CN104204195B - 以记忆t细胞为主要成分的淋巴细胞群的制造方法 - Google Patents
以记忆t细胞为主要成分的淋巴细胞群的制造方法 Download PDFInfo
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- A—HUMAN NECESSITIES
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Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2012-088926 | 2012-04-10 | ||
JP2012088926A JP6142142B2 (ja) | 2012-04-10 | 2012-04-10 | メモリーt細胞を主成分とするリンパ球細胞群の製造方法 |
PCT/JP2013/002405 WO2013153800A1 (ja) | 2012-04-10 | 2013-04-09 | メモリーt細胞を主成分とするリンパ球細胞群の製造方法 |
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CN104204195A CN104204195A (zh) | 2014-12-10 |
CN104204195B true CN104204195B (zh) | 2017-07-18 |
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CN201380018924.8A Active CN104204195B (zh) | 2012-04-10 | 2013-04-09 | 以记忆t细胞为主要成分的淋巴细胞群的制造方法 |
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JP (1) | JP6142142B2 (ja) |
KR (1) | KR101521484B1 (ja) |
CN (1) | CN104204195B (ja) |
HK (1) | HK1205181A1 (ja) |
WO (1) | WO2013153800A1 (ja) |
Families Citing this family (8)
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JP6424447B2 (ja) * | 2014-03-28 | 2018-11-21 | 東洋製罐グループホールディングス株式会社 | 細胞培養方法、及び細胞培養システム |
CN106796237B (zh) * | 2014-07-22 | 2018-12-04 | 株式会社癌症免疫研究所 | 末梢循环癌细胞的检测装置 |
US10731122B2 (en) | 2015-01-30 | 2020-08-04 | Toyo Seikan Group Holdings, Ltd. | Cell culture method and cell culture apparatus |
CN107384859A (zh) * | 2017-09-05 | 2017-11-24 | 四川新生命干细胞科技股份有限公司 | 一种高纯度nk细胞的分离培养方法 |
AU2018368786A1 (en) * | 2017-11-17 | 2020-06-18 | Iovance Biotherapeutics, Inc. | TIL expansion from fine needle aspirates and small biopsies |
WO2020032782A1 (ko) * | 2018-08-10 | 2020-02-13 | 주식회사 유틸렉스 | 암항원 특이적 cd8+ t 세포의 제조 및 동결보존 방법 |
CN109628396B (zh) * | 2019-01-24 | 2021-03-26 | 清华大学 | 记忆性淋巴细胞群在肝癌治疗中的应用 |
CN113005081B (zh) * | 2019-12-20 | 2023-12-19 | 苏州依科赛生物科技股份有限公司 | 一种扩增干细胞样记忆性t细胞的培养方法 |
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---|---|---|---|---|
CN1363681A (zh) * | 2000-12-04 | 2002-08-14 | 胡曼泰克有限公司 | 源于脐带血的活化淋巴细胞、含有所述淋巴细胞作为主要成分的制剂和制备所述制剂的方法与成套器具 |
CN1230524C (zh) * | 2000-12-04 | 2005-12-07 | 株式会社淋巴技术 | 细胞保存液和使用该保存液的细胞保存方法 |
EP1066833B1 (en) * | 1999-07-08 | 2008-10-01 | Lymphotec Inc. | Use of cryopreserved activated lymphocytes as a medicament |
JP4389039B2 (ja) * | 1998-04-09 | 2009-12-24 | 株式会社リンフォテック | アロジェニックな活性化cd4陽性細胞を主成分とする医薬組成物、およびその製造方法、ならびに該医薬組成物調製用キット |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP4385158B2 (ja) * | 2000-12-04 | 2009-12-16 | 株式会社リンフォテック | 細胞の保存液および該保存液を用いた細胞の保存方法 |
ES2589678T3 (es) * | 2010-09-08 | 2016-11-15 | Yeda Research And Development Co. Ltd. | Uso de linfocitos T de memoria central anti-terceros para el tratamiento anti-leucemia/linfoma |
-
2012
- 2012-04-10 JP JP2012088926A patent/JP6142142B2/ja active Active
-
2013
- 2013-04-09 CN CN201380018924.8A patent/CN104204195B/zh active Active
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Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP4389039B2 (ja) * | 1998-04-09 | 2009-12-24 | 株式会社リンフォテック | アロジェニックな活性化cd4陽性細胞を主成分とする医薬組成物、およびその製造方法、ならびに該医薬組成物調製用キット |
EP1066833B1 (en) * | 1999-07-08 | 2008-10-01 | Lymphotec Inc. | Use of cryopreserved activated lymphocytes as a medicament |
CN1363681A (zh) * | 2000-12-04 | 2002-08-14 | 胡曼泰克有限公司 | 源于脐带血的活化淋巴细胞、含有所述淋巴细胞作为主要成分的制剂和制备所述制剂的方法与成套器具 |
CN1230524C (zh) * | 2000-12-04 | 2005-12-07 | 株式会社淋巴技术 | 细胞保存液和使用该保存液的细胞保存方法 |
Non-Patent Citations (2)
Title |
---|
A feasible method for expansion of peripheral blood lymphocytes by culture with immobilized anti-cd3 monoclonal antibody and interleukin-2 use in a doptive immunotherapy of cancer patients;T Sekine 等;《BIOMED PHARMACOTHER》;19930331;第47卷(第2-3期);73-78 * |
Ex vivo expansion of umbilical cord blood for transplantation;S.S.Tung 等;《Best Practice & Research Clinical Haematology》;20100630;第23卷(第2期);245-257 * |
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JP2013215141A (ja) | 2013-10-24 |
CN104204195A (zh) | 2014-12-10 |
HK1205181A1 (en) | 2015-12-11 |
WO2013153800A1 (ja) | 2013-10-17 |
KR101521484B1 (ko) | 2015-06-05 |
KR20140145585A (ko) | 2014-12-23 |
JP6142142B2 (ja) | 2017-06-07 |
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