A kind of breeding method of weak-gluten wheat
Technical field
The invention belongs to crop breeding method and technology field, relate to a kind of breeding method of good quality and high output weak-gluten wheat.
Background technology
Weak-gluten wheat refers to that seed matter is soft, protein content is low, gluten strength is weak, extensibility is better, the wheat of the food such as suitable for making biscuit, cake and southern soft steamed bun.By the impact of the particularity of its quality responses, geological location and ecological factor etc., the weak-gluten wheat of high-quality is generally more suitable for producing in Mid-low latitude area, is only applicable to plantation soft red winter or soft white winter wheat in national some areas such as the U.S. and Australia abroad.According to " Wheat in China Quality Regionalization scheme " and " Regional Distribution of Superior Agriculture Products's planning ", China only has the Yangtze-Huai Plain of the middle and lower reach of Yangtze River to be suitable for the scale plantation of high-quality weak-gluten wheat, and the gross area is not enough, and national wheat broadcasts 10% of area.This district's soil is based on sandy soil---and sandy loam, weather is moistening, and heat condition is good, annual rainfall 800-1400mm, and overcast and rainy on the high side between the pustulation period, and illumination is on the weak side, and wheat seed protein accumulation amount is low, is conducive to the formation of seed plane of weakness muscle.The riverine region in Jiangsu Province and coastal sandy soil area region are the nucleus that middle and lower reach of Yangtze River weak-gluten wheat Pear dominant producing zone is built.
During the nearly last ten years, scientific research breeding unit of Jiangsu Province achieves breakthrough in the seed selection of weak-gluten wheat kind, be successively bred as peaceful wheat No. 9, raised wheat No. 9, raised wheat 13, peaceful wheat 13, raise wheat 15, raise wheat 18, raw select No. 6, peaceful wheat 18, raise the weak-gluten wheat kinds such as wheat 20.The development applied as China's weak-gluten wheat production of these kinds serves positive impetus.But along with improving constantly of Wheat Production level, produce upper, comprehensive resistance higher in the urgent need to yield potentiality more by force, the better wheat breed of quality.Therefore, the primary breeding objective that the disease-resistant weak muscle wheat special new varieties of good quality and high output are middle and lower reach of Yangtze River Mai Qu is cultivated.
Summary of the invention
The object of the invention is to overcome the defect existed in prior art, a kind of breeding method utilizing intervarietal cross to improve weak-gluten wheat quality, output and resistance is provided.The new strain of wheat cultivated by this breeding method weak muscle stay in grade, comprehensive resistance is good, wide adaptability, yield potentiality are large.
In order to achieve the above object, the invention provides following technical scheme:
A breeding method for weak-gluten wheat, comprises the following steps:
1) be maternal with the peaceful wheat of wheat breed No. 9, with high yield extensively suitable, comprehensive disease resistance is strong, the anti-fall wheat breed of semi-dwarf mutant for male parent, preparing hybrid combines, and obtains hybridization F1;
2) F1 is carried out breeding and produce F2, F3 two generation, combined hybrid plantation is all pressed by the hybrid population in F2, F3 generation, to the observation qualification that F2 and F3 carries out Agronomic characteristic, disease resistance, lodging resistance for colony, time ripe, select excellent single fringe mixing threshing, results seed.Due to second, third material that F2, F3 are hybridization, lower from generation to generation, the genotype of various proterties is in heterozygous state mostly, therefore, in this step to select fringe to be mixed into basis in generation.F1 results seed be F2, F2 results seed be F3, F3 results seed be F4;
3) all press combined hybrid plantation to F4 hybrid population, then carry out the observation qualification of economical character, disease resistance and lodging resistance, carry out ear selection, point fringe threshing time ripe, soft, the full single fringe seed of indoor selection seed is as F5; When cultivation arrived F4 for time, the genotype of most proterties is tending towards isozygotying, so this step adopts single fringe threshing after selecting fringe, single fringe kind of middle choosing becomes head progeny row F5, and now F5 is tending towards isozygotying substantially.
4) F5 kind is become head progeny row, select semi-dwarf mutant, lodging resistance is strong, comprehensive disease resistance is strong, economical character is excellent head progeny row according to variable rate technology, after results, carry out quality trait detection, select the head progeny row material meeting the weak-gluten wheat index of quality;
5) by select head progeny row F5 kind to become fringe system F6, select according to variable rate technology the fringe system F6 that moderate, comprehensive resistance of ripe phase is comparatively strong, economical character is excellent;
6) each for F6 excellent fringe system is planted into identify garden F7, measure grain yield, identify disease-resistant, lodging resistance, quality trait is measured comprehensively, finally select the excellent strain that output is high, comprehensive resistance strong, quality meets national weak-gluten wheat standard.
Further, in step 2) in, when F2 and F3 carries out selecting fringe mixing threshing, the excellent single fringe of every families selecting 400.
Further, in step 3) in, when F4 carries out selecting fringe to divide fringe threshing, 400-500 excellent single fringe is selected in each combination.
Traditionally, generally " pedigree method " and " bulk method " two kinds of systems of selection are adopted in wheat conventional breeding, what weak-gluten wheat breeding method provided by the invention adopted is improved group selection method, combine the advantage of bulk method and mass method of breeding, by selecting Parents preparing hybrid, utilizing genetic recombination and additive effect, creating excess of export parent offspring, by many for M8003 line, cultivate multiple high-quality weak-gluten wheat new lines.The weak muscle index of quality of these new lines and peaceful wheat No. 9 are quite or better, yield potentiality is significantly more than peaceful wheat No. 9, particularly plant height than peaceful wheat No. 9 short 7-8cm, and stalk crop is good, lodging resistance is strong, has certain popularizing application prospect or the parent management as genetic improvement of wheat.
Accompanying drawing explanation
Fig. 1 is breeding method flow chart of the present invention.
Embodiment
Below in conjunction with accompanying drawing of the present invention, be clearly and completely described technical scheme of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
As shown in Figure 1, the breeding method of a kind of weak-gluten wheat provided by the invention, comprises the following steps:
1) select the peaceful wheat of wheat breed No. 9 of low albumen, low gluten content weak muscle stay in grade for maternal in the spring of First Year, this kind have spend more many, comprehensive disease resistance is better, but stem stalk is comparatively thin, and lodging resistance is more weak; Selection comprehensive agronomy proterties is better, plant is shorter, comparatively thick, that lodging resistance is strong peaceful 0078 (naming afterwards as the peaceful wheat 13) wheat breed of stem stalk is male parent, and preparing hybrid combines; Hybridization F0 is planted in autumn planting in the same year, and the Second Year summer harvest obtains hybridization F1.
2) in the second to the five year, F1 is planted F2, F3 and F4 generation combination respectively by production density, and each generation, each family planted 15 row, long 3.6 meters of row, line-spacing 0.25 meter, the strain of each family colony about 4500.F2 and F3 is carried out to the observation qualification of Agronomic characteristic, disease resistance, the proterties such as resistant to lodging for colony, time ripe, every family gathers in the crops 400 excellent single fringes, mixing threshing, and adopts near-infrared analyzer to measure grain protein content to gathered in the crops seed;
3) F4 colony is carried out to the observation qualification of economical character and the proterties such as disease resistance, lodging resistance again, carry out ear selection time ripe, results 400-500 excellent single fringe, adopts a point fringe threshing, indoor selection full seed, soft single fringe seed.
3) excellent single fringe kind of middle choosing is become head progeny row F5 by the 5th year autumn, long 1.6 meters of row, line-spacing 0.25 meter.Carry out stooling seedling stage, cold resistance is selected; 6th year spring recorded heading, flowering stage; Grouting parameter carries out comprehensive resistance, economical character and yield traits and selects; Measure grain protein content, grain hardness and precipitation number after results, select grain protein content and precipitation number low, meet the head progeny row of the weak-gluten wheat index of quality.
4) the head progeny row kind of middle choosing is become fringe system F6 by the 6th year autumn, 7 row are planted by each fringe system, long 1.6 meters of row, line-spacing 0.25 meter, 1 contrast kind is planted every 10 fringe systems, select according to variable rate technology the fringe system that the ripe phase is moderate, comprehensive resistance is comparatively strong, economical character is excellent, and press fringe system mixing harvest-seed extraction, mensuration grain yield and quality respectively.Quality determination mainly comprises grain protein content, precipitation number and Solvent retention capacity.Pick out the excellent fringe system that in F6, yielding ability is better, quality reaches weak-gluten wheat index.
5) excellent fringe system recommends to enter Yield test garden (F7) by the 7th year autumn, qualification every part, garden strain plants 5 row, long 5 meters of row, line-spacing 0.25 meter, 1 contrast kind is planted every 10 strains, field test is disease-resistant, lodging resistance and economical character, grain yield is measured after results, adopt farinograph, tensilometer and the instrument that froths to measure flower characters and Cookie quality, finishing screen selects that output is high, comprehensive resistance strong, that quality meets national weak-gluten wheat standard is peaceful 1053, peaceful 1054, peaceful 1,055 three excellent Sister Lines participate in multiple spot varietal yield tests.
In 2011-2012 year multiple spot varietal yield test, peaceful 1053, peaceful 1054 and peaceful 1055 average yield per mus be respectively 470.5 kilograms, 488.0 kilograms and 462.0 kilograms, raise wheat 11 than contrast and increase production 10.48%, 14.52% and 8.48% respectively.The plant height of 3 strains is than the short 10-13cm of contrast, and fertilizer resistance anti-lodging is strong, Disease identification result, middle anti gibberellic disease, banded sclerotial blight and yellow mosaic, middle sense powdery mildew.Attributional analysis result, grain protein content 11.45%, 12.12%, 12.23%; Wet gluten content 21.2%, 22.1%, 23.0%; Dough stability time 1.9 minutes, 2.1 minutes, 2.4 minutes.
The breeding method of weak-gluten wheat provided by the invention, its three kinds obtained are peaceful 1053, peaceful 1054, peaceful 1055 output are high, comprehensive resistance strong, quality meets national weak-gluten wheat standard, plant height is short, stalk crop is good, lodging resistance is strong, middle and lower reach of Yangtze River Mai Qu can be generalized to as the weak muscle wheat special new varieties that good quality and high output is disease-resistant to apply, also can be used as the parent management of genetic improvement of wheat simultaneously.
The above; be only the specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, is anyly familiar with those skilled in the art in the technical scope that the present invention discloses; change can be expected easily or replace, all should be encompassed within protection scope of the present invention.Therefore, protection scope of the present invention should described be as the criterion with the protection domain of claim.