CN104634984A - Fully-automatic fluorescence immunoassay system - Google Patents
Fully-automatic fluorescence immunoassay system Download PDFInfo
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- CN104634984A CN104634984A CN201510068101.7A CN201510068101A CN104634984A CN 104634984 A CN104634984 A CN 104634984A CN 201510068101 A CN201510068101 A CN 201510068101A CN 104634984 A CN104634984 A CN 104634984A
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- 238000003018 immunoassay Methods 0.000 title abstract description 8
- 238000001514 detection method Methods 0.000 claims abstract description 27
- 238000012360 testing method Methods 0.000 claims abstract description 26
- 238000012545 processing Methods 0.000 claims abstract description 13
- 238000013016 damping Methods 0.000 claims description 47
- 239000012530 fluid Substances 0.000 claims description 47
- 230000007613 environmental effect Effects 0.000 claims description 7
- 230000003993 interaction Effects 0.000 claims description 7
- 239000011259 mixed solution Substances 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 5
- 238000000926 separation method Methods 0.000 claims description 4
- 239000007853 buffer solution Substances 0.000 abstract 4
- 238000012864 cross contamination Methods 0.000 abstract 1
- 108010074051 C-Reactive Protein Proteins 0.000 description 6
- 102100032752 C-reactive protein Human genes 0.000 description 6
- 210000004369 blood Anatomy 0.000 description 5
- 239000008280 blood Substances 0.000 description 5
- 230000004054 inflammatory process Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000012544 monitoring process Methods 0.000 description 3
- 206010061218 Inflammation Diseases 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000000105 evaporative light scattering detection Methods 0.000 description 2
- 210000004700 fetal blood Anatomy 0.000 description 2
- 210000000265 leukocyte Anatomy 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 206010020633 Hyperglobulinaemia Diseases 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000000721 bacterilogical effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004820 blood count Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 238000010185 immunofluorescence analysis Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000003475 lamination Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000002980 postoperative effect Effects 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention discloses a fully-automatic fluorescence immunoassay system comprising a suction head placing frame, a sample uniformly-shaking unit, a buffer solution feeding unit, a test paper sheet conveying unit, a three-dimensional sample adding unit, a detection unit and a signal processing control unit, wherein a suction head is placed on the suction head placing frame; the sample uniformly-shaking unit is used for uniformly shaking a to-be-detected sample; the buffer solution feeding unit is used for conveying a buffer solution plate to a working position, and the buffer solution plate is abandoned after running out; the test paper sheet conveying unit is used for pushing out test paper sheets from a test paper box and respectively conveying the test paper sheets to a sample adding position and a detection position; the three-dimensional sample adding unit is used for loading the suction head; and the detection unit is used for detecting the test paper sheets which are subjected to sample adding, and the test paper sheets are abandoned after detection ends. According to the fully-automatic fluorescence immunoassay system disclosed by the invention, the strength and error rate of detection operations can be significantly reduced by virtue of fully-automatic operations, the detection throughput can reach 60 tests per hour, and thus the requirements of large batch detection can be met; and according to the fully-automatic fluorescence immunoassay system, the suction and mixing of the samples and the sample adding of the test paper sheets are performed by using the disposable suction heads, so that the cross contamination among the samples can be avoided, and the accuracy of results can be improved.
Description
Technical field
The present invention relates to fluoroimmunoassay field, particularly the full-automatic fluoroimmunoassay system of one.
Background technology
C reactive protein (CRP) is the inflammation index that the very high Idiotype of clinical conventional susceptibility is very low, at present as the conventional sense project of hospital, as auxiliary judgment foundation in a lot of medical diagnosis on disease.The mensuration of CRP can not be subject to the impact of the factors such as age, sex, anaemia, hyperglobulinemia, gestation, can be used for the discriminating that auxiliary white blood cell count(WBC) and esr detection carry out between bacterium and non-bacterial, judge that the degree of tissue inflammation or damage is conducive to observing the reaction of patient for treatment and postoperative detection.And Fast Measurement CRP, in order to instruct clinical application fast, the patient's abuse of antibiotics to virus infections or possibility self limiting epidermis bacteriological infection can be avoided.
The detection of CRP is mainly based on the turbid and immune scattering ratio purifying method of immune transmittance, but the detection lowest limit is generally all greater than 3 ~ 5mg/L, and lower sensitivity is not enough to the judgement to condition assessment and curative effect of medication.In recent years, the employing in succession of fluorescence immune analysis method substantially increases the sensitivity of analysis, and the detection lowest limit is 0.005 ~ 0.1mg/L, result is stablized, and degree of accuracy is high, and sample consumption is few, can adopt tip trace blood, the misery brought to patient is few, thus obtains and develops fast.
But mostly at present be semi-automatic immunofluorescence analysis instrument for what detect CRP, only comprise detecting unit and control system thereof, be added drop-wise to test-paper from the mixing of the absorption of blood sample, sample and damping fluid, mixed liquor, to send into detecting position be all manual operation to test-paper, workload is large, efficiency is low, especially when there being blood sample in enormous quantities to need to detect, there will be the inaccurate of the result caused due to human operational error unavoidably, affect the judgement of doctor to the examination of patient's inflammatory process, monitoring, assessment and curative effect of medication.
Summary of the invention
An object of the present invention is to solve at least the problems referred to above and/or defect, and the advantage will illustrated at least is below provided.
A further object of the invention is to provide a kind of full-automatic fluoroimmunoassay system, it integrates whole blood sample and shakes up unit, damping fluid feed unit, test-paper supply unit, three-dimensional application of sample unit and detecting unit, full-automatic operation significantly reduces intensity and the error rate of testing, can meet the demand of mass detection.
In order to realize according to these objects of the present invention and other advantages, provide a kind of full-automatic fluoroimmunoassay system, comprising:
Suction nozzle rack, it is placed with suction nozzle;
Sample shakes up unit, and measuring samples shakes up by it, and when preventing from sampling, sample generation lamination, affects testing result;
Damping fluid feed unit, the damping fluid plate being mounted with damping fluid is delivered to working position by it, and described damping fluid plate is abandoned after depletion;
Test-paper supply unit, paper box rotates to working position by it, is released by test-paper from described paper box, and respectively described test-paper is delivered to respectively load position and detect position;
Three-dimensional application of sample unit, its load described suction nozzle draw successively damping fluid described in described measuring samples, compressing obtain described sample and described damping fluid mixed solution, draw after described mixed solution drops to described test-paper, abandon described suction nozzle;
Detecting unit, it detects described test-paper, detects after terminating, abandons described test-paper;
Signal processing control unit, it carries out the process of detection signal, described sample shakes up unit, the control of the motion of described damping fluid feed unit, described test-paper supply unit and described three-dimensional application of sample unit.
Preferably, wherein, also comprise:
Slideway, its one end is connected to described test paper conveyer, and the other end is connected to described detecting unit, and described test-paper is delivered to load position and detection position along described slideway by described test-paper supply unit;
Ash can, it is collected and uses the described suction nozzle terminated, described test-paper and described damping fluid plate, and described ash can is positioned at the exit of described damping fluid feed unit and described detecting unit;
Human-computer interaction device, it comprises display device and operation-interface, and described display device is LED touch screen;
Frame, it comprises top board, base plate, side supports and shell, described sample shakes up unit, described damping fluid feed unit, described test-paper supply unit, described three-dimensional application of sample unit, described detecting unit, described suction nozzle rack and described ash can and is fixed on described base plate, and described signal processing apparatus, described human-computer interaction device and described shell are fixed on described side supports.
Preferably, wherein, described sample shakes up unit top, described damping fluid plate, the described test-paper being placed in load position and described suction nozzle and is arranged on same plane by clamping part, and well arranged, the lateral separation of described plane and fore-and-aft distance should reduce as far as possible, to reduce the range of movement of suction nozzle, the lateral separation of described plane is 30cm ~ 35cm, and the fore-and-aft distance of described plane is 50cm ~ 60cm.
Preferably, wherein, the interface of described slideway and described paper box, lower than the planimetric position of test-paper exit position in described paper box, exports so that described test-paper is released; Described test-paper supply unit push jack position exports lower than described paper box and waits to release test-paper position, ensures each only release test-paper, reduces matching requirements.
Preferably, wherein, described signal processing control unit is also connected with environmental detection unit, described environmental detection unit comprises temperature and moisture sensors, in order to monitor the difference of testing environment and standard environment, described signal processing control device receives described difference value, revises testing result.
Preferably, wherein whether whether, described suction nozzle rack is provided with first sensor, detects described suction nozzle and exist, described ash can periphery is provided with the second sensor, detect described ash can and exist.
Preferably, wherein, on the downside of described damping fluid feed unit working position, be provided with refrigerating plant, Cord blood can be carried out to temporarily carrying out detecting the damping fluid used, make damping fluid can maintain validity in a long time.
Preferably, wherein, described sample is shaken up unit and is shaken up by described sample by rotary motion, and the rotating speed of described rotary motion is 80 ~ 200rpm.
The present invention at least comprises following beneficial effect:
(1) the present invention integrates whole blood sample and shakes up unit, damping fluid feed unit, test-paper supply unit, three-dimensional application of sample unit and detecting unit, full-automatic operation significantly reduces intensity and the error rate of testing, detect flux be 60 tests/hour, the demand of mass detection can be met;
(2) the present invention adopts disposable tip, after a sample detection process terminates, disposable tip is abandoned, three-dimensional application of sample unit is again from the suction nozzle that suction nozzle rack loading one is new, enter the detection circulation of next sample, thus avoid the cross pollution of sample room, improve the accuracy of result;
(3) the present invention is also provided with environmental detection unit, and the difference between the temperature and humidity of monitoring testing environment and standard environment, revises testing result, ensures the Stability and veracity of result.
Accompanying drawing explanation
Fig. 1 is the vertical view of full-automatic fluorescence immunoassay instrument of the present invention;
Fig. 2 is the side view of full-automatic fluorescence immunoassay instrument of the present invention;
Fig. 3 is the left view of full-automatic fluorescence immunoassay instrument of the present invention;
Fig. 4 is the structural representation of suction nozzle rack of the present invention;
Fig. 5 is the course of work of full-automatic fluorescence immunoassay instrument of the present invention.
In figure: 1, base plate, 2, sample shakes up unit, 3, damping fluid feed unit, 4, test-paper supply unit, 5, detecting unit, 6, frame, 7, human-computer interaction device, 8, three-dimensional application of sample unit, 9, ash can, 10, suction nozzle rack, 11, suction nozzle, 12, rotating disk adapter, 13, damping fluid plate, 14, test-paper, 15, paper box, 16, three-dimensional machinery arm, 17, signal control device, 18, slideway, 19, top board, 20, side supports, 21, shell, 22, first sensor, the 23, second sensor.
Embodiment
Below in conjunction with accompanying drawing, the present invention is described in further detail, can implement according to this with reference to instructions word to make those skilled in the art.
Fig. 1 shows according to a kind of implementation of the present invention, comprising:
Suction nozzle rack 10, it is placed with suction nozzle 11; Sample shakes up unit 2, and measuring samples shakes up by it, and testing sample pipe is put into rotating disk adapter 12, and described sample shakes up unit 2 and can control described rotating disk adapter 12 and move and to be shaken up by described sample; Damping fluid feed unit 3, the damping fluid plate 13 being mounted with damping fluid is delivered to working position by it, and described damping fluid plate 13 is abandoned after depletion; Test-paper supply unit 4, it loads the paper box 15 that three can be held test-paper 14 altogether, and paper box 15 is delivered to working position by rotating by it, and is released from paper box 15 by test-paper 14, delivers to load position respectively and detects position; Three-dimensional application of sample unit 8, after its control three-dimensional machinery arm 16 loads described suction nozzle 11, draw successively damping fluid described in described measuring samples, compressing obtain described sample and described damping fluid mixed solution, draw after described mixed solution drops to described test-paper 14, abandon described suction nozzle 11; Detecting unit 5, it detects the test-paper 14 after described application of sample, detects after terminating, abandons described test-paper 14; Signal processing control unit 17, it carries out the process of detection signal, described sample shakes up unit 2, the control of the motion of described damping fluid feed unit 3, described test-paper supply unit 4 and described three-dimensional application of sample unit 8.
Be further used as preferred embodiment, wherein, also comprise: slideway 18, its one end is connected to described test paper conveyer 4, the other end is connected to described detecting unit 5, and described test-paper 14 is delivered to load position and detection position along described slideway 18 by described test-paper supply unit 4; Ash can 9, it collects the described suction nozzle 11, described test-paper 14 and the described damping fluid plate 13 that use and terminate, and described ash can 9 is positioned at the exit of described damping fluid feed unit 3 and described detecting unit 5; Human-computer interaction device 7, it comprises display device and operation-interface, and described display device is LED touch screen; Frame 6, it comprises top board 19, base plate 1, side supports 20 and shell 21, described sample shakes up unit 2, described damping fluid feed unit 3, described test-paper supply unit 4, described three-dimensional application of sample unit 8, described detecting unit 5, described suction nozzle rack 10 and described ash can 9 and is fixed on described base plate 1, and described signal processing apparatus 17, described human-computer interaction device 7 and described shell 21 are fixed on described side supports 20.
Be further used as preferred embodiment, described sample shakes up unit 2 top, described damping fluid plate 13, the described test-paper 14 being placed in load position and described suction nozzle 11 by clamping part at same plane, the lateral separation of described plane is 32cm, and the fore-and-aft distance of described plane is 50cm.
Be further used as preferred embodiment, the interface of described slideway 18 and described paper box 15, lower than the planimetric position of test-paper 14 exit position in described paper box 15, exports so that described test-paper 14 is released.Described test-paper supply unit 4 push jack position exports lower than described paper box 15 and waits to release test-paper 14 position, to ensure each only release test-paper 14, reduces matching requirements.
Be further used as preferred embodiment, described signal processing control unit 17 is also connected with environmental detection unit, described environmental detection unit comprises temperature and moisture sensors, the difference of monitoring testing environment and standard environment, described signal processing control device receives described difference value, revises testing result.
Whether whether be further used as preferred embodiment, described suction nozzle rack 10 is provided with first sensor 22, detect described suction nozzle 11 and exist, described ash can 9 periphery is provided with the second sensor 23, detect described ash can 9 and exist.
Be further used as preferred embodiment, on the downside of described damping fluid feed unit 3 working position, be provided with refrigerating plant, Cord blood can be carried out to temporarily carrying out detecting the damping fluid used, make damping fluid can maintain validity in a long time.
Be further used as preferred embodiment, described sample is shaken up unit 2 and is shaken up by described sample by rotary motion, and the rotating speed of described rotary motion can featured configuration per sample be 80 ~ 200rpm.
The course of work of the present invention is:
Start, described full-automatic fluoroimmunoassay system is carried out self-inspection and carries out initialization operation, is placed on by sample hose in rotating disk adapter 12, starts sample test pattern.Three-dimensional machinery arm 16 moves to above suction nozzle rack 10, after automatic assembling suction nozzle 11, move to pipette samples liquid above sample hose, then move to above damping fluid plate 13, sample liquid and damping fluid are mixed by continuous compressing by the overlay film on pierces buffer liquid plate 13; Meanwhile, paper box 15 rotates to working position by test paper conveyer 4, and test-paper 14 is released paper box 15, until stop motion behind application of sample position.Three-dimensional machinery arm 16 is by after absorption mixing solution to test-paper 14 position application of sample, and simultaneously described test-paper 14 is sent to after detecting unit 5 detects, and test-paper 14 is by throwing to ash can 9, and three-dimensional machinery arm 16 discards described suction nozzle 11, enters next circulation.
Although embodiment of the present invention are open as above, but it is not limited in listed in instructions and embodiment utilization, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other amendment, therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details and illustrates here and the legend described.
Claims (10)
1. a full-automatic fluoroimmunoassay system, is characterized in that, comprising:
Suction nozzle rack, it is placed with suction nozzle;
Sample shakes up unit, and measuring samples shakes up by it;
Damping fluid feed unit, the damping fluid plate being mounted with damping fluid is delivered to working position by it, and described damping fluid plate is abandoned after depletion;
Test-paper supply unit, paper box rotates to working position by it, is released by test-paper from described paper box, and described test-paper is delivered to respectively load position and detect position;
Three-dimensional application of sample unit, its load described suction nozzle draw successively damping fluid described in described measuring samples, compressing obtain described sample and described damping fluid mixed solution, draw after described mixed solution drops to described test-paper, abandon described suction nozzle;
Detecting unit, it detects described test-paper, detects after terminating, abandons described test-paper;
Signal processing control unit, it carries out the process of detection signal, described sample shakes up unit, the control of the motion of described damping fluid feed unit, described test-paper supply unit and described three-dimensional application of sample unit.
2. full-automatic fluoroimmunoassay system as claimed in claim 1, is characterized in that, also comprise:
Slideway, its one end is connected to described test-paper supply unit, and the other end is connected to described detecting unit, and described test-paper is delivered to load position and detection position along described slideway by described test-paper supply unit;
Ash can, it is collected and uses the described suction nozzle terminated, described test-paper and described damping fluid plate;
Human-computer interaction device, it comprises display device and operation-interface;
Frame, it comprises top board, base plate, side supports and shell, described sample shakes up unit, described damping fluid feed unit, described test-paper supply unit, described three-dimensional application of sample unit, described detecting unit, described suction nozzle rack and described ash can and is fixed on described base plate, and described signal processing apparatus, described human-computer interaction device and described shell are fixed on described side supports.
3. full-automatic fluoroimmunoassay system as claimed in claim 1, it is characterized in that, described sample shakes up unit top, described damping fluid plate, the described test-paper being placed in load position and described suction nozzle and is arranged on same plane by clamping part, the lateral separation of described plane is 30cm ~ 35cm, and the fore-and-aft distance of described plane is 50cm ~ 60cm.
4. full-automatic fluoroimmunoassay system as claimed in claim 2, it is characterized in that, the interface of described slideway and described paper box is lower than the planimetric position of test-paper exit position in described paper box, and described test-paper supply unit push jack position exports lower than described paper box and waits to release test-paper position.
5. full-automatic fluoroimmunoassay system as claimed in claim 1, it is characterized in that, described signal processing control unit is also connected with environmental detection unit, described environmental detection unit comprises temperature and moisture sensors, in order to monitor the difference of testing environment and standard environment, described signal processing control unit receives described difference value, revises testing result.
6. full-automatic fluoroimmunoassay system as claimed in claim 2, it is characterized in that, described ash can is positioned at the exit of described damping fluid feed unit and described detecting unit.
7. full-automatic fluoroimmunoassay system as claimed in claim 2, it is characterized in that, described suction nozzle rack is provided with first sensor, detect described suction nozzle and whether exist, whether described ash can periphery is provided with the second sensor, detect described ash can and exist.
8. full-automatic fluoroimmunoassay system as claimed in claim 2, is characterized in that, described display device is LED touch screen.
9. full-automatic fluoroimmunoassay system as claimed in claim 1, is characterized in that, be provided with refrigerating plant on the downside of the working position of described damping fluid feed unit.
10. full-automatic fluoroimmunoassay system as claimed in claim 1, it is characterized in that, described sample is shaken up unit and is shaken up by described sample by rotary motion, and the rotating speed of described rotary motion is 80 ~ 200rpm.
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| CN201510068101.7A CN104634984B (en) | 2015-02-10 | 2015-02-10 | A kind of full-automatic fluorescence immunoassay system |
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| CN105784986A (en) * | 2016-04-29 | 2016-07-20 | 江苏金标世纪生物科技有限公司 | Clamping strip type full-automatic sample loading detection immunity analyzer |
| CN106482974A (en) * | 2016-05-18 | 2017-03-08 | 南京医科大学第附属医院 | Biopsy forcepss specimen Autosampler and its sampling method |
| CN106596984A (en) * | 2016-10-18 | 2017-04-26 | 秦明 | Full-auto fluorescence immune pre-operation four item assay system and detection method thereof |
| CN108020645A (en) * | 2018-02-01 | 2018-05-11 | 长春万成生物电子工程有限公司 | A kind of water analysis outfit |
| CN108508222A (en) * | 2018-06-19 | 2018-09-07 | 苏州鼎实医疗科技有限公司 | A kind of buffer solution plate and buffer solution suction method for full-automatic fluorescence detector |
| CN109212240A (en) * | 2018-09-03 | 2019-01-15 | 中国科学院苏州生物医学工程技术研究所 | High-throughput full-automatic immune detection instrument |
| CN109828106A (en) * | 2019-02-20 | 2019-05-31 | 苏州鼎实医疗科技有限公司 | A kind of full-automatic immunofluorescence analysis device |
| CN110596408A (en) * | 2019-09-20 | 2019-12-20 | 苏州鼎实医疗科技有限公司 | Full-automatic dry immunoassay equipment |
| CN111458530A (en) * | 2020-05-12 | 2020-07-28 | 四川新健康成生物股份有限公司 | Dry-type immunoassay appearance application of sample device |
| CN114236129A (en) * | 2021-12-20 | 2022-03-25 | 江苏集萃纳米应用技术研究所有限公司 | In-vitro immunodiagnosis reagent background luminescence processing system |
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| CN109828106A (en) * | 2019-02-20 | 2019-05-31 | 苏州鼎实医疗科技有限公司 | A kind of full-automatic immunofluorescence analysis device |
| CN109828106B (en) * | 2019-02-20 | 2024-01-30 | 苏州鼎实医疗科技有限公司 | Full-automatic immunofluorescence analytical apparatus |
| CN110596408A (en) * | 2019-09-20 | 2019-12-20 | 苏州鼎实医疗科技有限公司 | Full-automatic dry immunoassay equipment |
| CN110596408B (en) * | 2019-09-20 | 2024-05-17 | 苏州鼎实医疗科技有限公司 | Full-automatic dry-type immunoassay equipment |
| CN111458530A (en) * | 2020-05-12 | 2020-07-28 | 四川新健康成生物股份有限公司 | Dry-type immunoassay appearance application of sample device |
| CN114236129A (en) * | 2021-12-20 | 2022-03-25 | 江苏集萃纳米应用技术研究所有限公司 | In-vitro immunodiagnosis reagent background luminescence processing system |
| CN114236129B (en) * | 2021-12-20 | 2022-09-23 | 江苏集萃中科纳米科技有限公司 | In-vitro immunodiagnosis reagent background luminescence processing system |
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