CN103734338A - Compounded donkey milk powder for improving immunity - Google Patents
Compounded donkey milk powder for improving immunity Download PDFInfo
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Abstract
The invention discloses compounded donkey milk powder for improving immunity. According to the formula, donkey milk powder is used as a main raw material, Chinese yam, lily, walnuts and lobed kudzuvine root, which can be eaten as both medicines and food, are added, and prebiotics with fructo-oligose and galacto-oligosaccharide, and two probiotics, namely, lactobacillus rhamnosus and lactobacillus plantarum, are additionally supplemented. By virtue of the compounded donkey milk powder, the immunity can be remarkably improved, specifically, the conversion capability of lymphocyte (cellular immune function) is remarkably improved, the production of serum hemolysin (humoral immunity function) is increased, and the carbon clearance capability (mononuclear macrophage function) and the killing activity of NK (Natural Killer) cells (NK cell activity) are enhanced.
Description
Technical field
The invention belongs to healthcare field, is a kind of formula donkey milk powder that strengthens immunity of organisms specifically.
Background technology
The nutritional labeling of donkey milk is comprehensive, the most approaching with human milk.Amino acid whose A wide selection of colours and designs in donkey milk proem matter, quantity abundance, the ratio that 9 kinds of essential amino acids (histidine is baby's essential amino acid) such as its lysine, tryptophan, phenylalanine, methionine (methionine), threonine, isoleucine, leucine, valine, histidine account for total amino acid is 40.8%, close with human milk (43%).Aliphatic acid is the chief component composition of butterfat, and wherein linoleic acid and alpha-linolenic acid are essential fatty acid (EFA).Human body lacks essential fatty acid (EFA) can slow down organism metabolism, causes poor growth, the various diseases such as dysgenesia.Donkey breast is rich in EFA, and content is about 40 mg/100 g, especially linoleic acid, its content be in cow's milk 2.5 times of linoleic acid content (15 mg/100 g).In donkey milk, not only contain multiple macroelement, and be rich in trace element.In macroelement, Ca rich content, Na, Mg take second place; In trace element, Se content is higher, is 5.2 times of milk.Fine difference between donkey milk and human milk constituent, adjusts by batching than being easier to.But, at present the research of donkey milk is mainly concentrated on the analysis of its nutritional labeling, and lack on aspect the comprehensive development and utilization of donkey milk, go deep into systematic research.Therefore, carry out the applied basic research of donkey milk powder compound prescription, widen its application approach, there are important economic results in society.
Summary of the invention
The invention provides following technology path:
The present invention is intended to provide new way for the market development of donkey milk, take donkey milk powder as matrix, add Chinese yam, lily, walnut and the root of kudzu vine that integration of drinking and medicinal herbs can be used, and be aided with prebiotics and Lactobacillus rhamnosus and two kinds of probios of Lactobacillus plantarum of adding containing FOS and galactooligosaccharide.The present invention includes following technical scheme:
Improve a formula donkey milk powder for immunity, according to following steps, prepare:
(1) the fresh Chinese yam of selecting profile rounding, smooth surface, go rotten without insect pest is as raw materials; First wash away the earth on Chinese yam surface, with beam knife, Chinese yam epidermis is removed to clean also water and rinsed; With stainless steel knife, the Chinese yam after peeling is cut to the thin slice of 1 about cm, and puts into rapidly clear water and soak, it is quick that section is wanted, so as not in air open-assembly time longly cause brown stain; Yam slices protects look 10 min left and right in 0.2 g/100 mL citric acid colour protecting liquid at (98 ± 2) ℃, can obtain well protecting chromatic effect, also can make raw material be mature on the whole and obtain strong Chinese yam local flavor; After protecting look, with clear water, rinse immediately, to remove the citric acid sticking; In yam slice, add the water of 3 times of quality, be ground into pulpous state with pulverizer; Chinese yam slurry is added in donkey milk by 3.6 mg/g dosage; Be that every g donkey milk adds 3.6 mg Chinese yam slurries;
(2) get new fresh lily bulb, select lily large and neat, pure white plumpness, reject lily mildew and rot, sick worm; Remove peripheral withered old scale and stem chassis, clean up with circulating water; Get the lily of cleaning, add 5 times of water, by 100 g lilies, add the citric acid of the different VC-Na of 0.4 g and 0.1%, be heated to 100 ℃ process 5～10 min, to lily bright transparent till, cooling immediately, carry out crushing and beating; With milling treatment of colloid lily, starch 2 times, extremely without lily coarse granule; The pH value to 6.0 that regulates lily slurry, adds high temperature resistant AMS, and consumption is 200 U/g, and 25 min liquefy at 95 ℃; The pH to 5.5 regulating, adds carbohydrase, and consumption is 100～300 U/g, processes 1～3 h at 60～70 ℃; Through the lily hydrolyzate of enzymolysis processing, at 100 ℃, keep the 5 min enzyme processing of going out; The lily slurry of the enzyme processing of going out is added in donkey milk by 7.2 mg/g;
(3) select that meat is full, not damaged free from insect pests, without the fresh walnut kernel going mouldy, remove the impurity such as broken shell and barrier film, then use clear water rinsing walnut kernel; Walnut kernel benevolence is boiled after 3 min with weak acid neutralization with 0.2% NaOH, and then water rinses; Slough the walnut kernel of seed coat and smash to pieces in high-speed tissue mashing machine, solid-liquid ratio is 1:4, then uses colloid mill fine grinding; Colloid mill spacing size suitably, is generally selected 5～8 μ m; Walnut powder after grinding is added in donkey milk by 3.0 mg/g dosage;
(4) take kudzu-vine root powder approximately 2 g that handle well, pour in lixiviate reflux, add the ethanol that 60 mL concentration are 50%, wherein solid-to-liquid ratio is 1:30, lixiviate 3 h that reflux in the water-bath of 90 ℃, suction filtration is also collected filtrate, and the clarified solution that suction filtration is obtained proceeds in brown bottle to be preserved; Kudzu root flavone adds in donkey milk by 0.24 mg/g dosage;
(5) witloof FOS adds donkey milk powder to by 0.985 mg/g dosage;
(6) preparation of probio freeze drying viable microorganism powder: first prepare mass percent concentration and be 10% defatted milk emulsion, be sub-packed in 4 500 mL triangular flasks, every bottle of 200 mL, 121 ℃ of sterilizing 20 min are cooled to 37 ℃, respectively by 2% volume ratio inoculation Lactobacillus rhamnosus and Lactobacillus plantarum, 37 ℃ of anaerobism are cultivated 24-28 h, detect the viable count of 2 probiotics nutrient solutions, each nutrient solution viable count >10
9cFU/mL, can stop cultivating, if viable count <10
9cFU/mL, continues to cultivate until reach 10
9cFU/mL; Nutrient solution is imported in glass ampoule under aseptic condition, and liquid level 0.8 cm to 1 cm, puts into-30 ℃ of refrigerator-freezer quick-frozens after adding a cover bottle stopper, after freezing, glass ampoule is used to pallet splendid attire, puts into freeze dryer and carries out freeze drying, prepares powder freeze-drying lactobacillus; Lactobacillus rhamnosus freeze-dried vaccine powder adds in donkey milk by 0.4 mg/g dosage, and Lactobacillus plantarum adds in donkey milk by 0.2 mg/g dosage;
(7) the donkey milk mixture that has added above-mentioned material is carried out to homogeneous, homogenization pressure and temperature are respectively 20-25 MPa and 45-50 ℃; By the donkey milk mixture after homogeneous, spray dry; Spraying drying condition control is: EAT is 130-160 ℃ and is preferably 150 ℃, and tower temperature is 75 ℃, and temperature of outgoing air is 70-75 ℃.
For evaluating the present invention, on the basis of donkey milk powder, further strengthen immunity of organisms, by gavage that immunocompromised mouse is divided into groups, containing physiological saline group, donkey milk powder group and formula donkey milk powder group.
Adopting intraperitoneal injection of cyclophosphamide to prepare after immunocompromised mouse, by gavage group, distinguish continuous gavage 28 days.After gavage finishes, get mouse spleen, grind and obtain SPL, add ConA and stimulate lymphocyte, observe the lymphocytic conversion capability of different gavage group mouse.After gavage finishes, by tail vein injection prepared Chinese ink, evaluate the carbon of different gavage group mouse and clean up ability; Before gavage finishes the 4th day, lumbar injection Mianyang erythrocyte is got mouse spleen and is ground and obtain SPL after gavage finishes, and evaluates the kill capability of NK cell to target cell YAC-1; Before gavage finishes the 4th day, lumbar injection Mianyang erythrocyte is got blood after gavage finishes, and measures the generation of serum hemolysin.
Beneficial effect of the present invention:
1, the present invention's donkey milk powder of filling a prescription has the effect of immunity of raising;
2, the present invention's donkey milk powder taste of filling a prescription is pure and fresh, and mouthfeel is good, is more easily that ruck is accepted, and is easy to promote.
The specific embodiment
Embodiment 1
Improve a formula donkey milk powder for immunity, according to following steps, prepare:
(1) the fresh Chinese yam of selecting profile rounding, smooth surface, go rotten without insect pest is as raw materials; First wash away the earth on Chinese yam surface, with beam knife, Chinese yam epidermis is removed to clean also water and rinsed; With stainless steel knife, the Chinese yam after peeling is cut to the thin slice of 1 about cm, and puts into rapidly clear water and soak, it is quick that section is wanted, so as not in air open-assembly time longly cause brown stain; Yam slices protects look 10 min left and right in 0.2 g/100 mL citric acid colour protecting liquid at (98 ± 2) ℃, can obtain well protecting chromatic effect, also can make raw material be mature on the whole and obtain strong Chinese yam local flavor; After protecting look, with clear water, rinse immediately, to remove the citric acid sticking; In yam slice, add the water of 3 times of quality, be ground into pulpous state with pulverizer; Chinese yam slurry is added in donkey milk by 3.6 mg/g dosage; Be that every g donkey milk adds 3.6 mg Chinese yam slurries;
(2) get new fresh lily bulb, select lily large and neat, pure white plumpness, reject lily mildew and rot, sick worm; Remove peripheral withered old scale and stem chassis, clean up with circulating water; Get the lily of cleaning, add 5 times of water, by 100 g lilies, add the citric acid of the different VC-Na of 0.4 g and 0.1%, be heated to 100 ℃ process 5～10 min, to lily bright transparent till, cooling immediately, carry out crushing and beating; With milling treatment of colloid lily, starch 2 times, extremely without lily coarse granule; The pH value to 6.0 that regulates lily slurry, adds high temperature resistant AMS, and consumption is 200 U/g, and 25 min liquefy at 95 ℃; The pH to 5.5 regulating, adds carbohydrase, and consumption is 100～300 U/g, processes 1～3 h at 60～70 ℃; Through the lily hydrolyzate of enzymolysis processing, at 100 ℃, keep the 5 min enzyme processing of going out; The lily slurry of the enzyme processing of going out is added in donkey milk by 7.2 mg/g;
(3) select that meat is full, not damaged free from insect pests, without the fresh walnut kernel going mouldy, remove the impurity such as broken shell and barrier film, then use clear water rinsing walnut kernel; Walnut kernel benevolence is boiled after 3 min with weak acid neutralization with 0.2% NaOH, and then water rinses; Slough the walnut kernel of seed coat and smash to pieces in high-speed tissue mashing machine, solid-liquid ratio is 1:4, then uses colloid mill fine grinding; Colloid mill spacing size suitably, is generally selected 5～8 μ m; Walnut powder after grinding is added in donkey milk by 3.0 mg/g dosage;
(4) take kudzu-vine root powder approximately 2 g that handle well, pour in lixiviate reflux, add the ethanol that 60 mL concentration are 50%, wherein solid-to-liquid ratio is 1:30, lixiviate 3 h that reflux in the water-bath of 90 ℃, suction filtration is also collected filtrate, and the clarified solution that suction filtration is obtained proceeds in brown bottle to be preserved; Kudzu root flavone adds in donkey milk by 0.24 mg/g dosage;
(5) witloof FOS adds donkey milk powder to by 0.985 mg/g dosage;
(6) preparation of probio freeze drying viable microorganism powder: first prepare mass percent concentration and be 10% defatted milk emulsion, be sub-packed in 4 500 mL triangular flasks, every bottle of 200 mL, 121 ℃ of sterilizing 20 min are cooled to 37 ℃, respectively by 2% volume ratio inoculation Lactobacillus rhamnosus and Lactobacillus plantarum, 37 ℃ of anaerobism are cultivated 24-28 h, detect the viable count of 2 probiotics nutrient solutions, each nutrient solution viable count >10
9cFU/mL, can stop cultivating, if viable count <10
9cFU/mL, continues to cultivate until reach 10
9cFU/mL; Nutrient solution is imported in glass ampoule under aseptic condition, and liquid level 0.8 cm to 1 cm, puts into-30 ℃ of refrigerator-freezer quick-frozens after adding a cover bottle stopper, after freezing, glass ampoule is used to pallet splendid attire, puts into freeze dryer and carries out freeze drying, prepares powder freeze-drying lactobacillus; Lactobacillus rhamnosus freeze-dried vaccine powder adds in donkey milk by 0.4 mg/g dosage, and Lactobacillus plantarum adds in donkey milk by 0.2 mg/g dosage;
(7) the donkey milk mixture that has added above-mentioned material is carried out to homogeneous, homogenization pressure and temperature are respectively 20-25 MPa and 45-50 ℃; By the donkey milk mixture after homogeneous, spray dry; The control of spraying drying condition is: EAT is 130-160 ℃, and tower temperature is 75 ℃, and temperature of outgoing air is 70-75 ℃.
Embodiment 2 immunity tests
The preparation of immunocompromised mouse: buy 72 of BALB/c mouses, place Animal House and observe after one week, the next day intraperitoneal injection of cyclophosphamide solution, first day is by 100 mg/kg dosage injections, the 3rd day by 80 mg/kg dosage injections, preparation immunocompromised mouse.
The setting of mouse stomach group: mouse is divided into 3 groups at random, 10 every group, is followed successively by blank group, donkey milk group, formula donkey milk powder group.Every group of mouse stomach phase is 28 days, and the gavage amount of every mouse was 300 μ L/ days.The SPSS of the mouse stomach 0.85% of blank group, the mouse of donkey milk powder group and formula donkey milk powder group (getting formula donkey milk powder prepared by embodiment 1) is by the dosage gavage of 5 g/kg.
The preparation of splenocyte suspension: adopt strength vertebra dislocation method to put to death mouse, in alcohol immersion 2 min with 75%, disinfect.Mouse is placed in and dissects pallet, along peritonaeum, dissect mouse, the aseptic spleen of getting, is placed in and fills appropriate aseptic Hank's liquid plate, removes the connective tissue of adhesion on spleen, and after washing, spleen is transferred in aseptic Hank's liquid plate gently.Spleen is ground gently on 40 order gauzes with tweezers, after 200 eye mesh screens filter, obtain single cell suspension.Cell suspension, after centrifugal 10 min of 1000 r/min, adds erythrocyte cracked liquid in 10:1 ratio, and piping and druming mixes gently, pyrolysis time approximately 5 min left and right.Centrifugal 10 min of 1000 r/min, remove supernatant, aseptic Hank's liquid washing 2 times for cell precipitation.Cell is suspended in 1 mL RPMI1640 complete culture solution, with platform, expects blue dyeing counting viable count (should more than 95%), adjusting cell concentration is 3 × 10
6individual/mL.
The mensuration of cellular immune function---mouse lymphocyte transformation experiment: divide 4 holes to add in 24 porocyte culture plates every a splenocyte suspension, every hole 1 mL, wherein 3 holes are as Duplicate Samples hole, all add 50 μ L ConA liquid (being equivalent to 5 μ g/mL), in residue 1 hole, add 50 μ L cell culture fluids to replace ConA liquid as blank well.Put 5% CO
2, in 37 ℃ of incubators, cultivate 72 h; Cultivation finishes front 4 h, and every hole sucks 0.7 mL supernatant gently, then adds 0.7 mL not containing the RPMI1640 nutrient solution of serum, and every hole adds 50 μ L MTT solution (5 mg/mL) simultaneously, continues to cultivate 4 h.After cultivation finishes, every hole adds l mL acid isopropyl alcohol, and fully piping and druming mixes, and purple crystal is dissolved completely.Then divide and install in 96 well culture plates, 3 parallel holes are made in each hole, measure extinction OD value the record in each hole under ELIASA 570 nm wavelength.Experimental result is as shown in table 1, result shows: compared with physiological saline group, the donkey milk powder of gavage one-component can not improve the conversion ratio (p>0.05) of SPL, and the SPL conversion ratio of gavage formula donkey milk powder group mouse is significantly higher than the mouse (p<0.05) of donkey milk powder group.
The impact (X ± SD) that the different gavage group of table 1 transforms the SPL of Con-A induction
Note: a represents compared with blank group, p<0.05, and b represents compared with donkey milk powder group, p<0.05.
The mensuration of humoral immune function---serum hemolysin experiment: mouse is carried out the SRBC of lumbar injection 0.2 mL 2% when gavage the 28th day, immunity taked eye socket to get blood after the 5th day.By the blood sample of collecting, in 37 ℃ of standing 1 h, 2,000 4 ℃ of r/min refrigerated centrifuge, 10 min, carefully draw serum, avoid sucking erythrocyte.Serum supplies to measure with 200 times of SA buffer solution dilutions.In example reaction pipe, add successively the mice serum of l mL dilution, 0.5 mL SRBC(10%), the complement of l mL dilution; Blank Guan Zhongyong 1 mL SA buffer solution replaces blood serum sample, after fully mixing, in 37 ℃ of waters bath with thermostatic control, is incubated 30 min, moves to cessation reaction in ice bath.Centrifugal 10 min of 2000 r/min, get l mL supernatant and add 3 mL Dou Shi reagent, shake up and place 10 min.With blank pipe, make blank, 540 nm wavelength colorimetrics read absorbance.The mensuration of SRBC HD50 value: get 0.25 mL SRBC(5%) add Dou Shi liquid to 4 mL, 540 nm wavelength colorimetrics read absorbance, the absorbance while being SRBC HD50 used in experiment.Example reaction pipe HD50 value CH
50be calculated as follows: the change of serum C H of every mouse
50absorbance × extension rate during absorbance/SRBC HD50 of=sample.Experimental result is as shown in table 2, result shows: aspect the generation of mice serum hemolysin, compared with the blank group of physiological saline, single donkey milk powder group all has significant difference (p<0.05) with formula donkey milk powder group, but formula donkey milk powder group increases the generation of serum hemolysin significantly than single donkey milk powder group.
The impact (X ± SD) of the different gavage group of table 2 on mice serum hemolysin
Note: a represents compared with blank group, p<0.05, and b represents compared with donkey milk powder group, p<0.05.
The mensuration of monocytes/macrophages phagocytic activity---mouse carbon is cleaned up experiment: injection prepared Chinese ink: by 0.1 mL/kg dosage from mouse tail vein injection prepared Chinese ink, after Ink injection, timing immediately.2 min and 8 min after injection prepared Chinese ink, get blood 20 μ L from angular vein clump respectively, and existing side by side, soon it is added to 2 mL 0.1% Na
2cO
3in solution, fully mix.In 600 nm wavelength place's photometry density value OD values, with Na
2cO
3solution is made blank.Mouse is put to death, get liver and spleen, blot organ surface blood stains, the record of weighing respectively with filter paper.With phagocytic index a, represent the ability that mouse carbon is cleaned up, be calculated as follows phagocytic index a:
phagocytic index a=
Experimental result is as shown in table 3, and result shows: formula donkey milk powder group can significantly strengthen mouse carbon and clean up ability (p<0.05).
The different gavage group of table 3 is cleaned up ability impact (X ± SD) on mouse carbon
| Group | Dosage | Size of animal/only | Phagocytic index/k | Proofread and correct phagocytic index/a |
| Blank group | 0.85% physiological saline | 10 | 0.019±0.003 | 5.008±0.538 |
| Single donkey milk powder group | 5 g/kg | 10 | 0.026±0.010 | 5.716±0.643 |
| Formula donkey milk powder group | 5 g/kg | 10 | 0.037±0.012 | 6.817±0.568 a b |
Note: a represents compared with blank group, p<0.05, and b represents compared with donkey milk powder group, p<0.05.
The mensuration of NK cytoactive---lactic dehydrogenase (LDH) determination method: on 96 porocyte culture plates, add effector cell (2 × 10 in experimental port
7individual/mL) and target cell (4 × 10
5individual/mL) each 100 μ L(effect target ratio=50:1), in target cell Spontaneous release hole, adding the each 100 μ L of target cell and nutrient solution, the maximum release aperture of target cell adds target cell and the each 100 μ L of 2.5% Triton, above-mentioned every three parallel holes of all establishing.In 37 ℃, 5% CO
2in incubator, cultivate 4 h; By 96 well culture plates, with centrifugal 10 min of 1500 r/min, supernatant 100 μ L are drawn in every hole, add LDH matrix liquid 100 μ L simultaneously, and after reaction 10 min, every hole adds the HCl(1 M of 30 μ L), at ELIASA, 490 nm places measure OD value record.Experimental result is as shown in table 4, result shows: formula donkey milk powder group has humidification (p<0.05) significantly to NK cells in mice killing activity, though and the donkey milk powder of one-component can improve its killing activity, its effect is remarkable (p>0.05) not.
The impact (X ± SD) of the different gavage groups of table 4 on NK cells in mice killing activity
| Group | Dosage | Size of animal/only | Activity/the % of NK cell |
| Blank group | 0.85% physiological saline | 10 | 18.813±2.288 |
| Donkey milk powder group | 5 g/kg | 10 | 23.673±4.789 |
| Formula donkey milk powder group | 5 g/kg | 10 | 31.544±4.098 a b |
Note: a represents compared with blank group, p<0.05, and b represents compared with donkey milk powder group, p<0.05.
Claims (2)
1. a formula donkey milk powder that improves immunity, is characterized in that preparing according to following steps:
(1) the fresh Chinese yam of selecting profile rounding, smooth surface, go rotten without insect pest is as raw materials; First wash away the earth on Chinese yam surface, with beam knife, Chinese yam epidermis is removed to clean also water and rinsed; With stainless steel knife, the Chinese yam after peeling is cut to the thin slice of 1 cm left and right, and puts into rapidly clear water and soak; Yam slices protects look 10 min left and right in 0.2 g/100 mL citric acid colour protecting liquid at (98 ± 2) ℃; After protecting look, with clear water, rinse immediately, to remove the citric acid sticking; In yam slice, add the water of 3 times of quality, be ground into pulpous state with pulverizer; Chinese yam slurry is added in donkey milk by 3.6 mg/g dosage; Be that every g donkey milk adds 3.6 mg Chinese yam slurries;
(2) get new fresh lily bulb, select lily large and neat, pure white plumpness, reject lily mildew and rot, sick worm; Remove peripheral withered old scale and stem chassis, clean up with circulating water; Get the lily of cleaning, add 5 times of water, by 100 g lilies, add the citric acid of the different VC-Na of 0.4 g and 0.1%, be heated to 100 ℃ process 5～10 min, to lily bright transparent till, cooling immediately, carry out crushing and beating; With milling treatment of colloid lily, starch 2 times, extremely without lily coarse granule; The pH value to 6.0 that regulates lily slurry, adds high temperature resistant AMS, and consumption is 200 U/g, and 25 min liquefy at 95 ℃; The pH to 5.5 regulating, adds carbohydrase, and consumption is 100～300 U/g, processes 1～3 h at 60～70 ℃; Through the lily hydrolyzate of enzymolysis processing, at 100 ℃, keep the 5 min enzyme processing of going out; The lily slurry of the enzyme processing of going out is added in donkey milk by 7.2 mg/g;
(3) select that meat is full, not damaged, free from insect pests, without the fresh walnut kernel going mouldy, remove the impurity such as broken shell and barrier film, then use clear water rinsing walnut kernel; Walnut kernel benevolence is boiled after 3 min with weak acid neutralization with 0.2% NaOH, and then water rinses; Slough the walnut kernel of seed coat and smash to pieces in high-speed tissue mashing machine, solid-liquid ratio is 1:4, then uses colloid mill fine grinding; Colloid mill spacing is selected 5～8 μ m; Walnut powder after grinding is added in donkey milk by 3.0 mg/g dosage;
(4) take kudzu-vine root powder approximately 2 g that handle well, pour in lixiviate reflux, add the ethanol that 60 mL concentration are 50%, wherein solid-to-liquid ratio is 1:30, lixiviate 3 h that reflux in the water-bath of 90 ℃, suction filtration is also collected filtrate, and the clarified solution that suction filtration is obtained proceeds in brown bottle to be preserved; Kudzu root flavone adds in donkey milk by 0.24 mg/g dosage;
(5) witloof FOS adds donkey milk powder to by 0.985 mg/g dosage;
(6) preparation of probio freeze drying viable microorganism powder: first prepare mass percent concentration and be 10% defatted milk emulsion, be sub-packed in 4 500 mL triangular flasks, every bottle of 200 mL, 121 ℃ of sterilizing 20 min are cooled to 37 ℃, respectively by 2% volume ratio inoculation Lactobacillus rhamnosus and Lactobacillus plantarum, 37 ℃ of anaerobism are cultivated 24-28 h, detect the viable count of 2 probiotics nutrient solutions, each nutrient solution viable count >10
9cFU/mL, can stop cultivating, if viable count <10
9cFU/mL, continues to cultivate until reach 10
9cFU/mL; Nutrient solution is imported in glass ampoule under aseptic condition, and liquid level 0.8 cm to 1 cm, puts into-30 ℃ of refrigerator-freezer quick-frozens after adding a cover bottle stopper, after freezing, glass ampoule is used to pallet splendid attire, puts into freeze dryer and carries out freeze drying, prepares powder freeze-drying lactobacillus; Lactobacillus rhamnosus freeze-dried vaccine powder adds in donkey milk by 0.4 mg/g dosage, and Lactobacillus plantarum adds in donkey milk by 0.2 mg/g dosage;
(7) the donkey milk mixture that has added above-mentioned material is carried out to homogeneous, homogenization pressure and temperature are respectively 20-25 MPa and 45-50 ℃; By the donkey milk mixture after homogeneous, spray dry.
2. formula donkey milk powder as claimed in claim 1, is characterized in that the control of step (7) spraying drying condition is: EAT is 130-160 ℃, is preferably 150 ℃, and tower temperature is 75 ℃, and temperature of outgoing air is 70-75 ℃.
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| CN104206642A (en) * | 2014-09-10 | 2014-12-17 | 青河县东惠绿叶养殖专业合作社 | Preparation process of donkey milk walnut protein powder and donkey milk walnut protein powder product |
| CN104686657A (en) * | 2015-02-11 | 2015-06-10 | 新疆世全黄金矿业股份有限公司 | Formula donkey milk powder with anti-tumor efficacy |
| CN104770469A (en) * | 2015-04-17 | 2015-07-15 | 武汉百信正源生物技术工程有限公司 | Method for preparing donkey yoghourt for increasing immunity function |
| CN105053190A (en) * | 2015-07-13 | 2015-11-18 | 王学香 | Health-care donkey milk drink for women and preparation method thereof |
| CN106259957A (en) * | 2016-08-28 | 2017-01-04 | 新疆玉昆仑天然食品工程有限公司 | A kind of lyophilizing donkey milk powder and preparation method thereof |
| CN106306028A (en) * | 2016-08-28 | 2017-01-11 | 新疆玉昆仑天然食品工程有限公司 | Donkey milk brassica campestris powder and preparation method thereof |
| CN107151638A (en) * | 2017-05-25 | 2017-09-12 | 中驭(北京)生物工程有限公司 | One plant improvement liver function Lactobacillus plantarum ZY001 and its application in acidified milk |
| CN111449139A (en) * | 2020-04-08 | 2020-07-28 | 杭州联星贸易有限公司 | Formula donkey milk powder containing N-acetylneuraminic acid |
| CN111493148A (en) * | 2020-06-01 | 2020-08-07 | 西安小天使生命营养科学健康研究院有限公司 | Donkey milk product and preparation process thereof |
| CN114271335A (en) * | 2021-11-30 | 2022-04-05 | 倍恩喜(湖南)乳业有限公司 | Dairy product and method for producing the same |
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