CN102812957A - Smoking disinfectant for silkworms, preparation method and application thereof - Google Patents
Smoking disinfectant for silkworms, preparation method and application thereof Download PDFInfo
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- CN102812957A CN102812957A CN2012102720455A CN201210272045A CN102812957A CN 102812957 A CN102812957 A CN 102812957A CN 2012102720455 A CN2012102720455 A CN 2012102720455A CN 201210272045 A CN201210272045 A CN 201210272045A CN 102812957 A CN102812957 A CN 102812957A
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Abstract
The invention relates to a smoking disinfectant for silkworms, a preparation method and application of the smoking disinfectant, wherein the smoking disinfectant comprises following materials by weight: 1-20 parts of chlorobromoisocyanaria acid and 1-5 parts of combustion improver; the smoking disinfectant for silkworms is convenient to use, has no smoking sterilizing dead angle and does not increase environment humidity; the application method of the product is simple and low in cost, thus being beneficial to being popularized widely.
Description
Technical field
The present invention relates to a kind of smoking disinfectant for silkworm, preparation method and application, belong to silkworm disease control technical field.
Background technology
China is quality raw materials cocoon production base, the world, and export volume accounts for more than 80% of the world.The direct economic loss that sericulture causes because of stiff disease in producing accounts for 5%～10% of total income, and production has brought very big loss to silk cocoon in the generation of disease.
The mixture of the widely-used dichlord isocyanurice acid of prior art or sym-closene and other raw materials, the disinfectant of processing, the main component of this disinfectant is chlorinated product.The research of recent two decades is used and is shown; The organochlorine preparation is very huge to the harm that environment and health cause; All constitute grave danger to nervous systems such as the mankind and animal are healthy, environment is caused very big pollution and destruction, therefore ban use of in developed country already.Continue a large amount of chlorinated products that use and extremely be unfavorable for sustainable development.
Many in the world now countries all have clear and definite environmental protection standard that the use of chlorine-containing disinfectant is limited, and substituting chlorinated product with the bromine preparation is a global research topic, and it is significant.But from present China sterilization industry; The bromine preparation can't substitute chlorinated product in a short time, and is main because the chlorinated product cost is low, with low content of technology, manufacturing process is simple, requires technology high and produce the bromine preparation; Also to drop into certain R&D costs simultaneously, cause bromine preparation price generally higher.And now the silkworm of China already produces and exists labour intensity big, and flacherie harm is comparatively serious, and particularly Silkworm Cocoon Market is influenced greatly by international environment; Silkworm raiser's characteristics such as profit is low of breeding silkworms; If substitute chlorinated product with pure bromine preparation, under existence conditions, can reduce silkworm raiser's profit greatly, influence silkworm and already produce.Therefore, develop a kind of new low toxicity now, environmental protection, economic silkworm is just particularly important with bromine-containing disinfectant.
Summary of the invention
Technical problem to be solved by this invention provides a kind of smoking disinfectant for silkworm, preparation method and application, and this disinfectant economy, environmental protection are pollution-free.
The technical scheme that the present invention solves the problems of the technologies described above is following: a kind of smoking disinfectant for silkworm, form by following raw material: chlorobromide isocyanuric acid 1～20 weight portion, combustion adjuvant 1～5 weight portion;
The invention has the beneficial effects as follows: chlorobromide isocyanuric acid is the low toxicity chemical substance, and it is efficient to have a low toxicity, is prone to degraded, the characteristics of environmentally safe, and it is little to the toxic and side effect of silkworm body to use the back, helps the medicine person's of spraying self protection; Smoking disinfectant is easy to use, and the no dead angle of smog sterilization can not increase ambient humidity; This product application process is simple, and cost is low, helps applying on a large scale.
Chlorobromide isocyanuric acid because it is decomposed into hypobromous acid, hypochlorous acid and the acid of cyanogen urea, has the strong ability of killing fungi.The chlorobromide isocyanuric acid chemical property is stable, is convenient to store transportation; Safe in utilization, easy, consumption is few, duration of efficacy is long.The significant damage that environment and health is caused with respect to simple chlorinated product; Chlorobromide isocyanuric acid belongs to the low toxicity bactericide, to the skin nonirritant, does not change the somatic chromosome integrality; Do not have the effect that causes gene mutation, and cost only is equivalent to 50% of pure bromine preparation C5H6Br2N2O2.
On the basis of technique scheme, the present invention can also do following improvement.
Further, said chlorobromide isocyanuric acid is 2～4 weight portions;
Further, said combustion adjuvant is any one in flour, wood dust, the carbon dust, is preferably flour;
The present invention also provides a kind of aforesaid smoking disinfectant for silkworm to culture the application in the diseases prevention at silkworm room disinfection of tools for silkworm rearing and/or silkworm.
The present invention also provides a kind of preparation method of smoking disinfectant for silkworm, comprising: with the chlorobromide isocyanuric acid of 1～20 weight portion and the combustion adjuvant of 1～5 weight portion, packing promptly gets respectively; Before using chlorobromide isocyanuric acid and combustion adjuvant are mixed, light and be fuming.
Embodiment
Below principle of the present invention and characteristic are described, institute gives an actual example and only is used to explain the present invention, is not to be used to limit scope of the present invention.
Chlorobromide isocyanuric acid described in the embodiment is liked agricultural chemical industry Co., Ltd available from western Shandong, and flour is available from the new cigarette in Yantai Food Co., Ltd.
Embodiment 1
Chlorobromide isocyanuric acid: 30g, flour: 10g; Chlorobromide isocyanuric acid and flour mixing are promptly got.
In chlorobromide isocyanuric acid, at every cubic metre of medication 2g of confined space, disinfecting time is 60min.
Do following experiment in the face of the Disinfection Effect of this disinfectant down:
A, white muscardine fungi test
The medication group:
With white muscardine fungi bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) 100 microlitres place on the foursquare sheet glass of 2cm * 2cm, smear evenly, dry naturally.After sheet glass being put into the cupboard Smudging sterilizing 60min of sealing, sheet glass taken out add water 100 microlitres, has wiped with cotton balls and liquid has been coated with to play the silkworm body surface 2 ages extremely moistening, culture dish cover built-in dip in water filter paper preserve moisture raise 24 hours after, change into normally and raising;
Control group:
With white muscardine fungi bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) 100 microlitres place on the foursquare sheet glass of 2cm * 2cm, smear evenly, dry naturally.On sheet glass, add water 100 microlitres, wiped liquid is coated with cotton balls and played the silkworm body surface 2 ages to moistening, culture dish lid built-in dip in water filter paper preserve moisture raise 24 hours after, change into normally and raising;
This experiment raising " Invest, Then Investigate " incidence of disease in two length of time.
B, Aspergillus test
The medication group:
With Aspergillus bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) 100 microlitres place on the foursquare sheet glass of 2cm * 2cm, smear evenly, dry naturally.After sheet glass being put into the cupboard Smudging sterilizing 60min of sealing, sheet glass taken out add water 100 microlitres, has wiped with cotton balls that liquid is coated with the newly-hatched silkworm body surface is extremely moistening, culture dish cover built-in dip in water filter paper preserve moisture raise 24 hours after, change into normally and raising;
Control group:
With Aspergillus bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) 100 microlitres place on the foursquare sheet glass of 2cm * 2cm, smear evenly, dry naturally.On sheet glass, add water 100 microlitres, wiped with cotton balls liquid is coated with the newly-hatched silkworm body surface to moistening, culture dish lid built-in dip in water filter paper preserve moisture raise 24 hours after, change into normally and raising;
This experiment raising " Invest, Then Investigate " incidence of disease in two length of time.
Experimental result is as shown in table 1:
Table 1
Embodiment 2
Chlorobromide isocyanuric acid: 30g, flour: 10g; Chlorobromide isocyanuric acid and flour mixing are promptly got.
In chlorobromide isocyanuric acid, at every cubic metre of medication 5g of confined space, disinfecting time is 30min.
Do following experiment in the face of the Disinfection Effect of this disinfectant down:
A, white muscardine fungi test
The medication group:
With white muscardine fungi bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) 100 microlitres place on the foursquare sheet glass of 2cm * 2cm, smear evenly, dry naturally.After sheet glass being put into the cupboard Smudging sterilizing 30min of sealing, sheet glass taken out add water 100 microlitres, has wiped with cotton balls and liquid has been coated with to play the silkworm body surface 2 ages extremely moistening, culture dish cover built-in dip in water filter paper preserve moisture raise 24 hours after, change into normally and raising;
Control group:
With white muscardine fungi bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) 100 microlitres place on the foursquare sheet glass of 2cm * 2cm, smear evenly, dry naturally.On sheet glass, add water 100 microlitres, wiped liquid is coated with cotton balls and played the silkworm body surface 2 ages to moistening, culture dish lid built-in dip in water filter paper preserve moisture raise 24 hours after, change into normally and raising;
This experiment raising " Invest, Then Investigate " incidence of disease in two length of time.
B, Aspergillus test
The medication group:
With Aspergillus bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) 100 microlitres place on the foursquare sheet glass of 2cm * 2cm, smear evenly, dry naturally.After sheet glass being put into the cupboard Smudging sterilizing 30min of sealing, sheet glass taken out add water 100 microlitres, has wiped with cotton balls that liquid is coated with the newly-hatched silkworm body surface is extremely moistening, culture dish cover built-in dip in water filter paper preserve moisture raise 24 hours after, change into normally and raising;
Control group:
With Aspergillus bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) 100 microlitres place on the foursquare sheet glass of 2cm * 2cm, smear evenly, dry naturally.On sheet glass, add water 100 microlitres, wiped with cotton balls liquid is coated with the newly-hatched silkworm body surface to moistening, culture dish lid built-in dip in water filter paper preserve moisture raise 24 hours after, change into normally and raising;
This experiment raising " Invest, Then Investigate " incidence of disease in two length of time.
Experimental result is as shown in table 2:
Table 2
Embodiment 3
Chlorobromide isocyanuric acid: 2g, flour: 2g; Chlorobromide isocyanuric acid and flour mixing are promptly got.
In chlorobromide isocyanuric acid, at every cubic metre of medication 2g of confined space, disinfecting time is 60min.
Do following experiment in the face of the Disinfection Effect of this disinfectant down:
A, white muscardine fungi test
The medication group:
With white muscardine fungi bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) 100 microlitres place on the foursquare sheet glass of 2cm * 2cm, smear evenly, dry naturally.After sheet glass being put into the cupboard Smudging sterilizing 60min of sealing, sheet glass taken out add water 100 microlitres, has wiped with cotton balls and liquid has been coated with to play the silkworm body surface 2 ages extremely moistening, culture dish cover built-in dip in water filter paper preserve moisture raise 24 hours after, change into normally and raising;
Control group:
With white muscardine fungi bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) 100 microlitres place on the foursquare sheet glass of 2cm * 2cm, smear evenly, dry naturally.On sheet glass, add water 100 microlitres, wiped liquid is coated with cotton balls and played the silkworm body surface 2 ages to moistening, culture dish lid built-in dip in water filter paper preserve moisture raise 24 hours after, change into normally and raising;
This experiment raising " Invest, Then Investigate " incidence of disease in two length of time.
B, Aspergillus test
The medication group:
With Aspergillus bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) 100 microlitres place on the foursquare sheet glass of 2cm * 2cm, smear evenly, dry naturally.After sheet glass being put into the cupboard Smudging sterilizing 60min of sealing, sheet glass taken out add water 100 microlitres, has wiped with cotton balls that liquid is coated with the newly-hatched silkworm body surface is extremely moistening, culture dish cover built-in dip in water filter paper preserve moisture raise 24 hours after, change into normally and raising;
Control group:
With Aspergillus bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) 100 microlitres place on the foursquare sheet glass of 2cm * 2cm, smear evenly, dry naturally.On sheet glass, add water 100 microlitres, wiped with cotton balls liquid is coated with the newly-hatched silkworm body surface to moistening, culture dish lid built-in dip in water filter paper preserve moisture raise 24 hours after, change into normally and raising;
This experiment raising " Invest, Then Investigate " incidence of disease in two length of time.
Experimental result is as shown in table 3:
Table 3
Embodiment 4
Chlorobromide isocyanuric acid: 40g, flour: 10g; Chlorobromide isocyanuric acid and flour mixing are promptly got.
In chlorobromide isocyanuric acid, at every cubic metre of medication 5g of confined space, disinfecting time is 30min.
Do following experiment in the face of the Disinfection Effect of this disinfectant down:
A, white muscardine fungi test
The medication group:
With white muscardine fungi bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) 100 microlitres place on the foursquare sheet glass of 2cm * 2cm, smear evenly, dry naturally.After sheet glass being put into the cupboard Smudging sterilizing 30min of sealing, sheet glass taken out add water 100 microlitres, has wiped with cotton balls and liquid has been coated with to play the silkworm body surface 2 ages extremely moistening, culture dish cover built-in dip in water filter paper preserve moisture raise 24 hours after, change into normally and raising;
Control group:
With white muscardine fungi bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) 100 microlitres place on the foursquare sheet glass of 2cm * 2cm, smear evenly, dry naturally.On sheet glass, add water 100 microlitres, wiped liquid is coated with cotton balls and played the silkworm body surface 2 ages to moistening, culture dish lid built-in dip in water filter paper preserve moisture raise 24 hours after, change into normally and raising;
This experiment raising " Invest, Then Investigate " incidence of disease in two length of time.
B, Aspergillus test
The medication group:
With Aspergillus bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) 100 microlitres place on the foursquare sheet glass of 2cm * 2cm, smear evenly, dry naturally.After sheet glass being put into the cupboard Smudging sterilizing 30min of sealing, sheet glass taken out add water 100 microlitres, has wiped with cotton balls that liquid is coated with the newly-hatched silkworm body surface is extremely moistening, culture dish cover built-in dip in water filter paper preserve moisture raise 24 hours after, change into normally and raising;
Control group:
With Aspergillus bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) 100 microlitres place on the foursquare sheet glass of 2cm * 2cm, smear evenly, dry naturally.On sheet glass, add water 100 microlitres, wiped with cotton balls liquid is coated with the newly-hatched silkworm body surface to moistening, culture dish lid built-in dip in water filter paper preserve moisture raise 24 hours after, change into normally and raising;
This experiment raising " Invest, Then Investigate " incidence of disease in two length of time.
Experimental result is as shown in table 4:
Table 4
Embodiment 5
Chlorobromide isocyanuric acid: 20g, flour: 7g; Chlorobromide isocyanuric acid and flour mixing are promptly got.
In chlorobromide isocyanuric acid, at every cubic metre of medication 2g of confined space, disinfecting time is 60min.
Do following experiment in the face of the Disinfection Effect of this disinfectant down:
A, white muscardine fungi test
The medication group:
With white muscardine fungi bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) 100 microlitres place on the foursquare sheet glass of 2cm * 2cm, smear evenly, dry naturally.After sheet glass being put into the cupboard Smudging sterilizing 60min of sealing, sheet glass taken out add water 100 microlitres, has wiped with cotton balls and liquid has been coated with to play the silkworm body surface 2 ages extremely moistening, culture dish cover built-in dip in water filter paper preserve moisture raise 24 hours after, change into normally and raising;
Control group:
With white muscardine fungi bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) 100 microlitres place on the foursquare sheet glass of 2cm * 2cm, smear evenly, dry naturally.On sheet glass, add water 100 microlitres, wiped liquid is coated with cotton balls and played the silkworm body surface 2 ages to moistening, culture dish lid built-in dip in water filter paper preserve moisture raise 24 hours after, change into normally and raising;
This experiment raising " Invest, Then Investigate " incidence of disease in two length of time.
B, Aspergillus test
The medication group:
With Aspergillus bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) 100 microlitres place on the foursquare sheet glass of 2cm * 2cm, smear evenly, dry naturally.After sheet glass being put into the cupboard Smudging sterilizing 60min of sealing, sheet glass taken out add water 100 microlitres, has wiped with cotton balls that liquid is coated with the newly-hatched silkworm body surface is extremely moistening, culture dish cover built-in dip in water filter paper preserve moisture raise 24 hours after, change into normally and raising;
Control group:
With Aspergillus bacterium liquid (bacterial concentration: 5.0 * 10
7Individual/ml) 100 microlitres place on the foursquare sheet glass of 2cm * 2cm, smear evenly, dry naturally.On sheet glass, add water 100 microlitres, wiped with cotton balls liquid is coated with the newly-hatched silkworm body surface to moistening, culture dish lid built-in dip in water filter paper preserve moisture raise 24 hours after, change into normally and raising;
This experiment raising " Invest, Then Investigate " incidence of disease in two length of time.
Experimental result is as shown in table 5:
Table 5
The above is merely preferred embodiment of the present invention, and is in order to restriction the present invention, not all within spirit of the present invention and principle, any modification of being done, is equal to replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (6)
1. a smoking disinfectant for silkworm is characterized in that, is made up of following raw material: chlorobromide isocyanuric acid 1～20 weight portion, combustion adjuvant 1～5 weight portion.
2. smoking disinfectant for silkworm according to claim 1 is characterized in that, said chlorobromide isocyanuric acid is 2～4 weight portions.
3. according to claim 1 or 2 arbitrary described smoking disinfectant for silkworm, it is characterized in that said combustion adjuvant is any one in flour, wood dust, the carbon dust.
4. smoking disinfectant for silkworm according to claim 3 is characterized in that, said combustion adjuvant is a flour.
5. a smoking disinfectant for silkworm as claimed in claim 3 is cultured the application in the diseases prevention at silkworm room disinfection of tools for silkworm rearing and/or silkworm.
6. the preparation method of a smoking disinfectant for silkworm is characterized in that, comprising: get the chlorobromide isocyanuric acid of 1～20 weight portion and the combustion adjuvant of 1～5 weight portion, packing promptly gets respectively.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012102720455A CN102812957A (en) | 2012-07-31 | 2012-07-31 | Smoking disinfectant for silkworms, preparation method and application thereof |
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| CN2012102720455A CN102812957A (en) | 2012-07-31 | 2012-07-31 | Smoking disinfectant for silkworms, preparation method and application thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103392708A (en) * | 2013-08-15 | 2013-11-20 | 山东省蚕业研究所 | Special disinfectant for artificial diet rearing of silkworms and application thereof |
| CN104604868A (en) * | 2015-01-04 | 2015-05-13 | 山东省蚕业研究所 | Disinfectant for silkworm rearing rooms, silkworm rearing instruments and silkworm rearing beds and application of disinfectant |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1123599A (en) * | 1995-08-10 | 1996-06-05 | 烟台蚕药试验厂 | Smoking disinfectant for silkworm and its preparation |
-
2012
- 2012-07-31 CN CN2012102720455A patent/CN102812957A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1123599A (en) * | 1995-08-10 | 1996-06-05 | 烟台蚕药试验厂 | Smoking disinfectant for silkworm and its preparation |
Non-Patent Citations (1)
| Title |
|---|
| 曹志贤等: "三种空气消毒方法消毒效果的比较", 《中国消毒学杂志》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103392708A (en) * | 2013-08-15 | 2013-11-20 | 山东省蚕业研究所 | Special disinfectant for artificial diet rearing of silkworms and application thereof |
| CN103392708B (en) * | 2013-08-15 | 2015-03-11 | 山东省蚕业研究所 | Special disinfectant for artificial diet rearing of silkworms and application thereof |
| CN104604868A (en) * | 2015-01-04 | 2015-05-13 | 山东省蚕业研究所 | Disinfectant for silkworm rearing rooms, silkworm rearing instruments and silkworm rearing beds and application of disinfectant |
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Application publication date: 20121212 |