CN104396960A

CN104396960A - Pesticidal composition

Info

Publication number: CN104396960A
Application number: CN201410579095.7A
Authority: CN
Inventors: 杨会营; 刘晓娇; 夏小东; 吴勇; 马嵩岳
Original assignee: Beijing Yanhua Yongle Biotechnology Co Ltd
Current assignee: Beijing Yanhua Yongle Biotechnology Co Ltd
Priority date: 2014-10-25
Filing date: 2014-10-25
Publication date: 2015-03-11

Abstract

Belonging to the technical field of pesticides, the invention relates to a pesticidal composition, which is characterized by including chlorobenzene ether amide and azoxystrobin as the active ingredients. The composition can be prepared into suspending agents and water dispersible granules, has obvious synergistic effect and prolonged duration, and is friendly to the environment.

Description

Composition pesticide

Technical field

The invention belongs to technical field of pesticide, contain the bactericidal composition of chlorobenzene ether acid amides and Fluoxastrobin specifically.

Background technology

Chlorobenzene ether acid amides is Central China Normal University's pyrazol acid amide compounds newly developed, and epidemic disease, the downy mildew common to cucumber, tomato, potato, fruit tree leaves pinta has extraordinary effect, and active ingredient is novel, non-resistant.

Fluoxastrobin (Azoxystrobin) belongs to methoxy acrylic bactericide; it is mitochondrial respiratory inhibitor; bactericidal activity is wide; to germ, there is protection, treatment, eradicant action; almost there is good activity to all fungi ridge (Ascomycetes, list Gammaproteobacteria, Oomycete and Fungi Imperfecti) disease such as tens of kinds of diseases such as powdery mildew, rust, glume blight, scab, downy mildew, rice blast; meanwhile, seed treatment and soil treatment can also be applied to.

Cucumber downy mildew, also known as title " horse race is dry ", " cured leaf ", is that green house cucumber occurs one of the most general, harm is the most serious disease.This sick morbidity scope is wide, and all there is generation in the area of every plantation cucumber; And all can occur the time of infertility cucumber, when developmental condition is suitable for, quickly, 3 ~ 5d can make whole plant leaf (except several pieces tender leafs of top and indivedual Lao Ye) withered and yellow to its development speed.The general popular time can the underproduction 20% ~ 30%, and time serious, loss reaches more than 50% even whole canopy room and gets nowhere, and has a strong impact on vegetable grower's income.

Summary of the invention

The object of this invention is to provide and a kind ofly there is synergistic effect and the bactericidal composition of safety and environmental protection, realize improving drug effect, decreasing pollution, the object that reduces costs.Be specifically related to the bactericidal composition of chlorobenzene ether acid amides and azoxystrobin compound.

Utilize the bactericidal composition described in the invention described above, be equipped with the known auxiliary agent of those skilled in that art, make formulation of the present invention with the known method of those skilled in that art, comprise suspending agent and water dispersible granules.

In above-mentioned composition, the proportion of chlorobenzene ether acid amides and Fluoxastrobin is 1:50-50:1.

Above-mentioned composition has following characteristics: 1. formulation environmental protection, and environmental pollution is little; 2. composition is novel, and bactericidal action is strong, not easily develops immunity to drugs; 3. two kinds of compositions are composite, have synergistic effect; 4. between two-component without cross resistance.

Embodiment

For determining chlorobenzene ether acid amides and the composite synergistic effect of Fluoxastrobin two kinds of active ingredients, first applicant uses these two kinds of compositions to carry out indoor biometrics test, test method and result as follows:

First after analyzing the action character of two kinds of active ingredients, the raw object surveyed is decided to be Cuba artificial downy mildew (Pseudoperonospora cubensis) by us, this Pseudomonas Mastigomycotina fungi, germ is live body obligatory parasitism fungi, seed does not carry disease germs, germ is mainly propagated by air-flow, invades from Stoma of Leaves.The rate of propagation of this disease is very fast, and someone is referred to as " horse race is dry ", once there be primary infection focus, only need the expansion of 3 ~ 4 times to infect, can lead to terrible disaster, the key of therefore preventing and treating this disease finds as early as possible, early control again.

The test method of indoor biometrics is with reference to NYT 1156.6-2006: adopt the inhibitory action that growth rate method mensuration medicament grows cucumber downy mildew mycelia.On the basis of preliminary experiment, from each medicament, design 5 concentration within the scope of 10%-90% are reached to germ mycelial growth inhibition rate, it is for subsequent use by the 95% former medicine of chlorobenzene ether acid amides and the former medicine of 95% Fluoxastrobin with methyl alcohol to be first that solvent is made into the liquid of series concentration, then liquid is joined in 1% ratio in the PDA medium melting also cool to room temperature, after fully shaking up, pour in the culture dish (Φ 90mm) of sterilizing and make band medicine flat board, often process and repeat, to add the process of sterile water for blank for four times; With card punch (Φ 5mm) the picking bacterium of downy mildew of cucumber bacterium cake of sterilizing, mycelia faces down aseptic inoculation in the central authorities of band medicine flat board, be inverted in the interior cultivation of constant incubator of 25 DEG C, adopt right-angled intersection method to measure colony diameter after cultivating 6d, calculate the EC of each medicament to pathogen ₅₀, and calculate toxicity index according to the following equation, compare virulence and the bacteriostasis of different agents.Above-mentioned raw survey experiment former medicine used and reagent are all bought from other producers.

The co-toxicity coefficient method representation that the co-toxicity of mixture preparation adopts Sun Yun abundant:

The theoretical toxicity index TTI=∑ of mixture preparation (percentage of this pharmaceutically active ingredient of the toxicity index ATI of certain medicine × in mixture).

Be synergistic effect when co-toxicity coefficient CTC is greater than 120, being antagonism when being less than 80, is addition time between 80-120.

Table 1 chlorobenzene ether acid amides, Fluoxastrobin and different ratio thereof are to the measurement result of cucumber downy mildew co-toxicity coefficient

Analyze above-mentioned data can obviously find out, when chlorobenzene ether acid amides and Fluoxastrobin are composite in varing proportions, the co-toxicity coefficient (CTC) of formula is all greater than 120, the prevention and control capability of cucumber downy mildew is had to the synergistic effect of different proportion.Visible, the complex preparation of chlorobenzene ether acid amides and Fluoxastrobin can become the favourable agents of control cucumber downy mildew.

Tested by indoor biometrics, determine chlorobenzene ether acid amides and Fluoxastrobin two kinds of active ingredients composite after, have good synergistic effect, below by way of instantiation in order to further describe the present invention, but the present invention is absolutely not only only limitted to these embodiments following.In formula, percentage is all weight percentage, and the former medicine used and auxiliary agent are other producers and buy.

Formulation examples is as follows:

Example one, 10.2% chlorobenzene ether acid amides Fluoxastrobin suspending agent

Example two, 3.1% chlorobenzene ether acid amides azoxystrobin water dispersible granule

Example three, 11% chlorobenzene ether acid amides Fluoxastrobin suspending agent

Example four, 6% chlorobenzene ether acid amides azoxystrobin water dispersible granule

Example five, 10% chlorobenzene ether acid amides Fluoxastrobin suspending agent

Example six, 12% chlorobenzene ether acid amides azoxystrobin water dispersible granule

Example seven, 3.3% chlorobenzene ether acid amides Fluoxastrobin suspending agent

Example eight, 31% chlorobenzene ether acid amides azoxystrobin water dispersible granule

Example nine, 15.3% chlorobenzene ether acid amides Fluoxastrobin suspending agent

Select above example to carry out preventing and treating the field trial of cucumber downy mildew, determine to fill a prescription and prevent and treat the effect of cucumber downy mildew; Simultaneously by the Contrast on effect with chlorobenzene ether acid amides and Fluoxastrobin single dose, verify composite after synergistic effect.Concrete test method data are as follows:

6% chlorobenzene ether acid amides azoxystrobin water dispersible granule and other preparation do the test of pesticide effectiveness of field control cucumber downy mildew, and test is with reference to GB/T17980.26-2000.Test site is Yangling Shaanxi, and the time is on October 13rd, 2013, executes first pass medicine, and October 20 executed second time medicine.Test each process three repetition, community randomized arrangement, each plot area is 20m ², adopt back-pack electric compression sprayer, dispenser was sunny calm for the same day, and the planting habit of planting habit all with local of all communities is identical, records the synoptic data on the dispenser same day and a week thereafter, specific as follows:

Table 2 duration of test meteorological data

Investigation method:

Each community random searching 4 points, each some investigation 2 strain cucumber, record investigation total number of sheets, blade disease index.

Investigation number of times:

Carry out the investigation of state of an illness radix on October 13rd, 2013 before first time dispenser, first time medicine 7 days day after tomorrow (October 20) carries out second time investigation and second time dispenser, within 6 days, carries out third time investigation, investigate 3 times altogether after second time medicine.

The grade scale of disease index:

0 grade: without scab;

1 grade: lesion area accounts for less than 5% of whole leaf area;

3 grades: lesion area accounts for 6% ~ 10% of whole leaf area;

5 grades: lesion area accounts for 11% ~ 25% of whole leaf area;

7 grades: lesion area accounts for 26% ~ 50% of whole leaf area;

9 grades: lesion area accounts for more than 51% of whole leaf area.

Table 3 6% chlorobenzene ether acid amides azoxystrobin water dispersible granule and different agents are to the preventive effect of cucumber downy mildew

As can be seen from above-mentioned data, chlorobenzene ether acid amides and azoxystrobin compound preparation after medicine 7 days preventive effects all more than 76%, and chlorobenzene ether acid amides single dose 7 days preventive effects after first time medicine are 48.45%, the preventive effect of Fluoxastrobin single dose is 48.66%, compare chlorobenzene ether acid amides and Fluoxastrobin single dose, complex preparation preventive effect is more remarkable.Visible, there is good synergistic effect after two kinds of active ingredients are composite.And after second time medicine 6 days, all complex preparations have all exceeded 87% to the preventive effect of cucumber downy mildew, and the lasting period of complex preparation extends, more lasting to the control efficiency of cucumber downy mildew, alleviate the financial resources of peasant, material resources, have very high practicality.

Claims

1. a composition pesticide, is characterized in that: active component is made up of chlorobenzene ether acid amides and Fluoxastrobin, and the weight ratio of described chlorobenzene ether acid amides and Fluoxastrobin is 1:50 ~ 50:1.

2. composition according to claim 1, is characterized in that: make suspending agent, water dispersible granules by active component and auxiliary material.