CN104352826A - All-natural green traditional Chinese veterinary drug taking astragalus membranaceus stems and leaves as main drug as well as preparation method - Google Patents
All-natural green traditional Chinese veterinary drug taking astragalus membranaceus stems and leaves as main drug as well as preparation method Download PDFInfo
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- CN104352826A CN104352826A CN201410530757.1A CN201410530757A CN104352826A CN 104352826 A CN104352826 A CN 104352826A CN 201410530757 A CN201410530757 A CN 201410530757A CN 104352826 A CN104352826 A CN 104352826A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/481—Astragalus (milkvetch)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/288—Taraxacum (dandelion)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/42—Cucurbitaceae (Cucumber family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0087—Galenical forms not covered by A61K9/02 - A61K9/7023
- A61K9/0095—Drinks; Beverages; Syrups; Compositions for reconstitution thereof, e.g. powders or tablets to be dispersed in a glass of water; Veterinary drenches
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Abstract
The invention discloses an all-natural green traditional Chinese veterinary drug taking astragalus membranaceus stems and leaves as a main drug as well as a preparation method. The all-natural green traditional Chinese veterinary drug taking the astragalus membranaceus stems and leaves as the main drug comprises components in parts by weight as follows: 70-40 parts of astragalus membranaceus stems and leaves, 20-10 parts of dandelion whole herbs, 20-10 parts of corn bracts and 20-10 parts of balsam pear stems.
Description
Technical field
the present invention relates to fully natural green herbal medicine and manufacture method that a kind of stem and leaf of Radix Astragali is principal agent.
Background technology
stem and leaf of Radix Astragali beginning of being used as medicine is loaded in " Mingyi Bielu ", its sweet in the mouth, pungent, property is put down, enter the heart, liver two warp, there is promoting the production of body fluid to quench thirst, relaxing muscles and tendons to promote blood circulation, detumescence treat the function such as skin ulcer, cure mainly quench one's thirst, the disease such as thirsty and dry pharynx, the contracture of muscle arteries and veins, joint joint stuffiness, carbuncle furuncle of consumption of YIN caused by febrile disease.Herba Taraxaci herb has antiinflammatory, antioxidation, anticancer, the effect such as hyperglycemia, antithrombus formation.Bracteal leaf of corn has reduction arteriosclerosis, reduces hyperlipidemia, also has good Scavenging activity to hydroxyl radical free radical and super radical anion.Caulis Momordicae Charantiae has effects such as improving body immunity, antitumor, antiviral.These all illustrate with stem and leaf of Radix Astragali to be that the compound pure natural green beasts Chinese medicine that major ingredient is equipped with Herba Taraxaci herb, bracteal leaf of corn, Caulis Momordicae Charantiae are made has good exploitation prospect.
Summary of the invention
the object of this invention is to provide fully natural green herbal medicine and manufacture method that a kind of immunity, stem and leaf of Radix Astragali of accelerating growth rate that can improve beasts is principal agent.
above-mentioned object is realized by following technical scheme:
a kind of stem and leaf of Radix Astragali is the fully natural green herbal medicine of principal agent, its composition comprises: stem and leaf of Radix Astragali, Herba Taraxaci herb, bracteal leaf of corn, Caulis Momordicae Charantiae, the parts by weight of described stem and leaf of Radix Astragali are 70 ~ 40, the parts by weight of described Herba Taraxaci herb are 20 ~ 10, the parts by weight of described bracteal leaf of corn are 20 ~ 10, and the parts by weight of described Caulis Momordicae Charantiae are 20 ~ 10.
described stem and leaf of Radix Astragali is the fully natural green herbal medicine of principal agent, and the parts by weight of described stem and leaf of Radix Astragali are 60, and the parts by weight of described Herba Taraxaci herb are 15, and the parts by weight of described bracteal leaf of corn are 15, and the parts by weight of described Caulis Momordicae Charantiae are 15.
a kind of stem and leaf of Radix Astragali is the manufacture method of the fully natural green herbal medicine of principal agent, the first step prepares stem and leaf of Radix Astragali decocting liquid, first the parts by weight taking stem and leaf of Radix Astragali are 70 ~ 40, pulverize, then add 8 times amount water soaking 20-24 hour, extract 3 times, each 2 h, mixed extract, finally concentrates;
second step prepares Herba Taraxaci herb decocting liquid, and the parts by weight first taking Herba Taraxaci herb are 20 ~ 10, pulverize, and then adds 5 times amount water soaking 20-24 hour, and extract 3 times, each 2 h, mixed extract, finally concentrates;
3rd step prepares bracteal leaf of corn decocting liquid, and the parts by weight first taking bracteal leaf of corn are 20 ~ 10, pulverize, and then adds 10 times amount water soaking 20-24 hour, and extract 3 times, each 2 h, mixed extract, finally concentrates;
4th step prepares Caulis Momordicae Charantiae decocting liquid, and the parts by weight first taking Caulis Momordicae Charantiae are 20 ~ 10, pulverize, and then adds 8 times amount water soaking 20-24 hour, and extract 3 times, each 2 h, mixed extract, finally concentrates;
5th step is according to stem and leaf of Radix Astragali decocting liquid: Herba Taraxaci herb decocting liquid: bracteal leaf of corn decocting liquid: the ratio of Caulis Momordicae Charantiae decocting liquid is the ratio mixing of 6:2:1:1, and carrying out centrifugal treating, to obtain supernatant be stem and leaf of Radix Astragali compound recipe decocting liquid.
described stem and leaf of Radix Astragali is the manufacture method of the fully natural green herbal medicine of principal agent, the first step prepares stem and leaf of Radix Astragali decocting liquid, first the weight taking stem and leaf of Radix Astragali is 50g, pulverize, then add 8 times amount water soaking 20-24 hour, extract 3 times, each 2 h, mixed extract, finally concentrates;
second step prepares Herba Taraxaci herb decocting liquid, and the weight first taking Herba Taraxaci herb is 50g, pulverizes, and then adds 5 times amount water soaking 20-24 hour, and extract 3 times, each 2 h, mixed extract, finally concentrates;
3rd step prepares bracteal leaf of corn decocting liquid, and the weight first taking bracteal leaf of corn is 50g, pulverizes, and then adds 10 times amount water soaking 20-24 hour, and extract 3 times, each 2 h, mixed extract, finally concentrates;
4th step prepares Caulis Momordicae Charantiae decocting liquid, and first taking Caulis Momordicae Charantiae weight is 50g, pulverizes, and then adds 8 times amount water soaking 20-24 hour, and extract 3 times, each 2 h, mixed extract, finally concentrates;
5th step is according to stem and leaf of Radix Astragali decocting liquid: Herba Taraxaci herb decocting liquid: bracteal leaf of corn decocting liquid: the ratio of Caulis Momordicae Charantiae decocting liquid is the ratio mixing of 6:2:1:1, and carrying out centrifugal treating, to obtain supernatant be stem and leaf of Radix Astragali compound recipe decocting liquid.
described stem and leaf of Radix Astragali is the manufacture method of the fully natural green herbal medicine of principal agent, and the described decocting cooking method that is extracted as extracts decocting liquid; Described simmer down to is concentrated into 2/3 of the decocting liquid weight of one procedure.
beneficial effect:
1. the present invention adopts stem and leaf of Radix Astragali compound recipe decocting liquid, significantly can strengthen immune effect of vaccine, promotes that Immune Organs of Chicken is grown, and has newcastle disease virus (NDV) and directly kill and blocking effect.
the present invention adopts stem and leaf of Radix Astragali compound recipe decocting liquid, significantly can alleviate the cytopathy that virus causes, and have certain dose-effect relationship.
the present invention adopts stem and leaf of Radix Astragali compound recipe decocting liquid, can significantly improve the coat quality of rex rabbit, mink.
the present invention has promotion chicken non-specific immunity, ND Vaccine is coordinated to promote specific immune function, by observing the index such as antibody titer change, lymphopoiesis situation, erythrocyte yeast rosette rate, Immune Organs Index, growth performance in doses interval.
the present invention adopts the In-vitro Inhibitory Effect of stem and leaf of Radix Astragali compound recipe decocting liquid to newcastle disease virus to have great raising.
the present invention all has appreciable impact to rex rabbit, mink hide area, hide weight, the area of hide and weight all have the trend of increase along with the raising of stem and leaf of Radix Astragali compound recipe decocting liquid consumption, illustrate that the growth of stem and leaf of Radix Astragali compound recipe decocting liquid to rex rabbit, mink fur has certain facilitation.
the present invention take stem and leaf of Radix Astragali as the compound pure natural green herbal medicine that major ingredient is equipped with Herba Taraxaci herb, bracteal leaf of corn, Caulis Momordicae Charantiae are made, and use safety, has no side effect.
the present invention with stem and leaf of Radix Astragali be major ingredient is equipped with Herba Taraxaci herb, bracteal leaf of corn, Caulis Momordicae Charantiae are made compound pure natural green herbal medicine develop can make all dosage forms such as oral liquid, injection, tablet, capsule, injectable powder, pill, powder, easy to use, effective.
the present invention is the various active component extracted from stem and leaf of Radix Astragali is major ingredient, and the various active component extracted in Herba Taraxaci herb, bracteal leaf of corn, Caulis Momordicae Charantiae are the herbal medicine that adjuvant develops, successful.
the present invention is principal agent with stem and leaf of Radix Astragali, is equipped with Herba Taraxaci herb, bracteal leaf of corn, hardship
the fully natural green compound traditional Chinese veterinary medicine that melon vine is made; Radix Astragali tradition agents area is root, and when it is used as medicine, a large amount of stem and leaf is abandoned it, containing plurality of active ingredients such as saponin, polysaccharide, aminoacid, protein, flavone in stem and leaf of Radix Astragali, has the effects such as raising is immune, antibacterial, antiviral, antioxidation.Herba Taraxaci, bracteal leaf of corn, Caulis Momordicae Charantiae have antiinflammatory equally, improve the effects such as body immunity, antitumor, antiviral.
the present invention take stem and leaf of Radix Astragali as principal agent; Herba Taraxaci herb, bracteal leaf of corn, Caulis Momordicae Charantiae are adjuvant; this compound preparation is intended to be used for field of traditional Chinese veterinary; not only effectively protect Radix Astragali resource; achieve the sustainable use of the Radix Astragali; in addition the coordination pharmacological action of Herba Taraxaci herb, bracteal leaf of corn, Caulis Momordicae Charantiae; more effectively enhance the pharmacologically actives such as the immunologic function of the animals such as birds, antiviral, antibacterial, antiinflammatory; show that this compound preparation can also improve fleece animal pelage quality after deliberation, strengthen the body weight of animal.
detailed description of the invention:
embodiment 1:
a kind of stem and leaf of Radix Astragali is the fully natural green herbal medicine of principal agent, its composition comprises: stem and leaf of Radix Astragali, Herba Taraxaci herb, bracteal leaf of corn, Caulis Momordicae Charantiae, the parts by weight of described stem and leaf of Radix Astragali are 70 ~ 40, the parts by weight of described Herba Taraxaci herb are 20 ~ 10, the parts by weight of described bracteal leaf of corn are 20 ~ 10, and the parts by weight of described Caulis Momordicae Charantiae are 20 ~ 10.
embodiment 2:
stem and leaf of Radix Astragali described in embodiment 1 is the fully natural green herbal medicine of principal agent, the parts by weight of described stem and leaf of Radix Astragali are 70, the parts by weight of described Herba Taraxaci herb are 20, and the parts by weight of described bracteal leaf of corn are 20, and the parts by weight of described Caulis Momordicae Charantiae are 20.
embodiment 3:
stem and leaf of Radix Astragali described in embodiment 1 is the fully natural green herbal medicine of principal agent, the parts by weight of described stem and leaf of Radix Astragali are 40, the parts by weight of described Herba Taraxaci herb are 10, and the parts by weight of described bracteal leaf of corn are 10, and the parts by weight of described Caulis Momordicae Charantiae are 10.
embodiment 4:
stem and leaf of Radix Astragali described in embodiment 1 is the fully natural green herbal medicine of principal agent, the parts by weight of described stem and leaf of Radix Astragali are 60, the parts by weight of described Herba Taraxaci herb are 15, and the parts by weight of described bracteal leaf of corn are 15, and the parts by weight of described Caulis Momordicae Charantiae are 15.
embodiment 5:
stem and leaf of Radix Astragali described in embodiment 1 is the fully natural green herbal medicine of principal agent, and the weight of described stem and leaf of Radix Astragali is 50g, and the weight of described Herba Taraxaci herb is 50g, and the weight of described bracteal leaf of corn is 50g, and the weight of described Caulis Momordicae Charantiae is 50g.
embodiment 6:
a kind of stem and leaf of Radix Astragali is the manufacture method of the fully natural green herbal medicine of principal agent, the first step prepares stem and leaf of Radix Astragali decocting liquid, first the parts by weight taking stem and leaf of Radix Astragali are 70 ~ 40, pulverize, then add 8 times amount water soaking 20-24 hour, extract 3 times, each 2 h, mixed extract, finally concentrates;
second step prepares Herba Taraxaci herb decocting liquid, and the parts by weight first taking Herba Taraxaci herb are 20 ~ 10, pulverize, and then adds 5 times amount water soaking 20-24 hour, and extract 3 times, each 2 h, mixed extract, finally concentrates;
3rd step prepares bracteal leaf of corn decocting liquid, and the parts by weight first taking bracteal leaf of corn are 20 ~ 10, pulverize, and then adds 10 times amount water soaking 20-24 hour, and extract 3 times, each 2 h, mixed extract, finally concentrates;
4th step prepares Caulis Momordicae Charantiae decocting liquid, and the parts by weight first taking Caulis Momordicae Charantiae are 20 ~ 10, pulverize, and then adds 8 times amount water soaking 20-24 hour, and extract 3 times, each 2 h, mixed extract, finally concentrates;
5th step is according to stem and leaf of Radix Astragali decocting liquid: Herba Taraxaci herb decocting liquid: bracteal leaf of corn decocting liquid: the ratio of Caulis Momordicae Charantiae decocting liquid is the ratio mixing of 6:2:1:1, and carrying out centrifugal treating, to obtain supernatant be stem and leaf of Radix Astragali compound recipe decocting liquid.Stem and leaf of Radix Astragali decocting liquid: Herba Taraxaci herb decocting liquid: bracteal leaf of corn decocting liquid: the ratio of Caulis Momordicae Charantiae decocting liquid is ratio of weight and number.
embodiment 7:
stem and leaf of Radix Astragali described in embodiment 6 is the manufacture method of the fully natural green herbal medicine of principal agent, the first step prepares stem and leaf of Radix Astragali decocting liquid, first the weight taking stem and leaf of Radix Astragali is 50g, pulverize, then add 8 times amount water soaking 20-24 hour, extract 3 times, each 2 h, mixed extract, finally concentrates;
second step prepares Herba Taraxaci herb decocting liquid, and the weight first taking Herba Taraxaci herb is 50g, pulverizes, and then adds 5 times amount water soaking 20-24 hour, and extract 3 times, each 2 h, mixed extract, finally concentrates;
3rd step prepares bracteal leaf of corn decocting liquid, and the weight first taking bracteal leaf of corn is 50g, pulverizes, and then adds 10 times amount water soaking 20-24 hour, and extract 3 times, each 2 h, mixed extract, finally concentrates;
4th step prepares Caulis Momordicae Charantiae decocting liquid, and first taking Caulis Momordicae Charantiae weight is 50g, pulverizes, and then adds 8 times amount water soaking 20-24 hour, and extract 3 times, each 2 h, mixed extract, finally concentrates;
5th step is according to stem and leaf of Radix Astragali decocting liquid: Herba Taraxaci herb decocting liquid: bracteal leaf of corn decocting liquid: the ratio of Caulis Momordicae Charantiae decocting liquid is the ratio mixing of 6:2:1:1, and carrying out centrifugal treating, to obtain supernatant be stem and leaf of Radix Astragali compound recipe decocting liquid.
embodiment 8:
stem and leaf of Radix Astragali described in embodiment 6 or 7 is the manufacture method of the fully natural green herbal medicine of principal agent, and the described decocting cooking method that is extracted as extracts decocting liquid; Described simmer down to is concentrated into 2/3 of the decocting liquid weight of one procedure.
embodiment 9:
stem and leaf of Radix Astragali described in above-described embodiment is the fully natural green herbal medicine of principal agent, and the stem and leaf of Radix Astragali compound recipe decocting liquid of various dose is on the impact of ND Vaccine immune effect.
material
(1.1) medicine
stem and leaf of Radix Astragali compound recipe decocting liquid
(1.2) laboratory animal and raising
the blue brown male chicken 160 in the healthy sea of 1 age in days, full price feed for layer chicken, test chicken is raised in Jiamusi University's Experimental Animal Center, free choice feeding and drinking-water.
vaccine
chicken Newcastle disease live vaccine (La Sota strain).
antibody test reagent
the anti-NDV hemagglutination inhibition antibody (serum) of standard, newcastle disease standard HI antigen, prepared by phosphate buffer (PBS, pH7.2) preparation, 1% chicken erythrocyte suspension (RBC) and the virus liquid preparation of 4 HAUs is undertaken by GB/T16550-2008.
(1.5) key instrument material
electronic balance, inverted microscope, low speed centrifuge, intelligent steam autoclave etc.
. method
(2.1) grouping and disposal
the 1 blue brown male chicken in age in days sea, raising to choosing 160 during 7 age in days, being divided into 8 groups at random, often organize 20.Matched group same volume, synthermal pure water drip, every day 1 time, continuous 7d; All the other 7 groups, give stem and leaf of Radix Astragali compound recipe decocting liquid by multiple proportions graded doses; Each group of feeding and management condition is completely the same as (table 1) test grouping and disposal.
(2.2) antibody titres to newcastle disease virus measures
respectively at 14,21,28 age in days time, each drug dose group and matched group are often organized and are taken out 6 jugular vein blood collection at random, separation of serum, measure the dynamic change of antibody titres to newcastle disease virus with Hemagglutination Inhibition.
(2.3) data statistics
test data carries out statistical disposition with SPSS19.0 software, result with average (
) ± standard deviation (SD) represents, P<0.05 is significant difference, and P<0.01 is that difference is extremely remarkable.
result
2.4.1 the change of antibody titres to newcastle disease virus
change such as the following table 2 of the young each group antibody titres to newcastle disease virus of the blue brown public affairs in sea is respectively organized shown in the dynamic change of antibody titres to newcastle disease virus.
conclusion: as shown in Table 2, after newcastle disease vaccine immunity, chickling anti-new castle disease virus antibody horizontal obviously raises, the stem and leaf of Radix Astragali compound recipe decocting liquid of 4 groups of (every 1kg body weight 0.4g) dosage for oral administration, and raise faster with the chickling anti-new castle disease virus antibody horizontal of newcastle disease vaccine immunity than vaccine matched group, the time maintaining higher level is longer, comparatively 1 group (every 1kg body weight 0.05g), 2 groups (every 1kg body weight 0.10g), 3 groups (every 1kg body weight 0.20g), 5 groups (every 1kg body weight 0.80g), 6 groups of (every 1kg body weight 1.60g) dosage group effects are more obvious.Therefore, stem and leaf of Radix Astragali compound recipe decocting liquid has obvious potentiation to ND Vaccine immune effect, and stem and leaf of Radix Astragali compound recipe decocting liquid is comparatively ideal medicament selection.
embodiment 10:
stem and leaf of Radix Astragali described in above-described embodiment is the fully natural green herbal medicine of principal agent, and the In-vitro Inhibitory Effect of stem and leaf of Radix Astragali compound recipe decocting liquid on newcastle disease virus of various dose has very large impact.
. material
(1.1) medicine
stem and leaf of Radix Astragali compound recipe decocting liquid
(1.2) virus and cell
newcastle disease poison strain (NDV), young hamster kidney passage cell (BHK-21), preserve, go down to posterity, cultivate according to a conventional method.
(1.3) main agents and instrument
standard neonatal Ox blood serum (without deactivation), trypsin.Inverted microscope, 371 type CO
2
incubator, 96 porocyte plates, 800 type centrifuges.
. method
(2.1) tissue culture infective dose (TCID of NDV
50
) measure
with cell maintenance medium by 10 times of serial dilution NDV virus liquids, dilution factor is respectively and adds 10-1,10-2,10-3,10-4,10-5,10-6,10-7.Then people is added on the BHK-21 cell growing up to monolayer, each dilution factor 4 hole by every hole 0.1mL amount.Set up normal cell controls simultaneously, be placed in 37 DEG C, 5% CO
2
cultivate 72h in incubator, observe cells characteristic pathological changes (CPE) every day, with Reed-Muench formulae discovery TCID
50
.
(2.2) stem and leaf of Radix Astragali compound recipe decocting liquid measures the non-toxic concn of BHK-21 cell
to add on 96 porocyte culture plates after BHK-21 cell dissociation, 100 μ L/ holes, put people 37 DEG C, 5% CO
2
24h is cultivated in incubator.Grow up to after monolayer until cell, suck the culture fluid on cell monolayer.Stem and leaf of Radix Astragali compound recipe decocting liquid maintenance medium is done doubling dilution, has 11 dilution factors, Chinese medicine diluted concentration is from 200mg/mL until 0.195mg/mL.Medicinal liquid being added people sucks on 96 porocyte culture plates of culture fluid, and every hole adds 100 μ L, and in the end normal cell controls is set up in a hole simultaneously, puts 37 DEG C, 5% CO
2
cultivate 72h in incubator, observe cells characteristic pathological changes (CPE) every day.
(2.3) stem and leaf of Radix Astragali compound recipe decocting liquid is to the direct killing action of NDV
first with the TCID of maintenance medium by NDV
50
dilution, again within the scope of non-toxic concn, doubling dilution is carried out to stem and leaf of Radix Astragali compound recipe decocting liquid, add on 96 porocyte culture plates after the virus liquid of each dilution medicine and equal volume acts on 2h in 37 DEG C of water-baths, every hole 100 μ L, set up virus control group and cell controls group simultaneously, put 37 DEG C, 5% CO
2
cultivate 72h in incubator, record the CPE situation of each dilution factor and matched group when the CPE of virus control group reaches 75%-100%.Occur that more than 75% cytopathy is designated as +++, more than 50% pathological changes is designated as ++, more than 25% pathological changes is designated as+, without pathological changes be designated as-.
(2.4) stem and leaf of Radix Astragali compound recipe decocting liquid is to the blocking effect of NDV
in non-toxic concn boundary, carry out doubling dilution by maintenance medium to stem and leaf of Radix Astragali compound recipe decocting liquid, then join and grow up on BHK-21 cell 96 well culture plate of monolayer, 100 μ L/holes, each dilution factor adds 4 holes.37 DEG C, 5% CO
2
act on 4h in incubator, then add 1000 TCID
50
viral 0.1mL, and set up normal cell controls group and virus control group.Put 37 DEG C, 5% CO
2
continue in incubator to cultivate, record CPE situation after 72h, recording method is the same.
. results and analysis
(3.1) TCID
50
measure
after virus inoculation cell 96h, record the TCID of NDV
50
for 10-6.75/0.1mL.
(3.2) non-toxic concn of stem and leaf of Radix Astragali compound recipe decocting liquid to monolayer BHK-21 cell is determined
select on monolayer BHK-21 without impact Cmax as the non-toxic concn of this medicine to BHK-21 cell.After 72h cultivates, the non-toxic concn of stem and leaf of Radix Astragali compound recipe decocting liquid to monolayer BHK-21 cell is 2.48mg/mL.
(3.3) stem and leaf of Radix Astragali compound recipe decocting liquid is to the direct killing action of NDV
stem and leaf of Radix Astragali compound recipe decocting liquid to the direct killing action of NDV in table 3 stem and leaf of Radix Astragali compound recipe decocting liquid to the direct killing action (n=4) of NDV
(3.4) stem and leaf of Radix Astragali compound recipe decocting liquid is to the blocking effect of NDV
stem and leaf of Radix Astragali water compound recipe decocting liquid to the blocking effect of NDV in table 4 stem and leaf of Radix Astragali compound recipe decocting liquid to the blocking effect of NDV
from table 3-4, stem and leaf of Radix Astragali compound recipe decocting liquid has NDV directly to be killed and blocking effect effect preferably, and it is external, and minimum to kill and block concentration be 0.155 mg/mL.Show that stem and leaf of Radix Astragali compound recipe decocting liquid is comparatively ideal medicament selection in the treatment and prevention of NDV.
embodiment 11:
stem and leaf of Radix Astragali described in above-described embodiment is the fully natural green herbal medicine of principal agent, and the stem and leaf of Radix Astragali compound recipe decocting liquid of various dose is on the impact of the coat quality of rex rabbit.
. material
(1.1) medicine
stem and leaf of Radix Astragali compound recipe decocting liquid
(1.2) experimental animal and grouping
test adopts the weaning rex rabbits 200 that body weight is close, is divided into 5 groups at random, often organizes 40, each repetition 1.Wherein 4 groups of daily rations of the stem and leaf of Radix Astragali compound recipe decocting liquid containing various dose of feeding, are specially: stem and leaf of Radix Astragali compound recipe decocting liquid concentration is 0.1 mg/mL, the 1st group of 5 mL, the 2nd group of 10mL, the 3rd group of 15 mL, the 4th group of 20 mL.Daily ration based on matched group.
. feeding and management and epidemic prevention
before test, carry out thorough disinfection to the rabbit home of test, test rex rabbit list cage is raised, and every day respectively feeds once sooner or later, free choice feeding.Conventional feeding and management and immune programme for children, preliminary trial period 7 d, is just trying phase 53 d.In feeding process, every 3 days, once sterilizing is carried out to rabbit home.
. the collection of sample and preparation
sample in the last day of 1 phase and the test of 2 phases respectively, it is get fur after being butchered by test rabbit that random selecting test rabbit carries out fur collection, and on difference clip rabbit, the fur at shoulder, shoulder blade, buttocks position, stand-by.
. coat quality
test rabbit butchers rear weighing fur weight, measures hide area.Surface amasss: with tape measure (formula=skin length × skin is wide).Skin length is from depressed neck part to root of the tail portion, and skin is wide wide to the folded dermatotome neatly located with cervical region and root of the tail.
. interpretation of result is that the stem and leaf of Radix Astragali compound recipe decocting liquid of table 5 various dose is on the impact of the coat quality of rex rabbit.
as shown in Table 5, the hide area of the daily ration rex rabbit containing stem and leaf of Radix Astragali compound recipe decocting liquid, the hide weight of feeding all is better than matched group.The stem and leaf of Radix Astragali compound recipe decocting liquid of various dose all has appreciable impact to rex rabbit hide area, hide weight simultaneously, the area of hide and weight all have the trend of increase along with the raising of stem and leaf of Radix Astragali compound recipe decocting liquid consumption, illustrate that the growth of stem and leaf of Radix Astragali compound recipe decocting liquid to rex rabbit fur has certain facilitation.Therefore, stem and leaf of Radix Astragali compound recipe decocting liquid is comparatively ideal medicament selection.
embodiment 12:
stem and leaf of Radix Astragali is a manufacture method for the fully natural green herbal medicine of principal agent, and the first step prepares stem and leaf of Radix Astragali decocting liquid, and the parts by weight first taking stem and leaf of Radix Astragali are 70, pulverize, then add 8 times amount water soaking 20 hours, extract 3 times, each 2 h, mixed extract, finally concentrates;
second step prepares Herba Taraxaci herb decocting liquid, and the parts by weight first taking Herba Taraxaci herb are 20, pulverize, and then adds 5 times amount water soaking 20 hours, and extract 3 times, each 2 h, mixed extract, finally concentrates;
3rd step prepares bracteal leaf of corn decocting liquid, and the parts by weight first taking bracteal leaf of corn are 20, pulverize, and then adds 10 times amount water soaking 20 hours, and extract 3 times, each 2 h, mixed extract, finally concentrates;
4th step prepares Caulis Momordicae Charantiae decocting liquid, and the parts by weight first taking Caulis Momordicae Charantiae are 20, pulverize, and then adds 8 times amount water soaking 20 hours, and extract 3 times, each 2 h, mixed extract, finally concentrates;
5th step is according to stem and leaf of Radix Astragali decocting liquid: Herba Taraxaci herb decocting liquid: bracteal leaf of corn decocting liquid: the ratio of Caulis Momordicae Charantiae decocting liquid is the ratio mixing of 6:2:1:1, and carrying out centrifugal treating, to obtain supernatant be stem and leaf of Radix Astragali compound recipe decocting liquid.Stem and leaf of Radix Astragali decocting liquid: Herba Taraxaci herb decocting liquid: bracteal leaf of corn decocting liquid: the ratio of Caulis Momordicae Charantiae decocting liquid is ratio of weight and number.
embodiment 13:
a kind of stem and leaf of Radix Astragali is the manufacture method of the fully natural green herbal medicine of principal agent, the first step prepares stem and leaf of Radix Astragali decocting liquid, first the parts by weight taking stem and leaf of Radix Astragali are 40, pulverize, then add 8 times amount water soaking 24 hours, extract 3 times, each 2 h, mixed extract, finally concentrates;
second step prepares Herba Taraxaci herb decocting liquid, and the parts by weight first taking Herba Taraxaci herb are 10, pulverize, and then adds 5 times amount water soaking 24 hours, and extract 3 times, each 2 h, mixed extract, finally concentrates;
3rd step prepares bracteal leaf of corn decocting liquid, and the parts by weight first taking bracteal leaf of corn are 10, pulverize, and then adds 10 times amount water soaking 24 hours, and extract 3 times, each 2 h, mixed extract, finally concentrates;
4th step prepares Caulis Momordicae Charantiae decocting liquid, and the parts by weight first taking Caulis Momordicae Charantiae are 10, pulverize, and then adds 8 times amount water soaking 24 hours, and extract 3 times, each 2 h, mixed extract, finally concentrates;
5th step is according to stem and leaf of Radix Astragali decocting liquid: Herba Taraxaci herb decocting liquid: bracteal leaf of corn decocting liquid: the ratio of Caulis Momordicae Charantiae decocting liquid is the ratio mixing of 6:2:1:1, and carrying out centrifugal treating, to obtain supernatant be stem and leaf of Radix Astragali compound recipe decocting liquid.
embodiment 14:
stem and leaf of Radix Astragali is a manufacture method for the fully natural green herbal medicine of principal agent, and the first step prepares stem and leaf of Radix Astragali decocting liquid, and the parts by weight first taking stem and leaf of Radix Astragali are 50, pulverize, then add 8 times amount water soaking 22 hours, extract 3 times, each 2 h, mixed extract, finally concentrates;
second step prepares Herba Taraxaci herb decocting liquid, and the parts by weight first taking Herba Taraxaci herb are 15, pulverize, and then adds 5 times amount water soaking 22 hours, and extract 3 times, each 2 h, mixed extract, finally concentrates;
3rd step prepares bracteal leaf of corn decocting liquid, and the parts by weight first taking bracteal leaf of corn are 15, pulverize, and then adds 10 times amount water soaking 22 hours, and extract 3 times, each 2 h, mixed extract, finally concentrates;
4th step prepares Caulis Momordicae Charantiae decocting liquid, and the parts by weight first taking Caulis Momordicae Charantiae are 15, pulverize, and then adds 8 times amount water soaking 22 hours, and extract 3 times, each 2 h, mixed extract, finally concentrates;
5th step is according to stem and leaf of Radix Astragali decocting liquid: Herba Taraxaci herb decocting liquid: bracteal leaf of corn decocting liquid: the ratio of Caulis Momordicae Charantiae decocting liquid is the ratio mixing of 6:2:1:1, and carrying out centrifugal treating, to obtain supernatant be stem and leaf of Radix Astragali compound recipe decocting liquid.
although, above illustrate and detailed description of the invention with general, to invention has been detailed elaboration, on basis of the present invention, can some amendments be made to it or improve.Therefore, amendment made without departing from theon the basis of the spirit of the present invention adds improvement, all belongs to the scope of protection of present invention.
Claims (5)
1. a stem and leaf of Radix Astragali is the fully natural green herbal medicine of principal agent, its composition comprises: stem and leaf of Radix Astragali, Herba Taraxaci herb, bracteal leaf of corn, Caulis Momordicae Charantiae, it is characterized in that: the parts by weight of described stem and leaf of Radix Astragali are 70 ~ 40, the parts by weight of described Herba Taraxaci herb are 20 ~ 10, the parts by weight of described bracteal leaf of corn are 20 ~ 10, and the parts by weight of described Caulis Momordicae Charantiae are 20 ~ 10.
2. stem and leaf of Radix Astragali according to claim 1 is the fully natural green herbal medicine of principal agent, it is characterized in that: the parts by weight of described stem and leaf of Radix Astragali are 60, the parts by weight of described Herba Taraxaci herb are 15, the parts by weight of described bracteal leaf of corn are 15, and the parts by weight of described Caulis Momordicae Charantiae are 15.
3. a stem and leaf of Radix Astragali is the manufacture method of the fully natural green herbal medicine of principal agent, it is characterized in that: the first step prepares stem and leaf of Radix Astragali decocting liquid, first the parts by weight taking stem and leaf of Radix Astragali are 70 ~ 40, pulverize, then add 8 times amount water soaking 20-24 hour, extract 3 times, each 2 h, mixed extract, finally concentrates;
Second step prepares Herba Taraxaci herb decocting liquid, and the parts by weight first taking Herba Taraxaci herb are 20 ~ 10, pulverize, and then adds 5 times amount water soaking 20-24 hour, and extract 3 times, each 2 h, mixed extract, finally concentrates;
3rd step prepares bracteal leaf of corn decocting liquid, and the parts by weight first taking bracteal leaf of corn are 20 ~ 10, pulverize, and then adds 10 times amount water soaking 20-24 hour, and extract 3 times, each 2 h, mixed extract, finally concentrates;
4th step prepares Caulis Momordicae Charantiae decocting liquid, and the parts by weight first taking Caulis Momordicae Charantiae are 20 ~ 10, pulverize, and then adds 8 times amount water soaking 20-24 hour, and extract 3 times, each 2 h, mixed extract, finally concentrates;
5th step is according to stem and leaf of Radix Astragali decocting liquid: Herba Taraxaci herb decocting liquid: bracteal leaf of corn decocting liquid: the ratio of Caulis Momordicae Charantiae decocting liquid is the ratio mixing of 6:2:1:1, and carrying out centrifugal treating, to obtain supernatant be stem and leaf of Radix Astragali compound recipe decocting liquid.
4. stem and leaf of Radix Astragali according to claim 3 is the manufacture method of the fully natural green herbal medicine of principal agent, it is characterized in that: the first step prepares stem and leaf of Radix Astragali decocting liquid, first the weight taking stem and leaf of Radix Astragali is 50g, pulverize, then add 8 times amount water soaking 20-24 hour, extract 3 times, each 2 h, mixed extract, finally concentrates;
Second step prepares Herba Taraxaci herb decocting liquid, and the weight first taking Herba Taraxaci herb is 50g, pulverizes, and then adds 5 times amount water soaking 20-24 hour, and extract 3 times, each 2 h, mixed extract, finally concentrates;
3rd step prepares bracteal leaf of corn decocting liquid, and the weight first taking bracteal leaf of corn is 50g, pulverizes, and then adds 10 times amount water soaking 20-24 hour, and extract 3 times, each 2 h, mixed extract, finally concentrates;
4th step prepares Caulis Momordicae Charantiae decocting liquid, and first taking Caulis Momordicae Charantiae weight is 50g, pulverizes, and then adds 8 times amount water soaking 20-24 hour, and extract 3 times, each 2 h, mixed extract, finally concentrates;
5th step is according to stem and leaf of Radix Astragali decocting liquid: Herba Taraxaci herb decocting liquid: bracteal leaf of corn decocting liquid: the ratio of Caulis Momordicae Charantiae decocting liquid is the ratio mixing of 6:2:1:1, and carrying out centrifugal treating, to obtain supernatant be stem and leaf of Radix Astragali compound recipe decocting liquid.
5. the stem and leaf of Radix Astragali according to claim 3 or 4 is the manufacture method of the fully natural green herbal medicine of principal agent, it is characterized in that: the described decocting cooking method that is extracted as extracts decocting liquid; Described simmer down to is concentrated into 2/3 of the decocting liquid weight of one procedure.
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| CN114431470A (en) * | 2022-01-14 | 2022-05-06 | 黑龙江维度壹健康管理股份有限公司 | Astragalus stem and leaf decoction and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114431470A (en) * | 2022-01-14 | 2022-05-06 | 黑龙江维度壹健康管理股份有限公司 | Astragalus stem and leaf decoction and preparation method thereof |
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