CN104257808B - A kind of Tibetan medicine for the treatment of constipation - Google Patents
A kind of Tibetan medicine for the treatment of constipation Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/74—Rubiaceae (Madder family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
- A61K33/14—Alkali metal chlorides; Alkaline earth metal chlorides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/47—Euphorbiaceae (Spurge family), e.g. Ricinus (castorbean)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/55—Linaceae (Flax family), e.g. Linum
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/71—Ranunculaceae (Buttercup family), e.g. larkspur, hepatica, hydrastis, columbine or goldenseal
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
Abstract
The invention discloses a kind of Tibetan medicine for the treatment of constipation, it is made up of active component or is made up of active component and pharmaceutically acceptable adjuvant, and wherein said active component is prepared from according to a certain weight ratio by ZANGQIANCAO, Sal Ammoniacus, Tronae, sausage fruit, Herba Oxytropis Kansuensis, Folium Paraquilegiae microphyllae, Semen Sesami Nigrum, Caulis et Folium Lini, Semen Ricini.It can be prepared to the conventional dosage form for oral administration of any one.Tibetan medicine of the present invention has effect of the knot that purges heat clearly, the broken stagnant painful abdominal mass that disappears.The diseases such as mass in the abdomen, constipation, belch is suffered from abdominal pain, gastrointestinal function weakens are tied for gastrointestinal is long-pending.
Description
Technical field
The present invention relates to a kind of Tibetan medicine for the treatment of constipation, belong to Tibetan medicine field.
Background technology
Constipation is one group of different substantiality disease, main manifestations for drain knot just, defecation effort and defecation frequency reduce.Constipation is the common disease caused by Different types of etiopathogenises, as gastroenteropathy, accumulative gastral systemic disease, many medicines also can cause constipation.
Habitual constipation refers to long-term, chronic functional constipation, is mainly in old people.Be clinical common chronic digestible road symptom, main manifestations is defecation frequency minimizing, difficult defecation or hard excrement.Be common in constitutional Intestinal motility abnormal, the conveying of stool wriggling delays or megacolon etc.Result from that anxiety, pressure are large, enterogastric peristalsis imbalance more, or have awareness of defecation to bear just, form vicious cycle, cause habitual constipation.
Though itself be not a kind of disease, but be found in various diseases, along with the change of dietary structure, the impact of psychiatric and social factor, the sickness rate of constipation rises gradually, have a strong impact on the quality of life of people, and play an important role in the generation that colon cancer, hepatic encephalopathy, mastopathy, presenile dementia etc. are sick, when the disease such as acute myocardial infarction, cerebrovas-cularaccident, constipation can cause life unexpected.Therefore early prevention and rational therapy constipation, will the serious consequence brought of alleviate constipation and burden on society greatly.
Although this disease is very harmful, but not yet have better solution at present, the medicine of Western medicine, Chinese medicine, collaborative treatment of Chinese and western medicines constipation is also a lot, and has obtained certain curative effect, but still have some patients to conventional emplastic unsatisfactory curative effect, or can not apply because of its side effect; And Tibetan medicine treatment constipation, then there is the advantages such as successful, side effect be little.
Therefore, there is tight demand to the Tibetan medicinal preparation that curative effect better treats constipation in people.Up to now, any report about Tibetan medicinal composition of the present invention and preparation method thereof is not also found.The present inventor through repeatedly studying, and by the checking repeatedly of animal and clinical trial, finally have found Tibetan medicine oral drugs of the treatment constipation of better curative effect and preparation method thereof, thus completes the present invention.
Summary of the invention
The object of the invention is just to provide a kind of Tibetan medicine of more effectively treating constipation.
The present invention is a kind of Tibetan medicine, be be made up of active component or be made up of active component and pharmaceutically acceptable adjuvant, wherein said active component is made up of following bulk drugs: ZANGQIANCAO, Sal Ammoniacus, Tronae, sausage fruit, Herba Oxytropis Kansuensis, Folium Paraquilegiae microphyllae, Semen Sesami Nigrum, Caulis et Folium Lini, Semen Ricini.
It has selected ZANGQIANCAO, Sal Ammoniacus, Tronae, sausage fruit, Herba Oxytropis Kansuensis, Folium Paraquilegiae microphyllae, Semen Sesami Nigrum, Caulis et Folium Lini, Semen Ricini carry out combining as crude drug, and wherein (1) ZANGQIANCAO is Maguireothamnus speciosus light stem Radix Rubiae RubiawallichianaDecne. and Tibet Radix Rubiae R.tibeticaHook.f. and the dry root and rhizome belonging to several plants together.There is effect of clearing away heat and cooling blood.For disorders of blood, diffusion is got mildewed in warm conditions, and lung kidney-heat is evil, intestine and small intestine heat.(2) Sal Ammoniacus is halogenide class halite race halite, main sodium chloride-containing (NaCl).There is stomach warming relieving constipation, effect of relieving distension.For the complication of " Baconic " and " dragon ", abdominal distention borborygmus, constipation, choke,hiccup and regurgitation.(3) Tronae is Sulfates nahcolite scale race mineral trona, main containing sodium carbonate (Na
2cO
3).There is removing toxic substances and promoting pus discharge, eliminate indigestion and phlegm, the effect of anthelmintic relieving constipation.For Baconic's flatulence, dyspepsia, skin infection, parasitosis, toxic hepatitis, defecates unfavorable.(4) sausage fruit is the dry mature fruit of leguminous plant Cassia fistula L. CassiafistulaL..There is removing heat from the liver, removing toxic substances, detumescence, the effect captured. for hepatitis, liver poisoning, constipation, limb edema.(5) Herba Oxytropis Kansuensis is the dry herb of leguminous plant Herba Oxytropis falcatae OxytropisfalcataBunge and Herba Oxytropis chiliophyllae O.chiliophyllaRoyle.Have heat-clearing and toxic substances removing, granulation promoting heals skin ulcer, effect of hesitant pulse hemostasis, relieving constipation.Be used for the treatment of epidemic disease, toxonosis, grasserie, constipation, anthrax.(6) Folium Paraquilegiae microphyllae is the dry aerial parts that ranunculaceae plant intends Aquilegia viridiflora Paraquilegiamicrophylla (Royle) Drumm.etHutch..There is blood stasis removing, hemostasis, analgesia, the effect of hastening parturition.For traumatic injury, placenta retention, lower stillborn fetus.(7) Semen Sesami Nigrum is the dry mature seed of Pedaliaceae plant sesame SesamumindicumL..There is invigorating the liver and kidney, benefiting essence-blood, effect that intestine moistening is dry.For dizziness and blurred vision, Hiccough and deaf, early whitening of beard and hair, post disease alopecia, dryness of the intestine constipation.(8) Caulis et Folium Lini is the dry mature seed of flax family (Linaceae) plant Caulis et Folium Lini LinumusitatissimumL..Have and moisturize, the effect of dispeling the wind.For dryness of the intestine constipation, xerosis cutis pruritus, hair is withered to come off.(9) Semen Ricini is the seed of euphorbia plant Semen Ricini RicinuscommunisL..There is loosening bowel to relieve constipation, emetic, rush down effect of poison fire.For dyspepsia, nosotoxicosis, imperial, red bar, Baconic lack of proper care the syndrome caused.These crude drug are combinationally used and makes each crude drug effect produce synergism, thus effectively can treat constipation.
The consumption of Tibetan medicine active component of the present invention is also through inventor and gropes in a large number to sum up and draw, each crude drug consumption for all to have good therapeutic effect within the scope of following concrete weight proportion:
ZANGQIANCAO 20 ~ 60g, Sal Ammoniacus 15 ~ 45g, Tronae 15 ~ 45g, sausage fruit 25 ~ 75g, Herba Oxytropis Kansuensis 15 ~ 45g, Folium Paraquilegiae microphyllae 12.5 ~ 37.5g, Semen Sesami Nigrum 10 ~ 30g, Caulis et Folium Lini 7.5 ~ 22.5g, Semen Ricini 5 ~ 15g.
Be preferably: ZANGQIANCAO 32 ~ 48g, Sal Ammoniacus 24 ~ 36g, Tronae 24 ~ 36g, sausage fruit 40 ~ 60g, Herba Oxytropis Kansuensis 24 ~ 36g, Folium Paraquilegiae microphyllae 20 ~ 30g, Semen Sesami Nigrum 16 ~ 24g, Caulis et Folium Lini 12 ~ 18g, Semen Ricini 8 ~ 12g.
More preferably: ZANGQIANCAO 40g, Sal Ammoniacus 30g, Tronae 30g, sausage fruit 50g, Herba Oxytropis Kansuensis 30g, Folium Paraquilegiae microphyllae 25g, Semen Sesami Nigrum 20g, Caulis et Folium Lini 15g, Semen Ricini 10g.
The preparation of Tibetan medicine active component of the present invention can be pulverized by the crude drug convection drying of above-mentioned consumption to make; Also the crude drug of above-mentioned consumption can be adopted the conventional method of Chinese medicine preparation such as decoction and alcohol sedimentation technique or ethanol extract from water precipitation (see " pharmaceutics of Chinese drugs " 73rd ~ 74 pages of Cao Chunlin chief editor, Science and Technology of Shanghai publishing house publishes in November, 1986) to obtain.
The active component of Tibetan medicine of the present invention can add various customary adjuvant required when preparing different dosage form, as disintegrating agent, lubricant, binding agent etc. with the method for Chinese medicinal of routine (see Cao Chunlin chief editor " pharmaceutics of Chinese drugs ", Science and Technology of Shanghai publishing house in November, 1986 publishes) be prepared into any one and commonly use peroral dosage form, as powder, pill, capsule, granule, tablet etc.
Tibetan medicine of the present invention has effect of the knot that purges heat clearly, the broken stagnant painful abdominal mass that disappears.The diseases such as mass in the abdomen, constipation, belch is suffered from abdominal pain, gastrointestinal function weakens are tied for gastrointestinal is long-pending.
The usage and dosage of Tibetan medicine of the present invention is: oral; A 1 ~ 1.5g, 1 ~ 3 time on the one.
[detailed description of the invention]
Carry out the preparation method setting forth Tibetan medicine of the present invention further by the following examples.
The preparation of [embodiment 1] Tibetan medicine powder of the present invention:
Take ZANGQIANCAO 40g, Sal Ammoniacus 30g, Tronae 30g, sausage fruit 50g, Herba Oxytropis Kansuensis 30g, Folium Paraquilegiae microphyllae 25g, Semen Sesami Nigrum 20g, Caulis et Folium Lini 15g, Semen Ricini 10g, be jointly ground into fine powder after mixing, mixing, subpackage, obtains powder.
The preparation of [embodiment 2] Tibetan medicine pill of the present invention:
Take ZANGQIANCAO 20g, Sal Ammoniacus 15g, Tronae 15g, sausage fruit 25g, Herba Oxytropis Kansuensis 15g, Folium Paraquilegiae microphyllae 12.5g, Semen Sesami Nigrum 10g, Caulis et Folium Lini 7.5g, Semen Ricini 5g, be jointly ground into fine powder after mixing, mixing, with water pill, dry below 60 DEG C, polishing, packaging, obtains pill.
The preparation of [embodiment 3] Tibetan medicine granule of the present invention:
Take ZANGQIANCAO 60g, Sal Ammoniacus 45g, Tronae 45g, sausage fruit 75g, Herba Oxytropis Kansuensis 45g, Folium Paraquilegiae microphyllae 37.5g, Semen Sesami Nigrum 30g, Caulis et Folium Lini 22.5g, Semen Ricini 15g, jointly fine powder is ground into after mixing, mixing, add adjuvant and make granule, dry below 60 DEG C, granulate, subpackage, obtains granule.
The preparation of [embodiment 4] Tibetan medicine capsule of the present invention:
Take ZANGQIANCAO 32g, Sal Ammoniacus 24g, Tronae 24g, sausage fruit 40g, Herba Oxytropis Kansuensis 24g, Folium Paraquilegiae microphyllae 20g, Semen Sesami Nigrum 16g, Caulis et Folium Lini 12g, Semen Ricini 8g, be jointly ground into fine powder after mixing, mixing, load gelatine capsule, obtain capsule.
The preparation of [embodiment 5] Tibetan medicine tablet of the present invention:
Take ZANGQIANCAO 48g, Sal Ammoniacus 36g, Tronae 36g, sausage fruit 60g, Herba Oxytropis Kansuensis 36g, Folium Paraquilegiae microphyllae 30g, Semen Sesami Nigrum 24g, Caulis et Folium Lini 18g, Semen Ricini 12g, be jointly ground into fine powder after mixing, mixing, add adjuvant and make granule, dry below 60 DEG C, granulate, tabletting, obtains tablet.
Set forth the beneficial effect of Tibetan medicine of the present invention further below by way of test example, these test examples include pharmacodynamics test and the clinical observation on the therapeutic effect test of Tibetan medicine embodiment 1 powder of the present invention.
The test such as defecation, Small intestine advance activity, large intestine movement, the impact of intestinal volume, functions of loosening bowel relieving constipation of [test example 1] Tibetan medicine embodiment 1 powder of the present invention:
Test material: anthology invention Tibetan medicine embodiment 1 powder is trial drug; DAHUANG TONGBIAN JIAONANG is produced by Jianjiang Pharmaceutical Factory, batch number 20110712; India ink is produced by Xi Zhong chemical plant, Beijing, batch number 20110808; Compound diphenoxylate is produced by Jiaozuo City Kang Li Pharmaceutical limited company, batch number 20111002; Pentobarbital sodium, SERVA import subpackage, chemical plant product is known in upper sea-run, batch number 20110314; Kunming mouse, Wistar rat, provided by Lanzhou Experimental Animal Center.
Experimental technique: 1, Tibetan medicine embodiment 1 powder of the present invention is tested the defecation of dryness accumulated in the stomach and intestine dehydration Constipation Model mice: get body weight 24 ~ 26g healthy mice, male and female half and half, 6 groups are divided at random by body weight, if blank group, model control group, positive controls (DAHUANG TONGBIAN JIAONANG 0.5g/Kg) and Tibetan medicine embodiment 1 powder (1.5g/kg of the present invention, 3g/kg, 6g/kg) basic, normal, high dosage group.Except blank group, other are respectively organized mice and prohibit water non-fasting 72h, cause dryness accumulated in the stomach and intestine dehydration Constipation Model.Blank group gavage gives the 2% india ink suspension be configured to distilled water, administration group then gavage gives the 2% india ink suspension replacing distilled water with medicinal liquid, administration volume 1mL/20g body weight, after administration, mice is put into mice box, 1, every box, the clean filter paper of underlay white, observed and recorded every mice discharges the melena sum that mice in time (min) of melena and 4h is discharged first.T checks the significance of each group difference, the results are shown in Table 1.
Table 1 Tibetan medicine embodiment 1 of the present invention powder is on the impact (x ± s, n=10) of dryness accumulated in the stomach and intestine dehydration Constipation Model mice defecation
Group Animals number dosage (g/kg) is arranged row's melena in melena time (min) 4h first and is counted
Blank group 10 194.6 ± 67.52.4 ± 1.6
Model control group 10 358.8 ± 15.4
#0.3 ± 1.8
#
Positive controls 100.5325.4 ± 61.40.7 ± 1.6
Low dose group 101.5337.8 ± 63.80.5 ± 1.7
Middle dosage group 103269.2 ± 79.3
*1.8 ± 2.6
*
High dose group 106231.1 ± 68.2
*2.9 ± 2.4
*
Illustrate: compare with blank group:
#p<0.01; Compare with model control group:
*p<0.05;
*p<0.0l.
2, Tibetan medicine embodiment 1 powder of the present invention is tested the defecation of compound diphenoxylate (DC) model mice: animal and grouping are with 1, each group of mice prohibits water non-fasting 12h, except blank group is to except equivalent distilled water, all the other are respectively organized gavage and give compound diphenoxylate (DC) suspension 50mg/kg.After 30min, each treated animal gavage gives test medicine, and administration volume 1mL/20g body weight, blank group and model control group are to equivalent distilled water.Observed and recorded often organizes that the argol that mice discharges is counted, loose stool is counted and the number of animals of not defecation, and Continuous Observation 12h, respectively organizes index, and t checks the significance of each group difference, the results are shown in Table 2.
Table 2 Tibetan medicine embodiment 1 of the present invention powder is on the impact (x ± s, n=10) of DC Constipation Model mice defecation
Group Animals number dosage (g/kg) argol loose stool of counting is counted not defecation number of animals
Blank group 10 8.4 ± 4.2800
Model control group 10 2.6 ± 2.52
#00
Positive controls 100.53.9 ± 1.2800
Low dose group 101.54.1 ± 3.630.3 ± 0.270
Middle dosage group 1034.7 ± 2.58
*0.5 ± 0.230
High dose group 1069.4 ± 3.34
*1.9 ± 1.45
*0
Illustrate: compare with blank group:
#p<0.01; Compare with model control group:
*p<0.05;
*p<0.0l.
3, Tibetan medicine embodiment 1 powder of the present invention is tested the defecation of normal mouse: get body weight 24 ~ 26g healthy mice, male and female half and half, 5 groups are divided at random by body weight, if blank group, positive controls (DAHUANG TONGBIAN JIAONANG 0.5g/Kg) and Tibetan medicine embodiment 1 powder (1.5g/kg of the present invention, 3g/kg, 6g/kg) basic, normal, high dosage group.Blank group gavage gives the 2% india ink suspension be configured to distilled water, and administration group then gavage gives to replace the 2% india ink suspension that is configured to of distilled water with medicinal liquid, and administration volume is 1mL/20g body weight.After administration, mice is put into mice box, 1, every box, the clean filter paper of underlay white, observed and recorded every mice discharges the sum of mice discharge melena in time (min) of melena and 4h first.T checks the significance of each group difference, the results are shown in Table 3.
Table 3 Tibetan medicine embodiment 1 of the present invention powder is on the impact (x ± s, n=10) of normal mouse defecation
Group Animals number dosage (g/kg) is arranged row's melena in melena time (min) 4h first and is counted
Blank group 10 151.3 ± 73.42.6 ± 2.46
Positive controls 100.5119.7 ± 43.67.2 ± 2.26
*
Low dose group 101.5143.6 ± 54.84.5 ± 1.43
Middle dosage group 103116.4 ± 47.55.6 ± 2.38
*
High dose group 10698.6 ± 42.3
*8.7 ± 2.87
*
Illustrate: compare with model control group:
*p<0.05;
*p<0.0l.
4, Tibetan medicine embodiment 1 powder of the present invention is tested mouse small intestine ahead running: animal and grouping are with 3, take carbon ink as mark, blank group gavage gives the 7% carbon ink suspension be configured to normal saline, administration group then gavage gives to replace the 7% carbon ink suspension that is configured to of distilled water with medicinal liquid, administration volume is 0.4mL/20g body weight, 30min after gavage, cervical dislocation puts to death mice, to cut open the belly immediately taking-up gastrointestinal, be laid on glass plate, measure displacement in intestinal tube of carbon ink head end and small intestinal total length (from pylorus to ileum), calculate ink propulsive rate, distance/small intestinal total length × 100% of carbon ink propelling rate=carbon ink front end and pylorus.T checks the significance of each group difference, the results are shown in Table 4.
The impact (x ± s, n=10) that table 4 Tibetan medicine embodiment 1 of the present invention powder advances small intestine movement of mice
Group Animals number dosage (g/kg) small intestinal length (cm) carbon ink advance distance (cm) ink propulsive rate (%)
Blank group 10 53.8 ± 2.3442.5 ± 3.3278.17 ± 6.38
Positive controls 100.549.9 ± 4.1844.6 ± 6.4691.42 ± 12.08
*
Low dose group 101.548.8 ± 5.1737.8 ± 6.2779.16 ± 9.64
Middle dosage group 10349.6 ± 3.1543.2 ± 6.7888.73 ± 9.53
*
High dose group 10649.1 ± 4.1647.9 ± 5.38
*96.65 ± 6.72
*
Illustrate: compare with model control group:
*p<0.05;
*p<0.0l.
5, Tibetan medicine embodiment 1 powder of the present invention is to the large intestine movement test of rat: get body weight 250 ~ 350g healthy adult rat, all male, 5 groups are divided at random by body weight, if blank group, positive controls (DAHUANG TONGBIAN JIAONANG 0.5g/Kg) and Tibetan medicine embodiment 1 powder (1.5g/kg of the present invention, 3g/kg, 6g/kg) basic, normal, high dosage group.Anesthetized rat during experiment, then abdominal part cropping, along median line otch, opens abdominal cavity and finds caecum and colonic junction.Blank group rat injects the normal saline containing 10% india ink in colonic lumen in this portion, administration group then injects the medicinal liquid containing 10% india ink, administration volume 2.5mL/kg body weight.5min after administration, opens abdominal cavity immediately, is separated and cuts large intestine, takes out, naturally straightens, and measures the distance of large intestine length and prepared Chinese ink propelling, calculate ink propulsive rate, carbon ink propelling rate=carbon ink advance distance/large intestine total length × 100% with meter ruler.T checks the significance of each group difference, the results are shown in Table 5.
Table 5 Tibetan medicine of the present invention embodiment 1 powder is on the impact (x ± s, n=10) of the large Intestinal pushing of normal rat
Group Animals number dosage (g/kg) large intestine length (cm) carbon ink advance distance (cm) ink propulsive rate (%)
Blank group 10 16.3 ± 1.46.2 ± 2.138.8 ± 15.2
Positive controls 100.515.8 ± 0.38.7 ± 3.354.8 ± 22.6
*
Low dose group 101.516.9 ± 1.29.3 ± 3.1
*59.3 ± 24.7
*
Middle dosage group 10316.4 ± 1.711.1 ± 5.2
*62.4 ± 23.8
*
High dose group 10615.3 ± 1.710.8 ± 2.1
*74.6 ± 23.3
*
Illustrate: compare with model control group:
*p<0.05;
*p<0.0l.
6, Tibetan medicine embodiment 1 powder of the present invention is on mice intestinal volume impact test: animal and grouping with 3, after water 12h is can't help in fasting, respectively organize according to dosage gastric infusion, blank group to equivalent distilled water, administration volume 1mL/20g body weight.The cervical vertebra that broken by animal after administration 2.5h is put to death, and cuts off abdominal cavity, exposes intestinal tube, in pylorus lower end and ileocecus ligation, from pylorus, place cuts intestinal tube, carefully cuts mesentery with little shears downwards along intestinal tube, intestinal tube is cut off, precise intestinal tube weight on electronic balance at ileocecus.T checks the significance of each group difference, the results are shown in Table 6.
Table 6 Tibetan medicine embodiment 1 of the present invention powder is on the impact (x ± s, n=10) of mice intestinal volume
Group Animals number dosage (g/kg) intestinal tube weight (g)
Blank group 10 1.28 ± 0.26
Positive controls 100.51.54 ± 0.47
Low dose group 101.51.48 ± 0.15
Middle dosage group 1031.45 ± 0.27
*
High dose group 1061.56 ± 0.23
*
Illustrate: compare with model control group:
*p<0.05.
7, Tibetan medicine embodiment 1 powder of the present invention is tested mice functions of loosening bowel relieving constipation: animal and grouping with 3, respectively organize according to dosage gastric infusion, blank group to equivalent distilled water, administration volume 1mL/20g body weight.After administration, mice being put into mice box, 1, every box, there is time and the character of soft stool, loose stool, loose stool for the 1st time, represents purging and intestine lubricating action, represent defecating feces excretion with loose stool, represent discharge function with loose stool with soft stool in the clean filter paper of underlay white in record 4h.Soft stool refers to that excrement is shaped, but the soft moisture content that expands is more, and loose stool is shaping or shapeless slightly, scattered paste shape, loose stool scattered paste shape or water liquid.T checks the significance of each group difference, the results are shown in Table 7.
Table 7 Tibetan medicine embodiment 1 of the present invention powder is on the impact (x ± s, n=10) of mice loosening bowel to relieve constipation
Group Animals number dosage (g/kg) soft stool time (min) loose stool time (min) the loose stool time (min)
Blank group 10 134.8 ± 10.3243.0243.0
Positive controls 100.553.5 ± 61.4187.3 ± 91.8243.0
Low dose group 101.552.7 ± 78.9185.2 ± 93.2243.0
Middle dosage group 10336.8 ± 24.6
*128.4 ± 95.7
*205.8 ± 61.4
High dose group 10627.9 ± 24.7
*122.1 ± 96.1
*174.6 ± 83.3
Illustrate: compare with model control group:
*p<0.05;
*p<0.0l.
Result shows: the middle and high dosage of Tibetan medicine embodiment 1 powder of the present invention can make dryness accumulated in the stomach and intestine dehydration Constipation Model mice discharge the time shorten of melena first, the argol that in mice 4h, discharge melena color dot is total is counted and is increased, the number of animals of defecation does not reduce, high dose can make the argol of discharging in DC Constipation Model mice 12h count to increase, loose stool is counted and is increased, and the number of animals of defecation does not reduce; Compare with model control group, its difference all has statistical significance.The middle dosage of Tibetan medicine embodiment 1 powder of the present invention can make to discharge melena color dot sum in normal mouse 4h to be increased, high dose can make normal mouse discharge the time shorten of melena first, discharge melena color dot sum in mice 4h to increase, compare with blank group, its difference also has statistical significance, points out Tibetan medicine embodiment of the present invention 1 pair of dryness accumulated in the stomach and intestine dehydration Constipation Model mice, DC Constipation Model Mus and normal mouse all to have the discharge function promoting animal excretion more significantly.The middle and high dosage of Tibetan medicine embodiment 1 powder of the present invention can promote mouse small intestine prepared Chinese ink advance distance; The basic, normal, high dosage of Tibetan medicine embodiment 1 powder of the present invention can promote rat large intestine prepared Chinese ink advance distance, compare with blank group, its difference all has statistical significance, point out Tibetan medicine embodiment 1 powder of the present invention to have the ahead running promoting intact animal's small intestinal, large intestine, strengthen the function of enterokinesia.The middle and high dosage of Tibetan medicine embodiment 1 powder of the present invention can obviously increase mouse small intestine moisture, mice intestinal tube weight is increased, intestinal volume becomes large, make that soft stool appears in mice, the loose stool time obviously shortens, compare with blank group, its difference all has statistical significance, points out Tibetan medicine embodiment 1 powder of the present invention to have significantly to reduce intestinal to moisture absorption, the functions of loosening bowel relieving constipation that makes stool softening.Above result shows, Tibetan medicine embodiment 1 powder of the present invention has stronger promotion animal defecation, increases enterokinesia and functions of loosening bowel relieving constipation, therefore, has certain value clinically to constipation.
The clinical observation on the therapeutic effect of [test example 2] 1, Tibetan medicine embodiment 1 powder of the present invention treatment constipation:
Physical data: constipation patient is totally 100 examples, is divided into two groups at random by digital method, treatment group 50 example, man 33 example, female 17 example, 53.5 years old mean age; Matched group 50 example, man 35 example, female 15 example, 53 years old mean age.Age of two groups of cases, sex and physical data no significant difference.
Diagnostic criteria: according to " Chinese medical disease Standardization of diagnosis and curative effect ", defecation time extend, two days with the last time, feces dry, hard; Severe one difficulty in defecation, dry as chestnut, few abdominal distention can be accompanied anxious, and refreshing tired weak, stomach poor appetite such as to move back at the disease.
Exclusion standard: get rid of intestinal organic disease.
Therapeutic Method: treatment group: adopt Tibetan medicine embodiment 1 powder of the present invention, oral; A 1.5g, 3 times on the one.Matched group: DAHUANG TONGBIAN JIAONANG, 0.9g/ time, 1 day 1 time, warm water delivery service before sleeping evening.Two groups 15 days is all 1 course for the treatment of to treat, and 1 course for the treatment of terminates to add up therapeutic outcome.
Clinical efficacy evaluation criteria: according to " Chinese medical disease Standardization of diagnosis and curative effect ", cure: defecation 1 time within 2 days, just matter turns profit, and unobstructed during solution, short-term is without recurrence; Take a turn for the better: defecation within 3 days, just matter turns profit, and defecation owes smooth; Do not heal: symptom is without improvement.Healing adds improvement and adds up to total effective rate.
Clinical observation result is in table 8.
Table 8 liang group comparitive study (example, %)
Group number of cases cures the more total effective rate that takes a turn for the better not
Treatment group 503711296.00
*
Matched group 503114590.00
Note: compare with matched group:
*p<0.05.
Untoward reaction: have no adverse reaction in two groups of therapeutic processes; Two groups of courses for the treatment of terminate rear check hepatic and renal function all normal.
Clinical observation result shows: Tibetan medicine embodiment 1 powder treatment constipation determined curative effect of the present invention, clinic is promoted the use of, and has certain application prospect.
2, the clinical observation on the therapeutic effect of Tibetan medicine embodiment 1 powder treatment chronic constipation of the present invention:
Physical data: 80 routine patients, all meets chronic constipation diagnostic criteria in the Roman II diagnostic criteria of constipation and Chinese Medical Association's constipation diagnosis and treatment tentative standard.Wherein man 33 example, female 47 example, 33 ~ 71 years old age, average 50.5 years old, the course of disease 2.1 ~ 11 years, average course of disease 27.12 months.
Therapeutic Method: adopt Tibetan medicine embodiment 1 powder of the present invention, oral; A 1.5g, 3 times on the one.It within 15 days, was 1 course for the treatment of.
Clinical efficacy evaluation criteria: evaluate according to Ministry of Public Health Clinical Researches of New Drugs guideline.(1) effective: to take medicine in latter 3 days and reach following index: 1. defecation frequency recovers normal; 2. feces character transfers to normally; 3. defecation is unobstructed and without difficulty, without sense of discomfort during defecation.(2) effective: in 3 indexs listed in effective, to reach 1 ~ 2 person.(3) invalid: after taking medicine 3 days, These parameters all no significant difference front with treatment.
Therapeutic outcome: this organizes 80 routine patients, after terminating the course for the treatment of, effective 52 examples (65.00%); Effective 24 examples (30.00%); Invalid 4 examples (5.00%).Total effective rate 95.00%.Effective 52 examples of call-on back by phone still effective 46 examples (88.46%) after 60 days; Still effective 17 examples (70.83%) in effective 24 examples.I.e. total effective rate 78.75% after 60 days.
Therapeutic outcome shows: Tibetan medicine embodiment 1 powder treatment constipation determined curative effect of the present invention, clinic is promoted the use of.
In the preparation of conventional oral solid formulation, the preparation of powder be in other conventional oral solid formulation preparation process before process portion, the test example of powder demonstrates has good effect in treatment constipation, also just describes the pill in conventional oral solid formulation, granule, capsule, tablet also have same effect; Namely the above-mentioned embodiment 1-5 enumerated has same effect.Illustrate that Tibetan medicine of the present invention has good effect in treatment constipation.
Claims (5)
1. treat the Tibetan medicine of constipation for one kind, it is characterized in that it is made up of active component or is made up of active component and pharmaceutically acceptable adjuvant, wherein said active component is made up of following bulk drugs: ZANGQIANCAO 20 ~ 60g, Sal Ammoniacus 15 ~ 45g, Tronae 15 ~ 45g, sausage fruit 25 ~ 75g, Herba Oxytropis Kansuensis 15 ~ 45g, Folium Paraquilegiae microphyllae 12.5 ~ 37.5g, Semen Sesami Nigrum 10 ~ 30g, Caulis et Folium Lini 7.5 ~ 22.5g, Semen Ricini 5 ~ 15g.
2. Tibetan medicine according to claim 1, wherein said active component is made up of following bulk drugs: ZANGQIANCAO 32 ~ 48g, Sal Ammoniacus 24 ~ 36g, Tronae 24 ~ 36g, sausage fruit 40 ~ 60g, Herba Oxytropis Kansuensis 24 ~ 36g, Folium Paraquilegiae microphyllae 20 ~ 30g, Semen Sesami Nigrum 16 ~ 24g, Caulis et Folium Lini 12 ~ 18g, Semen Ricini 8 ~ 12g.
3. Tibetan medicine according to claim 2, wherein said active component is made up of the crude drug of following concrete weight: ZANGQIANCAO 40g, Sal Ammoniacus 30g, Tronae 30g, sausage fruit 50g, Herba Oxytropis Kansuensis 30g, Folium Paraquilegiae microphyllae 25g, Semen Sesami Nigrum 20g, Caulis et Folium Lini 15g, Semen Ricini 10g.
4. the Tibetan medicine according to any one of claim 1-3, it is peroral dosage form.
5. Tibetan medicine according to claim 4, it is powder, pill, granule, capsule or tablet.
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