CN109197917A

CN109197917A - The Chinese medicine compound prescription conditioning liquid and its preparation method and application for preventing and treating hand-foot-and-mouth disease

Info

Publication number: CN109197917A
Application number: CN201710543208.1A
Authority: CN
Inventors: 郭卫宫; 严怡; 陈雪华; 王梓
Original assignee: Shanghai And Bio Polytron Technologies Inc
Current assignee: Shanghai And Bio Polytron Technologies Inc
Priority date: 2017-07-05
Filing date: 2017-07-05
Publication date: 2019-01-15

Abstract

The invention discloses a kind of Chinese medicine compound prescription conditioning liquids for preventing and treating hand-foot-and-mouth disease, it is mainly made of the bulk pharmaceutical chemicals of following parts by weight: 15-25 parts of radix scutellariae, 15-25 parts of coptis root, 15-25 parts of Cortex Phellodendri, 2-5 parts of Fructus Forsythiae, 8-12 parts of dried peppermint leaf, 2-6 parts of honeysuckle, 2-5 parts of Radix Glycyrrhizae, 2-6 parts of kuh-seng, 0.5-2 parts of tea polyphenols, 5-8 parts of cortex dictamni.In addition, invention additionally discloses the preparation method and application of the Chinese medicine compound prescription conditioning liquid.Experiment proves that Chinese medicine compound prescription conditioning liquid of the present invention can effectively inhibit spreading unchecked wantonly for Escherichia coli, staphylococcus aureus and Candida albicans, it is purified air, improve environment, the probability that hand-foot-and-mouth disease occurs is reduced, and effectively prevents hand-foot-and-mouth disease.

Description

The Chinese medicine compound prescription conditioning liquid and its preparation method and application for preventing and treating hand-foot-and-mouth disease

Technical field

The present invention relates to a kind of compound Chinese medicinal preparation more particularly to a kind of Chinese medicine compound prescription conditioning liquids for preventing and treating hand-foot-and-mouth disease. Moreover, it relates to the preparation method and application of the Chinese medicine compound prescription conditioning liquid.

Background technique

For skin as first of physiology defence line of human body and maximum organ, the moment participates in the functional activity of body, dimension The unity of opposites of body and natural environment is hold, any abnormal conditions of body can also be reflected in skin surface.

Hand-foot-and-mouth disease is shown on skin and oral cavity, but virus can invade the vitals such as the heart, brain, kidney.Children are endangered It is very big, it be easy to cause respiratory circulatory system failure.The enterovirus for causing hand-foot-and-mouth disease has more than 20 kinds, these enteroviruses are in liquid Very stable in body environment, virus can long-term surviving in external environment.

For the infection for preventing hand-foot-and-mouth disease, being first can be long-term in external environment by the enterovirus for causing hand-foot-and-mouth disease The phenomenon that survival, is controlled, and this field is badly in need of researching and developing the object of the present invention is to provide a kind of good antimicrobial effect, without hormone and west Medicine ingredient, safely, effectively, it is nontoxic, corrosion-free, without side-effects, to skin without any irritation, can be achieved to hand, face into The Chinese medicine compound prescription conditioning liquid of row disinfection, the infection sources of anti-hand-foot-and-mouth disease is controlled using the Chinese medicine compound prescription conditioning liquid.

Summary of the invention

One of the technical problem to be solved in the present invention is to provide a kind of Chinese medicine compound prescription conditioning liquid for preventing and treating hand-foot-and-mouth disease.

The second technical problem to be solved by the present invention is to provide the system of the Chinese medicine compound prescription conditioning liquid of the prevention and treatment hand-foot-and-mouth disease Preparation Method.

The second technical problem to be solved by the present invention is to provide answering for the Chinese medicine compound prescription conditioning liquid of the prevention and treatment hand-foot-and-mouth disease With.

In order to solve the above technical problems, the invention adopts the following technical scheme:

In one aspect of the invention, a kind of Chinese medicine compound prescription conditioning liquid for preventing and treating hand-foot-and-mouth disease is provided, it is mainly by following weight The bulk pharmaceutical chemicals composition of part: 15-25 parts of radix scutellariae, 15-25 parts of coptis root, 15-25 parts of Cortex Phellodendri, 2-5 parts of Fructus Forsythiae, 8-12 parts of dried peppermint leaf, 2-6 parts of honeysuckle, 2-5 parts of Radix Glycyrrhizae, 2-6 parts of kuh-seng, 0.5-2 parts of tea polyphenols, 5-8 parts of cortex dictamni.Preferably, 25 parts of radix scutellariae, it is yellow It is 25 parts of lotus, 25 parts of Cortex Phellodendri, 2 parts of Fructus Forsythiae, 9 parts of dried peppermint leaf, 4 parts of honeysuckle, 2.4 parts of Radix Glycyrrhizae, 2 parts of kuh-seng, 0.6 part of tea polyphenols, white 5 parts of fresh hide.

Raw medicinal material according to the present invention records Chinese medicine by Chinese Pharmacopoeia 2010 version one, and meets pharmacopoeial quality It is required that.

Radix scutellariae real name " a kind of reed mentioned in ancient books " is that have popular name " radix scutellariae " because careless color is yellow for a kind of reed mentioned in ancient books grass." a kind of reed mentioned in ancient books " word literal sense is " Folium Callicarpae Formosanae ".Not Name Radix Scutellariae, tsuchikane tea root.For Lamiaceae plant radix scutellariae (Scutellaria baicalensis Georgi), it is used as medicine with root. Nature and flavor and channel tropism: it is bitter, it trembles with fear.Return lung, gallbladder, spleen, large intestine, small intestinl channel.Major function: radix scutellariae has heat-clearing and damp-drying drug, cool blood miscarriage prevention, removing toxic substances Effect.Cure mainly warm heat disease, the infection of the upper respiratory tract, cough with lung heat, neonatal jaundice caused by dampness-heat, pneumonia, dysentery, hemoptysis, hot eyes, threatened abortion, The diseases such as hypertension, carbuncle swells furuncle sore.Originate in the ground such as Hebei, Liaoning, Shaanxi, Shandong, the Inner Mongol, Heilungkiang.Antibacterial action: radix scutellariae alcohol Immersion liquid 2g/ml concentration, Plating test have inhibiting effect to Escherichia coli, tangerine straw bacterium, staphylococcus aureus.Radix scutellariae is decocted Agent, alcohol extracting agent 1g/ml concentration, have inhibiting effect to meningococcus when being tested with flat band method.Antifungic action: radix scutellariae is decocted Liquid, test tube slant method 4% concentration of test inhibition dog denticle born of the same parents bacterium and trichophyton, 8% concentration inhibition oidium schoenleinii, 10% Concentration inhibits the mutation of oidium schoenleinii Mongolia, and 15% concentration inhibits homocentricity trichophyta and rust trichophyta.Radix scutellariae flooding agent 1:3 concentration is in vitro to trichophyton, Trichophyton concentricum, oidium schoenleinii, Microsporon audouini, wool Sample microsporum, epidermophyton rubrum, K, W, family name Epidermophyton, star nocardia etc. have different degrees of bacteriostasis.It is anti- Virus function: radix scutellariae decoction 25-100% concentration, in vitro test are replicated with inhibiting effect to hepatitis B virus DNA.It is anti-inflammatory anti- Allergy: 70% ethanol extract 200 of radix scutellariae, 500mg/kg stomach-filling baicalein, baicalin, wogonin 50,100mg/kg Stomach-filling can inhibit the exudation of mouse peritoneal caused by acetic acid and increase, to a kind of compound name of 48/80(, Sigma production) and cause Rat swollen enough also have inhibiting effect, 70% ethanol extract 500mg/kg stomach-filling of radix scutellariae, baicalein, baicalin and wogonin 100mg/kg stomach-filling has inhibiting effect to rat assist agent arthritis.Root of large-flowered skullcap water extract 100,200mg/kg stomach-filling, to rat Passive cutaneous anaphylaxis (PCA) has inhibiting effect, but to the contact of mouse caused by chloropicrin dermatitis (ear swelling) without obvious shadow It rings.It is baicalin and baicalein that radix scutellariae, which inhibits the effective component of passive cutaneous anaphylaxis (PCA),.Baicalin, baicalein are to reality Effectively, baicalin 10-4g/ml concentration can inhibit guinea pig trachea Schultz-Dale reaction 38%, and have antihistamine for the property tested asthma, Cholinolytic and the effect of papaverine sample.Baicalein is not soluble in water, two soluble derivative baicalein -6- disodium hydrogen phosphate (BPS) And baicalein -6- disodium sulfate (BSS) 5mg/kg intravenous injection before antigen is attacked, there is inhibiting effect to P of Rats CA；10mg/ Kg intravenous injection is attacked first 10 minutes in antigen and is administered, and has inhibiting effect to cavy allergic asthma；BPS and BSS are in 10- 4g/ml concentration has inhibiting effect to isolated guinea pig intestinal tube and tracheae chultz-Dale reaction；BPS and BSS 5mg/kg vein Injection, has inhibiting effect to rat Reversed cutaneous anaphylaxis (RCA)；5-10mg/kg intravenous injection also inhibits Forssman skin Skin vasculitis, but Arthus reaction is had no significant effect.On baicalein and BPS structure with antiallergic disodium chromoglycate (DSCG) It is similar, but DSCG is double-colored ketone, and baicalein and BPS are monochromatic ketone, and DSCG is antibody-mediated to reagin (Reagin) Allergic reaction has high degree of specificity, and to the antibody-mediated allergic reaction of non-reagin (IgG) there are few effect, PBS then not only presses down The allergic reaction that reaginic antibody processed mediates, if PBS 10-6-10-4g/ml inhibits monkey lung medium caused by antigen to discharge, PBS 200mg/kg intraperitoneal injection inhibits allotransplantation in rats PCA, and the rat hypertrophy cell that 10-4g/ml inhibits reaginic antibody to mediate de- Grain etc., the reaction for also inhibiting non-reaginic antibody to mediate are situated between as BPS10-4g/ml can inhibit the release of egg albumen sensitization Guinea pig lung People's lung caused by matter and inhibition anti-IgE antibodies discharges Anaphylactic mediator, it is believed that and two chromones are internuclear in DSCG molecule certain distance, It can be combined in reaginic antibody functional location, and be not suitable for non-reaginic antibody.Other effects: baicalein 10,20mg/kg Intravenous injection, has diuresis to anesthetized dog.Radix scutellariae aqua 4g/kg stomach-filling has prevention and treatment to make rat galactose-cataract With the formation of cataract can be delayed.Baicalin has an inhibiting effect to Rat Lens aldose reductase, and ID50 is 1.81 × 10(-3)mg/ml.Baicalin 150mg/kg stomach-filling, to blood glucose in diabetic rats level caused by broneomycin without being decreased obviously, But Red Blood Cell Sorbitol Content significantly reduces after treatment, prompts also to play the role of inhibiting aldose enzyme in animal body, it is possible to Prevention and treatment for diabetic complication.Baicalin, baicalein and wogonin 50-125 μ g/ml are to Mouse Liver sialidase There is inhibiting effect.Baicalin 100mg/kg, glucuronic acid 43mg/kg subcutaneous injection can fight mouse caused by strychnine Death, and glucoside member baicalein is invalid, it is believed that the detoxication that the glucuronic acid after baicalin hydrolysis rises.Radix scutellariae is metabolized PGs There is wide influence, water extract has inhibiting effect to the biosynthesis of PGs.Radix scutellariae-chemical composition of Chinese materia medica radix scutellariae scutellariae,radix contains Flavone compound: baicalein, neobaicalein, baicalin (baicalin), wogonin, wogonoside, oroxylin A, 7- Methoxyl group baicalein, skullcapflavone, dihydro oroxylin, the effective components such as Chrysin.Pharmacology: radix scutellariae toxicity is extremely low, decoction Rabbit stomach-filling is given, alcohol extract intravenous injection only weakens in activity, and 4 g/kg of radix scutellariae preserved material also had no any poison to dog stomach-filling 8 weeks Property reaction, such as yellow mustard preserved material 2g/kg is injected intravenously in Healthy Rabbits, is first behaved with composure, it is later dead, it is seen that be injected intravenously and compare mouth Taking poison property is much bigger.Baical Skullcap root P.E muscle and intravenous injection can make to significantly reduce in the normal rabbits total white blood cells short time. It is 3081mg/kg that baicalein, which is injected intraperitoneally to the median lethal dose of mouse,.Radix scutellariae preserved material 15g/kg/ stomach-filling has vomiting existing dog As 5g/kg stomach-filling continuous 8 weeks, keeping dog loose and watery stool soft.

Coptis root alias, Zhi Lian.For cohosh coptis root (Coptis chinensis Franch.), Coptis Beltoidea C.Y.CHENG et HSIAO (Coptis deltoidea C. Y. Cheng et Hsiao) and cloud connect the dry rhizome of (Coptis teeta Wall.). It practises respectively and claims " taste company ", " refined company ", " cloud company ".Meridian distribution of property and flavor: (root) is bitter, trembles with fear, nontoxic.Return heart, spleen, stomach, liver, gallbladder, large intestine channel. Major function: heat-clearing and damp-drying drug, purging fire for removing toxin.For damp and hot feeling of fullness, acid regurgitation is vomitted, dysentery, jaundice, unconsciousness due to high fever, heart-fire hyperactivity, Dysphoria and insomnia, blood-head tells nosebleed, hot eyes, toothache are quenched one's thirst, carbuncle swells furunculosis；Eczema, wet sore, purulent ear canal are controlled outside.Wine coptis root is apt on clear It is burnt burning hot.For hot eyes, aphtha.Turmeric lotus clearing stomach anti-vomiting.It is mutually tied for fever and chills, damp and hot middle resistance, feeling of fullness vomiting.Cornel coptis root Easypro liver anti-vomiting.For liver-stomach disharmony, acid regurgitation is vomitted.Antibacterial action: damp and hot retention of damp-heat in the interior, stomach, vomiting, dysentery etc. are used for Disease.With radix scutellariae, rheum officinale etc., the card of retention of damp-heat in the interior can be controlled.It is reluctant to leave stomach to damp and hot, often cooperates the tuber of pinellia, caulis bambusae in taenian；With radix aucklandiae, radix scutellariae, Pueraria lobata etc. is to control dysentery.In recent years coptis root application must be replaced by clinically commonly using coptis root, and the coptis root cellulose content in coptis root palpus is 1.2% left Right extracorporeal bacteria inhibitor test shows: 50% coptis root palpus decoction is identical as the antibacterial efficacy of 10% coptis root decoction.Resisting pathogenic microbes: Antibacterial action staphylococcus aureus, hemolytic streptococcus, pneumococcus, diplococcus meningitidis, shigella dysenteriae, bacillus anthracis Deng.Antivirus action, inhibition influenza virus, hepatitis B etc..Antiprotozoan effect, in vitro inhibit Amoeba, Trichomonas vaginalis, Trypanosome.Detoxication: confrontation bacteriotoxin reduces staphylococcus aureus coagulase, hemolysin potency, reduces Escherichia coli Virulence.Inhibit kinds of experiments inflammation, effective component is jamaicin, and Anti-inflammatory Mechanism is related with stimulation corticotropin release.Solution Heat effect is related with the generation of the area maincenter PO/AH neuron cAMP is inhibited.Pharmacological action: coptis root studies have shown that absorb, distribution, Excretion jamaicin is oral to be not easy to absorb the parenteral administration of, tissue is quickly entered after absorbed into serum, blood concentration is not easy to maintain；The mankind It is about 20mg% that 30 minutes blood concentrations, which are 100 μ g %(extracorporeal disinfecting concentration, after oral 0.4g Berberine hydrochloride), then gradually It reduces, even if repeat administration, every 4 hours 0.4g, blood concentration, which also loses, increases in vivo, and nearly all tissue has barberry The distribution of alkali, and it is very of short duration with the time that it is stored in each tissue for most such as the heart, kidney, lung, liver, after 24 hours only It is micro, it is mainly metabolized in vivo, also has small part (6.4%) after kidney discharge rabbit is oral, can also absorb, and can be It is stopped 72 hours in blood, also there is excretion in urine, with concentration highest in heart in tissue, pancreas, liver take second place Oral Administration in Rats, inhale Little drug administration by injection is received, then primarily enters the heart, pancreas, liver, omentum majus fat；Only have pancreas after 24 hours, can still look into and see in fat Considerable amount of jamaicin only a small amount of (1%) is discharged from urine.

The main component of obakunone Cortex Phellodendri bark is jamaicin (Berberine).Meridian distribution of property and flavor: it is bitter, it trembles with fear.Enter kidney, bladder Through.Major function: heat-clearing.Eliminating dampness, purging intense heat, removing toxic substances.Hot dysentery is controlled, diarrhea is quenched one's thirst, jaundice , crippling wilt, nocturnal emission, stranguria with turbid discharge, hemorrhoid, just Blood, also under leukorrhea, hectic fever due to yin labor heat, red eye, swell pain, aphthae, sore swollen toxin.Pharmacological action: Cortex Phellodendri is same as coptis root contain it is more Jamaicin, therefore its pharmacological action is also substantially similar, referring to coptis root item.But content is low compared with coptis root, and contains other compositions, effect Also some differences.Antibacterial action: Cortex Phellodendri antibiotic effective ingredient is jamaicin, therefore its pharmacological action is substantially similar to coptis root, but contains It measures low compared with coptis root.In vitro test is to staphylococcus aureus, pneumococcus, corynebacterium diphtheriae, Streptococcus viridans, shigella dysenteriae (except sonnei), hemolytic streptococcus, meningococcus, comma bacillus, bacillus anthracis effectively or have stronger inhibition to make With；Also there is inhibiting effect to hay bacillus, Bordetella pertussis, clostridium tetani；To H37Rv, mycobacterium tuberculosis without directly suppression Production is used, but bacterium number can be made to reduce, but bacteriostasis is presented in when concentration is up to 1%.Mycobacterium hominis (H37Rv) is being inoculated with cavy When making experimental therapy, takes orally or the curative effect of injection is very poor.To the cavy of inoculation Mycobacterium bovis, the hydrochloric acid knot of phellodendron extract When brilliant object intramuscular injection, there is certain curative effect.Cortex Phellodendri take a favorable turn to the clinical symptoms of tuberculosis patient and x-ray inspection, and is better than coptis root. In test tube, Cortex Phellodendri decoction or preserved material have different degrees of bacteriostasis to common pathogenic fungus.Its water decoction cannot kill Dead Leptospira (dosage need to be I times big compared with coptis root).Also play the role of to Trichomonas vaginalis in vitro weak.It is reported that Cortex Phellodendri soda acid 1:1 Cortex Phellodendri liquid is made in method extract, can treat bacillary dysentery with enema, can fully recover within general 2-4 days.Cortex Phellodendri treatment solution DC conductance Enter and treat chronic prostatitis, total effective rate is up to 97.3%.Antifungic action: the ether extract of CORTEX PHELLODENDRI AMURENE and CORTEX PHELLODENDRI CHINENSE is to new Type cryptococcus and trichophyton purpureatum have stronger bacteriostasis, and effect is stronger than nystatin, but the suppression to Candida albicans It makes weak of being enjoyed than mildew making.Antitussive effect: the volatilization of Cortex Phellodendri fruit has antitussive effect, and antibechic ingredient is mainly spiceleaf Alkene.And get jamaicin and 5 from volatile oil, 5'- dimethyl furfural ether.Other effects: phellodendrine and former times sieve woods are to maincenter Nervous system has inhibiting effect, and the spontaneous activity of mouse, various reflections are suppressed；Non- Anaesthetic Rabbits former times sieve woods is given, Brain wave may occur in which amplitude slow wave.Phellodendrine has slight curariform action mask, on frog rectus aabdominis tensity without influence, but can inhibit The contractile response as caused by acetylcholine.Pharmacological action: to isolated rabbit intestines, Cortex Phellodendri powder can enhance its amplitude, and obakunone can enhance Its tension and amplitude；Obakulactone then inhibits intestinal tube.With the rabbit with pancreas fistula, Cortex Phellodendri has the work for promoting pancreatic secretion With.Obakulactone can reduce rabbit blood glucose, obakunone is without this effect when close to lethal dose (0.05-0.1g/kg).It is said that Cortex Phellodendri Ketone plays the role of protecting blood platelet.Medicine (poison) is of science: the intraperitoneal injection of Cortex Phellodendri liquid extract, and the median lethal dose to mouse is 2.7g/ kg.It is 69.5mg/kg that phellodendrine, which is injected intraperitoneally to the median lethal dose of mouse,.Cortex Phellodendri fruit volatile oil stomach-filling is to mouse Median lethal dose is 6.683 ± 0.656g/kg.

Fructus Forsythiae (Classification system: Forsythia suspensa), meridian distribution of property and flavor: bitter, it is slightly cold.Return lung, the heart, small intestine Through.Major function: clearing heat and detoxicating, dispersing swelling and dissipating binds.For ulcer, scrofula, acute mastitis, erysipelas, anemopyretic cold, warm disease is from the beginning of warm It joins the army, hyperpyrexia polydipsia, coma macular eruption, heat gonorrhea renal shutdown.Pharmacological action: antibacterial, antivirus action: 1.1. antibacterial actions: will even It sticks up Volatile Oil from Seed emulsion and measures dilution method progress antibacterial tests again with test tube, the medicine removes slightly lower to Pseudomonas aeruginosa effect as the result is shown Outside, there is stronger antibacterial action to other 10 plants of Gram-positives and negative bacteria.Wherein to the work of staphylococcus aureus With most strong (antibacterial potency 1/1.024)；It secondly is pneumococcus；First, beta streptococcus；Catarrh Nai Shi coccus；Fu Shi dysentery Bacillus；Paratyphoid bacillus A；(antibacterial potency is 1/512) and Escherichia coli (antibacterial potency 1/256), to pneumonia bar Bacterium, proteus vulgaris antibacterial potency are 1/28.The experimental liquid for having antibacterial action to tried bacterium is chosen, secondary culture again is carried out Test, Volatile Oil of Forsythia Suspensa Seeds more thoroughly and stablizes the antibacterial action of tried bacterium as the result is shown.Weeping forsythia concentrated decoction There is antibacterial action in vitro, can inhibit typhoid bacillus, paratyphosum Bacterium, Escherichia coli, shigella dysenteriae, corynebacterium diphtheriae and cholera arc Bacterium, staphylococcus, streptococcus etc..The bacteriostasis of Fructus Forsythiae in vitro is substantially similar to honeysuckle；For the master of antibacterial in Yinqiao San Want ingredient.Honeysuckle is seemingly more than Fructus Forsythiae to the inhibiting effect of Salmonella, especially typhoid bacillus and hemolytic streptococcus, And to the inhibition of shigella dysenteriae, staphylococcus aureus then with Fructus Forsythiae like preferable.The two is used in combination, and has no collaboration in test tube Effect, the compound formed with coptis root, radix scutellariae, In Vitro Bacteriostasis ratio are stronger according to cloud when being applied alone Fructus Forsythiae.Antibacterial is effective in Fructus Forsythiae Composition Study is few, and forsythol is 1:5120 to the Mlc of staphylococcus aureus in trial operation；It is 1 to shigella dysenteriae: 1280；It is 1:640, possible power antibiotic effective ingredient to corynebacterium diphtheriae and paratyphoid (first) bacillus.It spends to mouse experiment tuberculosis Disease has certain curative effect, then invalid to cavy.In addition, also reporting the effect of its anti-mycobacterium tuberculosis in test tube and clinically. The alcohol extracting thing of Fructus Forsythiae has anti-leptospira effect in vitro, intensity not as good as coptis root, Common Sage Herb or honeysuckle, radix scutellariae, and It is similar to Cortex Phellodendri, paris polyphylla.Fructus Forsythiae flooding agent (1:5) is in vitro to some inhibiting effect of star nocardia.1.2. antimycotic Effect: Volatile Oil of Forsythia Suspensa Seeds emulsion is measured into dilution method with test tube again and carries out anti-white and Candida tropicalis test, is as a result shown Show that the medicine has obvious antibacterial action to two bacterial strains tried, antibacterial potency is respectively 1/1.024 and 1/512. 1.3. antivirus action: Volatile Oil of Forsythia Suspensa Seeds emulsion is resisted with method in chicken embryo (drug concentration 1/32) and the outer method of chicken embryo Asia influenza A virus and I type parainfluenza virus Experiment on Function.It can be seen that Volatile Oil of Forsythia Suspensa Seeds exists to capital section 68-1 pearl virus The energy display effect that is administered simultaneously is infected, the administration energy display before infection is administered simultaneously and infects of celestial platform strain virus acts on.It can see Volatile Oil of Forsythia Suspensa Seeds emulsion has significant antivirus action to 68-1 plants of capital section and celestial platform strain virus outside chicken embryo out.To institute Trying two kinds of viral antiviral activities is 1/65,536.With spot hybridization test, Fructus Forsythiae decoction 25%-100% concentration, there is drop The effect of low HBV DNA (HBVDNA) content.Influence to cardiovascular system: 2.1. Fructus Forsythiae injection In the influence of the body cat heart when liquid is to toxic shock: experiment uses cat 3, and intraperitoneal anesthesia, arteria carotis intubation measures blood pressure, and tracheae is inserted Pipe, even phrenoton, opens thoracic cavity and pericardium, and the apex of the heart is coupled to micro- micro-pull sensing instrument with silk thread, with three by exposure heart Recorder traces heart and relaxes contracting curve.Before experiment, first traces one section of cardiac muscle and relax to contract and involve blood pressure curve as normal control, so Be injected intravenously afterwards typhoid vaccine 0.8-2.5ml/kg more apparent damage to appear myocardium curve (the easypro contracting wave height of cardiac muscle is low uneven, The rhythm and pace of moving things is not whole), blood pressure decline.After being injected intravenously Fructus Forsythiae injection 8g/kg, the rhythm of the heart becomes the neat wherein easypro contracting wave-amplitude of 2 cat cardiac muscles Increase, blood pressure rises about 40mmHg after 30 minutes.Prompt has heart tonifying and boosting.2.2. to the shadow of capillary permeability Sound selects healthy mice male and female to be regardless of, and weight 18-22g is divided into experimental group and control group, 7 animals of experimental group, by tail vein or It is subcutaneously injected Fructus Forsythiae injection (60g/kg), 9 animals of control group, with physiological saline alternatives to medication (0.4ml/ is only).10 minutes Only by 0.5% Azo-Blue liquid 0.2ml/ of tail vein injection.After 30 minutes, 0.4% acetic acid 0.4ml/ of intraperitoneal injection is only.30 points again Mouse collapse due to massive hemorrhage is put to death after clock, abdominal cavity normal saline flushing peritoneal exudate is opened, eluate is placed in 5ml scale test tube extremely Scale is centrifuged 10 minutes with 2000 revs/min, supernatant is taken to measure its Azo-Blue concentration.As a result control group peritoneal exudate she Wen's indigo plant concentration (μ g%) is 368.47 ± 28.55 (mean+SDs, similarly hereinafter), and experimental group is 160.12 ± 28.37 (P < 0.01).Show that capillary permeability caused by Fructus Forsythiae injection Dichlorodiphenyl Acetate has obvious inhibiting effect.Suppression to rat small intestine in vitro Production is used: cavy is used in experiment, makees in vitro intestines sample by Magnur method.Rat small intestine in vitro is placed in 12 × l0-2 Fructus Forsythiae nutrient solution, altogether 10 tests are done, as a result show as the reduction of intestines tension for 4 times, are fallen after rising for 6 times.Contractive amplitude 8 times decreases, 2 times unchanged.Deng appearance Physiological saline is measured in vitro intestines without influence.Prompt Fructus Forsythiae has inhibiting effect to guinea pig in vitro small intestine.Inhibit elastin laminin enzyme activity Effect: Fructus Forsythiae etc. is diluted to every 1ml containing 1.5g, 0.75g and 0.375g, 3 kinds of concentration.Porcine pancreatic elastase (PPE) is diluted to 19u/0.5mgml and two kinds of concentration of 9.5u/0.25mgml.By the enzyme solution of 19u/0.5mgml and above-mentioned various concentration After drug distinguishes equivalent mixing, 10 μ l are respectively taken, are respectively dropped into agar plate hole, stay two holes respectively to drip 10 μ l, 9.5u/ in each ware The enzyme solution of 0.25mg.ml makees enzyme dissolution parallel control, 37 DEG C of incubations measurement dissolution ring diameter after 24 hours.(enzyme activity and dissolution Ring diameter is linear).Fructus Forsythiae inhibits the effect of elastin laminin enzyme activity to be shown in Table 5.It can be seen that the Fructus Forsythiae of three kinds of concentration can be shown Inhibit 0.095u PPE enzyme activity (P < 0.001), wherein in 7.5mg concentration completely inhibit PPE enzyme activity.Fructus Forsythiae water Dissolubility crude extract 10-3g/ml, 10-4g/ml have inhibiting effect to phosphodiesterase.Antiradiation injury: Fructus Forsythiae methanolic extract 1g/kg intraperitoneal injection, is administered for 5 minutes before roentgen radiation x, has protective action to mouse skin damage.

Dried peppermint leaf 1%-1.46% containing stain main is divided into levorotatory menthol (menthol), content 62.3%- at peppermint in oil 87.2%, also contain left-handed menthones (menthone) isomenthone (isomenthone), pulegone (pulegone), the acetic acid last of the ten Heavenly stems Ester (decyl acetate), menthyl acetate (menthyl acetate) methyl benzoate (methylbenzoate), α-and Nopinene (pinene), β-thujene (β-thu-jene), 3- amylalcohol (3-pentol), 2- alcohol (2-hexanol), 3- octanol (3-octanol), dextrorotation laurene (myrcene), limonene (limonene) and Cineole (cineole), alpha-terpineol (α- Terpineol) etc..Contain flavones ingredient again: different Rui Fuling (iso-raifolin (, luteolin -7- glycoside (luteolin-7-glucoside), peppermint daidzin (methoside);Determination of Organic Acids: Rosmarinic acid (lusmarinic Acid), caffeic acid (caffeic acid)；Chloro sour component: aspartic acid (aspartic acid), glutamic acid (glutamic Acid), serine (alanine), asparagine (asparagine), valine (valine), leucine (leuine) and different Leucine (isoleucine), phenylalanine (phenylalanine), methionine (methionine) lysine (lysine). Dried peppermint leafThe pharmacological action of extracting solution is as follows: antivirus action:Peppermint fresh leafWater decoction 1:20 concentration, to ECHO11 plants viral There is inhibiting effect.Analgesia, itching-relieving action:Peppermint fresh leafMainly make external use anti pruritic, faint local anaesthesia and counteirritantia, is applied to office Portion causes cool feeling due to stimulation nerve, and inhibits pain nerve.Anti-irritant, antitussive action:Peppermint fresh leafAnti-irritant effect Cause tracheae to generate new secretion, and the mucus of stiff is made to be easy to be discharged, therefore there are phlegm-dispelling functions, also has been reported that menthol brain to globefish Mouse and there is good antitussive action per capita.Bactericidal effect:Peppermint fresh leafThere is very strong bactericidal effect.

Honeysuckle, chlorogenic acid (chlorogenic acid).Meridian distribution of property and flavor: sweet, slight bitter, faint scent is pungent, trembles with fear.Return lung, stomach, The heart, large intestine channel.Major function: hemostasis, analgesic.Nosebleed epistaxis is controlled, is had a stomachache, is vomited and diarrhoea.Resisting pathogenic microbes effect: diplococcus meningitidis, Pseudomonas aeruginosa, tubercle bacillus are also effective.Flooding agent is stronger than decoction effect, and leaf decoction is stronger than the effect of colored decoction.If being shared with Fructus Forsythiae, Antibacterial range can also be complementary；It is shared with penicillin, penicillin can be reinforced to the antibacterial action of resistant Staphylococcus aureus, this can Can play the role of collaboration on inhibiting the synthesis of bacterium vivo protein.Anti-inflammatory and refrigeration function: intraperitoneal injection honeysuckle is extracted Liquid 0.25g/kg can inhibit Rat Carrageenan colloidality foot swollen.30~40g/kg of honeysuckle flower injection can mitigate egg white according to another report Property foot swell degree.Flos Lonicerae extractive solution 8g/kg is injected intraperitoneally, 2 times/day, continuous 6 days, to rat crotons oiliness granulation capsule, also has It is obvious it is impervious go out and anti-proliferate effect.Reporting honeysuckle has apparent refrigeration function, but with cholera vaccine, potato Bacillus, hay infusion etc. are injected to family's rabbit ear vein, and pyrogenicity does not confirm that honeysuckle decoction 5g/kg stomach-filling has antipyretic effect, it is believed that This may be different with the tolerance of the honeysuckle preparation, dosage or the rabbit that use related.The effect of booster immunization function: honeysuckle is decocted Dilution agent remains to the phagocytic function for promoting leucocyte to the concentration of 1:1280.Mouse peritoneal injects honeysuckle flower injection, also has bright The aobvious effect for promoting inflammatory cell phagocytic function.Central excitation effect: it is proved through the kinds of experiments method such as electroconvulsive shock, rotating cage oral After chlorogenic acid, the animal central nervous systems such as rat, mouse can be caused excited, action intensity is the 1/6 of caffeine, and the two is closed With without addition and humidification.Effect for reducing blood fat: rat oral gavage honeysuckle 2.5g/kg can be reduced enteral cholesterol absorption, reduce Cholesterol level in blood plasma.Experiment in vitro is it has also been found that honeysuckle can combine with cholesterol, but Simiao Yongan Tang is (honeysuckle, profound Ginseng, Radix Angelicae Sinensis, Radix Glycyrrhizae) treatment Experimental Atherosclerosis in Rabbit.Reducing blood lipid and Cholesterol concentration in aortic tissue content have not been observed Effect.Antiendotoxin: endotoxin content is measured with limulus test, 300% honeysuckle (honeysuckle) injection is dilute with 1:2~1:64 It releases, no matter in vitro test uses female tab method or test tube method, and the endotoxin content being substantially reduced in test solution, wherein 1:2~1:8's is dilute It releases pipe to be in a liquid state as negative control pipe, positive control is in gel.Honeysuckle (honeysuckle) distillate 6g/kg intravenous injection, Rabbit temperature decline caused by 2.8mg/kg mutual to Pseudomonas aeruginosa endogenous toxic material is injected intravenously and leukocyte count decline have antagonism, gold and silver Flower (honeysuckle) distillate 7.5g/kg or injection 2.5g/kg intraperitoneal injection, is injected intraperitoneally Pseudomonas aeruginosa endotoxin 65mg/kg Mouse have a protective effect, reduce mouse death rate.Other effects: once there are the water and steeping in wine of in-vitro screening laboratory report honeysuckle Liquid has apparent cytotoxicity to sarcoma 180 and ehrlich ascites cancer.Honegsukle flower P.E is oral to Experimental Gastric Ulcer in Rats There is slight preventive effect.N of high dose oral chlorogenic acid can increase gastrointestinal peristalsis, promote gastric juice and bile secretion.Chlorogenic acid and its point Solution product has excitation to Isolated Rat Uterus.In addition, chlorogenic acid can also slightly enhance adrenaline and its noradrenaline Element is to the boosting of cat and rat, but on the nictitating membrane response of cat without influence.

Radix Glycyrrhizae, (scientific name: Glycyrrhiza uralensis Fisch), meridian distribution of property and flavor: sweet and neutral, it is nontoxic.Bandit ancestor says: Raw then micro- cool, taste is bad；Toast is then warm.Enter spleen, stomach, lung channel.Major function: vital organs of the human body fever and chills perverse trend, hard muscles and bones, long muscle, Times strength, metal-inflicted wound, removing toxic substances.Pharmacological action: Western modern medicine theories discovered that Radix Glycyrrhizae agent has an anti-inflammatory and resistance state reflection function, therefore Doctor trained in Western medicine is clinically mainly as moderator.Alleviate cough, pharyngalgia laryngitis is treated in eliminating the phlegm;Radix Glycyrrhizae or enoxolone have deoxidation cortex Ketone effect, has good effects to chronic adrenocortical hypofunction;Glycyrrhiza preparation can promote gastric mucus to be formed and divided It secretes, extends epithelial cell life, have anti-inflammatory activity, be usually used in the treatment of chronic ulcer and duodenal ulcer;The flavones of Radix Glycyrrhizae With anti-inflammatory, spasmolysis and antiacid effect;Radix Glycyrrhizae is also one of primary raw material of jintan.Effect to digestive system: Radix Glycyrrhizae is removed Flavonoid glycosides have obvious protective function to Rat Experimental ulcer in the medicinal extract of glucin and Radix Glycyrrhizae.Cortex hormone of aadrenaline sample is made With: extract of licorice root, glycyrrhizin and enoxolone have promotion sodium, the effect of water retention to Healthy People and animal;It is low dose of sweet Careless glucin (every 100ug) can be such that rat chest gland atrophy and adrenal gland weight increases, and generate glucocorticoid cortisone sample and make With.Then the effect of glucocorticoid sample is unobvious when large dosage of, and the effect of mineralocorticoid sample is only presented.Detoxication: extract of licorice root And glycyrrhizin is poisoned to some drugs, the poisoning of food poisoning, interior metabolism product has certain detoxification ability.Removing toxic substances is made Effective ingredient is glycyrrhizin, and detoxication mechanisms are that glycyrrhizin has suction-operated, glycyrrhizin hydrolysate Portugal to poisonous substance Grape uronic acid can be in conjunction with poisonous substance and glycyrrhizin is known as adrenocortical hormones fuction, enhances the detoxification ability etc. of liver The result of aspect combined factors effect.Function of relieving cough and calming asthma: enoxolone has apparent central antitussive effect, and large dosage of is sweet Careless hypo acid can be such that mouse breathing inhibits.Furthermore glycyrrhizin, glycyrrhetate still have anti-inflammatory and antiallergy, anti-liver injury, resist Promote cancer, antibacterial, anti AIDS virus (glycyrrhizin) effect.Adjust body's immunity, anti-arrhythmia.Medicine (poison) is of science: sweet The pharmacological action of grass has the effect of cortex hormone of aadrenaline (hormone) sample, much knows excessive Radix Glycyrrhizae to what Radix Glycyrrhizae was recognized people The discharge of urine volume and sodium (sodium, salt contain composition) can be made to reduce, body can accumulate excessive sodium (salinity) and cause high blood Pressure；Moisture storage capacity increases, and will lead to oedema.Hypokalemia caused by excessive blood potassium is lost simultaneously, leads to arrhythmia cordis, muscle It is powerless.Electron microscope observation shows that Radix Glycyrrhizae can cause myocardial damage.These results of study, the medical literature in west is printed already Out.

Kuh-seng (matrine), meridian distribution of property and flavor: it is kuh-seng bitter, cold in nature, return liver, kidney, stomach, large intestine channel.Major function: kind drop It lets out；With heat-clearing and damp-drying drug benefit water removing jaundice, the effect of wind dispelling insecticide.Heat is gone mad when curing mainly, and heat toxin accumulation of pathogens in chest is quenched one's thirst, damp-heat dysentery, intestines Wind hematochezia, leukorrhagia pruritus vulvae, jaundice, hot urination, rubella itch, scabies, leprosy, wet poison sore.Antibacterial action: 1. kuh-seng ethers mention Object and alcohol extract have stronger bacteriostasis to staphylococcus aureus；Kuh-seng flooding agent is to trichophyton, proper alignment hair tinea Bacterium, Trichophyton schoenleini, Du small gemma tinea bacterium of big belly difficult to understand etc. have inhibiting effect.2. anti-inflammatory effect: matrine causes mouse knoting oil Auricle edema, the exudation of mouse peritoneal caused by acetic acid increase, Rat Carrageenan colloidality footpad swelling, have inhibiting effect.It is flat Asthma phlegm-dispelling functions: matrine mainly passes through excited beta receptor, and the beta receptor of especially excited maincenter releases bronchial spasm and suppression The release of antibody processed and slow reacting substance and generate antiasthmatic effect.Matrine is having calcium or without in calcium kirschner nutrient solution, equal energy Effect to anti-acetylcholine barium chloride excitement isolated guinea pig, rat trachea and intestinal tube.Banlangen, oxymatrine are to histamine Cause the antiasthmatic effect of asthma cavy substantially similar to aminophylline, rate of relievining asthma for 1 hour is all 90% or more, and control group is 15% or so； It relievings asthma on the time, aminophylline is 55% in 6 hours rates of relievining asthma, and Banlangen is then 80% or more.The cause asthma of sophocarpine Ach acts on ratio Aminophylline is strong, and antiasthmatic effect may be by the beta-receptor of excited midbrain and work.Phenol red excretion method proves, intragastric administration on mice Banlangen, flavones 0.8g/kg have what is said or talked about effect of obviously dispelling.Stable effect: Banlangen 50-100mg/kg can obviously inhibit small Mouse move freely；400mg/kg obviously inhibits mouse passive activity；100-200mg/kg can inhibit the Ou Dou of lonely mouse Attack；Banlangen 25-40mg/kg is shared with wintermin (5mg/kg), can cause mouse righting reflex loss, 100- 200mg/kg Banlangen can obviously reinforce 0.5mg/kg reserpine to the inhibiting effect of spontaneous activity in mice；Banlangen 50- The yellow Jackets 20-25mg/kg of 200mg/kg and intraperitoneal injection sub-threshold dose are shared, and mouse can be caused to fall asleep；It is sprayed respectively with sulphur Appropriate sodium (intraperitoneal injection, 40mg/kg), chloraldurate (intraperitoneal injection, 200mg/kg) share, can significant its CNS inhibition of reinforcement work With.Also spirit caused by central stimulant amphetamine (intraperitoneal injection, 4-6mg/kg) and caffeine (50mg/kg) can obviously be fought Move sexual excitement；Banlangen, not only without antagonism, makes its enhancing of fainting from fear to convulsions caused by strychnine and pentylenetetrazol instead With its animal dead number of increase；Banlangen exclusive use has slight analgesic activity, shares with threshold dose morphine, can dramatically increasing Add its percentage that eases pain.Anti-allergic effects: matrine can reduce the release of Anaphylactic mediator, be immunosuppressor, inhibit 50%T The concentration of cell Proliferation is 0.55-0.56mg/ml；The concentration for inhibiting IL-2 yield is 0.1mg/kg.Immunosuppressive action: kuh-seng Alkali, oxymatrine have inhibiting effect to immune function of mice under 1/5 LD50 dosage.Rabbit, rat muscle injection oxidation are bitter Ginseng alkali 150mg/kg or 100mg/kg has obvious inhibiting effect to cutaneous anaphylaxis is passively or actively, and to rabbit anteserum IgE Antibody is formed with obvious inhibiting effect.Be injected intraperitoneally oxymatrine 200mg/kgd, totally 21 days, to mouse immediate allergic Death caused by reacting has protective effect.Pharmacological action: 1. intramuscular injection oxymatrines can obviously fight croton oil, Irish moss The exudative inflammation that glue (rat) and glacial acetic acid (mouse) induce.Rabbit stomach-filling, subcutaneous injection, intravenous injection, intraperitoneal injection and flesh Meat injection kuh-seng decoction, lightyellow sophora root injection and matrine have diuresis, and sodium chloride amount has significant increase in urine.Kuh-seng 50% Methanol extract has ulcer function caused by anti-acidic alcohol, and active constituent is kurarinone (Kurarinone).In addition, kuh-seng There is antibacterial action in alkali inside and outside, and internal action intensity is suitable with chloramphenicol, and alcohol medicinal extract has an anti-trichomonal effect in vitro, intensity with Snake is close.Ginseng alkali toxicity: matrine is injected in rabbit, and discovery nervous centralis benumbs phenomenon, while spasm occurs, whole then exhale Inhale stop and it is dead.It is injected in frog, just in excitement, after then benumbing, breathing becomes slow and irregular, spasm finally occurs, with Cause breathing stop and it is dead；The breaking-out of its spasm is probably due to the hyperfunction of spinal reflex.Minimum lethal dose to rabbit is 0.4g/kg.Kuh-seng alkaloid plays the role of cold-blooded and warm-blooded animal to cause spasm and paralysis respiratory center.Receive larger dose There is the excited phenomenon such as dry jump, spasmodic tic in the mouse for measuring drug；Intravenous injection rabbit starts to occur fearing uneasiness, finally exhales It inhales difficult and dead.Sophocarpine causes venous congestion, blood pressure decline, exhales when in large dosage of (150mg/kg) injection rabbit body Inhale number significantly reduce, respiratory paralysis and be poisoned to death.Kuh-seng alkaloid LD50 measurement result report is inconsistent.Kuh-seng total flavone LD50 to mouse mainline is 103.1 ± 7.66g/kg.The LD50 of mouse muscle injection and intravenous injection oxymatrine Respectively 256.74 ± 573.6mg/kg and 144.2 ± 22.8mg/kg.The LD50 of stomach-filling and intramuscular injection kuh-seng medicinal extract mouse points It Wei not 14.5g/kg and 14.4g/kg.The LD50 of mouse subcutaneous injection kuh-seng crystalline alkali is 571.2 ± 48.8mg/kg dog muscle note Matrine knot prosperous (200mg/kg) or kuh-seng medicinal extract (0.1g/kg) are penetrated, dove intramuscular injection kuh-seng alkaloid (100mg/kg) is not See poisoning symptom.Kuh-seng contraceptive suppository is nonirritant to small white mouse vagina, uterine neck.

Tea polyphenols (Tea Polyphenols), meridian distribution of property and flavor: sweet and neutral.Major function: stomach of releiving is nervous, antidiarrheal and Diuresis.Pharmacological action: it 1. by adjusting the activity of immunoglobulin, realizes indirectly and improves human body comprehensive immunocompetence, antirheumatic The effect of factor, anti-bacteria and anti-virus.Tea polyphenols are stimulated by improving human immunoglobulin's total amount and it being made to maintain high level The variation of antibody activity, to improve the overall immune ability of people.It realizes indirectly and inhibits or kill various pathogen, germ and disease Effect of poison.2. antiallergic action and cutaneous anaphylaxis: the release action for the inhibition histamine that tea polyphenols can be strong, experiment card Bright tea polyphenols antiallergic action and anti-cutaneous anaphylaxis are 2 ~ 10 times stronger than the inhibitory effect of current common antiallergic.Tea is more The allergic conditions such as asthma are had significant treatment by the reflection of phenol energy inhibitory activity factor allergy as caused by antibody, adrenaline, enzyme etc. Effect.3. releive stomach anxiety, antidiarrheal and diuresis: tea polyphenols are reacted with stimulating gastrointestinal road, accelerate intestinal peristalsis to reach treatment The effect of constipation.Tea polyphenols inhibit and kill the various harmful cause of diseases for causing diarrhea by the combined immune ability of raising human body Bacterium, and the tense situation for stomach of releiving, to achieve the effect that anti-inflammatory antidiarrheal.Flavanols compounds being capable of stimulation of renal in tea polyphenols Vasodilation, increases the blood flow of kidney, to increase the filtration rate of glomerulus, excludes the lactic acid in urine.Human body Lactic acid in muscle, tissue is a kind of fatigue substances, and lactic acid, which excretes, can be such that the human body of fatigue is restored.4. promoting Vc Absorption, prevent and treat scurvy.Tea polyphenols can promote human body to ascorbic absorption, to effectively prevent and treat bad blood Disease.Other health-care efficacies: 1. anti peroxidation of lipid, it is pre- anti-aging.Tea polyphenols play the role of removing free radical, can inhibit skin Lipoxygenase and lipid peroxidation in mitochondria.Studies have shown that the antioxidant of tea polyphenols is substantially better than vitamin E, and with Injection Vitamin B_6 has synergy.2. the anti-solution to heavy metallic salt and biological alkalosis acts on: tea polyphenols to heavy metal have compared with Strong suction-operated can form complex compound with heavy metal and generate precipitating, be conducive to mitigate the murder by poisoning that heavy metal generates human body Effect.In addition, tea polyphenols also have the function of improving liver function and diuresis, thus there is preferable anti-solution to make biological alkalosis With.3. antiradiation injury, mitigate the adverse reaction of radiotherapy: tea polyphenols and its oxidation product have excellent radiation-resisting functional, can Radioactive substance is absorbed, prevents it from spreading in human body, referred to as natural ultraviolet filter.Tea polyphenols are as adjuvant treatment Means, can effectively maintain leucocyte, blood platelet, hemoglobin level stabilization；Improve bad anti-as caused by chemicotherapy It answers；The effective inhibiting effect alleviating ray and increasing weight to bone marrow cell；Effectively mitigate radiotherapy and chemotherapy medicine to human body immune system Inhibiting effect.4. preventing decayed tooth permanent tooth and the effect for removing halitosis: tea polyphenolic compounds can kill the cream present in slot Sour bacterium and other caries bacterias, have the function of inhibit glucose polymerisation enzymatic activity, such germ cannot on tooth implantation, have The process that the interruption of effect forms saprodontia.The protein food remained in slot becomes the matrix of putrefactivebacteria proliferation, tea Polyphenol can kill such bacterium.5. digestion-aid effect: gastral wriggling can be enhanced in tea polyphenols, prevents digestive organs disease The generation of disease.In addition tea polyphenols compound can be attached on the wound of stomach with filminess, be played a protective role to ulcer wound surface. 6. facilitating beauty and skin care: tea polyphenols are water-soluble substances, and the greasy of face can be removed by being washed one's face with it, restrain pore, have and disappear Poison, sterilizing, resisting age of skin reduce damage and other effects of the ultraviolet radiation in daylight to skin.Tea polyphenols can stop purple Outside line and the free radical for removing ultraviolet induction inhibit the formation of melanin to protect the normal function of melanocyte.Together When to lipid oxidation generate inhibition, mitigate pigmentation.

Cortex dictamni (psoralen), meridian distribution of property and flavor: bitter;It is salty;It is cold.Channel tropism: the spleen channel;Lung channel;Small intestinl channel;Stomach meridian;Bladder meridian. Major function: rubeola caused by wind-heat wet poison;Eczema;Scabies;Jaundice;Damp-heat arthralgia.Pharmacological action: antibacterial action: in vitro test The infusion of 1:4 has in various degree a variety of pathomycetes such as trichophyton, Trichophyton concentricum, oidium schoenleinii Inhibiting effect, but have report this product ether, ethyl alcohol and water extract to the coccuses such as golden yellow, Escherichia coli Pseudomonas aeruginosa and Mycobacterium graminis is showed no effect.1:20 decoction has no killing effect to trichomonas vaginalis.To the shadow of cardiovascular system and blood Ring: dictaminine contained by this product has excitation to isolated frog heart when a small amount of, cardiac muscular tension can be made to increase, minute output quantity and rich Output increases.There is apparent contraction to isolated rabbit ear blood vessel.Contained fagarine has antiarrhythmic effect, and bacterium taro alkali has The effect of ephedrine sample, can be such that anesthetized cat blood pressure rises, and enhancing cat instant embrane shrinks, expands coronary artery, enhance adrenergic boosting and make With.Antitumaous effect: eosin staining procedures are the result shows that this product non-polar solvent extract object and volatile oil have Anticancer Activity in vitro, from this Isolated Cen skin ketone in product ether extract, dictamine and a kind of colourless transparent liquid derived from volatile oil are its external anticancer Effective component, 45% concentration can kill ehrlich ascites cancer, S180 and U14 cell, and obakunone, limonin and β-paddy Sterol is invalid.Medicine (poison) is of science: the mixing compound of dictamine, skimmianine and fagarine can reduce adrenaline to cavy seminal vesicle Effect, skimmianine can make muscular paralysis, and mammal blood pressure progressive can be caused to decline because involving cardiac muscle.Skimmianine energy The tension for improving striated muscle, reinforces the excitability of spinal reflex, and trigonelline pharmacological activity is weak, but has report it in nerve in one's early years Muscle specimen can reduce the duration of nerve, then first reduce to the duration of muscle and increase afterwards, in addition, trigonelline has certain anticancer to make With 12.5mg/kg can make P388 leukemia mouse life extend 31%.

A kind of content and drug effect that the Chinese medicine compound prescription conditioning liquid for preventing and treating hand-foot-and-mouth disease is original with various Chinese herbal medicines of the present invention, Such as:

1. honeysuckle decoction is diluted to the concentration of 1:1280, the phagocytic function for promoting leucocyte is remained to.Mouse peritoneal injects gold and silver Flower injection, is also obviously promoted inflammatory cell phagocytic function.

2. dictamine and a kind of colourless transparent liquid derived from volatile oil are the effective component of its external anticancer, 45% Concentration can kill ehrlich ascites cancer, S180 and U14 cell.

3. tea polyphenolic compounds can kill the lactic acid bacteria present in slot and other caries bacterias, has and inhibit Portugal The effect of grape sugar polymerase activity, such germ cannot on tooth implantation, effectively interrupted make saprodontia formed process.It is residual The protein food in slot is stayed to become the matrix of putrefactivebacteria proliferation, tea polyphenols can kill such bacterium.

4. matrine, oxymatrine have inhibiting effect to immune function of mice under 1/5 LD50 dosage.Rabbit, rat Intramuscular injection oxymatrine 150mg/kg or 100mg/kg have obvious inhibiting effect to cutaneous anaphylaxis is passively or actively, And obvious inhibiting effect is formed with to rabbit anteserum IgE antibody.Be injected intraperitoneally oxymatrine 200mg/kgd, totally 21 days, to small Death caused by mouse anaphylactic type has protective effect.

A kind of Chinese medicine compound prescription conditioning liquid for preventing and treating hand-foot-and-mouth disease of the present invention is compounded with various Chinese herbal medicine fluids, such as:

1, the compound of Fructus Forsythiae and coptis root, radix scutellariae composition, In Vitro Bacteriostasis ratio are stronger when being applied alone Fructus Forsythiae.

2, Cortex Phellodendri antibiotic effective ingredient is jamaicin, therefore its pharmacological action is substantially similar to coptis root, but content is low compared with coptis root. In vitro test to staphylococcus aureus, pneumococcus, corynebacterium diphtheriae, Streptococcus viridans, shigella dysenteriae (except sonnei), Hemolytic streptococcus, meningococcus, comma bacillus, bacillus anthracis are effective or have stronger inhibiting effect；To hay bacillus, Bordetella pertussis, clostridium tetani also have inhibiting effect；To H37Rv, mycobacterium tuberculosis without direct repression, but bacterium can be made Number is reduced, but bacteriostasis is presented in when concentration is up to 1%.When making experimental therapy with cavy inoculation Mycobacterium hominis (H37Rv), mouth Clothes or the curative effect of injection are very poor.To the cavy of inoculation Mycobacterium bovis, when the hydrochloric acid crystal intramuscular injection of phellodendron extract, There is certain curative effect.Cortex Phellodendri take a favorable turn to the clinical symptoms of tuberculosis patient and x-ray inspection, and is better than coptis root.

In the second aspect of the present invention, the present invention provides the preparation method of the Chinese medicine compound prescription conditioning liquid.Chinese medicine of the invention Compound conditioning liquid can be prepared by the formulation method of this field routine.Chinese medicine compound prescription conditioning liquid of the present invention is preferably Chinese medicine external system Agent.The preparation method of preferred Chinese medicine compound prescription conditioning liquid, includes the following steps:

(1) the bulk pharmaceutical chemicals each component is extracted, obtains each component extracting solution；

(2) by each component extracting solution compound to get.

As currently preferred technical solution, in step (1), the extracting method is that water extracts or alcohol extracting.

As currently preferred technical solution, increases following steps between step (1) and step (2): being purified Processing.

As currently preferred technical solution, the purification process is ethanol precipitation, centrifugal process or chitosan clarification Method.

In the third aspect of the present invention, a kind of Chinese medicine compound prescription conditioning liquid is provided in the drug of preparation prevention and treatment hand-foot-and-mouth disease In application.Preferably, the drug of the prevention and treatment hand-foot-and-mouth disease is antibacterial medicines.

A kind of Chinese medicine compound prescription conditioning liquid for preventing and treating hand-foot-and-mouth disease of the present invention is with the ecosystem of various Chinese herbal medicine fluids pathogenic The destruction of bacterium loses its function, finally kills pathogenic bacteria or inhibits pathogenic bacteria.Plant formula of the present invention, safe and nontoxic pair It acts on, is non-stimulated.Meanwhile being collocated with each other by distinctive formula combination and weight, so that mutually coordinated between each taste Chinese herbal medicine Play synergistic effect.The weight ratio distribution of this clock ingredient rationally, is not only able to play the drug effect of itself, moreover it is possible to mutually promote, take Obtain better bactericidal effect.

A kind of Chinese medicine compound prescription conditioning liquid for preventing and treating hand-foot-and-mouth disease of the present invention can be by using ultrasonic ultrasonic delay line memory, using super Liquid germicide molecular structure is broken up and generates the elegant water mist for being full of liquid germicide of nature by acoustic bearing pressure.In addition in mist Release is largely full of to the anion of liquid germicide during change, is generated with medicine mist, the dust etc. floated in air following Physical reactions (1) hit deposition.(2) gravity sedimentation.(3) disperse deposits.(4) deposition is intercepted.(5) electrostatic attraction deposits, atomization Make the indoor processing without dead angle, while along with the Chinese medicine compound prescription conditioning liquid is experiment proves that can effectively inhibit Escherichia coli, gold Spreading unchecked wantonly for staphylococcus aureus and Candida albicans, is purified air, improves environment, send out hand-foot-and-mouth disease Raw probability is reduced.Low for equipment requirements, strong operability of the invention, preparation efficiency is high, the residence after being applicable in classroom, finishing The purification of room, the environment in the compartment of car and office and air can effectively prevent hand-foot-and-mouth disease.

Specific embodiment

Below by way of preparation embodiment, the invention will be further elaborated:

Embodiment 1

The Chinese medicine material matched using following weight: 15 grams of radix scutellariae, 15 grams of coptis root, 15 grams of Cortex Phellodendri, 3 grams of Fructus Forsythiae, peppermint fresh leaf 8 Gram, 2 grams of honeysuckle, 2 grams of Radix Glycyrrhizae, 3 grams of kuh-seng, 0.5 gram of tea polyphenols, 6 grams of cortex dictamni.

Above-mentioned Chinese medicine feed components are carried out water extraction (to add the water of 8 times of amounts every time, impregnate 1 hour, extract three times, water Extracting solution is concentrated into 1.08 ~ 1.12(80 DEG C of relative density) after, carry out purification process, purification process can be ethanol precipitation, Centrifugal process or chitosan fining process), obtain each component extracting solution.Each component extracting solution is mixed into compounding uniformly up to liquid system Agent.

Liquid preparation addition ultrasonic ultrasonic delay line memory is atomized, atomization makes the indoor processing without dead angle, while adding again Upper Traditional Chinese medicine bacteriostatic Alevaire effectively inhibits spreading unchecked wantonly for Escherichia coli, staphylococcus aureus and Candida albicans, makes air It is purified, improves environment, the probability that hand-foot-and-mouth disease occurs is reduced, room, sedan-chair after being applicable in classroom, finishing The purification in the compartment of vehicle and the environment of office and air.The usage amount of the conditioning liquid is every 100 square metres of sprays 50ml present invention Conditioning liquid, once a day, 3 days as one therapeutic course.

Embodiment 2

The Chinese medicine material matched using following weight: 20 grams of radix scutellariae, 20 grams of coptis root, 20 grams of Cortex Phellodendri, 5 grams of Fructus Forsythiae, dried peppermint leaf 12 Gram, 6 grams of honeysuckle, 5 grams of Radix Glycyrrhizae, 6 grams of kuh-seng, 2 grams of tea polyphenols, 8 grams of cortex dictamni.

After above-mentioned Chinese medicine feed components are carried out alcohol extracting, purification process is carried out, purification process can be ethanol precipitation Method, centrifugal process or chitosan fining process), obtain each component extracting solution.Each component extracting solution is mixed into compounding uniformly up to liquid Preparation.

Embodiment 3

The Chinese medicine material matched using following weight: 25 grams of radix scutellariae, 25 grams of coptis root, 25 grams of Cortex Phellodendri, 2 grams of Fructus Forsythiae, peppermint fresh leaf 9 Gram, 4 grams of honeysuckle, 2.4 grams of Radix Glycyrrhizae, 2 grams of kuh-seng, 0.6 gram of tea polyphenols, 5 grams of cortex dictamni.

Further confirm is made to beneficial effects of the present invention below by way of test example:

Test example 1

One, project: pH value measurement is examined；Total number of bacterial colonies, coliform, staphylococcus aureus, Pseudomonas aeruginosa, hemolytic Streptococcus, fungus colony sum；Bacteriostatic test (coliform, staphylococcus aureus, Candida albicans)

Two, checked object: the Chinese medicine compound prescription conditioning liquid of present invention prevention and treatment hand-foot-and-mouth disease.

Three, inspection result and evaluation:

(1), inspection result:

1, pH value measurement result

2, microbial contamination detects

Note: negative control nutrient agar, sand Bao Shi agar medium, physiological saline, SCDLP, dextrose bouillon, lactose cholate are equal Asepsis growth.

3, to the fungistatic effect of Escherichia coli (8099)

Note: positive controls average colony number is 2.31 x 10⁴cfu/mL；Negative control group: PBS, culture medium are raw without bacterium It is long.

4, to the fungistatic effect of staphylococcus aureus (ATCC 6538)

Note: positive controls average colony number is 2.45x 10⁴cfu/mL；Negative control group: PBS, culture medium are raw without bacterium It is long.

5, to the fungistatic effect of Candida albicans (ATCC 10231)

Note: positive controls average colony number is 1.02x 10⁴cfu/mL；Negative control group: PBS, culture medium are raw without bacterium It is long.

(2), conclusion:

Through detecting, the pH value that the present invention prevents and treats the Chinese medicine compound prescription conditioning liquid of hand-foot-and-mouth disease is 6.23 .Total number of bacterial colonies, large intestine Flora, staphylococcus aureus, Pseudomonas aeruginosa, hemolytic streptococcus, that fungus colony sum meets GB15979-2002 is disposable Use amenities sanitary standard.The product stoste acts on 2min and is averaged bacteriostasis rate greater than 99.9% to Escherichia coli, there is stronger suppression Bacterium effect；Effect 2min is averaged bacteriostasis rate greater than 99.9% to staphylococcus aureus, there is stronger bacteriostasis；2min pairs of effect Candida albicans be averaged bacteriostasis rate be 99.9%, have stronger bacteriostasis, hand-foot-and-mouth disease can be effectively prevented.

Claims

1. a kind of Chinese medicine compound prescription conditioning liquid for preventing and treating hand-foot-and-mouth disease, which is characterized in that it is mainly by the raw material of following parts by weight Medicine composition: 15-25 parts of radix scutellariae, 15-25 parts of coptis root, 15-25 parts of Cortex Phellodendri, 2-5 parts of Fructus Forsythiae, 8-12 parts of dried peppermint leaf, honeysuckle 2-6 Part, 2-5 parts of Radix Glycyrrhizae, 2-6 parts of kuh-seng, 0.5-2 parts of tea polyphenols, 5-8 parts of cortex dictamni.

2. the Chinese medicine compound prescription conditioning liquid of prevention and treatment hand-foot-and-mouth disease as described in claim 1, which is characterized in that mainly by following heavy The bulk pharmaceutical chemicals for measuring part form: 25 parts of radix scutellariae, 25 parts of coptis root, and 25 parts of Cortex Phellodendri, 2 parts of Fructus Forsythiae, 9 parts of dried peppermint leaf, 4 parts of honeysuckle, Radix Glycyrrhizae 2.4 parts, 2 parts of kuh-seng, 0.6 part of tea polyphenols, 5 parts of cortex dictamni.

3. Chinese medicine compound prescription conditioning liquid as claimed in claim 1 or 2, which is characterized in that during the Chinese medicine compound prescription conditioning liquid is Medicine external preparation.

4. the preparation method of Chinese medicine compound prescription conditioning liquid as described in any one of claims 1-3, which is characterized in that including following steps It is rapid:

(2) by each component extracting solution compound to get.

5. preparation method as claimed in claim 4, which is characterized in that in step (1), the extracting method is water extraction or alcohol It extracts.

6. preparation method as claimed in claim 4, which is characterized in that increase following step between step (1) and step (2) It is rapid: to carry out purification process.

7. preparation method as claimed in claim 6, which is characterized in that the purification process be ethanol precipitation, centrifugal process or Chitosan fining process.

8. a kind of Chinese medicine compound prescription conditioning liquid as described in claim any one of 1-3 answering in the drug of preparation prevention and treatment hand-foot-and-mouth disease With.

9. application as claimed in claim 8, which is characterized in that the drug of the prevention and treatment hand-foot-and-mouth disease is antibacterial medicines.