CN104620827B - Cutting propagation method for ficus tikoua - Google Patents
Cutting propagation method for ficus tikoua Download PDFInfo
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- CN104620827B CN104620827B CN201510072852.6A CN201510072852A CN104620827B CN 104620827 B CN104620827 B CN 104620827B CN 201510072852 A CN201510072852 A CN 201510072852A CN 104620827 B CN104620827 B CN 104620827B
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- cuttage
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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Abstract
The invention discloses a cutting propagation method for ficus tikoua plants. The method includes matching techniques from cutting and medium selection to cutting time, culture conditions and matters needing attention for ficus tikoua plant cutting generation. Traditional cutting techniques are combined with tissue culture lighting techniques, lighting time is reasonably prolonged, and ficus tikoua seedlings can be obtained in a short time. In order to solve the problem that ficus tikoua seeds are not easily obtained and low in yield, the cutting propagation method is high in cutting survival rate and rapid in seedling establishment, seedlings grow robustly and well after surviving, and ficus tikoua can be more effectively protected, developed and used.
Description
Technical field
The invention belongs to the cultivation field of economic forest, it is related to medicinal, ornamental plant cuttage breeding method, specifically
A kind of method of cutting propagation ground loquat.
Background technology
Ground loquat (ficus tikoua) belongs to Moraceae Ficus, also known as ground fruit, pachyrhizus, Fructus Fici tikouae, evergreen perennial wood of crawling
Matter liana, raw elongated adventitious root on stem, section expands, and complete stool has milk, main product in Guangxi, Guizhou, Hunan and other places, in Guizhou and Hunan
West is as Miao ethnic group's conventional crude drugs.Both at home and abroad the pharmaceutical research of this platymiscium is shown, this platymiscium has hypoglycemic, lax flat
The effects such as sliding flesh, antitumor, antibacterial.In addition to fruit can be used for developing beverage, cauline leaf can also be made as the green of livestock
Feed, ground loquat is contacted to earth the elongated adventitious root of life, is easily grown to netted covering, seemingly the carpet of green, can by surface pine scholar,
Sandy soil firmly " are caught ", reduce directly washing away of rainwater, and the area that can cover in a short time is big, and apparent effect is good;Simultaneously
Loquat has cold-resistant, drought-enduring, resistance to shade, impoverishment tolerant, the water-fast characteristic such as wet, even if planting in the sandstone that not even a blade of grass grows or slabstone gap
In, also can be flourishing, full of green, it is widely used in garden landscape and ecological recovery.In sum, ground loquat is
Integrate multi-purpose plant that is edible, medicinal, afforesting, view and admire.
Cutting propagation is a kind of asexual reproduction method being widely used in agricultural and forestry, has that reproduction speed is fast, method is simple
Single, processing ease and the neat feature of nursery stock, filial generation simultaneously can keep the excellent hereditary capacity of maternal plant.Therefore, research ground Pi
The vegetative propagation technology of rake superior genotypes, for popularization, the research of accelerating superior genotypes, sets up commercial development profit
Planting base just can make this precious resources obtain maximally effective protection and develop, and realizes the true value of its resource.
It is investigated that ask although patent (cn 103355168 a) tissue culture technique of loquat is studied over the ground, but
The method yet suffer from professional technique have high demands, the shortcoming of complex steps.Although also can be carried out using normal cutting propagation reproduction technique
Ground loquat breeding, but the time typically requiring three months about just can get the ground Loquat seedling that can use, time
Cost is of a relatively high, and floor space is big, is unfavorable for large-scale promotion.Only solve artificial rapid propagation, reduction is trained
This, expand the planting number of ground loquat, and the commercial growth application of ground loquat just can be made to become possible to.The present invention is traditional cuttage
Technology is combined with tissue culture lighting, indoors using the feature that ground loquat happiness sun happiness is wet, proper extension light application time, controls
Culture humidity, makes ground loquat fast-growth, gets, the time of short one month about, the ground Loquat seedling that can use.
Content of the invention
It is an object of the invention to provide a kind of method of cutting propagation ground loquat, overcome ground loquat generative propagation survival rate
Low problem, has using the inventive method planting site loquat that reproduction speed is fast, method simple, processing ease and nursery stock are neat
Feature, filial generation simultaneously can keep the excellent hereditary capacity of maternal plant to be capable of fast and effectively loquat vegetative propagation;And
Using the method for the present invention breeding, can more effectively protection and developmental utilization ground loquat.
For achieving the above object, the method for a kind of cutting propagation ground of the present invention loquat, comprises the following steps:
(1) take raw, healthy and strong, anosis spray then to make cutting, adopt the bar time for 4-10 month;
(2) the long 8-10cm of cuttage branch, comprises the 2-3 bud enriching, and cuttings base portion otch is splayed, and cuttings base portion leaves one
Individual axillary bud, only contains the cuttings top truncation of axillary bud, and blade all removes;Cuttings containing terminal bud, only leaves and takes 2-3 piece near terminal bud end
Launch completely blade;Finally cuttings base portion is carried out after quickly dipped 30s process in 1% liquor potassic permanganate, be dipped in through
1.5-2h in the solution of state's light root-inducing powder that 1500-2000 times dilutes, then carries out cuttage;
(3) cuttage severity control, in 2-3cm, is defined by 1 axillary bud inserting base portion, and cutting density is one plant of a cave;
(4) management after cuttage: water in time after cuttage, first three sky only waters, later daily only morning and evening sprinkling 1/2 lattice suddenly
Blue nutrient solution is until before hardening, fountain height is all so that cuttage seeding local environment relative humidity is 75-90%;Temperature is maintained at 22-
28℃;First three day light application time is 10-12h, and light application time is 20-24h later, and intensity of illumination is 2500-3000lux until refining
Before seedling;
(5) acclimatization and transplantses: when cuttage seeding is cultivated 30 days, when that is, cuttage seeding grows 5-8cm children's stem, sprinkle water air moisturizing 60-
70%, in natural light lower refining seedling 5-7 days, that is, transplant land for growing field crops.
It is dipped in the solution of the 1500-2000 times of state's light root-inducing powder diluting by 50-80 strain a bundle cuttings in step (2)
1.5-2h.
Cutting medium is made up of perlite, and matrix can recycle.
, in hole tray, 40-50 strain is placed in long * width * for the Seedling bag being made using non-woven fabrics in step (3) or cuttage
In the big basin of a height of 30cm*30cm*5cm, then it is placed on tissue culture frame.
The further detailed protocol of the present invention is as follows:
First, choose anosis harm, growth is fast, premunition is strong elite plant makees elite stand, elite stand is taken give birth to then tender
Branch makees cutting, adopts the bar time for 4-10 month, chooses robust growth, anosis branch;
2nd, the long 8-10cm of cuttage branch, comprises the 2-3 bud enriching, and cuttings base portion otch is splayed, and (open cross-sectional is ellipse
Circle), cuttings base portion leaves an axillary bud, only contains the cuttings top truncation (open cross-sectional is circle) of axillary bud, blade all goes
Remove;Cuttings containing terminal bud, only leaves and takes 2-3 piece near terminal bud end and launches completely blade;Finally by cuttings base portion in 1% permanganic acid
After carrying out quickly dipped 30s process in potassium solution, it is dipped in 1.5-2h in the solution of the 1500-2000 times of state's light root-inducing powder diluting,
Then carry out cuttage;
3rd, in hole tray, 40-50 strain is a basin, is placed in long * for the Seedling bag being made using non-woven fabrics or cuttage
In the basin of a height of 30cm*30cm*5cm of wide *, finally it is placed on tissue culture frame, matrix is made up of perlite completely.
4th, it is compacted after cuttage, cuttage severity control is defined by 1 axillary bud inserting base portion in 2-3cm, cuttings, and cuttage is close
Spend for one plant of a cave, dense planting is in cuttage basin;Management after cuttage is to water in time after cuttage, and first three sky only waters, often later
Glan nutrient solution, up to before hardening, waters or nutrient solution all makes cuttage seeding local environment relative humidity suddenly for its sprinkling 1/2 sooner or later
For 75-90%;According further to needing it is ensured that take root required ventilation, temperature and humidity condition;
5th, the managing and protecting of cuttage seeding: after the good fringe bar of cuttage, temperature is maintained at 22-28 DEG C;First three day light application time is 10-
12h, light application time is 20-24h later, and intensity of illumination is 2500-3000lux up to before hardening.
6th, acclimatization and transplantses
When cuttage seeding is cultivated 30 days about, when that is, cuttage seeding grows 5-8cm children's stem, sprinkle water moisturizing 60-70%, in natural light
Lower refining seedling 5-7 days, you can transplant land for growing field crops together with non-woven fabrics pot for growing seedlings.
Optionally adopt insecticide gardens 1, mainly kill except insect pests such as red spiders.
A kind of method of cutting propagation ground of the present invention loquat, its advantage is: the ground loquat that the present invention provides
Propagation method simple possible, low cost, cycle is short, also have very strong factorial praluction ability.It is capable of fast and effectively
Ground loquat vegetative propagation;And using the method for the present invention breeding, can more effectively protection and developmental utilization ground loquat.
Brief description
Fig. 1 grows the photo of 10 days, 20 days and 30 days for cuttings;
Fig. 2 is non-woven fabrics cultivating seedling;
Fig. 3 is Survival rate of nursery stock photo after transplanting.
Specific embodiment
Following examples are further elaborated for the present invention, without forming limitation of the present invention.
Embodiment 1
First, choose anosis harm, growth is fast, premunition is strong elite plant makees elite stand, elite stand is taken give birth to then tender
Branch makees cutting, adopts the bar time for April, chooses robust growth, anosis semi-lignified branch;
2nd, the long 8cm of cuttage branch, comprises 3 substantial axillary buds, and fringe base portion otch is splayed, and base portion leaves an axillary bud,
The truncation of cuttings top, blade all removes;Fringe base portion is carried out after quickly dipped 30s process in 1% liquor potassic permanganate, by 50 plants
A bundle is dipped in 2h in the solution of state's light root-inducing powder of 2000 times of dilutions;
3rd, carry out cuttage using non-woven fabrics Seedling bag, after the completion of cuttage, load pallet, 40 plant of one basin is finally placed in tissue culture
On frame, matrix is perlite.
4th, it is compacted after cuttage, cuttage severity control, in 2-3cm, is specifically defined by the axillary bud that submerges, cutting density is one
One plant of cave, dense planting is in cuttage basin;Management after cuttage is to water in time after cuttage, and first three sky only waters, later daily sooner or later
Spray a small amount of 1/2 suddenly Glan nutrient solution until hardening before;Water or nutrient solution all makes the cuttage seeding local environment relative humidity be
75-85%;According further to needing it is ensured that take root required ventilation, temperature and humidity condition;
5th, the managing and protecting of cuttage seeding: after the good fringe bar of cuttage, temperature is maintained at 22-28 DEG C;First three day light application time is 12h, with
Light application time is 24h afterwards, and intensity of illumination is 2500-3000lux;Until before hardening.
6th, acclimatization and transplantses
When cuttage seeding is cultivated 30 days about, when that is, cuttage seeding grows 5-8cm children's stem, sprinkle water moisturizing 60-70%, in natural light
Lower refining seedling 5-7 days, you can transplant land for growing field crops together with non-woven fabrics pot for growing seedlings.
In whole experiment process, cuttage seeding grows fine;Result shows the cutting propagation cycle from cuttage to growing 5-8cm's
Spray is 27 days, if being provided without the illumination method of the present invention, such as daily light application time is all 12 hours, until acclimatization and transplantses
Before, the seedling time will postpone nearly two months than the inventive method close to 90 days.The survival rate of statistics cuttage seeding, result shows skewer
The survival rate (total number of the cutting number/cuttage surviving) of seedlings plugging is 92% ± 1%.
Embodiment 2
First, loquat clump branch, the fine individual plant that harmful, growth is fast, premunition is strong of being critically ill make elite stand no, take and work as on elite stand
Year, raw spray made cutting, adopts the bar time for September part, chooses robust growth, anosis semi-lignified branch;
2nd, the long 8cm of cuttage branch, comprises 3 substantial buds, and fringe base portion otch is splayed, and base portion leaves an axillary bud, contains
The cuttings of 1 terminal bud is left and taken 2-3 piece near terminal bud end and is launched completely blade, and fringe base portion is entered in 1% liquor potassic permanganate
Scanning frequency is dipped in after 30s is processed and is dipped in 2h in the solution of state's light root-inducing powder of 2000 times of dilutions by 50 plants of a bundles;
3rd, carry out cuttage using hole tray, using loading pallet after the completion of cuttage, be 50 plants/every basin, be finally placed in tissue culture
On frame, matrix is perlite.
4th, it is compacted after cuttage, cuttage severity control, in 2-3cm, is specifically defined by the axillary bud that submerges, cutting density is one
One plant of cave, dense planting is in cuttage basin;Management after cuttage is to water in time after cuttage, and first three sky only waters, later daily sooner or later
Spray a small amount of 1/2 suddenly Glan nutrient solution until before hardening, watering or nutrient solution all make the cuttage seeding local environment relative humidity be
75-85%;According further to needing it is ensured that take root required ventilation, temperature and humidity condition;
5th, the managing and protecting of cuttage seeding: after the good fringe bar of cuttage, temperature is maintained at 22-28 DEG C;First three day light application time is 12h, with
Light application time is 24h afterwards, and intensity of illumination is 2500-3000lux up to before hardening.
6th, acclimatization and transplantses
When cuttage seeding is cultivated 30 days about, when that is, cuttage seeding grows 5-8cm children's stem, sprinkle water moisturizing 60-70%, in natural light
Lower refining seedling 5-7 days, you can transplant land for growing field crops together with non-woven fabrics pot for growing seedlings.
In whole experiment process, cuttage seeding grows fine;Result shows the cutting propagation cycle from cuttage to growing 5-8cm's
Spray is 35 days, if being provided without the illumination method of the present invention, such as daily light application time is all 12 hours, until acclimatization and transplantses
Before, the seedling time will postpone nearly two months than the inventive method close to 90 days.The survival rate of statistics cuttage seeding, result shows skewer
The survival rate (total number of the cutting number/cuttage surviving) of seedlings plugging is 92% ± 1%.
Claims (3)
1. a kind of method of cutting propagation ground loquat is it is characterised in that comprise the following steps:
(1) take raw, healthy and strong, anosis spray then to make cutting, adopt the bar time for 4-10 month;
(2) the long 8-10cm of cuttage branch, comprises the 2-3 bud enriching, and cuttings base portion otch is splayed, and cuttings base portion leaves an armpit
Bud, only contains the cuttings top truncation of axillary bud, and blade all removes;Cuttings containing terminal bud, only leaves and takes 2-3 piece near terminal bud end and launches
Completely blade;Finally cuttings base portion is carried out after quickly dipped 30s process in 1% liquor potassic permanganate, be dipped in through 1500-
1.5-2h in the solution of state's light root-inducing powder of 2000 times of dilutions, then carries out cuttage;
(3), in hole tray, 40-50 strain is placed in a height of 30cm* of long * width * for the Seedling bag being made using non-woven fabrics or cuttage
In the big basin of 30cm*5cm, then it is placed on tissue culture frame;Cuttage severity control, in 2-3cm, with 1 axillary bud inserting base portion is
Standard, cutting density is one plant of a cave;
(4) management after cuttage: water in time after cuttage, first three sky only waters, the later daily only morning and evening sprays 1/2 Huo Gelanying
Nutrient solution is until before hardening, fountain height is all so that cuttage seeding local environment relative humidity is 75-90%;Temperature is maintained at 22-28 DEG C;
First three day light application time is 10-12h, and light application time is 20-24h later, and intensity of illumination is 2500-3000lux up to before hardening;
(5) acclimatization and transplantses: when cuttage seeding is cultivated 30 days, when that is, cuttage seeding grows 5-8cm children's stem, sprinkle water air moisturizing 60-70%,
In natural light lower refining seedling 5-7 days, that is, transplant land for growing field crops.
2. the method for cutting propagation ground according to claim 1 loquat is it is characterised in that press 50-80 strain one in step (2)
Bundle cuttings is dipped in 1.5-2h in the solution of the 1500-2000 times of state's light root-inducing powder diluting.
3. the method for cutting propagation ground according to claim 1 loquat is it is characterised in that cutting medium is by perlite group
Become, matrix can recycle.
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Families Citing this family (6)
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CN106612695A (en) * | 2016-09-27 | 2017-05-10 | 西南科技大学 | Method for repairing mining area vegetation and soil by using Ficus tikoua bur |
CN106734182B (en) * | 2016-12-16 | 2020-05-29 | 四川省文景环保工程有限公司 | Method for promoting plants to absorb heavy metals by using plants |
CN107396801A (en) * | 2017-08-30 | 2017-11-28 | 普定县绿源苗业开发有限公司 | A kind of cuttage implantation methods of wild country melon |
CN108738772B (en) * | 2018-05-18 | 2021-03-02 | 象州县科学技术情报研究所 | Cutting seedling breeding method for schefflera octophylla |
CN109168687A (en) * | 2018-09-25 | 2019-01-11 | 上海市农业科学院 | A method of vegetative propagation is carried out using loquat stock |
CN110419429A (en) * | 2019-08-29 | 2019-11-08 | 贵州大学 | A method of promoting wild country melon cutting survival |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN103355168A (en) * | 2013-07-16 | 2013-10-23 | 中国科学院亚热带农业生态研究所 | Ficus tikoua rapid propagation method |
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CN103355168A (en) * | 2013-07-16 | 2013-10-23 | 中国科学院亚热带农业生态研究所 | Ficus tikoua rapid propagation method |
Non-Patent Citations (2)
Title |
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枇杷嫩枝扦插繁殖技术研究;徐金莲等;《现代农业科技》;20111231(第6期);120 * |
枇杷嫩枝扦插育苗研究;余亚白等;《福建农科院学报》;19911231;第6卷(第2期);66-70 * |
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