CN101313663A - Breeding method for corn selfing line M54 - Google Patents
Breeding method for corn selfing line M54 Download PDFInfo
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- CN101313663A CN101313663A CNA2008100122179A CN200810012217A CN101313663A CN 101313663 A CN101313663 A CN 101313663A CN A2008100122179 A CNA2008100122179 A CN A2008100122179A CN 200810012217 A CN200810012217 A CN 200810012217A CN 101313663 A CN101313663 A CN 101313663A
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Abstract
The invention relates to a method and a process for breeding a corn inbred line M54. The method comprises the following steps that: fine individuals of American hybrids 116R, 1140D, 1132X and German hybrid KWCP56 are selected; 50g fine individual of each hybrid is taken and evenly stirred, and then is stored in a parchment paper bag so as to be taken to a farmland; fine individuals of well growing hybrids including 1160R, 1140D, 1132X and KW456 are selected to carry out artificial bagging hybridization, and 8 to 10 ears of each fine individual undergo the hybridization; when harvested in autumn, all cross-bred ears are harvested through an artificial bagging method; from the end of October to the first ten days of November in the same year, prepared seed is taken to Hainan Province to carry out artificial single-grain spot sowing, and the harvested seed is sown in ear row in the north in next spring according to a pedigree method; and florescence is controlled through artificial bagging. According to the method, an M54 fine corn variety, which meets the requirements of excellent comprehensive economical character, strong comprehensive adversity resistance, high ear productivity of individual plant, greater grain depth, fine cob and high seed-producing percentage stated by the breeding object of super corn, is screened out through testcross combining at a sixth generation.
Description
Technical field
The invention belongs to the technical field of corn inbred lines method.
Background technology
The Maize Production of China has begun to promote single cross hybrid since the seventies, universal, the promotion and application of single cross hybrid have promoted China's corn area and output to increase considerably and have improved, add up according to interrelated data, the corn area is by 2.4 hundred million mu of the seventies, increase to present 4.2 hundred million mu, whole nation corn unit yield increases to present 375 kilograms by 180 kilograms of the seventies, and the unit yield in high yield province and high yield plot has reached more than 600 kilograms.The leading factor that corn yield improves is because the popularity rate that single cross hybrid is promoted has reached 95.6%, and the reason that single cross hybrid can promote corn yield to improve is by the seed selection of high-combining ability, high-level resistance and good economical character inbred line and the raising of improvement and crossbreed planting density.
Since the seventies, the corn inbred lines of China is used two traditional ring choosing systems and is accounted for more than 85%, the choosing of reestablishing diplomatic relations is and recurrent selection only accounts for about 10%, and because the restriction of human, financial, and material resources power, be restricted in the application facet of implementing large group, high density, high light efficiency, it is very little to take effect, to further raising breeding level, promote the update of new varieties, the development that promotes Maize Production has a significant impact.
Summary of the invention
The objective of the invention is to resource updates approach by introducing, digest and assimilate, innovating again, adopt two traditional rings of improvement to select system, method, enforcement is the breeding objective of purpose with the character improvement, it is the method that is selected from of M54 from height that a kind of corn is provided, and reaches to cultivate high yield, stable yields, high-quality, the wide purpose of suitable good new varieties.
The technical scheme that adopts is:
Utilize and introduce external good resource, change traditional simple two rings and select system, method, enforcement is successfully bred the good inbred line of M54 with the merit polymerization selection of the definite improvement of objective trait purpose, combines good beautiful No. 88 crossbreed that meet super Standard for Maize.
The selection of merit polymerization described in above-mentioned selection specified operational procedure is:
One, at first collects the pollen of the external corn hybrid seed meet the objective trait of improve purpose, carry out equivalent and stir, fully be mixed, mixing, select its fine individual plant again, carry out artificial pollination and implement to hybridize.
Selected the comprehensive agronomy proterties good, the pollen of the U.S. crossbreed 1160R that horizontal resistance is strong.
Selected the pollen of the U.S. crossbreed 1140D that the ripe phase is moderate, the tassel branch is few (1-3 bar), grain quality is good, seed-producing rate and unit weight are higher.
Selected that grain is dark, axle is thin, internode long, dredge blade profile, can improve the pollen of the U.S. crossbreed 1132X that light efficiency utilizes.
Selected the ripe phase early moderate partially, the fine individual plant pollen of colory German crossbreed KW456.
Two, the pollen of four external corn hybrid seeds that the field is gathered is taken indoor balance equivalent and is taken by weighing 50g, be put into stir in the glassware after, put into the sulfuric acid paper bag and bring the field into, select the fine individual plant of the crossbreed of 1160R, 1140D, 1132X and KW456 robust growth to carry out artificial bagging hybridization, respectively make the 8-10 fringe.
When three, gather in the crops autumn, will all gather in the crops by the fruit ear of artificial bagging method hybridization according to the method for selecting fruit ear after the first roguing, numbering is taken back indoor, treats to carry out the threshing species test behind the natural air drying, selects or eliminates according to the breeding objective of design in advance.Each kind keeps 5 tassels, the threshing of the perpendicular row of each tassel, and 200 of every ear selection typical case seeds then with this 20 fringe * 200, are counted 4000 seeds and are placed on and put into same nylon net bag after manually stirring in the container.
Four, 10 the end of month then to early November, take 4000 seeds getting ready to Hainan and implement artificial simple grain bunch plating in 1 mu of ground, treat its female fringe pedestrian worker's bagging control foreign pollen that advances of reeling off raw silk from cocoons, when the tassel for the treatment of 80% plant is bloomed loose powder, select the tassel of plant strain growth stalwartness, the comprehensive agronomy proterties is good, plant kenel difference the is big about 40-50 strain of dissimilar plant to put in strict accordance with the objective trait of super Standard for Maize again with the sulfuric acid paper bag, seal with paper clip simultaneously, prevent to be blown away or foreign pollen seals in.Isolate after 24 hours, artificially collect pollen, after the tassel that manually entangled the day before yesterday is cut with scissors, take back the indoor pollen of shaking off, weigh in the balance and get, each takes by weighing 10g, after implementing manually to stir, returns the fine individual plant that this colony is awarded in the field again.
Five, results the time are followed and to be selected excellent principle in excellent, continuation is carried out strict orientation according to objective trait and is selected, select fruit ear after selecting plant earlier, carry out the field numbering, bring back and carry out indoor species test after indoor natural wind is done, the fruit ear that does not meet super corn prescribed shape is eliminated, the plant plant type of selecting and remain compactness, internode length, blade upper punch, horizontal resistance strong, high anti--in anti-multiple leaf disease, fruit ear evenly, do not have empty stalk, not bald point, seed is dark, axle is thin, high, the quality better of seed-producing rate, the excellent fringe of excellent strain that unit weight is high, ordering numbering successively, single fringe threshing, single bag of preservation.
Six, become head progeny row in the north by the pedigree method kind spring next year, implement the standard plantation of high density (every mu 5000 strain), purpose is selected in order to realize under the artificial pressure conditions resistance such as anti-close, drought-enduring, anti-lean property.60 square metres of sub-district areas, line-spacing 60cm, 10 meters row are long, 10 row districts, spacing in the rows 22cm, every row is broadcast 46 strains, and each strain is sowed more than 400 strains.Artificial controlled water, fertile, anti-lean, drought-enduring, the anti-close property of the attention selection of control are implemented in field management.
Seven, the florescence is carried out artificial bagging control, the selected fine individual plant that meets objective trait carries out selfing, follow during results and select the principle of selecting excellent fringe after the excellent strain by 10% fruit ear of selecting and remain earlier, by the comprehensive agronomy The Characters numbering that sorts successively, single fringe is packed into and is carried out species test after mesh bag is taken back room-dry.Indoor passing the examination should show around the desired anti-dense planting of super corn, not have empty stalk, no small ear, not bald point, even, the about consistent field record of fruit ear in conjunction with the plant field, catch the fruit ear line number moderate, spike length and fringe are slightly suitable, and grain is dark, axle is thin, seed-producing rate is high, best in quality, unit weight is high, the high feature of single fringe productivity is estimated or select and remain.The spike number of selecting and remain determines with follow-on capacitive, generally should select and remain basis about 40 fringes in the field on, by eliminating about 50% again after the indoor species test of fruit ear, the 30%-50% that selects and remain, promptly the 10-20 fringe is advisable.
Eight, the 10-20 that the previous generation an is selected and remain head progeny row, taking Hainan to adds for planting into head progeny row, each head progeny row is multiple as far as possible, but many at least 200 strains, planting density, selection and superseded method must strict tightly be operated by said procedure, reach 7-8 generation until selfing, each head progeny row shows neat and consistent in the field, during the realistic application requirements of purity till.
Nine, in the operation that each head progeny row screening from generation to generation or excellent strain, excellent ear selection are selected, should firmly hold directed the selection, be that plant selects the strict ripe phase of grasp of palpus moderate, plant type is beneficial to high light efficiency utilization, lodging resistance is strong, that horizontal disease resistance reaches is high anti--in anti-standard, otherwise eliminate without exception, in order to reach this purpose, must 2-3 for the time carry out strange land screening and identify, S0 generation is in Dandong, in S1 generation, is in Hainan, in S2 generation, is in the Xunxian, Henan, S3 generation is in Changchun, Jilin, and S4 generation is returned Dandong and surveys synchronously and joins, and in time eliminates the not high head progeny row of coordinate force.
Ten, promptly from generation to generation to join the comprehensive agronomy proterties that meets super maize breeding defined that filters out by survey the 6th as stated above good for the M54 inbred line, comprehensive strong stress resistance, fruit ear individual plant productivity height, grain is dark, axle is thin, seed-producing rate is high, produce checking through the production of hybrid seeds, per mu yield surpasses 500 kilograms, and the high yield plot can reach more than 600 kilograms.
The selection of a kind of corn inbred line M54 of the present invention and the difference of conventional inbred line breeding method and innovative point such as following table:
| The selection of M54 | The selection of conventional inbred line |
| Multiple merit polymerization selection (polyphyly mixes powder) | Second cycle line selection (double-line hybrid) |
| Implementing artificial pressure selects in conjunction with pedigree method | The tradition pedigree method is selected |
| Carry out the strange land and plant selection more than 3 | Only plantation is selected on one point |
| Carry out comprehensive selection with multiple resistance in conjunction with eurytopicity | With coordinate force is that main separation is selected |
| The pursuit population yield is selected | The pursuit single plant yield is selected |
Adopt the present invention can cultivate the superior corn new varieties of high yield, stable yields, high-quality, wide adaptability.
Description of drawings
Fig. 1 is that corn of the present invention is a M54 seed selection collection of illustrative plates from height.
Detailed description of main elements:
1 is 1160R, 1140D, 1132X, the mixed powder of KW456 equivalent;
2,3,4 and 5 be respectively 1160R, 1140D, 1132X, KW456 select fine individual plant to hybridize;
6 for selecting good single fringe mixing threshing monoseeding;
7 hybridize with fine individual plant for the fine individual plant pollen mixture of gathering again;
8 for pressing pedigree method plantation head progeny row;
9 is 8 generations of continuous selfing;
10 is M54.
Embodiment
Embodiment one:
2007 is that the selection of M54 implement smart amount monoseeding breeding M54 parental inbred line by the corn of having narrated from height in the area, Tieling, density is 5050 strain/mus, line-spacing 60cm, spacing in the rows 22cm, actual cut output reaches 610 kilograms, and average single fringe reaches 0.12 kilogram.Increase production more than 30% than conventional inbred line.
Embodiment two:
2007 is that the selection of M54 produce trial-production kind by the corn of having narrated from height in the area, Jiuquan, planting density is 6200 strain/mus, female parent 5000 strain/mus of keeping a full stand of seedings wherein, the actual cut per mu yield reaches 500 kilograms more than, is more than 32% than first beautiful 335 the 378 kilograms of output increasing degrees of kind of the same type;
Embodiment three:
2007 is that the selection test of M54 survey produce result by the corn of having narrated from height through zone, Liaoning Province, with M54 is good beautiful No. 88 that female parent is hybridized assembly, average yield per mu reaches 683.1 kilograms, and comparison is according to kind of 585.7 kilograms of volume increase 16.6% of red beautiful No. 39 per mu yields, and the output rank occupies second.
Claims (1)
1, the selection of a kind of corn inbred line M54 is characterized in that the described following seed selection process that comprises:
1) selects U.S. crossbreed 116R pollen, 1140D pollen, 1132X pollen and German crossbreed KWCP56 fine individual plant pollen for use, get 50g for every kind, be placed on stir in the glass container after, put into the sulfuric acid paper bag and bring the field into, select the fine individual plant of the crossbreed of 1160R, 1140D, 1132X and KW456 robust growth to carry out artificial bagging hybridization, respectively make the 8-10 fringe;
When 2) gather in the crops autumn, will all gather in the crops by the fruit ear of artificial bagging method hybridization according to the method for selecting fruit ear after the first roguing, numbering is taken back indoor, treats to carry out the threshing species test behind the natural air drying, selects or eliminates according to the breeding objective of design in advance; Each kind keeps 5 tassels, the threshing of the perpendicular row of each tassel, and 200 of every ear selection typical case seeds then with this 20 fringe * 200, are counted 4000 seeds and are placed on and put into same nylon net bag after manually stirring in the container;
3) 10 the end of month then to early November, take 4000 seeds getting ready to Hainan and implement artificial simple grain bunch plating in 1 mu of ground, treat its female fringe pedestrian worker's bagging control foreign pollen that advances of reeling off raw silk from cocoons, when the tassel for the treatment of 80% plant is bloomed loose powder, select the tassel of plant strain growth stalwartness, the comprehensive agronomy proterties is good, plant kenel difference the is big about 40-50 strain of dissimilar plant to put in strict accordance with the objective trait of super Standard for Maize again with the sulfuric acid paper bag, seal with paper clip simultaneously, prevent to be blown away or foreign pollen seals in; Isolate after 24 hours, artificially collect pollen, after the tassel that manually entangled the day before yesterday is cut with scissors, take back the indoor pollen of shaking off, weigh in the balance and get, each takes by weighing 10g, after implementing manually to stir, returns the fine individual plant that this colony is awarded in the field again;
4) results the time are followed and to be selected excellent principle in excellent, continuation is carried out strict orientation according to objective trait and is selected, select fruit ear after selecting plant earlier, carry out the field numbering, bring back and carry out indoor species test after indoor natural wind is done, the fruit ear that does not meet super corn prescribed shape is eliminated, the plant plant type of selecting and remain compactness, internode length, blade upper punch, horizontal resistance strong, high anti--in anti-multiple leaf disease, fruit ear evenly, do not have empty stalk, not bald point, seed is dark, axle is thin, high, the quality better of seed-producing rate, the excellent fringe of excellent strain that unit weight is high, ordering numbering successively, single fringe threshing, single bag of preservation;
5) become head progeny row in the north by the pedigree method kind spring next year, implement the standard plantation of high density (every mu 5000 strain), 60 square metres of sub-district areas, line-spacing 60cm, 10 meters row are long, 10 row districts, spacing in the rows 22cm, every row is broadcast 46 strains, and each strain is sowed more than 400 strains; Artificial controlled water, fertile, anti-lean, drought-enduring, the anti-close property of the attention selection of control are implemented in field management;
6) florescence is carried out artificial bagging control, the selected fine individual plant that meets objective trait carries out selfing, follow during results and select the principle of selecting excellent fringe after the excellent strain by 10% fruit ear of selecting and remain earlier, by the comprehensive agronomy The Characters numbering that sorts successively, single fringe is packed into and is carried out species test after mesh bag is taken back room-dry; Indoor passing the examination should show around the desired anti-dense planting of super corn, not have empty stalk, no small ear, not bald point, even, the about consistent field record of fruit ear in conjunction with the plant field, catch the fruit ear line number moderate, spike length and fringe are slightly suitable, and grain is dark, axle is thin, seed-producing rate is high, best in quality, unit weight is high, the high feature of single fringe productivity is estimated or select and remain; The spike number of selecting and remain determines with follow-on capacitive, generally should select and remain basis about 40 fringes in the field on, by eliminating about 50% again after the indoor species test of fruit ear, the 30%-50% that selects and remain, promptly the 10-20 fringe is advisable;
7) 10-20 that the previous generation an is selected and remain head progeny row, taking Hainan to adds for planting into head progeny row, each head progeny row is multiple as far as possible, but many at least 200 strains, planting density, selection and superseded method must strict tightly be operated by said procedure, reach 7-8 generation until selfing, each head progeny row shows neat and consistent in the field, during the realistic application requirements of purity till;
8) in the operation that each head progeny row screening from generation to generation or excellent strain, excellent ear selection are selected, should firmly hold directed the selection, be that plant selects the strict ripe phase of grasp of palpus moderate, plant type is beneficial to high light efficiency utilization, lodging resistance is strong, that horizontal disease resistance reaches is high anti--in anti-standard, otherwise eliminate without exception, in order to reach this purpose, must 2-3 for the time carry out strange land screening and identify, S0 generation is in Dandong, in S1 generation, is in Hainan, in S2 generation, is in the Xunxian, Henan, S3 generation is in Changchun, Jilin, and S4 generation is returned Dandong and surveys synchronously and joins, and in time eliminates the not high head progeny row of coordinate force;
It is good from generation to generation to join the comprehensive agronomy proterties that meets super maize breeding defined that filters out by survey the 6th as stated above, comprehensive strong stress resistance, fruit ear individual plant productivity height, the M54 fine corn seeds that grain is dark, axle is thin, seed-producing rate is high.
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| CNA2008100122179A CN101313663A (en) | 2008-07-07 | 2008-07-07 | Breeding method for corn selfing line M54 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102884977A (en) * | 2012-10-18 | 2013-01-23 | 山西屯玉种业科技股份有限公司 | Breeding method of maize inbred line with high resistance, stable yield and high combining ability |
| CN104782478A (en) * | 2015-03-25 | 2015-07-22 | 河南农业大学 | Method for quickly and efficiently identifying high and stable-yield eurytopic new corn variety |
| CN106973785A (en) * | 2017-04-06 | 2017-07-25 | 漯河市农业科学院 | A kind of corn inbred line breeding method |
| CN112514791A (en) * | 2020-12-18 | 2021-03-19 | 云南点谷农业科技有限公司 | Breeding method for breeding maize inbred line through high-medium-low altitude cyclic planting |
-
2008
- 2008-07-07 CN CNA2008100122179A patent/CN101313663A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102884977A (en) * | 2012-10-18 | 2013-01-23 | 山西屯玉种业科技股份有限公司 | Breeding method of maize inbred line with high resistance, stable yield and high combining ability |
| CN104782478A (en) * | 2015-03-25 | 2015-07-22 | 河南农业大学 | Method for quickly and efficiently identifying high and stable-yield eurytopic new corn variety |
| CN106973785A (en) * | 2017-04-06 | 2017-07-25 | 漯河市农业科学院 | A kind of corn inbred line breeding method |
| CN112514791A (en) * | 2020-12-18 | 2021-03-19 | 云南点谷农业科技有限公司 | Breeding method for breeding maize inbred line through high-medium-low altitude cyclic planting |
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Application publication date: 20081203 |