CN104604868A - Disinfectant for silkworm rearing rooms, silkworm rearing instruments and silkworm rearing beds and application of disinfectant - Google Patents
Disinfectant for silkworm rearing rooms, silkworm rearing instruments and silkworm rearing beds and application of disinfectant Download PDFInfo
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- CN104604868A CN104604868A CN201510001579.8A CN201510001579A CN104604868A CN 104604868 A CN104604868 A CN 104604868A CN 201510001579 A CN201510001579 A CN 201510001579A CN 104604868 A CN104604868 A CN 104604868A
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- bromothalonil
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Abstract
The invention relates to a disinfectant for silkworm rearing rooms, silkworm rearing instruments and silkworm rearing beds and application of the disinfectant. The disinfectant is prepared by uniformly mixing the following raw material components: boscalid, bromothalonil and water. According to the disinfectant, boscalid and the bromothalonil are firstly mixed to prepare the disinfectant for the silkworm rearing rooms, the silkworm rearing instruments and the silkworm rearing beds; the boscalid and the bromothalonil are combined to achieve a synergistic effect of killing silkworm pathogenic funguses including beauveria bassiana and aspergillus, so that application amount of the boscalid and the bromothalonil can be greatly reduced; the disinfectant can disinfect the silkworm rearing rooms and the rearing instruments, and can also be directly sprayed to the silkworms to disinfectant the silkworm rearing beds.
Description
Technical field
The present invention relates to the rearing instrument disinfection of rearing bed agent of a kind of silkworm room and application thereof, belong to silkworm disease prevention techniques field.
Background technology
China is quality raw materials cocoon production base, the world, and export volume accounts for more than 80% of the world.The direct economic loss caused because of flacherie in producing of breeding silkworms accounts for 10 ~ 15% of total income, and silkworm fungal disease is the Major Diseases during silk cocoon is produced, and the generation of disease is produced to silk cocoon and brought very large loss.
Prior art widely uses the aldehyde preparations such as the chlorinated product such as bleaching powder, DCCA and formaldehyde.The research of recent two decades uses and shows, the harm that chlorinated product and aldehyde preparation cause environment and health is very huge, all form grave danger to the nervous system such as the mankind and animal is healthy, very large pollution and destruction are caused to environment, therefore prohibits the use in developed country already.Continue a large amount of chlorinated product and aldehyde preparation of using and be extremely unfavorable for sustainable development.
Bromothalonil, bromo-2, the 4-dicyanobutanes of chemical name: 1,2'-bis-, molecular formula: C
6h
6br
2n
2, molecular weight: 265.94.Bromothalonil can be absorbed by somatic cells rapidly, and the normal development of Conduction Interference somatic cells in somatic cells, thus reach antibacterial, bactericidal action.Bromothalonil in test dose to animal without teratogenesis, mutagenesis, carcinogenesis, to people, animal, ecological environment security.
Boscalid, the chloro-N-of chemical name: 2-(4 '-chlorodiphenyl-2-base) vitamin PP, molecular formula: C
18h
12c
l2n
2o, molecular weight: 343.21.Boscalid belongs to succinic acid ubiquinone reductase inhibitor in mitochondrial respiratory chain, suppresses mitochondrial succinate acid esters dehydrase, hinders tricarbonic acid cycle, makes amino acid, sugar lacks, energy reduces, the division of interference cell and growth and make thalline dead.Boscalid stops fungi production capacity by Profilin matter complex, thus Antifungi growth, and with other bactericide without cross resistance.Rat acute per os LD
50>5000mg/kg, to rabbit eye irritation, to rabbit no skin irritation, belongs to the environmentally friendly bactericide to people, animal, ecological environment security.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, the rearing instrument disinfection of rearing bed agent of a kind of silkworm room and application are provided.
Technical solution of the present invention is as follows:
A kind of silkworm room rearing instrument disinfection of rearing bed agent, raw material components is as follows, is weight portion:
Boscalid 2 parts, bromothalonil 1 ~ 20 part, 50 ~ 4000 parts, water; Above-mentioned raw materials component is mixed and get final product.
Preferred according to the present invention, Boscalid 2 parts, bromothalonil 5 ~ 20 parts, 100 ~ 2000 parts, water.
The application of above-mentioned silkworm room rearing instrument disinfection of rearing bed agent in the rearing instrument disinfection of rearing bed of silkworm room.
Above-mentioned application, silkworm room disinfection of tools for silkworm rearing step is as follows:
By silkworm room rearing instrument disinfection of rearing bed agent by 200 ~ 300ml/m
2the amount of spraying spray impregnated in 15 ~ 30min in this disinfectant on the rearing instrument of silkworm room or by rearing instrument.
Above-mentioned application, disinfection of rearing bed step is as follows:
By silkworm room rearing instrument disinfection of rearing bed agent by 200 ~ 300ml/m
2the amount of spraying spray on silkworm rearing bed, silkworm body.
The present invention has following beneficial effect:
1, the present invention prepares silkworm room rearing instrument disinfection of rearing bed agent after being mixed with bromothalonil by Boscalid first, Boscalid and bromothalonil conbined usage kill white muscardine fungi, Aspergillus has collaborative facilitation interior silkworm pathomycete, significantly can reduce the amount of application of the two, both to silkworm room disinfection of tools for silkworm rearing, silkworm body can also can directly be sprayed to disinfection of rearing bed;
2, this disinfectant is safe and efficient, to animal without teratogenesis, mutagenesis, carcinogenesis, belongs to the environmentally friendly bactericide to people, animal, ecological environment security, is conducive to the protection of the medicine person of spraying self;
3, this product application process is simple, is conducive to applying on a large scale.
Embodiment
Below in conjunction with embodiment, the present invention is further elaborated, but institute of the present invention protection domain is not limited thereto.
Described in embodiment, Boscalid is purchased from Basf China Co., Ltd, and bromothalonil is purchased from Jiangsu Tuoqiu Agrochemicals Co., Ltd..
Embodiment 1
A kind of silkworm room rearing instrument disinfection of rearing bed agent, raw material components is as follows, is weight portion:
Boscalid 2g, bromothalonil 5g, water 1000g; By Boscalid and bromothalonil mixing soluble in water and get final product.
Following experiment is done to the Disinfection Effect of the silkworm room rearing instrument disinfection of rearing bed agent that embodiment 1 obtains:
A, white muscardine fungi test
Medication group:
By white muscardine fungi bacterium liquid (bacterial concentration: 5.0 × 10
7individual/ml) 200 microlitres mix with silkworm room rearing instrument disinfection of rearing bed agent 200 microlitre, be coated with and play silkworm body surface 2 ages to moistening after 30 minutes, and culture dish lid is included to be dipped in after the moisturizing of water filter paper raises 24 hours, changes into and normally raising;
Control group:
By white muscardine fungi bacterium liquid (bacterial concentration: 5.0 × 10
7individual/ml) 200 microlitres mix with running water 200 microlitre, be coated with and play silkworm body surface 2 ages to moistening after 30 minutes, and culture dish lid is included to be dipped in after the moisturizing of water filter paper raises 24 hours, changes into and normally raising;
Raise two the length of time " Invest, Then Investigate " survival rate.
B, Aspergillus are tested
Medication group:
By Aspergillus bacterium liquid (bacterial concentration: 5.0 × 10
7individual/ml) 200 microlitres mix with silkworm room rearing instrument disinfection of rearing bed agent 200 microlitre, are coated with newly-hatched silkworm body surface to moistening after 30 minutes, and culture dish lid is included to be dipped in after the moisturizing of water filter paper raises 24 hours, changes into and normally raising;
Control group:
By Aspergillus bacterium liquid (bacterial concentration: 5.0 × 10
7individual/ml) 200 microlitres mix with running water 200 microlitre, are coated with newly-hatched silkworm body surface to moistening after 30 minutes, and culture dish lid is included to be dipped in after the moisturizing of water filter paper raises 24 hours, changes into and normally raising;
Raise two the length of time " Invest, Then Investigate " survival rate.
Table 1
| Sequence number | Silkworm quantity | Cause of disease | Administrated method | Responding time | Silkworm survival rate (%) |
| (head) | (minute) | Medication group | Control group | |||
| a | 98 | White muscardine fungi | Silkworm body is coated with after germ sterilization | 30 | 100 | 0 |
| b | 102 | Aspergillus | Silkworm body is coated with after germ sterilization | 30 | 100 | 0 |
Embodiment 2
A kind of silkworm room rearing instrument disinfection of rearing bed agent, raw material components is as follows, is weight portion:
Boscalid 2g, bromothalonil 10g, water 1500g; By Boscalid and bromothalonil mixing soluble in water and get final product.
Following experiment is done to the Disinfection Effect of the silkworm room rearing instrument disinfection of rearing bed agent that embodiment 2 obtains:
A, white muscardine fungi test
Medication group:
By white muscardine fungi bacterium liquid (bacterial concentration: 5.0 × 10
7individual/ml) 200 microlitres mix with silkworm room rearing instrument disinfection of rearing bed agent 200 microlitre, be coated with and play silkworm body surface 2 ages to moistening after 20 minutes, and culture dish lid is included to be dipped in after the moisturizing of water filter paper raises 24 hours, changes into and normally raising;
Control group:
By white muscardine fungi bacterium liquid (bacterial concentration: 5.0 × 10
7individual/ml) 200 microlitres mix with running water 200 microlitre, be coated with and play silkworm body surface 2 ages to moistening after 20 minutes, and culture dish lid is included to be dipped in after the moisturizing of water filter paper raises 24 hours, changes into and normally raising;
Raise two the length of time " Invest, Then Investigate " survival rate.
B, Aspergillus are tested
Medication group:
By Aspergillus bacterium liquid (bacterial concentration: 5.0 × 10
7individual/ml) 200 microlitres mix with silkworm room rearing instrument disinfection of rearing bed agent 200 microlitre, are coated with newly-hatched silkworm body surface to moistening after 20 minutes, and culture dish lid is included to be dipped in after the moisturizing of water filter paper raises 24 hours, changes into and normally raising;
Control group:
By Aspergillus bacterium liquid (bacterial concentration: 5.0 × 10
7individual/ml) 200 microlitres mix with running water 200 microlitre, are coated with newly-hatched silkworm body surface to moistening after 20 minutes, and culture dish lid is included to be dipped in after the moisturizing of water filter paper raises 24 hours, changes into and normally raising;
Raise two the length of time " Invest, Then Investigate " survival rate.
Table 2
Embodiment 3
A kind of silkworm room rearing instrument disinfection of rearing bed agent, raw material components is as follows, is weight portion:
Boscalid 2g, bromothalonil 9g, water 800g; By Boscalid and bromothalonil mixing soluble in water and get final product.
Following experiment is done to the Disinfection Effect of the silkworm room rearing instrument disinfection of rearing bed agent that embodiment 3 obtains:
A, white muscardine fungi test
Medication group:
By white muscardine fungi bacterium liquid (bacterial concentration: 5.0 × 10
7individual/ml) 200 microlitres mix with silkworm room rearing instrument disinfection of rearing bed agent 200 microlitre, be coated with and play silkworm body surface 2 ages to moistening after 15 minutes, and culture dish lid is included to be dipped in after the moisturizing of water filter paper raises 24 hours, changes into and normally raising;
Control group:
By white muscardine fungi bacterium liquid (bacterial concentration: 5.0 × 10
7individual/ml) 200 microlitres mix with running water 200 microlitre, be coated with and play silkworm body surface 2 ages to moistening after 15 minutes, and culture dish lid is included to be dipped in after the moisturizing of water filter paper raises 24 hours, changes into and normally raising;
Raise two the length of time " Invest, Then Investigate " survival rate.
B, Aspergillus are tested
Medication group:
By Aspergillus bacterium liquid (bacterial concentration: 5.0 × 10
7individual/ml) 200 microlitres mix with silkworm room rearing instrument disinfection of rearing bed agent 200 microlitre, are coated with newly-hatched silkworm body surface to moistening after 15 minutes, and culture dish lid is included to be dipped in after the moisturizing of water filter paper raises 24 hours, changes into and normally raising;
Control group:
By Aspergillus bacterium liquid (bacterial concentration: 5.0 × 10
7individual/ml) 200 microlitres mix with running water 200 microlitre, are coated with newly-hatched silkworm body surface to moistening after 15 minutes, and culture dish lid is included to be dipped in after the moisturizing of water filter paper raises 24 hours, changes into and normally raising;
Raise two the length of time " Invest, Then Investigate " survival rate.
Table 3
Embodiment 4
A kind of silkworm room rearing instrument disinfection of rearing bed agent, raw material components is as follows, is weight portion:
Boscalid 2g, bromothalonil 15g, water 1800g; By Boscalid and bromothalonil mixing soluble in water and get final product.
Following experiment is done to the Disinfection Effect of the silkworm room rearing instrument disinfection of rearing bed agent that embodiment 4 obtains:
A, white muscardine fungi test
Medication group:
By white muscardine fungi bacterium liquid (bacterial concentration: 5.0 × 10
7individual/ml) 200 microlitres mix with silkworm room rearing instrument disinfection of rearing bed agent 200 microlitre, be coated with and play silkworm body surface 2 ages to moistening after 20 minutes, and culture dish lid is included to be dipped in after the moisturizing of water filter paper raises 24 hours, changes into and normally raising;
Control group:
By white muscardine fungi bacterium liquid (bacterial concentration: 5.0 × 10
7individual/ml) 200 microlitres mix with running water 200 microlitre, be coated with and play silkworm body surface 2 ages to moistening after 20 minutes, and culture dish lid is included to be dipped in after the moisturizing of water filter paper raises 24 hours, changes into and normally raising;
Raise two the length of time " Invest, Then Investigate " survival rate.
B, Aspergillus are tested
Medication group:
By Aspergillus bacterium liquid (bacterial concentration: 5.0 × 10
7individual/ml) 200 microlitres mix with silkworm room rearing instrument disinfection of rearing bed agent 200 microlitre, are coated with newly-hatched silkworm body surface to moistening after 20 minutes, and culture dish lid is included to be dipped in after the moisturizing of water filter paper raises 24 hours, changes into and normally raising;
Control group:
By Aspergillus bacterium liquid (bacterial concentration: 5.0 × 10
7individual/ml) 200 microlitres mix with running water 200 microlitre, are coated with newly-hatched silkworm body surface to moistening after 20 minutes, and culture dish lid is included to be dipped in after the moisturizing of water filter paper raises 24 hours, changes into and normally raising;
Raise two the length of time " Invest, Then Investigate " survival rate.
Table 4
Embodiment 5
A kind of silkworm room rearing instrument disinfection of rearing bed agent, raw material components is as follows, is weight portion:
Boscalid 2g, bromothalonil 20g, water 1200g; By Boscalid and bromothalonil mixing soluble in water and get final product.
Following experiment is done to the Disinfection Effect of the silkworm room rearing instrument disinfection of rearing bed agent that embodiment 5 obtains:
A, white muscardine fungi test
Medication group:
By white muscardine fungi bacterium liquid (bacterial concentration: 5.0 × 10
7individual/ml) 200 microlitres mix with silkworm room rearing instrument disinfection of rearing bed agent 200 microlitre, be coated with and play silkworm body surface 2 ages to moistening after 15 minutes, and culture dish lid is included to be dipped in after the moisturizing of water filter paper raises 24 hours, changes into and normally raising;
Control group:
By white muscardine fungi bacterium liquid (bacterial concentration: 5.0 × 10
7individual/ml) 200 microlitres mix with running water 200 microlitre, be coated with and play silkworm body surface 2 ages to moistening after 15 minutes, and culture dish lid is included to be dipped in after the moisturizing of water filter paper raises 24 hours, changes into and normally raising;
Raise two the length of time " Invest, Then Investigate " survival rate.
B, Aspergillus are tested
Medication group:
By Aspergillus bacterium liquid (bacterial concentration: 5.0 × 10
7individual/ml) 200 microlitres mix with silkworm room rearing instrument disinfection of rearing bed agent 200 microlitre, are coated with newly-hatched silkworm body surface to moistening after 15 minutes, and culture dish lid is included to be dipped in after the moisturizing of water filter paper raises 24 hours, changes into and normally raising;
Control group:
By Aspergillus bacterium liquid (bacterial concentration: 5.0 × 10
7individual/ml) 200 microlitres mix with running water 200 microlitre, are coated with newly-hatched silkworm body surface to moistening after 15 minutes, and culture dish lid is included to be dipped in after the moisturizing of water filter paper raises 24 hours, changes into and normally raising;
Raise two the length of time " Invest, Then Investigate " survival rate.
Table 5
Embodiment 6
A kind of silkworm room rearing instrument disinfection of rearing bed agent, raw material components is as follows, is weight portion:
Boscalid 2g, bromothalonil 1g, water 1000g; By Boscalid and bromothalonil mixing soluble in water and get final product.
Following experiment is done to the Disinfection Effect of the silkworm room rearing instrument disinfection of rearing bed agent that embodiment 1 obtains:
A, white muscardine fungi test
Medication group:
By white muscardine fungi bacterium liquid (bacterial concentration: 5.0 × 10
7individual/ml) 200 microlitres mix with silkworm room rearing instrument disinfection of rearing bed agent 200 microlitre, be coated with and play silkworm body surface 2 ages to moistening after 30 minutes, and culture dish lid is included to be dipped in after the moisturizing of water filter paper raises 24 hours, changes into and normally raising;
Control group:
By white muscardine fungi bacterium liquid (bacterial concentration: 5.0 × 10
7individual/ml) 200 microlitres mix with running water 200 microlitre, be coated with and play silkworm body surface 2 ages to moistening after 30 minutes, and culture dish lid is included to be dipped in after the moisturizing of water filter paper raises 24 hours, changes into and normally raising;
Raise two the length of time " Invest, Then Investigate " survival rate.
B, Aspergillus are tested
Medication group:
By Aspergillus bacterium liquid (bacterial concentration: 5.0 × 10
7individual/ml) 200 microlitres mix with silkworm room rearing instrument disinfection of rearing bed agent 200 microlitre, are coated with newly-hatched silkworm body surface to moistening after 30 minutes, and culture dish lid is included to be dipped in after the moisturizing of water filter paper raises 24 hours, changes into and normally raising;
Control group:
By Aspergillus bacterium liquid (bacterial concentration: 5.0 × 10
7individual/ml) 200 microlitres mix with running water 200 microlitre, are coated with newly-hatched silkworm body surface to moistening after 30 minutes, and culture dish lid is included to be dipped in after the moisturizing of water filter paper raises 24 hours, changes into and normally raising;
Raise two the length of time " Invest, Then Investigate " survival rate.
Table 6
Embodiment 7
Boscalid promotes that bromothalonil kills white muscardine fungi test
Dispenser group:
Experimental group 1: preparation is the solution of the bromothalonil of 0.25% containing mass concentration;
Experimental group 2: preparation is the solution of the bromothalonil of 0.125% containing mass concentration;
Experimental group 3: preparation is the solution of the Boscalid of 0.4% containing mass concentration;
Experimental group 4: preparation is the solution of the Boscalid of 0.2% containing mass concentration;
Experimental group 5: preparation is the solution of 0.125% bromothalonil and 0.4% Boscalid containing mass concentration;
Experimental group 6: preparation is the solution of 0.125% bromothalonil and 0.2% Boscalid containing mass concentration;
Experimental technique
By white muscardine fungi bacterium liquid (bacterial concentration: 5.0 × 10
7individual/ml) 200 microlitres mix with solution 200 microlitre that experimental group is prepared, be coated with after 15 minutes and play silkworm body surface 2 ages to moistening, culture dish lid is included to be dipped in after the moisturizing of water filter paper raises 24 hours, changes into and normally raising, raise two the length of time " Invest, Then Investigate " survival rate, by the following method calculate:
Computing formula
Result of calculation is as shown in table 7;
Table 7 Boscalid promotes that bromothalonil kills white muscardine fungi test
| Group | Silkworm survival rate % |
| Experimental group 1 | 100 |
| Experimental group 2 | 2.5 |
| Experimental group 3 | 0 |
| Experimental group 4 | 0 |
| Experimental group 5 | 52.5 |
| Experimental group 6 | 27.5 |
Embodiment 8
Bromothalonil promotes that Boscalid kills Aspergillus test
Dispenser group:
Experimental group 1: preparation is the solution of the Boscalid of 0.25% containing mass concentration;
Experimental group 2: preparation is the solution of the Boscalid of 0.1% containing mass concentration;
Experimental group 3: preparation is the solution of the bromothalonil of 1.0% containing mass concentration;
Experimental group 4: preparation is the solution of the bromothalonil of 0.1% containing mass concentration;
Experimental group 5: preparation is the solution of 0.1% Boscalid and 1.0% bromothalonil containing mass concentration;
Experimental group 6: preparation is the solution of 0.1% Boscalid and 0.1% bromothalonil containing mass concentration;
Experimental technique
By Aspergillus bacterium liquid (bacterial concentration: 5.0 × 10
7individual/ml) 200 microlitres mix with solution 200 microlitre that experimental group is prepared, be coated with after 15 minutes and play silkworm body surface 2 ages to moistening, culture dish lid is included to be dipped in after the moisturizing of water filter paper raises 24 hours, changes into and normally raising, raise two the length of time " Invest, Then Investigate " survival rate, by the following method calculate:
Computing formula
Result of calculation is as shown in table 8;
Table 8 bromothalonil promotes that Boscalid kills Aspergillus test
| Different pharmaceutical proportioning | Silkworm survival rate % |
| Experimental group 1 | 100 |
| Experimental group 2 | 65 |
| Experimental group 3 | 5 |
| Experimental group 4 | 0 |
| Experimental group 5 | 100 |
| Experimental group 6 | 100 |
Can be found out by the data of table 7 and table 8, bromothalonil has good control efficiency to the mycosis that silkworm is caused by white muscardine fungi, but it is to poor by aspergillus microbial mycosis control efficiency; Boscalid has good control efficiency to by the microbial mycosis of silkworm aspergillus, but it does not have control efficiency to the mycosis caused by white muscardine fungi.Both carry out composite by the present invention according to a certain percentage, not only can prevent and treat by white muscardine fungi and the microbial fungal disease of aspergillus through composite medicine simultaneously, and also have synergy preferably each other, significantly can reduce the amount of application of the two.
Claims (5)
1. a silkworm room rearing instrument disinfection of rearing bed agent, it is characterized in that, raw material components is as follows, is weight portion:
Boscalid 2 parts, bromothalonil 1 ~ 20 part, 50 ~ 4000 parts, water; Above-mentioned raw materials component is mixed and get final product.
2. silkworm room rearing instrument disinfection of rearing bed agent as claimed in claim 1, it is characterized in that, raw material components is as follows, is weight portion:
Boscalid 2 parts, bromothalonil 5 ~ 20 parts, 100 ~ 2000 parts, water.
3. the application of silkworm room rearing instrument disinfection of rearing bed agent according to claim 1 in the rearing instrument disinfection of rearing bed of silkworm room.
4. apply as claimed in claim 3, it is characterized in that, silkworm room disinfection of tools for silkworm rearing step is as follows:
By silkworm room rearing instrument disinfection of rearing bed agent by 200 ~ 300ml/m
2the amount of spraying spray impregnated in 15 ~ 30min in this disinfectant on the rearing instrument of silkworm room or by rearing instrument.
5. apply as claimed in claim 3, it is characterized in that, disinfection of rearing bed step is as follows:
By silkworm room rearing instrument disinfection of rearing bed agent by 200 ~ 300ml/m
2the amount of spraying spray on silkworm rearing bed, silkworm body.
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Cited By (1)
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|---|---|---|---|---|
| CN105325413A (en) * | 2015-10-28 | 2016-02-17 | 广东中迅农科股份有限公司 | Bactericidal composition containing mandipropamid and bromothalonil |
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| CN101077076A (en) * | 2007-07-06 | 2007-11-28 | 潘荣 | Disinfecting agent for rearing house, silkworm tool, silkworm body, silkworm seat and mulberry leaves |
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| CN104604868B (en) | 2016-07-06 |
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