CN104383314A - Medicine for treating epidemic keratitis - Google Patents

Abstract

The invention provides a medicine for treating epidemic keratitis. The medicine is prepared from the following raw medicines: radix bupleuri, radices sileris, cordate houttuynia, fruit of princesplume ladysthumb, trollflower, folium isatidis, radix isatidis, herba violae, astragalus membranaceus, felwort, fructus viticis, concha haliotidis, rhizoma anemarrhenae, caulis sinomenii, radix ophiopogonis, rainbow conk, eleusine indica, mosla scabra, aleppo avens and talinum crassifolium. The medicine is prepared into injection, simple in medication method, free of toxic and side effects, quickly effective, strong in effect and long in lasting time.

Description

Be used for the treatment of the medicine of epidemic keratitis

Technical field

The present invention relates to technical field of Chinese medicine, particularly relate to a kind of medicine being used for the treatment of epidemic keratitis.

Background technology

Epidemic keratoconjunctivitis is one once pandemic eye infectious disease in the whole world.Its pathological change mainly infiltrates under acute follicular or pseudomembranous conjunctivitis and corneal epithelial cell.Primary disease, caused by adenovirus, is communicated by contact, and incubation period is 5 ~ 8 days, the morbidity of normal eyes, and start to invade simple eye, after 2 ~ 7 days, another eye is fallen ill then.From the beginning of time conjunctiva there is congestion and edema suddenly, especially obvious at semilunar fold place, have foreign body sensation, burn feeling and watery secretion; Usually occurring folliculus in morbidity the 3rd day palpebral conjunctiva, increase sharply, is that preauricular lymph nodes enlargement also has tenderness at most with upper and lower fornix portion; Fall ill after 8 ~ 10 days, there is corneal injury and with obvious photophobia, shed tears and blurred vision.

Viral keratitis is adenovirus, conventional antiviral drugs eye drip and systemic administration, but these courses of pharmaceuticals are long, poor effect, and often point turbidity under merging corneal epithelium, affects one's power of vision.

Primary disease belongs to the traditional Chinese medical science " acute and chronic conjunctivitis ", " sudden and violent nebula " category, and how attack because evil poison in season is outer, pyretic toxicity accumulates in lung liver, inside and outside combined pathogens is attacked caused by the order." Gujin Yitong Daquan, Medical Complete Book-ophthalmology " is said: suffer from eye red and swell, lacrimation and pain, or cause all pains of neck, gradually raw cataracta, cover pupil of the eye, purple does not fall apart.The etiology and pathogenesis of this disease mostly is diseases caused by exogenous pathogenic factor epidemic miasma, the high Sheng of interior double lung-fire, invades Liver Channel, above attacks and cause a disease in order.Take turns theory according to the traditional Chinese medical science five, white of the eye belongs to lung, and lung meridian has heat then hyperemia of the bulbar conjunctiva edema.Cornea and iris associating with kidney, flaming up of liver-fire is black eyeball punctate keratitis then, order gritty photophobia.This disease can simple eye or eyes occur simultaneously, and without obviously seasonal, each age group all can be fallen ill.Should based on heat-clearing and toxic substances removing, eliminating evil nebula removing in treatment.Primary disease is with obvious General Symptoms, and therefore systemic administration is real is necessary.

The present invention, based on Chinese traditional treatment theory, provides a kind of injection being used for the treatment of epidemic keratitis, adopts this administrated method simple, has no side effect, and rapid-action, effect is strong, length of holding time.

Summary of the invention

Technical problem to be solved by this invention is to provide a kind of medicine being used for the treatment of epidemic conjunctivitis, is prepared into injection, adopts this administrated method simple, has no side effect, and rapid-action, effect is strong, length of holding time.

For solving the problems of the technologies described above, the invention provides a kind of medicine being used for the treatment of epidemic conjunctivitis, its crude drug comprises Radix Bupleuri, Radix Saposhnikoviae, Herba Houttuyniae, Fructus Polygoni Orientalis, Flos Trollii, Folium Isatidis, Radix Isatidis, Herba Violae, the Radix Astragali, Radix Gentianae, Fructus Viticis, Concha Haliotidis, the Rhizoma Anemarrhenae, Caulis Sinomenii, Radix Ophiopogonis, Coriolous Dersicolor (Fr.) Quel, Herba Eleusines Indicae, Herba Moslae Soochowensis, Radix seu Herba Gei aleppici and Radix Talini Paniculati.

Wherein, in described medicine the weight of each crude drug be respectively Radix Bupleuri 20g ~ 30g, Radix Saposhnikoviae 15g ~ 25g, Herba Houttuyniae 15g ~ 25g, Fructus Polygoni Orientalis 35g ~ 45g, Flos Trollii 30g ~ 40g, Folium Isatidis 55g ~ 65g, Radix Isatidis 30g ~ 40g, Herba Violae 25g ~ 35g, Radix Astragali 25g ~ 35g, Radix Gentianae 30g ~ 40g, Fructus Viticis 10g ~ 20g, Concha Haliotidis 15g ~ 25g, Rhizoma Anemarrhenae 25g ~ 35g, Caulis Sinomenii 30g ~ 40g, Radix Ophiopogonis 25g ~ 35g, Coriolous Dersicolor (Fr.) Quel 15g ~ 25g, Herba Eleusines Indicae 15g ~ 25g, Herba Moslae Soochowensis 20g ~ 30g, Radix seu Herba Gei aleppici 30g ~ 40g and Radix Talini Paniculati 25g ~ 35g.

Wherein, in described medicine the weight of each crude drug be respectively Radix Bupleuri 23g ~ 27g, Radix Saposhnikoviae 18g ~ 22g, Herba Houttuyniae 18g ~ 22g, Fructus Polygoni Orientalis 38g ~ 42g, Flos Trollii 33g ~ 37g, Folium Isatidis 58g ~ 62g, Radix Isatidis 33g ~ 37g, Herba Violae 28g ~ 32g, Radix Astragali 28g ~ 32g, Radix Gentianae 33g ~ 37g, Fructus Viticis 13g ~ 17g, Concha Haliotidis 18g ~ 22g, Rhizoma Anemarrhenae 28g ~ 32g, Caulis Sinomenii 33g ~ 37g, Radix Ophiopogonis 28g ~ 32g, Coriolous Dersicolor (Fr.) Quel 18g ~ 22g, Herba Eleusines Indicae 18g ~ 22g, Herba Moslae Soochowensis 23g ~ 27g, Radix seu Herba Gei aleppici 33g 37g and Radix Talini Paniculati 28g ~ 32g.

Wherein, in described medicine the weight of each crude drug be respectively Radix Bupleuri 25g, Radix Saposhnikoviae 20g, Herba Houttuyniae 20g, Fructus Polygoni Orientalis 40g, Flos Trollii 35g, Folium Isatidis 60g, Radix Isatidis 35g, Herba Violae 30g, Radix Astragali 30g, Radix Gentianae 35g, Fructus Viticis 15g, Concha Haliotidis 20g, Rhizoma Anemarrhenae 30g, Caulis Sinomenii 35g, Radix Ophiopogonis 30g, Coriolous Dersicolor (Fr.) Quel 20g, Herba Eleusines Indicae 20g, Herba Moslae Soochowensis 25g, Radix seu Herba Gei aleppici 35g and Radix Talini Paniculati 30g.

Wherein, the dosage form of described medicine is injection.

The invention provides the preparation method of said medicine, be specially:

The first step, by Herba Houttuyniae, Fructus Polygoni Orientalis, Flos Trollii, Folium Isatidis, Radix Isatidis, Herba Violae, the Radix Astragali, Radix Gentianae, Concha Haliotidis, the Rhizoma Anemarrhenae, Radix Ophiopogonis, Radix seu Herba Gei aleppici and Radix Talini Paniculati are uniformly mixed according to above-mentioned weight, mixture after mix homogeneously being dissolved in determining alcohol is in the ethanol of 85% ~ 95%, the addition of the ethanol that every 100g mixture is corresponding is 300g, flood 24 hours ~ 36 hours, adopt percolation with the speed of 1 ~ 2ml per minute slowly percolation subsequently, collect percolate, percolate is evaporated to about 1/10 of original volume, adopt centrifuge, obtain supernatant, except desolventizing, pulverize and drying, obtain the first powder,

Part II, by remaining components by above-mentioned weight mixing, obtain mixture, add the water relative to mixture quality 2 ~ 4 times, decoct 3 ~ 5 hours, decoction liquor is filtered, and filtering residue adds the water relative to the mixture quality prepared 2 ~ 4 times again above, decoct 2 ~ 4 hours, decoction liquor is filtered, and twice decoction liquor is merged, add concentration be 10% salt acid for adjusting pH value be 3 ~ 4, static 2 ~ 4 hours, filter, filtrate alcohol extraction, extract concentrating under reduced pressure becomes dry mastic, dry, pulverize into the second powder subsequently;

3rd step, the fine powder mixing first step and second step obtained, gets 250g, add appropriate water for injection, stir 20min ~ 40min, adjust ph to 6.0 ~ 7.0, cold preservation 24 hours at the temperature of 0 DEG C ~ 4 DEG C, with 0.45 μm of membrane filtration, add water for injection to 1000ml, then use 0.45 μm of membrane filtration, fill is in injection bottle, sterilizing 30 minutes at 110 DEG C, obtains injection.

Beneficial effect of the present invention:

The medicine being used for the treatment of epidemic conjunctivitis provided by the invention is prepared into injection, adopts this injection administrated method simple, has no side effect, and rapid-action, effect is strong, length of holding time.

Detailed description of the invention

The invention provides a kind of medicine being used for the treatment of epidemic conjunctivitis, its crude drug comprises Radix Bupleuri, Radix Saposhnikoviae, Herba Houttuyniae, Fructus Polygoni Orientalis, Flos Trollii, Folium Isatidis, Radix Isatidis, Herba Violae, the Radix Astragali, Radix Gentianae, Fructus Viticis, Concha Haliotidis, the Rhizoma Anemarrhenae, Caulis Sinomenii, Radix Ophiopogonis, Coriolous Dersicolor (Fr.) Quel, Herba Eleusines Indicae, Herba Moslae Soochowensis, Radix seu Herba Gei aleppici and Radix Talini Paniculati.

Further, described medicine is only made up of above-mentioned raw materials medicine.

In described medicine, the weight of each crude drug is respectively Radix Bupleuri 20g ~ 30g, Radix Saposhnikoviae 15g ~ 25g, Herba Houttuyniae 15g ~ 25g, Fructus Polygoni Orientalis 35g ~ 45g, Flos Trollii 30g ~ 40g, Folium Isatidis 55g ~ 65g, Radix Isatidis 30g ~ 40g, Herba Violae 25g ~ 35g, Radix Astragali 25g ~ 35g, Radix Gentianae 30g ~ 40g, Fructus Viticis 10g ~ 20g, Concha Haliotidis 15g ~ 25g, Rhizoma Anemarrhenae 25g ~ 35g, Caulis Sinomenii 30g ~ 40g, Radix Ophiopogonis 25g ~ 35g, Coriolous Dersicolor (Fr.) Quel 15g ~ 25g, Herba Eleusines Indicae 15g ~ 25g, Herba Moslae Soochowensis 20g ~ 30g, Radix seu Herba Gei aleppici 30g ~ 40g and Radix Talini Paniculati 25g ~ 35g.

Further preferably, in described medicine, the weight of each crude drug is respectively Radix Bupleuri 23g ~ 27g, Radix Saposhnikoviae 18g ~ 22g, Herba Houttuyniae 18g ~ 22g, Fructus Polygoni Orientalis 38g ~ 42g, Flos Trollii 33g ~ 37g, Folium Isatidis 58g ~ 62g, Radix Isatidis 33g ~ 37g, Herba Violae 28g ~ 32g, Radix Astragali 28g ~ 32g, Radix Gentianae 33g ~ 37g, Fructus Viticis 13g ~ 17g, Concha Haliotidis 18g ~ 22g, Rhizoma Anemarrhenae 28g ~ 32g, Caulis Sinomenii 33g ~ 37g, Radix Ophiopogonis 28g ~ 32g, Coriolous Dersicolor (Fr.) Quel 18g ~ 22g, Herba Eleusines Indicae 18g ~ 22g, Herba Moslae Soochowensis 23g ~ 27g, Radix seu Herba Gei aleppici 33g 37g and Radix Talini Paniculati 28g ~ 32g.

Most preferably, in described medicine the weight of each crude drug be respectively Radix Bupleuri 25g, Radix Saposhnikoviae 20g, Herba Houttuyniae 20g, Fructus Polygoni Orientalis 40g, Flos Trollii 35g, Folium Isatidis 60g, Radix Isatidis 35g, Herba Violae 30g, Radix Astragali 30g, Radix Gentianae 35g, Fructus Viticis 15g, Concha Haliotidis 20g, Rhizoma Anemarrhenae 30g, Caulis Sinomenii 35g, Radix Ophiopogonis 30g, Coriolous Dersicolor (Fr.) Quel 20g, Herba Eleusines Indicae 20g, Herba Moslae Soochowensis 25g, Radix seu Herba Gei aleppici 35g and Radix Talini Paniculati 30g.

The pharmacology of described each crude drug is as follows:

Radix Bupleuri: latin name bupleuri radix, for the dry root of umbelliferae bupleurum or Radix Bupeuri Scorzonerfolii., cold nature, bitter in the mouth, pungent, return Liver Channel, gallbladder meridian, there is effect of heat radiation, sterilization, cure mainly cold, fever, alternate attack of chill and fever, malaria, stagnation of QI due to depression of the liver, sternal rib pain, proctoptosis, the cards such as uterus comes off, menoxenia.

Radix Saposhnikoviae: acrid in the mouth, sweet, slightly warm in nature, enters lung, liver, spleen, urinary bladder channel, has expelling cold and relieving exterior syndrome, removing dampness to relieve pain, expelling wind and relieving convulsion, antipruritic effect, is mainly used in affection of exogenous wind-cold card and cold-evil's closing, the syndrome of superficies cold and interior heat etc. of interior-heat pent-up.

Herba Houttuyniae: latin name houttuyniae herba, acrid in the mouth, cold nature, enters lung, urinary bladder channel, has heat-clearing and toxic substances removing, removing pus and relieving carbuncle, effect of inducing diuresis for treating stranguria syndrome, and for lung abscess vomiting pus, expectorant heat syndrome cough is breathed heavily, toxic-heat and blood stasis, damp-heat gonorrhoea, pyretic toxicity carbuncle sore, and snakeworm wants first-class card.

Fructus Polygoni Orientalis: taste becomes, and cold nature, returns liver, stomach warp, have effect of promoting flow of QI and blood, hard masses softening and resolving, cure mainly chronic hepatitis, cirrhotic ascites, the tuberculosis of cervical lymph nodes, splenomegaly, dyspepsia, abdomen amine is had a stomachache, food stagnation of child, the cards such as conjunctivitis.

Flos Trollii: latin name Trollius chinensis, bitter in the mouth, cool in nature, there is effect of heat-clearing and toxic substances removing, cure mainly acute and chronic tonsillitis, acute otitis media, acute myringitis, acute conjunctivitis, the cards such as acute lymphangitis.

Folium Isatidis: bitter in the mouth, property Great Cold, enter the heart, liver, gallbladder, stomach warp, have antipyretic and detoxicated, cooling blood and dissolving purpura, the effect of jaundice eliminating, can the excess-heat that divides of clearing heat in QI system, and can separate again the pyretic toxicity of blood system, the high febrile perspiration being mainly used in pestilence calentura caused goes out, excessive thirst taking fluids profusely; The speckle that fire-toxin is flourishing is gone mad, the symptoms such as haematemesis; The unconsciousness due to high fever of measles, rash color is purple dark; Dampness-heat in the liver and gallbladder accumulates the jaundice of steaming; The deafness and tinnitus of liver and gall fire liter; The card such as pharyngalgia, aphtha of the mouth and tongue, hepatitis that fire stagnation pyretic toxicity is caused.

Radix Isatidis: latin name Isatidis Radix, bitter in the mouth, cold in nature, enter the heart, liver, gallbladder, stomach warp, have the effect of heat-clearing and toxic substances removing, removing heat from blood sore-throat relieving, be used for the treatment of maculae caused by violent heat pathogen; High heat headache; Major part pestilence; Crimson tongue is purple dark, scarlet fever; Erysipelas; Mumps; Sore throat; Skin ulcer is swollen, carbuncle; Chickenpox; Measles; Hepatitis; Influenza, epidemic encephalitis, encephalitis b, pneumonia, coma tells nosebleed, pharyngeal swelling, acute catarrhal conjunctivitis, skin ulcer rash; The cards such as epidemic encephalitis type B, acute, chronic hepatitis, mumps, osteomyelitis can be prevented and treated.

Herba Violae: bitter in the mouth, pungent, cold in nature, enter the heart, Liver Channel, there is the effect of heat-clearing and toxic substances removing, removing heat from blood detumescence, be mainly used in the treatment for the treatment of furuncle carbuncle furuncle, also have good therapeutic effect to acute conjunctivitis, hordeolum etc., Herba Violae has inhibitory action in various degree to Staphyloccocus aureus, pneumobacillus, escherichia coli, alpha streptococcus, group B streptococcus, hemophilus influenza, diphtheria corynebacterium, bacillus pyocyaneus, white grapes sugar coccus, Candida albicans.

The Radix Astragali: latin name astragali radix, sweet in the mouth, warm in nature, return lung, spleen channel, have tonification spleen soil, elevate a turnable ladder yang-energy, strengthening superficial resistance to stop perspiration, the effects such as holder skin ulcer granulation promoting, are mainly used in treating the caused weak anorexia and loose stool of deficiency of both the splenic and pulmonary QI, shortness of breath and palpitation, the visceroptosis of sinking of QI of middle-JIAO, the metrorrhagia of QI failing to control blood is had blood in stool, chronic diarrhea proctoptosis; The spontaneous sweating of exterior deficiency; Insufficiency of vital energy and blood, yang-energy skin infection on the decline does not rise, difficulty in discharging of pus, burst and do not hold back for a long time; And the deficiency of vital energy loses the edema of fortune, the symptoms such as dysuria.

Radix Gentianae: bitter in the mouth, cold in nature, enters liver, gallbladder meridian, has heat clearing and damp drying, falls the effect of letting out liver-fire and function of gallbladder promoting, and the breast side of body being mainly used in dampness-heat of the liver caused is had a pain, and bitter taste is deaf, conjunctival congestion and swelling pain; Jaundice due to damp-heat; The dizzy order of flaming up of liver-fire swells; The high heat of liver-fire wind-transformation is through taking out; The card such as leucorrhea, dysuria of damp invasion of lower energizer.

Fructus Viticis: acrid in the mouth, hardship, cold nature, returns bladder, liver, stomach warp, has dispelling wind and heat pathogens, effect of the clear sharp head, cures mainly anemopyretic cold headache, gingivitis, the many tear of conjunctival congestion, poor vision, the card such as to have a dizzy spell.

Concha Haliotidis: taste becomes, and property is put down, and has effect of suppressing the hyperactive liver to relieve the wind syndrome, YANG hyperactivity suppressing, heat extraction improving eyesight, cures mainly the diseases such as excessive rising of liver-YANG, the vertigo, asthenia hectic fever due to YIN-deficiency, haematemesis, optic atrophy.

The Rhizoma Anemarrhenae: bitter in the mouth, sweet, cold in nature, enters lung, stomach, kidney channel, has relieving restlessness of purging heat, the effect of nourshing Yin and drynsessmoistening prescription, clearing away lung-heat to relieve cough, and cure mainly fever caused by exogenous pathogenic factors, high heat is irritated, lung-heat type cough, and interior-heat such as to be quenched one's thirst at the symptom; Its bitter cold matter is moistened, and property sedimentation can heat clearing away, can promote the production of body fluid again, kindly controls warm pathogenic factor and enters edema caused by disorder of QI, intenseness of heat impairment of body fluid; Also diabetes is used for the treatment of; Phlegm-heat obstructing in the lung and breathing heavily, xeropulmonary cough, expectorant is few unfavorable; Hyperactivity of fire caused by deficiency of YIN, the symptoms such as hyperactivity of deficient fire.

Caulis Sinomenii: bitter in the mouth, pungent, the cards such as property is put down, and return liver, spleen channel, have wind-damp dispelling, the meridian dredging, effect of diuresis, cures mainly rheumatic arthralgia, arthroncus, paralysis pruritus.

Radix Ophiopogonis: sweet in the mouth, micro-hardship, cold nature, enters the heart, lung, stomach warp, there is nourishing YIN and moistening the lung, effect that preventing phlegm from forming and stopping coughing, heat clearing away are nourished heart, cure mainly pathogenic dryness impairing the lung or lung-dryness syndrome due to deficiency of YIN has the dry cough expectorant of heat to stick, chronic cough hemoptysis, dry pharynx dryness in the nasal cavity, lung abscess, thermal burn stomach-Yin or the dry mouth and throat caused by deficiency of stomach-Yin, the few tongue of red tongue, vague epigastralgia, interior-heat is quenched one's thirst, constipation due to dry stool, deficiency of heart-yin and epidemic febrile disease pathogenic heat are disturbed and the heart-nutriment and the dysphoria and insomnia that causes, crimson tongue and dryly wait card.

Coriolous Dersicolor (Fr.) Quel: sweet in the mouth, light, cold nature, returns liver; Spleen; Lung meridian, has invigorating spleen to remove dampness; Relieving cough and asthma; Heat-clearing and toxic substances removing; Antineoplastic effect, cures mainly chronic active hepatitis; Hepatitis interstitialis chronica; Chronic bronchitis; Infantile spasm bronchitis; Laryngopharynx swelling and pain; Kinds of tumors; Rheumatoid arthritis; The cards such as leukemia.

Herba Eleusines Indicae: be root or the herb of grass family yard grass platymiscium Herba Eleusines Indicae, sweet in the mouth, light, cool in nature, there is heat-clearing and toxic substances removing, dampness removing, effect of cool the blood dissipate blood stasis, cure mainly Sunstroke heating, infantile convulsion, encephalitis b, epidemic encephalitis, jaundice, stranguria; dysuria, dysentery, has blood in stool, urticaria, the cards such as traumatic injury.

Herba Moslae Soochowensis: warm in nature, acrid in the mouth, has dispelling summer-heat from superficies of the body, and effect of regulating QI to relieve pain, cures mainly flu, heatstroke, eruptive disease, peratodynia, the cards such as laryngopharynx swelling and pain.

Radix seu Herba Gei aleppici: latin name Gei Herba, acrid in the mouth, hardship, property is put down, and return liver, spleen, lung meridian, have analgesia, blood pressure lowering, regulating menstruation, effect of expelling wind and removing dampness, cures mainly hypertension, dizziness headache, menoxenia, lower abdominal pain, leucorrhea, infantile convulsion, lumbago, skelalgia of rheumatism; Carbuncle furuncle and phyma poison is controlled in external, the cards such as traumatic injury.

Radix Talini Paniculati: latin name Pedicularis muscicola Maxim, sweet in the mouth, micro-hardship, tool heat-clearing toxin-expelling functions, weak to the deficiency of vital energy, diarrhea due to hypofunction of the spleen, xeropulmonary cough, neurasthenia etc. have certain curative effect.Strongly invigorating primordial QI, promoting the production of body fluid and inducing sedation of the mind, heart tonifying.Control QI and blood deficient, asthenia hyperhidrosis, collapse exhaustion, blood pressure reduces.

Because primary disease is with obvious General Symptoms, therefore systemic administration is real is necessary.Therefore, medicine provided by the invention is based on systemic administration, and the dosage form provided is injection.

The invention provides the preparation method of said medicine, be specially:

The first step, by Herba Houttuyniae, Fructus Polygoni Orientalis, Flos Trollii, Folium Isatidis, Radix Isatidis, Herba Violae, the Radix Astragali, Radix Gentianae, Concha Haliotidis, the Rhizoma Anemarrhenae, Radix Ophiopogonis, Radix seu Herba Gei aleppici and Radix Talini Paniculati are uniformly mixed according to above-mentioned weight, mixture after mix homogeneously being dissolved in determining alcohol is in the ethanol of 85% ~ 95%, the addition of the ethanol that every 100g mixture is corresponding is 300g, flood 24 hours ~ 36 hours, adopt percolation with the speed of 1 ~ 2ml per minute slowly percolation subsequently, collect percolate, percolate is evaporated to about 1/10 of original volume, adopt centrifuge, obtain supernatant, except desolventizing, pulverize and drying, obtain the first powder,

Part II, by remaining components by above-mentioned weight mixing, obtain mixture, add the water relative to mixture quality 2 ~ 4 times, decoct 3 ~ 5 hours, decoction liquor is filtered, and filtering residue adds the water relative to the mixture quality prepared 2 ~ 4 times again above, decoct 2 ~ 4 hours, decoction liquor is filtered, and twice decoction liquor is merged, add concentration be 10% salt acid for adjusting pH value be 3 ~ 4, static 2 ~ 4 hours, filter, filtrate alcohol extraction, extract concentrating under reduced pressure becomes dry mastic, dry, pulverize into the second powder subsequently;

3rd step, the powder mixing first step and second step obtained, gets 250g, add appropriate water for injection, stir 20min ~ 40min, adjust ph to 6.0 ~ 7.0, cold preservation 24 hours at the temperature of 0 DEG C ~ 4 DEG C, with 0.45 μm of membrane filtration, add water for injection to 1000ml, then use 0.45 μm of membrane filtration, fill is in injection bottle, sterilizing 30 minutes at 110 DEG C, obtains injection.

Below adopt embodiment to describe embodiments of the present invention in detail, to the present invention, how application technology means solve technical problem whereby, and the implementation procedure reaching technique effect can fully understand and implement according to this.

Embodiment 1 injection 1

By Herba Houttuyniae 20g, Fructus Polygoni Orientalis 40g, Flos Trollii 35g, Folium Isatidis 60g, Radix Isatidis 35g, Herba Violae 30g, Radix Astragali 30g, Radix Gentianae 35g, Concha Haliotidis 20g, Rhizoma Anemarrhenae 30g, Radix Ophiopogonis 30g, Radix seu Herba Gei aleppici 35g and Radix Talini Paniculati 30g is uniformly mixed, and the mixture after mix homogeneously being dissolved in determining alcohol is in the ethanol of 95%, and the addition of the ethanol that every 100g mixture is corresponding is 300g, flood 36 hours, adopt percolation with the speed of 2ml per minute slowly percolation subsequently, collect percolate, percolate is evaporated to about 1/10 of original volume, adopt centrifuge, obtain supernatant, except desolventizing, pulverize and drying, obtain the first powder, by Radix Bupleuri 25g, Radix Saposhnikoviae 20g, Fructus Viticis 15g, Caulis Sinomenii 35g, Coriolous Dersicolor (Fr.) Quel 20g, Herba Eleusines Indicae 20g, Herba Moslae Soochowensis 25g mixes, and obtains mixture, adds the water of 450g, decoct 4 hours, decoction liquor is filtered, and filtering residue adds the water of 450g again, decoct 3 hours, decoction liquor is filtered, and twice decoction liquor is merged, add concentration be 10% salt acid for adjusting pH value be 3, static 3 hours, filter, filtrate alcohol extraction, extract concentrating under reduced pressure becomes dry mastic, dry, pulverize into the second powder subsequently, by the powder mixing obtained above, get 250g, add appropriate water for injection, stir 30min, adjust ph to 7.0, cold preservation 24 hours at the temperature of 0 DEG C, with 0.45 μm of membrane filtration, adds water for injection to 1000ml, use 0.45 μm of membrane filtration again, fill is in injection bottle, and sterilizing 30 minutes at 110 DEG C, obtains injection 1.

Embodiment 2 injection 2

By Herba Houttuyniae 18g, Fructus Polygoni Orientalis 38g, Flos Trollii 33g, Folium Isatidis 58g, Radix Isatidis 33g, Herba Violae 28g, Radix Astragali 28g, Radix Gentianae 33g, Concha Haliotidis 18g, Rhizoma Anemarrhenae 28g, Radix Ophiopogonis 28g, Radix seu Herba Gei aleppici 33g and Radix Talini Paniculati 28g is uniformly mixed, and the mixture after mix homogeneously being dissolved in determining alcohol is in the ethanol of 95%, and the addition of the ethanol that every 100g mixture is corresponding is 300g, flood 36 hours, adopt percolation with the speed of 2ml per minute slowly percolation subsequently, collect percolate, percolate is evaporated to about 1/10 of original volume, adopt centrifuge, obtain supernatant, except desolventizing, pulverize and drying, obtain the first powder, by Radix Bupleuri 23g, Radix Saposhnikoviae 18g, Fructus Viticis 13g, Caulis Sinomenii 33g, Coriolous Dersicolor (Fr.) Quel 18g, Herba Eleusines Indicae 18g, Herba Moslae Soochowensis 23g mixes, and obtains mixture, adds the water of 450g, decoct 4 hours, decoction liquor is filtered, and filtering residue adds the water of 450g again, decoct 3 hours, decoction liquor is filtered, and twice decoction liquor is merged, add concentration be 10% salt acid for adjusting pH value be 3, static 3 hours, filter, filtrate alcohol extraction, extract concentrating under reduced pressure becomes dry mastic, dry, pulverize into the second powder subsequently, by the powder mixing obtained above, get 250g, add appropriate water for injection, stir 30min, adjust ph to 7.0, cold preservation 24 hours at the temperature of 0 DEG C, with 0.45 μm of membrane filtration, adds water for injection to 1000ml, use 0.45 μm of membrane filtration again, fill is in injection bottle, and sterilizing 30 minutes at 110 DEG C, obtains injection 2.

Embodiment 3 injection 3

By Herba Houttuyniae 22g, Fructus Polygoni Orientalis 42g, Flos Trollii 37g, Folium Isatidis 62g, Radix Isatidis 37g, Herba Violae 32g, Radix Astragali 32g, Radix Gentianae 37g, Concha Haliotidis 22g, Rhizoma Anemarrhenae 32g, Radix Ophiopogonis 32g, Radix seu Herba Gei aleppici 37g and Radix Talini Paniculati 32g is uniformly mixed, and the mixture after mix homogeneously being dissolved in determining alcohol is in the ethanol of 95%, and the addition of the ethanol that every 100g mixture is corresponding is 300g, flood 36 hours, adopt percolation with the speed of 2ml per minute slowly percolation subsequently, collect percolate, percolate is evaporated to about 1/10 of original volume, adopt centrifuge, obtain supernatant, except desolventizing, pulverize and drying, obtain the first powder, by Radix Bupleuri 27g, Radix Saposhnikoviae 22g, Fructus Viticis 17g, Caulis Sinomenii 37g, Coriolous Dersicolor (Fr.) Quel 22g, Herba Eleusines Indicae 22g, Herba Moslae Soochowensis 27g mixes, and obtains mixture, adds the water of 450g, decoct 4 hours, decoction liquor is filtered, and filtering residue adds the water of 450g again, decoct 3 hours, decoction liquor is filtered, and twice decoction liquor is merged, add concentration be 10% salt acid for adjusting pH value be 3, static 3 hours, filter, filtrate alcohol extraction, extract concentrating under reduced pressure becomes dry mastic, dry, pulverize into the second powder subsequently, by the powder mixing obtained above, get 250g, add appropriate water for injection, stir 30min, adjust ph to 7.0, cold preservation 24 hours at the temperature of 0 DEG C, with 0.45 μm of membrane filtration, adds water for injection to 1000ml, use 0.45 μm of membrane filtration again, fill is in injection bottle, and sterilizing 30 minutes at 110 DEG C, obtains injection 3.

Embodiment 4 toxicology test

General pharmacology is tested

To injection 1 100ml/kg prepared by the present invention of sobering animal K.M kind injected in mice, observe 7 days, result voluntary activity is normal, Neurotoxicity is without obvious excited or suppression phenomenon, hair is smooth, diet, feces no abnormality seen, illustrate that injection of the present invention all has no side effect to nervous system, cardiovascular system respiratory system of unifying.

3, long term toxicity test: domesticated dog 60, injection group 40, often organize 20, injection 0.2/kg and lg/kg90 days of the continuous intravenous injection embodiment of the present invention 1 preparation respectively, matched group is not injected, experimental session, and each treated animal has no poisoning symptom, activity, behavior, diet, feces no abnormality seen, body weight increases with matched group close; Within 10 days, measure electrocardiogram, hematology, blood biochemical, urinalysis 25 test items respectively after 45 days and 90 days and drug withdrawal before administration, after administration, result medication two groups is basically identical with matched group; Medication after 90 days and after drug withdrawal 10 days, to heavy dose of 8, middle dosage 6, matched group 6, carries out 12 organ coefficients and 25 histology's microscopies, is showed no exception; Animal behavior no abnormality seen after drug withdrawal, illustrates that injection of the present invention is without savings intoxication.

Injection 1 prepared by the embodiment of the present invention 1 under 0.2g/kg and 1.0g/kg over much dosage (than the large 35-175 of clinical dosage doubly, than the large 20-100 of animal effective dose doubly) to domesticated dog continuous intravenous injection 90 days, observation is continued 10 days after drug withdrawal, experimental session, each treated animal has been showed no poisoning symptom.The activity of animal, behavior, diet, feces have no abnormal changes.Body weight growth pattern close with matched group (P > 0.05).Before in after and when checking for four times after drug withdrawal, medication two groups and matched group are basically identical.Pathological examination, medication is after 90 days and drug withdrawal after 10 days, and check heavy dose of 8 altogether, middle dosage 6, matched group 6,12 organ coefficients and 25 histology's microscopies, be showed no exception.Prompting: liver is likely toxicity target organ.After drug withdrawal, animal behavior has no morbidity phenomenon, and within the 10th day, provocative test result is negative.

Above-mentioned experimental data shows: Chinese medicine injection long term toxicity of the present invention is very little, is very safe.

Embodiment 5 clinical data

Physical data

Epidemic keratitis patient totally 159 examples that in October, 2012 ~ 2013 year December is accepted for medical treatment, ill eye number is 261, and patient age is minimum is 8 years old, and be 69 years old to the maximum, disease time the shortest is 2 days, and the longest is 28 days.Patient is divided into treatment group and matched group at random, treatment group patient 80 example, ill eye number is 135, matched group patient 79 example, several 126 of ill eye.

Diagnostic criteria

Two groups of case diagnosis are all according to " Chinese medical disease Standardization of diagnosis and curative effect " that State Administration of Traditional Chinese Medicine issues.

Therapeutic Method

The injection 1 that treatment group adopts the embodiment of the present invention 1 to prepare, every bottle of 100ml, adds 5% glucose injection 200ml at every turn and dilutes latter quiet by 100ml injection 1,30 per minute, every day 1 time, inject 7 days, matched group quiet Ribavirin presses the consumption of 70mg/kg, every day 1 time.According to pharmacopoeial requirements, limit the use of 7 days.

Two groups all use 50% Herba Houttuyniae eye liquid and 0.5% virazole eye drop to suffer from eye, every day 5 times, and oral vitamin C 0.2g and compound vitamin B while injection ₂sheet, every day 3 times.

Criterion of therapeutical effect

Efficacy evaluation is with reference to " Chinese medical disease Standardization of diagnosis and curative effect ".Cure: subjective symptoms disappears, eyelid, bulbar conjunctiva are without hyperemia, edema and folliculus, and corneal fluorescein dyeing is for negative.Take a turn for the better: subjective symptoms is slight, eyelid, bulbar conjunctiva mild hyperaemia, corneal fluorescein dyeing is (-) or (±).Do not heal: the eye state of an illness is unchanged or increase the weight of.

Statistical method

Measurement data adopts represent, compare between group and adopt χ ²inspection, all data all adopt the process of SPSS12.0 statistical software.

Result

Two groups of general curative effects compare, and the results are shown in Table 1.

Table 1 liang group overall patient comparitive study

Group	N (eye)	Cure	Take a turn for the better	Invalid	Cure rate/%
						Treatment group	135	112	23	0	82.9
Matched group	126	82	44	0	65.1

As can be seen from Table 1, adopt the therapeutic effect of medicine provided by the invention will significantly better than the therapeutic effect of matched group medicine, p < 0.05.

Cure patient treatment Annual distribution, in table 2 for two groups.

A table 2 liang group cures patient treatment Annual distribution

Group	N (eye)	1～3d	4～5d	6～7d
					Treatment group	135	63	49	23
Matched group	126	37	43	46

All above-mentioned this intellectual properties of primary enforcement, not setting restriction this new product of other forms of enforcement and/or new method.Those skilled in the art will utilize this important information, and foregoing is revised, to realize similar implementation status.But all modifications or transformation belong to the right of reservation based on new product of the present invention.

The above is only preferred embodiment of the present invention, and be not restriction the present invention being made to other form, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the Equivalent embodiments of equivalent variations.But everyly do not depart from technical solution of the present invention content, any simple modification, equivalent variations and the remodeling done above embodiment according to technical spirit of the present invention, still belong to the protection domain of technical solution of the present invention.

Claims (6)

1. be used for the treatment of a medicine for epidemic conjunctivitis, it is characterized in that: crude drug comprises Radix Bupleuri, Radix Saposhnikoviae, Herba Houttuyniae, Fructus Polygoni Orientalis, Flos Trollii, Folium Isatidis, Radix Isatidis, Herba Violae, the Radix Astragali, Radix Gentianae, Fructus Viticis, Concha Haliotidis, the Rhizoma Anemarrhenae, Caulis Sinomenii, Radix Ophiopogonis, Coriolous Dersicolor (Fr.) Quel, Herba Eleusines Indicae, Herba Moslae Soochowensis, Radix seu Herba Gei aleppici and Radix Talini Paniculati.

2. medicine as claimed in claim 1, it is characterized in that: the weight of each crude drug is respectively Radix Bupleuri 20g ~ 30g, Radix Saposhnikoviae 15g ~ 25g, Herba Houttuyniae 15g ~ 25g, Fructus Polygoni Orientalis 35g ~ 45g, Flos Trollii 30g ~ 40g, Folium Isatidis 55g ~ 65g, Radix Isatidis 30g ~ 40g, Herba Violae 25g ~ 35g, Radix Astragali 25g ~ 35g, Radix Gentianae 30g ~ 40g, Fructus Viticis 10g ~ 20g, Concha Haliotidis 15g ~ 25g, Rhizoma Anemarrhenae 25g ~ 35g, Caulis Sinomenii 30g ~ 40g, Radix Ophiopogonis 25g ~ 35g, Coriolous Dersicolor (Fr.) Quel 15g ~ 25g, Herba Eleusines Indicae 15g ~ 25g, Herba Moslae Soochowensis 20g ~ 30g, Radix seu Herba Gei aleppici 30g ~ 40g and Radix Talini Paniculati 25g ~ 35g.

3. medicine as claimed in claim 1 or 2, it is characterized in that: the weight of each crude drug is respectively Radix Bupleuri 23g ~ 27g, Radix Saposhnikoviae 18g ~ 22g, Herba Houttuyniae 18g ~ 22g, Fructus Polygoni Orientalis 38g ~ 42g, Flos Trollii 33g ~ 37g, Folium Isatidis 58g ~ 62g, Radix Isatidis 33g ~ 37g, Herba Violae 28g ~ 32g, Radix Astragali 28g ~ 32g, Radix Gentianae 33g ~ 37g, Fructus Viticis 13g ~ 17g, Concha Haliotidis 18g ~ 22g, Rhizoma Anemarrhenae 28g ~ 32g, Caulis Sinomenii 33g ~ 37g, Radix Ophiopogonis 28g ~ 32g, Coriolous Dersicolor (Fr.) Quel 18g ~ 22g, Herba Eleusines Indicae 18g ~ 22g, Herba Moslae Soochowensis 23g ~ 27g, Radix seu Herba Gei aleppici 33g 37g and Radix Talini Paniculati 28g ~ 32g.

4. the medicine as described in claims 1 to 3, is characterized in that: the weight of each crude drug be respectively Radix Bupleuri 25g, Radix Saposhnikoviae 20g, Herba Houttuyniae 20g, Fructus Polygoni Orientalis 40g, Flos Trollii 35g, Folium Isatidis 60g, Radix Isatidis 35g, Herba Violae 30g, Radix Astragali 30g, Radix Gentianae 35g, Fructus Viticis 15g, Concha Haliotidis 20g, Rhizoma Anemarrhenae 30g, Caulis Sinomenii 35g, Radix Ophiopogonis 30g, Coriolous Dersicolor (Fr.) Quel 20g, Herba Eleusines Indicae 20g, Herba Moslae Soochowensis 25g, Radix seu Herba Gei aleppici 35g and Radix Talini Paniculati 30g.

5. the medicine as described in Claims 1-4, is characterized in that: the dosage form of described medicine is injection.

6. the preparation method of medicine described in Claims 1-4, is characterized in that, comprising:

The first step, by Herba Houttuyniae, Fructus Polygoni Orientalis, Flos Trollii, Folium Isatidis, Radix Isatidis, Herba Violae, the Radix Astragali, Radix Gentianae, Concha Haliotidis, the Rhizoma Anemarrhenae, Radix Ophiopogonis, Radix seu Herba Gei aleppici and Radix Talini Paniculati are uniformly mixed according to above-mentioned weight, mixture after mix homogeneously being dissolved in determining alcohol is in the ethanol of 85% ~ 95%, the addition of the ethanol that every 100g mixture is corresponding is 300g, flood 24 hours ~ 36 hours, adopt percolation with the speed of 1 ~ 2ml per minute slowly percolation subsequently, collect percolate, percolate is evaporated to about 1/10 of original volume, adopt centrifuge, obtain supernatant, except desolventizing, pulverize and drying, obtain the first powder,

Part II, by component under She by above-mentioned weight mixing, obtain mixture, add the water relative to mixture quality 2 ~ 4 times, decoct 3 ~ 5 hours, decoction liquor is filtered, and filtering residue adds the water relative to the mixture quality prepared 2 ~ 4 times again above, decoct 2 ~ 4 hours, decoction liquor is filtered, and twice decoction liquor is merged, add concentration be 10% salt acid for adjusting pH value be 3 ~ 4, static 2 ~ 4 hours, filter, filtrate alcohol extraction, extract concentrating under reduced pressure becomes dry mastic, dry, pulverize into the second powder subsequently;

3rd step, the fine powder mixing first step and second step obtained, gets 250g, add appropriate water for injection, stir 20min ~ 40min, adjust ph to 6.0 ~ 7.0, cold preservation 24 hours at the temperature of 0 DEG C ~ 4 DEG C, with 0.45 μm of membrane filtration, add water for injection to 1000ml, then use 0.45 μm of membrane filtration, fill is in injection bottle, sterilizing 30 minutes at 110 DEG C, obtains injection.