Summary of the invention
One object of the present invention is to provide a kind of milk cow forage additive.
Another object of the present invention is to provide the described milk cow forage additive breeding milk cow of application to improve the method for former milk DHA content.By adding additive of the present invention (comprising through wrapping processed micro-algae oil/seawood meal) in the feed of milk cow, utilize the biological transformation function of milk cow, the DHA(DHA by feed) be transformed in cow's milk.Output contains the milk of DHA, and wherein the content of DHA is about 10 ~ 30mg/100g milk.
Another object of the present invention is dairy produce prepared by the former milk that the milk cow providing described use the inventive method to feed produces.
For reaching above-mentioned purpose, on the one hand, the invention provides a kind of milk cow forage additive, described feed contains embedding algal oil/seawood meal, vitamin E and yeast selenium, wherein, embedding algal oil/seawood meal, vitamin E raw material and yeast selenium weight ratio is 100 ~ 500:0.25 ~ 0.5:5 ~ 10.
Wherein further preferably when embedding algal oil, embedding algal oil, vitamin E raw material and yeast selenium weight ratio are 100 ~ 200:0.25 ~ 0.5:5 ~ 10;
When embedding seawood meal, embedding seawood meal, vitamin E raw material and yeast selenium weight ratio are 200 ~ 500:0.25 ~ 0.5:5 ~ 10.
Wherein embed algal oil/seawood meal and should be understood to embedding algal oil or embedding seawood meal, or the mixture of embedding algal oil and embedding seawood meal, the present invention adopts embedding algal oil, embedding seawood meal or its mixture all can realize the object of the invention.
Embedding algal oil/seawood meal wherein can be commercially available prod, or conventionally method preparation.It is current existing technical method.
Can the DHA content preferably embedded further in seawood meal be wherein 10 ~ 15%, the DHA content in algal oil be 30 ~ 40%, the content 40 ~ 60% of vitamin E in vitamin E raw material.
Further, in the further preferred described milk cow forage additive of the present invention, be 20 ~ 80:0.1 ~ 0.3:5 ~ 10 containing DHA, vitamin E and yeast selenium weight ratio; Vitamin E is wherein pure vitamin E.
Wherein said milk cow should be understood to existing any of the right age milk cow, and preferred for this invention be Holstein cow, Simmental, Ayrshire, Guernsey or Jersey.
Those skilled in the art are all clear knows the milk cow age being suitable for giving milk, and the present invention preferably the age is the milk cow of 3 ~ 5 years old.
On the other hand, present invention also offers the method that the described milk cow forage additive breeding milk cow of application improves former milk DHA content, described method comprises with the forage feed milk cow containing feed additive of the present invention, wherein, every day, forage feed amount was guarantee that the intake of DHA is 20 ~ 80 grams/day of heads.
Further, the present invention is preferably while every day, forage feed amount was guarantee that the intake of DHA is 20 ~ 80 grams/day of heads, and the daily intaking amount of yeast selenium is 5 ~ 10 grams/day of heads, and the intake of pure vitamin E is 100 ~ 300mg/ top margin of a page.
Wherein preferably every day, forage feed amount was guarantee that the intake of DHA is 30 ~ 80 grams/day of heads further.
Vitamin E raw material of the present invention refers to the additive containing vitamin, and refers to effective vitamin E component when only there is vitamin E.In vitamin E raw material, the content of vitamin E is 40 ~ 60%.
In order to improve the feeding effect of feed additive of the present invention further, feeding volume every day is equally divided into three times and feeds preferred every day by the present invention.
Namely according to early, middle and late three times feed, each feeding volume be every day feeding volume 1/3.
Wherein 8 hours, further preferred often meal interval.
In described feed, other compositions use prior art normal diet.
And other conditions of breeding milk cow all can conventionally be carried out by common method for breeding.
Concrete is, in the daily ration of ox, add functional additive component (i.e. milk cow forage additive of the present invention), specifically comprise the conventional feed of milk cow+through the DHA algal oil 100 ~ 200g/ top margin of a page+vitamin E (100 ~ 300mg)/top margin of a page+yeast selenium (5 ~ 10) the g/ top margin of a page of embedding treatment.
Adding method is: according to the quantity of ox of feeding by functional feed additive trisection every day, add in breakfast, lunch and dinner, the mode of TMR feed mixing machine or artificial mixing can be used to be mixed with conventional feed (roughage or concentrated feed) by functional feed additive, finally reach mixed uniformly object.Functional feed after mixing carries out routine and feeds.
Need to test the palatability of feed in the process of feeding of functional feed, need 10 ~ 15 days by a definite date raise the phase in advance, because milk cow is unstable to the intake of feed during this, therefore the DHA content in product can be unstable in this stage, can settle out in the intake of the feed through raising after date milk cow in advance, enter feed and stablize the absorption stage, then the DHA content in 10 ~ 15 day time milk can reach stable state.
Wherein about 10 ~ 15 days of the time of raising in advance of functional feed, raise the milk cow of eliminating feed intake deficiency in the phase in advance, replace new ox.Weekly the DHA content in milk is detected, when being greater than 10mg/100g, i.e. desirable milk.From qualified of feeding approximately need 30 days.
Described conventional feed can be any conventional feed in this area, such as roughage (hay, stalk, ensilage), concentrated feed (bran, dregs of beans, soya-bean cake, expanded soybean, cereals), succulent fodder.
Every ox daily feed intake is approximately 6 ~ 8 kilograms, is considered as feed intake deficiency lower than this standard.
Every day divides early by milking system, in, evening gathers milk, is cooled to 2 ~ 4 DEG C of storages, obtains former milk.
Again on the one hand, dairy produce liquid milk prepared by the former milk that present invention also offers the milk cow product using the method for the invention to feed, in described former milk, DHA content is 10 ~ 30mg/100g.
Wherein preferred described dairy produce is liquid milk.
The preparation method of wherein said liquid milk is preferably: filtered by the described former milk gathered, sterilization, after standardization (fat, albumen, non-fat solid), is cooled to less than 30 DEG C cryogenic steriles filling, obtains described liquid milk.
Dairy products be standardized as this area routine operation, such as mainly to be realized by flash distillation, the technique such as concentrated.
Wherein said sterilizing can be pasteurize, or also can carry out conventional UHTS (UHT).
The further preferred described UHTS of the present invention is at 137 ~ 142 DEG C of sterilization 2 ~ 4s.
If need to enrich the kind of product and taste, this liquid milk can be used as base-material, add essence, sweet substance, cereal, nutrient, one or more in Juice and fruit-vegetable fibre, prepare all types of modulation breasts and milk beverage.
The general dairy product obtained through above-mentioned steps is all through sterile filling, and the beverage packaging form that the present invention is preferably common in the market, the plastic bottles such as composite packaging or PET, HDPE, BOPP such as such as Kang Meibao are packed.
In sum, the dairy produce containing DHA that the invention provides a kind of milk cow forage additive and application and obtain.Technical scheme tool of the present invention has the following advantages:
1, without the need to carrying out ectogenic DHA interpolation, proportioning process is eliminated;
2, DHA content stable and with milk strong bonded;
3, do not have external source to add the bad phenomenon such as the fishlike smell brought to occur;
4, the multiple product type of convenient expansion.
Detailed description of the invention
Describe the beneficial effect of implementation process of the present invention and generation below by way of specific embodiment in detail, be intended to help reader to understand essence of the present invention and feature better, not as can the restriction of practical range to this case.
The preparation of embodiment 1 natural DHA original flavor milk
One, feed formula (selection of each raw material meets concerned countries standard):
Feed additive of the present invention
The embedding algal oil 100g/ top margin of a page (wherein containing DHA30 gram)
The vitamin E raw material 250mg/ top margin of a page (wherein containing vitamin e1 00mg)
The yeast selenium 5g/ top margin of a page
Conventional feed: (80% roughage, 20% concentrated feed, roughage is ensilage essence to the 30kg/ top margin of a page
Feed is expanded soybean).
Two, production method
Select ox: holstein cow 3 ~ 5 years old, body weight 600 ± 40KG.
Use feed mixing machine, mix the feed of milk cow, in breakfast, lunch and dinner, often meal is taken in embedding algal oil 33.3g/ and to be eaten head, and vitamin E raw material 83.3mg/ eats head, and yeast selenium 1.67g/ eats head.
Observe the feed intake of milk cow in the stage of raising in advance, after feed intake is stable, start to carry out regular detection, 15 days time intervals to the DHA content in giving milk, equipment is gas chromatograph-mass spectrometer.
1, milk inspection: require fat, albumen, dry, freshness and other indexs all reach raw milk quality standard-required, refrigerates (effectively can suppress the breeding of Microorganism of Milk, ensure the freshness of raw material milk at lay up period) below 4 DEG C.
2, the standardization of milk: fat content >=3.5% requiring batching milk, protein content >=3.0%, real milk solids >=12.3%.
3, homogeneous: first class pressure 200bar, secondary pressure 50bar, homogenizing temperature 70 DEG C.
4, pasteurize, 60 ~ 65 DEG C, 30 minutes.
5, filling.
The preparation of embodiment 2 natural DHA original flavor milk
One, feed formula (selection of each raw material meets concerned countries standard):
Feed additive of the present invention
The embedding algal oil 200g/ top margin of a page (wherein containing DHA80g)
The vitamin E raw material 500mg/ top margin of a page (wherein containing vitamin E 300mg)
The yeast selenium 10g/ top margin of a page
Conventional feed: the 32kg/ top margin of a page (composition is 80% roughage, 20% concentrated feed, and roughage is stalk,
Concentrated feed is expanded soybean).
Two, production method
Select ox: Simmental 3 ~ 5 years old, body weight 550 ± 20KG.
Use feed mixing machine, mix the feed of milk cow, in breakfast, lunch and dinner, often meal is taken in embedding algal oil 66.7g/ and to be eaten head, and vitamin E raw material 166.7mg/ eats head, and yeast selenium 3.33g/ eats head.
Observe the feed intake of milk cow in the stage of raising in advance, after feed intake is stable, start to carry out regular detection, 15 days time intervals to the DHA content in giving milk, equipment is gas chromatograph-mass spectrometer.
1, milk inspection: require fat, albumen, dry, freshness and other indexs all reach raw milk quality standard-required, refrigerates (effectively can suppress the breeding of Microorganism of Milk, ensure the freshness of raw material milk at lay up period) below 4 DEG C.
2, the standardization of milk: fat content >=3.5% requiring batching milk, protein content >=3.0%, real milk solids >=12.3%.
3, homogeneous: first class pressure 200bar, secondary pressure 50bar, homogenizing temperature 70 DEG C.
4, UHT UHTS, 137 ~ 142 DEG C, 4 seconds.
5, filling.
Embodiment 3 is prepared natural DHA and is modulated breast
One, feed formula (selection of each raw material meets concerned countries standard):
Feed additive of the present invention
The embedding algal oil 150g/ top margin of a page (wherein containing DHA45g)
The vitamin E raw material 350mg/ top margin of a page (wherein containing vitamin e1 22.5mg)
The yeast selenium 10g/ top margin of a page
Conventional feed: the 32kg/ top margin of a page (composition 80% roughage, 20% concentrated feed, roughage is hay,
Concentrated feed is expanded soybean).
Select ox: holstein cow 3 ~ 5 years old, body weight 600 ± 40KG.
Use feed mixing machine, mix the feed of milk cow, in breakfast, lunch and dinner, often meal is taken in embedding algal oil 50g/ and to be eaten head, and vitamin E raw material 116.7mg/ eats head, and yeast selenium 3.33g/ eats head.
Observe the feed intake of milk cow in the stage of raising in advance, after feed intake is stable, start to carry out regular detection, 15 days time intervals to the DHA content in giving milk, equipment is gas chromatograph-mass spectrometer.
Milk is checked: require fat, albumen, dry, freshness and other indexs all reach raw milk quality standard-required, refrigerates (effectively can suppress the breeding of Microorganism of Milk, ensure the freshness of raw material milk at lay up period) below 4 DEG C.
Two, formula for a product (in 1000kg product, the selection of each raw material meets concerned countries standard):
Remaining as water.
Two, production method
The production method of the Mongolia flavor liquid milk tea of the present embodiment mainly comprises the following steps:
1, with the milk accounting for batching total amount 1/5th, fruit vegetable powder is dissolved, sweet substance, gellan gum, glycerin monostearate, solution temperature 70 ~ 75 DEG C, dissolution time 15 ~ 20min, speed of agitator 2000r/min.
2, to above-mentioned feed liquid homogeneous: first class pressure 200bar, secondary pressure 50bar.
3, material-compound tank is squeezed into after homogeneous, with remaining milk and water constant volume.
4, essence and B B-complex is added after constant volume.
5, sterilization, obtains the described breast of the modulation containing natural DHA.
The preparation of embodiment 4 natural DHA original flavor milk
One, feed formula (selection of each raw material meets concerned countries standard):
Feed additive of the present invention
The embedding seawood meal 200g/ top margin of a page (wherein containing DHA20g)
The vitamin E raw material 250mg/ top margin of a page (wherein containing vitamin e1 00mg)
The yeast selenium 10g/ top margin of a page
Conventional feed: the 30kg/ top margin of a page (80% roughage, 20% concentrated feed, roughage is ensilage,
Concentrated feed is expanded soybean).
Two, production method
Select ox: beautiful coral ox 3 ~ 5 years old, body weight 580 ± 20KG.
Use feed mixing machine, mix the feed of milk cow, in breakfast, lunch and dinner, often meal is taken in embedding seawood meal 66.7g/ and to be eaten head, and vitamin E 83.3mg/ eats head, and yeast selenium 3.33g/ eats head.
Observe the feed intake of milk cow in the stage of raising in advance, after feed intake is stable, start to carry out regular detection, 15 days time intervals to the DHA content in giving milk, equipment is gas chromatograph-mass spectrometer.
1, milk inspection: require fat, albumen, dry, freshness and other indexs all reach raw milk quality standard-required, refrigerates (effectively can suppress the breeding of Microorganism of Milk, ensure the freshness of raw material milk at lay up period) below 4 DEG C.
2, the standardization of milk: fat content >=3.5% requiring batching milk, protein content >=3.0%, real milk solids >=12.3%.
3, homogeneous: first class pressure 200bar, secondary pressure 50bar, homogenizing temperature 70 DEG C.
4, UHT UHTS, 137 ~ 142 DEG C, 4 seconds.
5, filling.
The preparation of embodiment 5 natural DHA original flavor milk
One, feed formula (selection of each raw material meets concerned countries standard):
Feed additive of the present invention
The embedding seawood meal 250g/ top margin of a page (wherein containing DHA75g)
The vitamin E raw material 500mg/ top margin of a page (wherein containing vitamin E 300mg)
The yeast selenium 5g/ top margin of a page
Conventional feed: (80% roughage, 20% concentrated feed, roughage is 24 to the 30kg/ top margin of a page, concentrated feed
For expanded soybean).
Two, production method
Select ox: Guernsey 3 ~ 5 years old, body weight 500 ± 10KG.
Use feed mixing machine, mix the feed of milk cow, in breakfast, lunch and dinner, often meal is taken in embedding seawood meal 83.3g/ and to be eaten head, and vitamin e1 66.7mg/ eats head, and yeast selenium 1.67g/ eats head.
Observe the feed intake of milk cow in the stage of raising in advance, after feed intake is stable, start to carry out regular detection, 15 days time intervals to the DHA content in giving milk, equipment is gas chromatograph-mass spectrometer.
1, milk inspection: require fat, albumen, dry, freshness and other indexs all reach raw milk quality standard-required, refrigerates (effectively can suppress the breeding of Microorganism of Milk, ensure the freshness of raw material milk at lay up period) below 4 DEG C.
2, the standardization of milk: fat content >=3.5% requiring batching milk, protein content >=3.0%, real milk solids >=12.3%.
3, homogeneous: first class pressure 200bar, secondary pressure 50bar, homogenizing temperature 70 DEG C.
4, UHT UHTS, 137 ~ 142 DEG C, 4 seconds.
5, filling.
The preparation of embodiment 6 natural DHA original flavor milk
One, feed formula (selection of each raw material meets concerned countries standard):
Feed additive of the present invention
The embedding seawood meal 400g/ top margin of a page (wherein DHA content 60g)
The vitamin E 200mg/ top margin of a page (wherein content of vitamin E 100mg)
The yeast selenium 5g/ top margin of a page
Conventional feed: (80% roughage, 20% concentrated feed, roughage is 25 to the 32kg/ top margin of a page, concentrated feed
For expanded soybean).
Two, production method
Select ox: Ayrshire 3 ~ 5 years old, body weight 560 ± 20KG.
Use feed mixing machine, mix the feed of milk cow, in breakfast, lunch and dinner, often meal is taken in embedding seawood meal 133.3g/ and to be eaten head, and vitamin e1 0066.7mg/ eats head, and yeast selenium 3.331.67g/ eats head.
Observe the feed intake of milk cow in the stage of raising in advance, after feed intake is stable, start to carry out regular detection, 15 days time intervals to the DHA content in giving milk, equipment is gas chromatograph-mass spectrometer.
1, milk inspection: require fat, albumen, dry, freshness and other indexs all reach raw milk quality standard-required, refrigerates (effectively can suppress the breeding of Microorganism of Milk, ensure the freshness of raw material milk at lay up period) below 4 DEG C.
2, the standardization of milk: fat content >=3.5% requiring batching milk, protein content >=3.0%, real milk solids >=12.3%.
3, homogeneous: first class pressure 200bar, secondary pressure 50bar, homogenizing temperature 70 DEG C.
4, UHT UHTS, 137 ~ 142 DEG C, 4 seconds.
5, filling.
Specific embodiment the DHA content of producing in former milk see the following form one:
Table one
Embodiment | One | Two | Three | Four | Five | Six |
DHA content | 10.7mg/100g | 30.4mg/100g | 25.2mg/100g | 20mg/100g | 18mg/100g | 17mg/100g |
Present invention also offers following contrast test:
Comparative example 1
This comparative example compares other feed additives of filling a prescription and additive of the present invention contrasts, and wherein feed mode and other compositions of feed are with embodiment 1, the results are shown in Table two.
Table two
Do not carry out compatibility according to formula of the present invention to the use of vitamin E and yeast selenium in comparative example, therefore the oxidation of DHA is comparatively obvious, causes its content lower than embodiment one.
The present invention also compares the milk cow that uses conventional feed to feed with use with the addition of the milk cow of the forage feed of additive of the present invention produce the DHA content of former milk:
In the R&D process of product, cows are divided into groups, one group is control group, and one group is experimental group, and experimental group is fed and added feed additive of the present invention, wherein experimental group one component is with embodiment one, experimental group two is with the feed of embodiment four, and control group is only fed conventional feed, altogether feeds three months, feed every day 100g algae oil or 300g algae powder, compatibility antioxidant, every month is detected the DHA content of giving milk, and experimental result is as following table three:
Table three
? |
One month |
Two months |
Three months |
Control group (conventional feed) |
DHA0mg/100g |
DHA1mg/100g |
DHA0mg/100g |
Experimental group one (embedding algae oil 100g) |
DHA11mg/100g |
DHA15mg/100g |
DHA22mg/100g |
Experimental group two (embedding algae powder 300g) |
DHA10.6mg/100g |
DHA14mg/100g |
DHA24mg/100g |
Test example 1
Present invention also offers following test example, to investigate the repeatability of feed additive of the present invention.To feed 150 holstein cows 3 ~ 5 years old, body weight 600 ± 30kg, feed formula and feeding method with embodiment 4, three months by a definite date, one week raise the phase in advance, monthly give milk to 150 oxen and get comprehensive sample censorship DHA content, data result is as following table four:
Table four
One month |
Two months |
Three months |
DHA12mg/100g |
DHA15mg/100g |
DHA24mg/100g |
This test example illustrates that feed additive provided by the present invention has good repeatability.