CN104160020A - 工程改造的微生物及用于微生物油生产的方法 - Google Patents

工程改造的微生物及用于微生物油生产的方法 Download PDF

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Publication number
CN104160020A
CN104160020A CN201280060959.3A CN201280060959A CN104160020A CN 104160020 A CN104160020 A CN 104160020A CN 201280060959 A CN201280060959 A CN 201280060959A CN 104160020 A CN104160020 A CN 104160020A
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cell
vol
fatty acid
lipid
culture
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Chinese (zh)
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G·斯蒂芬诺珀罗斯
M·泰
S·查克拉伯蒂
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Massachusetts Institute of Technology
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Massachusetts Institute of Technology
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/649—Biodiesel, i.e. fatty acid alkyl esters
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    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
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    • C12N9/93—Ligases (6)
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
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    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/0102—Diacylglycerol O-acyltransferase (2.3.1.20)
    • C—CHEMISTRY; METALLURGY
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    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/03—Oxo-acid-lyases (4.1.3)
    • C12Y401/03006—Citrate (pro-3S)-lyase (4.1.3.6)
    • C—CHEMISTRY; METALLURGY
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    • C12Y604/00—Ligases forming carbon-carbon bonds (6.4)
    • C12Y604/01—Ligases forming carbon-carbon bonds (6.4.1)
    • C12Y604/01002—Acetyl-CoA carboxylase (6.4.1.2)
    • C—CHEMISTRY; METALLURGY
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    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/19—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19)
    • C12Y114/19001—Stearoyl-CoA 9-desaturase (1.14.19.1), i.e. DELTA9-desaturase
    • C—CHEMISTRY; METALLURGY
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    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/03—Acyl groups converted into alkyl on transfer (2.3.3)
    • C12Y203/03008—ATP citrate synthase (2.3.3.8)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Oil, Petroleum & Natural Gas (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201280060959.3A 2011-10-19 2012-10-19 工程改造的微生物及用于微生物油生产的方法 Pending CN104160020A (zh)

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US201161548901P 2011-10-19 2011-10-19
US61/548,901 2011-10-19
US201261663263P 2012-06-22 2012-06-22
US61/663,263 2012-06-22
PCT/US2012/061101 WO2013059649A1 (en) 2011-10-19 2012-10-19 Engineered microbes and methods for microbial oil production

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US (2) US8951776B2 (enExample)
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KR (1) KR102036389B1 (enExample)
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CN107904199A (zh) * 2017-11-30 2018-04-13 杭州唯铂莱生物科技有限公司 用于合成山扁豆酸的工程改造的宿主细胞和方法
CN109929870A (zh) * 2019-02-20 2019-06-25 天津大学 糖代谢与脂质代谢协同提高解脂耶氏酵母合成脂肪酸衍生物的产量的应用
WO2019196791A1 (zh) * 2018-04-09 2019-10-17 中国科学院青岛生物能源与过程研究所 生产神经酸的重组酵母菌株及其应用
CN119799810A (zh) * 2025-03-14 2025-04-11 山西鑫晋商生物科技有限公司 基于发酵工艺优化的二酯油微生物发酵方法及系统

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CN105431529A (zh) * 2013-05-03 2016-03-23 德克萨斯大学系统董事会 用于真菌脂质生产的组合物和方法
EP3137616B1 (en) * 2014-05-01 2023-05-31 Ginkgo Bioworks, Inc. Increasing cellular lipid production by increasingthe activity of diacylglycerol acyltransferase and decreasing the activity of triacylglycerol lipase
WO2015184303A1 (en) * 2014-05-29 2015-12-03 Novogy, Inc. Increasing lipid production in oleaginous yeast
KR102311681B1 (ko) 2015-07-28 2021-10-12 삼성전자주식회사 내산성을 갖는 효모 세포, 그를 이용하여 유기산을 생산하는 방법 및 상기 내산성 효모 세포를 생산하는 방법
JP2017039078A (ja) * 2015-08-19 2017-02-23 太平洋セメント株式会社 排水の処理方法
US11142770B2 (en) 2015-10-20 2021-10-12 Massachusetts Institute Of Technology Isolated oleaginous yeast
EP3652300A4 (en) 2017-07-13 2021-06-23 Radici Chimica S.p.A. BIOLOGICAL PROCEDURES FOR MODIFYING CELLULAR CARBON FLOW
WO2019241322A1 (en) * 2018-06-13 2019-12-19 Manus Bio, Inc. Enzymes, methods, and host cells for producing carminic acid
GB202010630D0 (en) * 2020-07-10 2020-08-26 Imp College Innovations Ltd Methods
WO2024097353A1 (en) * 2022-11-04 2024-05-10 Viridos, Inc. Recombinant algae having high biomass and lipid productivity
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CN107904199A (zh) * 2017-11-30 2018-04-13 杭州唯铂莱生物科技有限公司 用于合成山扁豆酸的工程改造的宿主细胞和方法
WO2019196791A1 (zh) * 2018-04-09 2019-10-17 中国科学院青岛生物能源与过程研究所 生产神经酸的重组酵母菌株及其应用
CN109929870A (zh) * 2019-02-20 2019-06-25 天津大学 糖代谢与脂质代谢协同提高解脂耶氏酵母合成脂肪酸衍生物的产量的应用
CN119799810A (zh) * 2025-03-14 2025-04-11 山西鑫晋商生物科技有限公司 基于发酵工艺优化的二酯油微生物发酵方法及系统

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US9862977B2 (en) 2018-01-09
CN109468241A (zh) 2019-03-15
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JP2014530626A (ja) 2014-11-20
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