CN107151638A - One plant improvement liver function Lactobacillus plantarum ZY001 and its application in acidified milk - Google Patents
One plant improvement liver function Lactobacillus plantarum ZY001 and its application in acidified milk Download PDFInfo
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- CN107151638A CN107151638A CN201710376089.5A CN201710376089A CN107151638A CN 107151638 A CN107151638 A CN 107151638A CN 201710376089 A CN201710376089 A CN 201710376089A CN 107151638 A CN107151638 A CN 107151638A
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/123—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
- A23C9/1234—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt characterised by using a Lactobacillus sp. other than Lactobacillus Bulgaricus, including Bificlobacterium sp.
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/123—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
- A23C9/1238—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt using specific L. bulgaricus or S. thermophilus microorganisms; using entrapped or encapsulated yoghurt bacteria; Physical or chemical treatment of L. bulgaricus or S. thermophilus cultures; Fermentation only with L. bulgaricus or only with S. thermophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/175—Rhamnosus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
- A23V2400/249—Thermophilus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
- C12R2001/25—Lactobacillus plantarum
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Dairy Products (AREA)
Abstract
The invention discloses one plant of Lactobacillus plantarum (lactobacillus plantarum, L.plantarum) ZY001 for improving liver function and its application in acidified milk, belong to microbial technology field.The isolated Lactobacillus plantarum ZY001 from the traditional donkey Yoghourt in Xinjiang, is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, culture presevation CGMCC 13505.Using Lactobacillus plantarum ZY001, it is used alone or with Lactobacillus rhamnosus, streptococcus thermophilus with 13: 12: 1 proportioning, with donkey milk: milk=2 4: 86 are that fermenting raw materials prepare donkey milk acidified milk.Lactobacillus plantarum ZY001 disclosed by the invention possesses the effect for improving liver function.
Description
Technical field
The invention belongs to microbial technology field.More particularly it relates to the newborn bar of the plant of one plant of improvement liver function
Bacterium (lactobacillus plantarum, L.plantarum) ZY001 and its application in acidified milk.
Background technology
Kashi Area of Xingjiang Autonomous Region has superior grass resource condition and the abundant stalk resource of planting industry, possesses donkey kind, number
Resources advantage is measured, is that donkey industry development creates advantageous condition.2008, Xinjiang Donkey was listed in national Application of Animal Genetic
Protection of resources kind.
Donkey milk is as one of principal by product of donkey aquaculture, not only with higher nutritive value, and with wider
General medical value.Other milk supplies are compared, and donkey milk is the day of a kind of low fat, low cholesterol, high calcium, selenium-rich, beauty, anti-aging
The closest human milk of ratio of each nutritional ingredient in right dairy products, donkey milk.Contain substantial amounts of epithelical cell growth factor in donkey milk
(EGF), EGF can strengthen the immunity of human body, resistance；Epithelial cell Nutrition and Metabolism, protection skin and prevention skin can be promoted
Skin has promotion subcutaneous collagen cytothesis function due to being lost caused by a variety of causes.
Domestic and international clinical research shows that the reversible intestinal flora of probiotic lactobacillus is unbalance, when intestinal submucosal tissue is impaired tight
Weight, special dietary material of the increase rich in reparation enterocyte, such as polysaccharide prebiotics, integration of drinking and medicinal herbs function ingredients etc., it is right
Recovering function of intestinal canal has definite gain effect, can reduce enterogenic infection and the incidence of organ failure, is to reverse disease
The key link of acute turn of danger of disease, so as to reduce case fatality rate；Use and the hospital stays of antibiotic can be reduced simultaneously, reduction medical treatment
Expense, Tiny ecosystem nutrition treatment opens one for disease treatment and fans new window.
Lactobacillus plantarum is one kind of lactic acid bacteria, is normally present in the vegetables and fruit juice of fermentation, food, ensilage and
The fields such as health care are received more and more attention.The bacterial strain belongs to homofermentative lactic bacteria, can utilize glucose, malt
Sugar, sucrose, L- sorboses, lactose, cellobiose, xylose, melibiose, fructose, ribose, sodium gluconate production acid, but fermentation Portugal
Grape sugar and sodium gluconate not aerogenesis.During using strain fermentation lactose production acid treatment Lactose in Whey conversion, with other
The incomparable superiority of strain, it both can be in high conversion rate glucose using Lactose conversion, can utilize again in whey
Residual protein.
The present invention characteristic be：1st, strain source is isolated from the local method donkey Yoghourt of Xinjiang in Kashi Area of Xingjiang Autonomous Region
Lactic acid bacteria；2nd, Lactobacillus plantarum, with intestinal flora balance is maintained, improves body and exempted from as the beneficial flora in human body intestinal canal
A variety of functions such as epidemic disease power and improvement liver function；3rd, the products scheme that the A of CN 104770469 are provided is with pure donkey milk or adds other
The donkey milk of the natural extracts aqueous solution of casein containing protein is not that fermenting raw materials produce sour milk product, wherein due to donkey milk junket egg
The reason for Bai Hanliang is low can not realize the preparation of Yoghourt, can be classified as one kind of lactic acid drink.The present invention Yoghourt preparation process be
Fermentation kind is prepared with 1~3: 1~2: 1 proportioning with Lactobacillus plantarum or Lactobacillus plantarum, Lactobacillus rhamnosus, streptococcus thermophilus
Son, is seeded to donkey milk: milk=2~4: in 8~6 mixing dairy milk starting material, fermentation obtains curds type donkey milk fermented dairy product.
The content of the invention
[technical problem to be solved]
It is an object of the invention to provide one plant improvement liver function Lactobacillus plantarum (lactobacillus plantarum,
L.plantarum)ZY001。
It is a further object to provide applications of the described Lactobacillus plantarum ZY001 in acidified milk.
[technical scheme]
The present invention is achieved through the following technical solutions.
The present invention relates to a lactobacillus plantarum ZY001, the bacterial strain is on 09 02nd, 2016 in Chaoyang District, Beijing City
No. 3 China Committee for Culture Collection of Microorganisms's common micro-organisms center's preservations of the institute of North Star West Road 1, preserving number is respectively
CGMCC 13505。
A preferred embodiment of the invention, described newborn class fermented product is acidified milk.
It is prepared by a preferred embodiment of the invention, Lactobacillus plantarum ZY001 and its application in acidified milk
Step is as follows：
(1) the isolated lactic acid bacteria ZY001 from the local method donkey Yoghourt of Xinjiang, identified is Lactobacillus plantarum, i.e. plant breast
Bacillus ZY001；
(2) with donkey milk and milk mixing breast for raw material, inoculated plant lactobacillus ZY001 fermentations obtain the fermentation of single bacterium donkey milk
Breast；
(3) with single bacterium donkey milk acidified milk gavage alcoholic hepatic injury SD rats, glutamic-oxalacetic transaminease (AST) and third turn of ammonia of paddy are determined
Enzyme (ALT) content, determines that Lactobacillus plantarum ZY001 improves effect of liver function；
(4) Lactobacillus plantarum ZY001, Lactobacillus rhamnosus, streptococcus thermophilus are prepared into fermentation with 1~3: 1~2: 1 proportioning
Seed, is seeded to donkey milk: milk=2~4: in 8~6 raw material, fermentation obtains donkey milk fermented dairy product.
Fermentation temperature described in step (2) is 42 DEG C, and fermentation termination is pH 4.0, i.e. curdled milk time；
Animal model every group 10, respectively blank group, saline control group, Lactobacillus plantarum described in step (3)
ZY001 acidified milks experimental group and the newborn group of mixing；Mode is given for gavage；Biochemical indicator blood is determined with automatic blood Biochemical Analyzer
The activity of glutamic-oxalacetic transaminease (AST) and glutamic-pyruvic transaminase (ALT) in clear.
The implementation of the present invention program, at least has the advantage that：
According to the inventive method, fermented bacterium is located away from the native bacterium fermentation donkey of the relatively low Kashi Area of Xingjiang Autonomous Region of the hepatopathy incidence of disease
Milk, animal experiment proves that, the bacterium has the liver function effect that has some improvement.One of application main material of the present invention is donkey milk,
Donkey milk has a strengthen immunity, beauty, effect of anti-aging, after being fermented through Lactobacillus plantarum ZY001, be more easy to be absorbed by the body,
Metabolism, fermented product possesses donkey milk and Lactobacillus plantarum ZY001 double effects.From this hair unlike the A of CN 106190930
The strain of bright offer is the strain for possessing independent intellectual property right voluntarily separated, has carried out Patent Deposit；With CN 104770469
A is compared, and the product that the present invention is provided is fermented milk products truly, in curdy.
Embodiment
Below in conjunction with specific embodiment, further the present invention is described in detail, so that the purpose of the present invention, technology
Scheme and advantage are clearer.Following examples are only used for for example, not constituting any limitation to the present invention.
Embodiment 1
Lactobacillus plantarum ZY001 be separated from the herdsman family natural fermented sourdough donkey milk of Xinjiang, filter out one plant have
The Lactobacillus plantarum of excellent fermentation character.By ZY001 streak inoculations to MRS agar mediums, 42 DEG C of culture 24h, in the condition
The lower generation of progress continuous passage culture 25, unknown significance variation.
Picking ZY001 bacterium colonies, setting-out is inoculated on MRS fluid nutrient mediums, is cultivated under conditions of 37 DEG C of temperature and anaerobism
48h；After bacterium colony is formed, it is inoculated with 1% inoculum concentration under the conditions of MRS fluid nutrient mediums, 37 DEG C and expands culture 24h；Draw again
Line is preserved on MRS agar mediums, confirming as puncturing after pure culture on MRS semisolid culturemediums at 4 DEG C.Observation
Colonial morphology, the Gram's staining cell morphological characteristic of pure culture are recorded, and carries out conventional catalase and oxidizing ferment experiment.
It is in that shaft-like bacterial strain temporary designations are breast by Gram-positive, catalase and oxidase test feminine gender and cellular morphology
Bacillus.The bacterial strain forms lurid circular colonies projection on MRS culture mediums, and surface is smooth, fine and closely woven, observes under the microscope
Cell be generally elongated rod-shaped.
The MRS agar mediums that the present invention is used close sulphur by 10g peptones, 10g beef extracts, 5g yeast extracts, 2g citric acids
The blue acetate hydrate of sour magnesium, 20g glucose, 1ml Tween 80s, 5g is received, the hypophosphite monohydrate hydrogen dipotassiums of 2g tri-, 0.58g bitter salts,
0.25g Manganous sulfate monohydrates, 6g agar and 1000ml distilled water are constituted, its pH 6.2-6.6.
The MRS fluid nutrient mediums that the present invention is used are by 10g peptones, 10g beef extracts, 5g yeast extracts, 2g hydrogen citrates
Diamines, 20g glucose, 1ml Tween 80s, the acetate hydrates of 5g tri- are received, the hypophosphite monohydrate hydrogen dipotassiums of 2g tri-, 0.58g bitter salts,
0.25g Manganous sulfate monohydrates are constituted with 1000ml distilled water, its pH 6.2-6.6.
In terms of MRS fluid nutrient medium weight 2% inoculum concentration, training is expanded by the strain isolated and purified under the conditions of 37 DEG C
Support, carbohydrate production sour (API50CH) is then carried out to microbial strain culture reacts, and identifies the physicochemical property of strain.
With kit extract strain DNA, design 16S rDNA and pheS gene orders primer go forward side by side performing PCR reaction and survey
Sequence.Compared by 16S rDNA and pheS gene homology, the bacterial strain and Lactobacillus plantarum
WCFS1(Sequence ID：NC_004567.2) there is 99% homology.By the comprehensive analysis to multinomial experimental data, mirror
Bacterial strain ZY001 is determined for Lactobacillus plantarum.
Table 1 is strain ZY001 cellular morphologies and Physiology and biochemistry qualification result.
Embodiment 2：
The Lactobacillus plantarum ZY001 of activation is inoculated in MRS fluid nutrient mediums, 37 DEG C of culture 24h are connect with 4% inoculum concentration
Plain chocolate is planted, 42 DEG C of quiescent cultures are to curdled milk, after being sufficiently stirred for, and take caseous fermentation liquid to be seeded to donkey milk with 4% inoculum concentration: milk
In=3: 7 mixing breast, 42 DEG C of cultures obtain Lactobacillus plantarum ZY001 acidified milks, the curdled milk time is 12h, during curdled milk to curdled milk
PH is 4.0.Obtaining the unfermentable mixing breast of Lactobacillus plantarum ZY001 acidified milks and configuration is used for follow-up zoopery.
Embodiment 3：Rat builds alcoholic liver injury model
40 SD rats (male and female half and half) machines are divided into model group and blank group, body weight 180-200g, in temperature 22-24
DEG C, humidity 30-40%, illumination and interlunation, Normal group was given as model group animal to be raised in 12h environment
Basal diet, model group presses the ethanol solution 12mL/kg*bw of the dosage gavage 50% of 0.2mL/100g weights, 1 time a day；
Normal group gives isometric distilled water, continues after 30 days, is determined with automatic blood Biochemical Analyzer in biochemical indicator serum
The activity of glutamic-oxalacetic transaminease (AST) and glutamic-pyruvic transaminase (ALT).Successful SD rats will be modeled to be grouped at random, every group 10, point
Wei not blank group, saline control group, Lactobacillus plantarum ZY001 acidified milks experimental group and the newborn group of mixing；It is filling to give mode
Stomach, gavage amount 5mL/ times, 2 times a day, and glutamic-oxalacetic transaminease in biochemical indicator serum is determined with automatic blood Biochemical Analyzer after 2 weeks
(AST) and glutamic-pyruvic transaminase (ALT) activity, the results are shown in Table 2.Found through zoopery, with single bacterium donkey milk acidified milk gavage second
Alcohol hepatic injury SD rats, can significantly improve AST and ALT expression, it can be seen that, Lactobacillus plantarum ZY001 possesses improvement liver function
Effect of energy.
Embodiment 4：The preparation of donkey milk acidified milk
With donkey milk and milk mixing breast for raw material, substrate proportioning 2: 8,3: 7,4: 6.Activation culture company early stage is preserved respectively
Three kinds of bacterium are cultivated 24h for 37 DEG C by Lactobacillus rhamnosus, streptococcus thermophilus and Lactobacillus plantarum ZY001 in MRS culture mediums
Afterwards, 4% it is inoculated in after sterile plain chocolate, 42 DEG C of quiescent culture to curdled milks by Lactobacillus plantarum ZY001, Lactobacillus rhamnosus and thermophilic
Hot streptococcus=1: 1: 1,1: 2: 1,2: 1: 1,2: 2: 1；3: 1: 1,3: 2: 1 various proportioning inoculation donkey milk milk mixing breasts (2-4:
8-6), 42 DEG C of quiescent cultures obtain the donkey milk fermented dairy product of different ratio to curdled milk.
The strain ZY001 cellular morphologies of table 1 and physical and chemical experiment result table
Note：+ represent negative reaction；
- represent negative reaction.
Fig. 1 strain ZY001 16SrDNA gene order sequencing results
ATACATGCAGTCGAACGAACTCTGGTATTGATTGGTGCTTGCATCATGATTTACATTTGAGTGAGTGGC
GAACTGGTGAGTAACACGTGGGAAACCTGCCCAGAAGCGGGGGATAACACCTGGAAACAGATGCTAATACCGCATAA
CAACTTGGACCGCATGGTCCGAGCTTGAAAGATGGCTTCGGCTATCACTTTTGGATGGTCCCGCGGCGTATTAGCTA
GATGGTGGGGTAACGGCTCACCATGGCAATGATACGTAGCCGACCTGAGAGGGTAATCGGCCACATTGGGACTGAGA
CACGGCCCAAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCACAATGGACGAAAGTCTGATGGAGCAACGCCGCG
TGAGTGAAGAAGGGTTTCGGCTCGTAAAACTCTGTTGTTAAAGAAGAACATATCTGAGAGTAACTGTTCAGGTATTG
ACGGTATTTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGG
ATTTATTGGGCGTAAAGCGAGCGCAGGCGGTTTTTTAAGTCTGATGTGAAAGCCTTCGGCTCAACCGAAGAAGTGCA
TCGGAAACTGGGAAACTTGAGTGCAGAAGAGGACAGTGGAACTCCATGTGTAGCGGTGAAATGCGTAGATATATGGA
AGAACACCAGTGGCGAAGGCGGCTGTCTGGTCTGTAACTGACGCTGAGGCTCGAAAGTATGGGTAGCAAACAGGATT
AGATACCCTGGTAGTCCATACCGTAAACGATGAATGCTAAGTGTTGGAGGGTTTCCGCCCTTCAGTGCTGCAGCTAA
CGCATTAAGCATTCCGCCTGGGGAGTACGGCCGCAAGGCTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGG
TGGAGCATGTGGTTTAATTCGAAGCTACGCGAAGAACCTTACCAGGTCTTGACATACTATGCAAATCTAAGAGATTA
GACGTTCCCTTCGGGGACATGGATACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTC
CCGCAACGAGCGCAACCCTTATTATCAGTTGCCAGCATTAAGTTGGGCACTCTGGTGAGACTGCCGGTGACAAACCG
GAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGATGGTACAA
CGAGTTGCGAACTCGCGAGAGTAAGCTAATCTCTTAAAGCCATTCTCAGTTCGGATTGTAGGCTGCAACTCGCCTAC
ATGAAGTCGGAATCGCTAGTTATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCG
TCACACCATGAGAGTTTGTAACACCCAAAGTCGGTGGGGTAACCTTTTAGGAACCAGCCGCCTAAGGTGGGACAGAT
GATTAGGGTGAAGTCGTAACAAG
Fig. 2 strain ZY001 pheS gene order sequencing results
AAGACGTGCTACTACGCACGCAGACGTCTGCTGATCAGCCGCGGTCACTTGAAAATCACGATTTTTCTA
AAGGACCGCTGAAGGTCTTGTCACCTGGCCGCGTTTATCGGCGTGATACGGATGATGCAACCCATTCCCATCAATTT
CATCAAATTGAAGGGTTAGTCGTGGACAAGCATATTACGATGGCTGATTTGAAGGGCACCTTAATTCTGGTTGCCAA
GACTTTGTTTGGCGATCAATTCGAGTTTCGGCTACGGCCAAGCTTCTTTCCATTCACGGAACCATCCGTAGAAGCTG
ATGTAACTTGCTTTAATTGCAATGGCAAGGGCTGTGCAATCTCTAAGCAAACGGGTTGGATCGAAGTACTGGGTGCC
GGCATGGTTCACCCCCACGTGTTAGAAATGTCTGGCATTGATCCAGAAGAATATGGTGGCTTTGCTTTCGGGCCTAG
G
Enzyme index of the acidified milk of table 2 to alcoholic liver injury in rats model
| Group | AST(U/L) | ALT(U/L) |
| Normal group | 19.82±4.32 | 26.21±4.38 |
| Model group | 51.21±6.38 | 46.89±6.34 |
| Saline control group | 50.76±4.75 | 45.86±5.56 |
| The newborn group of mixing | 45.25±6.78 | 41.77±7.18 |
| Lactobacillus plantarum ZY001 acidified milk experimental groups | 36.53±6.75 | 36.45±6.17 |
Claims (6)
1. the Lactobacillus plantarum ZY001 (Lactobacillus plantarum, L.plantarum) of one plant of improvement liver function
ZY001, the bacterial strain is on 09 02nd, 2016 in Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Chinese Academy of Sciences microorganism
China Committee for Culture Collection of Microorganisms's common micro-organisms center of research institute preservation, its preserving number is CGMCC 13505.
2. Lactobacillus plantarum ZY001 according to claim 1, it is characterised in that the bacterium is located away from the new of region feature
The native bacterium of boundary donkey milk fermentation.
3. Lactobacillus plantarum ZY001 according to claim 1, it is characterised in that the bacterium can be used alone for fresh milk especially
It is the fermentation of donkey milk；Or include Lactobacillus rhamnosus, streptococcus thermophilus, Lactobacillus delbrueckii Asia with other lactic acid bacterias
Kind, lactobacillus paracasei, bifidobacterium longum etc. merge using the double bacterium of progress or many cooperative fermentation fresh milks especially donkey milk.
4. purposes according to claim 2, it is characterised in that the preparation method of the acidified milk is as follows：
(1) strain of preservation is inoculated in commercialization lactic acid bacteria culturing medium MRS inclined-planes, 37-38 DEG C of culture 24h by actication of culture；With
MRS fluid nutrient mediums, 37-38 DEG C of culture 24h are inoculated with afterwards；
(2) prepared by seed：140g/L skimmed milk powers are configured as seed fermentation culture medium, 115 DEG C of 15min that sterilize, after cooling, with
2-4% inoculum concentrations are inoculated with, 41-43 DEG C of culture 12h；
(3) ferment：By Lactobacillus plantarum ZY001 with 4% inoculum concentration, donkey milk is inoculated into: in milk=2-4: 8-6 raw material, 42
DEG C culture is to curdled milk state, pH3.8；Or by Lactobacillus plantarum ZY001, Lactobacillus rhamnosus, streptococcus thermophilus with 1-3: 1-2: 1
Proportioning prepares fermentation seed, and total inoculum concentration 3-6% is inoculated into donkey milk: in milk=1-2: 4-3 raw material, 42 DEG C are cultivated to solidifying
Emulsus state.
5. Lactobacillus plantarum ZY001 according to claim 1, it is characterised in that the bacterium possesses the effect for improving liver function.
6. fresh milk according to claim 2 includes fresh milk, mare's milk, goat milk, camel milk, donkey milk.
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