CN105454673A - Production method for algae DHA extract coated functional milk through coating technology - Google Patents
Production method for algae DHA extract coated functional milk through coating technology Download PDFInfo
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- CN105454673A CN105454673A CN201510928173.4A CN201510928173A CN105454673A CN 105454673 A CN105454673 A CN 105454673A CN 201510928173 A CN201510928173 A CN 201510928173A CN 105454673 A CN105454673 A CN 105454673A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
- A23C9/1528—Fatty acids; Mono- or diglycerides; Petroleum jelly; Paraffine; Phospholipids; Derivatives thereof
Abstract
The invention discloses a production method for algae DHA extract coated functional milk through a coating technology and relates to the technical field of functional milk production methods. Natural algae extract containing DHA is purchased to be subjected to coating treatment; the coated DHA containing algae extract is used for feeding dairy cattle, and the transfer amount of the transferable DHA in blood is measured; a feeding experiment is performed on the dairy cattle directly after evaluation and test of blood transfer, the amount of the coated DHA containing algae extract is added each week, and the DHA content in milk is tested and analyzed each week. It is proved that after the coated DHA algae extract is used for feeding the dairy cattle, the average of the DHA transfer rate in the final milk is 6.2%, and the DHA content in the milk after feeding for four weeks meets the standard. The method is easy to operate, the natural DHA algae is extracted from natural products to be used for feeding the dairy cattle directly, so that DHA is transferred to the milk to the largest extent, and the DHA content of the produced milk is maximized.
Description
Technical field
The present invention relates to and use coating technique bag by the production method of the functional milk of algae DHA extract, belong to the production method technical field of functional milk.
Background technology
Eicosapentaenoic acid (the EPA of Omega-3 aliphatic acid, and DHA (DHA C20:5), C22:6) omega-6 unrighted acid is contrasted, the content of triacylglycerol in blood plasma (TAG) and low density cholesterol can be reduced, angiocardiopathy preventing there is certain effect, also blood cholesterol level can be reduced, there is the various angiocardiopathy of prevention, anti-tumor disease, the effect of prevention learning ability, decrease of memory, in zoopery, above effect manifests too.
EPA property relevant to cyclooxygenase the arachidonic acid (C20:4) of DHA and omega-6 aliphatic acid becomes antagonism, the composition of eicosane acids in body can be impelled to change, confirmed to regulate body physiological.Especially the main composition composition-DHA of retina and brain phosphatide, has important impact to the growth of child brain tissue and optic nerve.
Children absorb 500ml every day and add the milk of vegetable oil and sunflower oil and absorb 7 months continuously, and T-CHOL amount will reduce 7.2%, and neutral lipid reduces 13.2%, LDL-cholesterol level and reduces 9.5%.When adding fish oil in the low-fat milk of degreasing, containing EPA+DHA300mg/100ml, when young people absorbs 6 weeks continuously, neutral lipid will reduce 19%, and HDL-C increases by 19%.
Containing the average 300mg/ milk of EPA and DHA, when being consolidated to 100ml, only picked-up 6 weeks, blood plasma fatty acid content will rise 25-50%, and T-CHOL will reduce 4-11%, and LDL-cholesterol will reduce 6-20%, and neutral lipid will reduce 10-24%.
Reading ability poor 7-9 year is picked in school children the key school in Oxfordshire, Britain area, totally 362 experimental subjects by name, carry out following experiment, every day time of 16 weeks takes algaeDHA, the maize/soybean oil of contrast 600mg, the possibility of promoting sleep is investigated, according to the investigation of sleep habit, treatment therapy does not have too much influence to increase sleep, but the number of times decreased average that the respondent having taken seaweeds DHA replenishers wakes up in sleep midway 7 times, length of one's sleep every day adds 58 minutes.
During abundant picked-up omega-3 aliphatic acid, there is certain effect to angiocardiopathy preventing.Can prevent cardiac arrhythmia, thrombus, reduce the numerical value of neutral fat in blood, delay artery sclerosis symptom, improve the function of blood vessel cell, reduce blood pressure, inflammation-inhibiting reacts.Every day, when absorbing EPA and DHA2-4g, neutral fat can reduce 20-40%.
Within 2002, the food nutrition committee of AMA claims adult human male to absorb 1.6g every day, and it is moderate amount that women absorbs 1.1g leukotrienes every day.Adequate rate for omega-3/omega-6 proposes a lot of sayings, and Canada proposes the suggestion intake of 1:6 first time nineteen ninety, and Japan proposes the ratio of 1:4 afterwards.
Be DHA mostly in the fortified milk that market is sold be 100ml benchmark, Nanyang dairy industry 2.5mg, Soul milk 0.5mg, every day dairy industry 16mg, i.e. average out to 10mg.Especially DHA advises that intake is when being decided to be 10mg, 2-3 year children need absorb 1.5L every day, more than 7 years old children need absorb 2.2L every day, just can reach and advise intake every day.
The safety evaluatio result of the strengthening DHA milk that the ox feeding the feed processing formaldehyde produces and general milk, in the new fresh milk that the strengthening DHA milk that the ox feeding the tuna mixed feed processing formaldehyde in addition produces all is specified on WHO in content of formaldehyde 0.013-0.057ppm benchmark, and no significant difference between these 3 kinds of milk, when tuna oil mixed feed for strengthening DHA interpolation formaldehyde is fed to ox, formaldehyde also can not be transferred in milk, and the formaldehyde security of the DHA fortified milk of the ox of the feed processing formaldehyde of feeding also is the level of general milk.
Pork in Omega3 valency aliphatic acid strengthening Animal foodstuffs, milk, cheese, egg, milk powder, applies in the multiple products such as ham and develops.In fortified milk, the research of omega3 valency sequence aliphatic acid is not very smooth before, is also continuing to study according to recent international trend.Especially from 1993, international joint research group was set up in Korea S, and carried out basis and the raising experiment of nearly 10 times, wherein a part of result is delivered.
Dietary Fatty Acids composition forms with butterfat acid very big-difference, reason is before the aliphatic acid composition of feed is utilized into butter oil, first the aliphatic acid of ruminal microorganisms synthesis, the aliphatic acid etc. synthesized again in the aliphatic acid that breast tissue is synthesized again and body adipose tissue, hydrolyzable and the digestion that adds water of ruminant make the characteristic of the aliphatic acid of picked-up there occurs great changes.Due to the digestion reflection that adds water in cud, absorptivity, in body adipose tissue, deposition waits 3 factors, the amount that feed fat directly changes into butter oil is unstable, the unrighted acid digestion 60-90% left/right rotation that probably adds water in cud becomes saturated fatty acid, 1 day fat regimen amount more than 900-1400g time, digestibility can be caused obviously to decline.When the change of intrafat aliphatic acid in cud of therefore absorbing minimizes, consider fat no matter with what form in cud from microorganism protected factor, at present for the method that aliphatic acid is out of shape continually and steadily, also need to proceed research.
Summary of the invention
For the problems referred to above, the technical problem to be solved in the present invention is to provide
fortunewith coating technique bag by the production method of the functional milk of algae DHA extract.
The present invention uses coating technique bag by the production method detailed process of the functional milk of algae DHA extract to be: step one: buy the natural algae extract containing DHA, then carries out bag to the algae extract of DHA processed;
Step 2: according to the rate of transform in DHA additive inspection blood, by bag by after the algae extract of DHA carry out feeding cow, control group adopts the algae extract of general DHA to feed milk cow, with Gyeonggi-Do Korea B pasture for experimental farm, 5 Dutch Holstein-Friesians are the experiment that experimental group object has carried out 10 days, feed the DHA algal class extract of bag quilt to experimental group ox, the feeding volume of every cow head is 300g every day, measures transferable DHA transfer amount in blood every day;
Step 3: carry out administering transgenic directly to milk cow to after blood transfer evaluation test, the quantity of first week experimental group ox is 39, the amount of the DHA algal class extract of the bag quilt of feeding of every ox is 37.5g every day, the amount of the DHA algal class extract of the bag quilt of feeding of second week every ox is 112.5g every day, the amount of the DHA algal class extract of the bag quilt of feeding of the 3rd week every ox is 187.5g every day, the amount of the DHA algal class extract of the bag quilt of feeding of 4th week every ox is 262.5g every day, all carry out detection to the DHA content in milk weekly to analyze, confirmation feeds bag by DHA algal class extract, in final milk, the mean value of the DHA rate of transform is 6.2%, to feed after surrounding in milk DHA content for meeting standard.
As preferably, DHA mobility self-evaluation measuring method in blood in step 2: after the ox of experimental group is fed 24 hours, take a blood sample at the jugular vein place of ox, when blood sampling time is 10:00 in the morning after feed is fed, for preventing blood clotting, in the test tube of anticoagulant process at 4 DEG C, after within 12 hours, placing, under 1500rpm condition, use DHA transfer amount to analyze after carrying out centrifugation in 15 minutes, blood analysis result area calculates the rate of transform.
Beneficial effect of the present invention: it can overcome the drawback of prior art, and the method is simple to operate, extracts natural DHA algal class Direct-fed milk cow, DHA is transferred in milk to greatest extent from natural goods, and the DHA content of output milk is maximized.
detailed description of the invention:
This detailed description of the invention is by the following technical solutions: step one: buy the natural algae extract containing DHA, then carries out bag to the algae extract of DHA processed.
Step 2: according to the rate of transform in DHA additive inspection blood, by bag by after the algae extract of DHA carry out feeding cow, control group adopts the algae extract of general DHA to feed milk cow, with Gyeonggi-Do Korea B pasture for experimental farm, 5 Dutch Holstein-Friesians are the experiment that experimental group object has carried out 10 days, feed the DHA algal class extract of bag quilt to experimental group ox, the feeding volume of every cow head is 300g every day, measures transferable DHA transfer amount in blood every day.
With reference to table 1: according to DHA transfer amount in the DHA algal class extract scale of feeding evaluating blood of bag quilt
| [0001] Distinguish | [0002] 0 day | [0003] 1 day | [0004] 7 days | [0005] 10 days |
| [0006] Control group | [0007] 2.8 | [0008] 2.9 | [0009] 4.1 | [0010] 2.8 |
| [0011] Experimental group 1 | [0012] 2.1 | [0013] 13.57 | [0014] 35.06 | [0015] 59.37 |
| [0016] Experimental group 2 | [0017] 2.5 | [0018] 28.18 | [0019] 72.61 | [0020] 113.96 |
| [0021] Experimental group 3 | [0022] 2.9 | [0023] 16.87 | [0024] 39.54 | [0025] 103.54 |
| [0026] Experimental group 4 | [0027] 3.1 | [0028] 28.54 | [0029] 41.51 | [0030] 102.54 |
| [0031] Experimental group 5 | [0032] 1.8 | [0033] 25.12 | [0034] 29.54 | [0035] 87.52 |
Than the DHA transfer amount of control group, experimental result display experimental group 5 confirms that result is obvious, and along with the increase gradually of number of days, confirm that result is more obvious, when this experiment has been unequivocally established and has fed the DHA algal class extract of bag quilt, the transfer of DHA can be carried out in the blood of ox, DHA mobility self-evaluation measuring method in bovine blood: after the ox of experimental group is fed 24 hours, take a blood sample at the jugular vein place of ox, when blood sampling time is 10:00 in the morning after feed is fed, for preventing blood clotting, in the test tube of anticoagulant process at 4 DEG C, after within 12 hours, placing, under 1500rpm condition, DHA transfer amount is used to analyze after carrying out centrifugation in 15 minutes, blood analysis result area calculates the rate of transform.
Step 3: carry out administering transgenic directly to milk cow to after blood transfer evaluation test, the quantity of first week experimental group ox is 39, the amount of the DHA algal class extract of the bag quilt of feeding of every ox is 37.5g every day, the amount of the DHA algal class extract of the bag quilt of feeding of second week every ox is 112.5g every day, the amount of the DHA algal class extract of the bag quilt of feeding of the 3rd week every ox is 187.5g every day, the amount of the DHA algal class extract of the bag quilt of feeding of 4th week every ox is 262.5g every day, all carry out detection to the DHA content in milk weekly to analyze, confirmation feeds bag by DHA algal class extract, in final milk, the mean value of the DHA rate of transform is 6.2%, to feed after surrounding in milk DHA content for meeting standard.
With reference to table 2: evaluated the rate of transform according to bag by the scale of feeding of DHA algal class extract
| [0036] Distinguish | [0037] Head number | [0038] Scale of feeding (kg/ days) | [0039] Every scale of feeding | [0040] DHA content in milk |
| [0041] 1 week | [0042] 39 | [0043] 1.125 | [0044] 37.5 | [0045] 2mg/100ml |
| [0046] 2 weeks | [0047] 39 | [0048] 3.375 | [0049] 112.5 | [0050] 6mg/100ml |
| [0051] 3 weeks | [0052] 39 | [0053] 7.3125 | [0054] 187.5 | [0055] 10mg/100ml |
| [0056] 4 weeks | [0057] 39 | [0058] 10.2375 | [0059] 262.5 | [0060] 14mg/100ml |
The rate of transform is evaluated according to bag of feeding by the amount of DHA algal class extract with reference to table 3
| [0061] | [0062] 1 week | [0063] 2 weeks | [0064] 3 weeks | [0065] 4 weeks |
| [0066] Bag is by DHA scale of feeding (g/d) | [0067] 37.5 | [0068] 112.5 | [0069] 187.5 | [0070] 262.5 |
| [0071] DHA content (mg/100ml milk) | [0072] 0.52 | [0073] 1.45 | [0074] 3.36 | [0075] 9.29 |
| [0076] The DHA rate of transform (%)-breast amount 30kg benchmark | [0077] 2.40% | [0078] 2.20% | [0079] 3.10% | [0080] 6.20% |
By table 2 and table 3 confirm to feed novel bag by DHA algal class extract time, in the milk that 39 cow heads are extruded, DHA rate of transform mean value is 6.2%, DHA content is 9.29mg/100ml, current adult advises that intake is 10mg/100ml, two are carried out contrasting is same level, and the milk that bag is produced by the milk cow of DHA algal class extract so judgement is fed is development functionality milk.
More than show and describe general principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and description just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.Application claims protection domain is by appending claims and equivalence thereof.
Claims (2)
1. use coating technique bag by the production method of the functional milk of algae DHA extract, it is characterized in that: step one: buy the natural algae extract containing DHA, then bag is carried out to the algae extract of DHA and be processed;
Step 2: according to the rate of transform in DHA additive inspection blood, by bag by after the algae extract of DHA carry out feeding cow, control group adopts the algae extract of general DHA to feed milk cow, with Gyeonggi-Do Korea B pasture for experimental farm, 5 Dutch Holstein-Friesians are the experiment that experimental group object has carried out 10 days, feed the DHA algal class extract of bag quilt to experimental group ox, the feeding volume of every cow head is 300g every day, measures transferable DHA transfer amount in blood every day;
Step 3: carry out administering transgenic directly to milk cow to after blood transfer evaluation test, the quantity of first week experimental group ox is 39, the amount of the DHA algal class extract of the bag quilt of feeding of every ox is 37.5g every day, the amount of the DHA algal class extract of the bag quilt of feeding of second week every ox is 112.5g every day, the amount of the DHA algal class extract of the bag quilt of feeding of the 3rd week every ox is 187.5g every day, the amount of the DHA algal class extract of the bag quilt of feeding of 4th week every ox is 262.5g every day, all carry out detection to the DHA content in milk weekly to analyze, confirmation feeds bag by DHA algal class extract, in final milk, the mean value of the DHA rate of transform is 6.2%, to feed after surrounding in milk DHA content for meeting standard.
2. according to utilization coating technique bag according to claim 1 by the production method of the functional milk of algae DHA extract, it is characterized in that: DHA mobility self-evaluation measuring method in blood in described step 2: after the ox of experimental group is fed 24 hours, take a blood sample at the jugular vein place of ox, when blood sampling time is 10:00 in the morning after feed is fed, for preventing blood clotting, in the test tube of anticoagulant process at 4 DEG C, after within 12 hours, placing, under 1500rpm condition, DHA transfer amount is used to analyze after carrying out centrifugation in 15 minutes, blood analysis result area calculates the rate of transform.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105941948A (en) * | 2016-07-15 | 2016-09-21 | 希杰（沈阳）饲料有限公司 | Method for feeding pigs by pig feed produced through DHA alga |
| CN113115866A (en) * | 2019-12-31 | 2021-07-16 | 内蒙古优然牧业有限责任公司 | Coated algae powder for improving DHA content in ruminant milk and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102160604A (en) * | 2011-03-01 | 2011-08-24 | 厦门汇盛生物有限公司 | Schizochytrium aggregatum powder additive and application thereof to seedling cultivation, aquiculture and culture of poultry and livestock |
| CN104206701A (en) * | 2013-05-31 | 2014-12-17 | 内蒙古伊利实业集团股份有限公司 | Cow forage additive, application and obtained DHA-contained dairy product |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102160604A (en) * | 2011-03-01 | 2011-08-24 | 厦门汇盛生物有限公司 | Schizochytrium aggregatum powder additive and application thereof to seedling cultivation, aquiculture and culture of poultry and livestock |
| CN104206701A (en) * | 2013-05-31 | 2014-12-17 | 内蒙古伊利实业集团股份有限公司 | Cow forage additive, application and obtained DHA-contained dairy product |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105941948A (en) * | 2016-07-15 | 2016-09-21 | 希杰（沈阳）饲料有限公司 | Method for feeding pigs by pig feed produced through DHA alga |
| CN113115866A (en) * | 2019-12-31 | 2021-07-16 | 内蒙古优然牧业有限责任公司 | Coated algae powder for improving DHA content in ruminant milk and preparation method and application thereof |
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