CN103704348A - Donkey milk powder formula for boosting brain, nourishing blood and improving resistance based on homology of medicine and food - Google Patents
Donkey milk powder formula for boosting brain, nourishing blood and improving resistance based on homology of medicine and food Download PDFInfo
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- CN103704348A CN103704348A CN201310693299.9A CN201310693299A CN103704348A CN 103704348 A CN103704348 A CN 103704348A CN 201310693299 A CN201310693299 A CN 201310693299A CN 103704348 A CN103704348 A CN 103704348A
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Abstract
The invention discloses a donkey milk powder formula for boosting brain and nourishing blood based on homology of medicine and food. According to the formula, donkey milk powder serves as a principle component; the donkey milk powder formula can obviously improve the immunity of mice with low immunity, and the donkey milk powder formula has the specific effects of obviously improving the lymphocyte transformation capacity (namely the cellular immune function), increasing the production amount of serum hemolysin (namely the humoral immune function) and improving the carbon clearance capacity (namely the mononuclear macrophage function). Glucose and maltodextrin added into the donkey milk powder formula have the effect of improving the human digestive function in an assisted mode. Moreover, the added rhodiola rosea, almonds and qamgur and the like available for the homology of medicine and food have the effects of boosting brain, nourishing blood and improving the human digestive function.
Description
Technical field
The invention belongs to field of health care food, is a kind of health care donkey milk powder specifically.
Background technology
Donkey milk, owing to having good drug effect, at home and abroad has in ancient books and records more and relates to.In Ru China < < Compendium of Materia Medica > >, record: " donkey breast, smell is sweet, cold profit is nontoxic, heat frequently drink can control the obstruction of the circulation of vital energy, separate children's's heat poison, do not give birth to acne rash "; In the minority name family medical science such as Uygur and Mongolia, useful donkey milk treatment tuberculosis, cirrhosis and gastric ulcer, pertussal record.In addition, Peruvian thinks that donkey milk can control the diseases such as asthma, tracheitis, diabetes.Yet, at present the research of donkey milk is mainly concentrated on the analysis of its nutritional labeling, and lack on aspect the comprehensive development and utilization of donkey milk, go deep into systematic research.
Rhodiola root: the applicating history of rhodiola root is long, before more than 2,000 year, the people of Qinghai-Tibet Platean is just used as medicine with it, to improve the health, the impact of opposing poor environment.Among the peoplely be commonly used to decoct water or steep in wine, to banish fatigue or to resist mountain area cold.Simultaneously and diseases prevention is healthy and strong and nourishing lengthens one's life.Because of its magical effect that has strengthening healthy qi, benefiting qi and nourishing blood, enriching yin benefit lung, successive dynasties Tibetan medicine is regarded as " lucky Triratna ".The adaptation former state effect of rhodiola root, can promote the nonspecific resistance of human body to destructive stimulus, strengthens the adaptability of human body.See through the oxygen diffusion of reinforcement inner cell, or oxygen uses benefit, oxidation resistant ability, help and improve the adaptive faculty that health stimulates anoxic.Be used for: improve sleep, blood-enriching and blood-promoting circulation, anti-cerebral anoxia, antifatigue, promoting blood circulation and hemostasis, removing heat from the lung to relieve cough, change become silted up detumescence, antipyreticly bring down a fever, nourish the effects such as vigour; Be used for the treatment of traumatic injury and burn and scald outward.
Ba Danmu: scientific research shows, Ba Danmu is containing one of maximum full nutriment of vitamin E, and vitamin E can, effectively to Green Tea Extract, play the effect of preserving moisture and protecting skin and delaying senility.In Ba Danmu, up to 70% the helpful reduction of unrighted acid " bad " cholesterol levels, edible Ba Danmu, can effectively reduce body's cholesterol and triglyceride content, reduces the potential threat of heart attack.The demonstration of correlative study result, Ba Danmu has prebiotics characteristic, can improve intestinal health by increasing the beneficial bacterium in enteron aisle, promotes defecation.< < Britain nutrition periodical > > once delivered the professor Richard of food and nutrition system of Guo Yixiang Purdue Univ-West Lafayette USA. the research of Metronix doctor research team, result shows: edible bar dawn wood can allow people produce obvious satiety, thereby helps to control the absorption for other high heat food; Secondly, the contained dietary fiber of bar dawn wood reduces by fat absorption rate, thereby effectively controls body weight.
Just agate is ancient: the principal of Xinjiang Medicine University, the special allowance expert of State Council, doctoral advisor breathe out wood and draw and propose my Fu Er research and find, the proper agate Gu that is grown in Xinjiang is the rare a kind of plant of being rich in organic active alkali of occurring in nature.Except being rich in the wholesome Alkaline minerals elements such as calcium, iron, zinc, potassium and organic-biological alkali, also contain the materials such as 19 seed amino acids, protein, crude fibre, linoleic acid, flavonoids, polysaccharide, saponin, a kind of than the more rational plant roots and stems of ginseng, can to each internal organs of health, carry out comprehensive nutrition adjusting simultaneously, reach acid-base balance, improve the effect of physique.
Therefore, carry out the applied basic research of donkey milk powder compound prescription, widen its application approach, there are important economic results in society.
Summary of the invention
The present invention is intended to provide new way for the market development of donkey milk, take donkey milk powder as matrix, is aided with the rhodiola root, Ba Danmu, the Qia Magu that add prebiotics, probio and integration of drinking and medicinal herbs, finds a kind of formula donkey milk powder that strengthens immunity of organisms.
Technical solution of the present invention is as follows:
Based on integration of drinking and medicinal herbs benefit brain, nourish blood and improve a formula donkey milk powder for resistance, in donkey milk powder, add rhodiola root, Ba Danmu, Qia Magu that prebiotics, probio and integration of drinking and medicinal herbs can be used; Mainly composed of the following components by weight: donkey milk powder: 900 parts, prebiotics: 1 part, probio: 0.5 part, integration of drinking and medicinal herbs raw material: 5 parts, DEXTROSE ANHYDROUS: 70 parts, maltodextrin: 23.5 parts.
Described prebiotics is the mixture of FOS, synanthrin and galactooligosaccharide; Ratio is preferably 1:1:1.
It is 5.0 * 10 that described probio adopts bacterium powder viable count
10the Lactobacillus rhamnosus of cfu/g.
Described integration of drinking and medicinal herbs material is rhodiola root, Ba Danmu, Qia Magu, and weight ratio is 1:2:2.
For evaluating the present invention, on the basis of donkey milk powder, further strengthen immunity of organisms, adopt intraperitoneal injection of cyclophosphamide to prepare after immunocompromised mouse, by the continuous gavage of gavage group difference 28 days.
After gavage finishes, get mouse spleen, grind and obtain SPL, add ConA and stimulate lymphocyte, observe the lymphocytic conversion capability of different gavage group mouse.
After gavage finishes, by tail vein injection prepared Chinese ink, evaluate the carbon of different gavage group mouse and clean up ability.
Before gavage finishes the 4th day, lumbar injection Mianyang erythrocyte is got blood after gavage finishes, and measures the generation of serum hemolysin.
The specific embodiment:
Embodiment 1
Based on integration of drinking and medicinal herbs benefit brain, nourish blood and improve a formula donkey milk powder for resistance, in donkey milk powder, add rhodiola root, Ba Danmu, Qia Magu that prebiotics, probio and integration of drinking and medicinal herbs can be used; Mainly composed of the following components by weight: donkey milk powder: 900 parts, prebiotics: 1 part, probio: 0.5 part, integration of drinking and medicinal herbs raw material: 5 parts, DEXTROSE ANHYDROUS: 70 parts, maltodextrin: 23.5 parts;
Described prebiotics is the mixture of FOS, synanthrin and galactooligosaccharide; Ratio is preferably 1:1:1;
It is 5.0 * 10 that described probio adopts bacterium powder viable count
10the Lactobacillus rhamnosus of cfu/g;
Described integration of drinking and medicinal herbs material is rhodiola root, Ba Danmu, Qia Magu, and weight ratio is 1:2:2.
Embodiment 2
The preparation of immunocompromised mouse: buy 72 of BALB/c mouses, place Animal House and observe after one week, the next day intraperitoneal injection of cyclophosphamide solution, first day is by 100 mg/kg dosage injections, the 3rd day by 80 mg/kg dosage injections, preparation immunocompromised mouse.
The setting of mouse stomach group: mouse is divided into 3 groups at random, 10 every group, is followed successively by blank group, donkey milk group, health care donkey milk powder group.Every group of mouse stomach phase is 28 days, and the gavage amount of every mouse was 300 μ L/ days.The SPSS of the mouse stomach 0.85% of blank group, the mouse of donkey milk powder group and formula donkey milk powder group is by the dosage gavage of 5 g/kg.
The preparation of splenocyte suspension: adopt strength vertebra dislocation method to put to death mouse, in alcohol immersion 2 min with 75%, disinfect.Mouse is placed in and dissects pallet, along peritonaeum, dissect mouse, the aseptic spleen of getting, is placed in and fills appropriate aseptic Hank's liquid plate, removes the connective tissue of adhesion on spleen, and after washing, spleen is transferred in aseptic Hank's liquid plate gently.With tweezers, spleen is ground gently on 40 order gauzes, after 200 eye mesh screens filter, obtain single cell suspension.Cell suspension, after centrifugal 10 min of 1000 rpm, adds erythrocyte cracked liquid in 10:1 ratio, and piping and druming mixes gently, pyrolysis time approximately 5 min left and right.Centrifugal 10 min of 1000 rpm, remove supernatant, aseptic Hank's liquid washing 2 times for cell precipitation.Cell is suspended in 1 mL RPMI1640 complete culture solution, with platform, expects blue dyeing counting viable count (should more than 95%), adjusting cell concentration is 3 * 10
6individual/mL.
The mensuration of cellular immune function---mouse lymphocyte transformation experiment: divide 4 holes to add in 24 porocyte culture plates every a splenocyte suspension, every hole 1 mL, wherein 3 holes are as Duplicate Samples hole, all add 50 μ L ConA liquid (being equivalent to 5 μ g/mL), in residue 1 hole, add 50 μ L cell culture fluids to replace ConA liquid as blank well.Put 5% CO
2, in 37 ℃ of incubators, cultivate 72 h; Cultivation finishes front 4 h, and every hole sucks 0.7 mL supernatant gently, then adds 0.7 mL not containing the RPMI1640 nutrient solution of serum, and every hole adds 50 μ LMTT solution (5 mg/mL) simultaneously, continues to cultivate 4 h.After cultivation finishes, every hole adds l mL acid isopropyl alcohol, and fully piping and druming mixes, and purple crystal is dissolved completely.Then divide and install in 96 well culture plates, 3 parallel holes are made in each hole, measure extinction OD value the record in each hole under ELIASA 570 nm wavelength.
The impact (X ± SD) that the different gavage group of table 1 transforms the SPL of Con-A induction
| Group | Dosage | Size of animal/only | ConA induction spleen lymphocyte proliferation OD difference |
| Blank group | 0.85% physiological saline | 10 | 0.229±0.102 |
| Donkey milk powder group | 5 g/kg | 10 | 0.251±0.087 |
| Formula donkey milk powder group | 5 g/kg | 10 | 0.314±0.053 a b |
Note: a represents to compare with blank group, p<0.05, and b represents to compare with donkey milk powder group, p<0.05.
Experimental result is as shown in table 1, result shows: compare with physiological saline group, the donkey milk powder of gavage one-component can not improve the conversion ratio (p>0.05) of SPL, and the SPL conversion ratio of gavage formula donkey milk powder group mouse is significantly higher than the mouse (p<0.05) of donkey milk powder group.
The mensuration of humoral immune function---serum hemolysin experiment: in the time of the 28th day, mouse is carried out the SRBC of lumbar injection 0.2 mL 2% in gavage, immunity taked eye socket to get blood after the 5th day.By the blood sample of collecting, in 37 ℃ of standing 1 h, 2,000 4 ℃ of rpm refrigerated centrifuge, 10 min, carefully draw serum, avoid sucking erythrocyte.Serum supplies to measure with 200 times of SA buffer solution dilutions.The mice serum that adds successively l mL dilution in example reaction pipe, 0.5 mL SRBC(10%), the complement of l mL dilution; Blank Guan Zhongyong 1 mL SA buffer solution replaces blood serum sample, after fully mixing, in 37 ℃ of waters bath with thermostatic control, is incubated 30 min, moves to cessation reaction in ice bath.Centrifugal 10 min of 2000 rpm, get l mL supernatant and add 3 mL Dou Shi reagent, shake up and place 10 min.With blank pipe, make blank, 540 nm wavelength colorimetrics read absorbance.The mensuration of SRBC HD50 value: get 0.25 mL SRBC(5%) add Dou Shi liquid to 4 mL, 540 nm wavelength colorimetrics read absorbance, the absorbance while being SRBC HD50 used in experiment.Example reaction pipe HD50 value CH
50be calculated as follows: the change of serum C H of every mouse
50absorbance * extension rate during absorbance/SRBC HD50 of=sample.
The different gavage group of table 2 is on the impact of mice serum hemolysin (X ± SD)
| Group | Dosage | Size of animal/only | Serum hemolysin |
| Blank group | 0.85% physiological saline | 10 | 159.62±6.14 |
| Single donkey milk powder group | 5 g/kg | 10 | 168.72±4.97 a |
| Formula donkey milk powder group | 5 g/kg | 10 | 177.31±4.27 a b |
Note: a represents to compare with blank group, p<0.05, and b represents to compare with donkey milk powder group, p<0.05.
Experimental result is as shown in table 2, result shows: aspect the generation of mice serum hemolysin, compare with the blank group of physiological saline, single donkey milk powder group all has significant difference (p<0.05) with formula donkey milk powder group, but formula donkey milk powder group increases the generation of serum hemolysin significantly than single donkey milk powder group.
The mensuration of monocytes/macrophages phagocytic activity---mouse carbon is cleaned up experiment: injection prepared Chinese ink: by 0.1 mL/kg dosage from mouse tail vein injection prepared Chinese ink, after Ink injection, timing immediately.2 min and 8 min after injection prepared Chinese ink, get blood 20 μ L from angular vein clump respectively, and existing side by side, soon it is added to 2 mL 0.1% Na
2cO
3in solution, fully mix.In 600 nm wavelength place's photometry density value OD values, with Na
2cO
3solution is made blank.Mouse is put to death, get liver and spleen, with filter paper, blot organ surface blood stains, the record of weighing respectively.With phagocytic index a, represent the ability that mouse carbon is cleaned up, be calculated as follows phagocytic index a:
The different gavage group of table 3 is cleaned up the impact (X ± SD) of ability on mouse carbon
| Group | Dosage | Size of animal/only | Phagocytic index/k | Proofread and correct phagocytic index/a |
| Blank group | 0.85% physiological saline | 10 | 0.018±0.004 | 5.038±0.514 |
| Single donkey milk powder group | 5 g/kg | 10 | 0.023±0.011 | 5.562±0.419 |
| Formula donkey milk powder group | 5 g/kg | 10 | 0.038±0.011 | 6.964±0.394 a b |
Note: a represents to compare with blank group, p<0.05, and b represents to compare with donkey milk powder group, p<0.05.
Experimental result is as shown in table 3, and result shows: formula donkey milk powder group can significantly strengthen mouse carbon and clean up ability (p<0.05).
Claims (4)
1. based on integration of drinking and medicinal herbs benefit brain, nourish blood and improve a formula donkey milk powder for resistance, it is characterized in that: in donkey milk powder, add rhodiola root, Ba Danmu, Qia Magu that prebiotics, probio and integration of drinking and medicinal herbs can be used; Composed of the following components by weight: donkey milk powder: 900 parts, prebiotics: 1 part, probio: 0.5 part, integration of drinking and medicinal herbs raw material: 5 parts, DEXTROSE ANHYDROUS: 70 parts, maltodextrin: 23.5 parts.
2. the donkey milk powder of filling a prescription as described in claim 1, is characterized in that: described prebiotics is the mixture of FOS, synanthrin and galactooligosaccharide.
3. the donkey milk powder of filling a prescription as described in claim 1, is characterized in that: it is 5.0 * 10 that described probio adopts bacterium powder viable count
10the Lactobacillus rhamnosus of cfu/g.
4. the donkey milk powder of filling a prescription as described in claim 1, is characterized in that: described integration of drinking and medicinal herbs material is rhodiola root, Ba Danmu, Qia Magu, weight ratio is 1:2:2.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104187472A (en) * | 2014-08-26 | 2014-12-10 | 邢万善 | Donkey milk and seed melon nutrition powder |
| CN104686657A (en) * | 2015-02-11 | 2015-06-10 | 新疆世全黄金矿业股份有限公司 | Formula donkey milk powder with anti-tumor efficacy |
| CN105410190A (en) * | 2015-12-23 | 2016-03-23 | 南达新农业股份有限公司 | Production process of nutritional and health-caring Qamgur milk |
| CN106306028A (en) * | 2016-08-28 | 2017-01-11 | 新疆玉昆仑天然食品工程有限公司 | Donkey milk brassica campestris powder and preparation method thereof |
| CN106880035A (en) * | 2015-12-15 | 2017-06-23 | 范旻 | A kind of total enteral nutritious product of regulating intestinal canal flora imbalance and preparation method thereof |
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Patent Citations (5)
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| CN1775092A (en) * | 2005-11-22 | 2006-05-24 | 余内逊 | Method for preparing micron milk powder with sugared ginsing, testudinate, chromium and propolis for reducing sugar and removing fatigue |
| CN101695316A (en) * | 2009-11-11 | 2010-04-21 | 天津商业大学 | Milk powder with function of strengthening immunity and production method thereof |
| CN101810222A (en) * | 2010-04-23 | 2010-08-25 | 陕西农产品加工技术研究院 | Synbiotic goat milk powder |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104187472A (en) * | 2014-08-26 | 2014-12-10 | 邢万善 | Donkey milk and seed melon nutrition powder |
| CN104686657A (en) * | 2015-02-11 | 2015-06-10 | 新疆世全黄金矿业股份有限公司 | Formula donkey milk powder with anti-tumor efficacy |
| CN106880035A (en) * | 2015-12-15 | 2017-06-23 | 范旻 | A kind of total enteral nutritious product of regulating intestinal canal flora imbalance and preparation method thereof |
| CN105410190A (en) * | 2015-12-23 | 2016-03-23 | 南达新农业股份有限公司 | Production process of nutritional and health-caring Qamgur milk |
| CN106306028A (en) * | 2016-08-28 | 2017-01-11 | 新疆玉昆仑天然食品工程有限公司 | Donkey milk brassica campestris powder and preparation method thereof |
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Application publication date: 20140409 |