CN104352826B - Membranous milkvetch stem and leaf is the herbal medicine and preparation method of main ingredient - Google Patents
Membranous milkvetch stem and leaf is the herbal medicine and preparation method of main ingredient Download PDFInfo
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/481—Astragalus (milkvetch)
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
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Abstract
Membranous milkvetch stem and leaf is the herbal medicine and preparation method of main ingredient.A kind of membranous milkvetch stem and leaf is the herbal medicine of main ingredient, is made up of following raw material:Membranous milkvetch stem and leaf, dandelion herb, bracteal leaf of corn, balsampear stem, the parts by weight of described membranous milkvetch stem and leaf are 70 ~ 40, the parts by weight of described dandelion herb are 20 ~ 10, and the parts by weight of described bracteal leaf of corn are 20 ~ 10, and the parts by weight of described balsampear stem are 20 ~ 10.
Description
Technical field：
The present invention relates to herbal medicine and preparation method of a kind of membranous milkvetch stem and leaf for main ingredient.
Background technology：
Membranous milkvetch stem and leaf be used as medicine first recorded in《Mingyi Bielu》, its is sweet, pungent, mild-natured, enter the heart, the warp of liver two, with promoting the production of body fluid to quench thirst,
Relax the muscles and stimulate the blood circulation, the treatment function such as sore of subsiding a swelling, cure mainly quench one's thirst, the thirsty and dry pharynx of consumption of YIN caused by febrile disease, muscle arteries and veins contraction, joint joint stuffiness, carbuncle
The diseases such as subcutaneous ulcer boil.Dandelion herb has the effect such as anti-inflammatory, anti-oxidant, anticancer, antihyperglycemic, antithrombus formation.Bracteal leaf of corn
With reduction artery sclerosis, reduction high fat of blood, also there is preferable Scavenging activity to hydroxyl radical free radical and super radical anion.It is bitter
Melon vine, which has, improves the effect such as body immunity, antitumor, antiviral.These all illustrate to be equipped with Pu by major ingredient of membranous milkvetch stem and leaf
The compound beasts Chinese medicine that public English herb, bracteal leaf of corn, balsampear stem are made has good exploitation prospect.
The content of the invention：
Based on a kind of immunity that can improve beasts, the membranous milkvetch stem and leaf of quickening growth rate
The herbal medicine and preparation method of medicine.
Above-mentioned purpose is realized by following technical scheme：
A kind of membranous milkvetch stem and leaf is the herbal medicine of main ingredient, is made up of following raw material:Membranous milkvetch stem and leaf, dandelion herb, corn bud
Leaf, balsampear stem, the parts by weight of described membranous milkvetch stem and leaf are 70 ~ 40, and the parts by weight of described dandelion herb are 20 ~ 10,
The parts by weight of described bracteal leaf of corn are 20 ~ 10, and the parts by weight of described balsampear stem are 20 ~ 10.
Described membranous milkvetch stem and leaf is the herbal medicine of main ingredient, and the parts by weight of described membranous milkvetch stem and leaf are 60, and described Pu is public
The parts by weight of English herb are 15, and the parts by weight of described bracteal leaf of corn are 15, and the parts by weight of described balsampear stem are 15.
A kind of membranous milkvetch stem and leaf is the preparation method of the herbal medicine of main ingredient, and the first step prepares membranous milkvetch stem and leaf decocting liquid, claimed first
The parts by weight for taking membranous milkvetch stem and leaf are 70 ~ 40, are crushed, and then add 8 times of amount water and soak 20-24 hours, extraction 3 times, often
Secondary 2 h, mixed extract is finally concentrated；
Second step prepares dandelion herb decocting liquid, and the parts by weight that dandelion herb is weighed first are 20 ~ 10, are carried out
Crush, then add 5 times of amount water and soak 20-24 hours, extract 3 times, 2 h, mixed extract, are finally concentrated every time；
3rd step prepares bracteal leaf of corn decocting liquid, and the parts by weight that bracteal leaf of corn is weighed first are 20 ~ 10, are crushed,
Then 10 times of amount water are added to soak 20-24 hours, are extracted 3 times, 2 h, mixed extract, are finally concentrated every time；
4th step prepares balsampear stem decocting liquid, and the parts by weight that balsampear stem is weighed first are 20 ~ 10, are crushed, then
Add 8 times of amount water to soak 20-24 hours, extract 3 times, 2 h, mixed extract, are finally concentrated every time；
5th step is according to membranous milkvetch stem and leaf decocting liquid：Dandelion herb decocting liquid：Bracteal leaf of corn decocting liquid：Balsampear stem decocting liquid
Ratio be 6:2:1:1 ratio mixing, it is membranous milkvetch stem and leaf compound decocting liquid to carry out centrifugal treating and supernatant is made.
Described membranous milkvetch stem and leaf is the preparation method of the herbal medicine of main ingredient, and the first step prepares membranous milkvetch stem and leaf decocting liquid, first
The weight for weighing membranous milkvetch stem and leaf is 50g, is crushed, and then adds 8 times of amount water and soaks 20-24 hour, extraction 3 times, every time 2
H, mixed extract is finally concentrated；
Second step prepares dandelion herb decocting liquid, and the weight that dandelion herb is weighed first is 50g, is crushed, so
5 times of amount water are added afterwards to soak 20-24 hours, are extracted 3 times, 2 h, mixed extract, are finally concentrated every time；
3rd step prepares bracteal leaf of corn decocting liquid, and the weight that bracteal leaf of corn is weighed first is 50g, is crushed, Ran Houjia
Enter 10 times of amount water to soak 20-24 hours, extract 3 times, 2 h, mixed extract, are finally concentrated every time；
4th step prepares balsampear stem decocting liquid, and balsampear stem weight is weighed first for 50g, is crushed, and then adds 8 times of amounts
Water soaks 20-24 hours, extracts 3 times, 2 h, mixed extract, are finally concentrated every time；
5th step is according to membranous milkvetch stem and leaf decocting liquid：Dandelion herb decocting liquid：Bracteal leaf of corn decocting liquid：Balsampear stem decocting liquid
Ratio be 6:2:1:1 ratio mixing, it is membranous milkvetch stem and leaf compound decocting liquid to carry out centrifugal treating and supernatant is made.
Described membranous milkvetch stem and leaf is the preparation method of the herbal medicine of main ingredient, and described is extracted as the extraction decocting of decocting cooking method
Liquid；Described concentration is the 2/3 of the decocting liquid weight for being concentrated into a procedure.
Beneficial effect：
1. the present invention uses membranous milkvetch stem and leaf compound decocting liquid, immune effect of vaccine can be significantly increased, promotes chicken immune device
Official develops, and has directly killing and blocking effect to newcastle disease virus (NDV).
The present invention uses membranous milkvetch stem and leaf compound decocting liquid, can significantly mitigate the cytopathy that virus is caused, and have certain
Dose-effect relationship.
The present invention uses membranous milkvetch stem and leaf compound decocting liquid, can significantly improve beaver rabbit, the coat quality of mink.
The present invention, which has, promotes chicken non-specific immune function, coordinates ND Vaccine to promote specific immune function,
By observing in the interval interior antibody potency change of doses, lymphopoiesis situation, red blood cell yeast rosette rate, immune device
The indexs such as official's index, growth performance.
The present invention is had using membranous milkvetch stem and leaf compound decocting liquid to the In-vitro Inhibitory Effect of newcastle disease virus greatly to be carried
It is high.
The present invention has a significant impact to beaver rabbit, mink hide area, hide weight, the area and weight of hide with
The raising of membranous milkvetch stem and leaf compound decocting liquid consumption has increased trend, illustrates membranous milkvetch stem and leaf compound decocting liquid to beaver rabbit, mink hair
The growth of skin has certain facilitation.
The present invention is that beast in the compound that dandelion herb, bracteal leaf of corn, balsampear stem are made is equipped with by major ingredient of membranous milkvetch stem and leaf
Medicine, using safely, have no side effect.
The present invention is equipped with the compound traditional Chinese veterinary medicine that dandelion herb, bracteal leaf of corn, balsampear stem are made by major ingredient of membranous milkvetch stem and leaf
What is developed can be made all formulations such as oral liquid, injection, tablet, capsule, powder-injection, pill, powder, user
Just, effect is good.
The present invention is that the various active components extracted from membranous milkvetch stem and leaf are major ingredient, dandelion herb, bracteal leaf of corn, balsam pear
The various active components extracted in rattan are the herbal medicine that auxiliary material is developed, and effect is obvious.
The present invention is equipped with dandelion herb, bracteal leaf of corn, hardship using membranous milkvetch stem and leaf as main ingredient
The compound traditional Chinese veterinary medicine that melon vine is made；Radix Astragali tradition agents area is root, and its when being used as medicine a large amount of cauline leafs abandoned it, it is yellow
Containing the plurality of active ingredients such as saponin(e, polysaccharide, amino acid, protein, flavones in stilbene cauline leaf, with improving immune, antibacterial, disease-resistant
The effect such as malicious, anti-oxidant.Dandelion, bracteal leaf of corn, balsampear stem equally have anti-inflammatory, improve body immunity, antitumor, anti-
Virus etc. is acted on.
The present invention is using membranous milkvetch stem and leaf as main ingredient, and dandelion herb, bracteal leaf of corn, balsampear stem are auxiliary material, it is intended to by this compound
Preparation is used for field of traditional Chinese veterinary, is not only effectively protected Radix Astragali resource, realizes the sustainable use of the Radix Astragali, in addition dandelion
Herb, bracteal leaf of corn, the coordination pharmacological action of balsampear stem, more efficiently enhance the immunologic function, disease-resistant of the animals such as birds
The pharmacological activity such as poison, antibacterial, anti-inflammatory, research has shown that this compound preparation can also improve fleece animal pelage quality, enhancing is dynamic
The body weight of thing.
Embodiment：
Embodiment 1：
A kind of membranous milkvetch stem and leaf is the herbal medicine of main ingredient, is made up of following raw material:Membranous milkvetch stem and leaf, dandelion herb, corn bud
Leaf, balsampear stem, the parts by weight of described membranous milkvetch stem and leaf are 70 ~ 40, and the parts by weight of described dandelion herb are 20 ~ 10,
The parts by weight of described bracteal leaf of corn are 20 ~ 10, and the parts by weight of described balsampear stem are 20 ~ 10.
Embodiment 2：
Membranous milkvetch stem and leaf described in embodiment 1 is the herbal medicine of main ingredient, and the parts by weight of described membranous milkvetch stem and leaf are 70, described
The parts by weight of dandelion herb be 20, the parts by weight of described bracteal leaf of corn are 20, the parts by weight of described balsampear stem
Number is 20.
Embodiment 3：
Membranous milkvetch stem and leaf described in embodiment 1 is the herbal medicine of main ingredient, and the parts by weight of described membranous milkvetch stem and leaf are 40, described
The parts by weight of dandelion herb be 10, the parts by weight of described bracteal leaf of corn are 10, the parts by weight of described balsampear stem
Number is 10.
Embodiment 4：
Membranous milkvetch stem and leaf described in embodiment 1 is the herbal medicine of main ingredient, and the parts by weight of described membranous milkvetch stem and leaf are 60, described
The parts by weight of dandelion herb be 15, the parts by weight of described bracteal leaf of corn are 15, the parts by weight of described balsampear stem
Number is 15.
Embodiment 5：
Membranous milkvetch stem and leaf described in embodiment 1 is the herbal medicine of main ingredient, and the weight of described membranous milkvetch stem and leaf is 50g, described
The weight of dandelion herb is 50g, and the weight of described bracteal leaf of corn is 50g, and the weight of described balsampear stem is 50g.
Embodiment 6：
A kind of membranous milkvetch stem and leaf is the preparation method of the herbal medicine of main ingredient, and the first step prepares membranous milkvetch stem and leaf decocting liquid, claimed first
The parts by weight for taking membranous milkvetch stem and leaf are 70 ~ 40, are crushed, and then add 8 times of amount water and soak 20-24 hours, extraction 3 times, often
Secondary 2 h, mixed extract is finally concentrated；
Second step prepares dandelion herb decocting liquid, and the parts by weight that dandelion herb is weighed first are 20 ~ 10, are carried out
Crush, then add 5 times of amount water and soak 20-24 hours, extract 3 times, 2 h, mixed extract, are finally concentrated every time；
3rd step prepares bracteal leaf of corn decocting liquid, and the parts by weight that bracteal leaf of corn is weighed first are 20 ~ 10, are crushed,
Then 10 times of amount water are added to soak 20-24 hours, are extracted 3 times, 2 h, mixed extract, are finally concentrated every time；
4th step prepares balsampear stem decocting liquid, and the parts by weight that balsampear stem is weighed first are 20 ~ 10, are crushed, then
Add 8 times of amount water to soak 20-24 hours, extract 3 times, 2 h, mixed extract, are finally concentrated every time；
5th step is according to membranous milkvetch stem and leaf decocting liquid：Dandelion herb decocting liquid：Bracteal leaf of corn decocting liquid：Balsampear stem decocting liquid
Ratio be 6:2:1:1 ratio mixing, it is membranous milkvetch stem and leaf compound decocting liquid to carry out centrifugal treating and supernatant is made.Membranous milkvetch stem and leaf
Decocting liquid：Dandelion herb decocting liquid：Bracteal leaf of corn decocting liquid：The ratio of balsampear stem decocting liquid is ratio of weight and number.
Embodiment 7：
Membranous milkvetch stem and leaf described in embodiment 6 is the preparation method of the herbal medicine of main ingredient, and the first step prepares membranous milkvetch stem and leaf decocting
Liquid, the weight that membranous milkvetch stem and leaf is weighed first is 50g, is crushed, and then adds 8 times of amount water and soaks 20-24 hours, extracts 3 times,
2 h, mixed extract, are finally concentrated every time；
Second step prepares dandelion herb decocting liquid, and the weight that dandelion herb is weighed first is 50g, is crushed, so
5 times of amount water are added afterwards to soak 20-24 hours, are extracted 3 times, 2 h, mixed extract, are finally concentrated every time；
3rd step prepares bracteal leaf of corn decocting liquid, and the weight that bracteal leaf of corn is weighed first is 50g, is crushed, Ran Houjia
Enter 10 times of amount water to soak 20-24 hours, extract 3 times, 2 h, mixed extract, are finally concentrated every time；
4th step prepares balsampear stem decocting liquid, and balsampear stem weight is weighed first for 50g, is crushed, and then adds 8 times of amounts
Water soaks 20-24 hours, extracts 3 times, 2 h, mixed extract, are finally concentrated every time；
5th step is according to membranous milkvetch stem and leaf decocting liquid：Dandelion herb decocting liquid：Bracteal leaf of corn decocting liquid：Balsampear stem decocting liquid
Ratio be 6:2:1:1 ratio mixing, it is membranous milkvetch stem and leaf compound decocting liquid to carry out centrifugal treating and supernatant is made.
Embodiment 8：
Membranous milkvetch stem and leaf described in embodiment 6 or 7 is the preparation method of the herbal medicine of main ingredient, and described is extracted as decocting cooking method
Extract decocting liquid；Described concentration is the 2/3 of the decocting liquid weight for being concentrated into a procedure.
Embodiment 9：
Membranous milkvetch stem and leaf described in above-described embodiment is the herbal medicine of main ingredient, the membranous milkvetch stem and leaf compound decocting liquid pair of various dose
The influence of ND Vaccine immune effect.
Material
(1.1) medicine
Membranous milkvetch stem and leaf compound decocting liquid
(1.2) experimental animal and raising
The blue brown male chicken 160 in 1 age in days health sea, full price feed for layer chicken, experiment fowl raising is tested in Jiamusi University
Animal center, free choice feeding and drinking-water.
Vaccine
Chicken Newcastle disease live vaccine（Sota plants of La）.
Antibody test reagent
The anti-NDV hemagglutination inhibition antibodies of standard（Serum）, newcastle disease standard HI antigens, phosphate buffer (PBS,
PH7.2) prepare, 1% chicken erythrocyte suspension (RBC) is prepared and the virus liquid of 4 blood coagulation units is prepared by GB/T16550-
2008 are carried out.
（1.5）Key instrument material
Electronic balance, inverted microscope, low speed centrifuge, intelligent steam sterilizer etc..
Method
（2.1）Packet and disposal
The blue brown male chicken in 1 age in days sea, raises to choosing 160 during 7 age in days, is randomly divided into 8 groups, every group 20.Control
Group same volume, synthermal pure water drip, daily 1 time, continuous 7d；Remaining 7 groups, the Radix Astragali is given by multiple proportions graded doses
Cauline leaf compound decocting liquid；Each group feeding and management condition is completely the same such as (table 1) experiment packet and disposal.
（2.2）Antibody titres to newcastle disease virus is determined
When 14,21,28 age in days, each drug dose group and every group of control group are taken out 6 jugular veins and adopted at random
Blood, separates serum, and the dynamic change of antibody titres to newcastle disease virus is determined with Hemagglutination Inhibition.
（2.3）Data statistics
Test data carries out statistical disposition with SPSS19.0 softwares, is as a result represented with average () ± standard deviation (SD), P<
0.05 is significant difference, P<0.01 is that difference is extremely notable.
As a result
2.4.1 the change of antibody titres to newcastle disease virus
The dynamic change of the change such as table 2 below each group antibody titres to newcastle disease virus of extra large blue brown public young each group antibody titres to newcastle disease virus
It is shown.
Conclusion：As shown in Table 2, after newcastle disease vaccine is immune, chick anti-new castle disease virus antibody level is significantly raised, takes orally
4 groups（Per 1kg body weight 0.4g）The membranous milkvetch stem and leaf compound decocting liquid of dosage, and the anti-new city of chick being immunized with newcastle disease vaccine
Epidemic disease poison antibody level is raised than vaccine control group faster maintains the time of higher level longer, compared with 1 group（Per 1kg body weight
0.05g）, 2 groups（Per 1kg body weight 0.10g）, 3 groups（Per 1kg body weight 0.20g）, 5 groups（Per 1kg body weight
0.80g）, 6 groups（Per 1kg body weight 1.60g）Dosage group effect becomes apparent.Therefore, membranous milkvetch stem and leaf compound decocting liquid is new to chicken
City epidemic disease immune effect of vaccine has obvious humidification, and membranous milkvetch stem and leaf compound decocting liquid is comparatively ideal medicament selection.
Embodiment 10：
Membranous milkvetch stem and leaf described in above-described embodiment is the herbal medicine of main ingredient, the membranous milkvetch stem and leaf compound decocting liquid pair of various dose
The In-vitro Inhibitory Effect of newcastle disease virus has very big influence.
Material
（1.1）Medicine
Membranous milkvetch stem and leaf compound decocting liquid
（1.2）Virus and cell
Newcastle disease poison strain (NDV), baby hamster kidney passage cell（BHK-21）, preserve, pass on, train according to a conventional method
Support.
（1.3）Main agents and instrument
Standard neonatal cow's serum (without inactivation), trypsase.Inverted microscope, 371 type CO2Incubator, 96 hole cells
Plate, 800 type centrifuges.
Method
（2.1 ）NDV tissue culture infective dose（TCID50）Determine
NDV virus liquids are serially diluted by 10 times with cell maintenance medium, dilution factor is respectively plus 10-1,10-2,10-3,10-
4、10-5、10-6、10-7.Then by every hole 0.1mL amounts plus people to having grown up on the BHK-21 cells of individual layer, each dilution factor 4
Hole.Normal cell controls are set up simultaneously, 37 DEG C, 5% CO is placed in272h is cultivated in incubator, daily observation cells characteristic lesion
(CPE), TCID is calculated with Reed-Muench formula50。
（2.2）Membranous milkvetch stem and leaf compound decocting liquid is determined to the non-toxic concn of BHK-21 cells
It will be added after BHK-21 cell dissociations in 96 porocyte culture plates, 37 DEG C of people, 5% CO are put in 100 μ L/ holes2Incubator
Middle culture 24h.After cells grow up to the individual layer, sucks the nutrient solution on cell monolayer.Membranous milkvetch stem and leaf compound decocting liquid is maintained
Liquid does doubling dilution, has 11 dilution factors, Chinese medicine diluted concentration is from 200mg/mL until 0.195mg/mL.By decoction plus people
Suck in 96 porocyte culture plates of nutrient solution, add 100 μ L per hole, while setting up normal cell controls in last hole, put 37
℃、5% CO272h is cultivated in incubator, daily observation cells characteristic lesion (CPE).
（2.3 ）Direct killing action of the membranous milkvetch stem and leaf compound decocting liquid to NDV
First with maintaining liquid by NDV TCID50Dilution, then membranous milkvetch stem and leaf compound decocting liquid is carried out in the range of non-toxic concn
Doubling dilution, the medicine of each dilution factor and the virus liquid of equal volume are acted in 37 DEG C of water-baths adds 96 hole cell trainings after 2h
Support on plate, per the μ L of hole 100, while setting up virus control group and cell controls group, put 37 DEG C, 5% CO272h is cultivated in incubator,
The CPE situations of each dilution factor and control group are recorded when the CPE of virus control group reaches 75%-100%.There is more than 75% cell
Lesion is designated as +++, more than 50% lesion is designated as ++, more than 25% lesion is designated as+, no lesion is designated as-.
（2.4）Blocking effect of the membranous milkvetch stem and leaf compound decocting liquid to NDV
Doubling dilution is carried out to membranous milkvetch stem and leaf compound decocting liquid in non-toxic concn boundary with maintaining liquid, length is then added to
Into on the well culture plate of BHK-21 cells 96 of individual layer, 100 μ L/hole, each dilution factor adds 4 holes.37℃、5% CO2Incubator
Middle effect 4h, then adds 1000 TCID50Viral 0.1mL, and set up normal cell controls group and virus control group.Put 37
℃、5% CO2Continue to cultivate in incubator, CPE situations are recorded after 72h, recording method is ibid.
Result and analysis
（3.1）TCID50Determine
After virus inoculation cell 96h, NDV TCID is measured50For 10-6.75/0.1mL.
（3.2）Membranous milkvetch stem and leaf compound decocting liquid is determined to the non-toxic concn of individual layer BHK-21 cells
Selection is used as non-toxic concn of the medicine to BHK-21 cells to Cmaxs of the individual layer BHK-21 without influence.By
Membranous milkvetch stem and leaf compound decocting liquid is 2.48mg/mL to the non-toxic concn of individual layer BHK-21 cells after 72h cultures.
（3.3 ）Direct killing action of the membranous milkvetch stem and leaf compound decocting liquid to NDV
Membranous milkvetch stem and leaf compound decocting liquid is shown in Table 3 membranous milkvetch stem and leaf compound decocting liquids to NDV direct killing action to the straight of NDV
Connect killing action（n=4）
（3.4 ）Blocking effect of the membranous milkvetch stem and leaf compound decocting liquid to NDV
Membranous milkvetch stem and leaf water compound decocting liquid is shown in Table blocking of the 4 membranous milkvetch stem and leaf compound decocting liquids to NDV to NDV blocking effect and made
With
From table 3-4, membranous milkvetch stem and leaf compound decocting liquid has to NDV preferably directly to be killed and blocking effect effect, its
External minimum kills and blocked concentration to be 0.155 mg/mL.Show in NDV treatment and prevention, membranous milkvetch stem and leaf compound decocting
Liquid is comparatively ideal medicament selection.
Embodiment 11：
Membranous milkvetch stem and leaf described in above-described embodiment is the herbal medicine of main ingredient, the membranous milkvetch stem and leaf compound decocting liquid pair of various dose
The influence of the coat quality of beaver rabbit.
Material
（1.1）Medicine
Membranous milkvetch stem and leaf compound decocting liquid
（1.2）Experimental animal and packet
Experiment is divided into 5 groups, every group 40, each repetition 1 at random using the close weaning rex rabbits 200 of body weight.
The daily ration of membranous milkvetch stem and leaf compound decocting liquid of the wherein 4 groups feedings containing various dose, be specially：Membranous milkvetch stem and leaf compound decocting liquid is dense
Degree is 0.1 mg/mL, the 1st group of 5 mL, the 2nd group of 10mL, the 3rd group of 15 mL, the 4th group of 20 mL.Daily ration based on control group.
Feeding management and epidemic prevention
Before experiment, the rabbit home to experiment carries out thorough disinfection, and experiment beaver rabbit single cage is raised, daily sooner or later each feeding one
It is secondary, free choice feeding.Conventinal breeding is managed and immune programme for children, the d of preliminary trial period 7, the positive examination d of the phase 53.Every 3 days in feeding process
Once sterilizing is carried out to rabbit home.
The collection and preparation of sample
The last day tested respectively in 1 phase and 2 phases is sampled, and it is to try to randomly select experiment rabbit and carry out fur collection
Test and fur is taken after rabbit is butchered, shoulder, shoulder blade, the fur at stern position on clip rabbit, stand-by respectively.
Coat quality
Experiment rabbit weighs fur weight after butchering, and measures hide area.Surface is accumulated：Use tape measure（Formula=skin length × skin
It is wide）.Skin length is to root of the tail portion at depressed neck part, and skin is wide wide to the folded dermatotome neatly located with neck and root of the tail.
Interpretation of result for the various dose of table 5 influence of the membranous milkvetch stem and leaf compound decocting liquid to the coat quality of beaver rabbit.
As shown in Table 5, the hide area of daily ration beaver rabbit of the feeding containing membranous milkvetch stem and leaf compound decocting liquid, hide weight are excellent
In control group.The membranous milkvetch stem and leaf compound decocting liquid of various dose has a significant impact to beaver rabbit hide area, hide weight simultaneously,
The area and weight of hide have increased trend with the raising of membranous milkvetch stem and leaf compound decocting liquid consumption, illustrate that membranous milkvetch stem and leaf is answered
Growth of the square decocting liquid to beaver rabbit fur has certain facilitation.Therefore, membranous milkvetch stem and leaf compound decocting liquid is comparatively ideal
Medicament selection.
Embodiment 12：
A kind of membranous milkvetch stem and leaf is the preparation method of the herbal medicine of main ingredient, and the first step prepares membranous milkvetch stem and leaf decocting liquid, claimed first
The parts by weight for taking membranous milkvetch stem and leaf are 70, are crushed, and then add 8 times of amount water and soak 20 hours, extraction 3 times, every time 2 h,
Mixed extract, is finally concentrated；
Second step prepares dandelion herb decocting liquid, and the parts by weight that dandelion herb is weighed first are 20, are crushed,
Then 5 times of amount water are added to soak 20 hours, are extracted 3 times, 2 h, mixed extract, are finally concentrated every time；
3rd step prepares bracteal leaf of corn decocting liquid, and the parts by weight that bracteal leaf of corn is weighed first are 20, are crushed, then
Add 10 times of amount water to soak 20 hours, extract 3 times, 2 h, mixed extract, are finally concentrated every time；
4th step prepares balsampear stem decocting liquid, and the parts by weight that balsampear stem is weighed first are 20, are crushed, then added
8 times of amount water soak 20 hours, extract 3 times, 2 h, mixed extract, are finally concentrated every time；
5th step is according to membranous milkvetch stem and leaf decocting liquid：Dandelion herb decocting liquid：Bracteal leaf of corn decocting liquid：Balsampear stem decocting liquid
Ratio be 6:2:1:1 ratio mixing, it is membranous milkvetch stem and leaf compound decocting liquid to carry out centrifugal treating and supernatant is made.Membranous milkvetch stem and leaf
Decocting liquid：Dandelion herb decocting liquid：Bracteal leaf of corn decocting liquid：The ratio of balsampear stem decocting liquid is ratio of weight and number.
Embodiment 13：
A kind of membranous milkvetch stem and leaf is the preparation method of the herbal medicine of main ingredient, and the first step prepares membranous milkvetch stem and leaf decocting liquid, claimed first
The parts by weight for taking membranous milkvetch stem and leaf are 40, are crushed, and then add 8 times of amount water and soak 24 hours, extraction 3 times, every time 2 h,
Mixed extract, is finally concentrated；
Second step prepares dandelion herb decocting liquid, and the parts by weight that dandelion herb is weighed first are 10, carry out powder
It is broken, then add 5 times of amount water and soak 24 hours, extract 3 times, 2 h, mixed extract, are finally concentrated every time；
3rd step prepares bracteal leaf of corn decocting liquid, and the parts by weight that bracteal leaf of corn is weighed first are 10, are crushed, so
10 times of amount water are added afterwards to soak 24 hours, are extracted 3 times, 2 h, mixed extract, are finally concentrated every time；
4th step prepares balsampear stem decocting liquid, and the parts by weight that balsampear stem is weighed first are 10, are crushed, Ran Houjia
Enter 8 times of amount water to soak 24 hours, extract 3 times, 2 h, mixed extract, are finally concentrated every time；
5th step is according to membranous milkvetch stem and leaf decocting liquid：Dandelion herb decocting liquid：Bracteal leaf of corn decocting liquid：Balsampear stem decocting liquid
Ratio be 6:2:1:1 ratio mixing, it is membranous milkvetch stem and leaf compound decocting liquid to carry out centrifugal treating and supernatant is made.
Embodiment 14：
A kind of membranous milkvetch stem and leaf is the preparation method of the herbal medicine of main ingredient, and the first step prepares membranous milkvetch stem and leaf decocting liquid, claimed first
The parts by weight for taking membranous milkvetch stem and leaf are 50, are crushed, and then add 8 times of amount water and soak 22 hours, extraction 3 times, every time 2 h,
Mixed extract, is finally concentrated；
Second step prepares dandelion herb decocting liquid, and the parts by weight that dandelion herb is weighed first are 15, carry out powder
It is broken, then add 5 times of amount water and soak 22 hours, extract 3 times, 2 h, mixed extract, are finally concentrated every time；
3rd step prepares bracteal leaf of corn decocting liquid, and the parts by weight that bracteal leaf of corn is weighed first are 15, are crushed, then
Add 10 times of amount water to soak 22 hours, extract 3 times, 2 h, mixed extract, are finally concentrated every time；
4th step prepares balsampear stem decocting liquid, and the parts by weight that balsampear stem is weighed first are 15, are crushed, then added
8 times of amount water soak 22 hours, extract 3 times, 2 h, mixed extract, are finally concentrated every time；
5th step is according to membranous milkvetch stem and leaf decocting liquid：Dandelion herb decocting liquid：Bracteal leaf of corn decocting liquid：Balsampear stem decocting liquid
Ratio be 6:2:1:1 ratio mixing, it is membranous milkvetch stem and leaf compound decocting liquid to carry out centrifugal treating and supernatant is made.
Although above having carried out detailed elaboration to the present invention with general explanation and embodiment,
On the basis of the present invention, some modifications can be made to it or are improved.Therefore, institute without departing from theon the basis of the spirit of the present invention
The modification made adds improvement, belongs to the scope of protection of present invention.
Claims (4)
1. a kind of membranous milkvetch stem and leaf is the herbal medicine of main ingredient, it is made up of following raw material:Membranous milkvetch stem and leaf, dandelion herb, corn bud
Leaf, balsampear stem, it is characterized in that:The parts by weight of described membranous milkvetch stem and leaf are 70 ~ 40, the parts by weight of described dandelion herb
Number is 20 ~ 10, and the parts by weight of described bracteal leaf of corn are 20 ~ 10, and the parts by weight of described balsampear stem are 20 ~ 10.
2. membranous milkvetch stem and leaf according to claim 1 is the herbal medicine of main ingredient, it is characterized in that:The weight of described membranous milkvetch stem and leaf
It is 60 to measure number, and the parts by weight of described dandelion herb are 15, and the parts by weight of described bracteal leaf of corn are 15, described
Balsampear stem parts by weight be 15.
3. a kind of membranous milkvetch stem and leaf is the preparation method of the herbal medicine of main ingredient, it is characterized in that:The first step prepares membranous milkvetch stem and leaf decocting
Liquid, the parts by weight that membranous milkvetch stem and leaf is weighed first are 70 ~ 40, are crushed, and then add 8 times of amount water and soak 20-24 hours,
Extract 3 times, 2 h, mixed extract, are finally concentrated every time；
Second step prepares dandelion herb decocting liquid, and the parts by weight that dandelion herb is weighed first are 20 ~ 10, are crushed,
Then 5 times of amount water are added to soak 20-24 hours, are extracted 3 times, 2 h, mixed extract, are finally concentrated every time；
3rd step prepares bracteal leaf of corn decocting liquid, and the parts by weight that bracteal leaf of corn is weighed first are 20 ~ 10, are crushed, then
Add 10 times of amount water to soak 20-24 hours, extract 3 times, 2 h, mixed extract, are finally concentrated every time；
4th step prepares balsampear stem decocting liquid, and the parts by weight that balsampear stem is weighed first are 20 ~ 10, are crushed, then added
8 times of amount water soak 20-24 hours, extract 3 times, 2 h, mixed extract, are finally concentrated every time；
5th step is according to membranous milkvetch stem and leaf decocting liquid：Dandelion herb decocting liquid：Bracteal leaf of corn decocting liquid：The ratio of balsampear stem decocting liquid
Example is 6:2:1:1 ratio mixing, it is membranous milkvetch stem and leaf compound decocting liquid to carry out centrifugal treating and supernatant is made.
4. membranous milkvetch stem and leaf according to claim 3 is the preparation method of the herbal medicine of main ingredient, it is characterized in that:Described carries
It is taken as decocting cooking method and extracts decocting liquid；Described concentration is the 2/3 of the decocting liquid weight for being concentrated into a procedure.
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| 玉米苞叶对5月龄獭兔营养物质消化及生产性能的影响;李晟等;《饲料广角》;20101231(第14期);第37-38页，尤其是第37页左侧第15行，第38页右侧第2-3行 * |
| 蒲公英、北五味子水煎剂对鸡新城疫疫苗免疫效果的影响;杨立萍等;《广东畜牧兽医科技》;20140418;第39卷(第2期);第22-24页，尤其是第24页左侧第1-2段 * |
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