CN104255714B - A kind of tree peony, Chinese herbaceous peony fresh cut-flowers fresh-keeping liquid and preparation method thereof - Google Patents

A kind of tree peony, Chinese herbaceous peony fresh cut-flowers fresh-keeping liquid and preparation method thereof Download PDF

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CN104255714B
CN104255714B CN201410517348.8A CN201410517348A CN104255714B CN 104255714 B CN104255714 B CN 104255714B CN 201410517348 A CN201410517348 A CN 201410517348A CN 104255714 B CN104255714 B CN 104255714B
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CN104255714A (en
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刘献明
杨红兵
杨录臣
余心亮
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Luoyang Normal University
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Abstract

The invention belongs to fresh cut-flowers technical field of preservation of fresh, be specifically related to a kind of tree peony, Chinese herbaceous peony fresh cut-flowers fresh-keeping liquid and preparation method thereof.Fresh-keeping liquid good refreshing effect provided by the invention, effective prolongation tree peony, Chinese herbaceous peony fresh cut-flowers view and admire the time, the tree peony of flower bud phase is made to extend 6-8 days, Chinese herbaceous peony extends 10-11 days, the tree peony of full-bloom stage extends 5-7 days, Chinese herbaceous peony extends 8-10 days, and fresh-keeping liquid of the present invention is safe, nontoxic, pollution-free, and the cutflower preservation phase terminates rear fresh-keeping liquid can recycling.Fresh-keeping liquid fresh-keeping effect of the present invention is excellent, and the scope of application is wider, not only as the fresh-keeping liquid of tree peony, Chinese herbaceous peony fresh cut-flowers, can also be applicable to other fresh cut-flowers fresh-keeping.The product flower arrangement good refreshing effect that method provided by the invention prepares, can increase the popularization of Cut Peony and strengthen the competitiveness in market.Preparation method's technological process provided by the invention is simple, can prepare in a large number, be suitable for suitability for industrialized production.<b />

Description

A kind of tree peony, Chinese herbaceous peony fresh cut-flowers fresh-keeping liquid and preparation method thereof
Technical field
The invention belongs to fresh cut-flowers technical field of preservation of fresh, be specifically related to a kind of tree peony, Chinese herbaceous peony fresh cut-flowers fresh-keeping liquid and preparation method thereof.
Background technology
Tree peony belongs to Paeoniaceae Paeonia machaka, is one of large famous flower of Chinese tradition ten.As a kind of rare flower, tree peony blooms concentrated, and the florescence is of short duration, and large flower and brilliant color, fragrance are strong, and market efficiency is high.Tree peony derived product-Cut Peony, Cut Peony is decayed and is exceedingly fast after departing from parent.The decline of Vitro Plant organ is mainly by the impact of following several factor: (1) lack of moisture; (2) organic nutrient, vitamin and mineral element underfeed; (3) by the injury of ethylene evolution, and tree peony is more responsive to ethene; (4) mechanical damage of cutting pruning; (5) harmful microorganism infects; (6) change of the external environment such as temperature, humidity, illumination, ventilation after in vitro; (7) endogenous hormones is out of control etc.What these factors were facilitated mutually is also restriction mutually.If but above-mentioned condition is improved, then can delay its aging death process in varying degrees.Therefore, correctly grasp the preservation technique of Cut Peony, its ornamental value can be extended, strengthen appreciation effect.
Current tree peony [Chinese herbaceous peony] Research Progress of Keeping Fresh Technology of Cut Flowers mainly contains chemistry and physics two kinds.Chinese invention patent (CN101292649A) discloses tree peony (Chinese herbaceous peony) antistaling agent for cut-flower, is to be made up of sucrose, oxine, citric acid, salicylic acid, chlorine dioxide, silver thiosulfate (STS) and water.This invention can prevent microbial growth, and extend tree peony (Chinese herbaceous peony) the cutflower preservation phase, improve the ornamental quality of cut-flower, and it is few to have consumption, cost is low, feature easy to use; What be not only used in tree peony (Chinese herbaceous peony) cut-flower adopts rear pretreatment fluid, and also can be used for the fresh-keeping of other Cut flowers, flowering stage is longer, and flowering quality is high.The fresh-keeping report of this kind of Cut Peony is more, but antistaling agent prescription nearly all derives using silver nitrate or silver thiosulfate as main component, all there is configuration complex process, not easily preservation in this fresh-keeping liquid composition, cost is higher and poisonous, causes bad impact in varying degrees on human body and environment.Therefore, how to go out a kind of efficient, economic, nontoxic, free of contamination cutting flower fresh-keeping liquid from aspect Integrated Selections such as Antimicrobial preservative, nutritional supplementation, ethene suppressing and prevention dehydrations and just seem particularly necessary.Chinese invention patent (ZL201110350910) has invented a kind of process for preserving tree peony and Chinese herbaceous peony fresh cut-flowers, because tree peony belongs to ethylene-sensitive flowers, control ethylene metabolism and can effectively extend the Cut Peony life-span, temperature is the essential condition controlling ethylene metabolism; Carry out cut-flower at tree peony bud stage, cut part and enter freezer storage after treatment, to postpone flowering time.Through storage after a while, can also be as before fresh after cut-flowers in bottle, brilliance is bursted forth.But the physical fresh-keeping method such as irradiation storage, low temperature storage, because of restrictions such as cost, the scope of application and operating technologies, is difficult to widely use.Therefore find that safe, nontoxic, free of contamination biological way of keeping fresh replaces traditional chemistry, physical fresh-keeping method has great social and economical benefit.
Summary of the invention
The object of the invention is to solve the deficiencies in the prior art, a kind of tree peony, Chinese herbaceous peony fresh cut-flowers fresh-keeping liquid and preparation method thereof are provided, not only safe, nontoxic, and effectively can extend tree peony and Chinese herbaceous peony at the storage time in fresh cut-flowers period, be suitable for extensive popularization.
The present invention for the taked technical scheme that solves the problem is, a kind of tree peony, Chinese herbaceous peony fresh cut-flowers fresh-keeping liquid, described fresh-keeping liquid is the commercially available beneficial bacterium liquid of 0.1-1.2% by volumetric concentration, anhydrous sodium selenate, antioxidant, zinc salt and water are made, and the ratio preparing each component needed for fresh-keeping liquid is: volumetric concentration is the beneficial bacterium liquid of 0.1-1.2%: the selenium weight in anhydrous sodium selenate: antioxidant: the zinc weight in zinc salt: water=1:5 ~ 15:50 ~ 200:10 ~ 40:1, wherein volumetric concentration is the beneficial bacterium liquid of 0.1-1.2%, water is to rise, antioxidant, zinc salt is in mg, anhydrous sodium selenate is in μ g.
The preparation method of described tree peony, Chinese herbaceous peony fresh cut-flowers fresh-keeping liquid, comprises the following steps:
Step one, take each component in the ratio of described each component;
Step 2, anhydrous sodium selenate, antioxidant and zinc salt are put into water, after stirring, obtained mixed liquor, for subsequent use;
Step 3, mixed liquor and the volumetric concentration step 2 prepared are that the beneficial bacterium liquid of 0.1-1.2% mixes by same volume, namely obtain fresh-keeping liquid after stirring.
Described anhydrous sodium selenate is one or more in selenous acid, sodium selenite or potassium selenite.
Described zinc salt is one or more in zinc gluconate, zinc sulphate, zinc nitrate, zinc chloride or zinc acetate.
Described antioxidant is one or more in ascorbic acid, vitamin c or Sodium Benzoate.
The using method of described tree peony, Chinese herbaceous peony fresh cut-flowers fresh-keeping liquid, comprises the following steps:
, prepare some flower arrangement vases, clean after dry, for subsequent use;
, fresh-keeping liquid part the present invention to be prepared is sub-packed in vase, another part is poured in drum, for subsequent use;
, the spray of harvesting is carried out cutting, sterilization and cleaning, afterwards spray is put into the drum that step 3 is equipped with fresh-keeping liquid and floods 3-4s, take out spray afterwards and put into the vase that fresh-keeping liquid is housed;
, the vase being placed with spray is put into the freezer that temperature is 2-4 DEG C.
The present invention's beneficial bacterium liquid used be by Henan Prov. Inst.'s biological study and Rui Teli Bioisystech Co., Ltd of the Henan Province beneficial bacterium stoste " prebiotic king " of producing.
Principle of the present invention is: biological way of keeping fresh comprises that thalline secondary metabolite is fresh-keeping, polysaccharose substance is fresh-keeping, antibacterial peptide is fresh-keeping, biology enzyme is fresh-keeping, direct microbial cells is fresh-keeping, the natural component extract of organism self is fresh-keeping.The mechanism of biological way of keeping fresh delays oxidation or biological preservation material itself has good bacteriostasis.These study mechanism and analysis become the key of exploitation biological preservation liquid.Relative to harmful effects such as fresh chemically liquid environmental pollutions, biological preservation liquid generally can recycling, as soil loosening fertilizer utilization, has very important using value.Wherein microorganism has important effect in biological way of keeping fresh.Beneficial bacterium has no document announcement as the cutting flower fresh-keeping liquid such as tree peony, Chinese herbaceous peony, and it is a kind of active bacteria formulation, and be jointly made up of five large class profitable strains such as photosynthetic bacteria, acetic acid bacteria, saccharomycete, lactic acid bacteria, actinomycetes, be dark brown liquid, pH is 3.8; Its color and luster, taste exactly like the vinegar brewageed; Multiple fields such as beneficial bacterium plant husbandry at home and abroad, aquaculture and environmental protection widely use, safe, effective, pollution-free, without any side effects; And method of operating is easy, with low cost.Beneficial bacterium and other trace elements and antioxidant can produce synergy, collaborative fresh-keeping effect excellence, and are different from fresh chemically liquid, and it has safe, nontoxic, pollution-free, and after cut-flower freshness date terminates, fresh-keeping liquid can recycling.
beneficial effect
One, fresh-keeping liquid good refreshing effect provided by the invention, effective prolongation tree peony, Chinese herbaceous peony fresh cut-flowers view and admire the time, the tree peony of flower bud phase is made to extend 6-8 days, Chinese herbaceous peony extends 10-11 days, the tree peony of full-bloom stage extends 5-7 days, Chinese herbaceous peony extends 8-10 days, and fresh-keeping liquid of the present invention is safe, nontoxic, pollution-free, and the cutflower preservation phase terminates rear fresh-keeping liquid can recycling.
Two, fresh-keeping liquid fresh-keeping effect of the present invention is excellent, and the scope of application is wider, not only as the fresh-keeping liquid of tree peony, Chinese herbaceous peony fresh cut-flowers, can also be applicable to other fresh cut-flowers fresh-keeping.
Three, the product flower arrangement good refreshing effect for preparing of method provided by the invention, can increase the popularization of Cut Peony and strengthen the competitiveness in market.
Four, preparation method's technological process provided by the invention is simple, can prepare in a large number, be suitable for suitability for industrialized production.
Embodiment
Elaborate to embodiments of the invention below, the present embodiment is implemented under premised on technical solution of the present invention, give detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
A kind of tree peony, Chinese herbaceous peony fresh cut-flowers fresh-keeping liquid, described fresh-keeping liquid by volumetric concentration be the commercially available beneficial bacterium liquid of 0.1-1.2%, anhydrous sodium selenate, antioxidant, zinc salt and water makes, and the ratio preparing each component needed for fresh-keeping liquid is: volumetric concentration is the beneficial bacterium liquid of 0.1-1.2%: the selenium weight in anhydrous sodium selenate: antioxidant: the zinc weight in zinc salt: water=1:5 ~ 15:50 ~ 200:10 ~ 40:1, wherein volumetric concentration is the beneficial bacterium liquid of 0.1-1.2%, water to be to rise, antioxidant, zinc salt are in mg, and anhydrous sodium selenate is in μ g.
The preparation method of described tree peony, Chinese herbaceous peony fresh cut-flowers fresh-keeping liquid, comprises the following steps:
Step one, take each component in the ratio of described each component;
Step 2, anhydrous sodium selenate, antioxidant and zinc salt are put into water, after stirring, obtained mixed liquor, for subsequent use;
Step 3, mixed liquor and the volumetric concentration step 2 prepared are that the beneficial bacterium liquid of 0.1-1.2% mixes by same volume, namely obtain fresh-keeping liquid after stirring.
Described anhydrous sodium selenate is one or more in selenous acid, sodium selenite or potassium selenite.
Described zinc salt is one or more in zinc gluconate, zinc sulphate, zinc nitrate, zinc chloride or zinc acetate.
Described antioxidant is one or more in ascorbic acid, vitamin c or Sodium Benzoate.
The using method of described tree peony, Chinese herbaceous peony fresh cut-flowers fresh-keeping liquid, comprises the following steps:
, prepare some flower arrangement vases, clean after dry, for subsequent use;
, fresh-keeping liquid part the present invention to be prepared is sub-packed in vase, another part is poured in drum, for subsequent use;
, the spray of harvesting is carried out cutting, sterilization and cleaning, afterwards spray is put into the drum that step 3 is equipped with fresh-keeping liquid and floods 3-4s, take out spray afterwards and put into the vase that fresh-keeping liquid is housed;
, the vase being placed with spray is put into the freezer that temperature is 2-4 DEG C.
Embodiment 1
A kind of tree peony, Chinese herbaceous peony fresh cut-flowers fresh-keeping liquid, commercially available beneficial bacterium liquid, anhydrous sodium selenate, antioxidant, zinc salt and water that described fresh-keeping liquid is 0.1% by volumetric concentration are made, and the ratio preparing each component needed for fresh-keeping liquid is: volumetric concentration is the beneficial bacterium liquid of 0.1%: the selenium weight in anhydrous sodium selenate: antioxidant: the zinc weight in zinc salt: water=1:5:50:10:1, wherein volumetric concentration is the beneficial bacterium liquid of 0.1-1.2%, water to be to rise, antioxidant, zinc salt are in mg, and anhydrous sodium selenate is in μ g.
The preparation method of described tree peony, Chinese herbaceous peony fresh cut-flowers fresh-keeping liquid, comprises the following steps:
Step one, take each component in the ratio of described each component;
Step 2, anhydrous sodium selenate, antioxidant and zinc salt are put into water, after stirring, obtained mixed liquor, for subsequent use;
Step 3, mixed liquor and volumetric concentration prepared by step 2 be 0.1% beneficial bacterium liquid mix by same volume, i.e. obtained fresh-keeping liquid after stirring.
Described anhydrous sodium selenate is one or more in selenous acid, sodium selenite or potassium selenite.
Described zinc salt is one or more in zinc gluconate, zinc sulphate, zinc nitrate, zinc chloride or zinc acetate.
Described antioxidant is one or more in ascorbic acid, vitamin c or Sodium Benzoate.
The using method of described tree peony, Chinese herbaceous peony fresh cut-flowers fresh-keeping liquid, comprises the following steps:
, prepare some flower arrangement vases, clean after dry, for subsequent use;
, fresh-keeping liquid part the present invention to be prepared is sub-packed in vase, another part is poured in drum, for subsequent use;
, the spray of harvesting is carried out cutting, sterilization and cleaning, afterwards spray is put into the drum that step 3 is equipped with fresh-keeping liquid and floods 3s, take out spray afterwards and put into the vase that fresh-keeping liquid is housed;
, the vase being placed with spray is put into the freezer that temperature is 2 DEG C.
Embodiment 2
A kind of tree peony, Chinese herbaceous peony fresh cut-flowers fresh-keeping liquid, commercially available beneficial bacterium liquid, anhydrous sodium selenate, antioxidant, zinc salt and water that described fresh-keeping liquid is 1.2% by volumetric concentration are made, and the ratio preparing each component needed for fresh-keeping liquid is: volumetric concentration is the beneficial bacterium liquid of 1.2%: the selenium weight in anhydrous sodium selenate: antioxidant: the zinc weight in zinc salt: water=1:15:200:40:1, wherein volumetric concentration be 1.2% beneficial bacterium liquid, water in rise, antioxidant, zinc salt are in mg, and anhydrous sodium selenate is in μ g.
The preparation method of described tree peony, Chinese herbaceous peony fresh cut-flowers fresh-keeping liquid, comprises the following steps:
Step one, take each component in the ratio of described each component;
Step 2, anhydrous sodium selenate, antioxidant and zinc salt are put into water, after stirring, obtained mixed liquor, for subsequent use;
Step 3, mixed liquor and volumetric concentration prepared by step 2 be 1.2% beneficial bacterium liquid mix by same volume, i.e. obtained fresh-keeping liquid after stirring.
Described anhydrous sodium selenate is one or more in selenous acid, sodium selenite or potassium selenite.
Described zinc salt is one or more in zinc gluconate, zinc sulphate, zinc nitrate, zinc chloride or zinc acetate.
Described antioxidant is one or more in ascorbic acid, vitamin c or Sodium Benzoate.
The using method of described tree peony, Chinese herbaceous peony fresh cut-flowers fresh-keeping liquid, comprises the following steps:
, prepare some flower arrangement vases, clean after dry, for subsequent use;
, fresh-keeping liquid part the present invention to be prepared is sub-packed in vase, another part is poured in drum, for subsequent use;
, the spray of harvesting is carried out cutting, sterilization and cleaning, afterwards spray is put into the drum that step 3 is equipped with fresh-keeping liquid and floods 4s, take out spray afterwards and put into the vase that fresh-keeping liquid is housed;
, the vase being placed with spray is put into the freezer that temperature is 4 DEG C.
test 1
One, operation sequence
, prepare some flower arrangement vases, clean after dry, for subsequent use;
, fresh-keeping liquid part the present invention to be prepared is sub-packed in vase, another part is poured in drum, for subsequent use;
, the spray of harvesting is carried out cutting, sterilization and cleaning, afterwards spray is put into the drum that fresh-keeping liquid is housed and floods 3-4s, afterwards take out spray put into the vase that fresh-keeping liquid is housed;
, the vase being placed with spray is put into the freezer that temperature is 3 DEG C.
The present invention's beneficial bacterium liquid used be by Henan Prov. Inst.'s biological study and Rui Teli Bioisystech Co., Ltd of the Henan Province beneficial bacterium stoste " prebiotic king " of producing.
Two, Fresh Keeping Mechanics
Beneficial bacterium is a kind of active bacteria formulation, and be jointly made up of five large class profitable strains such as photosynthetic bacteria, acetic acid bacteria, saccharomycete, lactic acid bacteria, actinomycetes, be dark brown liquid, pH is 3.8; Its color and luster, taste exactly like the vinegar brewageed; Its effect mainly comprises: [1] improves the living environment of in vitro tree peony, Chinese herbaceous peony; Compound beneficial bacterium, in the field planting of spray inside and outside, expansion, occupies advantage gradually, then inhibits growing of all putrefying bacteria; The antibiotics that actinomycetes produce enhances the resistance of in vitro spray; The lactic acid that lactic acid bacteria produces, the acetic acid that acetic acid bacteria produces, makes fresh-keeping liquid pH decline, is all conducive to fresh-keeping; [2] in vitro tree peony, Chinese herbaceous peony provide nutrient; The protein that compound beneficial bacterium produces, amino acid can be used as the nitrogenous source into cut-flower, and its vitamin produced and biochemical enzymes, support the eubolism of spray, and its carbohydrate produced then is made for the respiratory substrate of spray.
Three, result of the test
Through Experimental comparison repeatedly, become blue blue with petal color, lobe is to back, serious dehydration is wilted, lose the mark that terminates as Vase time of ornamental value, fresh-keeping liquid of the present invention at natural temperature 10-25 DEG C, under natural lighting (indoor astigmatism) environment, the Cut Peony life-span that the flower bud phase can be made to adopt cut keeps 6-8 days, and Chinese herbaceous peony can reach 10 days; Tree peony Vase time full-bloom stage being adopted cut keeps about 5.5 days, and Chinese herbaceous peony can reach 8 days.
Test 2
Selenite content is atomic, weighs accurately even if analytical balance is also difficult to ensure.The consumption of vitamin c and zinc gluconate is also very little, during practical application, directly buys finished product sodium selenite sheet, Ascorbyl c plate and zinc gluconate tablets from pharmacy, and more can ensure the accurate of consumption.
One, raw material is taken
Sodium selenite sheet purchased from Shanghai Tiancifu Biological Engineering Co., Ltd., the heavy 0.2mg of every sheet, wherein Se weighs 91.3 μ g; Ascorbyl c plate is purchased from upper Hisense's friendship the Yellow River pharmaceutical Co. Ltd, and every sheet is containing vc100mg; Zinc gluconate tablets purchased from Hainan Pharmaceutical Factory Co., Ltd., every sheet containing zinc gluconate 70mg, the wherein heavy 10mg of Zn; Beneficial bacterium liquid is purchased from Biological Inst., of Henan Provicial Academy of Sciences.
Two, beneficial bacterium Multiple preservative solutions join method: to join 1L
1) getting vitamin C 1, zinc gluconate 2, with being placed in mortar grind into powder, pouring out for subsequent use;
2) get sodium selenite 1, be placed in mortar grind into powder, add 100ml water, at room temperature make it dissolve, obtained sodium selenite solution;
3) in 1000ml measuring tank, add water 500ml, add vitamin C and gluconic acid zinc powder afterwards, draw sodium selenite solution 10ml again to inject wherein, concussion shakes up and makes each components dissolved, last supplementing water to 1000 milliliter scale, adds the beneficial bacterium liquid that 1000ml volumetric concentration is 0.1-1.2% afterwards, concussion shakes up, i.e. obtained fresh-keeping liquid;
4) fresh-keeping liquid part the present invention prepared is sub-packed in vase, and another part is poured in drum, for subsequent use;
Three, the preparation of tree peony, Chinese herbaceous peony fresh cut-flowers and fresh-keeping
1) some vases needed for preparation, clean dry;
2) the fresh-keeping liquid equivalent prepared is sub-packed in each vase;
3) fresh-keeping liquid is poured in drum for subsequent use;
4) take back rapidly indoor prune from field Cai Qielei phase tree peony, Chinese herbaceous peony by cut-flower requirement;
5) the whole strain spray arranged is placed in the drum that fresh-keeping liquid is housed and floods 3-4s, be intended to remove waste heat, cleaning floating dust and sterilization;
6) shake off the moisture on flower after pulling spray out, insert immediately and fill in the vase of fresh-keeping liquid.
Four, the mechanism of action of fresh-keeping liquid
When by mechanical wounding and when being in adverse circumstances because of environment change, the growing amount of free radical surges, and accelerates biological aging thus.There is a kind of glutathione peroxidase GSH-PX then can flutter and obtain free radical, stop the peroxidating of after birth lipid, thus the integrality of protection after birth.And selenium is just the constituent of glutathione peroxidase, play antioxidation by this enzyme.Visible, oxidation resistant essence namely delays senility.Zn in formula, be the required nutrient of plant, VC then strengthens the antioxygenic effect of Se, and bottle floods before inserting in fresh-keeping liquid, ensure that cut-flower not by the invasion and attack of harmful bacteria.
Five, result of the test
At natural temperature 10-25 DEG C, under natural lighting, indoor astigmatism environment, use this fresh-keeping liquid, to turn blue purpling with petal color, back serious dehydration is wilted, and loses the mark that ornamental value terminates as Vase time, the tree peony Vase time that the flower bud phase can be made to adopt cut keeps about 7.5 days, and Chinese herbaceous peony reaches 11 days; Tree peony Vase time full-bloom stage being adopted cut keeps 6.5 days, and Chinese herbaceous peony can reach 10 days.

Claims (5)

1. a tree peony, Chinese herbaceous peony fresh cut-flowers fresh-keeping liquid, it is characterized in that: described fresh-keeping liquid is the commercially available beneficial bacterium liquid of 0.1-1.2% by volumetric concentration, anhydrous sodium selenate, antioxidant, zinc salt and water are made, and the ratio preparing each component needed for fresh-keeping liquid is: volumetric concentration is the beneficial bacterium liquid of 0.1-1.2%: the selenium weight in anhydrous sodium selenate: antioxidant: the zinc weight in zinc salt: water=1:5 ~ 15:50 ~ 200:10 ~ 40:1, wherein volumetric concentration is the beneficial bacterium liquid of 0.1-1.2%, water is to rise, antioxidant, zinc salt is in mg, anhydrous sodium selenate is in μ g, described beneficial bacterium liquid be by Henan Prov. Inst.'s biological study and Rui Teli Bioisystech Co., Ltd of Henan Province produce beneficial bacterium stoste " prebiotic king ".
2. the preparation method of a kind of tree peony according to claim 1, Chinese herbaceous peony fresh cut-flowers fresh-keeping liquid, is characterized in that, comprise the following steps:
Step one, take each component in the ratio of component each described in claim 1;
Step 2, anhydrous sodium selenate, antioxidant and zinc salt are put into water, after stirring, obtained mixed liquor, for subsequent use;
Step 3, mixed liquor and the volumetric concentration step 2 prepared are that the beneficial bacterium liquid of 0.1-1.2% mixes by same volume, namely obtain fresh-keeping liquid after stirring.
3. a kind of fresh cut-flowers fresh-keeping liquid according to claim 1, is characterized in that: described anhydrous sodium selenate is one or more in selenous acid, sodium selenite or potassium selenite.
4. a kind of fresh cut-flowers fresh-keeping liquid according to claim 1, is characterized in that: described zinc salt is one or more in zinc gluconate, zinc sulphate, zinc nitrate, zinc chloride or zinc acetate.
5. a kind of fresh cut-flowers fresh-keeping liquid according to claim 1, is characterized in that: described antioxidant is one or more in ascorbic acid, vitamin c or Sodium Benzoate.
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