CN104604689B - 快速获得菊苣外植体和提高其出愈率的方法 - Google Patents
快速获得菊苣外植体和提高其出愈率的方法 Download PDFInfo
- Publication number
- CN104604689B CN104604689B CN201510063985.7A CN201510063985A CN104604689B CN 104604689 B CN104604689 B CN 104604689B CN 201510063985 A CN201510063985 A CN 201510063985A CN 104604689 B CN104604689 B CN 104604689B
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- Prior art keywords
- explant
- witloof
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- acquisition
- seed
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- 235000007542 Cichorium intybus Nutrition 0.000 title claims abstract description 23
- 244000298479 Cichorium intybus Species 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title claims abstract description 14
- 230000035876 healing Effects 0.000 title claims abstract description 11
- 206010020649 Hyperkeratosis Diseases 0.000 claims abstract description 10
- 230000001954 sterilising effect Effects 0.000 claims abstract description 10
- 230000006698 induction Effects 0.000 claims abstract description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 4
- 239000002609 medium Substances 0.000 claims description 18
- 235000015097 nutrients Nutrition 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 241000196324 Embryophyta Species 0.000 claims description 6
- 238000005286 illumination Methods 0.000 claims description 6
- 239000012528 membrane Substances 0.000 claims description 6
- 230000000050 nutritive effect Effects 0.000 claims description 6
- 230000008929 regeneration Effects 0.000 claims description 6
- 238000011069 regeneration method Methods 0.000 claims description 6
- 229920001817 Agar Polymers 0.000 claims description 3
- 241000723343 Cichorium Species 0.000 claims description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 3
- 229930006000 Sucrose Natural products 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- 230000000249 desinfective effect Effects 0.000 claims description 3
- 230000003203 everyday effect Effects 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 239000006870 ms-medium Substances 0.000 claims description 3
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims description 3
- 239000002689 soil Substances 0.000 claims description 3
- 239000005720 sucrose Substances 0.000 claims description 3
- 229910052902 vermiculite Inorganic materials 0.000 claims description 3
- 235000019354 vermiculite Nutrition 0.000 claims description 3
- 239000010455 vermiculite Substances 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 238000002255 vaccination Methods 0.000 abstract description 4
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Description
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510063985.7A CN104604689B (zh) | 2015-02-09 | 2015-02-09 | 快速获得菊苣外植体和提高其出愈率的方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510063985.7A CN104604689B (zh) | 2015-02-09 | 2015-02-09 | 快速获得菊苣外植体和提高其出愈率的方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104604689A CN104604689A (zh) | 2015-05-13 |
| CN104604689B true CN104604689B (zh) | 2016-01-06 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510063985.7A Expired - Fee Related CN104604689B (zh) | 2015-02-09 | 2015-02-09 | 快速获得菊苣外植体和提高其出愈率的方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104604689B (zh) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105112442B (zh) * | 2015-05-24 | 2019-01-11 | 贵州省草业研究所 | 免预培养高效菊苣遗传转化方法 |
| CN105104183B (zh) * | 2015-08-26 | 2017-07-04 | 北京农业生物技术研究中心 | 一种菊苣原生质体诱导纯合四倍体植物的方法 |
| CN115885852A (zh) * | 2022-12-21 | 2023-04-04 | 吉林农业科技学院 | 一种结球红菊苣叶片组培方法 |
| CN117121813A (zh) * | 2023-06-30 | 2023-11-28 | 江苏省中国科学院植物研究所 | 一种菊苣的高效再生方法 |
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2015
- 2015-02-09 CN CN201510063985.7A patent/CN104604689B/zh not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN104604689A (zh) | 2015-05-13 |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Method for rapidly obtaining chicory explant and improving its healing rate Effective date of registration: 20211216 Granted publication date: 20160106 Pledgee: Yantai financing guarantee Group Co.,Ltd. Pledgor: LUDONG University Registration number: Y2021980015152 |
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| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20220317 Granted publication date: 20160106 Pledgee: Yantai financing guarantee Group Co.,Ltd. Pledgor: LUDONG University Registration number: Y2021980015152 |
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| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
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Effective date of registration: 20220613 Address after: 315181 No. 573, 575, 577, 579 (1-8) -2, Jinjia 1st Road, Yinzhou District, Ningbo City, Zhejiang Province Patentee after: Ningbo Heyang Biotechnology Co.,Ltd. Address before: 264025 No. 186 Hongqi Middle Road, Zhifu District, Shandong, Yantai Patentee before: LUDONG University |
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| CI03 | Correction of invention patent | ||
| CI03 | Correction of invention patent |
Correction item: Patentee|Address Correct: Ludong University|264025 No. 186 Hongqi Middle Road, Zhifu District, Shandong, Yantai False: Ningbo Heyang Biotechnology Co.,Ltd.|315181 No. 573, 575, 577, 579 (1-8) -2, Jinjia 1st Road, Yinzhou District, Ningbo City, Zhejiang Province Number: 25-02 Volume: 38 |
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| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220704 Address after: 315181 No. 573, 575, 577, 579 (1-8) -2, Jinjia 1st Road, Yinzhou District, Ningbo City, Zhejiang Province Patentee after: Ningbo Heyang Biotechnology Co.,Ltd. Address before: 264025 No. 186 Hongqi Middle Road, Zhifu District, Shandong, Yantai Patentee before: LUDONG University |
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
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Granted publication date: 20160106 |