CN111543639A - Food composition and pharmaceutical composition containing lactic acid bacteria strain for protecting liver - Google Patents
Food composition and pharmaceutical composition containing lactic acid bacteria strain for protecting liver Download PDFInfo
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- CN111543639A CN111543639A CN201910110144.5A CN201910110144A CN111543639A CN 111543639 A CN111543639 A CN 111543639A CN 201910110144 A CN201910110144 A CN 201910110144A CN 111543639 A CN111543639 A CN 111543639A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The present invention provides food compositions and pharmaceutical compositions containing lactic acid bacteria strains for protecting the liver. The present invention provides an isolated lactic acid bacterial strain selected from at least one of the following: lactobacillus plantarum TSP05 strain, Lactobacillus fermentum TSF331 strain, and Lactobacillus reuteri TSR332 strain. The lactobacillus strain has liver protecting function, and is in the form of food composition or pharmaceutical composition.
Description
Technical Field
The present invention relates to a food composition and a pharmaceutical composition, and more particularly to a food composition and a pharmaceutical composition comprising a lactic acid bacterial strain having a hepatoprotective function.
Background
The liver is the largest organ of the human body and is responsible for very important tasks including: 1. the circulation function is as follows: bringing blood from the portal artery to the systemic circulation, the reticuloendothelial system (reticuloendothelial system) of the blood is involved in immune mechanism and stores the blood, and has the function of regulating blood volume; 2. the excretion function is as follows: bile is formed and discharged into the intestine, combined with bile pigments (bilirubin conjugates) secreted by the liver, cholesterol (cholestrol), cholic acid (cholic acid) to form bile salts, and substances separated from the blood by the liver cells, such as heavy metals, dyes (brolsulein), alkaline phosphatase (alkalophatase), and the like, can be excluded; 3. metabolic function: participate in the metabolism of carbohydrates, proteins, fats, minerals and vitamins, and produce heat; 4. the defending function and the detoxifying function: the Kupffer cell (Kupffer cell) can remove foreign matters in blood through phagocytosis, reduce the toxicity of noxious substances through combination, methylation, oxidation and reduction, and then is taken out of a body through an excretion system; also has the function of removing ammonia from blood, in particular ammonia molecules absorbed via portal vein; and 5. hematopoietic and blood clotting functions: the liver has hematopoietic function in embryonic stage, and also in abnormal cases after adult life, and additionally, blood coagulation substances such as fibrinogen, prothrombin and heparin, and destruction of old red blood cells can be produced. Therefore, it is very important for the human body to maintain good liver function.
Ethanol (alcohol) is an important cause of hepatitis. Alcoholic hepatitis is generally caused after long-term alcoholism. Symptoms of alcoholic hepatitis include discomfort, enlarged liver, ascites tumor, and high liver function index value. Milder alcoholic hepatitis only has elevated liver function index values. The severe symptoms include severe liver inflammation, jaundice, increased prothrombin time, and liver failure. In the most severe cases, the patient may be confused, accompanied by an increase in the bilirubin level and prolonged prothrombin time. In these severe cases, mortality rates of 50% occur within 30 days after symptoms appear.
The anatomical appearance of the liver in non-alcoholic steatohepatitis is very similar to that of alcoholic hepatitis (fat droplets, inflamed cells), but these patients have no history of alcohol abuse. One relatively mild and similar disease is fatty liver disease (steatoshiftis), abbreviated as "fatty liver". Fatty liver is also found in 80% of patients with obesity. The anatomy of the liver of these patients can see fat droplets everywhere in the liver, but there is no sign of inflammation. Fatty liver may further develop into hepatitis, cirrhosis, and even hepatocellular carcinoma.
A few strains of lactic acid bacteria have been found to have liver-protecting activity through experimental procedures and have been confirmed. It is emphasized that the function of lactic acid bacteria on physical health lies in the specificity of the strains (strains) rather than the species (species), and that such strains with a particular effect on human health are called functional probiotics (microorganisms for the evaluation of probiotics in foods; Report of joint FAO/WHO working group on drying peptides for the evaluation of probiotics in foods; London Ontario, Canada April 30and May 1,2002: 1-7).
In summary, there is an urgent need to develop a nutritional supplement that is safe and can be used for a long time to protect the liver. Since lactic acid bacteria are generally safe, it is a very important objective to find out a lactic acid bacteria strain having liver protecting function.
Disclosure of Invention
The present invention provides a food composition and a pharmaceutical composition containing a lactic acid bacterium strain, which can protect the liver and reduce liver inflammation and fatty liver.
The food composition containing lactobacillus strains according to an embodiment of the present invention comprises lactobacillus strains having liver protecting activity, wherein the lactobacillus strains are selected from at least one of the following isolated lactobacillus strains: lactobacillus plantarum TSP05 strain with preservation number of CGMCC No. 16710; lactobacillus fermentum TSF331 strain with preservation number of CGMCC No. 15527; and Lactobacillus reuteri TSR332 with the preservation number of CGMCC No.15528, wherein the Lactobacillus strains are all preserved in the common microorganism center of China Committee for culture Collection of microorganisms; and a physiologically acceptable excipient, diluent or carrier.
In another embodiment of the present invention, the pharmaceutical composition comprises a lactic acid bacterial strain having an effect of protecting liver activity, wherein the lactic acid bacterial strain is selected from at least one isolated lactic acid bacterial strain selected from the group consisting of: lactobacillus plantarum TSP05 strain with preservation number of CGMCC No. 16710; lactobacillus fermentum TSF331 strain with preservation number of CGMCC No. 15527; and Lactobacillus reuteri TSR332 with the preservation number of CGMCC No.15528, wherein the Lactobacillus strains are all preserved in the common microorganism center of China Committee for culture Collection of microorganisms; and a pharmaceutically acceptable excipient, diluent or carrier.
The purpose, technical content, features and effects of the present invention will be more readily understood by the following detailed description of the embodiments taken in conjunction with the accompanying drawings.
Drawings
FIG. 1 is a graph showing the results of animal experiments with alanine Aminotransferase (ALT) in the serum of groups of mice fed with the lactic acid bacteria strain of the present invention.
FIG. 2 is a graph showing the results of animal experiments on glutamic-oxaloacetic transaminase (AST) in the serum of various groups of mice fed with the lactic acid bacterium strain of the present invention.
FIG. 3 shows the results of animal experiments with triglycerides in the serum of various groups of mice fed with the lactic acid bacteria strain of the present invention.
FIG. 4 is the results of animal experiments on total cholesterol in serum of various groups of mice fed with the lactic acid bacterium strain of the present invention.
FIG. 5 is a graph showing the results of animal experiments on triglycerides in the livers of groups of mice fed with the lactic acid bacterium strain of the present invention.
FIG. 6 is the results of animal experiments with glutathione in the livers of groups of mice fed with the lactic acid bacterium strain of the present invention.
FIG. 7 is a graph showing the results of animal experiments on glutathione peroxidase activity in the livers of groups of mice fed with the lactic acid bacterium strain of the present invention.
Microbial preservation for patent procedure:
TSP05
The preservation date is as follows: 11/2018, 05/month;
the preservation unit: china general microbiological culture Collection center (CGMCC);
the address of the depository: xilu No.1 Hospital No. 3, the institute of microbiology, China academy of sciences, Beijing, Chaoyang
The preservation number is: CGMCC No. 16710;
and (3) classification and naming: lactobacillus plantarum (Lactobacillus plantarum);
(II) TSF331
The preservation date is as follows: 29 months 03, 2018;
the preservation unit: china general microbiological culture Collection center (CGMCC);
the address of the depository: xilu No.1 Hospital No. 3, the institute of microbiology, China academy of sciences, Beijing, Chaoyang
The preservation number is: CGMCC No. 15527;
and (3) classification and naming: lactobacillus fermentum;
(III) TSR332
The preservation date is as follows: 29 months 03, 2018;
the preservation unit: china general microbiological culture Collection center (CGMCC);
the address of the depository: xilu No.1 Hospital No. 3, the institute of microbiology, China academy of sciences, Beijing, Chaoyang
The preservation number is: CGMCC No. 15528;
and (3) classification and naming: lactobacillus reuteri.
Detailed Description
The following detailed description of the various embodiments of the invention, taken in conjunction with the accompanying drawings, is provided by way of illustration. Aside from the detailed description, this invention is capable of broad application in other embodiments and many variations and modifications of the invention will be apparent to those skilled in the art upon reading the specification and understanding the present invention. In the description of the specification, numerous specific details are set forth in order to provide a more thorough understanding of the invention; however, the present invention may be practiced without some or all of these specific details. In other instances, well-known steps or elements have not been described in detail so as not to unnecessarily obscure the present invention. The same or similar elements in the drawings will be denoted by the same or similar symbols. It is noted that the drawings are merely schematic and do not represent actual sizes or quantities of elements, and some details may not be fully drawn for clarity of drawing.
The freeze-dried culture of the lactobacillus strain is preserved in the common microorganism center of China Committee for culture Collection of microorganisms (CGMCC, institute No. 3 of Microbiol No.1 of Naja district, Beijing, China academy of sciences, 100101). The details of the deposit are shown in table 1:
TABLE 1 deposited data of Lactobacillus strains
Name of Strain | Classification | Deposit number | Date of storage |
TSP05 | Lactobacillus plantarum | CGMCC No.16710 | 11/2018/11/05 |
TSF331 | Lactobacillus fermentum | CGMCC No.15527 | 29/03/2018 |
TSR332 | Lactobacillus reuteri | CGMCC No.15528 | 29/03/2018 |
Of the three deposited lactic acid bacteria listed in Table 1, Lactobacillus plantarum (Lactobacillus plantarum) TSP05 strain, Lactobacillus fermentum (Lactobacillus fermentum) TSF331 strain, and Lactobacillus reuteri (Lactobacillus reuteri) TSR332 strain were found to have the benefit of protecting the liver from liver inflammation.
The present invention comprises a lactic acid bacterial strain having an effect of protecting liver activity, the lactic acid bacterial strain being selected from at least one of the following isolated lactic acid bacterial strains: lactobacillus plantarum TSP05 strain with preservation number of CGMCC No. 16710; lactobacillus fermentum TSF331 strain with preservation number of CGMCC No. 15527; and Lactobacillus reuteri TSR332 with the preservation number of CGMCC No.15528, wherein the Lactobacillus strains are all preserved in China general microbiological culture Collection center (CGMCC); and a physiologically acceptable excipient, diluent or carrier, or a pharmaceutical composition comprising a pharmaceutically acceptable excipient, diluent or carrier. Wherein the lactic acid bacteria strain is active strain.
In the case of food compositions, the physiologically acceptable excipient, diluent or carrier may be a food product. For example, the food product may include, but is not limited to, a dairy drink, tea, coffee, chewing gum, dentrifices (e.g., buccal tablets, chewable lozenges, soft candies, etc.), or combinations thereof, wherein the dairy drink may include fermented milk, yogurt, cheese, or dairy drink milk powder, and the like. The pharmaceutical composition may be in an oral dosage form. For example, oral dosage forms can be tablets, capsules, solutions, powders, and the like.
In the case of food or pharmaceutical compositions, the lactic acid bacterial strain is present in an amount of 106Above CFU; preferably, the number of lactic acid bacteria strains is 1010Above CFU.
It is noted that the literature indicates that not all strains of lactic acid bacteria of the same species are helpful for protecting the liver. For example, Xingrong Zhao et al (PLoS ONE 7(2): e30696.doi:10.1371/journal. po.0030696) used a high fat diet to induce an obese mouse model to investigate the efficacy of lactic acid bacterial strains on high fat diet induced obesity and fatty liver. The test results showed that only Pediococcus pentosaceus (Pediococcus pentosaceus) strain LP28 could reduce weight gain and triglyceride and cholesterol in the liver, but that lactobacillus plantarum (lactobacillus plantarum) SN13T strain and the heat-killed LP28 strain were not effective. Alternatively, Yi Qiao et al (Journal of functional foods 14(2015) 424) 434) studied the effect of different L3 and L10 strains of Lactobacillus reuteri on inflammation and fat storage in a mouse model of obesity on high fat diet. The test results showed that only the L3 strain had the effect of improving liver hypertrophy and liver steatosis, while the L10 strain had no effect. Therefore, the bacterial strains claimed by the invention only comprise Lactobacillus plantarum TSP05 (with the preservation number of CGMCCNo.16710) and Lactobacillus fermentum TSF331 (with the preservation number of CGMCCNo.15527) which are preserved in China general microbiological culture Collection center (CGMCC); and Lactobacillus reuteri TSR332 (accession number cgmccno.15528), not widely including all the same species of lactic acid bacteria strains.
Example 1: morphological and general Properties of Lactobacillus strains with hepatoprotective function
The taxonomical characteristics of the strain were confirmed based on the results of 16S rDNA sequence analysis and analysis by the API bacterial identification system. The morphological and general characteristics of the above strains are detailed in table 2:
table 2 morphological and general characterization of the lactic acid bacteria strains of the invention
Example 2: collection, culture and preservation of lactic acid bacteria strains
The lactic acid bacterial strains collected by the applicant were stored at-80 ℃ with 20% glycerol. Before use, the mixture was activated twice (24 hours) at 37 ℃ with MRS broth (DIFCO) containing 0.05% cysteine. Lactobacillus strains used for research, including Lactobacillus plantarum (Lactobacillus plantarum) TSP05 strain, Lactobacillus fermentum (Lactobacillus fermentum) TSF331 strain, and Lactobacillus reuteri (Lactobacillus reuteri) TSR332 strain, wherein the source of Lactobacillus plantarum (Lactobacillus plantarum) TSP05 strain is kimchi; the sources of the Lactobacillus fermentum TSF331 strain and the Lactobacillus reuteri TSR332 strain were the intestinal tracts of healthy chickens.
Example 3: animal test of lactic acid bacteria strain with liver protecting function
Under the condition of alcohol diet, C57BL/6N mice are used as a research mode to evaluate the influence of the lactobacillus strains on the oxidative damage of liver tissues of the mice induced by the alcohol, and liver function indexes such as alanine Aminotransferase (ALT) and aspartate Aminotransferase (AST) in serum and the relation between the content of triglyceride in the liver and fatty liver are measured. Aspartate Aminotransferase (AST) is also present in the liver, myocardium, muscle, and within erythrocytes; glutamate pyruvate transaminase (ALT) is mainly present in hepatocytes. When these cells are necrotized and destroyed by various causes, intracellular transaminase is released into blood, so that there is a possibility that AST value is increased in hepatitis, myocardial infarction, muscle inflammation or hemolysis, and the degree of cell destruction can be estimated by examining the increase of these enzymes by blood drawing. Similarly, elevated ALT levels are the result of liver inflammation. For the liver, when the liver cells are damaged by drugs, alcohol, virus, hypoxia, etc., the damaged condition of liver cells can be known by examining the AST value or ALT value rise through blood drawing test.
First, 44C 57BL/6N male mice were divided into six groups, namely, a control group (control group), an alcohol group (alcohol), an alcohol plus TSP05 strain group (alcohol + TSP05), an alcohol plus TSF331 and TSR332 strain group (alcohol + TSF331+ TSR332), an alcohol plus TSP05, a TSF331 and TSR332 strain group (alcohol + Mix-LAB), and a TSP05, TSF331 and TSR332 strain group (Mix-LAB). The control group and TSP05, TSF331 and TSR332 strain groups were fed freely on Lieber-DeCarli fluid normal feed, and the alcohol group and alcohol plus lactic acid bacteria strain groups were fed freely on Lieber-DeCarli fluid alcohol feed. The control group and the alcohol group were fed with secondary water through a gastric tube once a day, and the TSP05, TSF331 and TSR332 strains and the alcohol plus lactic acid bacteria strains were fed with the lactic acid bacteria strain samples for four weeks. During the experiment, blood was collected every two weeks and analyzed for alanine Aminotransferase (ALT), aspartate Aminotransferase (AST), triglyceride and total cholesterol, and mouse liver tissue was collected after sacrifice at week 4 for liver triglyceride, Glutathione (Glutathione) content and Glutathione peroxidase (Glutathione peroxidase) analysis.
Glutathione is an important antioxidant substance in the human body, and is often called "antioxidant mother", but gradually decreases with the influence of aging, irregular life, bad eating habits, and the like. The concentration of glutathione in the liver may indicate its antioxidant capacity. Glutathione peroxidase is a general term for an enzyme family with peroxidase activity, and the main biological effect of the glutathione peroxidase is to play a role in detoxification in organisms and prevent the organisms from being damaged by oxidation. Glutathione peroxidase is a enzyme that reduces lipid peroxides to the corresponding alcohol and reduces free hydrogen peroxide to water, while catalyzing the conversion of glutathione to the oxidized form.
Glutathione (Glutathione, GSH) concentration assay:
according to the QuantiChromTM glutamthione Assay Kit operating manual. Adding phosphate buffer solution into liver tissue at a ratio of 1:4, homogenizing, collecting 200 μ L of homogenized solution, centrifuging at 4 deg.C at 14,000rpm for 10 min, and collecting supernatant. Then configure Blank and calibration: mu.L (ddH)2O and 100. mu.L Calibrator were added to a 96-well plate, followed by 200. mu.L ddH2O addition of Blank andmixing in Calibrator well. mu.L of the LSample was then mixed with 120. mu.L of Reagent A in a 1.5mL centrifuge tube and centrifuged at 14,000 min for 5 min if turbidity was present. Next, 200. mu.L of the Sample/Reagent A mixture was added to the 96-well plate, 100. mu.L of the Reagent B was added thereto and mixed well by tapping the well plate, and after standing at room temperature for 25 minutes, the absorbance of OD412nm was measured and the glutathione concentration was calculated by the following equation:
GSH(μM)=[(ODsample-ODBlank)/(ODCalibrator-ODBlank)]×100×n
wherein ODsampleIs the absorbance, OD, of SampleBlankIs the absorbance, OD, of BlankCalibratorThe absorbance of Calibrator and n is the dilution factor.
Hereinafter, referring to FIGS. 1 to 7, the results of animal experiments using the lactic acid bacteria strain having the effect of protecting liver activity according to the present invention will be described. FIG. 1 is a graph showing the results of animal experiments with alanine Aminotransferase (ALT) in the serum of groups of mice fed with the lactic acid bacteria strain of the present invention. As can be seen from the results in fig. 1, the alcohol group was significantly higher (p < 0.01) at week 2 (2W) and week 4 (4W) compared to the control group, and it was shown that alcohol caused an increase in the liver inflammation index ALT as indicated by # in fig. 1. While feeding the lactic acid bacteria strains of the present invention significantly reduced ALT values, wherein the symbols in figure 1 indicate that the group of alcohol plus lactic acid bacteria strains of the present invention at the same time point showed a significant or very significant reduction in the alcohol-induced increase in the liver inflammation index ALT (p, p < 0.05;. p, p < 0.01). At week 2, the ALT value of TSP05 group was decreased from 57 to 36, the ALT value of TSF331+ TSR332 group was decreased from 57 to 33, and the ALT value of TSP05+ TSF331+ TSR332 group was decreased from 57 to 28; at week 4, the ALT value of TSP05 group was decreased from 49 to 35, the ALT value of TSF331+ TSR332 group was decreased from 49 to 31, and the ALT value of TSP05+ TSF331+ TSR332 group was decreased from 49 to 25. The three groups fed with the lactobacillus strains of the invention achieve significant differences, wherein the mixed TSP05, TSF331 and TSR332 strains have better use effects. There was no significant difference between the groups fed with the lactic acid bacteria strain of the present invention in the second and fourth weeks.
Please refer to fig. 2, which shows the results of animal experiments on glutamic-oxaloacetic transaminase (AST) in serum of various groups of mice after feeding the lactobacillus strains of the present invention. As can be seen from the results shown in FIG. 2, the ALT level in the serum was significantly or significantly higher in both the alcohol groups at weeks 2 and 4 (#, p < 0.01; #, p < 0.05), and thus, the liver inflammation index AST was increased by alcohol. Feeding the lactic acid bacteria strain of the present invention can significantly reduce the AST value. At week 2, the AST value of TSP05 group decreased from 73 to 59, the AST rise by alcohol was significantly reduced compared to the alcohol group (, p < 0.05), the AST value of TSF331+ TSR332 group decreased from 73 to 63, the AST value of TSP05+ TSF331+ TSR332 group decreased from 73 to 54, and there was a very significant decrease compared to the alcohol group (, p < 0.01); at week 4, the AST value of TSP05 group decreased from 89 to 76, the AST value of TSF331+ TSR332 group decreased from 89 to 72, and the AST value of TSP05+ TSF331+ TSR332 group decreased from 89 to 66. The three groups fed with the lactic acid bacteria strain of the present invention all had the effect of reducing AST, and among them, the effect of using three strains of TSP05, TSF331 and TSR332 in combination was better. There was no significant difference between the groups fed with the lactic acid bacteria strain of the present invention in the second and fourth weeks.
Please refer to fig. 3, which shows the results of animal experiments on triglycerides in the serum of various groups of mice fed with the lactobacillus strain of the present invention. At week 2, the triglyceride values in each group were significantly higher than those measured at week 0 (0W), with the alcohol group (alcohol group) being significantly higher than the control group, as indicated by the # in fig. 3, and at week 4, the alcohol group was different but not significantly higher than the control group (p ═ 0.08). The TSP05 group, the TSF331+ TSR332 group and the TSP05+ TSF331+ TSR332 group can reduce triglyceride values at 2 weeks and 4 weeks, wherein the TSF331+ TSR332 group has better effect of reducing triglyceride in serum, and the significant difference (p is less than 0.05) is achieved.
Please refer to fig. 4, which shows the results of animal experiments on total cholesterol in serum of various groups of mice fed with the lactobacillus strain of the present invention. At week 2, the total cholesterol values were higher for each group than for week 0, with no significant difference between groups. At week 4, the alcohol-fed groups were not significantly different but were lower than the alcohol-unfed groups (control group and Mix-LAB group), indicating that the lactic acid bacterium strain of the present invention did not change the serum cholesterol concentration within week 4.
Referring to fig. 5, which is a result of an animal test of triglycerides in livers of respective groups of mice fed with the lactic acid bacterium strain of the present invention, wherein symbols, # and # indicate that the respective groups are significantly different from the control group, and symbols # and # indicate that the groups fed with alcohol plus the lactic acid bacterium strain of the present invention are significantly different from the alcohol group. From the results of fig. 5, it is understood that the triglyceride concentration (μ M/g) in the liver of the alcohol group was significantly increased from 39 μ M/g to 76 μ M/g. The feeding of the lactobacillus strain of the invention can obviously reduce the triglyceride concentration in the liver, wherein the triglyceride concentration in the liver of TSP05 group is reduced to 50 mu M/g, the triglyceride concentration in the liver of TSF331+ TSR332 group is reduced to 49 mu M/g, and the triglyceride concentration in the liver of TSP05+ TSF331+ TSR332 group is reduced to 51 mu M/g, which reach a significant difference, thereby avoiding the formation of fatty liver. There were no significant differences between the groups fed with the lactic acid bacteria strains of the present invention.
Referring to fig. 6, which is the results of animal experiments on glutathione in livers of various groups of mice fed with the lactic acid bacterium strain of the present invention, symbols # and # indicate that the groups are significantly different from the control group, and symbol # indicates that the group fed with alcohol plus the lactic acid bacterium strain of the present invention is significantly different from the alcohol group. From the results of fig. 6, it was found that the glutathione concentration in the liver was significantly decreased in the alcohol group from 925 μ M/g to 590 μ M/g, indicating that alcohol causes oxidative stress. The concentration of glutathione in the liver can be obviously improved by feeding the groups of the lactobacillus strains, and the three groups have the effect of improving the concentration of glutathione so as to reduce the oxidation pressure of the liver, wherein the effect of mixing three strains TSP05, TSF331 and TSR332 is better.
Referring to fig. 7, which is the result of animal experiments on glutathione peroxidase activity in livers of respective groups of mice fed with the lactic acid bacterium strain of the present invention, wherein symbols are marked as very significant differences between the respective groups and the control group, and symbols # indicate that the groups fed with alcohol plus the lactic acid bacterium strain of the present invention are very significant differences between the alcohol group. From the results shown in fig. 7, it can be seen that the glutathione peroxidase activity in the liver of the alcohol group was not significantly changed compared to the control group, and the group fed with the lactobacillus strain of the present invention can significantly increase the glutathione peroxidase activity in the liver, thereby increasing the antioxidant ability.
In combination with the above test results, the lactic acid bacteria strain-containing food composition and pharmaceutical composition of the present invention can protect the liver and prevent liver inflammation and fatty liver, and can improve the antioxidant ability of the liver to prevent the liver from being damaged by the oxidative stress caused by alcohol. The invention provides a lactic acid bacteria strain which has no side effect on human body and can protect liver as a new choice for protecting liver.
The above-mentioned embodiments are merely illustrative of the technical spirit and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the content of the present invention and to implement the same, so that the scope of the present invention should not be limited by the above-mentioned embodiments, and that all equivalent changes and modifications made in the spirit of the present invention should be covered by the scope of the present invention.
Claims (13)
1. A food composition comprising a lactic acid bacterial strain comprising:
a lactic acid bacterial strain having an effect of protecting liver activity, the lactic acid bacterial strain being selected from at least one of the following isolated lactic acid bacterial strains: lactobacillus plantarum TSP05 strain with preservation number of CGMCCNo.16710; lactobacillus fermentum (Lactobacillus fermentum) TSF331 strain with preservation number of CGMCCNo.15527; and Lactobacillus reuteri TSR332 with the preservation number of CGMCC No.15528, wherein the Lactobacillus strains are all preserved in the common microorganism center of China Committee for culture Collection of microorganisms; and
a physiologically acceptable excipient, diluent or carrier.
2. The lactic acid bacteria strain-containing food composition according to claim 1, wherein the lactic acid bacteria strain comprises Lactobacillus plantarum TSP05 strain with accession number CGMCC No. 16710.
3. The lactic acid bacteria strain-containing food composition according to claim 1, wherein the lactic acid bacteria strain comprises the Lactobacillus fermentum TSF331 strain having the accession number CGMCC No. 15527; and Lactobacillus reuteri TSR332 with the preservation number of CGMCC No. 15528.
4. The lactic acid bacteria strain-containing food composition according to claim 1, wherein the lactic acid bacteria strain comprises Lactobacillus plantarum TSP05 strain, accession number CGMCC No. 16710; lactobacillus fermentum TSF331 strain with preservation number of CGMCC No. 15527; and Lactobacillus reuteri TSR332 with the preservation number of CGMCC No. 15528.
5. The lactic acid bacterial strain-containing food composition according to claim 1, wherein the lactic acid bacterial strain is an active strain.
6. A lactic acid bacteria strain-containing food composition according to claim 1 wherein the excipient, diluent or carrier is a food product.
7. The lactic acid bacteria strain-containing food composition of claim 6, wherein the food product comprises fermented milk, yogurt, cheese, milk drink powder, tea, coffee, chewing gum, dentrifice, or a combination thereof.
8. A pharmaceutical composition comprising a lactic acid bacterial strain, comprising:
a lactic acid bacterial strain having an effect of protecting liver activity, the lactic acid bacterial strain being selected from at least one of the following isolated lactic acid bacterial strains: lactobacillus plantarum TSP05 strain with preservation number of CGMCCNo.16710; lactobacillus fermentum (Lactobacillus fermentum) TSF331 strain with preservation number of CGMCCNo.15527; and Lactobacillus reuteri TSR332 with the preservation number of CGMCC No.15528, wherein the Lactobacillus strains are all preserved in the common microorganism center of China Committee for culture Collection of microorganisms; and
a pharmaceutically acceptable excipient, diluent or carrier.
9. The lactic acid bacteria strain-containing pharmaceutical composition according to claim 8, wherein the lactic acid bacteria strain comprises Lactobacillus plantarum TSP05 strain with accession number CGMCC No. 16710.
10. The lactic acid bacteria strain-containing pharmaceutical composition according to claim 8, wherein the lactic acid bacteria strain comprises the Lactobacillus fermentum TSF331 strain having the accession number CGMCC No. 15527; and Lactobacillus reuteri TSR332 with the preservation number of CGMCC No. 15528.
11. The lactic acid bacteria strain-containing pharmaceutical composition according to claim 8, wherein the lactic acid bacteria strain comprises Lactobacillus plantarum TSP05 strain, accession number CGMCC No. 16710; lactobacillus fermentum TSF331 strain with preservation number of CGMCC No. 15527; and Lactobacillus reuteri TSR332 with the preservation number of CGMCC No. 15528.
12. The lactic acid bacteria strain-containing pharmaceutical composition according to claim 8, wherein the lactic acid bacteria strain is an active strain.
13. The lactic acid bacterial strain-containing pharmaceutical composition according to claim 8, which is an oral dosage form.
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CN115039883A (en) * | 2021-02-26 | 2022-09-13 | 锦乔生物科技有限公司 | Use of Lactobacillus plantarum TSP05 isolate for reduction of purine content and uric acid levels |
CN115039883B (en) * | 2021-02-26 | 2024-05-10 | 锦乔生物科技有限公司 | Use of lactobacillus plantarum TSP05 isolates to reduce purine content and uric acid levels |
CN113046274A (en) * | 2021-04-26 | 2021-06-29 | 江南大学 | Lactobacillus reuteri for relieving gastrointestinal injury caused by capsaicin and application thereof |
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