Summary of the invention
The purpose of this invention is to provide a kind of composite vegetables extractive and preparation method that can improve live pig immunity of organisms and premunition promotion growth.
Further aim of the present invention provides a kind of with the application process of described composite vegetables extractive as feed addictive.
The objective of the invention is to be achieved through the following technical solutions:
The present invention can improve the composite vegetables extractive that animal body immunity and premunition promote growth, and being mixed by the plant extracts of following weight proportion forms: camellia class plant accessory substance extract 5-8 part, membranous milkvetch stem and leaf extract 2-5 part, Herba Lophatheri extract 2-5 part, honeysuckle branches and leaves extract 1-3 part, Herba polygoni hydropiperis extract 1-3 part.
Described camellia class plant accessory substance is to be selected from least a in oil tea leaf, tea seed shell, the tea seed cake.
The present invention's composite vegetables extractive preparation method may further comprise the steps:
(1) camellia class plant accessory substance extract preparation: the camellia class plant accessory substance of drying is crushed to 2-5mm, add the water that the cellulase that is equivalent to camellia class plant accessory substance weight 0.8-1.2% and 9-11 doubly measure, under 65-70 ℃, enzymolysis 15-22h, filter, after filtrate decompression concentrated, spray-drying obtained camellia class plant accessory substance extract;
(2) membranous milkvetch stem and leaf extract preparation: the membranous milkvetch stem and leaf chopping with drying, no longer than 5cm, after the quick-fried processing of vapour, add flooding, extraction time 14-20h, temperature 92-98 ℃, to filter, after filtrate decompression concentrated, spray-drying obtained the membranous milkvetch stem and leaf extract;
(3) Herba Lophatheri extract preparation: the lophatherum gracile chopping with drying, no longer than 5cm, after the quick-fried processing of vapour, add flooding, extraction time 14-20h, temperature 92-98 ℃, to filter, filtrate decompression concentrates the back spray-drying and obtains Herba Lophatheri extract;
(4) honeysuckle branches and leaves extract preparation: the honeysuckle branches and leaves chopping with drying no longer than 5cm, adds flooding after the quick-fried processing of vapour, extraction time 15-20h, filters by temperature 92-98 ℃, after filtrate decompression concentrated, spray-drying obtained honeysuckle branches and leaves extract;
(5) Herba polygoni hydropiperis extract preparation: with the polygonum flaccidum chopping,, after the quick-fried processing of vapour, add flooding no longer than 5cm, extraction time 15-22h, temperature 92-98 ℃, to filter, after filtrate decompression concentrated, spray-drying obtained Herba polygoni hydropiperis extract;
(6) camellia class plant accessory substance extract 5-8 part, membranous milkvetch stem and leaf extract 2-5 part, Herba Lophatheri extract 2-5 part, honeysuckle branches and leaves extract 1-3 part, Herba polygoni hydropiperis extract 1-3 part are mixed.
Described camellia class plant accessory substance, its main active ingredient is Tea Polyphenols, tea polysaccharide, tea saponin, theophylline etc., have anti-oxidant, antibiotic, antiviral, strengthen effect such as immunity of organisms.
Described membranous milkvetch stem and leaf, its main active ingredient is polysaccharide, saponin(e etc., has to improve immunity of organisms, antibiotic, antiviral, antitumor, antifatigue, protect effect such as liver.
Described lophatherum gracile, its main active ingredient is flavonoids, saponin(e, polysaccharide etc., has effects such as heat-clearing, diuresis, sterilization.
Described honeysuckle branches and leaves, its main active ingredient has flavonoids, chlorogenic acid etc., has anti-inflammation, suppresses effects such as drug-fast bacteria protein is synthetic, enhancing immunologic function.
Described polygonum flaccidum, its main active ingredient are flavonoids, volatilization wet goods, have effects such as antibiotic, antiviral, anti-inflammatory, anti-oxidant, hemostasis, antitumor, analgesia.
Described composite vegetables extractive is added in the feed as feed addictive, and addition is that feed per ton adds 500-800g composite vegetables extractive powder.Concentrate feed, premix are pressed corresponding proportion and are added.Also can add 150-200g composite vegetables extractive powder, be mixed with the animal drinking water that contains composite vegetables extractive of the present invention, drink for animal by every 1000L water.
Test shows, in the pig child care stage, the present invention's composite vegetables extractive and a kind of antibiotics feed addictive are used, the result of use that multiple antibiotics feed addictive cooperates the high zinc feed additive of high-copper can be reached, also the result of use that the astragalus polyose feed addictive is used a kind of antibiotics feed addictive can be reached.Add product of the present invention in the fattening feed more than 35kg separately, effect is significantly better than the result of use of antibiotic combination and astragalus polyose.
Composite vegetables extractive of the present invention can improve swinery immunity, improves swinery health status, promotes the swinery growth, has the effect that improves meat flavor simultaneously.
Product of the present invention mainly extracts biological effective components from discarded or cheap agricultural byproducts, the adverse effect of having avoided striving medicine with the people, having cost an arm and a leg and easily fluctuate; Adopt aqueous enzymatic extraction, spray-drying, the multicomponent scientific matching, it is big to have solved the Chinese herbal medicine addition, uses problems such as inconvenience.
The specific embodiment
The invention will be further described below in conjunction with embodiment.
Embodiment 1
Each composition weight proportioning of present embodiment composite vegetables extractive: 6 parts of oil tea leaf extracts, 3 parts of membranous milkvetch stem and leaf extracts, 3 parts of Herba Lophatheri extracts, 2 parts of honeysuckle branches and leaves extracts, 2 parts of Herba polygoni hydropiperis extracts.
The preparation method is as follows:
(1) oil tea leaf extract preparation: the oil tea leaf of drying is crushed to 2-5mm, adds the cellulase that is equivalent to oil tea leaf weight 1% and the water of 10 times of amounts, 68 ℃ of enzymolysis 20h, then slag liquid is separated, after extract decompression concentrated, spray-drying, oil tea leaf extract powder;
(2) membranous milkvetch stem and leaf extract preparation: the membranous milkvetch stem and leaf chopping with drying no longer than 5cm, adds the water of 10 times of amounts after the quick-fried processing of vapour, at 95 ± 0.5 ℃ of lixiviate 16.5h, then slag liquid is separated, after solution decompression concentrates, spray-drying gets membranous milkvetch stem and leaf extract powder;
(3) Herba Lophatheri extract preparation: the lophatherum gracile chopping with drying no longer than 5cm, adds the water of 10 times of amounts after the quick-fried processing of vapour, at 95 ± 0.5 ℃ of lixiviate 18h, then slag liquid is separated, after solution decompression concentrates, spray-drying gets the Herba Lophatheri extract powder;
(4) honeysuckle branches and leaves extract preparation: the honeysuckle branches and leaves chopping with drying no longer than 5cm, adds the water of 10 times of amounts after the quick-fried processing of vapour, at 95 ± 0.5 ℃ of lixiviate 17h, then slag liquid is separated, after solution decompression concentrates, spray-drying,, get honeysuckle branches and leaves extract powder;
(5) Herba polygoni hydropiperis extract preparation: the polygonum flaccidum chopping with drying, no longer than 5cm, add the water of 10 times of amounts after the quick-fried processing of vapour, at 95 ± 0.5 ℃ of lixiviate 20h, then slag liquid is separated, after solution decompression concentrated, spray-drying got the Herba polygoni hydropiperis extract powder;
(6) with described camellia class plant extracts powder 6kg, membranous milkvetch stem and leaf extract powder 3kg, Herba Lophatheri extract powder 3kg, honeysuckle branches and leaves extract powder 2kg, Herba polygoni hydropiperis extract powder 2kg mixes, and obtains composite vegetables extractive powder 16kg.
Embodiment 2
Each composition weight proportioning of present embodiment composite vegetables extractive: camellia class plant accessory substance tea seed shell extract 5kg, membranous milkvetch stem and leaf extract 2kg, Herba Lophatheri extract 2kg, honeysuckle branches and leaves extract 1kg, Herba polygoni hydropiperis extract 1kg.
The preparation method is with embodiment 1.
Embodiment 3
Each composition weight proportioning of present embodiment composite vegetables extractive: camellia class plant accessory substance tea seed cake extract 8kg, membranous milkvetch stem and leaf extract 5kg, Herba Lophatheri extract 5kg, honeysuckle branches and leaves extract 3kg, Herba polygoni hydropiperis extract 3kg.
The preparation method is with embodiment 1.
Embodiment 4: the growth promotion application test
Experimental animal is the DLY tri-crossbreeding, each 4 hurdles of group house, and 4 pigs of each hurdle house, pilot time slot is divided into child care early stage, child care later stage, growth phase and fattening stage.
Test feed: test is divided into child care early stage, child care later stage, growth phase, fattening stage, it is identical that per stage is organized basal feed, add astragalus polyose, embodiment 1 product, antibiotic, zinc oxide in basal feed respectively, the interpolation situation sees Table 1 respectively, table 2, table 3, table 4.
Table 1 child care pre-stage test daily ration grouping unit: g/t
Project |
Control group |
Test I group |
Test II group |
Test III group |
Antibiotic I group |
Antibiotic II group |
1% that western peptide |
300 |
300 |
300 |
300 |
500 |
500 |
Astragalus polyose |
200 |
- |
- |
- |
- |
- |
Embodiment 1 product |
- |
400 |
600 |
800 |
- |
- |
10% colistin |
- |
- |
- |
- |
100 |
100 |
Zinc oxide |
- |
- |
- |
- |
- |
2000 |
Annotate: child care is meant that in earlier stage the test pig body weight stage is 10-20kg.
Table 2 child care later stage test daily ration grouping unit: g/t
Project |
Control group |
Test I group |
Test II group |
Test III group |
Antibiotic I group |
Antibiotic II group |
1% that western peptide |
300 |
300 |
300 |
300 |
- |
300 |
Astragalus polyose |
200 |
- |
- |
- |
- |
- |
Embodiment 1 product |
- |
400 |
600 |
800 |
- |
- |
10% colistin |
- |
- |
- |
- |
- |
300 |
50% kitasamycin |
- |
- |
- |
- |
120 |
- |
50% olaquindox |
- |
- |
- |
- |
120 |
- |
Annotate: the child care later stage is meant that the test pig body weight stage is 20-35kg.
Table 3 growth phase test daily ration grouping unit: g/t
Project |
Control group |
Test I group |
Test II group |
Test III group |
The antibiotic group |
Astragalus polyose |
200 |
- |
- |
- |
- |
Embodiment 1 product |
- |
400 |
600 |
800 |
- |
4% flavomycoin |
- |
- |
- |
- |
60 |
50% olaquindox |
- |
- |
- |
- |
80 |
Annotate: growth phase is meant that the test pig body weight stage is 35-55kg.
Table 4 fattening stage test daily ration grouping unit: g/t
Project |
Control group |
Test I group |
Test II group |
Test III group |
The antibiotic group |
Astragalus polyose |
200 |
- |
- |
- |
- |
Embodiment 1 product |
- |
400 |
600 |
800 |
- |
4% flavomycoin |
- |
- |
- |
- |
90 |
Annotate: fattening stage is meant that the test pig body weight stage is 55-90kg.
Result of the test:
Table 5 pair pig child care early growth Effect on Performance
Project |
Starting weight (kg) |
End heavy (kg) |
Daily gain (g) |
Daily ingestion amount (g) |
Feedstuff-meat ratio |
Diarrhea rate (%) |
Control group |
10.65±0.15 |
17.98±0.53 |
407±24
c |
733±24
b |
1.80±0.12 |
5.32
b |
Test I group |
10.68±0.27 |
18.37±1.12 |
427±68
b |
756±62
a |
1.77±0.19 |
8.33
a |
Test II group |
10.66±0.23 |
18.49±0.39 |
435±26
b |
753±42
a |
1.73±0.04 |
6.97
b |
Test III group |
10.66±0.18 |
18.72±0.55 |
448±24
a |
760±49
a |
1.70±0.04 |
6.48
b |
Antibiotic I group |
10.68±0.22 |
18.72±0.30 |
447±24
a |
765±31
a |
1.71±0.05 |
6.94
b |
Antibiotic II group |
10.55±0.48 |
18.78±0.27 |
457±15
a |
772±65
a |
1.69±0.13 |
1.85
c |
Annotate: the female different table differential of same column shoulder marking-up different significantly (
P<0.05), mark is not represented no significant difference; Down together.
As can be seen from Table 5, child care independent interpolation in early stage product of the present invention all is better than control group and promptly adds the astragalus polyose group, and test I group, test II group, test III group daily gain have improved 20g, 28g and 41g respectively, and daily ingestion amount and feedstuff-meat ratio be obviously improvement also; When this stage product addition of the present invention reaches 800g/t and cooperates a kind of antibiotic to use, growth performance is suitable with antibiotic I group, but three test group growth performances are all not as good as antibiotic II group, diarrhoea mainly occurred in one week of wean, the diarrhoea situation all was clearly better after each organized a week, and omnidistance diarrhea rate is to have added antibiotic II group the best of zinc oxide.
Table 6 pair pig child care late growing stage Effect on Performance
Project |
Starting weight (kg) |
End heavy (kg) |
Daily gain (g) |
Daily ingestion amount (g) |
Feedstuff-meat ratio |
Control group |
17.98±0.53 |
32.59±1.56 |
635±25 |
1190±24 |
1.87±0.12 |
Test I group |
18.37±1.12 |
32.98±2.57 |
635±52 |
1198±62 |
1.89±0.19 |
Test II group |
18.49±0.39 |
33.33±2.36 |
645±26 |
1202±42 |
1.86±0.04 |
Test III group |
18.72±0.55 |
33.62±0.82 |
648±23 |
1206±49 |
1.86±0.04 |
Antibiotic I group |
18.72±0.30 |
33.35±1.09 |
636±73 |
1193±31 |
1.88±0.05 |
Antibiotic II group |
18.78±0.27 |
33.52±0.61 |
641±15 |
1222±65 |
1.91±0.13 |
The result of the test of table 6 shows that at the child care later stage, it is suitable substantially that growth performance is respectively organized in test.
Table 7 pair growth INFLUENCE OF YUCCA EXTRACT
Project |
Starting weight (kg) |
End heavy (kg) |
Daily gain (g) |
Daily ingestion amount (kg) |
Feedstuff-meat ratio |
Control group |
31.93±0.45 |
53.55±1.47
b |
801±63
cd |
1.84±0.07 |
2.31±0.11 |
Test I group |
31.23±0.63 |
53.25±2.74
b |
816±103
c |
1.78±0.08 |
2.20±0.28 |
Test II group |
31.04±0.46 |
53.84±2.17
b |
844±96
b |
1.92±0.13 |
2.28±0.15 |
Test III group |
31.20±0.59 |
55.26±2.45
a |
891±88
a |
2.00±0.21 |
2.25±0.10 |
The antibiotic group |
30.98±0.33 |
51.68±1.71
c |
767±58
d |
1.85±0.09 |
2.42±0.12 |
Show by table 7 growth phase result of the test, add astragalus polyose, the growth performance of respectively organizing of product of the present invention all is better than the antibiotic group, add 600, the product group of the present invention of 800g/t significantly is better than adding the antibiotic group of 60g/t4% flavomycoin+80g/t50% olaquindox, child care early stage and child care later stage, it is not obvious with respect to the advantage of antibiotic group that product of the present invention is respectively organized growth performance, growth phase, advantage is fairly obvious, particularly test III group daily gain and exceed 90g than control group, exceed 124g than antibiotic group, show that composite vegetables extractive of the present invention need reach long-time its effect of use competence exertion more than 2 months.
Table 8 pair fattening INFLUENCE OF YUCCA EXTRACT
Project |
Starting weight (kg) |
End heavy (kg) |
Daily gain (g) |
Daily ingestion amount (kg) |
Feedstuff-meat ratio |
Control group |
53.55±1.47
b |
91.85±5.05
b |
851±34
b |
2.57±0.12 |
3.02±0.32 |
Test I group |
53.25±2.74
b |
90.92±2.88
b |
837±65
b |
2.51±0.20 |
3.00±0.25 |
Test II group |
53.84±2.17
b |
91.91±5.23
b |
846±54
b |
2.50±0.10 |
2.95±0.29 |
Test III group |
55.26±2.45
a |
96.12±2.83
a |
908±79
a |
2.65±0.24 |
2.92±0.18 |
The antibiotic group |
51.68±1.71
c |
87.55±4.93
c |
797±78
c |
2.48±0.17 |
3.11±0.42 |
The result of the test of table 8 shows, test III group growth performance obviously is better than other each groups, daily gain has improved 57g, 71g, 62g and 111g respectively, show that the follow-up effect of product of the present invention brings into play, control group, test are respectively organized growth performance and all are better than the antibiotic group, show that the follow-up effect of long-term use plant extracts is better than using for a long time antibiotic, plant extracts long more result of use service time is obvious more.
Embodiment 5: improve immunity of organisms, resistance against diseases test
Experimental design, daily ration grouping and composition are seen embodiment 4.
Result of the test:
The influence of table 9 pair child care pig sero-immunity index
Project |
IgG(g/l) |
IgM(g/l) |
Proleulzin (pg/ml) |
Gamma interferon (pg/ml) |
Control group |
4.10±0.62
a |
0.60±0.19
bc |
45.91±1.22
a |
62.15±2.01
b |
Test I group |
3.97±0.37
b |
0.55±0.11
c |
30.12±0.98
c |
57.41±1.94
b |
Test II group |
4.08±0.19
a |
0.67±0.03
bc |
39.07±2.01
b |
60.17±2.05
b |
Test III group |
4.12±0.32
a |
0.75±0.14
a |
37.56±0.87
ab |
68.42±1.52
a |
Antibiotic I group |
3.91±0.53
b |
0.62±0.19
c |
31.57±1.51
c |
50.49±0.17
c |
Antibiotic II group |
4.08±0.42
a |
0.67±0.23
ab |
35.22±1.25
bc |
55.37±2.07
bc |
Table 9 result of the test shows, when adding product volume of the present invention in the daily ration and reaching 800g/t, to compare every immune indexes the most desirable with other groups.
Embodiment 2,3 products have the 1 product similar effects with embodiment.