CN104288284A - Traditional Chinese medicine compound hydro-acupuncture preparation for preventing and curing fever and plague of livestock and poultry and preparation method thereof - Google Patents
Traditional Chinese medicine compound hydro-acupuncture preparation for preventing and curing fever and plague of livestock and poultry and preparation method thereof Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/35—Caprifoliaceae (Honeysuckle family)
- A61K36/355—Lonicera (honeysuckle)
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/19—Acanthaceae (Acanthus family)
- A61K36/195—Strobilanthes
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/288—Taraxacum (dandelion)
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
- A61K36/315—Isatis, e.g. Dyer's woad
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
- A61K36/704—Polygonum, e.g. knotweed
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/74—Rubiaceae (Madder family)
- A61K36/744—Gardenia
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/85—Verbenaceae (Verbena family)
- A61K36/855—Clerodendrum, e.g. glorybower
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- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
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- A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/70—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in livestock or poultry
Abstract
The invention relates to a traditional Chinese medicine compound hydro-acupuncture preparation for preventing and curing fever and plague of livestock and poultry. The traditional Chinese medicine compound hydro-acupuncture preparation for preventing and curing fever plague of livestock and poultry is prepared from the following drug substances in parts by weight: 15-20 parts of honeysuckle, 50-70 parts of dandelion, 20-30 parts of cape jasmine and 25-35 parts of folium isatidis. The traditional Chinese medicine compound preparation provided by the invention is nontoxic, harmless, and inexpensive in the price; the medication is safe and the using effect is remarkable.
Description
Technical field
The invention belongs to biological pharmacy technical field for animals, be specifically related to a kind ofly prevent and treat Chinese medicine compound hydro-acupuncture preparation of poultry distemper disease and preparation method thereof.
Background technology
Virus is all kinds of animal of invasion and attack extensively, no exception to economic animal, of common occurrence in poultry or livestock breeding.Pestilence virus and foot and mouth disease virus be wherein coverage very extensively, cause the two-strain that economic loss is comparatively serious.Some virus such as mice Avian pneumo-encephalitis virus, swine fever virus, rabbit hemorrhagic fever virus, psittacosis virus and rabies virus are also formidable.
The symptom of mice newcastle: sick mice is a cup too low, dyspnea, weak.It is serious that visceroatonia type shows as diarrhoea, do not eat slow-witted vertical, exhaustion; Can be there is the torsion of clonospasm, neck, opisthotonus and lower limb paralysis in resistance to mice excessively, some wing paralysis, mortality rate can reach more than 90%.Nervous system type shows as loss of appetite, cough, and gas is kicked, and head and neck stretches mouth breathing; Occur wryneck, turn-take, two wings and two lower limbs paralysis, walk unstable, young mouse death rate can reach more than 90%.
The symptom of mice Marek: the dyskinesia appears in sick mice, in side property or both sides property paralysis, namely in one foot stretch to front, another foot stretch rearward characteristic split position; The sick mice had also has two wings sagging, torticollis of bowing, the symptoms such as gait is overcautious.
Avian infectious encephalomyelitis: young mice is multiple, sick young slow-witted blunt, send out straight, be reluctant to walk about for two, squat sleeping; Clinically there is movement disorder, not exclusively benumb, two wing muscle paresis and incidence muscular tremor etc. are cardinal symptom.
The arthritic symptom of Murine Virus: sick mice develop is bad, walking lamely or foot multitudinous portion bending deformation, appear in tarsal joint obvious tumefaction; Sick mice happiness is sitting on joint, just beats when driveing; Suffering limb can not be upheld and bear a heavy burden, and serious suffering limb is outwards reversed, and lurchs.
The symptom of mice infectious bursal disease: sick mouse primary, from pecking perianal feather, is suffered from diarrhoea, draining sample loose stool; Trembling appears in some mice bodies, rocks on foot, instability of gait.Later stage, sick mouse diet went down, and two wings are sagging, and feather is inverse vertical matt; Some heads with hanging down, are closed one's eyes in lethargy shape.
Summary of the invention
For above-mentioned prior art Problems existing, the invention provides a kind of Chinese medicine compound hydro-acupuncture preparation preventing and treating poultry distemper disease, this Chinese medicine compound hydro-acupuncture preparation is nontoxic, harmless, cheap, and drug safety, result of use is remarkable.
In order to achieve the above object, the present invention adopts following technical scheme:
The invention provides a kind of Chinese medicine compound hydro-acupuncture preparation preventing and treating poultry distemper disease, be prepared from by the crude drug of following weight portion: Flos Lonicerae 15 ~ 20 parts, Herba Taraxaci 50 ~ 70 parts, Fructus Gardeniae 20 ~ 30 parts and Folium Isatidis 25 ~ 35 parts.
According to the Chinese medicine compound hydro-acupuncture preparation of above-mentioned control poultry distemper disease, be prepared from by the crude drug of following weight portion: Flos Lonicerae 17 ~ 19 parts, Herba Taraxaci 58 ~ 62 parts, Fructus Gardeniae 23 ~ 25 parts and Folium Isatidis 28.5 ~ 31.5 parts.
According to the Chinese medicine compound hydro-acupuncture preparation of above-mentioned control poultry distemper disease, be prepared from by the crude drug of following weight portion: Flos Lonicerae 18 parts, Herba Taraxaci 60 parts, Fructus Gardeniae 24 parts and Folium Isatidis 29 parts.
According to the Chinese medicine compound hydro-acupuncture preparation of above-mentioned control poultry distemper disease, described Chinese medicine compound hydro-acupuncture preparation is oral liquid formulation or injection dosage form.
According to the preparation method of the Chinese medicine compound hydro-acupuncture preparation of control poultry distemper disease described above, comprise the steps:
(1) pretreatment of crude drug: by the remove impurity respectively of Flos Lonicerae, Herba Taraxaci, Fructus Gardeniae and Folium Isatidis, dry, being ground into granularity is that 40 ~ 160 order fine powders are for subsequent use;
(2) take Flos Lonicerae, Herba Taraxaci, Fructus Gardeniae and Folium Isatidis according to above-mentioned formula, add flooding three times, the first time lixiviate water soaking 30 ~ 40min of 12 times of Flos Lonicerae weight, slow fire boiling 40 hours, filters, obtains the first filtrate; The water that first time filtration medicinal residues add 10 times of Flos Lonicerae weight carries out second time lixiviate, slow fire boiling 30 hours, filters, obtains the second filtrate; The water that second time filtration medicinal residues add 10 times of Flos Lonicerae weight again carries out third time lixiviate, slow fire boiling 30min, filters, obtains the 3rd filtrate, merge the first filtrate, the second filtrate and the 3rd filtrate;
(3) the filtrate centrifugal treating after step (2) being merged obtains supernatant, and supernatant is carried out microfiltration, ultrafiltration, and the filtrate after ultrafiltration is 1.2 ~ 1.36 through being evaporated to relative density;
(4) medicinal liquid adding distil water after step (3) concentrating under reduced pressure is settled to amount of preparation, fill, sterilizing get product.
Chinese medicine compound hydro-acupuncture preparation of the present invention, usage and dosage is as follows:
Oral liquid 1 ~ 2mL is watered 800g, injection pig, cattle, sheep 0.15ml/kg, chicken, duck 0.1ml/kg.
As seen from the above, Chinese medicine compound hydro-acupuncture preparation of the present invention is in the application of the sick function aspects of control poultry distemper; The application of the sick function aspects of the domestic animal distemper such as the poultry such as chicken, duck and pig, cattle, sheep can be prevented and treated.
Modern Chinese medicine pharmacological research:
Flos Lonicerae: property is sweet, cold, return lung, the heart, stomach warp.Function with cure mainly: heat-clearing and toxic substances removing, dispelling wind and heat pathogens.For carbuncle furuncle, sore throat, erysipelas, toxic-heat and blood stasis, anemopyretic cold, epidemic febrile disease is generated heat.
Herba Taraxaci: property is bitter, sweet, cold, returns liver, stomach warp.Function with cure mainly: heat-clearing and toxic substances removing, dispersing swelling and dissipating binds, inducing diuresis for treating stranguria syndrome.For furuncle swelling toxin, acute mastitis, scrofula, conjunctival congestion, pharyngalgia, lung abscess, acute appendicitis, jaundice due to damp-heat, the puckery pain of pyretic stranguria.
Fructus Gardeniae: property is bitter, cold, GUIXIN, lung, tri-jiao channel.Function with cure mainly: pathogenic fire purging relieving restlessness, clearing away heat-damp and promoting diuresis, removing pathogenic heat from blood and toxic substance from the body; Externally used detumescence pain relieving.Vexed for calentura, jaundice due to damp-heat, the puckery pain of stranguria, heat in blood tells nosebleed, conjunctival congestion and swelling pain, pathogenic fire,toxin and furuncles, external treatment bruise pain.
Folium Isatidis: property is bitter, cold, GUIXIN, stomach warp.Function with cure mainly: heat-clearing and toxic substances removing, blood cooling and ecchymoses removing.For the high heat of epidemic febrile disease, coma, sends out speckle dermexanthesis, mumps, sore throat, erysipelas, carbuncle.
Flos Lonicerae pharmacological action: 1, resisting pathogenic microbes effect: experiment in vitro shows, flower and rattan to various pathogens Flos Lonicerae as staphylococcus aureus, Hemolytic streptococcus, escherichia coli, dysentery bacterium, vibrio cholera, Bacillus typhi, Salmonella paratyphi etc. all have certain inhibitory action, also effective to streptococcus pneumoniae, meningococcus, bacillus pyocyaneus, tubercule bacillus.Water logging agent is stronger than decoct effect, and leaf decoct is stronger than colored decoct effect.If with Fructus Forsythiae share, antibacterial range also can be complementary; Share with penicillin, can strengthen the antibacterial action of penicillin to resistant Staphylococcus aureus, this may be, in anti-bacteria body, white matter synthesis has collaborative effect.2, antiinflammatory and refrigeration function: lumbar injection Flos Lonicerae extractive solution 0.25g/kg, can suppress Rat Carrageenan colloidality swelling of the feet.3, booster immunization function effect: Flos Lonicerae decoct is diluted to the concentration of 1:1280, still can promote leukocytic phagocytic function.Mouse peritoneal injection honeysuckle flower injection, also has the effect obviously promoting inflammatory cell phagocytic function.4, central excitation effect: after the kinds of experiments such as galvanic shock, rotating cage method proves oral chlorogenic acid, the animal central nervous systems such as rat, mice can be caused excited, and its action intensity is 1/6 of caffeine, and Papillary is without addition and potentiation.5, effect for reducing blood fat: rat oral gavage Flos Lonicerae 2.5g/kg can reduce enteral cholesterol absorption, reduces cholesterol level in blood plasma.Experiment in vitro also finds that Flos Lonicerae can combine with cholesterol, but SIMIAOYONGAN TANG (Flos Lonicerae, Radix Scrophulariae, Radix Angelicae Sinensis, Radix Glycyrrhizae) treats Experimental Atherosclerosis in Rabbit.Do not observe the effect of blood fat reducing and Cholesterol concentration in aortic tissue content.6, antiendotoxin: measure endotoxin content with limulus test, 300% honeysuckle flower injection dilutes with 1:2 ~ 1:64, no matter in vitro tests uses female tab method or test tube method, all obviously reduce the endotoxin content in test solution, wherein the dilution tube of 1:2 ~ 1:8 is the same with negative control pipe is in a liquid state, and positive control is gel.
The chemical composition Herba Taraxaci herb of Herba Taraxaci is containing taraxasterol, choline, inulin, pectin etc.; Containing taraxol, taraxerol, ψ-Portugal's Herba Taraxaci sterol, taraxasterol, β-Amyrin, stigmasterol, cupreol, choline, organic acid, fructose, sucrose, glucose, glycoside and resin, rubber etc. in root.Leaf is containing phylloxanthin, violaxanthin, phylloquinone, vitamin C and vitamin D; Containing arnica montana glycol, phylloxanthin and flavoxanthin in spending.Containing cupreol, 5z-bean steroid-7-alkene-3 β-ol, folic acid and vitamin C in pollen.Containing phylloquinone in green calyx.Containing cupreol and β-Amyrin in scape.The pharmacological action of Herba Taraxaci: (1) antibacterial action: this product injection in vitro has stronger bactericidal action to staphylococcus aureus resistance bacterial strain, Hemolytic streptococcus.Also certain bactericidal action is had to Diplococcus pneumoniae, meningococcus, diphtheria corynebacterium, bacillus pyocyaneus, dysentery bacterium, Bacillus typhi etc. and micrococcus catarrhalis.This product alcohol extract 1:400 can suppress tulase, but decoct 1:100 is invalid.The water decoction of its 1:80 has good killing action to campylobacter pylori.(2) lactogenesis: this product leaf has the obstruction dredging newborn vascular, promotes the effect of lactogenic.(3) antitumor action; (4) choleretic effect: foreign study, these product have choleretic effect, clinical treatment chronic gallbladder spasmolytic and calculosis effective.In addition this product still has diuresis stomach invigorating, the effects such as hypocatharsis.
Cape jasmine fruit is containing iridoid constituents, acrylic component and flavones ingredient; Also gardenoside, all square rafter glycoside, all square rafter glycoside acid, all plain gentiobiose glycoside of square rafter is contained in Fructus Gardeniae peel and seed.Flos Gardeniae is containing Triterpenoid: Fructus Gardeniae flower acid (gradenlic acid) A, B and gardenia acid (gardenic acid) leaf are containing gardenoside, all square rafter glycosides, Fructus Gardeniae aldehyde (cerbinal), MDJ (methyl dihydrojasmonate), acetic acid benzyl lemon imonene), linalool (linalool) etc.; Rhizome contains PEARLITOL 25C, oleanolic acid (oleanolic acid), stigmasterol (sing-masterol).The pharmacological action of Fructus Gardeniae: the compositions such as (1) choleretic effect Fructus Gardeniae and contained iridoid glycoside all have choleretic effect.(2) to the effect of gastrointestinal tract and pancreas; (3) spontaneous activity in mice can be reduced to the effect Fructus Gardeniae alcohol extract of central nervous system, there is sedation, and have synergism with ciclobarbital soluble, can the length of one's sleep be extended, though the convulsions of pentetrazole still can be resisted. the convulsions of strychnine can not be resisted, its mortality rate can be reduced.(4) resisting pathogenic microbes effect Fructus Gardeniae has inhibitory action to staphylococcus aureus, meningococcus, micrococcus catarrhalis etc., and decoct has the effect of killing leptospira and imago of blood fluke, and infusion has inhibitory action to various skin fungus in vitro.
Folium Isatidis is containing indigo glycoside (indican), indigo glycoside is first hydrolyzed to indanol, change into indigo (inidgo) through air oxygen again, again containing the pharmacological action of indirubin (indirubin) Folium Isatidis: (1) resisting pathogenic microbes effect experiment in vitro shows: Folium Isatidis has broad ectrum antibiotic effect. its decoct is to staphylococcus aureus, alpha streptococcus, meningococcus, Diplococcus pneumoniae, micrococcus catarrhalis, Bacillus typhi, escherichia coli, hemophilus influenza, diphtheria corynebacterium and dysentery bacterium all have inhibitory action to a certain degree. and roadside Folium Isatidis decoct all has bactericidal action to multiple dysentery bacterium in vitro, test also shows: it, to the dysentery bacterium of suntomycinum, nitrofural, sulfathiazole, berberine sensitivity or drug resistance, all has good antibacterial action. Folium Isatidis also has inhibitory action to encephalitis b virus, mumps virus, influenza virus. also there is killing action to leptospira.(2) inhibitory action is had to the effect polygonum tinctorium ait. leaf decoct of cardiovascular system to Isolated Toad Heart, and increase with dosage and strengthen, even cause asystole. have dilating effect to rat lower limb vascular, when blood vessel is contraction state, dilating effect is particularly evident.(3) antiinflammatory, refrigeration function polygonum tinctorium ait. leaf decoct 10g/kg gavage to rabbit, can suppress the local skin inflammatory reaction that dimethylbenzene causes, and reduce capillary permeability.5g/kg gavage can make rat formaldehyde swelling of the feet alleviate or disappear acceleration.5 ~ 10g/kg gavage, can make cholera, typhoid fever mixed vaccine causes the rabbit body temperature of heating obviously to decline.
Positive beneficial effect of the present invention:
(1) the present invention utilizes domestic a large amount of Chinese herbal medicine resource, according to the principle of Chinese medical theory and distemper disease, Appropriate application Chinese herbal medicine function cures mainly reasonable compatibility and uses a kind of Chinese medicine compound hydro-acupuncture preparation preventing and treating poultry distemper disease developed, this product is pure Chinese medicinal preparation, good to poultry distemper disease therapeuticing effect, and Chinese herbal medicine can overcome resistance problems and meet the production requirement of non-harmful product.This invention medicine is oral liquid or injection, and application is convenient; Mechanization production, output is large, and cost is low, and sanitary standard also easily reaches.
(2) the present invention has the effect of broad-spectrum antiviral, anti-bacteria, antiinflammatory of bringing down a fever, raising cellular immune function, loose exopathogen, clear endogenous fire, drives the evil poison of pestilence.Some row clinical and experimental study results show also have powerful broad-spectrum disease resistance toxic action.Effectively can prevent and treat the pyretic toxicity disease such as poultry influenza, fowl mild influenza of distemper toxication infringement caused by body.
(3) Chinese herbal and crude drugs preparations of the present invention is to nutrient substance and medicine without adsorption, and not Developing restraint breeding, does not affect curative effect of medication; There are health care and Immunie-Modulating, are conducive to the recovery of affected organ and tissue; Growth promoting effects is bred, and obviously promotes fertility performance, increases economic efficiency.
Detailed description of the invention:
Below in conjunction with embodiment, the present invention is further described, but do not limit content of the present invention.
Embodiment 1:
Prevent and treat a Chinese medicine compound hydro-acupuncture preparation for poultry distemper disease, it is characterized in that being prepared from by the crude drug of following weight portion: Flos Lonicerae 180g, Herba Taraxaci 600g, Fructus Gardeniae 240g and Folium Isatidis 290g.
The preparation method of the Chinese medicine compound hydro-acupuncture preparation of control poultry distemper disease comprises the steps:
(1) pretreatment of crude drug: by the remove impurity respectively of Flos Lonicerae, Herba Taraxaci, Fructus Gardeniae and Folium Isatidis, dry, being ground into granularity is that 40 ~ 160 order fine powders are for subsequent use;
(2) take Flos Lonicerae, Herba Taraxaci, Fructus Gardeniae and Folium Isatidis according to above-mentioned formula, add flooding three times, the first time lixiviate water soaking 30 ~ 40min of 12 times of Flos Lonicerae weight, slow fire boiling 40 hours, filters, obtains the first filtrate; The water that first time filtration medicinal residues add 10 times of Flos Lonicerae weight carries out second time lixiviate, slow fire boiling 30 hours, filters, obtains the second filtrate; The water that second time filtration medicinal residues add 10 times of Flos Lonicerae weight again carries out third time lixiviate, slow fire boiling 30min, filters, obtains the 3rd filtrate, merge the first filtrate, the second filtrate and the 3rd filtrate;
(3) the filtrate centrifugal treating after step (2) being merged obtains supernatant, and supernatant is carried out microfiltration, ultrafiltration, and the filtrate after ultrafiltration is 1.2 ~ 1.36 through being evaporated to relative density;
(4) medicinal liquid adding distil water after step (3) concentrating under reduced pressure is settled to amount of preparation, fill, sterilizing get product.
Embodiment 2:
Prevent and treat a Chinese medicine compound hydro-acupuncture preparation for poultry distemper disease, be prepared from by the crude drug of following weight portion: Flos Lonicerae 150g, Herba Taraxaci 700g, Fructus Gardeniae 250g and Folium Isatidis 250g.
The preparation method of the present embodiment is identical with embodiment 1.
Embodiment 3:
Prevent and treat a Chinese medicine compound hydro-acupuncture preparation for poultry distemper disease, be prepared from by the crude drug of following weight portion: Flos Lonicerae 160g, Herba Taraxaci 650g, Fructus Gardeniae 200g and Folium Isatidis 270g.
The preparation method of the present embodiment is identical with embodiment 1.
Embodiment 4:
Prevent and treat a Chinese medicine compound hydro-acupuncture preparation for poultry distemper disease, be prepared from by the crude drug of following weight portion: Flos Lonicerae 170g, Herba Taraxaci 630g, Fructus Gardeniae 220g and Folium Isatidis 350g.
The preparation method of the present embodiment is identical with embodiment 1.
Embodiment 5:
Prevent and treat a Chinese medicine compound hydro-acupuncture preparation for poultry distemper disease, be prepared from by the crude drug of following weight portion: Flos Lonicerae 180g, Herba Taraxaci 610g, Fructus Gardeniae 240g and Folium Isatidis 260g.
The preparation method of the present embodiment is identical with embodiment 1.
Embodiment 6:
Prevent and treat a Chinese medicine compound hydro-acupuncture preparation for poultry distemper disease, be prepared from by the crude drug of following weight portion: Flos Lonicerae 190 parts, Herba Taraxaci 580g, Fructus Gardeniae 260g and Folium Isatidis 320g.
The preparation method of the present embodiment is identical with embodiment 1.
Embodiment 7:
Prevent and treat a Chinese medicine compound hydro-acupuncture preparation for poultry distemper disease, be prepared from by the crude drug of following weight portion: Flos Lonicerae 200g, Herba Taraxaci 500g, Fructus Gardeniae 300g and Folium Isatidis 300g.
The preparation method of the present embodiment is identical with embodiment 1.
Clinical trial
Materials and methods:
1.1 test material
Chinese medicine compound hydro-acupuncture preparation of the present invention; Amantadine is purchased from Beijing North medicine medication chemistry company.
1.2 test mices are selected and grouping
Test arrangement is in 3 closed mices in different onset stage at certain, adopts pathological material of disease, blood sample cuts open inspection through AGP, HI test, antibacterial culturing, virus purification be diagnosed as pestilence hypotype in conjunction with clinical symptoms, pathology.First group 100 completely randoms are divided into test group 50, matched group 50; Second group 100 completely randoms are divided into test group 50, matched group 50; Third group of 100 completely random is divided into test group 50, matched group 50.3 groups are mice in 28 day age for examination mice.First group mice group is morbidity the 3rd age in days, and second group mice group not yet occurs symptom but is in incubation period, and third group of mice group is the 14th age in days after morbidity.
1.3 feeding and management
Eachly carry out feeding and management by normal feeding and management way, each feeding and management condition is identical, and each test group its corresponding matched group management method is also just the same.
1.4 testing program
The amantadine drinking-water of each matched group 160mg/L, the test group Chinese medicine compound hydro-acupuncture preparation of the embodiment of the present invention 1, test first group dosage is 200mg/L, and test second, third group of dosage are 160mg/L, continuous use 14d.Observe every day and record each group of mice spirit, search for food, drink water, feces, product number, situation such as morbidity number and death toll etc.In this test, second group mice group test the 4th day two groups all start morbidity, the matched group of third group of mice group is the 20th day recurrence pestilence H9 hypotype behind the 6th day of test i.e. onset.
2 results and analysis
Result of the test is in table 1.As can be seen from the test results, during on-test, each test group and matched group yield level are quite (P>0.05), in-test, each test group is obviously better than corresponding matched group in all indexs, each test group compares with corresponding matched group, on sickness rate, the performance of first group is identical, second group performance significant difference (P<0.05), third performance pole significant difference (P<0.01); On mortality rate, not significantly (P>0.05), third group of performance difference extremely significantly (P<0.01) for first, second two group performance difference; In productive rate fall, first, second, the third 3 group performance differences are all extremely significantly (P<0.01); In deformity (soft shell etc.) rate, first, second, the third 3 group performance differences are all significantly (P<0.05).
Table 1 result of the test
3 conclusions
By the result of this controlling experiment in conjunction with the epidemic characteristic of primary disease in locality, these points conclusion can be drawn:
(1) before, during and after pestilence morbidity, 3 stages apply the Chinese medicine compound hydro-acupuncture preparation interpolation control that the present invention prevents and treats poultry distemper disease, all greatly can reduce the mortality rate of mice and the amplitude of productive rate decline, Chinese medicine compound hydro-acupuncture preparation has obvious antiviral, anti-inflammatory effect; Obviously be better than amantadine.
(2) the resistance to mice group excessively of first attack obviously obtains certain immunity to this condition of disease, but because primary disease destroys mouse immune system, many mice groups often have after onset 20 ~ 30d recurs primary disease, to some extent if add compound honeysuckle after onset effectively can prevent this palindromia.
(3) the Chinese medicine compound hydro-acupuncture preparation control primary disease of application control poultry distemper disease is obviously better than amantadine in the decline of reduction productive rate, the recovery of chitin amount.
(4) traditional Chinese medical science thinks the drug effect that Flos Lonicerae has " granulation promoting, consolidates in holding up for invigorating the spleen and replenishing QI, consolidating superficial resistance yang invigorating ".The Chinese medicine compound hydro-acupuncture preparation of control poultry distemper disease is formed by exquisite refine by Flos lonicerae, main component is the panimmunity enhancing substance such as Flos Lonicerae I, Flos Lonicerae II, be equipped with Herba Taraxaci, Fructus Gardeniae and Folium Isatidis again, mutual support, can produce a large amount of LeIF and phagocyte, lymphocyte by excitating organism; And the humoral immunization approach can strengthened in body, produce the white and complement of a large amount of immune balls, improve microcirculation, conditioning and the humoral immunization of enhancing body and cellular immunization effectively, improve body's immunity, virus is produced disturb, engulf, combination, number of ways kill virus and antibacterial, and have effect of heat-clearing and toxic substances removing and adjustment body metabolic disorders, strengthen birds resistance against diseases.
The present invention obtains obvious curative effects in clinical practice, in order to be applied to clinical applicant better according to registration declaration material requirement in " new drug registration management way ", carries out Mouse Acute Toxicity experiment and rat long term toxicity test to said preparation.Result of the test illustrates that the Mouse Acute Toxicity of compound honeysuckle injection is less, points out larger dose application said preparation in clinical a day to produce toxic reaction.Lower to the long-term peroral administration toxicity of rat, safety is better.
The Mouse Acute Toxicity research of the Chinese medicine compound hydro-acupuncture preparation of control poultry distemper disease
The present invention is in Chinese veterinarian's theoretical basis, and in conjunction with the achievement in research of modern Chinese medicine to livestock and poultry virus, refining pure Chinese medicinal preparation, has and act on fast, curative effect high.
The present invention obtains obvious curative effects in clinical practice, in order to be applied to clinical applicant better according to declaration material requirement in " novel chiral synthon Manufacture management way ", carries out Mouse Acute Toxicity experiment and rat long term toxicity test to said preparation.
1, test medicine is tested
Test sample is the injection of control poultry distemper disease, and brownish-yellow powder (100 order), by our company's development and production voluntarily, Clinical practice amount is 330mg/Kg.Preparing test sample concentration with distilled water before experiment is 30% (this concentration is the Cmax that said preparation can complete mouse stomach operation), for experimental applications, is mouse stomach.
2, laboratory animal
ICR strain SPF level mice, male and female totally 40, weight: 18 ~ 21.5g.Be purchased from Medical School of Zhengzhou University Experimental Animal Center, credit number SCXK (Henan) 2009-0001.After mice normally raises 3d, test.
3, experimental program
Trial test display said preparation can not determine median lethal dose(LD 50) (LD
50), therefore, do maximum dosage-feeding and measure.
Experiment grouping: 40 mices are divided into matched group and administration group at random, often organize male
female (♀) each 10.Control group administered physiological saline 0.4ml/10g, 3 times/d; Administration group to test sample 300g/ (kgd) (30%, 0.4ml/10g), 3 times/d.
Administration and experiment condition: normally raise after administration, observe 2 weeks, the body weight of 1,2 week before the mice abnormal response after record administration and administration and after administration.Experimental situation (SPF animal housing) room temperature: (23 ± 1.0) DEG C; Humidity: (46 ± 3) %.Mice empty stomach or half fasted conditions before administration, three delivery time: 1 ~ 2h.
Table 1 body weight statistical table (g)
4, experimental result
Control group mice after gavage gives normal saline in 2 weeks, reaction without exception.Administration group mice is after giving the test sample second day (20h) of compound honeysuckle injection, and defecate is brown, shapeless, fecal pollution anus and crissum.Within 3rd day, play feces character and comprise the recovery such as color, shape normally, until experiment terminates.
In whole experimental period, test sample group (administration group) mice of the injection of control poultry distemper disease, except above-mentioned feces character changes, has no other abnormal responses such as ingesting, drink water, be movable.Matched group and administration group mice are all without dead.
Wherein maximum dosage-feeding: 300g/kg/d (30%).Mice Body mass change is in table 1, increase comparatively matched group after administration group Mice Body mass delivery slow, but between matched group and administration group, male female respectively at the more equal no difference of science of statistics of the body mass ratio of each time point (Using statistics software SPSS130 version).
5, experiment conclusion
The test sample maximum dosage-feeding of the injection of control poultry distemper disease is 500g/ (kgd), and concentration is 50%.Under heavy dose of administration, the rarely seen feces character caused comparatively greatly due to dosage of mice changes and weight increasess slowly, and has no the overt toxicity reaction caused by said preparation, also without dead mouse.
It is less that this result of the test illustrates that the present invention prevents and treats the Mouse Acute Toxicity of the injection of poultry distemper disease, points out larger dose application said preparation in clinical a day to produce toxic reaction.
Claims (5)
1. prevent and treat a Chinese medicine compound hydro-acupuncture preparation for poultry distemper disease, it is characterized in that being prepared from by the crude drug of following weight portion: Flos Lonicerae 15 ~ 20 parts, Herba Taraxaci 50 ~ 70 parts, Fructus Gardeniae 20 ~ 30 parts and Folium Isatidis 25 ~ 35 parts.
2. according to claim 1, prevent and treat the Chinese medicine compound hydro-acupuncture preparation of poultry distemper disease, it is characterized in that being prepared from by the crude drug of following weight portion: Flos Lonicerae 17 ~ 19 parts, Herba Taraxaci 58 ~ 62 parts, Fructus Gardeniae 23 ~ 25 parts and Folium Isatidis 28.5 ~ 31.5 parts.
3. according to claim 1, prevent and treat the Chinese medicine compound hydro-acupuncture preparation of poultry distemper disease, it is characterized in that being prepared from by the crude drug of following weight portion: Flos Lonicerae 18 parts, Herba Taraxaci 60 parts, Fructus Gardeniae 24 parts and Folium Isatidis 29 parts.
4. according to any one of claim 1 ~ 3, prevent and treat the Chinese medicine compound hydro-acupuncture preparation of poultry distemper disease, it is characterized in that described Chinese medicine compound hydro-acupuncture preparation is oral liquid formulation or injection dosage form.
5. described preparation method of preventing and treating the Chinese medicine compound hydro-acupuncture preparation of poultry distemper disease according to any one of claim 1 ~ 3, is characterized in that comprising the steps:
(1) pretreatment of crude drug: by the remove impurity respectively of Flos Lonicerae, Herba Taraxaci, Fructus Gardeniae and Folium Isatidis, dry, being ground into granularity is that 40 ~ 160 order fine powders are for subsequent use;
(2) take Flos Lonicerae, Herba Taraxaci, Fructus Gardeniae and Folium Isatidis according to above-mentioned formula, add flooding three times, the first time lixiviate water soaking 30 ~ 40min of 12 times of Flos Lonicerae weight, slow fire boiling 40 hours, filters, obtains the first filtrate; The water that first time filtration medicinal residues add 10 times of Flos Lonicerae weight carries out second time lixiviate, slow fire boiling 30 hours, filters, obtains the second filtrate; The water that second time filtration medicinal residues add 10 times of Flos Lonicerae weight again carries out third time lixiviate, slow fire boiling 30min, filters, obtains the 3rd filtrate, merge the first filtrate, the second filtrate and the 3rd filtrate;
(3) the filtrate centrifugal treating after step (2) being merged obtains supernatant, and supernatant is carried out microfiltration, ultrafiltration, and the filtrate after ultrafiltration is 1.2 ~ 1.36 through being evaporated to relative density;
(4) medicinal liquid adding distil water after step (3) concentrating under reduced pressure is settled to amount of preparation, fill, sterilizing get product.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104826064A (en) * | 2015-05-18 | 2015-08-12 | 何兴江 | Traditional Chinese medicine preparation for preventing duck plague and preparation method thereof |
| CN107260807A (en) * | 2017-06-14 | 2017-10-20 | 沈阳伟嘉牧业技术有限公司 | A kind of complex injection of Chinese materia medica and preparation method for treating Haemophilus parasuis |
| CN114306554A (en) * | 2021-12-22 | 2022-04-12 | 河南天纳图实业有限公司 | A compound Chinese medicinal preparation for broad-spectrum antibacterial of animals, and its preparation method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1739686A (en) * | 2005-09-29 | 2006-03-01 | 张正生 | Chinese medicine injection for clearing away heat and toxic matter and its prepn process |
| CN103816399A (en) * | 2014-02-24 | 2014-05-28 | 张艳雯 | Traditional Chinese medicinal preparation for treating canine distemper |
| BR102012032588A2 (en) * | 2012-12-20 | 2014-08-26 | Univ Fed Vicosa | PHARMACEUTICAL COMPOSITIONS BASED ON BACCHARIS TRIMERA EXTRACTS AND THE USE OF THESE IN THE PREPARATION OF THERAPEUTIC AGENTS FOR THE PREVENTION AND CONTROL OF CANINE DISCtemper |
| CN104055888A (en) * | 2013-03-21 | 2014-09-24 | 申宏 | Health care composition capable of clearing heat and detoxifying |
-
2014
- 2014-10-28 CN CN201410593739.8A patent/CN104288284A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1739686A (en) * | 2005-09-29 | 2006-03-01 | 张正生 | Chinese medicine injection for clearing away heat and toxic matter and its prepn process |
| BR102012032588A2 (en) * | 2012-12-20 | 2014-08-26 | Univ Fed Vicosa | PHARMACEUTICAL COMPOSITIONS BASED ON BACCHARIS TRIMERA EXTRACTS AND THE USE OF THESE IN THE PREPARATION OF THERAPEUTIC AGENTS FOR THE PREVENTION AND CONTROL OF CANINE DISCtemper |
| CN104055888A (en) * | 2013-03-21 | 2014-09-24 | 申宏 | Health care composition capable of clearing heat and detoxifying |
| CN103816399A (en) * | 2014-02-24 | 2014-05-28 | 张艳雯 | Traditional Chinese medicinal preparation for treating canine distemper |
Non-Patent Citations (2)
| Title |
|---|
| 张永霞等: "清热类中药辅助治疗犬瘟热效果观察", 《中国畜牧兽医文摘》, vol. 28, no. 5, 31 December 2012 (2012-12-31), pages 192 - 163 * |
| 牛建荣等: "中草药配合西药治疗犬瘟热研究进展", 《兽药研究与应用》, no. 3, 31 December 2008 (2008-12-31), pages 31 - 34 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104826064A (en) * | 2015-05-18 | 2015-08-12 | 何兴江 | Traditional Chinese medicine preparation for preventing duck plague and preparation method thereof |
| CN104826064B (en) * | 2015-05-18 | 2018-05-29 | 九江牧威利元科技中心（普通合伙） | A kind of Chinese medicine preparation for preventing duck plague and preparation method thereof |
| CN107260807A (en) * | 2017-06-14 | 2017-10-20 | 沈阳伟嘉牧业技术有限公司 | A kind of complex injection of Chinese materia medica and preparation method for treating Haemophilus parasuis |
| CN114306554A (en) * | 2021-12-22 | 2022-04-12 | 河南天纳图实业有限公司 | A compound Chinese medicinal preparation for broad-spectrum antibacterial of animals, and its preparation method |
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