CN104173236A - Terpene aromatic antibacterial alcohol-based hand sanitizer and preparation method thereof - Google Patents
Terpene aromatic antibacterial alcohol-based hand sanitizer and preparation method thereof Download PDFInfo
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- CN104173236A CN104173236A CN201410404791.4A CN201410404791A CN104173236A CN 104173236 A CN104173236 A CN 104173236A CN 201410404791 A CN201410404791 A CN 201410404791A CN 104173236 A CN104173236 A CN 104173236A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 16
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Abstract
The invention discloses a terpene aromatic antibacterial alcohol-based hand sanitizer and a preparation method thereof. The terpene aromatic antibacterial alcohol-based hand sanitizer is prepared from the following raw materials of 0.1-0.3 percent of 97 percent natural linalool, 0.2-1.0 percent of soapberry essential oil, 2.0-5.0 percent of white camphor oil, 5.0-40.0 percent of linalool solution, 0.15-0.3 percent of Carbomer-940, 0.15-0.30 percent of triethanolamine and 0.005-0.015 percent of natural pigments. The preparation method comprises the following steps: supplementing 99 percent ethanol to 100 percent, adding the Carbomer-940 into the linalool solution, slowly adding the natural linalool while stirring, so that the materials are fully mixed; adding the triethanolamine for regulating the pH value to be neutral, adding the soapberry essential oil, the white camphor oil and natural pigments, fully and uniformly stirring, and filling the solution into an aerosol container. The hand sanitizer has natural aromaticity, good effect and long-lasting antibacterial action, takes the linalool solution as one of the raw materials and is comprehensive in utilization, rich in source and low in price.
Description
Technical field
The present invention relates to handwashing liquid field, specifically a kind of terpenoid fragrant antimicrobial non-water hand cleanser and preparation method.
Background technology
At the day by day serious society of chemical contamination, consumer also requires the harmony of man and nature when focusing on product function, masses are calling, and environmental type cleaning supplies healthy, fashion are come out, and " returning to the Nature " become a trend of current handwashing liquid development.Containing the hand cleanser products that has natural plant composition, has a maintenance effect is favored by consumer.It is reported, the hand cleanser products that abroad existing company develops, natural component content reaches 95%～99%, is subject to making a pet of of consumer.More domestic producers join some plant extraction liquids or Chinese herbal medicine extracting composition in handwashing liquid now, Aloe for example, the extracted form natural plant compositions such as Fructus Hippophae, Fructus Citri Limoniae juice, Sargassum and Flos Lonicerae, these compositions both can whitening and softening skin, can make again people find the nice feeling going back to nature, there is to great captivation in consumer.
Water-free type handwashing liquid is a kind of novel sterile products, after use, do not need water to rinse, be suitable for, under hydropenia condition, hand is carried out to cleaning and disinfection use, easy to use, be applied to hospital clinically, public place, geological prospecting, field work and daily life.Therefore, this series products is subject to the favor of vast consumption deeply because of its easy to use, antibacterial feature such as effectively, and sales volume is always very large in recent years.
The key component of non-water hand cleanser comprises disappear toxic component and volatile ingredient, conventionally add some lower boiling alcohols solvents, its principle is that the antibacterial activity factors such as " alcohols " can be destroyed the cell wall of antibacterial effectively, thereby make antibacterial dead, and come off from skin, just as old and feeble epidermis cell is from skin comes off.And alcohols is volatile; can be not residual; therefore use and can not leave any residuals on skin; and to skin nonirritant almost; also seldom can cause allergy or contact dermatitis; in order to protect hand skin, in many water-free type handwashing liquid, also add the moisturizing compositions such as glycerol, Polyethylene Glycol (PEG)
The simplest water-free type handwashing liquid formula is to adopt 75% ethanol to mix with 20% glycerol in the market, and its effect is to wash and disinfective action.Also there are the ethanol of employing 52% and the non-water hand cleanser of TCC preparation, the bacterial reproductions such as escherichia coli and staphylococcus aureus are had to good killing action.
That linalool has is antibacterial, antiviral and the effect such as calm, escherichia coli, Bacillus proteus, enteritis film bacillus, staphylococcus, saccharomyces cerevisiae, white are had to good inhibitory action containing coccus, Aspergillus niger etc., linalool also has the antibacterial activity to bacterium acidi propionici simultaneously, also can be used for Acne treatment, linalool has synergistic function to the anti-hammer mutant bacteria of anacardic acid.Linalool and acetate energy Antifungi spore or mycelial growth, can be used for fungal infection or share as antifungus agent with other antifungal raw materials.
The object of the invention is to provide in addition a kind of and using linalool as the non-water hand cleanser of active component, increase the kind of non-water hand cleanser.
Summary of the invention
The object of the present invention is to provide a kind of cost low and there is terpenoid fragrant antimicrobial non-water hand cleanser and the preparation method of durable antibiotic activity, to solve the problem proposing in above-mentioned background technology.
For achieving the above object, the invention provides following technical scheme:
A kind of terpenoid fragrant antimicrobial non-water hand cleanser, preparation of raw material by following mass percent forms: 97% natural linalool 0.1%～0.3%, Fructus Sapindi Mukouossi quintessence oil 0.2%～1.0%, white camphor oil 2.0%～5.0%, Lignum cinnamomi camphorae water 5.0%～40.0%, Carbomer-940 0.15%～0.3%, triethanolamine 0.15%～0.30%, natural pigment 0.005%～0.015%, supplements 99% ethanol to 100%.
As the further scheme of the present invention: described natural linalool is as Antibacterial Constituents, described Fructus Sapindi Mukouossi quintessence oil is as detergent, described white camphor oil is as synergist, described Lignum cinnamomi camphorae water is as solvent, described Carbomer-940 is as gel, described triethanolamine is as nertralizer, and described ethanol is as volatilizer.
As the present invention's scheme further: described Lignum cinnamomi camphorae water is for refining the condensed distilled water of white camphor oil.
A preparation method for terpenoid fragrant antimicrobial non-water hand cleanser, concrete steps are as follows: (1) slowly adds Carbomer-940 in Lignum cinnamomi camphorae water, stirs while adding, and prevented that little grumeleuse from generating, and prepares carbomer gel solution for standby; (2) in the carbomer gel solution preparing, slowly add natural linalool, stir while adding, make it fully to dissolve the rear 10～12h of placement of mixing; (3) to the solution in step (2), add triethanolamine and constantly stir, the pH value of regulator solution, between 6～7, and then adds natural pigment, Fructus Sapindi Mukouossi quintessence oil and white camphor oil successively, after stirring, supplement again 99% ethanol to 100%, fully stir evenly.
Compared with prior art, the invention has the beneficial effects as follows: it is active component that handwashing liquid of the present invention be take natural plant quintessence oil terpenoid, both there is natural fragrance, the non-water hand cleanser again with antibacterial activity, can reduce escherichia coli, golden yellow Fructus Vitis viniferae bacillus and Candida albicans etc. on hand, there is more lasting antibacterial action; Using Lignum cinnamomi camphorae water as one of raw material, fully utilize, originate and be abundant, cheap.
The specific embodiment
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, obviously, described embodiment is only the present invention's part embodiment, rather than whole embodiment.Embodiment based in the present invention, those of ordinary skills, not making the every other embodiment obtaining under creative work prerequisite, belong to the scope of protection of the invention.
Embodiment 1
The terpenoid fragrant antimicrobial non-water hand cleanser of 500g of take is example, formulated by 0.5g97% natural linalool, 2.5g Fructus Sapindi Mukouossi quintessence oil, 1.5g white camphor oil, 100g Lignum cinnamomi camphorae water, 1g Carbomer-940,0.8g triethanolamine and 0.05g natural pigment, then supplement 99% ethanol to 500g.
A preparation method for terpenoid fragrant antimicrobial non-water hand cleanser, concrete steps are as follows: (1) slowly adds Carbomer-940 in Lignum cinnamomi camphorae water, stirs while adding, and prevented that little grumeleuse from generating, and prepares carbomer gel solution for standby; (2) in the carbomer gel solution preparing, slowly add natural linalool, stir while adding, make it fully to dissolve the rear 12h of placement of mixing; (3) to the solution in step (2), add triethanolamine and constantly stir, the pH value of regulator solution is between 6～7, and then add successively natural pigment, Fructus Sapindi Mukouossi quintessence oil and white camphor oil, after stirring, supplement again 99% ethanol to 100%, after fully stirring evenly, pack aerosol apparatus into, obtain terpenoid fragrant antimicrobial non-water hand cleanser.
Embodiment 2
The terpenoid fragrant antimicrobial non-water hand cleanser of 500g of take is example, formulated by 1.5g97% natural linalool, 5g Fructus Sapindi Mukouossi quintessence oil, 2.5g white camphor oil, 100g Lignum cinnamomi camphorae water, 1g Carbomer-940,0.8g triethanolamine and 0.05g natural pigment, then supplement 99% ethanol to 500g.
Preparation process is with the preparation process of embodiment 1.
Embodiment 3
The terpenoid fragrant antimicrobial non-water hand cleanser of 500g of take is example, formulated by 1.0g97% natural linalool, 2.5g Fructus Sapindi Mukouossi quintessence oil, 1.5g white camphor oil, 150g Lignum cinnamomi camphorae water, 1g Carbomer-940,0.8g triethanolamine and 0.05g natural pigment, then supplement 99% ethanol to 500g.
Preparation process is with the preparation process of embodiment 1.
In above-described embodiment, natural linalool is as Antibacterial Constituents, Fructus Sapindi Mukouossi quintessence oil is as detergent, white camphor oil is as synergist, and Lignum cinnamomi camphorae water is as solvent, and Carbomer-940 is as gel, triethanolamine is as nertralizer, ethanol is as volatilizer, and wherein Lignum cinnamomi camphorae water is for refining the condensed distilled water of white camphor oil, the gel that Carbomer-940 is cosmetics-stage.
Embodiment 4: human toxicity experiment
Respectively allow 20 men and women testers try out (using every day twice) in 3 above-mentioned embodiment samples, be used in conjunction 3 months, see if there is zest or any untoward reaction, the results are shown in Table 1.
Table 1 human toxicity result of the test
3 above-mentioned embodiment samples are stored 3 months at normal temperatures, and human body is not had to zest or untoward reaction, and it is qualified to illustrate the toxicity test of human body.
Embodiment 5 microbioassay tests
With reference to < < cosmetics health standard > >, defend that method prison is sent out [2002] No. 229 the 4th parts " Micro biological Tests method " and anti-bacterial hand lotion standard Q/SUTK 4-2006 method is carried out.
Get the sample of 3 above-mentioned embodiment, dilution is 1/10,1/100,1/1000 and 1/10000 etc., until sample bacteria containing amount is suitable, add lecithin tween 80 nutrient agar, sample is fully mixed homogeneously with culture medium, after having sample plate and not adding agar in the empty plate of sample and solidifying, upset plate, put in (36 ± 1) ℃ incubator and cultivate after (48 ± 2) h, calculate bacterium colony concentration; Select the culture dish of clump count≤10 to observe, if there is lamellar area of colony to be greater than 1/2 of culture dish area, abandon need not, if be less than 1/2 of culture dish area, can remaining neat clump count is double, as the clump count of this culture dish; Each culture dish is observed 3 times, averages, and the results are shown in Table 2.Then by the bacterium colony of formula calculating below concentration:
Sample size (mL) in bacterium colony concentration (CFU/mL)=(clump count * diluted sample multiple on plate)/plate
Amount of bacteria measurement result in table 2 sample
Note: national standard requires as bacterium colony concentration (CFU/mL)≤1000 CFU/mL).
Experimental result shows, embodiment 1,2,3 each samples in temperature (20 ± 5) ℃, relative humidity when preserving 1 day, 7 days, 14 days, 28 days and 60 days under (55 ± 5) %, the amount of bacteria conformance with standard requirement that it is inner, bacteriostasis meets cosmetics health standard.
Embodiment 6: escherichia coli (ATCC24752) bacteriostatic test
Get the escherichia coli of cultivating respectively 18～24h under 30 ℃～35 ℃ conditions, with appropriate physiological saline solution eluting, be transferred in aseptic centrifuge tube the centrifugal 5min of 3000～5000r/min; Abandon supernatant, add appropriate physiological saline solution, vortex mixed, measures absorbance, determines that bacteria suspension concentration is 106～108cfu/mL; By after the culture medium standardize solution adjust pH preparing, be sub-packed in 50mL conical flask, every bottle of dosage 27mL adds 5 tween 20s to mix simultaneously, adds stopper packing, 121 ℃ of sterilizing 15min; Detection culture medium after sterilizing is placed in to 50 ℃ of water-bath constant temperature, take out after slightly cooling made bacteria suspension is all poured into and detected in culture medium, firmly shake up (not producing bubble) as far as possible, then in the aseptic plate of rapid impouring horizontal positioned, after it solidifies, upset is placed after 1h, is transferred to 4 ℃ of refrigerators, and used next day; The circular filter paper sheet that it is 0.6cm that filter paper is cut out as diameter soaks 40min respectively after sterilizing in embodiment 1,2,3 sample reagents, is attached to the agar plate surface having prepared under aseptic condition, and pressing posts; Scraps of paper spacing is not less than 24mm, and scraps of paper centre-to-centre spacing plate edge is not less than every kind of reagent of 15mm and pastes 6, posts after the scraps of paper, puts it into rapidly in incubator, at 37 ℃, cultivates, and measures inhibition zone situation next day, the results are shown in Table 3.
The inhibition of table 3 pair coli bacteria group
Note: " ++ " represents obviously antibacterial, has obvious antibacterial ring, ring is interior without bacterium colony.
Table 3 experimental result shows, each sample is that (55 ± 5) % preserves after 0 month, 3 months, 6 months at temperature (20 ± 5) ℃, relative humidity, and escherichia coli are all had to stronger bacteriostasis, substantially reaches requirement standard.
Embodiment 7: staphylococcus aureus (ATCC6538) bacteriostatic test
Test method, with the experimental program of embodiment 6, the results are shown in Table 4.
The inhibition that table 4 pair staphylococcus aureus falls
Note: "+" represents that part is antibacterial, can see antibacterial ring, but still has part colony growth in ring; " ++ " represents obviously antibacterial, has obvious antibacterial ring, and ring is interior without bacterium colony.
Table 4 experimental result shows, each sample is that (55 ± 5) % preserves after 0 month, 3 months, 6 months at temperature (20 ± 5) ℃, relative humidity, only the bacteriostasis of embodiment 1 weakens a little, reason may be that the content of linalool is micro-a minimizing, but preserve after 6 months, its effect that suppresses staphylococcus aureus also reaches requirement standard substantially.
Embodiment 8: Candida albicans (ATCC10231) bacteriostatic test
Test method, with the experimental program of embodiment 6, the results are shown in Table 5.
The inhibition that table 5 pair Candida albicans falls
Note: "+" represents that part is antibacterial, can see antibacterial ring, but still has part colony growth in ring; " ++ " represents obviously antibacterial, has obvious antibacterial ring, and ring is interior without bacterium colony.
Table 5 experimental result shows, each sample is that (55 ± 5) % preserves after 0 month, 3 months, 6 months at temperature (20 ± 5) ℃, relative humidity, only the bacteriostasis of embodiment 1 weakens a little, reason may be that the content of linalool is micro-a minimizing, but preserve after 6 months, its effect that suppresses Candida albicans also reaches requirement standard substantially.
Substantially similar to embodiment 5,6 test effects to the inhibitory action effect of other strain, not explanation one by one.
According to < < disinfection technology standard > >, stipulate, product for hands sterilization, must do the inhibitory action effect test of escherichia coli, staphylococcus aureus and Candida albicans, other strain can be selected test.From embodiment 4,5,6,7 and 8 experimental results, this non-water hand cleanser meets regulation requirement.
Embodiment 9: stability study
Concrete research contents: the handwashing liquid of embodiment 1 is divided into three duplicate samples, and the first duplicate samples sealing is placed in the refrigerator of (10 ± 1) ℃ and preserves 1,3,6 month; The second duplicate samples is put into the calorstat of (54 ± 2) ℃ and is preserved 1,3,6 month; The 3rd part be placed on temperature (25 ± 2) ℃, relative humidity is preserved 6 months under (60 ± 10) % condition, investigates under hot test, low-temperature test, long term test, whether the outward appearance of handwashing liquid, pH value, active constituent content change.
Experimental result shows: three duplicate samples were through the storage of six months, and centrifugal having good stability, does not have layering or muddiness, pH value remains in neutral range substantially, meet the requirement of cosmetics to pH, the content of effective ingredient natural linalool is basicly stable, without significantly fluctuation.
Claims (4)
1. a terpenoid fragrant antimicrobial non-water hand cleanser, it is characterized in that, preparation of raw material by following mass percent forms: 97% natural linalool 0.1%～0.3%, Fructus Sapindi Mukouossi quintessence oil 0.2%～1.0%, white camphor oil 2.0%～5.0%, Lignum cinnamomi camphorae water 5.0%～40.0%, Carbomer-940 0.15%～0.3%, triethanolamine 0.15%～0.30%, natural pigment 0.005%～0.015%, supplements 99% ethanol to 100%.
2. terpenoid fragrant antimicrobial non-water hand cleanser according to claim 1, it is characterized in that, described natural linalool is as Antibacterial Constituents, described Fructus Sapindi Mukouossi quintessence oil is as detergent, described white camphor oil is as synergist, and described Lignum cinnamomi camphorae water is as solvent, and described Carbomer-940 is as gel, described triethanolamine is as nertralizer, and described ethanol is as volatilizer.
3. terpenoid fragrant antimicrobial non-water hand cleanser according to claim 1 and 2, is characterized in that, described Lignum cinnamomi camphorae water is for refining the condensed distilled water of white camphor oil.
4. the preparation method of a terpenoid fragrant antimicrobial non-water hand cleanser as claimed in claim 1 or 2, it is characterized in that, concrete steps are as follows: (1) slowly adds Carbomer-940 in Lignum cinnamomi camphorae water, stirs while adding, prevented that little grumeleuse from generating, and prepares carbomer gel solution for standby; (2) in the carbomer gel solution preparing, slowly add natural linalool, stir while adding, make it fully to dissolve the rear 10～12h of placement of mixing; (3) to the solution in step (2), add triethanolamine and constantly stir, the pH value of regulator solution, between 6～7, and then adds natural pigment, Fructus Sapindi Mukouossi quintessence oil and white camphor oil successively, after stirring, supplement again 99% ethanol to 100%, fully stir evenly.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107737070A (en) * | 2017-11-16 | 2018-02-27 | 东兴市良佳优农林科技有限公司 | Sterilization, skin care hand cleaning liquid |
| CN111109262A (en) * | 2020-01-14 | 2020-05-08 | 中国电建集团江西省电力建设有限公司 | Low-toxicity environment-friendly compound bactericide applied to circulating cooling water and use method |
| CN111620388A (en) * | 2020-05-11 | 2020-09-04 | 佛山市南海区里水镇经济促进局 | Beeswax industrial water-saving agent and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110206793A1 (en) * | 2010-02-19 | 2011-08-25 | Mary Kay Inc. | Topical skin care formulation |
| CN102803460A (en) * | 2009-06-15 | 2012-11-28 | Gojo工业公司 | Antimicrobial compositions |
-
2014
- 2014-08-14 CN CN201410404791.4A patent/CN104173236B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102803460A (en) * | 2009-06-15 | 2012-11-28 | Gojo工业公司 | Antimicrobial compositions |
| US20110206793A1 (en) * | 2010-02-19 | 2011-08-25 | Mary Kay Inc. | Topical skin care formulation |
Non-Patent Citations (3)
| Title |
|---|
| 徐凯节等: "无患子属植物的化学成分及生物活性研究进展", 《天然产物研究与开发》 * |
| 徐宝财等: "《洗涤剂配方工艺手册》", 31 January 2006, 化学工业出版社 * |
| 胡小刚等: "芳樟醇Ｒ-和S-对映异构单体的体外抗菌活性研究", 《第三军医大学学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107737070A (en) * | 2017-11-16 | 2018-02-27 | 东兴市良佳优农林科技有限公司 | Sterilization, skin care hand cleaning liquid |
| CN111109262A (en) * | 2020-01-14 | 2020-05-08 | 中国电建集团江西省电力建设有限公司 | Low-toxicity environment-friendly compound bactericide applied to circulating cooling water and use method |
| CN111620388A (en) * | 2020-05-11 | 2020-09-04 | 佛山市南海区里水镇经济促进局 | Beeswax industrial water-saving agent and preparation method thereof |
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