/tumour (as sarcoma, lung cancer, liver cancer, breast cancer, prostate cancer, colorectal cancer, the carcinoma of the rectum, neurogliocytoma etc.) in preparation.
10. the preparation method of the formula donkey milk powder described in claim 1-8, comprising: in donkey milk powder, add corresponding described probio, described prebiotics and described almond powder, walnut powder, yam flour, lotus nut starch, lily root flour, wolfberry fruit powder mixing.
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CN105076438A (en) * | 2015-07-20 | 2015-11-25 | 济南伟传信息技术有限公司 | Nutritional health-care formula milk powder |
CN109645135A (en) * | 2019-01-21 | 2019-04-19 | 西北师范大学 | A kind of preparation method of fructus lycii donkey milk piece |
US11376297B2 (en) | 2017-11-09 | 2022-07-05 | Société des Produits Nestlé S.A. | Nutrition blend for health benefits in animals |
CN115299608A (en) * | 2022-08-01 | 2022-11-08 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Application of grifola frondosa polysaccharide F2 in preparation of intestinal prebiotics |
RU2784353C2 (en) * | 2017-11-09 | 2022-11-23 | Сосьете Де Продюи Нестле С.А. | Nutritional mixture for achievement of healthy effects in aminals |
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CN103704348A (en) * | 2013-12-18 | 2014-04-09 | 南昌大学 | Donkey milk powder formula for boosting brain, nourishing blood and improving resistance based on homology of medicine and food |
CN103704349A (en) * | 2013-12-18 | 2014-04-09 | 南昌大学 | Donkey milk powder formula based on medicine food homology |
CN103734338A (en) * | 2013-12-18 | 2014-04-23 | 南昌大学 | Compounded donkey milk powder for improving immunity |
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CN103651847A (en) * | 2013-12-18 | 2014-03-26 | 南昌大学 | Formula donkey milk powder with function of coordinating intestinal flora |
CN103704348A (en) * | 2013-12-18 | 2014-04-09 | 南昌大学 | Donkey milk powder formula for boosting brain, nourishing blood and improving resistance based on homology of medicine and food |
CN103704349A (en) * | 2013-12-18 | 2014-04-09 | 南昌大学 | Donkey milk powder formula based on medicine food homology |
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
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CN105076438A (en) * | 2015-07-20 | 2015-11-25 | 济南伟传信息技术有限公司 | Nutritional health-care formula milk powder |
US11376297B2 (en) | 2017-11-09 | 2022-07-05 | Société des Produits Nestlé S.A. | Nutrition blend for health benefits in animals |
RU2784353C2 (en) * | 2017-11-09 | 2022-11-23 | Сосьете Де Продюи Нестле С.А. | Nutritional mixture for achievement of healthy effects in aminals |
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CN109645135A (en) * | 2019-01-21 | 2019-04-19 | 西北师范大学 | A kind of preparation method of fructus lycii donkey milk piece |
CN115299608A (en) * | 2022-08-01 | 2022-11-08 | 广东省科学院微生物研究所(广东省微生物分析检测中心) | Application of grifola frondosa polysaccharide F2 in preparation of intestinal prebiotics |
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Application publication date: 20150610 |
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