CN104203274A - 轮状病毒亚单位疫苗及其制备和使用的方法 - Google Patents
轮状病毒亚单位疫苗及其制备和使用的方法 Download PDFInfo
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- Peptides Or Proteins (AREA)
Abstract
Description
SEQ ID# | # | 说明 |
2 | 650 | GST正向引物,具有NdeI位点 |
3 | 651 | GST反向引物,具有BamHI位点 |
4 | 644 | AMPR基因缺失正向引物 |
5 | 645 | AMPR基因缺失反向引物 |
6 | 660 | PCR验证正向引物. |
7 | 661 | PCR验证反向引物. |
8 | 652 | NSP4正向引物,具有BamHI位点 |
9 | 653 | NSP4反向引物,具有HindIII位点 |
10 | 654 | VP4正向引物,具有BamHI位点 |
11 | 655 | VP4反向引物,具有HindIII位点 |
12 | 656 | VP6正向引物,具有BamHI位点 |
13 | 657 | VP6反向引物,具有HindIII位点 |
73 | 760 | KSN760–反向引物VP4 |
74 | 761 | KSN761–正向引物VP4 |
75 | 762 | KSN762-反向引物VP4 |
76 | 763 | KSN763-正向引物VP4 |
77 | 772 | 将His标记插入pNPL1的引物1 |
78 | 773 | 将His标记插入pNPL1的引物2 |
79 | 774 | 用于pNPL3的Rota C NSP4正向引物 |
80 | 775 | 用于pNPL3或pNPL1的Rota C NSP4反向引物 |
81 | 776 | 用于pNPL3的Rota C VP4正向引物 |
82 | 777 | 用于pNPL3或pNPL1的Rota C VP4反向引物 |
83 | 778 | 用于pNPL3的Rota C VP6正向引物 |
84 | 779 | 用于pNPL3或pNPL1的Rota C VP6反向引物 |
85 | 780 | 用于pNPL1的Rota C NSP4正向引物 |
86 | 781 | 用于pNPL1的Rota C VP4正向引物 |
87 | 782 | 用于pNPL1的Rota C VP6正向吲物 |
88 | 783 | 用于pNPL3的Rota C VP7反向引物 |
89 | 784 | 用于pNPL3或pNPL1的Rota C VP7反向引物 |
CON | VACC | P值 | |
因腹泻引起的死亡率,n | 37 | 23 | |
因腹泻引起的死亡率,% | 1.72 | 1.02 | .19 |
Fallouts,% | .69 | .92 | .53 |
CON | VACC | SEM | P值 | |
第1天 | .56 | .41 | .06 | .05 |
第2天 | .87 | .84 | .07 | .77 |
第3天 | 1.01 | 1.00 | .08 | .98 |
第4天 | .51 | .40 | .10 | .41 |
第5天 | .29 | .24 | .04 | .37 |
CON | VACC | SEM | P值 | |
第1天 | .30 | .32 | .10 | .84 |
第2天 | .61 | .40 | .12 | .15 |
第3天 | .80 | .76 | .13 | .79 |
第4天 | .25 | .15 | .09 | .32 |
第5天 | .02 | .00 | .02 | .31 |
标记 | 体积 |
BamH I和Hind III切割的pNPL2(~50ng/ul) | 3μl |
NSP4PCR产物(~50ng/ul) | 0.5μl |
VP4PCR产物(~50ng/ul) | 1μl |
VP6PCR产物(~50ng/ul) | 1.5μl |
5X融合HD克隆混合物(Cat#639646,Clontech) | 2μl |
不含核酸酶的水 | 2μl |
Claims (15)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201261598624P | 2012-02-14 | 2012-02-14 | |
US61/598,624 | 2012-02-14 | ||
PCT/US2013/026179 WO2013123219A1 (en) | 2012-02-14 | 2013-02-14 | Rotavirus subunit vaccines and methods of making and use thereof |
Publications (2)
Publication Number | Publication Date |
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CN104203274A true CN104203274A (zh) | 2014-12-10 |
CN104203274B CN104203274B (zh) | 2017-09-08 |
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Application Number | Title | Priority Date | Filing Date |
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CN201380015512.9A Active CN104203274B (zh) | 2012-02-14 | 2013-02-14 | 轮状病毒亚单位疫苗及其制备和使用的方法 |
Country Status (22)
Country | Link |
---|---|
US (1) | US9446117B2 (zh) |
EP (1) | EP2814508B1 (zh) |
JP (1) | JP6149240B2 (zh) |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108864260A (zh) * | 2018-07-17 | 2018-11-23 | 昆明学院 | 非结构蛋白nsp4、重组杆状病毒液及制备、粘膜佐剂 |
CN114875047A (zh) * | 2022-05-27 | 2022-08-09 | 江苏三仪生物工程有限公司 | 一种优化的猪轮状病毒外衣壳蛋白vp4的重组表达及应用 |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10004797B2 (en) | 2010-10-27 | 2018-06-26 | Harrisvaccines, Inc. | Method of rapidly producing improved vaccines for animals |
RU2649132C1 (ru) * | 2017-08-03 | 2018-03-29 | Федеральное государственное унитарное предприятие "Государственный научно-исследовательский институт особо чистых биопрепаратов" Федерального медико-биологического агентства | Способ получения активного начала вакцины против ротавирусной инфекции белка FliCVP6VP8 |
CN109306007B (zh) * | 2018-09-26 | 2021-10-08 | 华中农业大学 | 抗猪繁殖与呼吸综合征病毒nsp4蛋白基因工程抗体及应用 |
US20220160864A1 (en) * | 2020-10-05 | 2022-05-26 | Boehringer Ingelheim Animal Health USA Inc. | Fusion protein comprising circoviridae capsid protein, and chimeric virus-like particles composed thereof |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6673355B1 (en) * | 1995-06-14 | 2004-01-06 | Baylor College Of Medicine | Rotavirus enterotoxin NSP4 and methods of using same |
Family Cites Families (36)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2909462A (en) | 1955-12-08 | 1959-10-20 | Bristol Myers Co | Acrylic acid polymer laxative compositions |
US4708871A (en) | 1983-03-08 | 1987-11-24 | Commonwealth Serum Laboratories Commission | Antigenically active amino acid sequences |
EP0173552B1 (en) | 1984-08-24 | 1991-10-09 | The Upjohn Company | Recombinant dna compounds and the expression of polypeptides such as tpa |
US5186933A (en) | 1986-12-30 | 1993-02-16 | Baylor College Of Medicine | Synthesis and immunogenicity of rotavirus genes using a baculovirus expression system |
US5147639A (en) * | 1990-06-19 | 1992-09-15 | Ambico, Inc. | Type-c rotavirus cultures and uses therefor |
US5298244A (en) | 1990-10-25 | 1994-03-29 | University Of Saskatchewan | Assembled viral particles and their use in a vaccine to rotaviral disease |
BE1006085A3 (fr) | 1992-07-31 | 1994-05-10 | Univ Bruxelles | Vecteur de clonage. |
EP0826063A1 (en) | 1995-04-25 | 1998-03-04 | Vical Incorporated | Single-vial formulations of dna/lipid complexes |
ATE248601T1 (de) * | 1995-06-14 | 2003-09-15 | Baylor College Medicine | Impfstoff gegen rotavirus infektionen enthaltend peptide des viralenterotoxines nsp4 |
US5846946A (en) | 1996-06-14 | 1998-12-08 | Pasteur Merieux Serums Et Vaccins | Compositions and methods for administering Borrelia DNA |
FR2751224B1 (fr) | 1996-07-19 | 1998-11-20 | Rhone Merieux | Formule de vaccin polynucleotidique contre les pathologies respiratoires et de reproduction des porcs |
FR2751226B1 (fr) | 1996-07-19 | 1998-11-27 | Rhone Merieux | Formule de vaccin polynucleotidique contre les pathologies du cheval |
FR2751228B1 (fr) | 1996-07-19 | 1998-11-20 | Rhone Merieux | Vaccin polynucleotidique bovin pour voie intradermique |
FR2751227B1 (fr) | 1996-07-19 | 1998-11-27 | Rhone Merieux | Formule de vaccin polynucleotidique contre les pathologies canines, notamment les pathologies respiratoires et digestives |
FR2751225B1 (fr) | 1996-07-19 | 1998-11-27 | Rhone Merieux | Formule de vaccin polynucleotidique aviaire |
FR2751229B1 (fr) | 1996-07-19 | 1998-11-27 | Rhone Merieux | Formule de vaccin polynucleotidique notamment contre la pathologie respiratoire des bovins |
EP1537877A3 (en) | 1996-10-11 | 2005-08-03 | The Regents Of The University Of California | Immunostimulatory polynucleotide/immunomodulatory molecule conjugates |
BR9810369A (pt) | 1997-06-30 | 2000-09-05 | Rhone Poulenc Rorer Sa | Processo de transferência de ácido nucleico para o interior de um ou mais músculos estriados, in vivo, composição, ácido mucleico e campo elétrico, e, produto de combinação |
US7183097B1 (en) | 1998-05-07 | 2007-02-27 | Universite Libre De Bruxelles | Cytotoxin-based biological containment |
FR2778858B1 (fr) | 1998-05-20 | 2000-06-16 | Oreal | Emulsion e/h/e stable et son utilisation comme composition cosmetique et/ou dermatologique |
US6716431B1 (en) | 1998-05-29 | 2004-04-06 | Wyeth Holdings Corporation | Differential cytotoxicity of alternative forms of rotavirus nonstructural protein 4 |
US6187319B1 (en) | 1998-06-01 | 2001-02-13 | University Of Massachusetts | Cross-protective rotavirus vaccine |
US6589529B1 (en) * | 1998-10-30 | 2003-07-08 | Children's Hospital Medical Center | Rotavirus subunit vaccine |
FR2801607B1 (fr) | 1999-11-26 | 2001-12-28 | Merial Sas | Pneumovirus du canard et vaccins correspondants |
US6852705B2 (en) | 2000-01-21 | 2005-02-08 | Merial | DNA vaccines for farm animals, in particular bovines and porcines |
US6867353B2 (en) | 2000-02-12 | 2005-03-15 | Exploregen Inc. | Production method of recombinant rotavirus structural proteins and vaccine composition |
CA2427809A1 (en) * | 2000-11-03 | 2002-05-10 | Baylor College Of Medicine | Rotavirus enterotoxin nsp4 and methods of using same |
WO2004056390A1 (en) | 2002-12-19 | 2004-07-08 | Akzo Nobel Patent Department | Trivalent vaccine with maternal antibody transfer via the milk |
US7371395B2 (en) | 2003-07-24 | 2008-05-13 | Merial Limited | Vaccine formulations |
WO2006007555A2 (en) * | 2004-07-01 | 2006-01-19 | Children's Medical Center Corporation ¨ | Rotavirus antigens |
US7691368B2 (en) * | 2005-04-15 | 2010-04-06 | Merial Limited | Vaccine formulations |
BRPI0504441A (pt) | 2005-10-20 | 2007-06-26 | Fundacao Oswaldo Cruz | vetores de expressão plasmidial contendo regiões especìficas para a expressão de proteìnas ou epìtopos recombinantes de rotavìrus e astrovìrus, processo de produção dos insumos para geração de imunobiológicos para diagnóstico |
EP1991262A4 (en) * | 2006-03-03 | 2009-11-04 | Merial Ltd | MYCOPLASMA HYOPNEUMONIAE VACCINE |
CA2726131C (en) | 2008-05-29 | 2018-03-13 | Baoming Jiang | Expression and assembly of human group c rotavirus-like particles and uses thereof |
EP2310511B1 (fr) | 2008-07-18 | 2015-01-14 | Sanofi Pasteur | Vecteur auto replicatif depourvu de gene de resistance a un antibiotique |
PL2475384T3 (pl) | 2009-09-10 | 2017-02-28 | Merial, Inc. | Nowe formulacje szczepionek obejmujące adiuwanty zawierające saponiny |
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Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6673355B1 (en) * | 1995-06-14 | 2004-01-06 | Baylor College Of Medicine | Rotavirus enterotoxin NSP4 and methods of using same |
Non-Patent Citations (2)
Title |
---|
EL-ATTAR L: ""Comparison of the efficacy of rotavirus VLP vaccines to a live homologous rotavirus vaccine in a pig model of rotavirus disease."", 《VACCINE.》 * |
XINYUAN QIAO: ""Recombinant porcine rotavirus VP4 and VP4-LTB expressed in Lactobacillus casei induced mucosal and systemic antibody responses in mice"", 《BMC MICROBIOL.》 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN108864260A (zh) * | 2018-07-17 | 2018-11-23 | 昆明学院 | 非结构蛋白nsp4、重组杆状病毒液及制备、粘膜佐剂 |
CN114875047A (zh) * | 2022-05-27 | 2022-08-09 | 江苏三仪生物工程有限公司 | 一种优化的猪轮状病毒外衣壳蛋白vp4的重组表达及应用 |
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