CN104098374B - Formula and coating method of newly introduced No.1 Oriental goat bean compound seed coating agent - Google Patents
Formula and coating method of newly introduced No.1 Oriental goat bean compound seed coating agent Download PDFInfo
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Abstract
The invention relates to a formula of a newly introduced No.1 Oriental goat bean compound seed film coating agent and a coating method, which mainly comprise the following components in percentage by mass: 0.6 to 4.2 percent of bactericide, 0.02 to 0.13 percent of plant growth regulator, 0.03 to 0.13 percent of micro-fertilizer, 11.8 to 31.6 percent of auxiliary agent, 0.3 to 2.4 percent of warning color dye and the balance of deionized water. The invention relates to a formula of a newly introduced No.1 Oriental goat bean compound seed film coating agent and a coating method, belongs to a special coating agent and a coating process for the newly introduced No.1 Oriental goat bean, is suitable for coating treatment before seeding of the newly introduced No.1 Oriental goat bean seed, can effectively improve seed quality and activity, prevent seedling stage diseases, promote seedling growth, and have higher practical value for planting of a newly introduced No.1 Oriental goat bean cultivation grassland.
Description
Technical Field
The invention relates to a grass seed sowing pretreatment technology, in particular to a formula and a coating method of a Xinzhao No.1 Dongfang mountain sheep bean seed coating agent.
Background
(Dongfang goat bean: (Galega orientalisLam.) belongs to Papilionoideae (Papilionoideae) of Papilionoideae (Leguminosae) of Leguminosae, and is a perennial herb with upright and hollow stem with 7-10 nodes, most of which are purple green stalks and the plant height is 90-120 cm. The stem is provided with 8-13 leaves which are odd feathered compound leaves and consist of 7-15 leaves, the lower leaves are oval, the upper leaves are elliptical or oblong, and the ratio of the stems to the leaves is 1: 1.62. The raceme grows and the corolla is butterfly, and is bluish purple or light purple. The plant original land is a grassland, a forest grassland and a subalpine meadow of Gaucasian mountain and Alla with the altitude of 330m-1800m, has natural distribution in regions of Esaania, Grugia, Dagustan and Asebia, and is a unique plant species in the region. Currently, there are 8 species of the known genus caprine (Galega L), of which the eastern caprine beans have a high feed value and are also called feed caprine beans.
Since 1932, the Oriental goat bean, as a novel winter leguminous forage, was introduced by 33 regions, Central Europe, Canada, Japan, etc., of 6 franchised countries of the former Soviet Union. Introduction experiments were conducted in the hokkaido area in japan and studies showed that: in the area, the Oriental goat beans and Alokang alfalfa (Medicago sativaL. and red clover (Trifolium pratense L.) has the excellent characteristics of high crude protein content, stability, disease resistance and strong insect resistance. In european countries, the oriental goat beans are sometimes used as ornamental plants for landscaping.
The research of the introduction of the oriental goat beans is started in 90 s of the 20 th century in China, and experts in the field of the domestic grass science successively perform introduction tests on the oriental goat beans in Gansu, Xinjiang, Qinghai, Hebei, Heilongjiang, inner Mongolia and other places, so that the oriental goat beans are determined to have strong stress resistance, wide ecological adaptability, rich nutrition, sustainable high yield, rich leaf quantity, good grass quality, good palatability and the likeIs characterized in that. In 2004, the institute of herbage industry of the institute of zootechnics of Sinkiang, the institute of pasture and fodder crops in China approved by the committee for examining and determining the varieties of forage crops, and the newly introduced No.1 Oriental goat bean (the new quoted product of Oriental dynasties)Galega orientalisLam.cv.Xinyin No. 1) is an introduced variety (registration number is 275), and the eastern goat beans are determined to have the advantages of high grass yield, long available year limit, high crude protein and amino acid content, easiness in hay preparation, enjoyment of all kinds of livestock, no tympany of green feed of ruminant livestock, large seed particle size (1.68-2.00 mm), easiness in cleaning, strong stress resistance, strong disease resistance and the like, and are suitable for establishing and planting grasslands in farming and pasturing areas with irrigation conditions in the north of China. The crude protein content of newly introduced No.1 oriental goat bean in the bud stage and the initial flowering stage respectively reaches 24.95 percent and 23.88 percent, the year of production is reached, fresh grass is produced in the year at 64.20t/ha-75.02t/ha, and hay is produced at 12.84t/ha-15.04 t/ha. It is not difficult to see from the aspect of yield and quality that the oriental goat beans are a rare leguminous forage grass and are expected to be combined with other forage grass to change alfalfa (alfalfa) in the forage grass production links in northern areas of ChinaMedicago sativaL.) has a leading position and a single leading pasture variety, has important practical significance for enriching the variety resources of pasture in China, improving the pasture planting benefit and preventing and treating alfalfa diseases and insect pests, and has wide cultivation and utilization prospects.
At present, newly introduced No.1 eastern goat beans are gradually accepted by vast farmers and herders, and the cultivation area is gradually enlarged. However, when newly introduced No.1 Dongfang goat bean is popularized and cultivated, the phenomena of low emergence rate and slow seedling growth often occur, which causes difficulty in production management and increase in sowing cost, and seriously restricts the cultivation, utilization and popularization of the Dongfang goat bean as high-quality pasture.
The seed coating agent is a novel compound pesticide formulation with film forming property, which is prepared by taking high-efficiency systemic insecticide, bactericide, water-retaining agent, herbicide, plant growth regulator, trace elements, microorganisms and the like as active ingredients, taking a film forming agent, a suspending agent, an antifreeze agent, a preservative, a viscosity stabilizer and the like as auxiliary ingredients and carrying out a special processing technology. The seed coating is a processing technology which uses seeds as carriers and uses coating equipment as a means to uniformly and effectively coat a seed coating agent (usually suspension) on the surfaces of the seeds according to a certain proportion. At present, seed coating and seed pelleting are two important directions for the development of seed coating technology. The seed quality is slightly increased after the coating, and the shape is not obviously changed; the shape and size of the pelleted seeds are obviously changed, the mass is generally increased by 3-50 times, and the pelleted seeds are beneficial to mechanical precision seeding. The excellent biological and genetic characteristics of the seeds can be fully reserved and exerted through processing, the technological content and the commodity value of improved seeds can be greatly improved, and the market competitiveness is improved. In the effective period after the seed is coated, the coating components not only provide sufficient nutrients for the germination of the seeds and the growth of seedlings, improve the growth microenvironment of the seedlings, but also can kill germs inside and outside the epidermis of the seeds, prevent the invasion of diseases and pests, realize the plant protection before the sowing and the field sowing with fertilizer, and achieve the effects of protecting and strengthening the seedlings and enhancing the stress resistance. At present, reports related to seed coating technology of main grain crops such as wheat, corn, rice and the like and important economic crops such as soybean, cotton, peanut and the like are frequently reported in China. The research on the coating technology of grass seeds is relatively few, and the research is mostly focused on the king of grass, namely alfalfa. Newly introduced No.1 Oriental goat beans are used as a novel high-quality pasture and are not reported in research related to seed coating.
Disclosure of Invention
The invention aims to provide a formula and a coating method of a newly introduced No.1 Oriental goat bean compound seed film coating agent, the quality and the activity of the newly introduced No.1 Oriental goat bean film-coated seed prepared by the formula and the process are effectively improved, the seedling growth is promoted along with the slow release of the effective components of the coating agent, the seedling growth is improved, the seed loss rate is reduced, and the survival rate of the first-year young plants on the grassland established and cultivated and the biomass of the overground part are greatly improved.
In order to achieve the above object, the present invention employs the following embodiments: the invention relates to a formula of a newly introduced No.1 Oriental goat bean compound seed film coating agent and a coating method, wherein the coating agent comprises the following components in percentage by mass: 0.6 to 4.2 percent of bactericide, 0.02 to 0.13 percent of plant growth regulator, 0.03 to 0.13 percent of micro-fertilizer, 11.8 to 31.6 percent of auxiliary agent, 0.3 to 1.8 percent of warning color dye and deionized water as solvent.
The bactericide is tolclofos-methyl, carbendazim and thiram, and the three components account for the total weight of the bactericide in percentage by mass: 30% -50% and 20% -40%; 20 to 40 percent.
The plant growth regulator is brassinolide.
The micro-fertilizer is sodium molybdate.
The components of the auxiliary agent account for 3-13 percent of Arabic gum, 3-12 percent of starch gum, 2-10 percent of hydroxyethyl cellulose and 0.6-2.1 percent of polyvinylpyrrolidone by weight of the total mass percentage of the film coating agent formula.
The warning color dye is rhodamine B.
The coating method comprises the steps of adding the components in the formula of the newly introduced No.1 Oriental goat bean compound seed film coating agent into deionized water according to a proportion, dissolving and uniformly mixing, mixing the seed film coating agent and seeds according to a mass ratio of 1:30-45, placing the mixture in a glass triangular flask, wherein the total volume of the mixture is not more than 1/3 of the glass triangular flask, and coating on a shaking table.
The volume of the glass triangular bottle is 250-1000ml, the working parameter of the shaking table is 1900-3600rmp, and the time is 20-40 min.
The invention has the beneficial effects that: the invention relates to a formula of a newly introduced No.1 composite seed film coating agent and a coating method, which belong to a film type coating agent special for the newly introduced No.1 seed and a coating process, and the newly introduced No.1 seed film coated seed prepared by the formula and the process has the advantages that the seed quality and the activity are effectively improved due to the fact that germplasm resources are screened and the seed is subjected to treatments such as initiation, physical dormancy barrier breaking and the like, and along with the slow release of effective components of the coating agent, the seedling formation is promoted, the seedling stage stress resistance is improved, the seedling stage growth is improved, the seed loss rate is reduced, and the survival rate of the first-year young plants on the established and cultivated grassland and the biomass of the overground part are greatly improved.
Compared with the reported pretreatment method for the seeds of the eastern goat beans before sowing, the method organically combines links such as seed initiation, water gap complex (water-gap complexes) removal and the like with the seed coating process, has high technical integration level and simple operation, reduces the later field management cost, and is suitable for the establishment, popularization and application of the large-scale newly-introduced No.1 eastern goat bean cultivation grassland.
Detailed Description
The embodiments of the present invention are further illustrated by the following specific examples. It should be noted that: the examples described below include preferred embodiments of the present invention and a portion of the screening assay content. The present invention is not limited to the following examples, and all the formulations and coating methods of the seed coating agent based on the above-mentioned invention are within the scope of the present invention.
Example 1.
The newly introduced No.1 Oriental goat bean compound seed film coating agent comprises the following components in percentage by mass: 0.6% of tolclofos-methyl, 0.6% of carbendazim, 0.8% of thiram, 0.08% of brassinolide, 0.09% of sodium molybdate, 9% of Arabic gum, 9% of starch gum, 6% of hydroxyethyl cellulose, 1.1% of polyvinylpyrrolidone and 0.9% of rhodamine B as suspending dispersing agents, deionized water as a solvent, and finally the volume of the solution is up to 1000 g.
The method for preparing the Xinzhao No.1 oriental goat bean coated seed comprises the following specific steps.
Firstly, preparing an active ingredient solution, respectively weighing 6g of tolclofos-methyl, 6g of carbendazim and 8g of thiram, dissolving in partial deionized water, and allowing a little precipitate to exist; then 0.8g of brassinolide and 0.9g of sodium molybdate are added and stirred evenly to be dissolved completely.
And secondly, preparing an auxiliary agent solution, keeping the temperature of the deionized water constant to 70 ℃, respectively adding 90g of gum arabic, 90g of starch glue and 60g of hydroxyethyl cellulose, and stirring to uniformly dissolve the gum arabic, the starch glue and the hydroxyethyl cellulose.
And thirdly, mixing the solution prepared in the first step and the solution prepared in the second step, adding 11g of suspension dispersant polyvinylpyrrolidone and 11g of rhodamine B9g, supplementing deionized water in a proper amount, fixing the volume until the total mass of the solution is 1000g, and fully stirring and uniformly mixing to form a sticky colloidal rose-red liquid.
Fourthly, mixing the suspension seed coating agent and the cleaned improved variety of the newly introduced No.1 eastern goat beans according to the mass ratio of 1:40, placing the mixture in a 500ml glass triangular flask, setting the total volume of the mixture to be not more than 1/3 of the glass triangular flask, setting the working parameter of a shaking table to be 3000rmp, and coating the mixture on the shaking table for 30 min.
Example 2.
The newly introduced No.1 Oriental goat bean compound seed film coating agent comprises the following components in percentage by mass: 0.4% of tolclofos-methyl, 0.4% of carbendazim, 0.4% of thiram, 0.03% of brassinolide, 0.04% of sodium molybdate, 4% of Arabic gum, 4% of starch gum, 4% of hydroxyethyl cellulose, 0.8% of polyvinylpyrrolidone and 0.9% of rhodamine B as suspending dispersing agents, deionized water as a solvent, and finally the volume of the solution is up to 1000 g.
The method for preparing the Xinzhao No.1 oriental goat bean coated seed comprises the following specific steps.
Step one, preparing an active ingredient solution, respectively weighing 4g of tolclofos-methyl, 4g of carbendazim and 4g of thiram, dissolving in partial deionized water, and allowing a little precipitate to exist; then 0.3g of brassinolide and 0.4g of sodium molybdate are added and stirred evenly to be dissolved completely.
And secondly, preparing an auxiliary agent solution, keeping the temperature of the deionized water constant to about 70 ℃, respectively adding 40g of gum arabic, 40g of starch glue and 40g of hydroxyethyl cellulose, and stirring to uniformly dissolve the gum arabic, the starch glue and the hydroxyethyl cellulose.
And thirdly, mixing the solution prepared in the first step and the solution prepared in the second step, adding 8g of suspension dispersant polyvinylpyrrolidone and 8g of rhodamine B9g, supplementing deionized water in a proper amount, fixing the volume until the total mass of the solution is 1000g, and fully stirring and uniformly mixing to form a sticky colloidal rose-red liquid.
Fourthly, mixing the suspension seed coating agent and the cleaned improved variety of the newly introduced No.1 eastern goat beans according to the mass ratio of 1:30, placing the mixture in a 500ml glass triangular flask, setting the total volume of the mixture to be not more than 1/3 of the glass triangular flask, setting the working parameter of a shaking table to be 3000rmp, and coating the mixture on the shaking table for 30 min.
Example 3.
The newly introduced No.1 Oriental goat bean compound seed film coating agent comprises the following components in percentage by mass: 1.6% of tolclofos-methyl, 1.2% of carbendazim, 1.4% of thiram, 0.12% of brassinolide, 0.12% of sodium molybdate, 11% of Arabic gum, 11% of starch gum, 8% of hydroxyethyl cellulose, 1.6% of polyvinylpyrrolidone and 0.9% of rhodamine B as suspending dispersing agents, deionized water as a solvent, and finally the volume of the solution is up to 1000 g.
The method for preparing the Xinzhao No.1 oriental goat bean coated seed comprises the following specific steps.
Step one, preparing an active ingredient solution, respectively weighing 16g of tolclofos-methyl, 12g of carbendazim and 14g of thiram, dissolving in partial deionized water, and allowing a little precipitate to exist; then adding 1.2g of brassinolide and 1.2g of sodium molybdate, and stirring uniformly to completely dissolve the brassinolide.
And secondly, preparing an auxiliary agent solution, keeping the temperature of the deionized water constant to about 70 ℃, respectively adding 110g of gum arabic, 110g of starch gum and 80g of hydroxyethyl cellulose, and stirring to uniformly dissolve the gum arabic, the starch gum and the hydroxyethyl cellulose.
And thirdly, mixing the solution prepared in the first step and the solution prepared in the second step, adding 16g of suspension dispersant polyvinylpyrrolidone and 9g of rhodamine B, supplementing deionized water in a proper amount, fixing the volume until the total mass of the solution is 1000g, and fully stirring and uniformly mixing to form a sticky colloidal rose-red liquid.
Fourthly, mixing the suspension seed coating agent and the cleaned improved variety of the newly introduced No.1 eastern goat beans according to the mass ratio of 1:45, placing the mixture in a 500ml glass triangular flask, setting the total volume of the mixture to be not more than 1/3 of the glass triangular flask, setting the working parameters of a shaking table to be 3000rmp, and coating the mixture on the shaking table for 35 min.
Example 4.
The newly introduced No.1 Oriental goat bean compound seed film coating agent comprises the following components in percentage by mass: 0.3% of tolclofos-methyl, 0.3% of carbendazim, 0.3% of thiram, 0.1% of brassinolide, 0.1% of sodium molybdate, 9% of Arabic gum, 9% of starch gum, 7% of hydroxyethyl cellulose, 1.0% of polyvinylpyrrolidone and 0.9% of rhodamine B as suspending dispersing agents, deionized water as a solvent, and finally the volume of the solution is up to 1000 g.
The method for preparing the Xinzhao No.1 oriental goat bean coated seed comprises the following specific steps.
Step one, preparing an active ingredient solution, respectively weighing 3g of tolclofos-methyl, 3g of carbendazim and 3g of thiram, dissolving in partial deionized water, and allowing a little precipitate to exist; then adding 1g of brassinolide and 1g of sodium molybdate, and stirring uniformly to completely dissolve the brassinolide.
And secondly, preparing an auxiliary agent solution, keeping the temperature of the deionized water constant to about 70 ℃, respectively adding 90g of gum arabic, 90g of starch gum and 70g of hydroxyethyl cellulose, and stirring to uniformly dissolve the gum arabic, the starch gum and the hydroxyethyl cellulose.
And thirdly, mixing the solution prepared in the first step and the solution prepared in the second step, adding 10g of suspension dispersant polyvinylpyrrolidone and 9g of rhodamine B, supplementing deionized water in a proper amount, fixing the volume until the total mass of the solution is 1000g, and fully stirring and uniformly mixing to form a sticky colloidal rose-red liquid.
Fourthly, mixing the suspension seed coating agent and the cleaned improved variety of the newly introduced No.1 eastern goat beans according to the mass ratio of 1:40, placing the mixture in a 500ml glass triangular flask, setting the total volume of the mixture to be not more than 1/3 of the glass triangular flask, setting the working parameter of a shaking table to be 3000rmp, and coating the mixture on the shaking table for 30 min.
Example 5.
The effect analysis was performed on the newly introduced No.1 Oriental goat bean-coated seeds prepared in the previous 4 examples.
Newly introduced No.1 Oriental goat bean seed source: in the original seed breeding base of the Oriental goat bean of the institute of herbage industry of the Xinjiang academy of zootechnics, all the coated seeds for the test are cleaned seeds of the Xinzhao No.1 Oriental goat bean which is preserved at normal temperature, the thousand seeds are 6.8g, the purity is 95 percent, and the excellence is 92 percent.
Firstly, field test design.
The coated seeds prepared in the preceding 4 examples were subjected to field cultivation trials using a randomized block design with 3 replicates per treatment. The row spacing is 45cm, the row length is 5m, 5 row areas are arranged, the area of a cell is 15 square meters, precision seeding is carried out, the seeding quantity of each cell is 3000 grains, the seeding quantity is about 1kg/667 square meters, the seeding depth is 1cm, drop irrigation is carried out, and protection rows are arranged on the periphery. The control is the newly introduced No.1 Oriental goat bean seed without pretreatment. The test site is a chart wall test station called by grassland research institute of the academy of sciences of animal husbandry in Xinjiang.
Secondly, measuring parameters.
1. Emergence rate: and (4) sowing for 10 days in 4 months, counting the number of the test area every 12 days until the seedling emergence is finished (the plant enters the four-leaf stage), calculating the rate of emergence, and marking all rotten seeds and withered and yellow seedlings as non-emergence. Emergence rate = average number of seedlings/seeding rate per plot × 100%.
2. Aerial biomass: and (4) cutting the soil in a row by taking the cell as a unit in 15 days after 9 months, and measuring the fresh weight of the overground part in a long term every year. Aboveground biomass growth rate = (average aboveground biomass per treatment-average aboveground biomass of control)/average aboveground biomass of control x 100%.
And thirdly, result and analysis.
Table 1 shows the number of seedlings and the data statistics of the biomass of aerial parts.
Table 2 emergence rate and overground part biomass growth rate data statistics table.
And (4) conclusion: the newly introduced No.1 eastern goat bean coated seed prepared by the embodiment has the emergence rate of 77.5-93.1%, and the increase amplitude is 14.0-29.6% compared with the contrast (63.5%); the biomass growth rate of the overground part is 35.6-111.1%. The method can improve the emergence rate of the newly introduced No.1 Dongfang goat bean seeds and the biomass of the overground part of the first young plant in the growing period, thereby providing technical support for the establishment of the planting grassland for the Dongfang goat bean.
Claims (2)
1. The newly introduced No.1 Oriental goat bean compound seed film coating agent is characterized in that: the film coating agent comprises the following components in percentage by mass: 0.6 percent of tolclofos-methyl, 0.6 percent of carbendazim and 0.8 percent of thiram; brassinolide 0.08%; 0.09% of sodium molybdate; 9% of Arabic gum, 9% of starch gum, 6% of hydroxyethyl cellulose, 1.1% of polyvinylpyrrolidone and 0.9% of rhodamine B; the balance being deionized water solvent.
2. The newly introduced No.1 Oriental goat bean compound seed film coating agent is characterized in that: the film coating agent comprises the following components in percentage by mass: 1.6 percent of tolclofos-methyl, 1.2 percent of carbendazim and 1.4 percent of thiram; 0.12% of brassinolide; 0.12 percent of sodium molybdate; 11% of Arabic gum, 11% of starch gum, 8% of hydroxyethyl cellulose and 1.6% of polyvinylpyrrolidone; 0.9 percent of rhodamine B; the balance being deionized water solvent.
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| CN103210940A (en) * | 2013-05-04 | 2013-07-24 | 福建省农业科学院农业生态研究所 | Seed coating specially used for cassia grasses |
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