CN102871885B - A kind of hair growth promoter and application thereof - Google Patents

Abstract

The invention discloses a hair tonic, which takes humic acid sodium salt and/or potassium salt as an essential component, and has the functions of promoting blood circulation, removing blood stasis, nourishing hair follicles, growing hair and moisturizing hair, and preventing hair loss; the humic acid described in the invention Sodium (potassium) salt can promote blood circulation, promote the hair growth of depilated mice, and have a certain inhibitory effect on the hair loss of mice injected with cyclophosphamide. By using sodium humate (potassium) salt as the main active ingredient, it provides a hair growth agent and a hair growth method that have good effects of promoting hair growth and preventing hair loss, are natural, have no irritating smell, have no toxic side effects, and are safe and reliable.

Description

A kind of hair tonic and its application

technical field

The invention relates to a hair tonic. The hair tonic uses sodium (potassium) humate as an essential component and has the functions of promoting hair growth and preventing hair loss. The hair tonic is used in hair growth, hair care, and hair care products (including medicines) , health products or cosmetics).

Background technique

Humic acid (HA) is a natural organic substance formed and accumulated through the decomposition and transformation of animal and plant remains through a series of geochemical processes. It can be divided into fulvic acid, brown humic acid and black humic acid according to its molecular weight. There are three types of acids, containing a variety of active groups, such as carboxyl, phenolic hydroxyl, alcoholic hydroxyl, quinone, carbonyl, etc., with a variety of biological activities. As early as in ancient my country, Li Shizhen's "Compendium of Materia Medica" had records about humic acid-like drugs. At present, a large number of experimental studies and clinical curative effects at home and abroad have shown that it has significant effects such as promoting blood circulation, removing blood stasis, anti-inflammatory, and antibacterial.

Hair loss is a frequently-occurring clinical disease and a common disease. When more than 100 hairs are lost every day, it can be judged as pathological alopecia, mainly including androgenetic alopecia, alopecia areata, pseudoalopecia areata and anagen alopecia. Hair loss has been an important problem that plagues people for a long time, affecting people's daily life. Its etiology is more complicated, especially with the increase of people's life and work pressure, the incidence rate is very high, which seriously affects the appearance. At present, there are some hair growth products in the market, but there are some common problems, such as: (1) Traditional Chinese medicine: There are many reports on the treatment of hair loss in traditional Chinese medicine, but there is no unified treatment; some Chinese medicine itself has irritation or adverse effects. Odor; active ingredients of traditional Chinese medicine are mostly extracted by organic solvents, and there are likely to be toxic and harmful solvent residues; some products are dissolved in organic solvents, even if the solvent is ethanol, there is a pungent smell, and some people may even have skin allergies; some traditional Chinese medicine hair growth products There are too many raw materials in the formula, the production process is relatively complicated, the product quality is difficult to control, and the cost is high; (2) Western medicine: most of them are hormones or irritating medicines, with side effects, even after discontinuation or long-term use , recurrence occurs.

Contents of the invention

According to the causes and characteristics of hair loss, and aiming at the shortcomings of existing products and technologies, the purpose of the present invention is to provide a hair growth agent with ideal hair growth and anti-hair loss effects, no toxic side effects, and no pungent smell. The hair tonic contains humic acid sodium salt and/or humic acid potassium salt.

In the present invention, the sodium humic acid salt is sodium fulvic acid and/or sodium brown humate, the potassium salt of humic acid is potassium fulvic acid and/or potassium brown humate, and two or more humic acid salts are mixed in any ratio.

The dosage of humic acid sodium salt and/or humic acid potassium salt in the present invention is 1g/L-30g/L.

In the present invention, humic acid is prepared and purified from peat and lignite by H ₂ 0 ₂ catalytic oxidation degradation method, and the ash content of humic acid after purification is ≤1%; while fulvic acid and brown humic acid are separated and refined from purified humic acid , obtained by removing black phytic acid; humic acid sodium salt or potassium salt is to add alkali reaction to the humic acid prepared by the above method to obtain humic acid sodium salt or potassium salt; for example, refer to the application number 200810233669.X "Lignite oxidation Degradation method for producing humic acid and its salt".

Physicochemical indicators of fulvic acid: total acidic group 13.82mmol/g, carboxyl group 10.18mmol/g, phenolic hydroxyl group 3.64mmol/g, components with molecular weight less than or equal to 1000 are 75%, C, H, N, O element content (%) respectively For 38.77, 4.166, 1.993, 55.07.

The hair tonic of the present invention is a hair tonic that contains one or more mixtures (in any ratio) of humic acid sodium salt and/or humic acid potassium salt as active ingredients for percutaneous administration from the head.

Another object of the present invention is to use the aforementioned hair growth agent containing one or more mixtures of humic acid sodium salt and/or humic acid potassium salt as active ingredients for the preparation of hair growth, hair care, and hair care products (including medicines, health care products, etc.) products or cosmetics), its dosage form can be lotion, emulsion, emulsion (ointment), gel and spray, etc., and it can be manufactured according to the purpose of dosage form using conventional methods.

When hair growth agents are used to prepare hair growth, hair care, and hair care products, conventional auxiliary mixing ingredients used in the preparation of conventional cosmetics, health products, and pharmaceuticals can be added as needed, such as excipients and vasodilation commonly used in hair growth agents. Agents, moisturizing agents, various animal and plant active extracts, vitamins, antioxidants, water, cosolvents, metabolism revitalizing agents, surfactants, spices and dyes, etc., can be used in combination within the scope of not affecting the effect of the present invention .

When the hair restorer of the present invention is used, it directly acts on the hair-growing part, promotes and induces hair growth and development through the skin, and makes the hair thicker and thicker.

Compared with prior art, advantage and technical effect of the present invention are as follows:

1. The hair growth agent provided by the present invention with humic acid sodium salt and/or potassium salt as the main component can activate blood circulation, remove blood stasis, nourish hair follicles, and has a good effect on promoting hair growth and preventing hair loss.

_2. Humic acid as the main active ingredient of the present invention is prepared and purified from peat and lignite by H2O2 catalytic oxidation degradation method, while fulvic _acid and brown humic acid are separated and refined from purified humic acid, removed obtained from black phytic acid. Physicochemical indicators of fulvic acid: total acidic group 13.82mmol/g, carboxyl group 10.18mmol/g, phenolic hydroxyl group 3.64mmol/g, components with molecular weight less than or equal to 1000 are 75%, C, H, N, O element content (%) respectively For 38.77, 4.166, 1.993, 55.07.

3. Strictly define the sources of humic acid as peat and lignite, which can avoid some toxic and side effects in the later stage. Humic acid is prepared by H ₂ O ₂ catalytic oxidation degradation method. The process is simple, green and environmentally friendly, and the product has high biological activity. The sodium or potassium salt of humic acid obtained is natural, has no irritation or bad smell, and does not have toxic and harmful solvent residues. High, non-toxic and side effects, safe and reliable.

4. The present invention has a wide range of applications, and has good curative effects on seborrheic alopecia, alopecia areata, mixed alopecia, and alopecia during cancer treatment.

Description of drawings

Fig. 1 is a schematic diagram of the scoring curve of new hair growth status of mice in the present invention.

Fig. 2 is a schematic diagram showing the comparison of skin blood vessel distribution in the hair removal area of mice according to the present invention, A is the blank group; B is the drug-administered group.

3 is a schematic diagram of a blank group of mouse back skin HE stained sections of the present invention;

Fig. 4 is a schematic diagram of the dose group in the HE stained section of mouse back skin of the present invention;

Fig. 5 is a picture of mice containing fulvic acid sodium hair growth agent administered for 0 days in the present invention;

Fig. 6 is a picture of a mouse administered with sodium fulvic acid-containing hair growth agent for 8 days in the present invention;

Fig. 7 is a picture of a mouse administered for 16 days with the hair growth agent containing sodium fulvic acid of the present invention.

Detailed ways

The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments, but the protection scope of the present invention is not limited to the content described.

Example 1: Experiment of the Effect of Sodium Fulvic Humate on Hair Growth of Depilatory Mice

The present embodiment adopts normal mice to test the effect of sodium fulvic acid humate of the present invention on the hair growth of depilatory mice, and conducts the pharmacodynamic effect of continuously applying sodium fulvic acid aqueous solution for 15 days to the depilatory area of depilatory mice. observed.

The experimental materials used in this embodiment are as follows:

(1) Sodium fulvic acid: Sodium fulvic acid was prepared and purified by lignite H ₂ O ₂ catalyzed oxidative degradation method. The method refers to the method of application number 200810233669.X "Method for producing humic acid and its salt by oxidative degradation of lignite". method.

(2) The experimental animals were clean-type Kunming white mice, male, weighing 18-22 g, purchased from the Experimental Animal Center of Kunming Medical College, observed for two days before the experiment, and selected lively and healthy mice for the experiment.

The specific operation is as follows: take a Kunming mouse, under light anesthesia, heat and melt the mixture of rosin and paraffin wax with a mass ratio of 1:1, and then apply it on the back of the mouse, and gently remove it after it solidifies and hardens, The depilated area of the mice was about 2×2cm ² , and they were randomly divided into 4 groups according to body weight after depilation, with 10 mice in each group, a total of 4 groups, as follows:

(1) Blank control group: apply water to the hair removal area;

(2) Sodium fulvic acid low-dose administration group: smear 1g/L sodium fulvic acid aqueous solution;

(3) Medium dose administration group of sodium fulvic acid: smear 5g/L sodium fulvic acid aqueous solution;

(4) sodium fulvic acid high dose administration group: smear 25g/L sodium fulvic acid aqueous solution;

The drug was administered once a day for 15 consecutive days, and was sacrificed on the 16th day. The hair length and hair weight of the new hair area were measured, and the back skin was taken to observe the distribution of blood vessels. Observe the hair growth of animals in each group every day, and score the growth of new hair in the depilated area of mice every day. The scoring criteria are: ① no hair growth is 0 points; ② light hair and full growth are 1 point; ③ new hair length And the density is half of that of the undepilated area, 2 points; ④ There is no difference between the new hair growth and the undepilated area, 3 points; the scores are curve-fitted, and the results are shown in Table 1, Figure 1 and Figure 2.

The results show that sodium fulvic acid has the effect of promoting hair growth in depilatory mice, and there is a significant difference between the administration group and the blank group, and the skin blood vessels in the administration area of the administration mice are obviously richer and thicker.

Table 1: Effect of Sodium Fulvic Humate on Hair Growth in Depilatory Mice ( ±s)

In the table: compared with the blank control group, * means P<0.05, which has statistical significance, and ** means P<0.01, which has significant difference.

Example 2: Histological observation experiment on the effect of potassium brown humate on the hair growth of depilatory mice

This experimental example adopts normal mice to test the effect of potassium brown humate of the present invention on the hair growth of depilatory mice, and conducts a pharmacodynamic study on the continuous smearing of potassium brown humate water agent for 15 days in the depilatory area of depilatory mice. Observed, and made paraffin section of the skin in the new hair area, stained with HE, and carried out histological observation.

The experimental materials used in this embodiment are as follows:

(1) Potassium brown humate: Potassium brown humate was prepared and purified by conventional H ₂ O ₂ oxidative degradation lignite method. The method refers to the method in the application number 200810233669.X "Method for producing humic acid and its salt by oxidative degradation of lignite". method.

(2) The experimental animals were clean Kunming mice, male, weighing 18-22g, purchased from the Experimental Animal Center of Kunming Medical College, observed for two days before the experiment, and selected active and healthy mice for the experiment.

The specific operation of the experiment is as follows: take a Kunming mouse, under light anesthesia, heat and melt the rosin/paraffin mixture with a mass ratio of 1:1, and then apply it on the back of the mouse. The depilated area of each mouse was about 2×2cm ² , and they were randomly divided into groups according to body weight after depilation, with 10 mice in each group, 4 groups in total, as follows:

(1) Blank control group: apply water to the hair removal area;

(2) Potassium brown humate low-dose administration group: smear 1g/L of potassium brown humate aqueous solution;

(3) Potassium brown humate medium-dose administration group: smear 5g/L potassium brown humate aqueous solution;

(4) Potassium brown humate high-dose administration group: smear 30g/L potassium brown humate aqueous solution;

Administered once a day for 15 consecutive days, and sacrificed on the 16th day, made paraffin sections of the skin in the new hair area, HE stained, carried out histological observation, and counted hair follicles, the number of hair follicles in 3 high power fields (×400) per case, The average value was taken, and then the average number of hair follicles per mouse in each group was calculated, and the significance of the differences among the groups was analyzed. The results are shown in Table 2, Figure 3 and Figure 4.

The results of the experiment showed that after general histological observation, the skin was found to be intact, without reactions such as hyperemia, edema, and inflammatory cell infiltration, indicating that the animal skin tissue had no damage, irritation, and inflammatory response; potassium brown humate has the effect of promoting hair growth in mice, The number of hair follicles increased and the thickness of the dermis thickened significantly, compared with the blank group, there was a very significant difference.

Table 2: Potassium brown humate on hair growth histological observation experiment of depilatory mice ( ±s)

In the table: compared with the blank control group, * means P<0.05, which has statistical significance, and ** means P<0.01, which has significant difference.

Example 3: The mixture of sodium fulvic humate and sodium brown humate (1:5) inhibits hair loss in mice

This experimental example adopts the mouse of intraperitoneal injection of cyclophosphamide to test the effect of sodium humate of the present invention on inhibiting hair loss in mice, and continuously smears sodium fulvic acid and sodium humate on the back area of mice injected with cyclophosphamide The pharmacodynamics of brown humic acid sodium mixture (1:5) water agent was observed for 28 days, and it was found that the drug had inhibitory effect on the hair loss of mice injected with cyclophosphamide intraperitoneally, and the blood vessels in the skin of the treated mice were obvious Become rich and increase in gross weight.

The experimental materials used in this embodiment are as follows:

(1) Sodium fulvic humate and sodium brown humate: prepared and purified by conventional H ₂ O ₂ oxidative degradation of lignite, the method refers to the application number 200810233669.X "Method for producing humic acid and its salt by oxidative degradation of lignite" Methods.

(2) Cyclophosphamide: Jiangsu Hengrui Pharmaceutical Co., Ltd., prepared at a concentration of 10 mg/mL, with a dosage of 100 mg/kg mouse body weight.

(3) The experimental animals were clean-type Kunming mice, male, weighing 18-22 g, purchased from the Experimental Animal Center of Kunming Medical College, observed for two days before the experiment, and selected lively and healthy mice for the experiment.

The specific operation is as follows: Kunming mice were taken, and after intraperitoneal injection of cyclophosphamide, they were randomly divided into groups according to body weight, with 8 mice in each group, totally 4 groups, as follows:

(1) Blank control group: apply water;

(2) Low-dose administration group of sodium fulvic humate and sodium brown humate mixture (1:5): apply 1g/L liquid medicine;

(3) Medium-dose administration group of sodium fulvic humate and sodium brown humate mixture (1:5): apply 5g/L liquid medicine;

(4) High-dose administration group of sodium fulvic humate and sodium brown humate mixture (1:5): apply 25g/L liquid medicine;

The drug was administered once a day for 28 consecutive days, and was executed on the 29th day. The gross weight of the rats in the area where the drug was applied was measured, and the skin on the back was taken to observe the distribution of blood vessels, as shown in Table 3.

The results showed that the mixture of sodium fulvic humate and sodium brown humate (1:5) could promote hair growth in mice, and inhibit hair loss in mice injected with cyclophosphamide intraperitoneally, and the blood vessels in the skin of the treated mice were obvious Become rich and increase in gross weight.

Table 3: The effect of the mixture of sodium fulvic acid and sodium brown humate on hair loss in mice injected with cyclophosphamide ( ±s)

Embodiment 4: Effect experiment of hair growth agent containing sodium fulvic acid on hair growth of depilatory mice

The composition and weight distribution ratio of the hair tonic containing sodium fulvic acid in this embodiment are as follows (the solvent is water):

Sodium Fulvic Humate 5g/L Angelica 20g Polygonum Polygoni Multiflori 15g

Arborvitae leaf 15g Wild chrysanthemum 10g

Preparation method: Mix the above-mentioned medicines and pulverize them, soak them for 24 hours according to the medicine-water ratio of 1:4, boil them, cool them and filter them. Add sodium fulvic acid into the liquid medicine.

The water-based hair tonic is natural, has no irritation or bad smell, no toxic and harmful solvent residues, no toxic and side effects, and is safe and reliable. The verification method adopted is the same as that in Example 1. Zhangguang 101 is used as the positive control, and no sodium fulvic acid hair growth agent is used as the negative control. The results are shown in Table 4, Figures 5-7.

The results showed that the hair growth agent containing sodium fulvic acid was better absorbed by the skin of the mice, the hair was smooth, and the skin blood vessels in the medication area became significantly richer, which had the effect of promoting the hair growth of the depilatory mice. The hair growth of the administration group and the blank group , Gross weight have significant difference, the effect is slightly better than the positive control.

Table 4: Effects of hair growth agents containing sodium fulvic acid on hair growth in depilatory mice ( ±s)

group Hair length (cm) Gross weight g/(cm ² ) blank control 0.61±0.08660 0.015±0.0040 Positive control (Zhangguang 101) 0.662±0.05178 0.0192±0.0033 Sodium Fulvic Humate Hair Tonic 0.764±0.05029* 0.02098±0.0023* negative control 0.667±0.05111 0.0178±0.0042

In the table: compared with the blank control group, * means P<0.05, which has statistical significance, and ** means P<0.01, which has significant difference.

Example 5: Hair growth agent containing a mixture of sodium fulvic acid and potassium fulvic acid (1:3)

The composition and weight distribution ratio of hair tonic are as follows (solvent is water):

Mixture of Sodium Fulvic Humate and Potassium Fulvic Humate (1:3) 5g/L Ginger 20g

Pinellia 15g Arborvitae leaf 15g Morus alba bark 10g

Preparation method: Decoct ginger, Pinellia, Arborvitae leaves, and Morus alba in water twice, cool and filter; add the mixture of sodium fulvic acid and potassium fulvic acid (1:3) into the liquid medicine, and the product is a water preparation.

Claims (2)

1. A hair restorer, characterized in that: containing humic acid sodium salt and/or humic acid potassium salt; Wherein the dosage of said humic acid sodium salt and/or humic acid potassium salt is 1g/L-30g/L; The humic acid sodium salt and humic acid potassium salt are prepared as follows: using peat and lignite as raw materials, using H ₂ 0 ₂ catalytic oxidation degradation method to prepare and purify humic acid, the humic acid ash content after purification is ≤ 1%; Separating and refining fulvic acid and brown humic acid from the purified humic acid; adding alkali to the fulvic acid and brown humic acid to obtain humic acid sodium or potassium salt; Wherein said humic acid sodium salt is sodium fulvic acid and/or sodium brown humic acid; The potassium humate is potassium fulvic acid and/or potassium brown humate. 2. the application of the hair tonic described in claim 1 in the preparation of hair growth, hair care, and hair care products.