CN104381592A - Feed for the treatment of respiratory diseases in chickens and preparation method thereof - Google Patents

Abstract

The present invention discloses a feed for the treatment of respiratory diseases in chickens. The feed is composed of traditional Chinese medicine composition and a feed carrier. Compared with 100 parts by weight feed, there are 10-20 parts by weight traditional Chinese medicine composition which consists of the raw materials (parts by weight): 5-15 Scutellaria baicalensis, 4-18 Ephdra vulgaris, 3-10 Perilla frutescens, 5-16 Houttuynia cordata, 3-9 Cortex Phellodendri, 4-12 Forsythia suspensa, 3-14 dandelions, 3-15 Cyrtomium fortunei, 5-20 radix isatidis, 5-17 herba taching, 5-15 liquorice, 1-9 semen lepidii, 1-10 Polygonum cuspidatum and 2-9 Bulbus Fritillariae Cirrhosae; the feed carrier consists of the following raw materials (parts by weight): 2-7 acorn powder, 10-20 wheat brans, 10-30 corn flour, 10-20 soybean cake powder, 5-15 high-gluten flour, 1-3 sodium carbonate, 1-3 sodium bicarbonate, 5-10 fish meal and 1-5 chestnut flour. The feed has an effect in prevention and treatment for chicken infectious respiratory disease, can enhance disease resistance and improve survival rate of chickens, and has advantages of low prices and wide promotional applications.

Description

A kind of feed being used for the treatment of chicken respiratory tract disease and preparation method thereof

 

This case is divisional application, and the denomination of invention of original application is: a kind of Chinese medicine composition being used for the treatment of chicken respiratory tract disease and preparation method thereof, and the applying date of original application is: 2013-09-23, and the application number of original application is: 201310434108.7.

 

Technical field

The present invention relates to the beast medicinal preparation containing deriving from plant, animal or mineral component, particularly a kind of feed being used for the treatment of chicken respiratory tract disease and preparation method thereof.

 

Background technology

The bacterial respiratory diseases of the kind of chicken respiratory tract disease: a..Chronic respiratory tract disease (septic mycoplasmosis), aspergillosis; B. the viral respiratory diseases caused.As ewcastle disease (N D), infective bronchitis (IB), infectious laryngotracheitis (ILT), influenza (AI); C. the respiratory diseases that causes of parasite.As Cryptosporidiosis, leucocyte protozoon disease, endotracheal suction parasitosis.The epidemic characteristic 1. of chicken respiratory tract disease is along with the fast development of poultry husbandry, and the kind of chicken respiratory tract disease gets more and more.The old complaint that presents current chicken respiratory tract disease continues to occur, and the new sick feature constantly occurred, as ewcastle disease, infective bronchitis, infectious laryngotracheitis, Frustrate blood and mycoplasma disease, colibacillosis, infective rhinitis etc., chronic illness is Occurrence & epidemic constantly all the year.Meanwhile, many new sick successively generations, as chicken influenza, Glandular Stomach Type Infections Bronchitis etc.In these emerging epidemic diseases, the prevention and control of chicken influenza epidemic disease to be paid attention at present especially, prevent the Occurrence & epidemic of this disease from being the task of top priority.2. occur cause of disease variation, immunosupress, atypia disease case increase.In recent years, each chicken house is all at the various vaccine immunity of use, but a lot of plain vaccine is more and more difficult at some aspect of respiratory disease of prevention and control at present, causes the chicken group after immunity not necessarily all can produce strong protection.Chicken will be made to mass-send when running into external environment and having wild poison to pollute sick.3. the harm of some bacteriosis and parasitic disease is on the rise.Along with increasing and the continuous expansion of scale of feeding chicken in largely scale field, environmental pollution is also more and more serious.Bacteriosis and parasitic disease showed increased, wherein many cause of diseases are extensively present in poultry environment, and propagate by number of ways, these pathogenic microorganisms have become the cause of disease of being everlasting in chicken farm.More chief reason is poultry family blindly heavy dose of Domain of Abuse Antibiotics, makes some common bacterias produce stronger drug resistance, once after morbidity, all drug are all difficult to prove effective.4. the case of mixed infection and scabies secondary infection increases.In production practices, along with epidemic disease increase, cultivation density strengthen, add the factors such as environment disinfected is not tight, precautionary measures are ineffective, single cause of disease is rare clinically in chicken disease, and a lot of case is by two or more cause of disease, produce pathogenic effects to same chicken body, the case of accompanying infection, scabies secondary infection and mixed infection rises.

At present due to the complexity of chicken respiratory disease, therefore specific medicament is not used for treating, and Chinese medicine is in line with the illness of principle for above-mentioned complexity consolidating righting, there is fine therapeutic action and application prospect, the invention provides a kind of Chinese medicine composition for the treatment of chicken respiratory tract disease, effectively treat chicken respiratory disease, add survival rate.

Summary of the invention

The object of the present invention is to provide a kind of instant effect and the little Chinese medicine composition being used for the treatment of chicken respiratory tract disease of side effect and preparation method thereof.

In order to realize foregoing invention object, the invention provides a kind of Chinese medicine composition being used for the treatment of chicken respiratory tract disease, in described Chinese medicine composition, the ratio of weight and number of each raw medicinal material is: root of large-flowered skullcap 5-15 part, Chinese ephedra 3-18 part, purple perilla 3-10 part, cordate houttuynia 4-17 part, golden cypress 2-10 part, capsule of weeping forsythia 3-14 part, dandelion 2-14 part, rhizome of cyrtomium 2-17 part, Radix Isatidis 3-22 part, folium isatidis 3-18 part and Radix Glycyrrhizae 4-16 part.

Described Chinese medicine composition also comprises: lepidium seed, giant knotweed and Bulbus Fritillariae Cirrhosae; In described Chinese medicine composition, the ratio of weight and number of each raw medicinal material is: root of large-flowered skullcap 5-15 part, Chinese ephedra 4-18 part, purple perilla 3-10 part, cordate houttuynia 5-16 part, golden cypress 3-9 part, capsule of weeping forsythia 4-12 part, dandelion 3-14 part, rhizome of cyrtomium 3-15 part, Radix Isatidis 5-20 part, folium isatidis 5-17 part, Radix Glycyrrhizae 5-15 part, lepidium seed 1-9 part, giant knotweed 1-10 part and Bulbus Fritillariae Cirrhosae 2-9 part.

In described Chinese medicine composition, the ratio of weight and number of each raw medicinal material is preferably: root of large-flowered skullcap 8-15 part, Chinese ephedra 4-12 part, purple perilla 5-10 part, cordate houttuynia 5-12 part, golden cypress 3-6 part, capsule of weeping forsythia 4-7 part, dandelion 8-14 part, rhizome of cyrtomium 9-15 part, Radix Isatidis 11-20 part, folium isatidis 9-17 part, Radix Glycyrrhizae 5-11 part, lepidium seed 1-5 part, giant knotweed 6-10 part and Bulbus Fritillariae Cirrhosae 5-9 part.

In described Chinese medicine composition, the ratio of weight and number of each raw medicinal material can also be preferably: root of large-flowered skullcap 5-11 part, Chinese ephedra 9-18 part, purple perilla 3-8 part, cordate houttuynia 8-16 part, golden cypress 5-9 part, capsule of weeping forsythia 6-12 part, dandelion 3-11 part, rhizome of cyrtomium 3-11 part, Radix Isatidis 5-14 part, folium isatidis 5-11 part, Radix Glycyrrhizae 8-15 part, lepidium seed 4-9 part, giant knotweed 1-7 part and Bulbus Fritillariae Cirrhosae 2-7 part.

In described Chinese medicine composition, the ratio of weight and number of each raw medicinal material can be preferably again: root of large-flowered skullcap 7-13 part, Chinese ephedra 6-16 part, purple perilla 4-8 part, cordate houttuynia 7-13 part, golden cypress 3-9 part, capsule of weeping forsythia 4-12 part, dandelion 3-12 part, rhizome of cyrtomium 3-15 part, Radix Isatidis 5-20 part, folium isatidis 5-15 part, Radix Glycyrrhizae 5-15 part, lepidium seed 3-7 part, giant knotweed 3-6 part and Bulbus Fritillariae Cirrhosae 4-7 part.

In described Chinese medicine composition, the ratio of weight and number of each raw medicinal material can also be preferably: the root of large-flowered skullcap 10 parts, 12 parts, Chinese ephedra, purple perilla 6 parts, cordate houttuynia 10 parts, golden cypress 6 parts, the capsule of weeping forsythia 8 parts, dandelion 7 parts, rhizome of cyrtomium 9 parts, Radix Isatidis 12 parts, folium isatidis 10 parts, 10 parts, Radix Glycyrrhizae, lepidium seed 5 parts, giant knotweed 5 parts and Bulbus Fritillariae Cirrhosae 5 parts.

In order to better realize foregoing invention object, the invention provides a kind of preparation method being used for the treatment of the Chinese medicine composition of chicken respiratory tract disease, the formulation of described Chinese medicine composition is pulvis, and described preparation method comprises the following steps:

(1) take all raw medicinal materials according to ratio of weight and number, after pulverizing, drop into mixer and stir 40-60 minute, fully mix to obtain mixture;

(2) in mixture, add the water relative to mixture quality 2 ~ 4 times, decoct 2 ~ 4 hours, filter, in filter residue, add the water relative to 1 ~ 2 times, mixture, again decoct 1 ~ 2 hour, filter, by twice filtrate mixing, reduced pressure concentration removing moisture, obtain dry lotion, dry with spray dryer further and pulverize, sieve acquisition 50 ~ 100 order fine powder, obtains pulvis.

The formulation of described Chinese medicine composition is decoction, and its preparation method comprises the following steps:

(1) take all raw medicinal materials, mix rear pulverizing, obtain mixture;

(2) in the mixture after pulverizing, add the water relative to described mixture quality 2 ~ 4 times, decoct 2 ~ 4 hours, filter, add in filter residue and again decoct 1 ~ 2 hour relative to the water of 1 ~ 2 times, described mixture, filter, by twice filtrate mixing, being evaporated to crude drug concentration is 1g/ml ~ 3g/ml.

Present invention also offers a kind of feed, described feed comprises feed addictive and feed vector, and relative to the described feed of 100 weight portions, the addition of described Chinese medicine composition is 10-20 weight portion.

Described feed vector is made up of the raw material of following ratio of weight and number: acorn nut powder 2-7 part, wheat bran 10-20 part, corn flour 10-30 part, beancake powder 10-20 part, Strong flour 5-15 part, sodium carbonate 1-3 part, sodium acid carbonate 1-3 part, fish meal 5-10 part and rice-chestnut powder 1-5 part.

Described feed vector is preferably made up of the raw material of following ratio of weight and number: 4.5 parts, acorn nut powder, wheat bran 15 parts, corn flour 20 parts, beancake powder 15 parts, Strong flour 10 parts, 2 parts, sodium carbonate, sodium acid carbonate 2 parts, fish meal 7 parts and rice-chestnut powder 3 parts.

The preparation method of described feed comprises the following steps:

(1) take all raw medicinal materials according to ratio of weight and number, after pulverizing, drop into mixer and stir 40-60 minute, fully mix to obtain mixture; The water relative to mixture quality 2 ~ 4 times is added in mixture, decoct 2 ~ 4 hours, filter, in filter residue, add the water relative to 1 ~ 2 times, mixture, again decoct 1 ~ 2 hour, filter, by twice filtrate mixing, reduced pressure concentration removing moisture, obtain dry lotion, dry with spray dryer further and pulverize, sieve acquisition 50 ~ 100 order fine powder, obtains pulvis;

(2) raw material is taken according to following ratio of weight and number: 4.5 parts, acorn nut powder, wheat bran 15 parts, corn flour 20 parts, beancake powder 15 parts, Strong flour 10 parts, 2 parts, sodium carbonate, sodium acid carbonate 2 parts, fish meal 7 parts and rice-chestnut powder 3 parts, add water Strong flour, sodium carbonate and sodium acid carbonate modulation, refining temperature is 55-60 DEG C, pour into after stirring in spraying machine and dry, pulverize acquisition fine powder;

(3) pulverize after the mixing of acorn nut powder, wheat bran, corn flour, beancake powder, fish meal and rice-chestnut powder, grinding particle size is 350-600mm, and make fine powder amount reach more than 95% of total material, the fine powder obtained with step (2) mixes, and obtains feed vector;

(4) pulvis step (1) obtained and step (3) obtain feed vector mixing; put into multiplex mixer to stir 60 minutes ~ 110 minutes; ring mode bulking machine is put into expanded after taking-up; control the temperature of bulking machine between 80 DEG C ~ 95 DEG C; the expanded time is 1 ~ 2 hour, and the aridity controlling material to be extruded is 90% ~ 95%, proceeds in ring mode grit machine and be produced into precipitation hard particles feed after taking-up; then sieve by 10 meshes, obtain described feed.

 

The beneficial effect that technical scheme provided by the invention is brought is: feed of the present invention has the effect of prevention and therapy to avian infectious breathing problem, can strengthen chicken premunition and improve chicken survival rate, cheap, have advantages such as applying.

 

Detailed description of the invention

 

Embodiment 1 pulvis

The embodiment of the present invention 1 provides a kind of Chinese medicine composition being used for the treatment of chicken respiratory tract disease, and formulation is pulvis, and preparation method comprises the following steps:

(1) root of large-flowered skullcap 1000g, Chinese ephedra 1200g, purple perilla 600g, cordate houttuynia 1000g, golden cypress 600g, capsule of weeping forsythia 800g, dandelion 700g, rhizome of cyrtomium 900g, Radix Isatidis 1200g, folium isatidis 1000g, Radix Glycyrrhizae 1000g, lepidium seed 500g, giant knotweed 500g and Bulbus Fritillariae Cirrhosae 500g is taken, after pulverizing, drop into mixer and stir 60 minutes, fully mix to obtain mixture;

(2) in mixture, add the water relative to mixture quality 3 times, decoct 3 hours, filter, in filter residue, add the water relative to 2 times, mixture, again decoct 2 hours, filter, by twice filtrate mixing, reduced pressure concentration removing moisture, obtain dry lotion, dry with spray dryer further and pulverize, sieve acquisition 80 order fine powder, obtains pulvis.

 

Embodiment 2 pulvis

The embodiment of the present invention 2 provides a kind of Chinese medicine composition being used for the treatment of chicken respiratory tract disease, formulation is pulvis, preparation method comprises the following steps: take root of large-flowered skullcap 1000g, Chinese ephedra 1100g, purple perilla 700g, cordate houttuynia 1100g, golden cypress 600g, capsule of weeping forsythia 600g, dandelion 700g, rhizome of cyrtomium 900g, Radix Isatidis 1300g, folium isatidis 1100g and Radix Glycyrrhizae 1000g, after pulverizing, drop into mixer and stir 55 minutes, fully mix to obtain mixture;

(2) in mixture, add the water relative to mixture quality 4 times, decoct 2 hours, filter, in filter residue, add the water relative to 2 times, mixture, again decoct 2 hours, filter, by twice filtrate mixing, reduced pressure concentration removing moisture, obtain dry lotion, dry with spray dryer further and pulverize, sieve acquisition 80 order fine powder, obtains pulvis.

 

Embodiment 3 decoction

The embodiment of the present invention 3 provides a kind of Chinese medicine composition being used for the treatment of chicken respiratory tract disease, and formulation is decoction, and its preparation method comprises the following steps:

(1) root of large-flowered skullcap 1200g, Chinese ephedra 800g, purple perilla 700g, cordate houttuynia 800g, golden cypress 500g, capsule of weeping forsythia 500g, dandelion 1100g, rhizome of cyrtomium 1200g, Radix Isatidis 1600g, folium isatidis 1300g, Radix Glycyrrhizae 800g, lepidium seed 300g, giant knotweed 800g and Bulbus Fritillariae Cirrhosae 700g is taken, mix rear pulverizing, obtain mixture;

(2) in the mixture after pulverizing, add the water relative to described mixture quality 3 times, decoct 3 hours, filter, add the water relative to 1 times, described mixture in filter residue, again decoct 1 hour, filter, by twice filtrate mixing, being evaporated to crude drug concentration is 2g/ml.

 

Embodiment 4 feed

The embodiment of the present invention 4 provides a kind of feed being used for the treatment of chicken respiratory tract disease, by percentage by weight be 85% feed vector and percentage by weight be 15% Chinese medicine composition form, wherein Chinese medicine composition is made up of the raw medicinal material of following ratio of weight and number: the root of large-flowered skullcap 12 parts, 8 parts, Chinese ephedra, purple perilla 7 parts, cordate houttuynia 8 parts, golden cypress 6 parts, the capsule of weeping forsythia 6 parts, dandelion 11 parts, rhizome of cyrtomium 12 parts, Radix Isatidis 15 parts, folium isatidis 13 parts, 8 parts, Radix Glycyrrhizae, lepidium seed 3 parts, giant knotweed 8 parts and Bulbus Fritillariae Cirrhosae 7 parts.

The preparation method of Chinese medicine composition comprises the following steps:

(1) root of large-flowered skullcap 1200g, Chinese ephedra 800g, purple perilla 700g, cordate houttuynia 800g, golden cypress 600g, capsule of weeping forsythia 600g, dandelion 1100g, rhizome of cyrtomium 1200g, Radix Isatidis 1500g, folium isatidis 1300g, Radix Glycyrrhizae 800g, lepidium seed 300g, giant knotweed 800g and Bulbus Fritillariae Cirrhosae 700g is taken, after pulverizing, drop into mixer and stir 55 minutes, fully mix to obtain mixture;

(2) in mixture, add the water relative to mixture quality 4 times, decoct 4 hours, filter, in filter residue, add the water relative to 1 times, mixture, again decoct 1.5 hours, filter, by twice filtrate mixing, reduced pressure concentration removing moisture, obtain dry lotion, dry with spray dryer further and pulverize, sieve acquisition 50 order fine powder, obtains pulvis.

The preparation method of feed comprises the following steps:

(1) Chinese medicine powder is prepared according to the method described above;

(2) raw material is taken according to following ratio of weight and number: 4.5 parts, acorn nut powder, wheat bran 15 parts, corn flour 20 parts, beancake powder 15 parts, Strong flour 10 parts, 2 parts, sodium carbonate, sodium acid carbonate 2 parts, fish meal 7 parts and rice-chestnut powder 3 parts, add water Strong flour, sodium carbonate and sodium acid carbonate modulation, refining temperature is 55-60 DEG C, pour into after stirring in spraying machine and dry, pulverize acquisition fine powder;

(3) pulverize after the mixing of acorn nut powder, wheat bran, corn flour, beancake powder, fish meal and rice-chestnut powder, grinding particle size is 350-600mm, and make fine powder amount reach more than 95% of total material, the fine powder obtained with step (2) mixes, and obtains feed vector;

(4) pulvis step (1) obtained and step (3) obtain feed vector mixing; put into multiplex mixer to stir 60 minutes ~ 110 minutes; ring mode bulking machine is put into expanded after taking-up; control the temperature of bulking machine between 80 DEG C ~ 95 DEG C; the expanded time is 1 ~ 2 hour, and the aridity controlling material to be extruded is 90% ~ 95%, proceeds in ring mode grit machine and be produced into precipitation hard particles feed after taking-up; then sieve by 10 meshes, obtain described feed.

 

Wherein, the pharmacology of each raw medicinal material is as follows:

The root of large-flowered skullcap: have heat-clearing and damp-drying drug, cool blood is antiabortive, detoxicating functions.Cure mainly warm heat disease, the infection of the upper respiratory tract, cough with lung heat, damp and hot jaundice, pneumonia, dysentery, hemoptysis, hot eyes, fetal irritability, hypertension, carbuncle swell the diseases such as furuncle sore.The clinical practice of the root of large-flowered skullcap is antibacterial not worse than the coptis, and does not develop immunity to drugs.We are by the strong feature of broad-spectrum antibacterial action, are used in fungal culture miscellaneous bacteria and infect special severe, fine by root of large-flowered skullcap extract effect." Bencao Jingshu ": the root of large-flowered skullcap, its property is respectful clearly, so evil removing: bitter is so eliminating dampness; Cold sensation of the genitalia is so victory warm, therefore main all heat.All hot persons, heat symptoms caused by an exopathgen with all over heat also, jaundice, intestines are out-of-the-way, let out dysentery, and the disease of all damp and hot victory also, analyses it, then all diseases are from recover.Bitter cold can except damp and hot, thus small intestine profit and water from by, source is clearly then flowed also clean.The blood person of closing, real heat is at blood system, and instant heating human blood room, make us obstructed through closing, damp and hot solution, then Ying Qi clearly and voluntarily also.Dislike sore subcutaneous ulcer erosion person, blood-head then stays knot, and is that the carbuncle stain that swells is also rotten: fiery ulcer person, also, cool blood heat extraction, then self-healing also for internal heat blood trouble.

Chinese ephedra: pungent, micro-hardship, temperature.Enter lung, urinary bladder channel.Function with cure mainly: Pulvis Diaphoreticus tremble with fear, freeing lung and relieving asthma, inducing diuresis for removing edema.For anemofrigid cold, uncomfortable in chestly to breath with cough, geomantic omen edema; Bronchial astehma.Herba ephedrae moistens the lung and relieve the cough.Be used for table disease to separate, asthma and cough.Pharmacological research: sweating: 1. active ingredient volatile oil, ephedrine.2. condition warmly take, thermal condition.3. the mechanism of action: hinder sudoriferous duct to the heavily absorption → sweat gland secretion of sodium ion ↑; Excited maincenter, peripheral alpha acceptor.Relieving asthma: 1. active ingredient ephedrine, d-pseudo-ephedrine, volatile oil, in addition, Tetramethylpyradine L-α-terpinenol.2. mechanism: the release promoting NA and Adr.Exciting β-R → activation Ac → promotion cAMP itself produces.Stop the release of Anaphylactic mediator.Promote the release of PGE.Direct excited alpha-2-adrenoceptor → vessel retraction → mucous membrane swelling alleviates.Antibody is suppressed to produce.3. action character is slow, gentle, lasting, can be oral.Diuresis: 1. active ingredient d-pseudo-ephedrine.2. act on: urine volume increases.3. mechanism: nephrectasia blood vessel → increase renal blood flow.Hinder renal tubule to the heavily absorption of sodium ion.Anti-inflammatory, antiallergy: 1. active ingredient pseudoephedrine, methylephedrine, ephedrine.2. the mechanism of action: suppress irritated tramsmitter release; Hemolysin reduces, in anticomplementary action.Antibechic, to eliminate the phlegm: 1. action intensity is 1/20 of codeine.2. active ingredient terpinenol, ephedra volatile oil.Antipyretic, antibacterial, antiviral: 1. active ingredient volatile oil.2. antibacterial staphylococcus aureus, first, beta hemolysis streptococcus, haemophilus influenzae, Diplococcus pneumopniae, bacillus anthracis, corynebacterium diphtheriae, Escherichia coli, neisser's diplococcus.3. antiviral influenza virus.

Purple perilla: pungent, temperature; Return lung, the spleen channel.There is inducing sweat and dispelling exogenous evils, regulating the flow of QI to ease the stomach, effect of solving toxin of fish and crab.For anemofrigid cold, headache, cough, fullness and oppression of chest and abdomen, fish crab is poisoning." book on Chinese herbal medicine converge with speech ": purple perilla, loose cold air, clearing lung-heat gas, wide middle gas, antiabortive gas, lower knot gas, reduce phlegm gas, be control gas refreshing medicine also.One thing has three with here: as cold typhoid fever, headache ostalgia, fever with aversion to cold, and podomere is unfavorable, or beriberi hernia, evil strongly fragrant table person, and Su Ye can fall apart heresy and inducing sweat; Obstruction of the circulation of vital energy knot and in full ruffian plug, chest is unfavorable, or nausea is forced, abdomen side of body distending pain person, caulis perllae can pleasant and wide in; If or upper asthma is inverse, perillaseed can Dingchuan and lower gas, and convulsive seizure due to phlegm-fire is run quickly and compeled, and fire and clear phlegm can fall in perillaseed, and three see difference used, method when in detail it.The water decoction with antibacterial effect perilla leaf has inhibitory action to staphylococcus aureus; To all unrestraint effects such as Pseudomonas aeruginosa, typhoid bacillus, Escherichia coli, paracolon, S. sonnei and bacterium flexneri, bacillus anthracis etc. perilla leaf medicinal extract has bacteriostasis to fungi, wherein contained perillaldehyde, citral play main bacteriostasis, both also have mutual synergy, this is because two kinds of compounds are all monoterpene system aldehyde materials, its site of action is also similar.The mould restraint of perilla herb oil to inoculation and natural pollution is obviously better than ethylparaben.

Cordate houttuynia: taste is pungent, cold nature, enters lung, urinary bladder channel, has clearing heat and detoxicating, removing pus and relieving carbuncle, effect of inducing diuresis for treating strangurtia, and for lung carbuncle pyemesis, phlegm heat is coughed and breathed heavily, toxic-heat and blood stasis, damp-heat gonorrhoea, hot carbuncle carbuncle, and snakeworm wants first-class card.

Golden cypress: heat-clearing and damp-drying drug, purging intense heat removes steams, detoxification sore treatment.For damp-heat dysentery, jaundice, under band, heat is drenched, beriberi, and impotence is warded off, hectic fever due to yin labor heat, night sweat, seminal emission, sore swollen toxin, eczema itch.Stir-baked CORTEX PHELLODENDRI with salt solution is nourishing Yin and falling fire.For fire excess from yin deficiency, night sweat hectic fever due to yin." Chinese Pharmacopoeia " carries the power of golden cypress heat-clearing and damp-drying drug, similar to the root of large-flowered skullcap, the coptis, but being good except the damp and hot of the part of the body cavity below the umbilicus, housing the bladder, kidneys and bowels.

The capsule of weeping forsythia: bitter, is slightly cold.Return lung, the heart, gallbladder channel.The capsule of weeping forsythia has broad-spectrum antibacterial action, has very strong inhibitory action to staphylococcus aureus, Hayes shigella dysenteriae, has certain inhibitory action to other pathogenic bacteria, influenza virus, fungi.There is antiinflammatory action.Oleanolic acid contained by this product has cardiac stimulant, diuresis and hypotensive activity, and citrin can reduce vasopermeability and fragility, prevents haemolysis.Decoction has the town effect of telling, anti-liver injury effect.Clearing heat and detoxicating, dispersing swelling and dissipating binds, dispelling wind and heat from the body.Cure mainly carbuncle sore tumefacting virus, subcutaneous nodule scrofula, affection of exogenous wind-heat, warm disease from the beginning of, heat drenches puckery pain etc.Control warm, erysipelas, macula, large carbuncle pyogenic infections, scrofula, urine leaching closes, clearing away the heart-fire.1. " herbal classic ": main fever and chills, mouse impotence, scrofula, carbuncle is swollen dislikes sore, goiter and tumor, knot heat.2. " not Lu ": go white worm.3. " property of medicine opinion ": main tonneau five drenches, urinary obstruction, except heart man visitor heat.4. " Japan hanako materia medica ": logical small intestine, apocenosis.Control sore furuncle, pain relieving, logical menstruation.5. Li Gao: loose all menses knot gas gathers; Detumescence.6. Wang Haogu: control deaf Hun Hun Jun Jun.

Dandelion: the effect with resisting pathogenic microbes, Dandelion Injectable Solution has stronger bactericidal action to staphylococcus aureus resistance bacterial strain, hemolytic streptococcus in vitro, also has certain bactericidal action to Diplococcus pneumopniae, meningococcus, corynebacterium diphtheriae, Pseudomonas aeruginosa, proteus, shigella dysenteriae, typhoid bacillus etc. and micrococcus catarrhalis.Dandelion extract (1:400) can suppress tulase in vitro.Dandelion water decoction (1:80) can delay ECHO11 viral cytopathic.Taraxol extract 31mg/kg can kill Leptospira.Water logging agent has inhibitory action to various skin fungi.Decoction, to Oral Administration in Rats, absorbs good, can keep certain antibacterial action in urine.Also have protect the liver, the effect of cholagogic, by dandelion decoction gavage or with Dandelion Injectable Solution injection, the glutamic-pyruvic transaminase caused carbon tetrachloride raises obvious inhibitory action; Significantly can alleviate the Histological change that carbon tetrachloride hepatic injury causes.Have report to point out, dandelion has choleretic effect in animals.Herba Taraxaci liquid gavage can make gallbladder contraction, and oddi's sphincter relaxes, and is conducive to bile and enters in intestines.Clinically to chronic gallbladder spasmolytic and calculosis effective.Also there is immunologic function, dandelion decoction can significantly improve PBLC blast transformation rate in vitro, Taraxacum Polysaccharides significantly can strengthen ehrlich carcinoma and MM46 tumor-cell antigen induced mice foot pad delayed allergy intensity, and have administration time more effective feature more backward, the administration on the 11st ~ 20th namely after antigenic stimulus or 2 ~ 20 the next day administration have obvious effect.Taraxacum Polysaccharides lumbar injection significantly can strengthen the CDCC of mouse antibodies dependence macrophage.

Rhizome of cyrtomium: clearing heat and detoxicating, cooling blood and hemostasis, desinsection cures mainly 1, anemopyretic cold, febrile virulent maculae.This product bitter cold, can the gas reality heat of dividing clearly, has the heat poison separating blood system, and the evil caused card of all warm poison all with it, often can be equal to use with the coptis, folium isatidis, Radix Glycyrrhizae, as rhizome of cyrtomium falls apart (" Prescriptions for Universal Relief ").Single product or join mulberry leaf, honeysuckle etc. and can prevent and treat anemopyretic cold; If with compatibilities such as Radix Isatidis, folium isatidis, Asian puccoons, can be used for again the diseases such as mumps, febrile virulent maculae, dermexanthesis.2, blood-head is hemorrhage.This product bitter is slightly cold, main enter Liver Channel, have the merit of cooling blood and hemostasis, cure mainly the caused bleeding from five sense organs or subcutaneous tissue of blood-head, spit blood, have blood in stool, the disease such as uterine bleeding, be especially apt to control metrostaxis.As " Bencao Tujing " controls bleeding from five sense organs or subcutaneous tissue, can grind into powder and be taken after mixing with liquid by single; If associate with the coptis, grind sticky rice drink into powder and be taken after mixing with liquid, can haematemesis be controlled, as rhizome of cyrtomium loose (" General Records of Holy Universal Relief "); Treating hematochezia can compatibility cacumen biotae; Controlling metrostaxis can with excrementum pteropi with using.3, worm disease.This product has the merit of desinsection.For multiple Enterozoas such as destroying and expelling tenia, hookworm, pinworm, roundworms.Can use with anthelmintic compatibility.4, this product also can be used for treatment burn and scald and married woman be with inferior disease.

Radix Isatidis: resisting pathogenic microbes effect: Radix Isatidis has inhibitory action to various bacteria.Infusion has inhibitory action in various degree to hay bacillus, staphylococcus aureus, sarcine, Escherichia coli, typhoid bacillus, paratyphosum Bacterium, dysentery (will congratulate, Fu Shi) bacillus, bacillus enteritidis etc.; Acetone leachate also has similar effect, and to hemolytic streptococcus effectively (all using agar aperture flat band method).Similar to the bacteriostasis of A type meningococcus and garlic, honeysuckle. infected by influenza PR8 strain and Jing Ke 68-1 strain have obvious inhibitory action.In vitro leptospiral effect killed by Radix Isatidis.To cardiovascular effect: these product can make the in vitro rabbit ear and rat lower limb perfusion flow increase, and improve Rabbit Mesentery microcirculation, reduce the blood pressure of Anaesthetic Rabbits, reduce capillary permeability, and make mouse cardiac muscle oxygen demand decline.Antiaggregating activity on platelet effect Radix Isatidis has remarkable inhibitory action to the Platelet Aggregation in Rabbits that ADP induces.From Radix Isatidis, get uridine, hypoxanthine, pyrimidine, salicylic acid etc. also all show certain inhibit activities to the Platelet Aggregation in Rabbits that ATP induces, when 0.21mg/kg concentration, its inhibiting rate is respectively 18.2%, 24.2%, 10.8% and 20%.Detoxication: dog Radix Isatidis, coptis powder and black false hellebore, with taking (each 2.0g/kg), can separate black false hellebore poison, reduces the death rate; If again with it after veratrum poisoning, then invalid; Alone Radix Isatidis powder or coptis powder respectively, effect is also bad." book on Chinese herbal medicine is stated ": bitter, cold, nontoxic." book on Chinese herbal medicine is just read ": enter liver, stomach blood system." China's book on Chinese herbal medicine ": return liver; Stomach warp.There is heat-clearing, removing toxic substances, cool blood, relieve sore throat effect.Treatment febrile virulent maculae; High heat headache; Major part pestilence; Tongue is secretly dark reddish purple, scarlet fever; Erysipelas; Mumps; Larynx numbness; Sore is swollen, carbuncle swells; Varicella; Measles; Hepatitis; Influenza, epidemic meningitis, encephalitis, pneumonia, coma tells nosebleed, and pharynx is swollen, pinkeye, sore rash; Japanese Type-B encephalitis, acute, chronic hepatitis, mumps, osteomyelitis can be prevented and treated.

Folium isatidis: be mainly used in heat poison and send out the diseases such as spot, erysipelas, abscess of throat, aphthae, sore and toxic.This medicine extensive use clinically in recent years, controls except above-mentioned all diseases except available, can be used for again the strongly fragrant lung of phlegm heat, Huang of coughing up phlegm is thick; Especially be usually used in popular second encephalitis, both can be applied to prevention by single, and radix bupleuri, honeysuckle flower, the capsule of weeping forsythia, Radix Isatidis, radix scrophulariae, the dried rhizome of rehmannia etc. can be coordinated again, can removing summer-heat gas divide, the heat of seek point is malicious, availablely control various encephalitis, and comparatively suitable with partial heat type." detailed outline ": " main pyrotoxic dysentery, jaundice, larynx numbness, erysipelas." " Lan Yezhi separates Chinese blister beetle, Yuan green grass or young crops, tree of heaven chicken, cinnabar, arsenolite poison." " book on Chinese herbal medicine is just ": " control the hot poison of pestilence to go mad, wind-heat macula, large carbuncle swells and ache, relieving thirst and restlessness, only nosebleed epistaxis, haematemesis, kills infantile malnutrition due to digestive disturbances or intestinalparasites erosion, metal-inflicted wound curare.Allly to hold concurrently malicious person with heat, all should smash juice and use it by blue leaf.”

Radix Glycyrrhizae: property is put down, and taste is sweet, returns 12 warps.Invigorate the spleen and benefit qi, clearing heat and detoxicating, expelling phlegm and arresting coughing, relieving spasm to stop pain, coordinating the drug actions of a prescription.For weakness of the spleen and the stomach, lassitude hypodynamia, palpitation, coughing with a lot of sputum, the anxious pain of gastral cavity abdomen, four limbs contraction, carbuncle sore tumefacting virus, cushion toxicity, strongly have removing toxic substances, eliminate the phlegm, pain relieving, spasmolysis so that the pharmacological action such as anticancer, Radix Glycyrrhizae has reducing blood lipid, oxidation resistant effect.

Lepidium seed: pungent; Bitter; Cold.Return lung channel; The heart channel of Hang-Shaoyin; Liver Channel; Stomach warp; Urinary bladder channel.Function: purging the lung of pathogenic fire sending down abnormally ascending; Eliminating phlegm and relieving asthma; Inducing diuresis for removing edema; Let out by heresy.Cure mainly: sputum stops up the dyspnea and cough with excessive sputum of lung; Lung carbuncle; Oedema; Chest abdomen ponding; Difficult urination; Chronic cor pulmonale; Breathing heavily of heart failure is swollen; Scrofula tuberculosis.

Giant knotweed: micro-hardship, is slightly cold.Return liver, courage, lung channel.Function is clearing heat and detoxicating, normalizing gallbladder to cure jaundice, expelling wind and removing dampness, loose stasis of blood analgesic therapy, relieving cough and reducing sputum.For joint pain, jaundice with damp-heat pathogen, through Bi ， wei lump in the abdomen, coughing with a lot of sputum, scald, injury from falling down, carbuncle sore tumefacting virus." medical center bun will " carries it: hard kidney, and strong Yang yi Jing, strengthens the bone, and increases strength.Apply to get injured by a fall and lose place, can reunion of fractured tendons and bones.

Bulbus Fritillariae Cirrhosae: cool in nature, Gan Ping.Enter lung channel, stomach warp.Effect: moisten the lung and relieve the cough resolving sputum and relieving asthma, clearing heat and eliminating phlegm.1. effect (1) the antitussive effect Bulbus Fritillariae Cirrhosae of pair respiratory system has antitussive effect.Employing ammonia induced cough in mice is tested, tissue cultures tendril-leaved fritillary bulb and wild tendril-leaved fritillary bulb, 3.0g/kg gavage, and when 30 minutes, antitussive effect is the strongest, and after cough-relieving rate is greater than 50%, 1 hour, effect starts to decline, until within 2 hours, still have remarkable effect; By 1.5g/kg and 3.0g/kg administration, cough suppressing effect strengthens with dosage and strengthens.Tissue cultures tendril-leaved fritillary bulb and commodity tendril-leaved fritillary bulb have similar relieving cough and reducing sputum effect.Bulbus Fritillariae Cirrhosae TA and non-alkaloid part, oral administration, every mouse 0.25 ~ 0.5mg, all has obvious antibechic effect.The TA that proposes and bulb of fritillary saponin I in the Fritillaria unibracteata of and cultivation wild from North-West Sichuan area, II, No. III, adopt mouse sulfur dioxide to draw and coughs method experiment, alkaloid has no antitussive effect; Bulb of fritillary saponin No. II 0.2g/kg gavage, obviously can extend the incubation period of mouse cough, performance antibechic effect.Scholar is separately had to mix intraperitoneal anesthesia with yellow Jackets 15mg/kg and sodium phenobarbital 75mg/kg to cat, electro photoluminescence superior laryngeal nerve draws typical and stable Respiratory frequency, through the alcohol extract 4g/kg (crude drug) of lumbar injection Anhui shellfish, pressed old terms stimulated every 20 minutes.Result shows, and the suppression that after medication, the cough intensity of cat and cough number of times are all subject in various degree, produces significant antitussive effect in 60 minutes, continue 2 ~ 3 hours.Shellfish is compared with tendril-leaved fritillary bulb in Anhui, and both antitussive effects are without significant difference; And Anhui shellfish is compared with Zhejiang shellfish, the former antitussive effect is obviously better than the latter.Therefore Anhui Becquerel makes the substitute of tendril-leaved fritillary bulb.(2) phlegm-dispelling functions experimental study shows, family's kind, tendril-leaved fritillary bulb that is wild, tissue cultures all have obvious phlegm-dispelling functions.Adopt the experiment of mouse PSP method, family plants and wild Bulbus Fritillariae Cirrhosae 200% liquid extract 25ml/kg, Bulbus Fritillariae Cirrhosae alkaloid 11.3mg/kg and bulb of fritillary saponin I ~ No. III 0.5mg/kg, all has more obvious expectorant effect, wherein stronger with the effect of alkaloid and No. III, saponin.To mouse peritoneal injection 3.9g/kg group training tendril-leaved fritillary bulb and wild bulb of fritillary aqua, have phlegm-dispelling functions too, after administration, 1 hour is effect peak value, and after 2 hours, effect declines, and by 1.5g/kg and 3.0g/kg administration, its expectorant effect strengthens with dosage and strengthens.Give rat Anhui shellfish alcohol extract 15g/kg (crude drug) gavage or general glycoside 26mg/kg oral, result shows, after administration, respiratory tract secretory volume significantly increases, and general glycoside least effective dose (LED) is 12mg/kg, maximal effective dose is 59mg/kg, strengthens with the increase of dosage in this scope effect of eliminating the phlegm.2. the effect of pair circulatory system, fritimine has hypotensive effect.Persistence blood pressure can be produced to cat intravenous injection fritimine 4.2mg/kg decline and accompany by of short duration respiration inhibition; Kashmirine also has hypotensive effect to anesthetized dog; Blood pressure can be made to increase when peimine and the peaceful minute quantity of peimine.Large number of biological alkali can hyperamization drops.3. antibacterial action, tendril-leaved fritillary bulb has certain antibacterial action.During 2mg/l concentration, the growth and breeding of Escherichia coli and staphylococcus aureus can be suppressed.Fritimine water logging agent (1: 25) has inhibitory action to star nocardia in vitro.4. toxic and side effect, (1) acute toxicity Wan Beijing alcohol extract 0.12mg/kg, to cat lumbar injection, observes breathing, blood pressure and electrocardiogram change all without exception.Give large and small mouse Anhui shellfish alcohol extract (or Anhui shellfish powder suspension, be equivalent to 120 times of clinical amount) 12g/kg gavage, observe 7 days, result large and small mouse whole show is as usual, and hair color, feed are all in normal range (NR).Mouse Anhui shellfish alcohol extract gavage minimum lethal dose is 40g/kg, is more than 300 times of clinical application amount.(2) long term toxicity test cavy 9, rabbit 4, by the continuous gavage of Anhui shellfish alcohol extract 6g/kg 20 days, routine pathology cut sections for microscopic examination are done to tracheae, the heart, liver, lung, kidney, brain, oesophagus, stomach and intestine, pancreas and adrenal gland etc., see a large amount of hypertrophy of goblet cell in tracheae and tunica mucosa bronchiorum, secretion in tube chamber is obviously thin, and other organs is showed no ANOMALOUS VARIATIONS.

 

Toxicity test:

Select SD rat, give the decoction of the Chinese medicine composition of the embodiment of the present invention 3 of variable concentrations (18.0,6.0,2.0g crude drug/kg), every day gavage once, continuous 90 days, within after last administration 24 hours, respectively group is alive kills 1/2 animal (male and female half and half), and all the other 1/2 animals continue observations 2 weeks rear living and kill.Duration of test observes outward appearance, general behavior, food ration, the changes of weight of animal, within after administration 90 days, within 2 weeks, carries out the index checking such as hematology (RBC, HB, granulophilocyte, PLT, CT, WBC and classification) and blood biochemical (AST, ALT, ALP, Glu, BUN, Crca, TP, T.BIL, ALB, CHOL), urine biochemistry, organ coefficient, Histopathology with drug withdrawal.Result of the test shows: Chinese medicine preparation of the present invention is good at high, medium and low dosage treated animal general state, and outward appearance sign, behavioral activity, food-intake and body weight increase change all without exception; Three dosage groups and control group hematological examination, blood biochemical analysis, urine biochemical analysis all in normal range (NR), without significant difference between group; Each group of main organs histopathological examination shows no obvious abnormalities.These parameters drug withdrawal also had no change after 2 weeks.This test dosage is respectively 180,60,20 times of clinical medicine dose, connect administration in 90 days according to result of the test Chinese medicine preparation of the present invention at high, medium and low three dosage (18.0,6.0,2.0g crude drug/kg) to have no significant effect rat, without clear and definite toxicity target organ and sensitive indicator, convalescence observation also has no retardance toxic reaction, points out the dose safety of Chinese medicine preparation clinical practice of the present invention higher.

 

Clinical trial

(1) material prepares

Buy 21 aa broiler chicken 400 from chicken plant of Jining of Shandong Province city, test for prevention and therapy.

Individual raiser provides 20 Day-old Broiler Chickens 1105 of natural infection infectious bronchitis of chicken, picks out the sick chicken 300 of manifest symptom, does therapeutic test.This chicken group, by gathering the typical cytopathic such as larynx, trachea-bronchial epithelial cell tissue from the chicken that dies of illness of the morbidity chicken group of doubtful infective bronchitis, uses conventional chicken embryo virus isolation procedure, is separated to the virus of doubtful infective bronchitis.The sick chicken of infective bronchitis is diagnosed as again by the serological tests such as AGP, ILT neutralization test and the test of viral physicochemical property and ILTV molecular biological testing.

(2) medicine

The Chinese medicine composition of embodiment 1-3 selected by Chinese medicine, and wherein pulvis is dissolved in water into the oral liquid of 0.2mg/ml; Hydrochloric acid sarafloxacin reaches logical medicine company by Zhejiang to be provided, and is dissolved in water into the oral liquid making 0.2mg/ml.

Chicken standard infectious laryngotracheitis kind poison is provided by Qingdao Hai Bo Bioisystech Co., Ltd, uses after multiple shape.

(3) rejuvenation of poison is planted

Get 20 SPF chicken embryos, laboratory hatching is to 10 ages in days.Infectious bronchitis of chicken standard strain sterile saline 1: 100 is diluted, through allantocherion vaccination, every embryo 0.2 mL, the chorioallantoic membrane of sterile collection pathology and allantoic fluid after 72 h, chorioallantoic membrane is placed in tissue grinder, add allantoic fluid gradually, fully grind, after-20 DEG C of multigelations 4 times, add 4000IU/mL penicillin and streptomycin, with centrifugation 15 min of 3 000 r/min, get supernatant, for subsequent use as kind of poison.

(4) experimental technique is prevented

400 21 Day-old Broiler Chickens are raised to 35 ages in days in animal housing, select healthy, uniform 200 of body weight, be divided at random prophylactic attack poison group 1, prophylactic attack poison group 2, prophylactic attack poison group 3, attack malicious control group and blank group four groups, often organize each 50, male and female ratio is impartial, prophylactic is attacked poison group 1-3 and is respectively and gavages the oral liquid of embodiment 1,2 and the decoction of embodiment 3, and attack malicious control group and gavage the hydrochloric acid sarafloxacin prepared, blank group gavages physiological saline.Often organize broiler chicken and gavage twice every day, gavage 5ml, for three days on end, prophylactic is attacked poison group 1-3 and is attacked malicious control group is diluted to 10 ~ 5 concentration respectively rejuvenation virus liquid with sterile saline at every turn, every chicken tracheal instillation 0.2 mL carries out artificial challenge, observes 6 d.

After attacking poison, chicken engenders rhinorrhea, sheds tears, and sneezes, and hear obviously hoarse cry night, drop-head closes order subsequently, lassitude, and the wing that hangs down squeezes heap, poor appetite, and drink, for increasing, has the phenomenon of indivedual chicken death.Through cuing open inspection to dead chicken, in nasal cavity, trachea-bronchial epithelial cell, there are faint yellow translucent serosity, mucus exudate as seen, air bag is muddy, and enlargement appears in bronchus, and bronchus and tracheae intersection have the symptoms such as hemorrhage, be diagnosed as morbidity, otherwise for prevent successfully.Record morbidity number, death toll, calculate the incidence of disease, the death rate and protective rate.

(5) Experiment on therapy method

The sick chicken of natural infection infectious laryngotracheitis of chicken 300, is divided into treatment group A, treatment group B and control group three groups, and treatment group A often only gavages the decoction of embodiment 3 by every day, every day 2 times; Treatment group B is by the feed of feeding embodiment 4 every day every; Control group gavages the hydrochloric acid sarafloxacin prepared, every day 2 times, and the treatment phase is 6 days, then observes 9 days, all cuts open inspection afterwards.

Clinical efficacy criterion: after treatment, sick chicken spirit, appetite recover normal, and symptoms such as shedding tears, have a running nose, sneeze disappears completely, cuts open the pathological change of inspection atypism, is recovery from illness, calculates cure rate.In therapeutic process, because infecting death, though during off-test dead cut open inspection still visible larynx and tracheae have pathological change that is hemorrhage, that ooze out, count and fail to respond to any medical treatment, account for the total ratio for the treatment of according to invalid number and calculate inefficiency.

(6) feeding and management of experimental animal

Broiler chicken adopts online flat foster, respectively organizes the conditions such as feed, drinking-water, illumination and feeding and management identical, and carry out conventional environment sterilization at whole duration of test.Natural sense catches an illness chicken for raising in cages, the same premorbid of treatments period feeding and management.

(7) result

A, prevention experimental result

Table 1 prevents experimental result

Group	Chicken sum (only)	Morbidity number (only)	The incidence of disease (%)	Death toll (only)	The death rate (%)	Protective rate (%)
							Poison group 1 is attacked in prevention	40	1	2.5	0	0	97.5
Poison group 2 is attacked in prevention	40	3	7.5	0	0	92.5
							Poison group 3 is attacked in prevention	40	2	5	0	0	95
Attack malicious control group	40	40	100	17	42.5	0
							Blank group	40	0	0	0	0	-

As can be seen from table 1, prophylactic attacks the morbidity sum of poison group 1-3 well below attacking malicious control group, without dead.The protective rate that prophylactic attacks poison group 1-3 is 97.5%, 92.5% and 95%.And the morbidity of attacking malicious control group adds up to 40, the incidence of disease is 100%, and death toll is 17, and sick chicken death rate is 42.5%.Blank group is without morbidity chicken.Chinese medicine composition of the present invention is described, have good prevention effect to the breathing problem of chicken especially infectiousness larynx bronchitis, protective rate is very high.

B, Experiment on therapy result

Natural infection infectious bronchitis chicken situation treated by table 2

Group	Chicken sum (only)	Death toll (only)	The death rate (%)	Inefficiency (%)	Recovery from illness number (only)	Cure rate (%)
							Treatment group 1	100	3	3	9	91	91
Treatment group 2	100	2	2	7	93	93
							Control group	100	18	18	41	59	59

As can be seen from table 2, natural infection chicken is with in traditional Chinese medicine composition for treating process of the present invention, and the symptom such as to have a running nose, shed tears alleviates gradually or disappears; Spirit, search for food, drink water and recover normal or normal gradually; Appetite obviously strengthens, and ight soil also transfers to normally.The 15th day after medication, all ill chickens were substantially all cured, and cure rate reaches 91%, and body weight also rises gradually.Proved by therapeutic test, Chinese medicine composition of the present invention has good curative effect to infectious bronchitis of chicken.

 

To sum up, adopt the Chinese medicine composition of embodiment of the present invention 1-3 and the feed of embodiment 4, can be good at prevention and therapy chicken respiratory tract disease especially infectious bronchitis of chicken, improve chicken autoimmunity, safe and effective, the object of the strong body that gets rid of illness can be reached.

 

The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.

Claims (8)

1. be used for the treatment of the feed of chicken respiratory tract disease, it is characterized in that, described feed comprises Chinese medicine composition and feed vector, and relative to the feed of 100 weight portions, the addition of described Chinese medicine composition is 10-20 weight portion;

In described Chinese medicine composition, the ratio of weight and number of each raw medicinal material is: root of large-flowered skullcap 5-15 part, Chinese ephedra 4-18 part, purple perilla 3-10 part, cordate houttuynia 5-16 part, golden cypress 3-9 part, capsule of weeping forsythia 4-12 part, dandelion 3-14 part, rhizome of cyrtomium 3-15 part, Radix Isatidis 5-20 part, folium isatidis 5-17 part, Radix Glycyrrhizae 5-15 part, lepidium seed 1-9 part, giant knotweed 1-10 part and Bulbus Fritillariae Cirrhosae 2-9 part;

Described feed vector is made up of the raw material of following ratio of weight and number: acorn nut powder 2-7 part, wheat bran 10-20 part, corn flour 10-30 part, beancake powder 10-20 part, Strong flour 5-15 part, sodium carbonate 1-3 part, sodium acid carbonate 1-3 part, fish meal 5-10 part and rice-chestnut powder 1-5 part.

2. the feed being used for the treatment of chicken respiratory tract disease according to claim 1, it is characterized in that, in described Chinese medicine composition, the ratio of weight and number of each raw medicinal material is: root of large-flowered skullcap 8-15 part, Chinese ephedra 4-12 part, purple perilla 5-10 part, cordate houttuynia 5-12 part, golden cypress 3-6 part, capsule of weeping forsythia 4-7 part, dandelion 8-14 part, rhizome of cyrtomium 9-15 part, Radix Isatidis 11-20 part, folium isatidis 9-17 part, Radix Glycyrrhizae 5-11 part, lepidium seed 1-5 part, giant knotweed 6-10 part and Bulbus Fritillariae Cirrhosae 5-9 part.

3. the feed being used for the treatment of chicken respiratory tract disease according to claim 1 and 2, it is characterized in that, in described Chinese medicine composition, the ratio of weight and number of each raw medicinal material is: root of large-flowered skullcap 5-11 part, Chinese ephedra 9-18 part, purple perilla 3-8 part, cordate houttuynia 8-16 part, golden cypress 5-9 part, capsule of weeping forsythia 6-12 part, dandelion 3-11 part, rhizome of cyrtomium 3-11 part, Radix Isatidis 5-14 part, folium isatidis 5-11 part, Radix Glycyrrhizae 8-15 part, lepidium seed 4-9 part, giant knotweed 1-7 part and Bulbus Fritillariae Cirrhosae 2-7 part.

4. the feed being used for the treatment of chicken respiratory tract disease according to claim 1-3 any one, it is characterized in that, in described Chinese medicine composition, the ratio of weight and number of each raw medicinal material is: root of large-flowered skullcap 7-13 part, Chinese ephedra 6-16 part, purple perilla 4-8 part, cordate houttuynia 7-13 part, golden cypress 3-9 part, capsule of weeping forsythia 4-12 part, dandelion 3-12 part, rhizome of cyrtomium 3-15 part, Radix Isatidis 5-20 part, folium isatidis 5-15 part, Radix Glycyrrhizae 5-15 part, lepidium seed 3-7 part, giant knotweed 3-6 part and Bulbus Fritillariae Cirrhosae 4-7 part.

5. the feed being used for the treatment of chicken respiratory tract disease according to claim 1-4 any one, it is characterized in that, in described Chinese medicine composition, the ratio of weight and number of each raw medicinal material is: the root of large-flowered skullcap 10 parts, 12 parts, Chinese ephedra, purple perilla 6 parts, cordate houttuynia 10 parts, golden cypress 6 parts, the capsule of weeping forsythia 8 parts, dandelion 7 parts, rhizome of cyrtomium 9 parts, Radix Isatidis 12 parts, folium isatidis 10 parts, 10 parts, Radix Glycyrrhizae, lepidium seed 5 parts, giant knotweed 5 parts and Bulbus Fritillariae Cirrhosae 5 parts.

6. the feed being used for the treatment of chicken respiratory tract disease according to claim 1-5 any one, is characterized in that, the formulation of described Chinese medicine composition is pulvis, and described preparation method comprises the following steps:

(1) take all raw medicinal materials according to ratio of weight and number, after pulverizing, drop into mixer and stir 40-60 minute, fully mix to obtain mixture;

(2) in mixture, add the water relative to mixture quality 2 ~ 4 times, decoct 2 ~ 4 hours, filter, in filter residue, add the water relative to 1 ~ 2 times, mixture, again decoct 1 ~ 2 hour, filter, by twice filtrate mixing, reduced pressure concentration removing moisture, obtain dry lotion, dry with spray dryer further and pulverize, sieve acquisition 50 ~ 100 order fine powder, obtains pulvis.

7. the feed being used for the treatment of chicken respiratory tract disease according to claim 1-6 any one, it is characterized in that, described feed vector is made up of the raw material of following ratio of weight and number: 4.5 parts, acorn nut powder, wheat bran 15 parts, corn flour 20 parts, beancake powder 15 parts, Strong flour 10 parts, 2 parts, sodium carbonate, sodium acid carbonate 2 parts, fish meal 7 parts and rice-chestnut powder 3 parts.

8. the feed being used for the treatment of chicken respiratory tract disease according to claim 1-7 any one, is characterized in that, the preparation method of described feed comprises the following steps:

(1) take all raw medicinal materials according to ratio of weight and number, after pulverizing, drop into mixer and stir 40-60 minute, fully mix to obtain mixture; The water relative to mixture quality 2 ~ 4 times is added in mixture, decoct 2 ~ 4 hours, filter, in filter residue, add the water relative to 1 ~ 2 times, mixture, again decoct 1 ~ 2 hour, filter, by twice filtrate mixing, reduced pressure concentration removing moisture, obtain dry lotion, dry with spray dryer further and pulverize, sieve acquisition 50 ~ 100 order fine powder, obtains pulvis;

(2) raw material is taken according to following ratio of weight and number: 4.5 parts, acorn nut powder, wheat bran 15 parts, corn flour 20 parts, beancake powder 15 parts, Strong flour 10 parts, 2 parts, sodium carbonate, sodium acid carbonate 2 parts, fish meal 7 parts and rice-chestnut powder 3 parts, add water Strong flour, sodium carbonate and sodium acid carbonate modulation, refining temperature is 55-60 DEG C, pour into after stirring in spraying machine and dry, pulverize acquisition fine powder;

(3) pulverize after the mixing of acorn nut powder, wheat bran, corn flour, beancake powder, fish meal and rice-chestnut powder, grinding particle size is 350-600mm, make fine powder amount reach more than 95% of total material, the fine powder obtained with step (2) mixes, and obtains feed vector;

(4) pulvis step (1) obtained and step (3) obtain feed vector mixing; put into multiplex mixer to stir 60 minutes ~ 110 minutes; ring mode bulking machine is put into expanded after taking-up; control the temperature of bulking machine between 80 DEG C ~ 95 DEG C; the expanded time is 1 ~ 2 hour; the aridity controlling material to be extruded is 90% ~ 95%; proceed to after taking-up in ring mode grit machine and be produced into precipitation hard particles feed; then sieve by 10 meshes, obtain described feed.