CN104160011A - 用于核酸扩增反应的微芯片的制造方法 - Google Patents

用于核酸扩增反应的微芯片的制造方法 Download PDF

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Publication number
CN104160011A
CN104160011A CN201380012088.2A CN201380012088A CN104160011A CN 104160011 A CN104160011 A CN 104160011A CN 201380012088 A CN201380012088 A CN 201380012088A CN 104160011 A CN104160011 A CN 104160011A
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CN
China
Prior art keywords
nucleic acid
acid amplification
microchip
reagent solution
amplification reaction
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Pending
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CN201380012088.2A
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English (en)
Chinese (zh)
Inventor
松本真宽
佐藤正树
渡边英俊
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Sony Corp
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Sony Corp
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Publication of CN104160011A publication Critical patent/CN104160011A/zh
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Rigid containers without fluid transport within
    • B01L3/5088—Rigid containers without fluid transport within confining liquids at a location by surface tension, e.g. virtual wells on plates, wires
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/069—Absorbents; Gels to retain a fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
CN201380012088.2A 2012-03-08 2013-01-16 用于核酸扩增反应的微芯片的制造方法 Pending CN104160011A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2012052322 2012-03-08
JP2012-052322 2012-03-08
PCT/JP2013/050652 WO2013132891A1 (ja) 2012-03-08 2013-01-16 核酸増幅反応用マイクロチップの製造方法

Publications (1)

Publication Number Publication Date
CN104160011A true CN104160011A (zh) 2014-11-19

Family

ID=49116378

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201380012088.2A Pending CN104160011A (zh) 2012-03-08 2013-01-16 用于核酸扩增反应的微芯片的制造方法

Country Status (8)

Country Link
US (1) US9545630B2 (https=)
EP (1) EP2824172B1 (https=)
JP (1) JP5987895B2 (https=)
KR (1) KR20140143139A (https=)
CN (1) CN104160011A (https=)
IN (1) IN2014MN01628A (https=)
RU (1) RU2014135538A (https=)
WO (1) WO2013132891A1 (https=)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109844111A (zh) * 2016-12-13 2019-06-04 荣研化学株式会社 微芯片
CN110885902A (zh) * 2018-09-10 2020-03-17 北京亿森宝生物科技有限公司 用于检测猪蓝耳病毒并鉴别从中猪蓝耳病毒高致病经典变异株的冻干微芯片、试剂盒及方法
CN110885899A (zh) * 2018-09-10 2020-03-17 中国动物疫病预防控制中心（农业部屠宰技术中心） 用于鉴别16种禽病病原冻干微芯片、试剂盒及方法

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109477849B (zh) * 2016-03-15 2022-10-21 雅培分子公司 自动分析系统和方法
WO2025094815A1 (ja) * 2023-10-30 2025-05-08 Ｔｏｐｐａｎホールディングス株式会社 流体デバイス

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CN1927394A (zh) * 2006-09-05 2007-03-14 重庆康卫生物科技有限公司 口服重组幽门螺杆菌疫苗及其制备方法
CN1981055A (zh) * 2004-07-02 2007-06-13 英国国防部 使对于核酸扩增有用的试剂稳定的方法
WO2011099251A1 (ja) * 2010-02-10 2011-08-18 ソニー株式会社 核酸増幅反応用マイクロチップ及びその製造方法
CN102224260A (zh) * 2008-09-24 2011-10-19 施特劳斯控股公司 用于检测分析物的试剂盒和装置
JP2012024072A (ja) * 2010-06-22 2012-02-09 Sony Corp 核酸等温増幅反応用マイクロチップ及びその製造方法並びに核酸等温増幅方法

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JPH0775518B2 (ja) * 1989-09-29 1995-08-16 勝 大鶴 ▲高▼血圧、▲高▼脂血症、肥満の予防及び治療に有効な飲食用キノコタンパク質及びその抽出方法
US5556771A (en) 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification
JPH08291078A (ja) 1995-04-21 1996-11-05 M I O:Kk 高血圧及び高脂血症の予防・治療に有効で且つ抗腫瘍作用を有する飲食用キノコタンパク質、及び肥満の予防・治療に有効で且つ抗腫瘍作用を有する飲食用キノコタンパク質、並びにそれらの抽出方法
JPH10234822A (ja) 1997-02-28 1998-09-08 Material Eng Tech Lab Inc 凍結乾燥用容器
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JPWO2010074265A1 (ja) * 2008-12-25 2012-06-21 ユニバーサル・バイオ・リサーチ株式会社 検体の前処理方法、および生体関連物質の測定方法
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CN1981055A (zh) * 2004-07-02 2007-06-13 英国国防部 使对于核酸扩增有用的试剂稳定的方法
CN1927394A (zh) * 2006-09-05 2007-03-14 重庆康卫生物科技有限公司 口服重组幽门螺杆菌疫苗及其制备方法
CN102224260A (zh) * 2008-09-24 2011-10-19 施特劳斯控股公司 用于检测分析物的试剂盒和装置
WO2011099251A1 (ja) * 2010-02-10 2011-08-18 ソニー株式会社 核酸増幅反応用マイクロチップ及びその製造方法
JP2012024072A (ja) * 2010-06-22 2012-02-09 Sony Corp 核酸等温増幅反応用マイクロチップ及びその製造方法並びに核酸等温増幅方法

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109844111A (zh) * 2016-12-13 2019-06-04 荣研化学株式会社 微芯片
CN110885902A (zh) * 2018-09-10 2020-03-17 北京亿森宝生物科技有限公司 用于检测猪蓝耳病毒并鉴别从中猪蓝耳病毒高致病经典变异株的冻干微芯片、试剂盒及方法
CN110885899A (zh) * 2018-09-10 2020-03-17 中国动物疫病预防控制中心（农业部屠宰技术中心） 用于鉴别16种禽病病原冻干微芯片、试剂盒及方法
CN110885902B (zh) * 2018-09-10 2022-10-21 北京亿森宝生物科技有限公司 用于检测猪蓝耳病毒并鉴别从中猪蓝耳病毒高致病经典变异株的冻干微芯片、试剂盒及方法
CN110885899B (zh) * 2018-09-10 2023-04-14 中国动物疫病预防控制中心（农业部屠宰技术中心） 用于鉴别16种禽病病原冻干微芯片、试剂盒及方法

Also Published As

Publication number Publication date
US9545630B2 (en) 2017-01-17
KR20140143139A (ko) 2014-12-15
WO2013132891A1 (ja) 2013-09-12
US20150017318A1 (en) 2015-01-15
RU2014135538A (ru) 2016-03-20
EP2824172A1 (en) 2015-01-14
IN2014MN01628A (https=) 2015-05-15
EP2824172A4 (en) 2015-11-04
JP5987895B2 (ja) 2016-09-07
JPWO2013132891A1 (ja) 2015-07-30
EP2824172B1 (en) 2017-09-06

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Application publication date: 20141119