CN104173236B - Terpene aromatic antibacterial alcohol-based hand sanitizer - Google Patents
Terpene aromatic antibacterial alcohol-based hand sanitizer Download PDFInfo
- Publication number
- CN104173236B CN104173236B CN201410404791.4A CN201410404791A CN104173236B CN 104173236 B CN104173236 B CN 104173236B CN 201410404791 A CN201410404791 A CN 201410404791A CN 104173236 B CN104173236 B CN 104173236B
- Authority
- CN
- China
- Prior art keywords
- percent
- natural
- linalool
- carbomer
- white
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims abstract description 30
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 23
- 235000007586 terpenes Nutrition 0.000 title claims abstract description 17
- CDOSHBSSFJOMGT-UHFFFAOYSA-N linalool Chemical compound CC(C)=CCCC(C)(O)C=C CDOSHBSSFJOMGT-UHFFFAOYSA-N 0.000 claims abstract description 30
- 239000010624 camphor oil Substances 0.000 claims abstract description 24
- 229960000411 camphor oil Drugs 0.000 claims abstract description 24
- 240000003377 Shepherdia canadensis Species 0.000 claims abstract description 13
- 235000018324 Shepherdia canadensis Nutrition 0.000 claims abstract description 13
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 claims abstract description 13
- 229940049638 carbomer homopolymer type c Drugs 0.000 claims abstract description 11
- 229940043234 carbomer-940 Drugs 0.000 claims abstract description 11
- 239000000341 volatile oil Substances 0.000 claims abstract description 11
- 239000001490 (3R)-3,7-dimethylocta-1,6-dien-3-ol Substances 0.000 claims abstract description 10
- CDOSHBSSFJOMGT-JTQLQIEISA-N (R)-linalool Natural products CC(C)=CCC[C@@](C)(O)C=C CDOSHBSSFJOMGT-JTQLQIEISA-N 0.000 claims abstract description 10
- 229930007744 linalool Natural products 0.000 claims abstract description 10
- 238000002360 preparation method Methods 0.000 claims abstract description 10
- 238000003756 stirring Methods 0.000 claims abstract description 8
- 239000002994 raw material Substances 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 33
- 150000003505 terpenes Chemical class 0.000 claims description 14
- 230000000845 anti-microbial effect Effects 0.000 claims description 13
- 239000012153 distilled water Substances 0.000 claims description 12
- NIXOWILDQLNWCW-UHFFFAOYSA-N Acrylic acid Chemical compound OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 6
- 229920002125 Sokalan® Polymers 0.000 claims description 6
- 229960001631 carbomer Drugs 0.000 claims description 6
- 238000004040 coloring Methods 0.000 claims description 5
- 239000000470 constituent Substances 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 4
- 239000003921 oil Substances 0.000 claims description 4
- 239000002904 solvent Substances 0.000 claims description 4
- 241000723346 Cinnamomum camphora Species 0.000 claims description 3
- 239000003599 detergent Substances 0.000 claims description 3
- 239000013589 supplement Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 8
- 239000000049 pigment Substances 0.000 abstract description 5
- 230000007935 neutral effect Effects 0.000 abstract description 2
- 239000000443 aerosol Substances 0.000 abstract 1
- 230000005923 long-lasting effect Effects 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 230000001502 supplementing effect Effects 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 description 18
- 238000012360 testing method Methods 0.000 description 12
- 239000000499 gel Substances 0.000 description 7
- 241000588724 Escherichia coli Species 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 241000222122 Candida albicans Species 0.000 description 5
- 241000191967 Staphylococcus aureus Species 0.000 description 5
- 229940095731 candida albicans Drugs 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 230000005764 inhibitory process Effects 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 241000193830 Bacillus <bacterium> Species 0.000 description 3
- 150000001298 alcohols Chemical class 0.000 description 3
- 230000003385 bacteriostatic effect Effects 0.000 description 3
- 239000002537 cosmetic Substances 0.000 description 3
- 238000000151 deposition Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 241001478240 Coccus Species 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- 235000009754 Vitis X bourquina Nutrition 0.000 description 2
- 235000012333 Vitis X labruscana Nutrition 0.000 description 2
- 240000006365 Vitis vinifera Species 0.000 description 2
- 235000014787 Vitis vinifera Nutrition 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000009833 condensation Methods 0.000 description 2
- 230000005494 condensation Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 2
- 235000011187 glycerol Nutrition 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 238000007711 solidification Methods 0.000 description 2
- 230000008023 solidification Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 description 1
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 208000002874 Acne Vulgaris Diseases 0.000 description 1
- 241001116389 Aloe Species 0.000 description 1
- 241000205585 Aquilegia canadensis Species 0.000 description 1
- 235000005979 Citrus limon Nutrition 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- 201000004624 Dermatitis Diseases 0.000 description 1
- 208000004232 Enteritis Diseases 0.000 description 1
- 206010017533 Fungal infection Diseases 0.000 description 1
- 240000000950 Hippophae rhamnoides Species 0.000 description 1
- 235000003145 Hippophae rhamnoides Nutrition 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 208000031888 Mycoses Diseases 0.000 description 1
- 241000588769 Proteus <enterobacteria> Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 206010000496 acne Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 235000011399 aloe vera Nutrition 0.000 description 1
- KAOMOVYHGLSFHQ-UTOQUPLUSA-N anacardic acid Chemical compound CCC\C=C/C\C=C/CCCCCCCC1=CC=CC(O)=C1C(O)=O KAOMOVYHGLSFHQ-UTOQUPLUSA-N 0.000 description 1
- 235000014398 anacardic acid Nutrition 0.000 description 1
- ADFWQBGTDJIESE-UHFFFAOYSA-N anacardic acid 15:0 Natural products CCCCCCCCCCCCCCCC1=CC=CC(O)=C1C(O)=O ADFWQBGTDJIESE-UHFFFAOYSA-N 0.000 description 1
- 230000001857 anti-mycotic effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 239000002543 antimycotic Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 210000001339 epidermal cell Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 235000010445 lecithin Nutrition 0.000 description 1
- 239000000787 lecithin Substances 0.000 description 1
- 229940067606 lecithin Drugs 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012009 microbiological test Methods 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 239000006916 nutrient agar Substances 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 235000019260 propionic acid Nutrition 0.000 description 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000012898 sample dilution Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cosmetics (AREA)
- Detergent Compositions (AREA)
Abstract
The invention discloses a terpene aromatic antibacterial alcohol-based hand sanitizer and a preparation method thereof. The terpene aromatic antibacterial alcohol-based hand sanitizer is prepared from the following raw materials of 0.1-0.3 percent of 97 percent natural linalool, 0.2-1.0 percent of soapberry essential oil, 2.0-5.0 percent of white camphor oil, 5.0-40.0 percent of linalool solution, 0.15-0.3 percent of Carbomer-940, 0.15-0.30 percent of triethanolamine and 0.005-0.015 percent of natural pigments. The preparation method comprises the following steps: supplementing 99 percent ethanol to 100 percent, adding the Carbomer-940 into the linalool solution, slowly adding the natural linalool while stirring, so that the materials are fully mixed; adding the triethanolamine for regulating the pH value to be neutral, adding the soapberry essential oil, the white camphor oil and natural pigments, fully and uniformly stirring, and filling the solution into an aerosol container. The hand sanitizer has natural aromaticity, good effect and long-lasting antibacterial action, takes the linalool solution as one of the raw materials and is comprehensive in utilization, rich in source and low in price.
Description
Technical field
The present invention relates to hand cleanser field, specifically a kind of terpene fragrant antimicrobial non-water hand cleanser.
Background technology
In the increasingly serious today's society of chemical contamination, consumer also requires man and nature while product function is focused on
Harmony, masses call health, the environmental type cleaning supplies of fashion to come out, and " returning to the Nature " becomes and wash one's hands now
One trend of liquid development.Containing has natural plant composition, the hand cleanser products with maintenance effect to be favored by consumer.According to
Report, the hand cleanser products that existing company of foreign countries develops, natural component content reaches 95%～99%, is doted on by consumer
Love.Now some producers of the country are added to some plant extraction liquids or Chinese herbal medicine extract component in hand cleanser, such as aloe,
The extracted form natural plant composition such as sea-buckthorn, lemon juice, marine alga and honeysuckle, these compositions both can whitening and softening skin, people can again looked for
To the nice feeling for going back to nature, there is great attraction to consumer.
Water-free type hand cleanser is a kind of new sterile products, need not be rinsed with water after use, is suitable in water shortage bar
Hand is cleaned under part and sterilization used, it is easy to use, be applied to hospital clinically, public place, geology surveys
Spy, field work and daily life.Therefore, such product because receiving vast consumption deeply the features such as its is easy to use, antibacterial effective
Favor, in recent years sales volume is very big always.
The key component of non-water hand cleanser includes sterilizability composition and volatile ingredient, is usually added into some lower boiling alcohol
Class solvent, its principle is the cell membrane that the antibacterial activity such as " alcohols " factor can effectively destroy bacterium, so that bacterial death, and
Come off from skin, just as the epidermal cell of aging comes off from skin.And alcohols is volatile, will not remain, therefore use
Any residuals are will not leave behind on skin, and it is almost nonirritant to skin, also can seldom cause allergy or contact skin
Inflammation, in order to protect hand skin, also adds the moisturizing ingredients such as glycerine, polyethylene glycol (PEG) in many water-free type hand cleanser.
In the market simplest water-free type hand sanitizer formula is mixed using 75% glycerine of the alcohol with 20%, its
Effect is washing and disinfective action.Also there is the ethanol and the non-water hand cleanser of TCC preparation using 52%, to large intestine
The bacterial reproduction such as bacillus and staphylococcus aureus has good killing action.
There is linalool antibacterial, antiviral and calm etc. to act on, to Escherichia coli, proteus, enteritis film bacillus, grape
Coccus, S. cervisiae, white have good inhibiting effect containing coccus, black-koji mould etc., while linalool also has to propionic acid
The antibacterial activity of bacterium, it can also be used to treat acne, linalool has synergistic function to the anti-hammer mutant bacteria of anacardic acid.Fragrant camphor tree
Alcohol and its acetate can suppress fungal spore or mycelial growth, can be used for fungal infection or share with other antimycotic raw materials
As mould inhibitor.
The purpose of the present invention is to provide a kind of non-water hand cleanser using linalool as active component in addition, increases Non-water washing
The species of hand liquid.
The content of the invention
It is an object of the invention to provide a kind of terpene fragrant antimicrobial non-water hand cleanser, to solve above-mentioned background technology in carry
The problem for going out.
For achieving the above object, the present invention provides following technical scheme：
A kind of terpene fragrant antimicrobial non-water hand cleanser, is formulated by the raw material of following mass percent：97% natural virtue
Camphor tree alcohol 0.1%～0.3%, soapberry essential oil 0.2%～1.0%, camphor oil white 2.0%～5.0% refines camphor oil white condensed
Distilled water 5.0%～40.0%, Carbomer-940 0.15%～0.3%, triethanolamine 0.15%～0.30%, natural colouring matter
0.005%～0.015%, 99% ethanol is supplemented to 100%.
As further scheme of the invention：The natural linalool as Antibacterial Constituents, the natural linalool
As Antibacterial Constituents, the soapberry essential oil as detergent, the camphor oil white as synergist, the refinement camphor oil white
Condensed distilled water as solvent, the Carbomer-940 as gel, the triethanolamine as nertralizer, the second
Alcohol is used as volatilizer.
A kind of preparation method of terpene fragrant antimicrobial non-water hand cleanser, comprises the following steps that：(1) to refinement camphor oil white condensation
Carbomer-940 is slowly added in distilled water afterwards, is stirred while adding, prevented little grumeleuse from generating, prepare carbomer gel molten
Liquid is standby；(2) natural linalool is slowly added to in the carbomer gel solution for preparing, is stirred while adding, be allowed to abundant
10～12h is placed after dissolving mixing；(3) add triethanolamine to the solution in step (2) and be stirred continuously, adjust the pH of solution
Then value sequentially adds natural colouring matter, soapberry essential oil and camphor oil white between 6～7, and 99% second is supplemented with after stirring
Alcohol is fully stirred evenly to 100%.
Compared with prior art, the invention has the beneficial effects as follows：Hand cleanser of the present invention is with natural plant essential oil terpene as work
Property composition, both with natural fragrance, and the non-water hand cleanser with antibacterial activity, can reduce Escherichia coli on hand, golden yellow
Color grape bacillus and Candida albicans etc., with more lasting antibacterial action；Using refine the condensed distilled water of camphor oil white as
One of raw material, it is comprehensive utilization, abundance, cheap.
Specific embodiment
The technical scheme of this patent is described in more detail with reference to specific embodiment.
Embodiment 1
By taking the terpene fragrant antimicrobial non-water hand cleanser of 500g as an example, by the natural linalools of 0.5g 97%, 2.5g soapberrys essence
Oil, the condensed distilled water of 1.5g camphor oil whites, 100g refinement camphor oil white, 1g Carbomer-940s, 0.8g triethanolamines and 0.05g days
So pigment is formulated, and is supplemented with 99% ethanol to 500g.
A kind of preparation method of terpene fragrant antimicrobial non-water hand cleanser, comprises the following steps that：(1) to refinement camphor oil white condensation
Carbomer-940 is slowly added in distilled water afterwards, is stirred while adding, prevented little grumeleuse from generating, prepare carbomer gel molten
Liquid is standby；(2) natural linalool is slowly added to in the carbomer gel solution for preparing, is stirred while adding, be allowed to abundant
12h is placed after dissolving mixing；(3) add triethanolamine to the solution in step (2) and be stirred continuously, the pH value for adjusting solution exists
Between 6～7, natural colouring matter, soapberry essential oil and camphor oil white are then sequentially added, 99% ethanol is supplemented with after stirring extremely
100%, sprayer is loaded after fully stirring evenly, obtain final product terpene fragrant antimicrobial non-water hand cleanser.
Embodiment 2
By taking the terpene fragrant antimicrobial non-water hand cleanser of 500g as an example, by the natural linalools of 1.5g 97%, 5g soapberrys essence
Oil, the condensed distilled water of 2.5g camphor oil whites, 100g refinement camphor oil white, 1g Carbomer-940s, 0.8g triethanolamines and 0.05g days
So pigment is formulated, and is supplemented with 99% ethanol to 500g.
Preparation process with embodiment 1 preparation process.
Embodiment 3
By taking the terpene fragrant antimicrobial non-water hand cleanser of 500g as an example, by the natural linalools of 1.0g 97%, 2.5g soapberrys essence
Oil, the condensed distilled water of 1.5g camphor oil whites, 150g refinement camphor oil white, 1g Carbomer-940s, 0.8g triethanolamines and 0.05g days
So pigment is formulated, and is supplemented with 99% ethanol to 500g.
Preparation process with embodiment 1 preparation process.
In above-described embodiment, natural linalool is made as Antibacterial Constituents, soapberry essential oil as detergent, camphor oil white
For synergist, the condensed distilled water of camphor oil white is refined as solvent, used as gel, triethanolamine is as in for Carbomer-940
And agent, used as volatilizer, Carbomer-940 is the gel of cosmetics-stage to ethanol.
Embodiment 4：Human toxicity is tested
Respectively allow 20 men and women testers to try out (daily using twice) 3 above-mentioned embodiment samples, be used in conjunction 3 months,
Excitant or any bad reaction are seen if there is, 1 is the results are shown in Table.
The human toxicity result of the test of table 1
3 above-mentioned embodiment samples are stored at normal temperatures 3 months, do not have excitant or bad reaction, explanation to human body
It is qualified to the toxicity test of human body.
The microbioassay of embodiment 5 is tested
Reference《Cosmetics health specification》Defend method prison and send out [2002] No. 229 Part IV " Micro biological Tests method " and antibacterial
Hand cleanser standard Q/SUTK 4-2006 methods are carried out.
The sample of above-mentioned 3 embodiment is taken, 1/10,1/100,1/1000 and 1/10000 etc. is diluted to, until sample contains
Till bacterium amount is suitable, lecithin Tween-80 nutrient agar is added, sample is sufficiently mixed uniformly with culture medium, treated
After sample plate and the agar solidification that is not added with the empty plate of sample, plate is overturn, put culture (48 in (36 ± 1) DEG C incubator
± 2) after h, calculate bacterium colony concentration；The culture dish observation of clump count≤10 is selected, if there is sheet area of colony to be more than culture dish face
Long-pending 1/2, then abandon without, if less than the 1/2 of culture dish area, can remaining neat clump count it is double, as this culture
The clump count of ware；Each culture dish is observed 3 times, is averaged, and the results are shown in Table 2.Then bacterium colony concentration is calculated by formula below：
Sample size (mL) in bacterium colony concentration (CFU/mL)=(clump count × Sample Dilution multiple on plate)/plate
Amount of bacteria measurement result in the sample of table 2
Note：National standard requires to be bacterium colony concentration (CFU/mL)≤1000CFU/mL).
Test result indicate that, each sample of embodiment 1,2,3 is protected under being (55 ± 5) % in temperature (20 ± 5) DEG C, relative humidity
When depositing 1 day, 7 days, 14 days, 28 days and 60 days, its internal amount of bacteria meets standard requirement, and bacteriostasis meets cosmetics health
Specification.
Embodiment 6：Escherichia coli (ATCC24752) bacteriostatic test
The Escherichia coli for cultivating 18～24h under the conditions of 30 DEG C～35 DEG C respectively are taken, is eluted with appropriate SPSS, turned
In moving to sterile centrifugation tube, 3000～5000r/min centrifugation 5min；Abandon supernatant, plus appropriate SPSS, vortex mixed,
Mensuration absorbance, determines that bacteria suspension concentration is 106～108cfu/mL；The culture medium constant volume for preparing is adjusted after pH value, is sub-packed in
50mL conical flasks, every bottle of dosage 27mL while add 5 to drip Tween-20s mixing, plus stopper and is packed, 121 DEG C of sterilizing 15min；Will
Detection culture medium after sterilizing is placed in constant temperature in 50 DEG C of water-baths, to take out and all pour made bacteria suspension into detection culture after slightly cooling down
In base, firmly shake up (do not produce bubble as far as possible), be then poured into rapidly in the sterilized petri dishes of horizontal positioned, after its solidification, turn over
Relay and put after 1h, be transferred to 4 DEG C of refrigerators, next day uses；Filter paper cuts out the circular filter paper piece for a diameter of 0.6cm, after sterilizing respectively
40min is soaked in the sample reagent of embodiment 1,2,3, the agar plate surface for having prepared is attached under aseptic condition,
Pressing is posted；Paper distance between commutator segments is not less than 24mm, and scraps of paper centre-to-centre spacing plate edge pastes 6, posts not less than the every kind of reagents of 15mm
After the scraps of paper, put it into rapidly in incubator, cultivate at 37 DEG C, next day determines inhibition zone situation, the results are shown in Table 3.
Inhibition of the table 3 to coli bacteria group
Note：" ++ " represents substantially antibacterial, has in obvious antibacterial ring size, ring without bacterium colony.
Table 3 test result indicate that, each sample temperature (20 ± 5) DEG C, relative humidity be (55 ± 5) % preserve 0 month, 3
Individual month, after 6 months, Escherichia coli are respectively provided with stronger bacteriostasis, basically reach the standard of requirement.
Embodiment 7：Staphylococcus aureus (ATCC6538) bacteriostatic test
Test method the results are shown in Table 4 with the experimental program of embodiment 6.
The inhibition that table 4 falls to staphylococcus aureus
Note："+" represents that part is antibacterial, it can be seen that antibacterial ring size, but still has part colony growth in ring；" ++ " represents substantially suppression
Bacterium, has in obvious antibacterial ring size, ring without bacterium colony.
Table 4 test result indicate that, each sample temperature (20 ± 5) DEG C, relative humidity be (55 ± 5) % preserve 0 month, 3
Individual month, after 6 months, the bacteriostasis of only embodiment 1 somewhat weakens, reason be probably linalool content it is micro- be reduced, but protect
After depositing 6 months, it suppresses the effect of staphylococcus aureus also to basically reach the standard of requirement.
Embodiment 8：Candida albicans (ATCC10231) bacteriostatic test
Test method the results are shown in Table 5 with the experimental program of embodiment 6.
The inhibition that table 5 falls to Candida albicans
Note："+" represents that part is antibacterial, it can be seen that antibacterial ring size, but still has part colony growth in ring；" ++ " represents substantially suppression
Bacterium, has in obvious antibacterial ring size, ring without bacterium colony.
Table 5 test result indicate that, each sample temperature (20 ± 5) DEG C, relative humidity be (55 ± 5) % preserve 0 month, 3
Individual month, after 6 months, the bacteriostasis of only embodiment 1 somewhat weakens, reason be probably linalool content it is micro- be reduced, but protect
After depositing 6 months, it suppresses the effect of Candida albicans also to basically reach the standard of requirement.
Effect basic simlarity is tested to the inhibitory action effect and embodiment 5,6 of other bacterial classifications, is not illustrated one by one.
According to《Disinfection technology standard》Regulation, for the product of hand-wrist bones, it is necessary to do Escherichia coli, staphylococcus aureus
With the inhibitory action effect test of Candida albicans, other bacterial classifications can be with Selection experiment.Tested by embodiment 4,5,6,7 and 8
As a result understand, the non-water hand cleanser meets regulation and requires.
Embodiment 9：Stability study
Concrete research contents：The hand cleanser of embodiment 1 is divided into into three parts of samples, be placed in after first part of sample sealing (- 10 ±
DEG C 1) preserve 1,3,6 months in refrigerator；Second sample is put in the insulating box of (54 ± 2) DEG C and preserves 1,3,6 months；3rd
Part is placed on temperature (25 ± 2) DEG C, relative humidity to preserve 6 months under the conditions of (60 ± 10) %, investigates hot test, low temperature examination
Test, under long term test, whether the outward appearance of hand cleanser, pH value, active constituent content change.
Experimental result shows：The storage of three parts of samples through six months, dewatering ability is good, without layering or muddy,
PH value is substantially remained in neutral range, meets requirement of the cosmetics to pH, and the content of active ingredient natural linalool is substantially steady
It is fixed, without significantly fluctuation.
Claims (3)
1. a kind of terpene fragrant antimicrobial non-water hand cleanser, it is characterised in that be formulated by the raw material of following mass percent：
97% natural linalool 0.1%～0.3%, soapberry essential oil 0.2%～1.0%, camphor oil white 2.0%～5.0% refines white camphor tree
The condensed distilled water 5.0%～40.0% of oil, Carbomer-940 0.15%～0.3%, triethanolamine 0.15%～0.30%,
Natural colouring matter 0.005%～0.015%, supplements 99% ethanol to 100%.
2. terpene fragrant antimicrobial non-water hand cleanser according to claim 1, it is characterised in that the natural linalool conduct
Antibacterial Constituents, used as detergent, used as synergist, the refinement camphor oil white is condensed the camphor oil white soapberry essential oil
Distilled water afterwards used as solvent, used as gel, used as nertralizer, make the triethanolamine Carbomer-940 by the ethanol
For volatilizer.
3. a kind of preparation method of terpene fragrant antimicrobial non-water hand cleanser as claimed in claim 1 or 2, it is characterised in that tool
Body step is as follows：(1) Carbomer-940 is slowly added to in the condensed distilled water of refinement camphor oil white, is stirred while adding, prevented
Only there is little grumeleuse to generate, prepare carbomer gel solution for standby；(2) it is slowly added to day in the carbomer gel solution for preparing
So linalool, stirs while adding, and is allowed to place 10～12h after fully dissolving mixing；(3) add to the solution in step (2)
Triethanolamine is simultaneously stirred continuously, and the pH value for adjusting solution sequentially adds natural colouring matter, soapberry essential oil between 6～7, then
And camphor oil white, 99% ethanol is supplemented with after stirring to 100%, fully stir evenly.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410404791.4A CN104173236B (en) | 2014-08-14 | 2014-08-14 | Terpene aromatic antibacterial alcohol-based hand sanitizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410404791.4A CN104173236B (en) | 2014-08-14 | 2014-08-14 | Terpene aromatic antibacterial alcohol-based hand sanitizer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104173236A CN104173236A (en) | 2014-12-03 |
| CN104173236B true CN104173236B (en) | 2017-05-03 |
Family
ID=51954771
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410404791.4A Active CN104173236B (en) | 2014-08-14 | 2014-08-14 | Terpene aromatic antibacterial alcohol-based hand sanitizer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104173236B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107737070A (en) * | 2017-11-16 | 2018-02-27 | 东兴市良佳优农林科技有限公司 | Sterilization, skin care hand cleaning liquid |
| CN111109262A (en) * | 2020-01-14 | 2020-05-08 | 中国电建集团江西省电力建设有限公司 | Low-toxicity environment-friendly compound bactericide applied to circulating cooling water and use method |
| CN111620388A (en) * | 2020-05-11 | 2020-09-04 | 佛山市南海区里水镇经济促进局 | Beeswax industrial water-saving agent and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110206793A1 (en) * | 2010-02-19 | 2011-08-25 | Mary Kay Inc. | Topical skin care formulation |
| CN102803460A (en) * | 2009-06-15 | 2012-11-28 | Gojo工业公司 | Antimicrobial compositions |
-
2014
- 2014-08-14 CN CN201410404791.4A patent/CN104173236B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102803460A (en) * | 2009-06-15 | 2012-11-28 | Gojo工业公司 | Antimicrobial compositions |
| US20110206793A1 (en) * | 2010-02-19 | 2011-08-25 | Mary Kay Inc. | Topical skin care formulation |
Non-Patent Citations (2)
| Title |
|---|
| 无患子属植物的化学成分及生物活性研究进展;徐凯节等;《天然产物研究与开发》;20131231;第25卷;第267-273页、第257页 * |
| 芳樟醇Ｒ-和S-对映异构单体的体外抗菌活性研究;胡小刚等;《第三军医大学学报》;20131015;第35卷(第19期);第2077-2080页，尤其第2077页正文第1段，摘要 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104173236A (en) | 2014-12-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101606896A (en) | New purposes of Folium Bambosae extract and method for making, its skin-care bath composition and method for making | |
| CN110123834A (en) | A kind of hypochlorous acid thimerosal and preparation method thereof | |
| CN110042020B (en) | Essential oil antibacterial handmade soap and preparation method thereof | |
| Ramadani et al. | Antibacterial activity of pineapple peel (Ananas comosus) eco-enzyme against acne bacterias (Staphylococcus aureus and Prapionibacterium acnes) | |
| CN102894001A (en) | Antiseptic and mildew inhibitor composition for wet tissues and production method thereof | |
| JP7003186B2 (en) | Composition containing meso-2,3-butanediol | |
| CN104173236B (en) | Terpene aromatic antibacterial alcohol-based hand sanitizer | |
| CN107737070A (en) | Sterilization, skin care hand cleaning liquid | |
| CN103416403A (en) | New application of pogostone or derivatives of pogostone | |
| CN108938468A (en) | A kind of Chinese prickly ash hand cleanser and preparation method thereof | |
| CN109294762A (en) | A kind of formula and technique of Xinhui citrus reticulata meat fermentation liquid production dish washing liquid | |
| CN103271841B (en) | Traditional Chinese medicine hand washing lotion | |
| CN109602667B (en) | Antibacterial hand sanitizer and preparation method thereof | |
| CN112675086A (en) | Passion fruit hand-washing-free gel hand sanitizer and preparation method thereof | |
| KR102107772B1 (en) | Composition for cleaning dead skin | |
| CN109350570A (en) | A kind of cosmetics preservative and its application based on natural plant composition | |
| Sukamdi et al. | Formula Optimization of Antimicrobial Hand Sanitizer With Lemongrass Essential Oil | |
| CN108653091A (en) | Disposable hand sanitizing fluid and preparation method thereof | |
| CN108210391A (en) | A kind of skin care bacteriostatic hand sanitizer and preparation method thereof | |
| CN104173237B (en) | Plant source terpene anti-bacteria hand-washing solution and preparation method thereof | |
| KR101487987B1 (en) | A eco-friendly detergent or cleaner comprising the glasswort | |
| Debnath et al. | Formulation and Evaluation of Liquid Soap Containing Herbal Antimicrobial Agent. | |
| CN108904398A (en) | The formula and technique of a kind of Xinhui citrus reticulata flower and mandarin orange meat fermentation liquid production hair washing essence | |
| CN107753358A (en) | Preservative containing clove leaf stem cell sap for cosmetics and preparation method thereof | |
| KR20110018166A (en) | Natural antiseptic compositions containing propolis, lavender essetial oil and lemon essential oil |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210118 Address after: 331200 No.1 inner ring road, Yaodu pharmaceutical logistics park, Zhangshu City, Yichun City, Jiangxi Province Patentee after: Jiangxi Jingdong Health Industry Co.,Ltd. Address before: 330045 Jiangxi Agricultural University, Nanchang City, Jiangxi Province Patentee before: JIANGXI AGRICULTURAL University |
|
| TR01 | Transfer of patent right |
Effective date of registration: 20240514 Address after: 331200 No.1 Yanghu High tech Development Zone, Zhangshu City, Yichun City, Jiangxi Province Patentee after: Zhangshu Qiling Pharmaceutical Co.,Ltd. Country or region after: China Address before: 331200 No.1 inner ring road, Yaodu pharmaceutical logistics park, Zhangshu City, Yichun City, Jiangxi Province Patentee before: Jiangxi Jingdong Health Industry Co.,Ltd. Country or region before: China |