CN104351034A - Artificial culture method of halophila oralis seedlings for marine ecological restoration - Google Patents
Artificial culture method of halophila oralis seedlings for marine ecological restoration Download PDFInfo
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- CN104351034A CN104351034A CN201410650358.9A CN201410650358A CN104351034A CN 104351034 A CN104351034 A CN 104351034A CN 201410650358 A CN201410650358 A CN 201410650358A CN 104351034 A CN104351034 A CN 104351034A
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- stolon
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
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Abstract
The invention relates to an artificial culture method of halophila oralis seedlings for marine ecological restoration, and belongs to the technical field of artificial culture of marine plant seedlings. According to the method, halophila oralis is cultured indoors to quickly obtain high-quality seedlings according to the reproduction characteristics that stem nodes on creeping stems of the halophila oralis can meristem and bifurcate and the creeping stems can horizontally extend and grow; the method specifically comprises the step of placing pruned creeping stem sections in seawater in which nutrient solution and a plant growth promotion hormone are added to culture for 25-30 days until the creeping stems extend horizontally to newly increase 2-3 stem nodes. The method has the characteristics of simple required equipment, convenience in material source, low production cost, easiness in mastering of the technology, horizontal extension of the creeping stems and high growth speed of buds on the stem nodes, the defect that the growth and the reproduction of the halophila oralis are affected by natural factors can be overcome, a large number of halophila oralis seedlings can be quickly obtained, the growth speed is high after the seedlings are transplanted to a sea area, and the protection and restoration effects on the halophila oralis are obvious.
Description
Technical field
The present invention relates to a kind of fingerlings artificial cultural method, be specifically related to a kind of happiness salt grass fingerlings artificial cultural method repaired for marine ecology, the sea grass fingerlings artificial cultural method of a kind of growth at subtidal zone, particularly to the marine eco-environment, there is repair function and water quality had to the sea grass of catharsis---happiness salt grass stolon manually cultivates to obtain a method for seedling, belongs to sea-plant fingerlings artificial culture technique field.
Background technology
Sea grass is the unifacial leaf herbaceous plant that a class lives in the littoral shallow water in marine site, temperate zone, general growth is at coastal subtidal zone, vast sea grass bed is often formed in the torrid zone and tropical coastal areas, sea grass bed is the same with mangrove forest and coral reef, three large typical marine ecosystems, the sea grass bed be made up of sea grass is the ecosystem-type that productivity is the highest, has important Ecosystem Service and economic worth, and far above mangrove forest and coral reef.
Sea grass is the food source that marine animal depends on for existence, as cowfish, sea urchin, conch, green turtle etc., just directly subsist on herbs, other marine animal is then sustain life by eating these " food grass " animals, simultaneously also for juvenile prawn, postlarva provide hidden and good protection place, look for food place and habitat; Due to the sea grass well developed root system of sea grass bed, be conducive to the erosion of storm resisting to offshore substrate, to marine benthos, there is protective effect.In ocean, sea grass bed can absorb the carbonic acid gas in water, there is huge carbon reserve capabillity, compare the absorptive amount of the annual 30000 tons of carbonic acid gas of the every sq-km of land forest, every sq-km sea grass bed can absorb 8.3 ten thousand tons of carbonic acid gas every year, the particularly important is, and is different from forest and is fixed with the wooden state of biology by carbonic acid gas, sea grass is stored in soil by carbonic acid gas, Gu the carbon time was more than more than thousand; Meanwhile, sea grass is by photosynthesis, and releasing oxygen is dissolved in water body, plays supplementary function to dissolved oxygen, effectively improves fisheries environment; Sea grass can be filtered the various sedimentss from land, and coast area can also be protected to be subject to the erosion of flood and storm tide less, maintains marine biodiversity.Therefore, sea grass marine ecosystems self-purification capacity, reduce seawater Eutrophic Extent and improve in marine environmental protection biocoenosis etc. there is important function.
Scientist finds, sea grass is very easily subject to the interference of natural calamity and mankind's activity.Along with the economic development of sea fishery and coastal area, intensive aquaculture of aquatic animal, the stretch of coastal water eutrophication that overfishing, environmental pollution cause, the unordered exploitation of human economy is to the destruction of sea grass habitat, and the increasing of the extreme weather such as climatic variation, typhoon, whole world sea grass area declines fast, sea grass grassland is annual at least to disappear with the speed of 1.5%, over nearly 10 years, whole world sea grass area disappears 30,000 sq-kms, and the original sea grass bed of 29% becomes extinct due to water quality deterioration.Fortunately, scientist finds, if protected, the recovery capability of sea grass bed is exceedingly fast, and they can Fast-propagation, re-establishes lost carbon absorbing capacity and other ecology service abilities.
Happiness salt grass is the important member of sea grass, and be subordinate to Hydrocharctaceae Halophila, it is coastal that China is mainly distributed in Hainan, Guangdong, Guangxi, Fujian and Hong Kong, is perennial sea grass species.Its stem is crawled, without vertical root-like stock, and tight knot on stem, often raw 1 radicula of joint, blade subcircular, ellipse or obovate, to life, flower unisexuality, dioecism, fruit is subsphaeroidal, seed is most, be a kind of small-sized, grow sea grass species rapidly, can to grow under middle tide brings to the depth of water of 60m, in soft shale or coral reef, to there is very strong Clonal growing ability, when after the large-scale feeding activity of generation, happiness salt grass seeds group is recovered rapidly by the expansion of horizontal rhizome.Although happiness salt grass has very strong Clonal growing ability; but by the restriction of marine environment; add that sea grass growth is at subtidal zone; most of the time floods in the seawater; seed collection is very difficult; the protection & restoration of sea grass bed is greatly affected, and happiness salt grass stolon efficiently, is fast cultivated, and is one of effective way solved the problem.Patent about happiness salt grass fingerlings artificial culturing yet there are no report.
Summary of the invention
The object of the present invention is to provide a kind of to the marine eco-environment have repair function and to seawater have catharsis happiness salt grass its seedling artificial culture method, the method equipment needed thereby is simple, material source is convenient, technology is easy to grasp, happiness salt grass seeds seedling can be obtained fast, in a large number, its fast growth behind happiness salt grass sprigging sea area, effectively can repair the marine eco-environment.
For realizing the object of foregoing invention, the technical scheme that the present invention takes is as follows:
For the happiness salt grass fingerlings artificial cultural method that marine ecology is repaired, comprise the steps:
(1) like the source of salt grass seed and select: after the sea area ebb growing salt grass in the family way, comparatively thick longer, that leaf growth is luxuriant, stipes the grows bud point happiness salt of stolon is selected careless from the subtidal zone in sea area, with fresh seawater, attached superincumbent silt and foreign material are rinsed well after gathering, stolon is cut into some segments, in order to cultivating;
(2) condition of culture of salt grass seed is liked: select white, capacity is the storage tank of 145 ~ 165L, clean up, after sterilization, add filtered fresh seawater (proportion 1.018 ~ 1.022) 120L ~ 130L, each storage tank adds nutritive salt mixed liquor 1 ~ 1.2 mL/L seawater that can promote to like the growth of salt grass, the rotten leachate 0.5 ~ 0.8mL/L seawater of fish and Plant growth-promoting effect hormone, after oxygenation stirs, the potassium permanganate soaking disinfection of the above-mentioned happiness salt grass stolon 5ppm being cut into segment 8 ~ 15 minutes, clean with seawater flushing, then be evenly put in water, every barrel of supply volume is 0.5 ~ 0.8kg,
(3) daily management of salt grass seed is liked: the seawater of stolon cultivation natural waters, control temperature and intensity of illumination, each storage tank is put a gas head and is carried out oxygenation in 24 hours, tolerance is adjusted to micro-boiling-like, if find that stolon flocks together or surfaces, gently stolon is separated with glass bar, it is allowed to be evenly distributed in water, add every other day to execute later and throw nutritive salt mixed liquor, within the 10th day, add the rotten leachate of fish, continue to keep stolon, grow fast;
(4) cultivation liking salt grass seed is ripe: stolon is cultivated 25 ~ 30 days, can increase 2 ~ 3 stipes newly to stolon horizontal extension, and the young shoot on former stipes generates the branch stolon with 2 ~ 3 stipes very soon.
Length and the specification of described every a bit of stolon are as follows: every a bit of stolon has 2 ~ 3 stipes, and on stipes, length has blade, bud point, length 3 ~ 4cm.
Described can promote the nutritive salt mixed liquor liking the growth of salt grass, containing following component: add NaNO in every 1000mL seawater
360 ~ 80g, K
2hPO
43 ~ 8g, FeC
6h
5o
7(solution of mass concentration 1%) 0.1 ~ 0.3mL, NaHCO
3200 ~ 400g, Cobastab
1150 ~ 250 μ g, Cobastab
2150 ~ 250m μ g.
Described Plant growth-promoting effect hormone is that pole can be apt to, plant nutrient agent and oligosaccharide element, and addition is that 0.8 ~ 1.2mg/5L seawater, plant nutrient agent 0.8 ~ 1.2mL/10L seawater, oligosaccharide element 0.3 ~ 0.8mL/10L seawater can be apt in pole respectively.
The rotten leachate of described fish, its preparation method is that the small fish of 500 grams is removed head, scale and internal organ, cleans up, be cut into small pieces and be put in sky mineral water bottle, add 2L fresh seawater, sealing and fermenting removes impurity with 100 object filter screens in 20 days afterwards, and filtrate is for subsequent use through high-temperature sterilization storage.
The Suitable ranges that described stolon is cultivated is 28 ~ 30 DEG C, and intensity of illumination remains on 5800 ~ 6000Lux.
The described mixed liquor of nutritive salt every other day adds and executes throwing amount is 0.1 ~ 0.3mL/L seawater, and the rotten leachate addition of fish is 0.2 ~ 0.3mL/L seawater.
The invention has the beneficial effects as follows: happiness salt grass stolon artificial culture method of the present invention, the impact of Growth and reproduction by natural cause of happiness salt grass can be overcome, stolon carries out large-scale artificial culture in indoor and obtains seedling, the method equipment needed thereby is simple, material source is convenient, young tooth reproduction speed on stolon horizontal extension and stipes is rapid, production cost is low, technology is easy to grasp, can be quick, a large amount of acquisition happiness salt grass seeds seedling, its fast growth behind sprigging sea area, the natural resources of energy available protecting happiness salt grass, repair the marine eco-environment, society, ecological benefits are remarkable.
Embodiment
Be described in further details the present invention below by example, these examples are only used for the present invention is described, do not limit the scope of the invention;
Embodiment 1
For the happiness salt grass fingerlings artificial cultural method that marine ecology is repaired, comprise the steps:
(1) like the source of salt grass seed and select: after the sea area ebb growing salt grass in the family way, comparatively thick longer, that leaf growth is luxuriant, stipes the grows bud point happiness salt of stolon is selected careless from the subtidal zone in sea area, with fresh seawater, attached superincumbent silt and foreign material are rinsed well after gathering, stolon is cut into some segments, in order to cultivating, length and the specification of every a bit of stolon are as follows: every a bit of stolon has 2 ~ 3 stipes, and on stipes, length has blade, bud point, length 3cm;
(2) condition of culture of salt grass seed is liked: selection white, capacity are the storage tank of 145L, after cleaning up, sterilizing, add filtered fresh seawater 120L, each storage tank adds nutritive salt mixed liquor 1 mL/L seawater, the rotten leachate 0.5mL/L seawater of fish and the Plant growth-promoting effect hormone that can promote to like the growth of salt grass, after oxygenation stirs, the potassium permanganate soaking disinfection of the above-mentioned happiness salt grass stolon 5ppm being cut into segment 8 minutes, clean with seawater flushing, then be evenly put in water, every barrel of supply volume is 0.5kg;
(3) daily management of salt grass seed is liked: the seawater of stolon cultivation natural waters, control temperature 28 DEG C and intensity of illumination 5800Lux, each storage tank is put a gas head and is carried out oxygenation in 24 hours, tolerance is adjusted to micro-boiling-like, if find that stolon flocks together or surfaces, gently stolon is separated with glass bar, it is allowed to be evenly distributed in water, add every other day to execute later and throw nutritive salt mixed liquor 0.1mL/L seawater, within 10th day, add fish rotten leachate 0.2mL/L seawater, continue to keep stolon, grow fast;
(4) cultivation liking salt grass seed is ripe: stolon cultivates 25 days, can increase 2 ~ 3 stipes newly to stolon horizontal extension, and the young shoot on former stipes generates the branch stolon with 2 ~ 3 stipes very soon;
Described can promote the nutritive salt mixed liquor liking the growth of salt grass, containing following component: add NaNO in every 1000mL seawater
360g, K
2hPO
43g, FeC
6h
5o
7(solution of mass concentration 1%) 0.1mL, NaHCO
3200g, Cobastab
1150 μ g, Cobastab
2150m μ g;
Described Plant growth-promoting effect hormone is that pole can be apt to, plant nutrient agent and oligosaccharide element, and addition is that 0.8 ~ 1.2mg/5L seawater, plant nutrient agent 0.8 ~ 1.2mL/10L seawater, oligosaccharide element 0.3 ~ 0.8mL/10L seawater can be apt in pole respectively;
The rotten leachate of described fish, its preparation method is that the small fish of 500 grams is removed head, scale and internal organ, cleans up, be cut into small pieces and be put in sky mineral water bottle, add 2L fresh seawater, sealing and fermenting removes impurity with 100 object filter screens in 20 days afterwards, and filtrate is for subsequent use through high-temperature sterilization storage;
Embodiment 2
For the happiness salt grass fingerlings artificial cultural method that marine ecology is repaired, comprise the steps:
(1) like the source of salt grass seed and select: after the sea area ebb growing salt grass in the family way, comparatively thick longer, that leaf growth is luxuriant, stipes the grows bud point happiness salt of stolon is selected careless from the subtidal zone in sea area, with fresh seawater, attached superincumbent silt and foreign material are rinsed well after gathering, stolon is cut into some segments, in order to cultivating, length and the specification of every a bit of stolon are as follows: every a bit of stolon has 2 ~ 3 stipes, and on stipes, length has blade, bud point, length 4cm;
(2) condition of culture of salt grass seed is liked: selection white, capacity are the storage tank of 165L, after cleaning up, sterilizing, add filtered fresh seawater 130L, each storage tank adds nutritive salt mixed liquor 1.2 mL/L seawater, the rotten leachate 0.8mL/L seawater of fish and the Plant growth-promoting effect hormone that can promote to like the growth of salt grass, after oxygenation stirs, the potassium permanganate soaking disinfection of the above-mentioned happiness salt grass stolon 5ppm being cut into segment 15 minutes, clean with seawater flushing, then be evenly put in water, every barrel of supply volume is 0.8kg;
(3) daily management of salt grass seed is liked: the seawater of stolon cultivation natural waters, control temperature 30 DEG C and intensity of illumination 6000Lux, each storage tank is put a gas head and is carried out oxygenation in 24 hours, tolerance is adjusted to micro-boiling-like, if find that stolon flocks together or surfaces, gently stolon is separated with glass bar, it is allowed to be evenly distributed in water, add every other day to execute later and throw nutritive salt mixed liquor 0.3mL/L seawater, within 10th day, add fish rotten leachate 0.3mL/L seawater, continue to keep stolon, grow fast;
(4) cultivation liking salt grass seed is ripe: stolon cultivates 30 days, can increase 2 ~ 3 stipes newly to stolon horizontal extension, and the young shoot on former stipes generates the branch stolon with 2 ~ 3 stipes very soon;
Described can promote the nutritive salt mixed liquor liking the growth of salt grass, containing following component: add NaNO in every 1000mL seawater
380g, K
2hPO
48g, FeC
6h
5o
7(solution of mass concentration 1%) 0.3mL, NaHCO
3400g, Cobastab
1250 μ g, Cobastab
2250m μ g;
Described Plant growth-promoting effect hormone is that pole can be apt to, plant nutrient agent, oligosaccharide element, and addition is that 0.8 ~ 1.2mg/5L seawater, plant nutrient agent 0.8 ~ 1.2mL/10L seawater, oligosaccharide element 0.3 ~ 0.8mL/10L seawater can be apt in pole respectively.
The rotten leachate of described fish, its preparation method is that the small fish of 500 grams is removed head, scale and internal organ, cleans up, be cut into small pieces and be put in sky mineral water bottle, add 2L fresh seawater, sealing and fermenting removes impurity with 100 object filter screens in 20 days afterwards, and filtrate is for subsequent use through high-temperature sterilization storage;
Embodiment 3
For the happiness salt grass fingerlings artificial cultural method that marine ecology is repaired, comprise the steps:
(1) like the source of salt grass seed and select: after the sea area ebb growing salt grass in the family way, comparatively thick longer, that leaf growth is luxuriant, stipes the grows bud point happiness salt of stolon is selected careless from the subtidal zone in sea area, with fresh seawater, attached superincumbent silt and foreign material are rinsed well after gathering, stolon is cut into some segments, in order to cultivating, length and the specification of every a bit of stolon are as follows: every a bit of stolon has 2 ~ 3 stipes, and on stipes, length has blade, bud point, length 3cm;
(2) condition of culture of salt grass seed is liked: selection white, capacity are the storage tank of 155L, after cleaning up, sterilizing, add filtered fresh seawater 125L, each storage tank adds nutritive salt mixed liquor 1.1 mL/L seawater, the rotten leachate 0.6mL/L seawater of fish and the Plant growth-promoting effect hormone that can promote to like the growth of salt grass, after oxygenation stirs, the potassium permanganate soaking disinfection of the above-mentioned happiness salt grass stolon 5ppm being cut into segment 12 minutes, clean with seawater flushing, then be evenly put in water, every barrel of supply volume is 0.7kg;
(3) daily management of salt grass seed is liked: the seawater of stolon cultivation natural waters, control temperature 29 DEG C and intensity of illumination 5900Lux, each storage tank is put a gas head and is carried out oxygenation in 24 hours, tolerance is adjusted to micro-boiling-like, if find that stolon flocks together or surfaces, gently stolon is separated with glass bar, it is allowed to be evenly distributed in water, add every other day to execute later and throw nutritive salt mixed liquor 0.2mL/L seawater, within 10th day, add fish rotten leachate 0.2mL/L seawater, continue to keep stolon, grow fast;
(4) cultivation liking salt grass seed is ripe: stolon cultivates 28 days, can increase 2 ~ 3 stipes newly to stolon horizontal extension, and the young shoot on former stipes generates the branch stolon with 2 ~ 3 stipes very soon;
Described can promote the nutritive salt mixed liquor liking the growth of salt grass, containing following component: add NaNO in every 1000mL seawater
370g, K
2hPO
45g, FeC
6h
5o
7(solution of mass concentration 1%) 0.2mL, NaHCO
3300g, Cobastab
1200 μ g, Cobastab
2200m μ g;
Described Plant growth-promoting effect hormone is that pole can be apt to, plant nutrient agent, oligosaccharide element, and addition is that 0.8 ~ 1.2mg/5L seawater, plant nutrient agent 0.8 ~ 1.2mL/10L seawater, oligosaccharide element 0.3 ~ 0.8mL/10L seawater can be apt in pole respectively;
The rotten leachate of described fish, its preparation method is that the small fish of 500 grams is removed head, scale and internal organ, cleans up, be cut into small pieces and be put in sky mineral water bottle, add 2L fresh seawater, sealing and fermenting removes impurity with 100 object filter screens in 20 days afterwards, and filtrate is for subsequent use through high-temperature sterilization storage;
Embodiment 4
For the happiness salt grass fingerlings artificial cultural method that marine ecology is repaired, comprise the steps:
(1) like the source of salt grass seed and select: after the sea area ebb growing salt grass in the family way, comparatively thick longer, that leaf growth is luxuriant, stipes the grows bud point happiness salt of stolon is selected careless from the subtidal zone in sea area, with fresh seawater, attached superincumbent silt and foreign material are rinsed well after gathering, stolon is cut into some segments, in order to cultivating, length and the specification of every a bit of stolon are as follows: every a bit of stolon has 2 ~ 3 stipes, and on stipes, length has blade, bud point, length 3cm;
(2) condition of culture of salt grass seed is liked: selection white, capacity are the storage tank of 150L, after cleaning up, sterilizing, add filtered fresh seawater 122L, each storage tank adds nutritive salt mixed liquor 1 mL/L seawater, the rotten leachate 0.6mL/L seawater of fish and the Plant growth-promoting effect hormone that can promote to like the growth of salt grass, after oxygenation stirs, the potassium permanganate soaking disinfection of the above-mentioned happiness salt grass stolon 5ppm being cut into segment 10 minutes, clean with seawater flushing, then be evenly put in water, every barrel of supply volume is 0.6kg;
(3) daily management of salt grass seed is liked: the seawater of stolon cultivation natural waters, control temperature 28 DEG C and intensity of illumination 5800Lux, each storage tank is put a gas head and is carried out oxygenation in 24 hours, tolerance is adjusted to micro-boiling-like, if find that stolon flocks together or surfaces, gently stolon is separated with glass bar, it is allowed to be evenly distributed in water, add every other day to execute later and throw nutritive salt mixed liquor 0.1mL/L seawater, within 10th day, add fish rotten leachate 0.2mL/L seawater, continue to keep stolon, grow fast;
(4) cultivation liking salt grass seed is ripe: stolon cultivates 26 days, can increase 2 ~ 3 stipes newly to stolon horizontal extension, and the young shoot on former stipes generates the branch stolon with 2 ~ 3 stipes very soon;
Described can promote the nutritive salt mixed liquor liking the growth of salt grass, containing following component: add NaNO in every 1000mL seawater
365g, K
2hPO
44g, FeC
6h
5o
7(solution of mass concentration 1%) 0.1mL, NaHCO
3250g, Cobastab
1180 μ g, Cobastab
2180m μ g;
Described Plant growth-promoting effect hormone is that pole can be apt to, plant nutrient agent, oligosaccharide element, and addition is that 0.8 ~ 1.2mg/5L seawater, plant nutrient agent 0.8 ~ 1.2mL/10L seawater, oligosaccharide element 0.3 ~ 0.8mL/10L seawater can be apt in pole respectively;
The rotten leachate of described fish, its preparation method is that the small fish of 500 grams is removed head, scale and internal organ, cleans up, be cut into small pieces and be put in sky mineral water bottle, add 2L fresh seawater, sealing and fermenting removes impurity with 100 object filter screens in 20 days afterwards, and filtrate is for subsequent use through high-temperature sterilization storage.
Embodiment 5
For the happiness salt grass fingerlings artificial cultural method that marine ecology is repaired, comprise the steps:
(1) like the source of salt grass seed and select: after the sea area ebb growing salt grass in the family way, comparatively thick longer, that leaf growth is luxuriant, stipes the grows bud point happiness salt of stolon is selected careless from the subtidal zone in sea area, with fresh seawater, attached superincumbent silt and foreign material are rinsed well after gathering, stolon is cut into some segments, in order to cultivating, length and the specification of every a bit of stolon are as follows: every a bit of stolon has 2 ~ 3 stipes, and on stipes, length has blade, bud point, length 4cm;
(2) condition of culture of salt grass seed is liked: selection white, capacity are the storage tank of 160L, after cleaning up, sterilizing, add filtered fresh seawater 128L, each storage tank adds nutritive salt mixed liquor 1.2 mL/L seawater, the rotten leachate 0.7mL/L seawater of fish and the Plant growth-promoting effect hormone that can promote to like the growth of salt grass, after oxygenation stirs, the potassium permanganate soaking disinfection of the above-mentioned happiness salt grass stolon 5ppm being cut into segment 13 minutes, clean with seawater flushing, then be evenly put in water, every barrel of supply volume is 0.7kg;
(3) daily management of salt grass seed is liked: the seawater of stolon cultivation natural waters, control temperature 30 DEG C and intensity of illumination 6000Lux, each storage tank is put a gas head and is carried out oxygenation in 24 hours, tolerance is adjusted to micro-boiling-like, if find that stolon flocks together or surfaces, gently stolon is separated with glass bar, it is allowed to be evenly distributed in water, add every other day to execute later and throw nutritive salt mixed liquor 0.3mL/L seawater, within 10th day, add fish rotten leachate 0.3mL/L seawater, continue to keep stolon, grow fast;
(4) cultivation liking salt grass seed is ripe: stolon cultivates 29 days, can increase 2 ~ 3 stipes newly to stolon horizontal extension, and the young shoot on former stipes generates the branch stolon with 2 ~ 3 stipes very soon;
Described can promote the nutritive salt mixed liquor liking the growth of salt grass, containing following component: add NaNO in every 1000mL seawater
375g, K
2hPO
47g, FeC
6h
5o
7(solution of mass concentration 1%) 0.3mL, NaHCO
3350g, Cobastab
1220 μ g, Cobastab
2220m μ g;
Described Plant growth-promoting effect hormone is that pole can be apt to, plant nutrient agent, oligosaccharide element, and addition is that 0.8 ~ 1.2mg/5L seawater, plant nutrient agent 0.8 ~ 1.2mL/10L seawater, oligosaccharide element 0.3 ~ 0.8mL/10L seawater can be apt in pole respectively;
The rotten leachate of described fish, its preparation method is that the small fish of 500 grams is removed head, scale and internal organ, cleans up, be cut into small pieces and be put in sky mineral water bottle, add 2L fresh seawater, sealing and fermenting removes impurity with 100 object filter screens in 20 days afterwards, and filtrate is for subsequent use through high-temperature sterilization storage.
Claims (7)
1., for the happiness salt grass fingerlings artificial cultural method that marine ecology is repaired, it is characterized in that: comprise the steps:
(1) like the source of salt grass seed and select: after the sea area ebb growing salt grass in the family way, comparatively thick longer, that leaf growth is luxuriant, stipes the grows bud point happiness salt of stolon is selected careless from the subtidal zone in sea area, with fresh seawater, attached superincumbent silt and foreign material are rinsed well after gathering, stolon is cut into some segments, in order to cultivating;
(2) cultivation of salt grass seed is liked: select white, capacity is the storage tank of 145 ~ 165L, clean up, after sterilization, add filtered fresh seawater 120L ~ 130L, each storage tank adds nutritive salt mixed liquor 1 ~ 1.2 mL/L seawater that can promote to like the growth of salt grass, the rotten leachate 0.5 ~ 0.8mL/L seawater of fish and Plant growth-promoting effect hormone, after oxygenation stirs, the potassium permanganate soaking disinfection of the above-mentioned happiness salt grass stolon 5ppm being cut into segment 8 ~ 15 minutes, clean with seawater flushing, then be evenly put in water, every barrel of supply volume is 0.5 ~ 0.8kg,
(3) daily management of salt grass seed is liked: the seawater of stolon cultivation natural waters, control temperature and intensity of illumination, each storage tank is put a gas head and is carried out oxygenation in 24 hours, tolerance is adjusted to micro-boiling-like, and stolon flocks together or surfaces, and gently stolon is separated with glass bar, it is allowed to be evenly distributed in water, add every other day to execute later and throw nutritive salt mixed liquor, within the 10th day, add the rotten leachate of fish, continue to keep stolon, grow fast;
(4) cultivation liking salt grass seed is ripe: stolon is cultivated 25 ~ 30 days, can increase 2 ~ 3 stipes newly to stolon horizontal extension, and the young shoot on former stipes generates the branch stolon with 2 ~ 3 stipes very soon.
2. a kind of happiness salt grass fingerlings artificial cultural method repaired for marine ecology according to claim 1, it is characterized in that: the stolon described in step (1) is cut into some segments, length and the specification of every a bit of stolon are as follows: every a bit of stolon has 2 ~ 3 stipes, and length has blade, bud point on stipes, length 3 ~ 4cm.
3. a kind of happiness salt grass fingerlings artificial cultural method repaired for marine ecology according to claim 1, is characterized in that: can promote described in step (2) likes the nutritive salt mixed liquor of salt grass growth, containing following component: add NaNO in every 1000mL seawater
360 ~ 80g, K
2hPO
4the FeC of 3 ~ 8g, mass concentration 1%
6h
5o
70.1 ~ 0.3mL, NaHCO
3200 ~ 400g, Cobastab
1150 ~ 250 μ g, Cobastab
2150 ~ 250m μ g.
4. a kind of happiness salt grass fingerlings artificial cultural method repaired for marine ecology according to claim 1, it is characterized in that: the Plant growth-promoting effect hormone described in step (2) is that pole can be apt to, plant nutrient agent and oligosaccharide element, and its addition is that 0.8 ~ 1.2mg/5L seawater, plant nutrient agent 0.8 ~ 1.2mL/10L seawater, oligosaccharide element 0.3 ~ 0.8mL/10L seawater can be apt in pole respectively.
5. a kind of happiness salt grass fingerlings artificial cultural method repaired for marine ecology according to claim 1, it is characterized in that: the rotten leachate of the fish described in step (2), its preparation method is that the small fish of 500 grams is removed head, scale and internal organ, clean up, be cut into small pieces and be put in sky mineral water bottle, add 2L fresh seawater, sealing and fermenting removes impurity with 100 object filter screens in 20 days afterwards, and filtrate can obtain the rotten leachate of fish through high-temperature sterilization.
6. a kind of happiness salt grass fingerlings artificial cultural method repaired for marine ecology according to claim 1, it is characterized in that: the control temperature described in step (3) and intensity of illumination are: the Suitable ranges that stolon is cultivated is 28 ~ 30 DEG C, and intensity of illumination remains on 5800 ~ 6000Lux.
7. a kind of happiness salt grass fingerlings artificial cultural method repaired for marine ecology according to claim 1, it is characterized in that: adding every other day described in step (3) is executed and thrown nutritive salt mixed liquor and add that to execute throwing amount be 0.1 ~ 0.3mL/L seawater, and the rotten leachate addition of fish is 0.2 ~ 0.3mL/L seawater.
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