CN104396943A - Pangasias sutchi semen storage method - Google Patents
Pangasias sutchi semen storage method Download PDFInfo
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- CN104396943A CN104396943A CN201410714797.1A CN201410714797A CN104396943A CN 104396943 A CN104396943 A CN 104396943A CN 201410714797 A CN201410714797 A CN 201410714797A CN 104396943 A CN104396943 A CN 104396943A
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Abstract
A pangasias sutchi semen storage method comprises the following steps of semen collection, diluent preparation, semen dilution and semen freezing. The method improves a semen utilization ratio and an outbreeding fertilization rate of pangasias sutchi, has low condition requirements, and is easy to implement, and suitable for large-scale culture and breeding.
Description
Technical field
The present invention relates to fish reproduction technology, belong to technical field of aquaculture, be specifically related to fresh water white shark Semen routine method.
Background technology
The white shark of fresh water, is the famous and precious fish of fresh water, is mainly distributed in Southeast Asia one band, originates in the ground such as Malaysia, Thailand, introduces China in 1998.This fish divides black, white, ash three kinds of body colours, and body is long flat-sided, and body surface smooth is without squama, and back is swelled, conically flat, and mouth is sub-the next, and gill film is not connected with cheek.Wooden dipper is large, has the function of breathe air, not easily anoxic.Fresh water white shark body colour is gorgeous, has that growth is fast, feeding habits assorted, an advantage such as thorn, fine and tender taste between lower oxygen concentration resistance, premunition strong, few flesh, and thus market prospects are incomparably wide.The cryopreservation method of fish sperm is mature on the whole, but species variation obviously exists, and does not have the report of this respect in the Semen routine field relating to the white shark of fresh water.
Summary of the invention
The technical problem that the present invention mainly solves is to provide a kind of fresh water white shark Semen routine method.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is: provide a kind of fresh water white shark Semen routine method, comprise the following steps:
(1) semen collection: gather the good white shark seminal fluid of fresh water of maturity, for subsequent use under depositing in 3 DEG C of clean environment;
(2) diluent preparing: preparation is containing the solution of weight ratio streptomycin 0.09%, NaCl 0.65%, KCl 0.21%, glucose 1.5%, sucrose 0.5%, sodium glutamate 0.8%, and adjust ph is 6.8;
(3) sperm dilution: by above-mentioned seminal fluid and dilution with after 1: 3 dilution ratio mixed diluting, adding antifreeze to final concentration is 8%, and balances 35 minutes under being put in 3 DEG C of conditions;
(4) semen freezing: the seminal fluid after above-mentioned dilution balance is placed in liquid nitrogen ullage 5 centimeters and balances 15 minutes, then above liquid nitrogen surface 1 centimeters to drop into Liquid nitrogen storage after balancing 5 minutes for subsequent use.
Carry out quality examination after described semen collection step: visually observing normal semen is milky, with simple microscope, under 10 × 40 times, with clear water activate, average motility rate is greater than 80% Semen routine for subsequent use, otherwise, abandon it.
Described antifreeze is the composition of volume ratio DMSO10%, glycerine 30%, ethylene glycol 45%, DMA15%.
The invention has the beneficial effects as follows:
(1) improve fresh water white shark mirror availability and outbreeding fertilization rate;
(2) conditional request of the present invention is low, and operation is easy to realize, and is suitable for large-scale cultivation and breeds.
Embodiment
Below preferred embodiment of the present invention is described in detail, can be easier to make advantages and features of the invention be readily appreciated by one skilled in the art, thus more explicit defining is made to protection scope of the present invention.
The embodiment of the present invention comprises:
Embodiment
A kind of fresh water white shark Semen routine method, comprises the following steps:
(1) semen collection: gather the good white shark seminal fluid of fresh water of maturity, for subsequent use under depositing in 3 DEG C of clean environment;
(2) diluent preparing: preparation is containing the solution of weight ratio streptomycin 0.09%, NaCl 0.65%, KCl 0.21%, glucose 1.5%, sucrose 0.5%, sodium glutamate 0.8%, and adjust ph is 6.8;
(3) sperm dilution: by above-mentioned seminal fluid and dilution with after 1: 3 dilution ratio mixed diluting, adding antifreeze to final concentration is 8%, and balances 35 minutes under being put in 3 DEG C of conditions;
(4) semen freezing: the seminal fluid after above-mentioned dilution balance is placed in liquid nitrogen ullage 5 centimeters and balances 15 minutes, then above liquid nitrogen surface 1 centimeters to drop into Liquid nitrogen storage after balancing 5 minutes for subsequent use.
Carry out quality examination after described semen collection step: visually observing normal semen is milky, with simple microscope, under 10 × 40 times, with clear water activate, average motility rate is greater than 80% Semen routine for subsequent use, otherwise, abandon it.
Described antifreeze is the composition of volume ratio DMSO10%, glycerine 30%, ethylene glycol 45%, DMA15%.
The white shark seminal fluid fertilization of the fresh water utilizing the present invention to preserve, can reach 50%, higher than existing market about 20% at primitive gut fertilization rate in mid-term.
The foregoing is only embodiments of the invention; not thereby the scope of the claims of the present invention is limited; every utilize description of the present invention to do equivalent structure or equivalent flow process conversion; or be directly or indirectly used in other relevant technical fields, be all in like manner included in scope of patent protection of the present invention.
Claims (3)
1. a fresh water white shark Semen routine method, is characterized in that, comprise the following steps:
(1) semen collection: gather the good white shark seminal fluid of fresh water of maturity, for subsequent use under depositing in 3 DEG C of clean environment;
(2) diluent preparing: preparation is containing the solution of weight ratio streptomycin 0.09%, NaCl 0.65%, KCl 0.21%, glucose 1.5%, sucrose 0.5%, sodium glutamate 0.8%, and adjust ph is 6.8;
(3) sperm dilution: by above-mentioned seminal fluid and dilution with after 1: 3 dilution ratio mixed diluting, adding antifreeze to final concentration is 8%, and balances 35 minutes under being put in 3 DEG C of conditions;
(4) semen freezing: the seminal fluid after above-mentioned dilution balance is placed in liquid nitrogen ullage 5 centimeters and balances 15 minutes, then above liquid nitrogen surface 1 centimeters to drop into Liquid nitrogen storage after balancing 5 minutes for subsequent use.
2. fresh water according to claim 1 white shark Semen routine method, is characterized in that:
Carry out quality examination after described semen collection step: visually observing normal semen is milky, with simple microscope, under 10 × 40 times, with clear water activate, average motility rate is greater than 80% Semen routine for subsequent use, otherwise, abandon it.
3. fresh water according to claim 1 and 2 white shark Semen routine method, is characterized in that: described antifreeze is the composition of volume ratio DMSO10%, glycerine 30%, ethylene glycol 45%, DMA15%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410714797.1A CN104396943A (en) | 2014-11-28 | 2014-11-28 | Pangasias sutchi semen storage method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410714797.1A CN104396943A (en) | 2014-11-28 | 2014-11-28 | Pangasias sutchi semen storage method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104396943A true CN104396943A (en) | 2015-03-11 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410714797.1A Pending CN104396943A (en) | 2014-11-28 | 2014-11-28 | Pangasias sutchi semen storage method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104814005A (en) * | 2015-03-26 | 2015-08-05 | 谢光玉 | Preservation method for seminal fluid of Schizothorax davidi |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001028326A1 (en) * | 1999-10-20 | 2001-04-26 | Istituto Sperimentale Italiano 'lazzaro Spallanzani' | Method for cryopreservation of teleostei semen |
| CN1596670A (en) * | 2004-07-27 | 2005-03-23 | 中国水产科学研究院黄海水产研究所 | Practicalization method for frozen preserving sperm of fish |
| CN101088511A (en) * | 2007-02-15 | 2007-12-19 | 中国水产科学研究院长江水产研究所 | Simple method for ultralow temperature preservation of fish sperm |
| CN102077823A (en) * | 2010-11-16 | 2011-06-01 | 佛山市生生水产股份有限公司 | Method for cryopreservation of semen of siniperca scherzeri and artificial insemination |
-
2014
- 2014-11-28 CN CN201410714797.1A patent/CN104396943A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001028326A1 (en) * | 1999-10-20 | 2001-04-26 | Istituto Sperimentale Italiano 'lazzaro Spallanzani' | Method for cryopreservation of teleostei semen |
| CN1596670A (en) * | 2004-07-27 | 2005-03-23 | 中国水产科学研究院黄海水产研究所 | Practicalization method for frozen preserving sperm of fish |
| CN101088511A (en) * | 2007-02-15 | 2007-12-19 | 中国水产科学研究院长江水产研究所 | Simple method for ultralow temperature preservation of fish sperm |
| CN102077823A (en) * | 2010-11-16 | 2011-06-01 | 佛山市生生水产股份有限公司 | Method for cryopreservation of semen of siniperca scherzeri and artificial insemination |
Non-Patent Citations (1)
| Title |
|---|
| F.C. WITHLER: "CRYOPRESERVATION OF SPERMATOZOA OF SOME FRESHWATER FISHES CULTURED IN SOUTH AND SOUTHEAST ASIA", 《AQUACULTURE》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104814005A (en) * | 2015-03-26 | 2015-08-05 | 谢光玉 | Preservation method for seminal fluid of Schizothorax davidi |
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Application publication date: 20150311 |