CN115251238A - Feed additive composition, application thereof and feed - Google Patents
Feed additive composition, application thereof and feed Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K20/00—Accessory food factors for animal feeding-stuffs
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- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
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Abstract
The invention provides a feed additive composition for improving DHA content in milk, application thereof and feed comprising the feed additive composition. The feed additive composition comprises one or more of grape seed oil, grape seed oil and algae powder, and the DHA content in the milk can be increased by adding the grape seed oil or the optimized combination of the grape seed and the algae powder into the daily ration of the dairy cow.
Description
Technical Field
The invention belongs to the field of feed manufacturing, and particularly relates to a feed additive composition for dairy cow daily ration and a feed containing the same.
Background
Docosahexaenoic acid (DHA) is an omega-3 polyunsaturated fatty acid and has significant effects on brain development, improving heart health, improving vision, and reducing inflammatory response. However, the DHA naturally occurring in humans is only in small amounts and is mainly ingested via dietary sources, such as meat, dairy products or eggs. Therefore, milk products with higher DHA content are desired.
However, the direct addition of DHA to dairy products often has problems of poor absorption and functionality. Therefore, there is a need for a method for effectively increasing the content of DHA that can be efficiently absorbed in a dairy product.
Disclosure of Invention
The invention aims to provide a feed additive composition containing DHA, which strengthens unsaturated fatty acid nutrient contents in daily ration through a feeding end, utilizes metabolic transformation of a cow organism to improve the native DHA content in raw milk and has better absorptivity and functionality.
According to one aspect of the invention, a feed additive composition for increasing the DHA content of milk is provided, and comprises one or more of grape seeds, grape seed oil and algae powder.
Preferably, the algae powder is DHA algae powder with fat content accounting for more than 10% of total fatty acid of the algae powder and DHA content being more than or equal to 13%.
Preferably, the feed additive composition further comprises a functional nutritional package containing one or more of vitamin E and organic selenium. The functional nutritional bag comprises 25-50 parts by weight of vitamin E and 10-75 parts by weight of organic selenium.
Preferably, the feed additive composition is a dairy cow ration additive composition.
Preferably, the feed additive composition comprises 0.4-3.2% grape seed oil, 0.4-3.2% grape seed, 0.4-2% algae meal, based on the total weight of daily ration dry matter.
Preferably, the functional nutritional package is added in an amount of 0.004-0.02% based on the total weight of the daily ration dry matter.
According to another aspect of the present invention, there is provided a feed comprising the feed additive composition.
Preferably, the feed additive composition is added in an amount of 0.4-8% based on the total weight of the feed.
According to a further aspect of the present invention, there is provided the use of the feed additive composition as described above for increasing the DHA content of milk.
According to the feed additive composition, the grape seed oil, the grape seeds and the algae powder are combined, and the unsaturated fatty acid nutrient content of the ration is enhanced at the feeding end, so that the content of raw DHA in the raw milk is increased.
Detailed Description
Technical features, objects and advantages of the present invention will be more clearly understood and appreciated by those skilled in the art. It should be understood that the following detailed description is merely exemplary, and the technical aspects of the present invention are not limited to the specific embodiments listed below.
The invention provides a nutrition regulation method for increasing DHA content in milk by adding a feed additive composition with a specific composition into a dairy cow daily ration formula, wherein the feed additive composition comprises one, two or three of grape seeds, grape seed oil and algae powder.
The grape seeds adopted by the invention are powder formed by crushing and processing the grape seeds, and the fat content accounts for more than 20 percent of the raw material.
The grape seed oil is in the form of microcapsule powder (grape seed oil microcapsule powder) obtained by coating, and has fat content of more than 55%.
The algae powder available in the invention can be DHA algae powder, the fat content of the algae powder accounts for more than 10% of the total fatty acid, and the DHA content is more than or equal to 13%. The algae powder may be in the form of powder obtained by processing algae.
The feed additive composition may comprise 0.4-3.2% of grape seed oil, 0.4-3.2% of grape seed, and 0.4-2% of algae powder, based on the total weight of dry daily ration.
In order to improve the anti-oxidative stress capability of the dairy cows, the feed additive composition can also comprise a functional nutrition bag. Specifically, the functional nutritional packet may be added in an amount of 0.004-0.02% based on the total weight of the daily ration dry matter. Here, the functional nutritional package may include one or both of vitamin E and organic selenium. Preferably, the functional nutritional pack may include 25 to 50 parts by weight of vitamin E and 10 to 75 parts by weight of organic selenium. The organic selenium can be organic selenium of the type commonly used in feed, such as selenium yeast.
The feed additive composition is added into basic ration for feeding dairy cows according to the specific proportion, for example, the feed additive composition is added into ration concentrated feed, and is uniformly mixed with coarse feed after being uniformly stirred, so that the feed additive composition is used for feeding the dairy cows.
In conclusion, the feed additive composition can promote the anabolism of DHA of the dairy cows, reduce the influence on the health and production performance of organisms of the dairy cows and obviously improve the content of DHA in milk.
According to a specific embodiment of the invention, a feed for feeding dairy cows is provided, which comprises the above feed additive composition. Preferably, the feed may be a basal ration for dairy cows. The feed additive composition may be added in an amount of 0.4-8% based on the total weight of the dry matter of the basal ration.
According to a further embodiment of the present invention, there is provided the use of the above feed supplement composition for increasing the DHA content of milk.
According to another embodiment of the present invention, there is provided a method for increasing the DHA content of milk, comprising adding the feed additive composition described above to a dairy cow ration feed. When the feed containing the feed additive composition is fed to a cow, the unsaturated fatty acid nutrient content is strengthened through the feeding end to increase the DHA content in the milk, and the influence on the body health and the production performance of the cow is reduced.
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments.
The methods used in the following examples are conventional methods unless otherwise specified, and the reagents used are commercially available reagents unless otherwise specified.
Examples
Animals and starting materials used in the following examples:
healthy lactating cows with lactation period of 90-120 days are fed to 5 cows in each group, the control group is fed with basal ration of cows, and the test group is fed with the composition of test material and basal ration of cows according to the group.
The daily ration comprises the following components: oat, alfalfa, corn silage, tabletting corn, corn flour, cottonseed, soybean hull, beet chips, soybean meal and concentrate.
Table 1: dairy cow basic daily ration formula
Test herds were fed under the same nutrient feeding conditions for 4 weeks on the above diets, in-bed in-pen, three times per day diet (TMR) (8, 14, 30, 20), drinking water freely, and milking in parallel (8, 00, 14, 00, 20) 3 times per day, mixing morning, evening and night milk samples in proportion (1. The milk fat and milk protein content of the test cattle are monitored daily according to the pasture milking system. And counting the feed amount, the residual feed amount and the cattle heads every day. Collecting TMR samples at multiple points, drying at 105 ℃, measuring the dry matter of the daily ration, and calculating the average dry matter feed intake of the whole group. During the test period, the milk yield of each cow in the morning, in the middle and at night is counted every 10 days, and the milk yield is calculated.
Example 1
A feed additive composition test material consisting of one or more of grape seed oil, grape seeds and DHA algae powder is prepared, and the weight ratio of the three is respectively 0. Adding the test feed into the basic daily ration of the dairy cow, wherein the addition amount of the test feed accounts for 0.4% of the total weight of the dry matter of the daily ration, feeding for 30 days according to the feeding method, and detecting the DHA content in the milk.
The basal ration without the above feed additive composition was used as a control. After feeding for 30 days, the change of DHA content in the milk of each group is compared.
Table 2: effect of feed additive composition on DHA content (mg/100 ml) in milk
As can be seen from Table 2, after feeding for 30 days, the DHA content in milk was higher in the group 1 (0.2% grape seed and 0.2% DHA algal powder) than in the control group (no addition) by 0.1mg/100g, higher in the group 2 (0.2% grape seed oil and 0.2% DHA algal powder) than in the control group by 0.2mg/100g, and lower in the group 3 (0.16% grape seed oil, 0.16% grape seed and 0.08% DHA algal powder) than in the control group by 0.6mg/100g. The addition amounts of grape seed oil, grape seeds and algae powder in the above test 1-3 groups were respectively lower than 0.4-3.2%, 0.4-3.2% and 0.4-2%, so that the DHA concentration in milk was limited (test 1 group and test 2 group) and even decreased (test 3 group).
Example 2:
a feed additive composition test material consisting of one or more of grape seed oil, grape seeds and DHA algae powder is prepared, and the weight ratio of the three is respectively 0. Adding the test feed into the basic ration of the dairy cow, wherein the addition amount of the test feed accounts for 0.8% of the total weight of the dry matter of the ration, feeding for 30 days according to the feeding method, and detecting the DHA content in the milk.
The basal ration without the feed additive is used as a control. After feeding for 30 days, the change of DHA content in the milk of each group is compared.
Table 3: effect of feed supplement composition on DHA content (mg/100 ml) in milk
As can be seen from Table 3, after feeding for 30 days, the DHA content in milk was 1.1mg/100g higher in the test group 4 (0.4% grape seed and 0.4% DHA algal powder) than in the control group (no addition), 1.2mg/100g higher in the test group 5 (0.4% grape seed oil and 0.4% DHA algal powder) than in the control group, and 0.3mg/100g lower in the test group 6 (0.32% grape seed oil, 0.32% grape seed and 0.16% DHA algal powder) than in the control group. The amounts of grape seed oil, grape seeds and algae powder added in the above test 4 and test 5 groups were in the ranges of 0.4-3.2%, 0.4-3.2% and 0.4-2%, respectively, and thus the DHA concentration in milk was increased. The addition amounts of the three components of the grape seed oil, the grape seeds and the DHA algal powder in the test 6 groups are lower than the above ranges, so that the DHA concentration in the milk is reduced.
Example 3:
a feed additive composition test material consisting of one or more of grape seed oil, grape seeds and DHA algae powder was prepared, and the weight ratios of the three were 0. Adding the test feed into the basic ration of the dairy cow, wherein the addition amount of the test feed accounts for 2% of the total weight of the dry matter of the ration, feeding for 30 days according to the feeding method, and detecting the DHA content in the milk.
The basal ration without the feed additive is used as a control. After feeding for 30 days, the change of DHA content in the milk of each group is compared.
Table 4: effect of feed supplement composition on DHA content (mg/100 ml) in milk
As can be seen from table 4, after feeding for 30 days, the DHA content in milk was 1.6mg/100g higher in test 7 (1% grape seed oil and 1% DHA algae powder) than the control group, 2.1mg/100g higher in test 8 (1% grape seed oil and 1% DHA algae powder) than the control group, and 1mg/100g higher in test 9 (0.8% grape seed oil, 0.8% grape seed oil and 0.4% DHA algae powder) than the control group. The three components of the test 7-9 groups are respectively in the ranges of 0.4-3.2%, 0.4-3.2% and 0.4-2%, so that the DHA content in the milk can be obviously improved.
Example 4:
a feed additive composition test material consisting of one or more of grape seed oil, grape seeds and DHA algae powder is prepared, and the weight ratio of the three is respectively 0. Adding the test feed into the basic ration of the dairy cow, wherein the addition amount of the test feed accounts for 4% of the total weight of the dry matter of the ration, feeding for 60 days according to the feeding method, and detecting the DHA content in the milk when feeding for 30 days and 60 days.
The basal ration without the feed additive is used as a control. After feeding for 30 days and 60 days, the change of DHA content in the milk of each group is compared.
Table 5: effect of feed supplement composition on DHA content (mg/100 ml) in milk
As can be seen from table 5, for DHA content in milk, after feeding for 30 days, test 10 (2% grape seed oil and 2% DHA algal flour) was 4.7mg/100g higher than the control group, test 11 (2% grape seed oil and 2% DHA algal flour) was 3.9mg/100g higher than the control group, and test 12 (1.6% grape seed oil, 1.6% grape seed oil and 0.8% DHA algal flour) was 0.7mg/100g higher than the control group.
After feeding for 60 days, the 10 groups are 8.3mg/100g higher than the control group, the 11 groups are 6.2mg/100g higher than the control group, and the 12 groups are 2.4mg/100g higher than the control group.
From the above, the three components of the test groups 10-12 were in the ranges of 0.4-3.2%, and 0.4-2%, respectively, and thus the DHA content in milk could be significantly increased.
Example 5:
a feed additive composition test material consisting of one or more of grape seed oil, grape seeds and DHA algae powder is prepared, and the weight ratio of the three is respectively 0. Adding the test feed into the basic ration of the dairy cow, wherein the addition amount of the test feed accounts for 8% of the total weight of the dry matter of the ration, feeding for 60 days according to the feeding method, and detecting the DHA content in the milk when feeding for 30 days and 60 days.
The basal ration without the feed additive was used as a control. After feeding for 30 days and 60 days, the change of DHA content in the milk of each group is compared.
Table 6: effect of feed additive composition on DHA content (mg/100 ml) in milk
As can be seen from table 6, after feeding for 30 days, the DHA content in milk was 1.4mg/100g higher in test group 13 (grape seed 4% and DHA algal powder 4%), 1.2mg/100g higher in test group 14 (grape seed oil 4% and DHA algal powder 4%) and 4.1mg/100g higher in test group 15 (grape seed oil 3.2%, grape seed 3.2% and DHA algal powder 1.6%) than in the control group.
After feeding for 60 days, the test group 13 is higher than the control group by 1.3mg/100g, the test group 14 is higher than the control group by 1.1mg/100g, and the test group 15 is higher than the control group by 8.6mg/100g.
From the above, the content of the three components in the test 13 group and the test 14 group respectively exceeds the upper limits of 0.4-3.2%, 0.4-3.2% and 0.4-2%, which has an effect of increasing the content of DHA in milk, but the content of DHA in milk is increased by a lower extent compared with the test 15 group.
Comparative example 1:
a feed additive composition test material consisting of one or more of grape seed oil, grape seeds and DHA algae powder was prepared, and the weight ratios of the three were 0. Adding the test feed into the basic ration of the dairy cow, wherein the addition amount of the test feed accounts for 12% of the total weight of the dry matter of the ration, feeding for 60 days according to the feeding method, and detecting the DHA content in the milk when feeding for 30 days and 60 days.
The basal ration without the feed additive is used as a control. After feeding for 30 days and 60 days, the change of DHA content in the milk of each group is compared.
Table 7: effect of feed additive composition on DHA content (mg/100 ml) in milk
As can be seen from Table 6, after feeding for 30 days, the DHA content in milk was 0.5mg/100g lower in the 16 groups (6% grape seed oil and 6% DHA algal powder) than in the control group, 0.7mg/100g lower in the 17 groups (6% grape seed oil and 6% DHA algal powder) than in the control group, and 0.4mg/100g lower in the 18 groups (4.8% grape seed oil, 4.8% grape seed oil and 2.4% DHA algal powder) than in the control group.
After feeding for 60 days, the weight of the test group 16 is 0.6mg/100g lower than that of the control group, the weight of the test group 17 is 0.8mg/100g lower than that of the control group, and the weight of the test group 18 is 0.5mg/100g lower than that of the control group.
As can be seen from the above, the contents of the three components in the groups 16-18 of the tests exceed the ranges of 0.4-3.2%, 0.4-3.2% and 0.4-2%, respectively, which has no effect of increasing the DHA content in the milk, but reduces the DHA content in the milk.
Example 6:
preparing a feed additive composition test material consisting of one or more of grape seed oil, grape seeds and DHA algae powder and a functional nutrition bag (50 parts by weight of vitamin E and 50 parts by weight of organic selenium), wherein the weight ratio is as follows:
trial 19 groups: 2% grape seed oil +2% dha algal flour +0.004% functional nutritional package;
run 20 groups: 2% of grape seeds +2% of DHA algae powder +0.01% of functional nutrition bag;
run 21 group: 1.6% of grape seed oil, 1.6% of grape seeds and 0.8%, DHA algae powder and 0.02% of functional nutrition bag.
Adding the test feed into basic ration of milk cow, feeding for 60 days according to the feeding method, and detecting DHA content in milk at 30-60 days.
Table 8: effect of feed additive composition on DHA content (mg/100 ml) in milk
As can be seen from Table 8, for DHA content in milk, after feeding for 30 days, the content of DHA in the milk was 15.3mg/100g higher in the test 19 group than in the control group, 16.4mg/100g higher in the test 20 group than in the control group, and 9.8mg/100g higher in the test 21 group than in the control group.
After feeding for 60 days, the 19 groups are 17.3mg/100g higher than the control group, the 20 groups are 18.4mg/100g higher than the control group, and the 21 groups are 10.6mg/100g higher than the control group.
As can be seen from table 8, when the feed additive composition contains grape seeds, grape seed oil, DHA algae powder and functional nutritional supplement in the specified ranges, the DHA content in milk can be greatly increased at a relatively low addition ratio.
Example 7:
preparing a feed additive composition test material consisting of one or more of grape seed oil, grape seeds and DHA algae powder and a functional nutrition bag (25 parts by weight of vitamin E and 75 parts by weight of organic selenium), wherein the weight ratio is as follows:
run 22 group: 3.2% grape seed oil +2% dha algal powder +0.004% functional nutritional package;
test 23 groups: 3.2% of grape seeds +2% of DHA algal powder +0.01% of functional nutritional package;
test 24 groups: 3.2% grape seed oil +3.2% grape seeds +1.5% dha algae powder +0.02% functional nutritional package.
Adding the test feed into basic ration of milk cow, feeding for 60 days according to the feeding method, and detecting DHA content in milk at 30-60 days.
The basal ration without the feed additive is used as a control. After feeding for 30 days and 60 days, the change of DHA content in the milk of each group is compared.
Table 9: effect of feed supplement composition on DHA content (mg/100 ml) in milk
As can be seen from Table 9, for DHA content in milk, after feeding for 30 days, the amount of DHA in the milk was 18.7mg/100g higher in the test 22 group than in the control group, 20.2mg/100g higher in the test 23 group than in the control group, and 10.3mg/100g higher in the test 24 group than in the control group.
After feeding for 60 days, the 22 groups are 22.5mg/100g higher than the control group, the 23 groups are 26.9mg/100g higher than the control group, and the 24 groups are 14.1mg/100g higher than the control group.
In the functional nutrition package in the test example, along with the increase of the addition amount of each component, the DHA content shows an ascending trend, and when the addition amount is increased to a certain amount, the DHA content does not increase any more, and the content of the functional nutrition package is influenced by the excessively high or excessively low addition amount.
The above description is only of the preferred embodiments of the invention. It will be appreciated that various modifications, combinations, alterations, and substitutions of the details and features of the invention may be made by those skilled in the art without departing from the spirit and nature of the invention. Such modifications, combinations, alterations, and substitutions are also to be considered as included within the scope of the present invention as claimed.
Claims (10)
1. A feed additive composition for increasing DHA content in milk is characterized by comprising one or more of grape seed, grape seed oil and algae powder.
2. The feed additive composition of claim 1, wherein the algae meal is DHA algae meal with a fat content of 10% or more based on total fatty acids of the algae meal and a DHA content of 13% or more.
3. The feed supplement composition of claim 1, further comprising a functional nutritional package comprising one or more of vitamin E and organic selenium.
4. The feed additive composition of any one of claims 1 to 3, which is a dairy cow ration additive composition.
5. The feed additive composition of claim 4, comprising 0.4-3.2% grape seed oil, 0.4-3.2% grape seed, 0.4-2% algae meal, based on the total weight of daily ration dry matter.
6. The feed additive composition of claim 4, wherein the functional nutritional supplement is added in an amount of 0.004-0.02% based on the total weight of daily ration dry matter.
7. The feed supplement composition of claim 6, wherein the functional nutritional package comprises 25 to 50 parts by weight of vitamin E and 10 to 75 parts by weight of organic selenium.
8. A feed comprising the feed supplement composition of any one of claims 1 to 7.
9. The feed of claim 8, wherein the feed additive composition is added in an amount of 0.4-8% based on the total weight of the feed.
10. Use of a feed additive composition according to any of claims 1-7 for increasing the DHA content of milk.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104206701A (en) * | 2013-05-31 | 2014-12-17 | 内蒙古伊利实业集团股份有限公司 | Cow forage additive, application and obtained DHA-contained dairy product |
| CN107249348A (en) * | 2015-02-02 | 2017-10-13 | 拜内梅尔克公司 | Animal feed composition and preparation method thereof |
| CN114568592A (en) * | 2020-11-30 | 2022-06-03 | 内蒙古伊利实业集团股份有限公司 | Nutritional functional bag for improving content of unsaturated fatty acid components and application and feed thereof |
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2021
- 2021-04-29 CN CN202110473829.3A patent/CN115251238A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104206701A (en) * | 2013-05-31 | 2014-12-17 | 内蒙古伊利实业集团股份有限公司 | Cow forage additive, application and obtained DHA-contained dairy product |
| CN107249348A (en) * | 2015-02-02 | 2017-10-13 | 拜内梅尔克公司 | Animal feed composition and preparation method thereof |
| CN114568592A (en) * | 2020-11-30 | 2022-06-03 | 内蒙古伊利实业集团股份有限公司 | Nutritional functional bag for improving content of unsaturated fatty acid components and application and feed thereof |
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