CN104087522A - 一种外源表达甲酸脱氢酶的酵母工程菌及其构建方法和应用 - Google Patents
一种外源表达甲酸脱氢酶的酵母工程菌及其构建方法和应用 Download PDFInfo
- Publication number
- CN104087522A CN104087522A CN201410306082.2A CN201410306082A CN104087522A CN 104087522 A CN104087522 A CN 104087522A CN 201410306082 A CN201410306082 A CN 201410306082A CN 104087522 A CN104087522 A CN 104087522A
- Authority
- CN
- China
- Prior art keywords
- heterogenous expression
- hydrogenlyase
- engineering bacteria
- fdh
- saccharomyces cerevisiae
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims abstract description 30
- 238000010276 construction Methods 0.000 title claims abstract description 12
- 108090000698 Formate Dehydrogenases Proteins 0.000 title claims abstract description 11
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 title abstract 8
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 27
- 102000004190 Enzymes Human genes 0.000 claims abstract description 18
- 108090000790 Enzymes Proteins 0.000 claims abstract description 18
- 241000235648 Pichia Species 0.000 claims abstract description 6
- 241000894006 Bacteria Species 0.000 claims description 37
- 108010020056 Hydrogenase Proteins 0.000 claims description 29
- 239000013612 plasmid Substances 0.000 claims description 26
- 238000000034 method Methods 0.000 claims description 16
- 108091008146 restriction endonucleases Proteins 0.000 claims description 11
- 241000235058 Komagataella pastoris Species 0.000 claims description 8
- 108090000623 proteins and genes Proteins 0.000 claims description 8
- 238000005215 recombination Methods 0.000 claims description 7
- 230000006798 recombination Effects 0.000 claims description 7
- 230000006698 induction Effects 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 230000000968 intestinal effect Effects 0.000 claims description 4
- 230000035772 mutation Effects 0.000 claims description 4
- 238000012216 screening Methods 0.000 claims description 4
- 239000006228 supernatant Substances 0.000 claims description 4
- 108010084455 Zeocin Proteins 0.000 claims description 3
- 244000005700 microbiome Species 0.000 claims description 3
- CWCMIVBLVUHDHK-ZSNHEYEWSA-N phleomycin D1 Chemical compound N([C@H](C(=O)N[C@H](C)[C@@H](O)[C@H](C)C(=O)N[C@@H]([C@H](O)C)C(=O)NCCC=1SC[C@@H](N=1)C=1SC=C(N=1)C(=O)NCCCCNC(N)=N)[C@@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@H](CO)O1)O[C@@H]1[C@H]([C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O1)O)C=1N=CNC=1)C(=O)C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C CWCMIVBLVUHDHK-ZSNHEYEWSA-N 0.000 claims description 3
- 101710194180 Alcohol oxidase 1 Proteins 0.000 claims description 2
- 238000004321 preservation Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 2
- 238000009629 microbiological culture Methods 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 8
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 8
- 230000005611 electricity Effects 0.000 description 5
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 4
- BAWFJGJZGIEFAR-NNYOXOHSSA-O NAD(+) Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-O 0.000 description 4
- 235000019253 formic acid Nutrition 0.000 description 4
- 241000222124 [Candida] boidinii Species 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 3
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 239000005515 coenzyme Substances 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000000600 sorbitol Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- SNICXCGAKADSCV-JTQLQIEISA-N (-)-Nicotine Chemical compound CN1CCC[C@H]1C1=CC=CN=C1 SNICXCGAKADSCV-JTQLQIEISA-N 0.000 description 1
- MEIRRNXMZYDVDW-MQQKCMAXSA-N (2E,4E)-2,4-hexadien-1-ol Chemical compound C\C=C\C=C\CO MEIRRNXMZYDVDW-MQQKCMAXSA-N 0.000 description 1
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 1
- 101710088194 Dehydrogenase Proteins 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000005815 base catalysis Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000011089 carbon dioxide Nutrition 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 101150086278 fdh gene Proteins 0.000 description 1
- GPRLSGONYQIRFK-UHFFFAOYSA-N hydron Chemical compound [H+] GPRLSGONYQIRFK-UHFFFAOYSA-N 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 229960002715 nicotine Drugs 0.000 description 1
- SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (7)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410306082.2A CN104087522B (zh) | 2014-06-30 | 2014-06-30 | 一种外源表达甲酸脱氢酶的酵母工程菌及其构建方法和应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410306082.2A CN104087522B (zh) | 2014-06-30 | 2014-06-30 | 一种外源表达甲酸脱氢酶的酵母工程菌及其构建方法和应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104087522A true CN104087522A (zh) | 2014-10-08 |
CN104087522B CN104087522B (zh) | 2016-11-09 |
Family
ID=51635284
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201410306082.2A Expired - Fee Related CN104087522B (zh) | 2014-06-30 | 2014-06-30 | 一种外源表达甲酸脱氢酶的酵母工程菌及其构建方法和应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN104087522B (zh) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106834148A (zh) * | 2017-01-20 | 2017-06-13 | 于洪梅 | 一株能降低高级醇含量的啤酒酵母及其应用 |
CN109957520A (zh) * | 2017-12-25 | 2019-07-02 | 丰益(上海)生物技术研发中心有限公司 | 外源基因表达用毕赤酵母菌株 |
CN110408604A (zh) * | 2019-08-14 | 2019-11-05 | 华东理工大学 | 底物亲和力和辅酶亲和力提高的甲酸脱氢酶突变体 |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107299074B (zh) * | 2017-08-30 | 2020-06-16 | 山东省科学院生态研究所 | 甲酸脱氢酶工程菌株的构建方法及应用 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102277370A (zh) * | 2010-05-14 | 2011-12-14 | 昆明理工大学 | 博伊丁假丝酵母甲酸脱氢酶的原核表达载体及其构建方法和应用 |
-
2014
- 2014-06-30 CN CN201410306082.2A patent/CN104087522B/zh not_active Expired - Fee Related
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102277370A (zh) * | 2010-05-14 | 2011-12-14 | 昆明理工大学 | 博伊丁假丝酵母甲酸脱氢酶的原核表达载体及其构建方法和应用 |
Non-Patent Citations (3)
Title |
---|
KRATZER R ET AL.: "Whole-cell bioreduction of aromatic alpha-keto esters using Candida tenuis xylose reductase and Candida boidinii formate dehydrogenase co-expressed in Escherichia coli", 《MICROB CELL FACT.》 * |
余占桥等: "毕赤酵母优化表达外源蛋白策略", 《微生物学通报》 * |
王黎明等: "巴斯德毕赤酵母表达系统研究进展", 《微生物学杂志》 * |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106834148A (zh) * | 2017-01-20 | 2017-06-13 | 于洪梅 | 一株能降低高级醇含量的啤酒酵母及其应用 |
CN109957520A (zh) * | 2017-12-25 | 2019-07-02 | 丰益(上海)生物技术研发中心有限公司 | 外源基因表达用毕赤酵母菌株 |
CN109957520B (zh) * | 2017-12-25 | 2022-12-09 | 丰益(上海)生物技术研发中心有限公司 | 外源基因表达用毕赤酵母菌株 |
CN110408604A (zh) * | 2019-08-14 | 2019-11-05 | 华东理工大学 | 底物亲和力和辅酶亲和力提高的甲酸脱氢酶突变体 |
CN110408604B (zh) * | 2019-08-14 | 2020-11-27 | 华东理工大学 | 底物亲和力和辅酶亲和力提高的甲酸脱氢酶突变体 |
Also Published As
Publication number | Publication date |
---|---|
CN104087522B (zh) | 2016-11-09 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP2013528398A5 (zh) | ||
CN105420252A (zh) | 葡萄糖氧化酶基因god、利用其编码的蛋白、转化的巴斯德毕赤酵母及其制备 | |
CN104087522A (zh) | 一种外源表达甲酸脱氢酶的酵母工程菌及其构建方法和应用 | |
CN103232986B (zh) | 一种异戊二烯生产方法 | |
CN103820347B (zh) | 一株具有乙酸耐受性的工业酿酒酵母菌株 | |
CN101463328A (zh) | 一株利用木糖发酵产乙醇的代谢工程酵母菌 | |
CN105368731B (zh) | 一株表达木糖异构酶的酿酒酵母菌株及构建方法 | |
CN105255951B (zh) | 一种通过过量表达hac1基因提高酒精生产效率的方法 | |
CN101434929A (zh) | 一种睾丸酮丛毛单胞菌工程菌 | |
CN106520653B (zh) | 高产电活性和环境胁迫耐受性的基因工程菌 | |
CN101270156B (zh) | 一种突变的酿酒酵母起始转录因子及其编码基因与应用 | |
CN101979535B (zh) | 等位基因的敲除方法及其构建的重组gal菌株和用途 | |
CN107893043B (zh) | 一种耐受高浓度乙酸的运动发酵单胞菌突变株及其应用 | |
CN102604849A (zh) | 一株高效利用乳糖生产燃料乙醇的酿酒酵母工程菌 | |
CN102154137B (zh) | 一株温度耐受性酿酒酵母及其应用 | |
CN104673737B (zh) | 一株耐乙醇运动发酵单胞菌及其制备方法和应用 | |
CN105255952B (zh) | 一种通过过量表达ino2基因提高酒精生产效率的方法 | |
Buddiwong et al. | Screening of thermotolerant yeast isolated from sugarcane plantations in Northeastern part of Thailand | |
CN103667274A (zh) | 一种多形汉逊酵母遗传操作策略及其应用 | |
CN103289905B (zh) | 一种低阶煤降解菌及其在褐煤液化中的应用 | |
CN102876625A (zh) | 一种产异丁醇的运动发酵单胞菌基因工程菌及其构建方法 | |
CN105199975B (zh) | 一株高效利用乳清产乙醇的酿酒酵母工程菌及其构建方法 | |
CN116716321B (zh) | Hmx1及其编码基因在提高酿酒酵母木糖发酵性能和乙酸耐受性上的应用 | |
CN103320333B (zh) | 携带染色体分散整合木糖基因的工业酿酒酵母重组菌株 | |
CN103088434A (zh) | 树干毕赤酵母大片段dna基因组文库的构建方法及其应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20181011 Address after: 810000 Qinghai Xining biological science and Technology Industrial Park, No. four road 10. Patentee after: FANINON SPECIAL SUGAR INDUSTRY Co.,Ltd. Address before: 210037 Xinzhuang 9, long pan Road, Nanjing, Jiangsu Patentee before: Nanjing Forestry University |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20200210 Address after: 518000 floor 28-03, Weisheng technology building, No. 9966, Shennan Avenue, Maling community, Yuehai street, Nanshan District, Shenzhen City, Guangdong Province Patentee after: Shenzhen hualikang fiber Biotechnology Co.,Ltd. Address before: 810000 Xining biological science and Technology Industrial Park, No. four, No. 10, Qinghai Patentee before: FANINON SPECIAL SUGAR INDUSTRY Co.,Ltd. |
|
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20161109 |