CN104285538B - A kind of processing method of evodia lepta seed and evodia lepta mating system - Google Patents
A kind of processing method of evodia lepta seed and evodia lepta mating system Download PDFInfo
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Abstract
The invention belongs to breeding plant field, be specifically related to a kind of processing method of evodia lepta seed and the mating system of evodia lepta. Method for treating seeds of the present invention can available energy be abolished the strong phenomenon of seed, break evodia lepta seed dormancy phase shortening sprout time. The present invention is by the optimization to seed collection, seed artificial treatment technology, whole ground, sowing, field management technology; not only easy and simple to handle, cost is low, the seedling breeding cycle is short; germinate fast; emergence rate is high; for the industrialization artificial breeding of realizing evodia lepta is established solid technical foundation, to improving evodia lepta breeding coefficient, protection wild resource; promote that the sustainable use of resource is significant, there is the economic outlook of popularization energetically.
Description
Technical field
The invention belongs to breeding plant field, be specifically related to a kind of processing method and three of evodia lepta seedPitch bitter mating system.
Background technology
Evodia lepta Melicopeptelefolia (Champ.exBenth.) Hartley, for the Rutaceae honey fruit of medicinal cornel belongs toPlant, is used as medicine to be dried stem, branch and band leaf spray. Evodia lepta begins to be loaded in " south of the Five Ridges gather medicinal herbs record ", to have another name calledThree fork hardships, little Huang are loose, Ji Gushu, three bifurcation hardships, three rifles, trident tiger etc., are wild plant resource,Be distributed in the provinces and regions such as Chinese Hainan, Guangdong, Guangxi, Fujian, Taiwan, Yunnan, mainly grow in lowIn the bushes of mountain sparse woods and knob.
Evodia lepta plant is shrub or dungarunga, common high 2-8m, complete stool bitter. Bark canescence,There is Long Circle hole skin. Ye Weisan counts compound leaf, to life; The long 3-10cm of petiole, base portion slightly swells; LeafletThe oval shape lanceolar of sheet, long 6-12cm, wide 2-6cm, papery, the blunt point of tip, full edge or irregularShallow wavy, bottle green above leaf, below yellow green, have gland point, petiolule is short. Umbrella room shape circular cone flowerOrder armpit is raw, and floral axis and bennet, at the beginning by pubescence, gradually come off after spending. Spend little, unisexuality, yellow-white,Slightly fragrance; Calyx drastic crack, extensively avette, be about 0.5mm; Petal is 4 lobes, and oval is to Long Circle,Long 1.5-2mm, has gland point; Male flower has stamen 4 lobes, and long compared with petal, filigree is linear, and flower pesticide ovum shape is longCircle, degeneration ovary is short and small; Female flower ovary is close by hair, and staminodium 4 lobes are short compared with petal, flower pesticideSterile. The normal 2-3 of Follicle radish fruit, rare 1 or 2, the dark yellow brown of exocarp, to bronzing, has newborn point; SeedBlack is glossy, and ovum shape is spherical.
Evodia lepta nature and flavor bitter cold, the effect that have heat-clearing, detoxify, dispel the wind, dehumidifies, is mainly used in controllingTreatment is controlled abscess of throat, treating rheumatic ostealgia, malaria, jaundice, eczema, dermatitis, traumatic injury and worm snake and is stungThe diseases such as wound. Also be the primary raw material medicine of famous Chinese patent drug SANJIU WEITAI KELI, Ganmaoling Granules etc. simultaneouslyMaterial, market demand huge and year by year increase. And in recent years because of artificial forest exploitation and state farm construction,Wild resource is seriously damaged, and imbalance between supply and demand is day by day serious, therefore need badly carry out evodia lepta scale,GAP technical research, promotes the industrialization of evodia lepta production of crude drugs, protects wild resource simultaneously.
Area, the evodia lepta medicinal material main product south of the Five Ridges, is wild product, under field conditions (factors) by seminal propagation, plantsThe sub-maturity period be early September to early October, but there is strong phenomenon in its seed, dormancy time reaches5-6 month, the therefore extremely low common less than 8% of germination percentage, this is also to cause evodia lepta to plant limited headWant reason. Chinese patent CN103168600A discloses a kind of method for culturing seedlings of evodia lepta, has introducedThe technology of using seedling-raising cup and adopting the method for overhead layering breeding to breed evodia lepta seed, butRest period of being still limited to evodia lepta seed long and method have that breeding coefficient is low, seedling cost is high,The problems such as growing-seedling period is long, the quick industrialization that is difficult to realize evodia lepta seedling is produced and is bred. Therefore asWhat is broken the dormancy of evodia lepta seed and then promotes germination, thereby by artificial propagation and cultivation tridentBitter industrialized development is to expand source and the output of medicinal material, to become evodia lepta the matter of utmost importance in the field of breeding.
Summary of the invention
For this reason, technical problem to be solved by this invention is to provide one can effectively break evodia lepta kindThe processing method of sub-rest period, and further provide a kind of germinate fast, emergence rate is high, can realize industryChange the evodia lepta mating system of producing.
For solving the problems of the technologies described above, the invention provides a kind of evodia lepta mating system, its feature existsIn, the method includes the steps of:
(1) soak: by the evodia lepta seed of collection mass concentration be 0.2 ?0.4% KMnO4SolutionSoak; After immersion, with clear water drip washing, and add ethyl acetate again to soak; Discard after ethyl acetate,Taking 35 ?at 40 DEG C mass concentration as the washing powder water soaking of 0.5-2%, and in solution, rub seed to plantingSublist skin becomes yellow-white or rough surface, rinses extremely neutral with clear water;
(2) storage: the thin river sand after the seed after immersion treatment and sterilizing is mixed and piles husky storage,Control temperature 20 ?30 DEG C of storages 50-60 days, control temperature 8 ?15 DEG C of storage 20-30 days, described storageIn process, control the husky water content of described heap and be 5 ?8%, piling height is no more than 40cm;
(3) cause: screen out thin river sand, preserve described storage seed after treatment, prior to seeding by instituteState seed be dipped in concentration be 180 ?cause in the PEG solution of 220g/L and process, and rinse with clear water,.
Further, the processing method of described evodia lepta seed, comprises the steps:
(1) soak: the KMnO that is 0.3% by mass concentration by the evodia lepta seed of collection4Solution soaksBubble; After immersion, with clear water drip washing, and add ethyl acetate again to soak; Discard after ethyl acetate, with35 ?mass concentration is 1% at 40 DEG C washing powder water soaking, and in solution, rub seed to seed epidermisBecome yellow-white or rough surface, rinse extremely neutral with clear water;
(2) storage: the thin river sand after the seed after immersion treatment and sterilizing is mixed and piles husky storage,Control 25 DEG C of storages of temperature 50-60 days, control 10 DEG C of storage 20-30 days of temperature, described storageThe husky water content of the described heap of middle control be 5 ?8%, piling height is no more than 40cm;
(3) cause: screen out thin river sand, preserve described storage seed after treatment, prior to seeding by instituteState seed and be dipped in initiation processing in the PEG solution that concentration is 200g/L, and rinse with clear water, to obtain final product.
The processing method of described evodia lepta seed, comprises the steps: in described step (2) instituteThe volume ratio of stating evodia lepta seed and described thin river sand be 1:2 ?4.
A kind of evodia lepta mating system, the method includes the steps of:
1) gather seed: gather evodia lepta fruit, dry in the shade, after pericarp cracking, take out seed;
2) seed treatment: according to claim 1 ?3 arbitrary described methods seed is processed; 3)Whole ground: select soil layer subacidity red-yellow soil deep, that fertility is higher, moisture is sufficient carry out weeding turn over,Airing sterilization, ridging bedding, and smooth furrow face;
4) sowing: make base manure with mixed organic manure, and by evodia lepta seed after treatment step (2)Uniform broadcasting, to seedbed, is got fine earth and is covered, and sprinkles water and keeps seedbed moistening;
5) field management: water to keep seedbed moistening depending on weather condition, when tool 3 can be opened during to true leafExhibition thinning final singling work, in the time that evodia lepta seedling enters fast growing period, selectively imposes composite fertilizer,And transplant in next year.
Described step 4) in, the total nutrient N+P of described mixed organic manure2O5+K2The mass fraction of O >=25%, organic mass fraction is >=15%.
Described step 4) in, the application rate of described evodia lepta seed be 7 ?9kg/ mu, and preferred 8kg/Mu.
Described step 5) in, in the nutrient of described composite fertilizer N:P:K be 10 ?20:10 ?20:10 ?20.
Described step 3) in, the furrow of described furrow face are high be 25 ?30cm, furrow face width 1.0 ?1.2m, furrow ditchWide 45 ?55cm.
Described evodia lepta seed gathered in early September to early October, and mid-January next yearStart sowing.
Technique scheme of the present invention has the following advantages compared to existing technology:
The method of processing evodia lepta seed of the present invention is used washing powder water logging after processing by ethyl acetateThe mode that bubble is rubbed, can abolish the strong phenomenon of seed, makes seed epidermis move back wax, increases seed coat permeableGas has been abandoned dangerous high concentrated sulfuric acid processing simultaneously, has safety and environmental protection, easy operating, one-tenthThe feature that this is cheap; Adopt the method for wet husky alternating temperature stratification simultaneously, can break evodia lepta seed dormancy,Make the seed dormancy time foreshorten to 90 days by 6 months under natural conditions; Further adopt prior to seedingAfter PEG causes, can improve and sprout regularity and shorten sprout time.
The method that evodia lepta of the present invention is bred, by seed collection, seed artificial treatment technology,The research of whole ground, sowing, field management technology, the biology of and dormancy strong for evodia lepta seedCharacteristic, through repetition test, analyzed seed collection period, seed artificial treatment method, sow closeThe impact on evodia lepta seedling breeding such as degree, and adopt appropriateness to strengthen the method for application rate, can effectively controlNursery weeds quantity processed is saved land resource and human cost simultaneously, breeds according to the inventive methodEvodia lepta, whole ground, sow, emerge after, the emergence rate of evodia lepta can reach more than 35%.
Evodia lepta seedling breeding method of the present invention, easy and simple to handle, cost is low, the seedling breeding cycle is short,For the industrialization artificial breeding of realizing evodia lepta is established solid technical foundation, to improving evodia lepta breedingCoefficient, protection wild resource, promotes that the sustainable use of resource is significant, compares existing skillIn art, the method for seedling-raising cup overhead layering breeding is also more easily promoted.
Experimental example
The different artificial processing method evodia lepta percentage of seedgermination experimental studies of experimental example 1
1 materials and methods
1.1 experiment material
Experiment material is the open country gathering in Heng Po town, Wuhua County, Growth in Meizhou City, Guangdong on September 15th, 2012Raw evodia lepta seed (fruit and seed pictorial diagram are shown in Fig. 1,2).
1.2 experimental technique
1.2.1 different agent treatment evodia lepta percentage of seedgermination experiments
After evodia lepta Fruit returns, dry in the shade, after pericarp cracking, take out seed, be divided at random 4 groups, everyGroup 1000g, with mass percentage concentration be 0.3%KMnO4After solution disinfection 15 minutes, take out with clearAfter water wash, use respectively following methods processing: (1) clear water is processed and soaked 10 minutes, (2) concentrated sulfuric acidSoak 10 minutes, clear water flushing is to neutral, and (3) benzinum soaks 10 minutes, during clear water rinses extremelyProperty, (4) ethyl acetate soaks 10 minutes, and clear water rinses to neutral. After each group seed treatment,The washing powder water soaking that is 1% with the mass fraction of 35-40 DEG C 24 hours is then rubbed kind in solutionTill son becomes yellow-white or rough surface to seed epidermis, make seed coat take off wax, strengthen seed permeableGas, to neutral, then adopts the mode of now broadcasting of now adopting with clear water flushing, the random selection 1000 of every processingGrain seed is placed in on the germinating bed of the identical soil property of nursery and carries out Seed germination (seeing Fig. 3), statistics kindSub-germinating time and germination percentage. Above-mentioned experiment in triplicate, is calculated average germination percentage.
1.2.2 the germination percentage of evodia lepta seed under different reserve temperatures
The seed of processing through 1.2.1 item method (4), after washing powder water treatment, rinses with clear waterExtremely neutral, be divided at random 5 groups, every group of 1000g, (seed and thin river sand are by volume in wet husky storage respectivelyThan mixing for 1:3, the husky water content that wets is 5%-8%, and piling height is no more than 40cm) in belowUnder temperature conditions: (1) is now adopted now and broadcast without storage, preserves 90 days (3) under (2) 25 DEG C of environmental conditionsUnder 10 DEG C of environmental conditions, preserve 90 days, under (4) 0 DEG C of environment, preserve 90 days, (5) 25 DEG C of environmental conditionsLower storage, after 60 days, is preserved 30 days under 10 DEG C of environmental conditions. After storage finishes, every processing is selected at randomSelecting 1000 seeds is placed in on the germinating bed of the identical soil property of nursery and carries out Seed germination, statistics seedGermination initial time and germination percentage. Above-mentioned experiment in triplicate, is calculated average germination percentage.
1.2.3PEG cause the impact on evodia lepta seed germination
Seed after 1.2.2 item method (5) storage, causes and processes with PEG before sowing: adoptPEG4000, establishes 100gL-1、150g·L-1、200g·L-1、250g·L-1、300g·L-1Totally 5 placesReason, seed is dipped in a certain amount of liquid initator, and sealing is processed 24 little in 20 DEG C of constant incubatorsTime, taking out seed, clear water is cleaned, and dries in order to sowing, causes the seed of processing as right not doAccording to. Every processing is random to be selected 1000 seeds to be placed in on the germinating bed of the identical soil property of nursery to send outBud experiment, statistics potentiality of seed and germination percentage, germinating energy during for sowing 15 days germination number account for and broadcastPlant the percentage of number. Above-mentioned experiment in triplicate, is calculated average germination percentage.
2 results and analysis
2.1 the percentage of seedgermination of different agent treatment
Under different disposal, percentage of seedgermination result is as shown in table 1. Result shows, the concentrated sulfuric acid, acetic acid secondEster, benzinum process that all can to abolish evodia lepta seed strong, improve seed water-permeable and air permeable, shorten kindSub-germinating time, improves percentage of seedgermination. But different agent treatment percentages of seedgermination difference to some extent, withGiven this and the security of practical operation ethyl acetate soaks the processing optimum of 10 minutes,, therefore selectsIt is suitable that ethyl acetate soaks 10 minutes be treated to.
Evodia lepta germination situation under the different agent treatment of table 1
The germination percentage of evodia lepta seed under 2.2 different reserve temperatures
The long-pending process of the wet sand bed of seed, under different reserve temperatures, percentage of seedgermination situation is as shown in table 2. KnotFruit demonstration, there is dormancy in evodia lepta seed, need be through 25 DEG C of wet sand beds after long-pending 60 days, alternating temperature is extremely10 DEG C of ambient storage 30 days, can significantly break seed dormancy, shorten dormancy time, improve germinationRate. And constant temperature storage is not remarkable to breaking seed dormant effect, under especially 0 DEG C of environment is stored,Percentage of seedgermination significantly declines, and after this and evodia lepta seed maturation in annual October, after naturally coming off, needsThrough off-season variations in temperature, until the natural phenomena that Second Year 3-4 month just sprouts is consistent, Er QielingApproximately 10 DEG C of the regional winter temperature mean temperatures in south, rare 0 DEG C of low temperature.
The germination of evodia lepta seed under the different holding conditions of table 2
2.3PEG causes the impact on evodia lepta seed germination
As can be seen from Table 3, PEG4000 can significantly promote evodia lepta seed germination after causing. ?100g·L-1-300g·L-1Under concentration is processed, raise with initiator concentration, the germinating energy of evodia lepta seedAll present with germination percentage the trend of falling after rising, with 200gL-1Concentration for the treatment of the best, compare control group,Potentiality of seed has improved 208.6.5%, and germination percentage has improved 34.6%. Result shows, debita spissitudoPEG4000, can promote the sprouting of evodia lepta seed, and improve and sprout regularity (seed germination figureSee Fig. 4).
Table 3 variable concentrations PEG4000 processes the germination of lower evodia lepta seed
Experimental example 2 Different Harvesting Time evodia lepta percentage of seedgermination experimental studies
1 materials and methods
1.1 experiment material
Experiment material is to gather as for Heng Po town, Wuhua County, Growth in Meizhou City, Guangdong 10 months September in 2012Wild evodia lepta seed, acquisition time is September 5, September 15, September 25 respectively.
1.1 experimental technique
After every batch of evodia lepta Fruit returns, dry in the shade, after pericarp cracking, take out seed, random mensuration kindSub-mass of 1000 kernel, viability. With mass percentage concentration be 0.3%KMnO4Solution soaks 15 minutes,,Other are processed with 1.2.2 (5) group, add up thousand grain weigth, viability after gathering, after planting systemMeter percentage of seedgermination, germination condition is identical with above-mentioned each experiment, and every batch is in triplicate, calculates averageGermination percentage.
2. results and analysis
The seed collection time on germination percentage to affect situation as shown in table 4. Result shows, picking time mistakeEarly (early September, when the firm maturation of seed), seed ghost rate is higher, and viability is lower, after being unfavorable forPhase germinates; Picking time is excessively late, although kernel maturing is higher, viability is strong, and germination percentage increases,But seed major part comes off, be unfavorable for collecting; While more than 1/2 cracking with fruit, gather the most suitable (9The middle ten days and the last ten days moon), both can ensure higher seed vigor, be unlikely to again seed and come off cannot gather completelyTo enough seeds.
The impact of table 4 Different Harvesting Time on evodia lepta percentage of seedgermination
The research of the different thickness of sowing evodia lepta of experimental example 3 breeding experiment
1 materials and methods
1.1 experiment material
Experiment material is the open country gathering in Heng Po town, Wuhua County, Growth in Meizhou City, Guangdong on September 15th, 2012Raw evodia lepta seed, with mass percentage concentration be 0.3%KMnO4Solution soaks 15-20 minute, takes outWith soaking 10 minutes with ethyl acetate under room temperature condition after clear water drip washing, discard liquid, then use 35-40 DEG CThe mass fraction washing powder water soaking that is 1% 24 hours, then in solution, rub seed to seedTill epidermis becomes yellow-white or rough surface, make seed coat take off wax, strengthen seed water-permeable and air permeable, useClear water rinses to neutral, and by volume for 1:3 mixes, wet husky water content is 8% with thin river sand,Piling height is no more than 40cm, under 25 DEG C of environmental conditions, preserves 60 days, under 10 DEG C of environmental conditions, preserves30 days, in order to sowing.
1.2 experiment places
This was tested in 2012, carried out in Heng Po town, the Meizhou City Wuhua County evodia lepta base of growing seedlings. ExperimentGround is located in 23 ° of 23' of north latitude, and 115 ° of 18' of east longitude belong to subtropical zone monsoon season, sample plot physical features is smooth,On the sunny side, irrigation and drainage are convenient, and the soil is porous, and soil fertility is medium, and previous crops is peanut. The bedding of whole ground, furrowFace width 1.0m, high 30cm furrow furrow width 50cm. Planting site soil physico-chemical property: content of organic matter 14.2g/kg,Full nitrogen 1.19g/kg, available nitrogen 103.60mg/kg, full phosphorus 0.89g/kg, rapid available phosphorus 26.30mg/kg,Full potassium 21g/kg, available potassium 135.00mg/kg.
1.3 experimental design
3 processing: M1 (broadcast sowing, application rate is 4kg/ mu) are established in experiment, and (application rate is 8kg/ to M2Mu), M3 (application rate is 12kg/ mu). Random district group is arranged, repeats for 3 times, and totally 9 communities, everyIndividual community area 10m2 (long 10m × wide 1m), surrounding plantation evodia lepta is made protection row.
1.4 cultivation management
Sow by 1.3 experimental designs, before sowing, under 20 DEG C of conditions, seed is dipped in to 200gL-1In PEG4000 solution, cause 24 hours, after initiation, seed is rinsed sowing well with clear water. Broadcast15-20 days after kind, has germination to emerge, and within 25-40 days, a large amount of evodia lepta seedlings are unearthed afterwards. Seedling goes outAfter soil, water depending on weather condition, keep seedbed moistening, carrying out before thinning final singling work, in 4The end of month, image data, recorded seedbed unit are number of weeds, seedling number and seedling early growth data. OtherField management measure is with local Production of Large Fields.
2. results and analysis
Under different thicknesss of sowing, the evodia lepta result of growing seedlings is as shown in table 5. Result shows, different sowings are closeUnder degree, seedling stage weeds in field number, seedling emergence number and seedling early growth situation difference. Strengthen thickness of sowingCan significantly suppress weeds in field growth, improve seedling in garden quantity, but the excessive rear seedling early growth mistake of densityClose, be unfavorable for seedling early growth and later stage thinning work. Therefore the sowing of the best when selected evodia lepta is grown seedlingsDensity is 8kg/ mu, emerge neater, and weeds in field is less.
According to the application rate of 8kg/ mu, described evodia lepta seed is bred, select with potted plant and fieldPlantation pattern, experimental result is shown in respectively shown in accompanying drawing 5 and 6, visible, the trident under this thickness of sowingBitter mating system, emerge neater, and weeds in field is less, is convenient to field management.
The impact that table 5 thickness of sowing is grown seedlings on evodia lepta
Brief description of the drawings
For content of the present invention is more likely to be clearly understood, below according to concrete reality of the present inventionTest example also by reference to the accompanying drawings, the present invention is further detailed explanation, wherein
Fig. 1 is evodia lepta fruit pictorial diagram in experimental example 1 of the present invention;
Fig. 2 is evodia lepta seed pictorial diagram in experimental example 1 of the present invention;
Fig. 3 is evodia lepta seed treatment experiment in experimental example 1 of the present invention;
Fig. 4 is evodia lepta seed germination figure in experimental example 1 of the present invention;
Fig. 5 is the potted plant seedling figure of the evodia lepta of 8kg/ mu thickness of sowing in experimental example 3 of the present invention;
Fig. 6 is the evodia lepta field plantation seedling figure of 8kg/ mu thickness of sowing in experimental example 3 of the present invention.
Detailed description of the invention
Embodiment 1
Gather wild evodia lepta seed, former planting in mid-September, 2012 in Heng Po town, Meizhou City Wuhua CountyThing is accredited as rutaceae evodia lepta Melicopeptelefolia through South China Botanical Garden Ye Huagu researcher(Champ.exBenth.) Hartley, after seed collection dries in the shade, used mass percentage concentration September 27For 0.3%KMnO4Solution soaks 15 minutes, takes out with using acetic acid second under room temperature condition after clear water drip washingEster soaks 10 minutes, discards liquid, the washing powder water soaking that is then 1% with the mass fraction of 39 DEG C24 hours, till then rubbing seed to seed epidermis become yellow-white or rough surface in solution,Make seed coat take off wax, strengthen seed water-permeable and air permeable, rinse extremely neutral with clear water; With thin river sand by volumeThan mixing for 1:3, wet husky water content is 7%, and piling height 35cm, 25 DEG C of environmental conditionsLower storage 60 days, preserves 30 days under 10 DEG C of environmental conditions, and duration of storage is regularly checked wet husky temperatureWith humidity, meet abnormal high temperature, need stir wet husky cooling watering in time and keep wet husky moistening, storage is to 86 days time, there is germination. Storage finish after in by the end of December, 2012, at Heng Po town, Wuhua County Yang Encun2 mu of sub-Ji row's planting site selection of lands, ridging bedding, artificial smooth furrow face, the high 27cm of furrow, furrow face width 1.1m, furrow furrow width 50cm. Be on January 5th, 2013 sowing date, and sowing fore worker is except sand, 20 DEG CUnder condition, seed is dipped in to 200gL-1In PEG4000 solution, cause 24 hours, after initiation,Seed is rinsed well with clear water, broadcasts sowing on seedbed by 8kg/ mu even density, and earthing 0.5cm, broadcastsRear employing equipment of sprinkler irrigation is sprinkled water to seedbed, keeps seedbed moistening, and stratification, had seed after approximately 18 daysEmerge successively, within 30 days, a large amount of seedlings are unearthed afterwards. Remove in time weeds in field seedling stage, during to April 2,Seedling tool 5 is to true leaf, and height of seedling 9cm starts thinning, and thinning density is 8 × 10cm, after thinning andTime water normal root water, meet hot weather after entering June and need set up shade regular trickle, treat seedling envelopeAfter row, remove sunshade net. After June, evodia lepta seedling enters fast growing period, can suitably imposeComposite fertilizer (N:P:K=15:15:15), executes as main taking thin, every mu of 5kg, and interval fertilising in 2 months 1 time,Topdress altogether 2 times, after fertilising, note trickle. To in March, 2014, more than plant height can reach 60cm,The qualified seedling of per mu yield can carry out transplanting work, grow seedlings in this way, more than can reach 40,000 strains.
Embodiment 2
Gather wild evodia lepta seed, former planting in early September, 2012 in Heng Po town, Meizhou City Wuhua CountyThing is accredited as rutaceae evodia lepta Melicopeptelefolia through South China Botanical Garden Ye Huagu researcher(Champ.exBenth.) Hartley, after seed collection dries in the shade, September 7 by mass percentage concentration was0.3％KMnO4Solution soaks 20 minutes, takes out with using ethyl acetate under room temperature condition after clear water drip washingSoak 10 minutes, discard liquid, the washing powder water soaking 24 that is then 1% with the mass fraction of 35 DEG CHour, till then rubbing seed to seed epidermis become yellow-white or rough surface in solution, makeSeed coat takes off wax, strengthens seed water-permeable and air permeable, rinses extremely neutral with clear water; With thin river sand by volumeFor 1:3 mixes, wet husky water content is 8%, and piling height 35cm, under 20 DEG C of environmental conditionsPreserve 60 days, under 8 DEG C of environmental conditions, preserve 30 days, duration of storage regularly checks that wet husky temperature is with wetDegree, meets abnormal high temperature, need stir wet husky cooling watering in time and keep wet husky moistening, and storage was to 86 daysTime have a germination. Storage finish after in by the end of December, 2012, in Asia, Yang En village, Heng Po town, Wuhua County2 mu of Ji row's planting site selection of lands, ridging bedding, artificial smooth furrow face, the high 25cm of furrow, furrow face width 1.2M, furrow furrow width 50cm. Be on January 5th, 2013 sowing date, and sowing fore worker is except sand, 20 DEG C of barsUnder part, seed is dipped in to 200gL-1In PEG4000 solution, cause 24 hours, after initiation,Seed is rinsed well with clear water, broadcasts sowing on seedbed by 7kg/ mu even density, and earthing 1cm, after broadcastingAdopt equipment of sprinkler irrigation to sprinkle water to seedbed, keep seedbed moistening, stratification, had seed land after approximately 15 daysContinuous emerging, within 40 days, a large amount of seedlings are unearthed afterwards. Remove in time weeds in field seedling stage, to April, and seedling tool5 pairs of true leaves, height of seedling 5cm, starts thinning, and thinning density is 8 × 10cm, waters in time normal root after thinningWater, meets hot weather after entering June and need set up shade regular trickle, treats seedling Feng Hanghou, removesFall sunshade net. After June, evodia lepta seedling enters fast growing period, can suitably impose composite fertilizer (N:P:K=15:15:15), execute as main taking thin, every mu of 5kg, interval fertilising in 2 months 1 time, topdresses 2 altogetherInferior, after fertilising, note trickle. To in March, 2014, more than plant height can reach 60cm, can carry outTransplanting work, grows seedlings in this way, more than the qualified seedling of per mu yield can reach 40,000 strains.
Embodiment 3
Gather wild evodia lepta seed in early October, 2012 in Heng Po town, Meizhou City Wuhua County, formerPlant is accredited as rutaceae evodia lepta Melicopeptelefo through South China Botanical Garden Ye Huagu researcherLia (Champ.exBenth.) Hartley is after seed collection dries in the shade, dense with quality percentage October 7Degree is 0.3%KMnO4Solution soaks 15 minutes, takes out with using acetic acid under room temperature condition after clear water drip washingEthyl ester soaks 10 minutes, discards liquid, the washing powder water logging that is then 1% with the mass fraction of 40 DEG CSteep 24 hours, till then rubbing seed to seed epidermis become yellow-white or rough surface in solution,Make seed coat take off wax, strengthen seed water-permeable and air permeable, rinse extremely neutral with clear water; With thin river sand by volumeThan mixing for 1:3, wet husky water content is 5%, and piling height 35cm, 30 DEG C of environmental conditionsLower storage 50 days, preserves 20 days under 15 DEG C of environmental conditions, and duration of storage is regularly checked wet husky temperatureWith humidity, meet abnormal high temperature, need stir wet husky cooling watering in time and keep wet husky moistening, storage is to 86 days time, there is germination. Storage finish after in by the end of December, 2012, at Heng Po town, Wuhua County Yang Encun2 mu of sub-Ji row's planting site selection of lands, ridging bedding, artificial smooth furrow face, the high 30cm of furrow, furrow face width 1.0m, furrow furrow width 50cm. Be on January 5th, 2013 sowing date, and sowing fore worker is except sand, 20 DEG CUnder condition, seed is dipped in to 200gL-1In PEG4000 solution, cause 24 hours, after initiation,Seed is rinsed well with clear water, broadcasts sowing on seedbed by 9kg/ mu even density, and earthing 0.5cm, after broadcastingAdopt equipment of sprinkler irrigation to sprinkle water to seedbed, keep seedbed moistening, stratification, had seed land after approximately 20 daysContinuous emerging, within 25 days, a large amount of seedlings are unearthed afterwards. Remove in time weeds in field seedling stage, during to April 2,Seedling tool 5 is to true leaf, and height of seedling 10cm starts thinning, and thinning density is 5 × 10cm, after thinning andTime water normal root water, meet hot weather after entering June and need set up shade regular trickle, treat seedling envelopeAfter row, remove sunshade net. After June, evodia lepta seedling enters fast growing period, can suitably imposeComposite fertilizer (N:P:K=15:15:15), executes as main taking thin, every mu of 5kg, and interval fertilising in 2 months 1 time,Topdress altogether 2 times, after fertilising, note trickle. To in March, 2014, more than plant height can reach 60cm,The qualified seedling of per mu yield can carry out transplanting work, grow seedlings in this way, more than can reach 40,000 strains.
Obviously, above-described embodiment is only for example is clearly described, and not to the side of enforcementThe restriction of formula. For those of ordinary skill in the field, also can on the basis of the above descriptionTo make other changes in different forms. Here without also giving all embodimentsWith exhaustive. And the apparent variation of being extended out thus or the still guarantor in the invention of variationProtect among scope.
Claims (12)
1. a processing method for evodia lepta seed, is characterized in that, comprises the steps:
(1) soak: the KMnO that is 0.2-0.4% by mass concentration by the evodia lepta seed of collection4MoltenImmersion bubble; After immersion, with clear water drip washing, and add ethyl acetate again to soak; Discard after ethyl acetate,Washing powder water soaking taking mass concentration at 35-40 DEG C as 0.5-2%, and in solution, rub seed to plantingSublist skin becomes yellow-white or rough surface, rinses extremely neutral with clear water;
(2) storage: the thin river sand after the seed after immersion treatment and sterilizing is mixed and piles husky storage,First control temperature 20-30 DEG C storage 50-60 days, again control temperature 8-15 DEG C storage 20-30 days, described inIn storage, controlling the husky water content of described heap is 5-8%, and piling height is no more than 40cm;
(3) cause: screen out thin river sand, preserve described storage seed after treatment, prior to seeding by instituteState seed and be dipped in initiation processing in the PEG solution that concentration is 180-220g/L, and rinse with clear water,.
2. the processing method of evodia lepta seed according to claim 1, is characterized in that, comprisesFollowing steps:
(1) soak: the KMnO that is 0.3% by mass concentration by the evodia lepta seed of collection4Solution soaksBubble; After immersion, with clear water drip washing, and add ethyl acetate again to soak; Discard after ethyl acetate, withThe washing powder water soaking that at 35-40 DEG C, mass concentration is 1%, and in solution, rub seed to planting sublistSkin becomes yellow-white or rough surface, rinses extremely neutral with clear water;
(2) storage: the thin river sand after the seed after immersion treatment and sterilizing is mixed and piles husky storage,First control 25 DEG C of temperature storage 50-60 days, control 10 DEG C of storage 20-30 days of temperature, described storage againIn process, controlling the husky water content of described heap is 5-8%, and piling height is no more than 40cm;
(3) cause: screen out thin river sand, preserve described storage seed after treatment, prior to seeding by instituteState seed and be dipped in initiation processing in the PEG solution that concentration is 200g/L, and rinse with clear water, to obtain final product.
3. the processing method of evodia lepta seed according to claim 1 and 2, is characterized in that,Comprise the steps: in described step (2) volume ratio of described evodia lepta seed and described thin river sandFor 1:2-4.
4. an evodia lepta mating system, is characterized in that, the method includes the steps of:
1) gather seed: gather evodia lepta fruit, dry in the shade, after pericarp cracking, take out seed;
2) seed treatment: seed is processed according to the arbitrary described method of claim 1-3;
3) wholely: select soil layer subacidity red-yellow soil deep, that fertility is higher, moisture is sufficient to removeGrass is turned over, airing is sterilized, ridging bedding, and smooth furrow face;
4) sowing: make base manure with mixed organic manure, and by evodia lepta seed after treatment step (2)Uniform broadcasting, to seedbed, is got fine earth and is covered, and sprinkles water and keeps seedbed moistening;
5) field management: water to keep seedbed moistening depending on weather condition, when tool 3 can be opened during to true leafExhibition thinning final singling work, in the time that evodia lepta seedling enters fast growing period, selectively imposes composite fertilizer,And transplant in next year.
5. evodia lepta mating system according to claim 4, is characterized in that, described step 4)In, the total nutrient N+P of described mixed organic manure2O5+K2Mass fraction >=25% of O, organic matterAmount mark is >=15%.
6. evodia lepta mating system according to claim 5, is characterized in that, described step 4)In, the application rate of described evodia lepta seed is 7-9kg/ mu.
7. according to the evodia lepta mating system described in claim 4-6 any one, it is characterized in that instituteState step 5) in, in the nutrient of described composite fertilizer, N:P:K is 10-20:10-20:10-20.
8. according to the evodia lepta mating system described in claim 4-6 any one, it is characterized in that instituteState step 3) in, the furrow height of described furrow face is 25-30cm, furrow face width 1.0-1.2m, furrow furrow width 45-55cm.
9. evodia lepta mating system according to claim 7, is characterized in that, described step 3)In, the furrow height of described furrow face is 25-30cm, furrow face width 1.0-1.2m, furrow furrow width 45-55cm.
10. according to the evodia lepta mating system described in claim 4-6 any one or 9, it is characterized in that,Described evodia lepta seed gathered in early September to early October, and in mid-January next yearSowing.
11. evodia lepta mating systems according to claim 7, is characterized in that described tridentBitter seed gathered in early September to early October, and started sowing mid-January next year.
12. evodia lepta mating systems according to claim 8, is characterized in that described tridentBitter seed gathered in early September to early October, and started sowing mid-January next year.
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| KR102220918B1 (en) * | 2019-10-22 | 2021-02-26 | 성균관대학교산학협력단 | Pharmaceutical Composition comprising Euodia pasteuriana extract for Preventing or Treating Inflammatory disease as active ingredient |
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