CN104171743B - A kind of feed additive for chickens and preparation method thereof - Google Patents
A kind of feed additive for chickens and preparation method thereof Download PDFInfo
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- CN104171743B CN104171743B CN201410426573.0A CN201410426573A CN104171743B CN 104171743 B CN104171743 B CN 104171743B CN 201410426573 A CN201410426573 A CN 201410426573A CN 104171743 B CN104171743 B CN 104171743B
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- feed additive
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- activating agent
- radix astragali
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention belongs to field of fodder, be specifically related to a kind of feed additive for chickens and preparation method thereof.The Radix Astragali in the present invention has regulation blood glucose, antioxidation, defying age, antitumor, enhancing human body immunity power effect, improve blood circulation and nutriture, the effects such as the metabolic capacity of regulation body, the effective ingredient of Cortex cinnamomi japonici (Ramulus Cinnamomi) is volatile oil, and it has effect that suppression antibacterial infects；The present invention is by making activating agent by the Radix Astragali, Cortex cinnamomi japonici (Ramulus Cinnamomi) and vitamin C, while improving immunity of livestock, moreover it is possible to be greatly improved effect of feed additive, promotes growth of meat chicken.The present invention is with Pericarpium Citri Reticulatae as principal agent, and with Folium Pini, Folium Ginkgo, Bulbus Allii, Flos Chrysanthemi Indici, Fructus Crataegi, the Cortex Eucommiae and Poria as accessory drugs, main and accessory drugs combines, and the growth of broiler is greatly facilitated, improves the immunity of broiler simultaneously.
Description
Technical field
The invention belongs to field of fodder, be specifically related to a kind of feed additive for chickens and preparation method thereof.
Background technology
Intensive farm broiler, its survival rate depends on effective Disease epizootic.Cause pathogeny imcrobe infection is to cause poultry
The most important reason of disease, pathogenic microorganism is of a great variety, but from the point of view of the microbe species polluting animal food, most important
Pathogen be Salmonella and escherichia coli, Salmonella, e. coli contamination food caused the ill event of the mankind
Paid attention to by the public.For a long time, control Salmonella in breeding production, coli-infection relies primarily on use antibiotic.
Antibacterial and the antibacterial effect of antibiotic is notable, since it is found that the growth promoting function to animal, has been widely used always
In feedstuff, to balance animal intestinal microflora, suppress pathogenic entero becteria, enhancing human body immunity power, improve production performance.
But, antibiotic, since using, because of its residual and drug resistance, has the most extremely been disputed on；Additionally, the abuse of antibiotic can also result in
Intestinal microecology is disorderly and insecondary immunosuppressant.The discovery of Hong Kong the first NDM-1 superbug carrier in 2009, right
The drug resistance of antibiotic has had further to be understood and recognizes, and its using effect is queried the most deeply.From the eighties in last century
Rise, have started to forbid antibiotic as feed additive, 2006, European Union completely forbade and uses antibiotic in aquaculture, is permitted
Many countries the most gradually start limit the use of or disable antibiotic.The Andreas Heddini of Sweden's infectious disease control institute once alerted
Saying, if the impetus of abuse of antibiotics can not effectively be contained, the mankind are likely to return to the front antibiotic epoch.In consideration of it,
Change and send out safety, green feed additive has become the inexorable trend of animal husbandry development with substitute antibiotics.
In recent years, Chinese herbal medicine is with low cost with it, pure natural property, multifunctionality, toxic and side effects are little, have no drug resistance and low residual
The particular advantages such as stay, have the dual function of nutrition and health care concurrently, can cause the vast poultry of China as the succedaneum of antibiotic
The very big concern of hired herdsman author.Modern medicine study shows, Chinese herbal medicine has enhancing human body immunity power, promotes that growth of animal is sent out
Educate, improve the function such as efficiency of feed utilization and carcass quality.But up to now, it is used for promoting growth of meat chicken, improves immunity of meat chickens
Pure green feed additive little.
Summary of the invention
It is an object of the invention to provide a kind of feed additive for chickens and preparation method thereof, it is possible to increase broiler growth speed
Degree, prevention broiler enteritis, raising Mucosal Immunity power, effectively solve antibacterial infection and antibiotic present in feeding of broiler
The problem of excess.
The present invention solves that above-mentioned technical problem be the technical scheme is that a kind of feed additive for chickens, described
Feed additive is made up of Pericarpium Citri Reticulatae, Folium Pini, Folium Ginkgo, Bulbus Allii, Flos Chrysanthemi Indici, Fructus Crataegi, the Cortex Eucommiae, Poria, activating agent and water, and preparation
Needed for feed additive, the mass percent of each component is respectively Pericarpium Citri Reticulatae 20%-30%, Folium Pini 15%-20%, Folium Ginkgo 5%-8%, big
Bulbus Allii 4%-7%, Flos Chrysanthemi Indici 3%-6%, Fructus Crataegi 1%-3%, Cortex Eucommiae 1%-2%, Poria 0.5%-2%, activating agent 25.5%-42%, surplus are water,
Described activating agent is made up of the Radix Astragali, Cortex cinnamomi japonici (Ramulus Cinnamomi) and vitamin C, and the Radix Astragali, Cortex cinnamomi japonici (Ramulus Cinnamomi) and ascorbic mass ratio are 20:20:1.
The preparation method of described feed additive for chickens, comprises the following steps:
Step one, cleaning
Pericarpium Citri Reticulatae, Folium Pini, Folium Ginkgo, Bulbus Allii, Flos Chrysanthemi Indici, Fructus Crataegi, the Cortex Eucommiae, Poria, the Radix Astragali and Cortex cinnamomi japonici (Ramulus Cinnamomi) are respectively put into frequency
For the ultrasonic cleaner of 22kHz cleans, take out after cleaning 3-5min, standby；
Step 2, the preparation of activating agent
1. the Radix Astragali and Cortex cinnamomi japonici (Ramulus Cinnamomi) mixed by the mass ratio of 1:1 and pulverize, crossing 50 mesh sieves afterwards, preparing mixture I, standby；
2. mixture I and vitamin C are pressed the mass ratio mixing of 40:1, prepares mixture II, afterwards mixture II is put
Enter in the Rotary Evaporators that temperature is 55 DEG C and concentrate, until solid content reaches 14 ° of Brix, prepare activating agent；
Step 3, pulverizing
Weigh each component by the mass percent of described each component, and by Pericarpium Citri Reticulatae, Folium Pini, Folium Ginkgo, Bulbus Allii, Flos Chrysanthemi Indici,
Fructus Crataegi, the Cortex Eucommiae and Poria are respectively placed in the baking oven that temperature is 65 DEG C drying, carry out pulverizing and crossing 40 mesh sieves afterwards, after pulverizing
Each component be respectively placed in vacuum be 0.095MPa, temperature be that the vacuum desiccator of 65 DEG C is dried 1h, afterwards will be dried
Each component be respectively put in high-frequency vibration mill and pulverize so that it is particle diameter reaches 22 μm, standby；
Step 4, allotment
Each component after pulverizing is mixed and stirred for uniformly i.e. preparing feed additive for chickens with activating agent and water.
Effect of each component in the present invention: the Radix Astragali has regulation blood glucose, antioxidation, defying age, antitumor, enhancing body are exempted from
Epidemic disease power effect, improves the effects such as blood circulation and nutriture, the metabolic capacity of regulation body；The effective ingredient of Cortex cinnamomi japonici (Ramulus Cinnamomi) is volatilization
Oil, it has effect that suppression antibacterial infects.Pericarpium Citri Reticulatae has regulation gastrointestinal tract, promotes to absorb, suppress inflammation, regulation lipid generation
Thank, anti-lipid peroxidation, regulation blood glucose and the suppression effect such as fungus, Pericarpium Citri Reticulatae is principal agent.Folium Pini rich in aminoacid, vitamin and
The materials such as various trace elements, flavone, esters and sterols, have stimulating animal appetite, promote that growth of animal, Nutrients Digestion are inhaled
Receive and metabolism, improve efficiency of feed utilization, improve meat and improve the effects such as immunity of organisms；Folium Ginkgo main effectively
Composition is flavone, it have protection capillary permeability, reduce serum cholesterol, improve cardiovascular and cerebrovascular circulation, antibacterial, remove
Free radical, antioxidation etc. act on；The effective ingredient Oleum Bulbus Allii of Bulbus Allii, is the general name of sulfur-containing compound, have antibacterial, antibacterial, change
Kind feed quality, promotion grow, activate gastrointestinal mucosa, protect gastrointestinal tract, promote that appetite, acceleration digest, improve meat and carry
High immunity etc. act on；Flos Chrysanthemi Indici has infection, antibacterial, enhancing human body immunity power, treatment effect such as enteritis and removing toxic substances；Fructus Crataegi
Effective ingredient be a kind of compound for vitexin, have anti-cancer, anticancer suppression flavacin, aid digestion, promote gastric juice divide
The effect such as secrete；The Cortex Eucommiae contains a small amount of protein, it is possible to hydrolysis detection is to aminoacid necessary to body, and contains substantial amounts of ore deposit
Matter-element element, has regulation cellular material metabolism, regulating lipid metabolism, broad-spectrum antiseptic, antitumor, antiinflammatory, raising cellular immunization, rush
Enter gastrointestinal peristalsis and the effect such as gastric secretion, anti-stress；Poria contains β-pachyman, chitin, protein, sterol, lecithin
Deng composition, have anticancer, antitumor, antibacterial, prevent the effect such as necrocytosis, enhancing immunity；These medicines are adjuvant drug；Principal agent,
Adjuvant drug share, and can promote growth of animals or poultry, can improve again immunity of livestock.
Beneficial effect
The feed additive for chickens of the present invention, has the mutual matched of Chinese medicine targetedly by various, learns from other's strong points to offset one's weaknesses, mutually
Collaborative, there is good diseases prevention, disease-resistant, promotion growth, improve immunity of meat chickens, and without any side effects.Concrete bag
Include several aspect:
One, the Radix Astragali in the present invention has regulation blood glucose, antioxidation, defying age, antitumor, enhancing human body immunity masterpiece
With, improving the effects such as blood circulation and nutriture, the metabolic capacity of regulation body, the effective ingredient of Cortex cinnamomi japonici (Ramulus Cinnamomi) is volatile oil, it
There is effect that suppression antibacterial infects；The present invention, by the Radix Astragali, Cortex cinnamomi japonici (Ramulus Cinnamomi) and vitamin C are made activating agent, exempts from improving poultry
While epidemic disease power, moreover it is possible to be greatly improved effect of feed additive, promote growth of meat chicken.
Two, the present invention is with Pericarpium Citri Reticulatae as principal agent, is auxiliary with Folium Pini, Folium Ginkgo, Bulbus Allii, Flos Chrysanthemi Indici, Fructus Crataegi, the Cortex Eucommiae and Poria
Medicine, main and accessory drugs combines, and the growth of broiler is greatly facilitated, improves the immunity of broiler simultaneously.
Three, the raw material in the present invention is easy to get, and preparation is simple, and production cost is relatively low, and extensively, safety is high in effect, and benefit shows
Write.
Four, by the combination of various Chinese herbal medicine, it is possible to significantly improve meat chicken production performance, improve intestinal health, raising machine
Body immunity.Meanwhile, this product has the features such as absorption is fast, utilization rate is high, interpolation is convenient, before therefore having wide application
Scape.
Detailed description of the invention
A kind of feed additive for chickens, described feed additive is by Pericarpium Citri Reticulatae, Folium Pini, Folium Ginkgo, Bulbus Allii, Flos Chrysanthemi Indici, mountain
Short, bristly hair or beard, the Cortex Eucommiae, Poria, activating agent and water are made, and the mass percent of each component is respectively Pericarpium Citri Reticulatae needed for preparing feed additive
20%-30%, Folium Pini 15%-20%, Folium Ginkgo 5%-8%, Bulbus Allii 4%-7%, Flos Chrysanthemi Indici 3%-6%, Fructus Crataegi 1%-3%, Cortex Eucommiae 1%-2%, Fu
Siberian cocklebur 0.5%-2%, activating agent 25.5%-42%, surplus are water, and described activating agent is made up of the Radix Astragali, Cortex cinnamomi japonici (Ramulus Cinnamomi) and vitamin C, and the Radix Astragali,
Cortex cinnamomi japonici (Ramulus Cinnamomi) and ascorbic mass ratio are 20:20:1.
The preparation method of described feed additive for chickens, comprises the following steps:
Step one, cleaning
Pericarpium Citri Reticulatae, Folium Pini, Folium Ginkgo, Bulbus Allii, Flos Chrysanthemi Indici, Fructus Crataegi, the Cortex Eucommiae, Poria, the Radix Astragali and Cortex cinnamomi japonici (Ramulus Cinnamomi) are respectively put into frequency
For the ultrasonic cleaner of 22kHz cleans, take out after cleaning 3-5min, standby；
Step 2, the preparation of activating agent
1. the Radix Astragali and Cortex cinnamomi japonici (Ramulus Cinnamomi) mixed by the mass ratio of 1:1 and pulverize, crossing 50 mesh sieves afterwards, preparing mixture I, standby；
2. mixture I and vitamin C are pressed the mass ratio mixing of 40:1, prepares mixture II, afterwards mixture II is put
Enter in the Rotary Evaporators that temperature is 55 DEG C and concentrate, until solid content reaches 14 ° of Brix, prepare activating agent；
Step 3, pulverizing
Weigh each component by the mass percent of described each component, and by Pericarpium Citri Reticulatae, Folium Pini, Folium Ginkgo, Bulbus Allii, Flos Chrysanthemi Indici,
Fructus Crataegi, the Cortex Eucommiae and Poria are respectively placed in the baking oven that temperature is 65 DEG C drying, carry out pulverizing and crossing 40 mesh sieves afterwards, after pulverizing
Each component be respectively placed in vacuum be 0.095MPa, temperature be that the vacuum desiccator of 65 DEG C is dried 1h, afterwards will be dried
Each component be respectively put in high-frequency vibration mill and pulverize so that it is particle diameter reaches 22 μm, standby；
Step 4, allotment
Each component after pulverizing is mixed and stirred for uniformly i.e. preparing feed additive for chickens with activating agent and water.
Embodiment 1:
A kind of feed additive for chickens, described feed additive is by Pericarpium Citri Reticulatae, Folium Pini, Folium Ginkgo, Bulbus Allii, Flos Chrysanthemi Indici, mountain
Short, bristly hair or beard, the Cortex Eucommiae, Poria, activating agent and water are made, and the mass percent of each component is respectively Pericarpium Citri Reticulatae needed for preparing feed additive
20%, Folium Pini 15%, Folium Ginkgo 5%, Bulbus Allii 4%, Flos Chrysanthemi Indici 3%, Fructus Crataegi 1%, the Cortex Eucommiae 1%, Poria 0.5%, activating agent 25.5%, surplus
For water, described activating agent is made up of the Radix Astragali, Cortex cinnamomi japonici (Ramulus Cinnamomi) and vitamin C, and the Radix Astragali, Cortex cinnamomi japonici (Ramulus Cinnamomi) and ascorbic mass ratio are 20:20:
1。
The preparation method of described feed additive for chickens, comprises the following steps:
Step one, cleaning
Pericarpium Citri Reticulatae, Folium Pini, Folium Ginkgo, Bulbus Allii, Flos Chrysanthemi Indici, Fructus Crataegi, the Cortex Eucommiae, Poria, the Radix Astragali and Cortex cinnamomi japonici (Ramulus Cinnamomi) are respectively put into frequency
For the ultrasonic cleaner of 22kHz cleans, take out after cleaning 3-5min, standby；
Step 2, the preparation of activating agent
1. the Radix Astragali and Cortex cinnamomi japonici (Ramulus Cinnamomi) mixed by the mass ratio of 1:1 and pulverize, crossing 50 mesh sieves afterwards, preparing mixture I, standby；
2. mixture I and vitamin C are pressed the mass ratio mixing of 40:1, prepares mixture II, afterwards mixture II is put
Enter in the Rotary Evaporators that temperature is 55 DEG C and concentrate, until solid content reaches 14 ° of Brix, prepare activating agent；
Step 3, pulverizing
Weigh each component by the mass percent of described each component, and by Pericarpium Citri Reticulatae, Folium Pini, Folium Ginkgo, Bulbus Allii, Flos Chrysanthemi Indici,
Fructus Crataegi, the Cortex Eucommiae and Poria are respectively placed in the baking oven that temperature is 65 DEG C drying, carry out pulverizing and crossing 40 mesh sieves afterwards, after pulverizing
Each component be respectively placed in vacuum be 0.095MPa, temperature be that the vacuum desiccator of 65 DEG C is dried 1h, afterwards will be dried
Each component be respectively put in high-frequency vibration mill and pulverize so that it is particle diameter reaches 22 μm, standby；
Step 4, allotment
Each component after pulverizing is mixed and stirred for uniformly i.e. preparing feed additive for chickens with activating agent and water.
Embodiment 2:
A kind of feed additive for chickens, described feed additive is by Pericarpium Citri Reticulatae, Folium Pini, Folium Ginkgo, Bulbus Allii, Flos Chrysanthemi Indici, mountain
Short, bristly hair or beard, the Cortex Eucommiae, Poria, activating agent and water are made, and the mass percent of each component is respectively Pericarpium Citri Reticulatae needed for preparing feed additive
30%, Folium Pini 20%, Folium Ginkgo 8%, Bulbus Allii 7%, Flos Chrysanthemi Indici 6%, Fructus Crataegi 3%, the Cortex Eucommiae 2%, Poria 2%, activating agent 42%, surplus are water,
Described activating agent is made up of the Radix Astragali, Cortex cinnamomi japonici (Ramulus Cinnamomi) and vitamin C, and the Radix Astragali, Cortex cinnamomi japonici (Ramulus Cinnamomi) and ascorbic mass ratio are 20:20:1.
The preparation method of described feed additive for chickens, comprises the following steps:
Step one, cleaning
Pericarpium Citri Reticulatae, Folium Pini, Folium Ginkgo, Bulbus Allii, Flos Chrysanthemi Indici, Fructus Crataegi, the Cortex Eucommiae, Poria, the Radix Astragali and Cortex cinnamomi japonici (Ramulus Cinnamomi) are respectively put into frequency
For the ultrasonic cleaner of 22kHz cleans, take out after cleaning 3-5min, standby；
Step 2, the preparation of activating agent
1. the Radix Astragali and Cortex cinnamomi japonici (Ramulus Cinnamomi) mixed by the mass ratio of 1:1 and pulverize, crossing 50 mesh sieves afterwards, preparing mixture I, standby；
2. mixture I and vitamin C are pressed the mass ratio mixing of 40:1, prepares mixture II, afterwards mixture II is put
Enter in the Rotary Evaporators that temperature is 55 DEG C and concentrate, until solid content reaches 14 ° of Brix, prepare activating agent；
Step 3, pulverizing
Weigh each component by the mass percent of described each component, and by Pericarpium Citri Reticulatae, Folium Pini, Folium Ginkgo, Bulbus Allii, Flos Chrysanthemi Indici,
Fructus Crataegi, the Cortex Eucommiae and Poria are respectively placed in the baking oven that temperature is 65 DEG C drying, carry out pulverizing and crossing 40 mesh sieves afterwards, after pulverizing
Each component be respectively placed in vacuum be 0.095MPa, temperature be that the vacuum desiccator of 65 DEG C is dried 1h, afterwards will be dried
Each component be respectively put in high-frequency vibration mill and pulverize so that it is particle diameter reaches 22 μm, standby；
Step 4, allotment
Each component after pulverizing is mixed and stirred for uniformly i.e. preparing feed additive for chickens with activating agent and water.
Test 1
Choosing the close broiler of upgrowth situation 120, be divided into A, B, C tri-groups, wherein A group uses the feedstuff of the present invention to add
Agent, the B group use present invention removes the feed additive after activating agent, and C group is without feed additive.Every 7 days records once
Upgrowth situation.
After 1 month, growth result is: A group average weight gain 1800g, does not has sufferer phenomenon；B group average weight gain 1300g, has 1
Broiler is ill；C group average weight gain 700g, has 4-5 broiler ill.
Result shows: the feed additive of the present invention is greatly facilitated the growth of broiler, and the activity in feed additive
Agent serves the effect of greatly facilitating, and shows that the feed additive of the present invention has raising immunity of organisms simultaneously, reduces ill
Effect of rate.
Test 2
Chinese herbal feed additive to 2 proportionings according to embodiments of the present invention carries out the test survey of growth performance:
360 1 aa broiler chicken are selected in test, are randomly divided into 6 groups, often 6 repetitions of group, each repetition 10.
Test is grouped as follows:
1st group of not counteracting toxic substances, feeds basal diet, for matched group
2nd group of counteracting toxic substances, feeds basal diet, for infected group
3rd group of counteracting toxic substances, feeds basal diet+50mg/kg bacitracin zinc, for antibiotic group
4th group of counteracting toxic substances, feeds basal diet+0.5% Chinese medicine feed additive, for low dose group
5th group of counteracting toxic substances, feeds basal diet+1% Chinese medicine feed additive, for middle dosage group
6th group of counteracting toxic substances, feeds basal diet+1.5% Chinese medicine feed additive, for high dose group
Test Diet Formula and trophic level are shown in Table 1, and testing material is mash feed.
Daily ration is with reference to national research council (NRC) 1994 standard preparation.Ration ingredient and trophic level are shown in Table 1,
Diet Types is corn-soybean meal diet.
Table 1 normal feedstuff formula and trophic level table (air bells dry basis)
Table 1 Compodition and nutritive value of broiler diets on fed basis
(air dry basis) %
Table 1 normal feedstuff formula and trophic level table
Table 1 Formulation and calculated composition of broiler diets, on
fed basis
Premix formulation is: premix material provides Premix provided following per kg for every kilogram of daily ration
Of diet: ferrum, 60mg;Copper, 7.5mg;Zinc, 65mg;Manganese, 110mg;Iodine, 1.1mg;Selenium, 0.4mg;Vitamin A, 4500IU;
Vitamin D3,1000IU;20mg;Vitamin K, 1.3 mg;Vitamin B1,2.2mg;Vitamin B2,10 mg;Vitamin
B3,10mg;Choline, 400mg;Vitamin B5,50mg;Vitamin B6,4mg;Biotin, 0.04mg;VB11,
1mg;Vitamin B12,1.013mg.
Trophic level is value of calculation.Nutrient levels were the calculated values
Test feeding of broiler family, AA Luoyang, henan Xinan County provides, and weight differences is not notable (P > 0.05).Used by counteracting toxic substances
S. pullonum is purchased from National Veterinary Culture Collection, numbering CVCC533(C79-13).
Cultivate S. pullonum to logarithmic (log) phase in early days, be placed in refrigerated centrifuge and be centrifuged with 6000r/min for 37 DEG C
10min.Abandon supernatant, resuspended with sterile saline, prepare counteracting toxic substances Bacteria suspension.At 3 ages in days, 2-6 group broiler is carried out often
Only 4 × 104CFU fills to feed and infects.1st group of matched group replaces with sterile saline.
Before test, young bird is given up and utensil is rinsed and the sterilization of Caustic soda water spray, then with formalin and potassium permanganate
(every 1 m3Add 30 mL formalin, 15 g potassium permanganate) stifling 12 h, start test after purifying one week.Test chicken uses cage
Supporting, free choice feeding and drinking-water, the principle that few hello duty adds followed by feeding.1-14 age in days temperature controls at 32-34 DEG C, the most weekly
Lower 2 DEG C to 26 DEG C.1-3 d uses 24 h illumination, and 4-20 d uses 18 h illumination, after be gradually decreased to 12 h.Every day cleans
Hen house, routine cleaning drinking water pipeline, and ventilation in time, it is ensured that in hen house, environmental health is good.Immunity program routinely is entered
OK.Timing feeding feedstuff, twice daily.Test observes the mental status of chicken group, the feed intake of record test chicken every day and dead
Die situation.
Test and carry out in JIUYUE, 2013,21 days by a definite date.Raising place is University Of Science and Technology Of He'nan Animal Science And Technology animal
Room.
In test broiler 4 age in days, 7 ages in days, 14 ages in days, 21 age in days time, from often organize often repeat take a build weight
Medium broiler is butchered.With sterilizing cotton thread by under the ligation shear of caecum two ends, ligation port cotton ball soaked in alcohol is sterilized, and is placed in sterilization and holds
Device intestinal microbial population to be measured.Before butchering, broiler to be killed is weighed, be designated as live chickens weight.Cut rapidly abdominal cavity after blood-letting is lethal open, take
Spleen, thymus, fabricius bursa.The spleen of taking-up, thymus, fabricius bursa are weighed, calculates each Immune Organs Index.
In process of the test every day record broiler often organize often repeat feed intake, extremely wash in a pan number of elements, each heavy by each group in 21 ages in days
Retrial is tested broiler and is weighed on an empty stomach, calculates average daily gain, feed-weight ratio and average daily ingestion amount.
Escherichia coli, Salmonella and lactobacillus use colony counting method to measure.After each intestinal contents is thawed, immediately
In aseptic operating platform, take out the whole chymes in broiler caecum, after being stirred by chyme, take food rotten 0.5 g in sterile test tube
In, add physiological saline solution 4.5 mL, vibrate on magnetic force agitator 3-5 min(250 r/min), make 10-1Diluent,
In 1000 r/min centrifuges, centrifugal 10 min, Aspirate supernatant 0.5 mL, add and fill the second of 4.5 mL normal saline
Root test tube carries out 10-2Dilution, and carry out 10 successively-3-10-6Dilution, finds out and most preferably dilutes gradient, more micro-to each dilution factor
Biology carries out inoculated and cultured.The final dilution factor of escherichia coli is 10-3、10-4And 10-5, the final dilution factor of Salmonella is 10-2、
10-3And 10-4, the final dilution factor of lactic acid bacteria is 10-4、10-5And 10-6；Escherichia coli and final three the dilution factor bacterium of Salmonella
Liquid 10 μ L is inoculated on Mai Kangkai and BS culture dish respectively, and aerobic cultivates 24 h；Lactic acid bacteria then uses MRS culture medium, anaerobism
Cultivate 48 h；Two kinds of bacterium cultivation temperature are all 37 DEG C, cultivate and count, by antibacterial number in every gram of intestinal contents after terminating
Logarithm log (CFU) represents.Computing formula is as follows:
Every gram of cecal content clump count=log10(clump count × extension rate × every time dilute sampling milliliter number)/(inoculation
By sample milliliter number × sample grams).
In time testing broiler 4 age in days, 7 ages in days, 14 ages in days, 21 age in days, often organize and take in often repeating in a build weight
Deng broiler, jugular vein blood collection after weighing, allow serum naturally separate out in 4 DEG C, 4 DEG C, 3 500 rpm are centrifuged 15 min and prepare blood
Clearly ,-20 DEG C of freezen protective after subpackage.
Gather jejunum, ileal mucosa, in centrifuge tube, put-20 DEG C of Refrigerator stores, during mensuration, take about 0.3 g mucous membrane tissue
With normal saline dilution, the homogenate of 0.9%, make 10% mucosa homogenate, 3500-4000 r min-1Centrifugal 10 min, take
Clear liquid-20 DEG C preservation, is used for measuring index of correlation.
Diamine oxidase (DAO) vigor measures according to test kit (Bioengineering Research Institute is built up in Nanjing) method.
Secretory immunoglobulin A (SIgA) uses the radioimmunoassay, RIA medicine that Atom High Tech Co., Ltd. is provided
Box measures, and the radioimmunoassay kit that immunoglobulin G (IgG) uses Beijing North Institute of Biological Technology to provide measures.
Test data uses Excel 2007 to carry out preliminary treatment, adds up with SPSS 18.0 software, uses single-factor side
Difference analysis (One-Way ANOVA, Duncan) carries out significance test of difference and multiple comparisons, and result is with " meansigma methods ± standard
Represent by mistake ".
1. the feed additive impact on infecting Salmonella meat chicken production performance
The feeding concentration of Chinese herbal medicine arranges 0.5 %, 1.0 % and tri-gradients of 1.5 %, and sets counteracting toxic substances and not counteracting toxic substances comparison.
The feed additive of different feeding concentrations see table 2 to the impact of salmonella counteracting toxic substances meat chicken production performance:
The impact on infecting Salmonella meat chicken production performance of table 2 feed additive
Note: the different lowercase alphabet of same column shoulder mark shows that significant difference (P < 0.05), different capitalizations represent that difference is the most aobvious
Write (P<0.01), represent that difference is not notable (P>0.05) without letter shoulder mark.
Note: In the same column, values with different small letter
superscripts mean significant difference (P<0.05), and with different capital
letter superscripts mean significant difference (P<0.01), while with no
letter superscript mean no significant difference (P>0.05)。
As shown in Table 2, infect Salmonella make the average daily gain relatively matched group pole of broiler 21 age in days significantly reduce (P <
0.01), but feed-weight ratio and average daily ingestion amount are all without notable change；The feed additive adding various dose significantly improves meat
Chicken 21 age in days average daily gain, has been respectively increased 11.83%, 12.22% and 14.20%(P < 0.01), average daily ingestion amount also has one
Fixed raising (P > 0.05), close with antibiotic group (P > 0.05).
The feed additive impact on infecting Salmonella Intestine of Broiler flora
As shown in Table 3, in infected group broiler 4 age in days, 7 ages in days, 14 age in days caecums, lactobacillus number reduces (P < 0.05), husky
Door Salmonella number increase (P<0.05), 21 age in days lactobacillus numbers still have reduction (P>0.05), Salmonella number increased (P>
0.05), but difference is the most notable.Add feed additive and significantly increase lactic acid bar in broiler 4 age in days, 7 ages in days, 14 age in days caecums
Bacterium number (P < 0.05), reduces Salmonella number (P < 0.05).Lactic acid bacteria in antibiotic age in days each to broiler caecum and sramana
Salmonella all shows the trend (P > 0.05) of suppression.And feed additive optionally shows facilitation (P to lactobacillus
> 0.05), Salmonella is shown inhibitory action (P > 0.05), and more suitable than inhibition with antibiotic group (P > 0.05).
The impact on infecting Salmonella Intestine of Broiler flora of table 3 feed additive
| Group | Lactobacillus lgCFU | Salmonella lgCFU | |
| 4d | Matched group | 9.31±0.07ABb | 8.37±0.02Cc |
| Infect matched group | 9.07±0.20Ba | 10.09±0.14Aa | |
| Antibiotic group | 9.53±0.17ABb | 8.47±0.04Cc | |
| Low dose group | 8.97±0.14Bb | 8.84±0.01BCbc | |
| Middle dosage group | 10.71±0.53Ab | 9.31±0.43ABb | |
| High dose group | 10.79±0.59Ab | 9.37±0.29ABb | |
| 7d | Matched group | 7.76±0.14Aa | 7.21±0.08Cc |
| Infect matched group | 6.36±0.37Bc | 8.37±0.10Aa | |
| Antibiotic group | 6.82±0.24ABb | 6.91±0.06Cc | |
| Low dose group | 7.29±0.19Aab | 7.10±0.07Cc | |
| Middle dosage group | 7.49±0.12Aab | 7.09±0.11Cc | |
| High dose group | 7.62±0.04Aa | 7.06±0.06Bb | |
| 14d | Matched group | 7.27±0.11ABCb | 5.61±0.18ABbc |
| Infect matched group | 6.60±0.08Cc | 7.42±0.20Aa | |
| Antibiotic group | 7.54±0.22ABab | 6.43±0.89ABab | |
| Low dose group | 7.25±0.20BCb | 4.83±0.22Bc | |
| Middle dosage group | 7.47±0.01Aa | 4.79±0.17Bc | |
| High dose group | 7.94±0.02Aa | 4.68±0.17Bc | |
| 21d | Matched group | 6.98±0.06Ab | 6.74±0.14ABb |
| Infect matched group | 6.91±0.09Ab | 7.05±0.31Aab | |
| Antibiotic group | 7.64±0.17Aa | 7.03±0.10Aa | |
| Low dose group | 7.25±0.27Bc | 6.91±0.06ABb | |
| Middle dosage group | 7.35±0.01Aab | 6.84±0.23Bc | |
| High dose group | 7.38±0.01Aab | 6.77±0.13Bc |
Note: the different lowercase alphabet of same column shoulder mark shows that significant difference (P < 0.05), different capitalizations represent that difference is the most aobvious
Write (P<0.01), represent that difference is not notable (P>0.05) without letter shoulder mark.
Note: In the same column, values with different small letter
superscripts mean significant difference (P<0.05), and with different capital
letter superscripts mean significant difference (P<0.01), while with no
letter superscript mean no significant difference (P>0.05).
The feed additive impact on infecting Salmonella broiler Immune Organs Index
As seen from Table 4, the index and spleen index relatively matched group of each age in days of infected group broiler improves (P > 0.05), totally comes
See, infect Salmonella the most notable on the impact of broiler Immune Organs Index.Infected group broiler 21 age in days index and spleen index significantly raises
(P < 0.05), and after adding feed additive, broiler index and spleen index reduce by 47.45%, 51.53% and 52.55%(P respectively <
0.01), and with antibiotic contrast without significant difference (P > 0.05).
The impact (g/kg) on infecting Salmonella broiler Immune Organs Index of table 4 feed additive
| Group | Spleen | Thymus | Fabricius bursa | |
| 4d | Matched group | 0.74±0.07 | 4.30±0.62 | 1.23±0.12 |
| Infect matched group | 0.77±0.03 | 4.09±0.68 | 1.15±0.08 | |
| Antibiotic group | 0.72±0.03 | 3.78±0.52 | 1.39±0.12 | |
| Low dose group | 0.74±0.08 | 3.54±0.29 | 1.37±0.11 | |
| Middle dosage group | 0.73±0.06 | 3.51±0.32 | 1.40±0.11 | |
| High dose group | 0.72±0.06 | 3.49±0.32 | 1.42±0.11 | |
| 7d | Matched group | 0.72±0.07 | 2.84±0.16 | 1.79±0.18 |
| Infect matched group | 0.89±0.12 | 3.10±0.19 | 1.82±0.11 | |
| Antibiotic group | 0.77±0.03 | 2.90±0.33 | 1.45±0.10 | |
| Low dose group | 0.80±0.03 | 2.48±0.17 | 1.62±0.14 | |
| Middle dosage group | 0.81±0.03 | 2.81±0.31 | 1.58±0.12 | |
| High dose group | 0.82±0.01 | 2.84±0.26 | 1.57±0.23 | |
| 14d | Matched group | 0.78±0.08 | 3.78±0.32 | 1.88±0.12 |
| Infect matched group | 1.07±0.20 | 3.47±0.35 | 2.07±0.27 | |
| Antibiotic group | 0.87±0.15 | 3.84±0.18 | 2.28±0.17 | |
| Low dose group | 1.01±0.28 | 3.47±0.23 | 2.08±0.19 | |
| Middle dosage group | 0.98±0.17 | 3.58±0.10 | 2.17±0.10 | |
| High dose group | 0.97±0.17 | 3.68±0.20 | 2.25±0.11 | |
| 21d | Matched group | 0.91±0.11Bb | 1.99±0.30 | 2.28±0.19 |
| Infect matched group | 1.96±0.45Aa | 1.92±0.25 | 2.17±0.27 | |
| Antibiotic group | 1.11±0.26ABb | 2.04±0.21 | 2.24±0.22 | |
| Low dose group | 0.97±0.05Bb | 2.41±0.20 | 2.21±0.17 | |
| Middle dosage group | 0.95±0.11Bb | 2.44±0.26 | 2.22±0.20 | |
| High dose group | 0.93±0.19Bb | 2.45±0.26 | 2.24±0.12 |
Note: the different lowercase alphabet of same column shoulder mark shows that significant difference (P < 0.05), different capitalizations represent that difference is the most aobvious
Write (P<0.01), represent that difference is not notable (P>0.05) without letter shoulder mark.
Note: In the same column, values with different small letter
superscripts mean significant difference (P<0.05), and with different capital
letter superscripts mean significant difference (P<0.01), while with no
letter superscript mean no significant difference (P>0.05).
The feed additive impact on infecting Salmonella serum of broilers DAO vigor
As shown in Table 5, infect Salmonella serum of broilers DAO vigor to have and certain increase (P > 0.05).Interpolation antibiotic,
Various dose Chinese herbal medicine makes serum of broilers DAO vigor significantly reduce (P < 0.05).Serum of broilers DAO also increases and vigor with age in days
There is reduction trend.
Feed additive impact (the U L on infecting Salmonella serum of broilers DAO vigor in table 5-1)
Note: the different lowercase alphabet of same column shoulder mark shows that significant difference (P < 0.05), different capitalizations represent that difference is the most aobvious
Write (P<0.01), represent that difference is not notable (P>0.05) without letter shoulder mark.
Note: In the same column, values with different small letter
superscripts mean significant difference (P<0.05), and with different capital
letter superscripts mean significant difference (P<0.01), while with no
letter superscript mean no significant difference (P>0.05).
The feed additive impact on infecting Salmonella broiler intestinal mucosa IgG, SIgA content
As shown in table 6, the jejunum IgG content of infected group broiler 7 age in days jejunum and ileal mucosa IgG content and 21 ages in days in
Ascendant trend (P > 0.05).Add antibiotic, various dose Chinese herbal medicine makes broiler jejunum and ileal mucosa IgG content have difference
The reduction (P > 0.05) of degree.
Table 6 feed additive impact (the μ g mgprot on infecting Salmonella broiler intestinal mucosa IgG content-1)
Note: the different lowercase alphabet of same column shoulder mark shows that significant difference (P < 0.05), different capitalizations represent that difference is the most aobvious
Write (P<0.01), represent that difference is not notable (P>0.05) without letter shoulder mark.
Note: In the same column, values with different small letter
superscripts mean significant difference (P<0.05), and with different capital
letter superscripts mean significant difference (P<0.01), while with no
letter superscript mean no significant difference (P>0.05).
As shown in table 7, the broiler Jejunal mucosa SIgA content of infected group broiler 7 age in days and 21 ages in days in rising trend (P >
0.05).Add antibiotic, various dose Chinese herbal medicine makes the Jejunal mucosa SIgA content of broiler 7 age in days and 21 ages in days have difference
The reduction (P > 0.05) of degree.
Table 7 feed additive impact (the μ g mgprot on infecting Salmonella broiler intestinal mucosa SIgA content-1)
Note: the different lowercase alphabet of same column shoulder mark shows that significant difference (P < 0.05), different capitalizations represent that difference is the most aobvious
Write (P<0.01), represent that difference is not notable (P>0.05) without letter shoulder mark.
Note: In the same column, values with different small letter
superscripts mean significant difference (P<0.05), and with different capital
letter superscripts mean significant difference (P<0.01), while with no
letter superscript mean no significant difference (P>0.05).
By testing above it follows that the feed additive prepared can improve the production performance of immunological stress broiler, change
Its Immune Organs Index kind, improves lactic acid bacterium number in caecum, reduces the quantity of salmonella, improves gut barrier, improves intestinal
The protective capability of mucosa is especially the most notable with 1.5 % interpolation group effects.
Claims (1)
1. a feed additive for chickens, it is characterised in that: described feed additive by Pericarpium Citri Reticulatae, Folium Pini, Folium Ginkgo, Bulbus Allii,
Flos Chrysanthemi Indici, Fructus Crataegi, the Cortex Eucommiae, Poria, activating agent and water are made, and the percent mass score of each component needed for preparing feed additive
Wei Pericarpium Citri Reticulatae 20%-30%, Folium Pini 15%-20%, Folium Ginkgo 5%-8%, Bulbus Allii 4%-7%, Flos Chrysanthemi Indici 3%-6%, Fructus Crataegi 1%-3%, the Cortex Eucommiae
1%-2%, Poria 0.5%-2%, activating agent 25.5%-42%, surplus are water, and described activating agent is by the Radix Astragali, Cortex cinnamomi japonici (Ramulus Cinnamomi) and vitamin C system
Become, and the Radix Astragali, Cortex cinnamomi japonici (Ramulus Cinnamomi) and ascorbic mass ratio are 20:20:1.
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| CN104543377A (en) * | 2015-02-11 | 2015-04-29 | 向思帆 | Chinese herbal medicine feed additives |
| CN104686840A (en) * | 2015-02-13 | 2015-06-10 | 高深 | Antibiotic-free chicken feed additive and preparation method thereof |
| CN105660543A (en) * | 2016-02-24 | 2016-06-15 | 范健身 | Preparing method for modified breeding additive for medicinal earthworms |
| CN105815563A (en) * | 2016-04-15 | 2016-08-03 | 高宏 | Woody plant feed additive for livestock and poultry, and preparation method of feed additive |
| CN107115435A (en) * | 2017-05-05 | 2017-09-01 | 洛阳市兽药饲料（动物产品）质量检验监测中心 | A kind of broiler chicken immunopotentiator and preparation method thereof |
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| CN101406246B (en) * | 2008-11-25 | 2011-01-26 | 福州康诺生态农业技术服务有限公司 | Chinese herbal medicine feed additive for broiler chickens as well as preparation and use thereof |
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